Lcy13g012160 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g012160
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionDDE Tnp4 domain-containing protein
LocationChr13: 15634972 .. 15635256 (+)
RNA-Seq ExpressionLcy13g012160
SyntenyLcy13g012160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGGATATTTAGAACCATATAAAGGAGAGAGATATCACCCTCCTGATTTTCGTCGGGGTAGCCAACCAGTAGGTTATCAAGAGATATTCAACCAAGCACATTCTTCTCTTAGAAGTGTCATTGAGCGAACTTTTGGAATATGGAAGAAGAGGTGGAAGATTCTAAGAGATATATCTAATTATCCTTTTCAAAAGCAAGTGAAGATAATTATTGCAACTATGACACTTCACAACTATATAAGAAGATATGATCGAAGTGATGGTCATTTTGAGAAAGATTGA

mRNA sequence

ATGAAAGGATATTTAGAACCATATAAAGGAGAGAGATATCACCCTCCTGATTTTCGTCGGGGTAGCCAACCAGTAGGTTATCAAGAGATATTCAACCAAGCACATTCTTCTCTTAGAAGTGTCATTGAGCGAACTTTTGGAATATGGAAGAAGAGGTGGAAGATTCTAAGAGATATATCTAATTATCCTTTTCAAAAGCAAGTGAAGATAATTATTGCAACTATGACACTTCACAACTATATAAGAAGATATGATCGAAGTGATGGTCATTTTGAGAAAGATTGA

Coding sequence (CDS)

ATGAAAGGATATTTAGAACCATATAAAGGAGAGAGATATCACCCTCCTGATTTTCGTCGGGGTAGCCAACCAGTAGGTTATCAAGAGATATTCAACCAAGCACATTCTTCTCTTAGAAGTGTCATTGAGCGAACTTTTGGAATATGGAAGAAGAGGTGGAAGATTCTAAGAGATATATCTAATTATCCTTTTCAAAAGCAAGTGAAGATAATTATTGCAACTATGACACTTCACAACTATATAAGAAGATATGATCGAAGTGATGGTCATTTTGAGAAAGATTGA

Protein sequence

MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDISNYPFQKQVKIIIATMTLHNYIRRYDRSDGHFEKD
Homology
BLAST of Lcy13g012160 vs. ExPASy TrEMBL
Match: A0A5C7H626 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_021570 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.2e-37
Identity = 73/93 (78.49%), Postives = 82/93 (88.17%), Query Frame = 0

Query: 1  MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
          M GYL PYKGERYH PDFRRGSQP G+QE+FN AHSSLRSVIERTFG+WKKRWKILRD+ 
Sbjct: 1  MDGYLGPYKGERYHLPDFRRGSQPTGHQEVFNHAHSSLRSVIERTFGVWKKRWKILRDMP 60

Query: 61 NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFEK 94
          +YPF KQVKI+IATM +HNYIRR+ + D HFEK
Sbjct: 61 SYPFDKQVKIVIATMAIHNYIRRHAKHDHHFEK 93

BLAST of Lcy13g012160 vs. ExPASy TrEMBL
Match: M5WCA3 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa017281mg PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.7e-37
Identity = 72/92 (78.26%), Postives = 81/92 (88.04%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M+GYL PYKGERYH PDF RGSQP GY+E+FN  HSSLRSVIERTFG+WKKRWKILRD+ 
Sbjct: 491 MRGYLGPYKGERYHLPDFHRGSQPTGYKEVFNHVHSSLRSVIERTFGVWKKRWKILRDMP 550

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFE 93
           NYPF KQVKI+IATMTLHNYIRR+ + D HF+
Sbjct: 551 NYPFNKQVKIVIATMTLHNYIRRHAQRDRHFD 582

BLAST of Lcy13g012160 vs. ExPASy TrEMBL
Match: A0A5N5FXF0 (DDE Tnp4 domain-containing protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_005777 PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 9.2e-36
Identity = 68/92 (73.91%), Postives = 79/92 (85.87%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M+GYL PYKGERYH PDFRRG++P G++E+FN  HSSLRS+IERTFG+WKKRW ILRD+ 
Sbjct: 257 MRGYLGPYKGERYHLPDFRRGAEPTGHKEVFNHTHSSLRSIIERTFGVWKKRWAILRDMP 316

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFE 93
           NYPF KQVKI+IATM LHNYIRRY   D HF+
Sbjct: 317 NYPFNKQVKIVIATMALHNYIRRYSERDRHFD 348

BLAST of Lcy13g012160 vs. ExPASy TrEMBL
Match: A0A5C7ITA1 (DDE Tnp4 domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000937 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.1e-35
Identity = 69/93 (74.19%), Postives = 79/93 (84.95%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M GYL PYKGERYH PDFR+GSQP G+QE+FN  HSSLRSVIERTFG+WKKRWKILRD+ 
Sbjct: 67  MDGYLGPYKGERYHLPDFRQGSQPTGHQEVFNHTHSSLRSVIERTFGVWKKRWKILRDMP 126

