Lcy13g012060 (gene) Sponge gourd (P93075) v1

BLAST of Lcy13g012060 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 6.0e-244
Identity = 423/733 (57.71%), Postives = 549/733 (74.90%), Query Frame = 0

Query: 9   LLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLKESQVEAKE 68
           +L  ++ ++     + E    K  +I+++ D+P  N    ++TH+N+L SL  SQ EAKE
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPINARRK 128
             VYSYTK+FNAFAAKL+  EAK + + E V  V  N+YRKL TT+SWDF+GLP+ A+R 
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 TKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGARYFKL 188
            K E D+I+G+ DTGITP +ESF D G GPPP KWKG+C  + NFTGCNNK+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCW 248
           DG+    ++ SP+DI GHGTHTSS   G  V  ASL G+A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 SGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKAIITVTAA 308
           + +GC+DMD+LA F+AAI DGV++ISISI G    +Y+ D++++G+FHAM+K I+TV +A
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVS 368
           GN GP++G+V NH PWILTVAASGIDR F S  +LGNGK+ SG+GI++F+P  K YPLVS
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYGTIIQSDQF 428
           G D A N   K  A +C   +LD  KVK K++ C++   G +S +KS G  G II SDQ+
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFFSSRGPNPG 488
           L N  +FMAPA+ V+S VG+ I  YI+STR+ +AVI K+RQ+   AP VA FSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 548
           S R+LKPDIAAPG+DILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA  AAYVKS
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 FHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQF 608
           FHP W+PAAI+SA++T+A PISRR+N D EF+YG G ++P RA +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQPTTAVFWRQVTN 668
           LC EGY  ++++ L GT+S+ CS++    G+DSLNYPT QL+L++ +  T AVF R+VTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAKMVSGSLTWA 728
           VG P SVY ATVRAP GVEITV P +LS S+  QK++FKV+VKA  +   K+VSG L W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARYVVRSPIVIY 741
             R+ VRSPIVIY
Sbjct: 734 SPRHSVRSPIVIY 744

BLAST of Lcy13g012060 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.6e-175
Identity = 362/769 (47.07%), Postives = 487/769 (63.33%), Query Frame = 0

Query: 3   ISKSSPLLFFLLFFVVGYVVAV---EINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSL 62
           +S     LF L F +V   V     + N  +K +IV+M +    + V A E H N+LM++
Sbjct: 2   VSNQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTV 61

Query: 63  KESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFI 122
              + +A+E  +YSY K+ N F A+L   EA+ LS+ EGV  V  N  R+L TTRSWDF+
Sbjct: 62  IGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFL 121

Query: 123 GL-PINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNN 182
           GL     +R    ES+IIVG+ DTGI   + SF D G GPPP KWKG C+   NFT CNN
Sbjct: 122 GLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNN 181

Query: 183 KLIGARYFKL--DGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGV 242
           K+IGA+YF +  +G PD  +  +  D  GHGTHTSS   G +V  ASL G+A GTARGGV
Sbjct: 182 KVIGAKYFHIQSEGLPDG-EGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGV 241

Query: 243 PSARVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFH 302
           PSAR+A YKVCW  +GC+DMD+LAAFD AI DGVD+ISISI G     + ED +AIGAFH
Sbjct: 242 PSARIAAYKVCWD-SGCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFH 301

Query: 303 AMKKAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINL 362
           AMK+ I+T  +AGN GP   +V N APW++TVAA+ +DRKF +  +LGNG   SG+ +N 
Sbjct: 302 AMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNG 361

Query: 363 FNPNQKMYPLVSGGDVA-MNPQSKDSASFCVDGTLDPSKVKRKLVFCKLIT-------WG 422
           FNP +KMYPL SG   + ++       S C  GTL   KV  K+V+C+           G
Sbjct: 362 FNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQG 421

Query: 423 ADSVVKSIGAYGTIIQSDQFLPNTDLFMAPASLVSSLV-----GETIDNYIHSTRTPTAV 482
            D VV+S+   G I+   Q L  TD  MA ++L++        G  I  YI+ST+ P AV
Sbjct: 422 QDHVVRSLKGAGVIV---QLLEPTD--MATSTLIAGSYVFFEDGTKITEYINSTKNPQAV 481

Query: 483 IYKSRQLKSAAPIVAFFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQF 542
           I+K++  K  AP ++ FS+RGP   S  ILKPDI+APG++ILA Y+ L S+TG   D + 
Sbjct: 482 IFKTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRR 541

Query: 543 SKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGA 602
           + F++MSGTSMACPH AAAAAYVKSFHP WSPAAI+SAL+TTA+P+  + N + E SYG+
Sbjct: 542 TLFSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGS 601

Query: 603 GNLSPSRARNPGLIYDLNEMSYIQFLCSEGYTGSSISILTG-------TKSIDCSTL-RA 662
           G ++P RA +PGL+YD+ E +Y++FLC EGY  +SI +LTG        K  +C  + R 
Sbjct: 602 GQINPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRG 661

Query: 663 QGYDSLNYPTFQLSLKTTRQPTTAVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLS 722
            G D LNYP+    + +T    + VF+R VTNVG   S Y A V AP G+ + VVP  +S
Sbjct: 662 LGSDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMS 721

Query: 723 LSQLLQKQNFKVIVKASPLPSAK-MVSGSLTWAGAR-YVVRSPIVIYGS 743
             +  +K+NFKV++      + K +VS S+ W  +R ++VRSPI+++ S
Sbjct: 722 FERPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFRS 761

BLAST of Lcy13g012060 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 7.5e-162
Identity = 318/741 (42.91%), Postives = 465/741 (62.75%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVAVEI-NEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSL 60
           MS S    L FF LFF       ++  ++GK  +IV+M  +  L + ++   H   ++  
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRK--LEDPDSAHLHHRAMLEQ 60

Query: 61  KESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFI 120
                 A ES++++Y +SFN FA KLTEEEA+ ++  EGV  V  N   +L TTRSWDF+
Sbjct: 61  VVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFL 120

Query: 121 GLPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNK 180
           G P+   R+++ ES+I+VG+ DTGI P + SF D+GF PPP KWKGTC    NF  CN K
Sbjct: 121 GFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRK 180

Query: 181 LIGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSA 240
           +IGAR + +     P D+  P D +GHGTHT+S A G  V  A+L GL  GTARGGVP A
Sbjct: 181 IIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLA 240

Query: 241 RVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMK 300
           R+A YKVCW+  GCSD D+LAA+D AI DGVD+IS+S+ G    +Y  DA+AIG+FHA++
Sbjct: 241 RIAAYKVCWN-DGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVE 300

Query: 301 KAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNP 360
           + I+T  +AGN GP   +  + +PW+L+VAAS +DRKFV+  ++GNG++  GV IN F  
Sbjct: 301 RGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTF-- 360

Query: 361 NQKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAY 420
           + + YPLVSG D+      K ++ FC D +++P+ +K K+V C+  ++G     KS+   
Sbjct: 361 DNQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCE-ASFGPHEFFKSLDGA 420

Query: 421 GTIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQ-LKSAAPIVA 480
             ++ +       D +  P+S++          YI+S R+P A I+KS   L ++AP+V 
Sbjct: 421 AGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVV 480

Query: 481 FFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPH 540
            FSSRGPN  +  ++KPDI+ PGV+ILA +  +  + G++ +T F+   ++SGTSM+CPH
Sbjct: 481 SFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFN---IISGTSMSCPH 540

Query: 541 VAAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIY 600
           +   A YVK+++P WSPAAI+SAL+TTASP++ R NP  EF+YG+G+++P +A  PGL+Y
Sbjct: 541 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 600

Query: 601 DLNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRAQGYDSLNYPTFQLSLKTTRQPTT 660
           D NE  Y++FLC +GY   ++  +TG  S   S    + +D LNYP+F LS+  + Q   
Sbjct: 601 DANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWD-LNYPSFGLSVSPS-QTFN 660

Query: 661 AVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAK 720
             F R +T+V    S Y A + AP G+ I+V P  LS + L  +++F + V+ S      
Sbjct: 661 QYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGF 720

Query: 721 MVSGSLTWAGARYVVRSPIVI 740
           +VS SL W+   + VRSPI I
Sbjct: 721 VVSASLVWSDGVHYVRSPITI 727

BLAST of Lcy13g012060 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 6.1e-156
Identity = 321/740 (43.38%), Postives = 462/740 (62.43%), Query Frame = 0

Query: 6   SSPL-LFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLKESQV 65
           S+PL L  L F     V A +  +    +IV+M   P + + +    HL++L  L    +
Sbjct: 5   STPLYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEI-KYSPPSHHLSILQKL-VGTI 64

Query: 66  EAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPIN 125
            A   +V SY +SFN FAA L++ E++ L   + V  V P++  +L TTRSWDF+G    
Sbjct: 65  AASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEK 124

Query: 126 ARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGAR 185
           ARR++ +ESD+IVG+ D+GI P +ESF D+GFGPPP+KWKG+C     F  CNNKLIGAR
Sbjct: 125 ARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGAR 184

Query: 186 YFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMY 245
           ++            S  D  GHGTHT+S A GNAV  AS  GLA GTARGGVPSAR+A Y
Sbjct: 185 FYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAY 244

Query: 246 KVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKAIIT 305
           KVC++   C+D+D+LAAFD AI DGVDVISISI+ +   N    +VAIG+FHAM + IIT
Sbjct: 245 KVCFN--RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIIT 304

Query: 306 VTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMY 365
             +AGN GP  GSV N +PW++TVAASG DR+F+    LGNGK ++G+ +N FN N   +
Sbjct: 305 AGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKF 364

Query: 366 PLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYGTIIQ 425
           P+V G +V+ N  S+  A +C  G +D   VK K+V C       ++ +   GA G I+Q
Sbjct: 365 PIVYGQNVSRN-CSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQ 424

Query: 426 SDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQL-KSAAPIVAFFSSR 485
           +     +  +   PAS +     ++I +YI S   P A I ++ ++    AP V  FSSR
Sbjct: 425 NTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSR 484

Query: 486 GPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKG--DTQFSKFTLMSGTSMACPHVAA 545
           GP+     +LKPD++APG++ILA ++P+ S +      D +  ++++MSGTSMACPHVA 
Sbjct: 485 GPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAG 544

Query: 546 AAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYDLN 605
            AAYVKSFHP WSP+AI+SA++TTA+P++ + NP+ EF+YG+G ++P++A +PGL+Y++ 
Sbjct: 545 VAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVE 604

Query: 606 EMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRAQGYDSLNYPTFQLSLKTTRQPTTAVF 665
              Y++ LC+EG+  ++++  +G +++ CS  R +  D LNYPT   +  ++  P    F
Sbjct: 605 TEDYLKMLCAEGFDSTTLTTTSG-QNVTCSE-RTEVKD-LNYPT-MTTFVSSLDPFNVTF 664

Query: 666 WRQVTNVGRPVSVYNAT-VRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAKMV 725
            R VTNVG P S Y A+ V   P ++I++ P  L    L +K++F V +    L     V
Sbjct: 665 KRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFV 724

Query: 726 SGSLTWAGARYVVRSPIVIY 741
           S S+ W+   + VRSPIV Y
Sbjct: 725 SSSVVWSDGSHSVRSPIVAY 725

BLAST of Lcy13g012060 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 8.8e-155
Identity = 323/742 (43.53%), Postives = 459/742 (61.86%), Query Frame = 0

Query: 10  LFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLK-ESQVEAKE 69
           +F LL  VV +  A + ++ K+ +IV+M   P   +   +  H ++L  +  ES ++  +
Sbjct: 13  IFALL--VVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQ--D 72

Query: 70  SMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPINARRK 129
            +V +Y +SFN FAA+LTE E + L+  + V  V P++   LQTT SW+F+GL    R K
Sbjct: 73  RLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTK 132

Query: 130 TKQ--ESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGARYF 189
                ESD I+G+ D+GI P ++SF   GFGPPP+KWKG C    NFT CNNKLIGARY+
Sbjct: 133 RNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARYY 192

Query: 190 --KLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMY 249
             KL+G P+     S  D +GHG+HT+SIA GNAV   S  GL  GT RGGVP+AR+A+Y
Sbjct: 193 TPKLEGFPE-----SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVY 252

Query: 250 KVCWSGT-GCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKAII 309
           KVC  G   C+   +LAAFD AI D VD+I++S+  +  G + ED +AIGAFHAM K I+
Sbjct: 253 KVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGIL 312

Query: 310 TVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKM 369
           TV  AGN GP   ++V+ APW+ TVAAS ++R F++   LGNGK I G  +N F+ N K 
Sbjct: 313 TVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKK 372

Query: 370 YPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYGTII 429
           YPLV G   A +     SA FC  G LD  +VK K+V C   T       +++GA  +I+
Sbjct: 373 YPLVYGKS-ASSRCDASSAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASIV 432

Query: 430 QSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQL-KSAAPIVAFFSS 489
           + + +     +F  P S++S      + +Y++ST+ P A + KS  +    AP+VA +SS
Sbjct: 433 R-NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSS 492

Query: 490 RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 549
           RGPNP  + ILKPDI APG +ILA Y+P   +   + DT+  K+T++SGTSM+CPHVA  
Sbjct: 493 RGPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGV 552

Query: 550 AAYVKSFHPLWSPAAIRSALLTTASPISRRLNPD---GEFSYGAGNLSPSRARNPGLIYD 609
           AAY+K+FHPLWSP+ I+SA++TTA P++   +P     EF+YGAG++ P  A +PGL+Y+
Sbjct: 553 AAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYE 612

Query: 610 LNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRAQGYD-SLNYPTFQLSLKTTRQPTT 669
            N+  +I FLC   YTG  + +++G  S  C+  + +    +LNYP+    +  T+ P  
Sbjct: 613 ANKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGTK-PFK 672

Query: 670 AVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAK 729
             F R VTNVGRP + Y A V     +++ VVPA LSL  L +K++F V V  +   +  
Sbjct: 673 VTFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAEN 732

Query: 730 MVSGSLTWAGARYVVRSPIVIY 741
           +VS  L W+   + VRSPIV+Y
Sbjct: 733 LVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lcy13g012060 vs. ExPASy TrEMBL
Match: A0A0A0KME6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G000010 PE=3 SV=1)

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 595/741 (80.30%), Postives = 666/741 (89.88%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLK 60
           MSISKSS LL F LF VVG V  ++ +E K H+IVF+E++PVLNEV+ VETHLN+LMS+K
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIG 120
           +S  EA ESMVYSYTKSFNAFAAKL+++EAK LS R+ V HVIPN+YRKLQTTRSWDFIG
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 LPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKL 180
           L  NARR TK ESDIIVGLFDTGITPTA+SFKDDGFGPPP+KWKGTC HFANFT CN KL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSAR 240
           IGARYFKLDG+PDP DILSPVD  GHGTHTSS ATGNA+ GASLSGLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKK 300
           VAMYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHAMKK
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 AIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPN 360
            IITVT+AGN GPTAGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP 
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 QKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYG 420
           QKMYPLVSGGDVA N +SKD+ASFC++GTLDP+KVK  LVFCKL+TWGADSV+KSIGA G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 TIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFF 480
            IIQSD+FL N D+FMAPA++VSSLVG  I  YI STRTPTAVIYK++QLK+ AP+VA F
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYDL 600
           AAAAYVKSFHPLWSPAAIRSALLTTA+PISRRLNP+GEF+YGAGNL+PSRA +PGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQPTTA 660
           NE+SYIQFLCSEGYTGSSI++L+GTKSI+CS L   QG+DSLNYPTFQLSLK+T QP T 
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAKM 720
            F R+VTNVG P+SVYNAT+ APPGV ITV P TLS S+LLQK++FKV+VKASPLPSAKM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLTWAGARYVVRSPIVIY 741
           VSGSL W GA++VVRSPIV+Y
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVY 741

BLAST of Lcy13g012060 vs. ExPASy TrEMBL
Match: A0A1S3BZ36 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495168 PE=3 SV=1)

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 588/713 (82.47%), Postives = 649/713 (91.02%), Query Frame = 0

Query: 30  KKHFIVFMED-QPVLNEVNAVETHLNVLMSLKESQVEAKESMVYSYTKSFNAFAAKLTEE 89
           +KHFIVF+E+ + +LNEV+ ++ +LNVLMS+KES VEAKESMVYSYT +FNAFAAKLTE 
Sbjct: 61  QKHFIVFLENGRTILNEVDGLDINLNVLMSVKESHVEAKESMVYSYTNNFNAFAAKLTEA 120

Query: 90  EAKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPINARRKTKQESDIIVGLFDTGITPTA 149
           EAKTLS+R  V HVIPNRYRKLQTTRSWDF+G PINA+RKT+QESDIIVGLFDTGITPTA
Sbjct: 121 EAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPINAKRKTRQESDIIVGLFDTGITPTA 180

Query: 150 ESFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGARYFKLDGSPDPLDILSPVDISGHGT 209
           +SFKDDGFGPPP+KWKGTC HFANF+GCNNKLIGARYFKLDG  +PLDILSP+DI+GHGT
Sbjct: 181 DSFKDDGFGPPPKKWKGTCDHFANFSGCNNKLIGARYFKLDGLTEPLDILSPIDINGHGT 240

Query: 210 HTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSDMDLLAAFDAAIQD 269
           HTSS ATGN + GA+LSGLA GTARGGVPSARVAMYKVCW+ TGCSDMDLLAAFDAAIQD
Sbjct: 241 HTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCSDMDLLAAFDAAIQD 300

Query: 270 GVDVISISIAGNGFGNYTEDAVAIGAFHAMKKAIITVTAAGNTGPTAGSVVNHAPWILTV 329
           GVDVISISIAG G+GNYTED ++IGAFHAMKK IITVTAAGN GP+AG+VVNHAPWILTV
Sbjct: 301 GVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIITVTAAGNNGPSAGTVVNHAPWILTV 360

Query: 330 AASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMNPQSKDSASFCVDG 389
           AAS IDR F+SP ELGNGKNISGVGINLFNP +KMY LVSG DVA N +SKD A++CV+ 
Sbjct: 361 AASSIDRTFISPVELGNGKNISGVGINLFNPEKKMYKLVSGEDVAKNIESKDDATYCVEN 420

Query: 390 TLDPSKVKRKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLFMAPASLVSSLVGE 449
           +LDPSKVK  +VFCKL+TWGADS VKSIGA G I+QSDQFL NTD+FMAP++LVSS VG 
Sbjct: 421 SLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDIFMAPSTLVSSFVGA 480

Query: 450 TIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILKPDIAAPGVDILAGY 509
           TID+YIHS+RTPTAVIYK+RQ   AAPIVA FSSRGPNPGS RILKPDIAAPGV+ILAGY
Sbjct: 481 TIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILKPDIAAPGVNILAGY 540

Query: 510 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTASP 569
           TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTA P
Sbjct: 541 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 600

Query: 570 ISRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGYTGSSISILTGTKSI 629
           ISRR NPDGEF YGAGNL+P +A+NPGLIYDLNEMSYIQFLCSEGY+GSSISILTGTKSI
Sbjct: 601 ISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGYSGSSISILTGTKSI 660

Query: 630 DCST-LRAQGYDSLNYPTFQLSLKTTRQPTTAVFWRQVTNVGRPVSVYNATVRAPPGVEI 689
           +C+T +R QGYDSLNYPTFQL L++TRQPTTAVFWR+VTNVG PVSVYN TV APPGVEI
Sbjct: 661 NCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVSVYNTTVWAPPGVEI 720

Query: 690 TVVPATLSLSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAGARYVVRSPIVIY 741
           TV PATLS S LLQK+ FKV+VKA+PLPS KMVSGS+TW   RYVVRSPIV+Y
Sbjct: 721 TVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVVRSPIVVY 773

BLAST of Lcy13g012060 vs. ExPASy TrEMBL
Match: A0A5A7UKY8 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002590 PE=3 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 590/744 (79.30%), Postives = 663/744 (89.11%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVA---VEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLM 60
           MS+SKSS LL F LF +VG VV     + NE K HFIVF++++PVLNEV+AVETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SLKESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWD 120
           S+K+S  EA ESMVYSYTKSFNAFAAKL++ EAK LS R+ V HVIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCN 180
           FIGL  NARR+TK ESDIIVGLFDTGITPTA+SF+DDGFGPPP+KWKGTC HFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVP 240
            KLIGARYFKLDG+PDP DILSPVD  GHGTHTSS ATGNAV GASLSGLA GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHA 300
           SARVAMYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLF 360
           MKK IITVT+AGN GP AGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPNQKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIG 420
           NP +KMYPLVSGGDVA N +SKD+ASFC++ +LDP+KVK  LVFCKL+TWGADSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 AYGTIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIV 480
           A G IIQSDQFL N D+FMAPA++VSS VG  I  YI STRTPTAVIYK+RQLK+ AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 AFFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           A FSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLI 600
           HVAAAAAYVKSFHPLWSPAAIRSALLTT  PISRRLNP+GEF+YGAGNL+PS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQP 660
           YDLNEMSYIQFLCSEGYTGSSI++L GTKSI+CSTL    G+DSLNYPTFQLSL++T+QP
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 TTAVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPS 720
            T  F RQVTNVG+PVSVYNAT++APPGV+ITV P TLS S+LLQK++FKV+VKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