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFEK 94
           +YPF KQVKI+I  M +HNYIRR+ + D HFEK
Sbjct: 127 SYPFDKQVKIVIVNMAIHNYIRRHAKHDHHFEK 159

BLAST of Lcy13g012160 vs. ExPASy TrEMBL
Match: A0A540MIT7 (DDE Tnp4 domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_015721 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.7e-35
Identity = 66/92 (71.74%), Postives = 79/92 (85.87%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M+GYL PYKGERYH PDFRRG++P G++E+FN  HSSLRS+IERTFG+WKK+W ILRD+ 
Sbjct: 160 MRGYLGPYKGERYHLPDFRRGAEPTGHKEVFNHTHSSLRSIIERTFGVWKKKWSILRDMP 219

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFE 93
           NYPF KQVKI+IATM +HNYIRRY   D HF+
Sbjct: 220 NYPFNKQVKIVIATMAIHNYIRRYSERDRHFD 251

BLAST of Lcy13g012160 vs. NCBI nr
Match: XP_020419135.1 (uncharacterized protein LOC109949131 [Prunus persica] >XP_020419136.1 uncharacterized protein LOC109949131 [Prunus persica])

HSP 1 Score: 165.6 bits (418), Expect = 2.0e-37
Identity = 73/92 (79.35%), Postives = 82/92 (89.13%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M+GYL PYKGERYH PDFRRGSQP GY+E+FN  HSSLRSVIERTFG+WKKRWKILRD+ 
Sbjct: 254 MRGYLGPYKGERYHLPDFRRGSQPTGYKEVFNHVHSSLRSVIERTFGVWKKRWKILRDMP 313

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFE 93
           NYPF KQVKI+IATMTLHNYIRR+ + D HF+
Sbjct: 314 NYPFNKQVKIVIATMTLHNYIRRHAQRDRHFD 345

BLAST of Lcy13g012160 vs. NCBI nr
Match: TXG52401.1 (hypothetical protein EZV62_021570 [Acer yangbiense])

HSP 1 Score: 164.5 bits (415), Expect = 4.5e-37
Identity = 73/93 (78.49%), Postives = 82/93 (88.17%), Query Frame = 0

Query: 1  MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
          M GYL PYKGERYH PDFRRGSQP G+QE+FN AHSSLRSVIERTFG+WKKRWKILRD+ 
Sbjct: 1  MDGYLGPYKGERYHLPDFRRGSQPTGHQEVFNHAHSSLRSVIERTFGVWKKRWKILRDMP 60

Query: 61 NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFEK 94
          +YPF KQVKI+IATM +HNYIRR+ + D HFEK
Sbjct: 61 SYPFDKQVKIVIATMAIHNYIRRHAKHDHHFEK 93

BLAST of Lcy13g012160 vs. NCBI nr
Match: XP_020419156.1 (putative nuclease HARBI1 [Prunus persica])

HSP 1 Score: 163.7 bits (413), Expect = 7.7e-37
Identity = 72/92 (78.26%), Postives = 81/92 (88.04%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M+GYL PYKGERYH PDF RGSQP GY+E+FN  HSSLRSVIERTFG+WKKRWKILRD+ 
Sbjct: 219 MRGYLGPYKGERYHLPDFHRGSQPTGYKEVFNHVHSSLRSVIERTFGVWKKRWKILRDMP 278

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFE 93
           NYPF KQVKI+IATMTLHNYIRR+ + D HF+
Sbjct: 279 NYPFNKQVKIVIATMTLHNYIRRHAQRDRHFD 310

BLAST of Lcy13g012160 vs. NCBI nr
Match: XP_028952690.1 (uncharacterized protein LOC103431050 [Malus domestica])

HSP 1 Score: 161.8 bits (408), Expect = 2.9e-36
Identity = 70/92 (76.09%), Postives = 80/92 (86.96%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M+GYL PYKGERYH PDFRRG +P G++E+FN  HSSLRS+IERTFG+WKKRW ILRD+ 
Sbjct: 306 MRGYLGPYKGERYHLPDFRRGPEPTGHKEVFNHMHSSLRSIIERTFGVWKKRWAILRDMP 365

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFE 93
           NYPF KQVKI+IATMTLHNYIRRY  SD HF+
Sbjct: 366 NYPFNKQVKIVIATMTLHNYIRRYSESDRHFD 397

BLAST of Lcy13g012160 vs. NCBI nr
Match: XP_034202714.1 (putative nuclease HARBI1 [Prunus dulcis])

HSP 1 Score: 159.5 bits (402), Expect = 1.5e-35
Identity = 70/93 (75.27%), Postives = 83/93 (89.25%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           M+GYL PYKGERYH PDFRRG QP+G++E+FN AHSSLRSVIERTFG+WKKRW ILRD+ 
Sbjct: 253 MRGYLGPYKGERYHLPDFRRGVQPMGHKEVFNHAHSSLRSVIERTFGVWKKRWSILRDMP 312

Query: 61  NYPFQKQVKIIIATMTLHNYIRRYDRSDGHFEK 94
           +YPF KQVKI+IATMTLHNYIRR+ + D HF++
Sbjct: 313 SYPFAKQVKIVIATMTLHNYIRRHAKRDCHFDE 345

BLAST of Lcy13g012160 vs. TAIR 10
Match: AT5G35695.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 71.2 bits (173), Expect = 4.9e-13
Identity = 34/86 (39.53%), Postives = 53/86 (61.63%), Query Frame = 0

Query: 4   YLEPYKGERYHPPDFR-RGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDISNY 63
           +L P++G RYH  +F  +   P    E+FN  H SLR+VIER FGI+K R+ I +    +
Sbjct: 62  FLAPFRGVRYHLQEFAGQRRDPETPHELFNLRHVSLRNVIERIFGIFKSRFAIFKSAPPF 121

Query: 64  PFQKQVKIIIATMTLHNYIRRYDRSD 89
            ++KQ  +++    LHN++R+  RSD
Sbjct: 122 SYKKQAGLVLTCAALHNFLRKECRSD 147

BLAST of Lcy13g012160 vs. TAIR 10
Match: AT4G10890.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2439 (InterPro:IPR018838); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 68.9 bits (167), Expect = 2.4e-12
Identity = 30/54 (55.56%), Postives = 38/54 (70.37%), Query Frame = 0

Query: 3   GYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKIL 57
           GYL P++   YH   F RG  PV  QE+FN+ H  LRSVI+RTFG+WK +W+IL
Sbjct: 107 GYLGPHRRILYHLGQFGRGGPPVTVQELFNRKHLDLRSVIDRTFGVWKAKWRIL 160

BLAST of Lcy13g012160 vs. TAIR 10
Match: AT5G41980.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 61.2 bits (147), Expect = 5.0e-10
Identity = 31/82 (37.80%), Postives = 44/82 (53.66%), Query Frame = 0

Query: 1   MKGYLEPYKGERYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKRWKILRDIS 60
           + G++ PY G   +  +  +        E+FN+ H  L   I RTFG  K+R+ IL    
Sbjct: 229 LPGFIAPYHGVSTNSREEAK--------EMFNERHKLLHRAIHRTFGALKERFPILLSAP 288

Query: 61  NYPFQKQVKIIIATMTLHNYIR 83
            YP Q QVK++IA   LHNY+R
Sbjct: 289 PYPLQTQVKLVIAACALHNYVR 302

BLAST of Lcy13g012160 vs. TAIR 10
Match: AT1G43722.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28730.1); Has 924 Blast hits to 912 proteins in 109 species: Archae - 0; Bacteria - 0; Metazoa - 222; Fungi - 31; Plants - 661; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 56.6 bits (135), Expect = 1.2e-08
Identity = 28/56 (50.00%), Postives = 34/56 (60.71%), Query Frame = 0

Query: 2   KGYLEPYKGE-----RYHPPDFRRGSQPVGYQEIFNQAHSSLRSVIERTFGIWKKR 53
           +G L PY+       RYH   F  G +P    E+FNQ H+SLRSVIERTF IWK +
Sbjct: 264 QGLLAPYRSSRNRVVRYHMSQFYYGPRPRNKHELFNQCHTSLRSVIERTFRIWKNK 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5C7H6262.2e-3778.49Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_021570 PE=3 SV=1[more]
M5WCA33.7e-3778.26Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa017281mg PE=3 SV=1[more]
A0A5N5FXF09.2e-3673.91DDE Tnp4 domain-containing protein OS=Pyrus ussuriensis x Pyrus communis OX=2448... [more]
A0A5C7ITA12.1e-3574.19DDE Tnp4 domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000937... [more]
A0A540MIT72.7e-3571.74DDE Tnp4 domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_015721 PE... [more]
Match NameE-valueIdentityDescription
XP_020419135.12.0e-3779.35uncharacterized protein LOC109949131 [Prunus persica] >XP_020419136.1 uncharacte... [more]
TXG52401.14.5e-3778.49hypothetical protein EZV62_021570 [Acer yangbiense][more]
XP_020419156.17.7e-3778.26putative nuclease HARBI1 [Prunus persica][more]
XP_028952690.12.9e-3676.09uncharacterized protein LOC103431050 [Malus domestica][more]
XP_034202714.11.5e-3575.27putative nuclease HARBI1 [Prunus dulcis][more]
Match NameE-valueIdentityDescription
AT5G35695.14.9e-1339.53CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT4G10890.12.4e-1255.56unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2439... [more]
AT5G41980.15.0e-1037.80CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT1G43722.11.2e-0850.00unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 8..79
e-value: 1.5E-10
score: 41.0
NoneNo IPR availablePANTHERPTHR22930:SF212NUCLEASE HARBI1 ISOFORM X1-RELATEDcoord: 1..93
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..93

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g012160.1Lcy13g012160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding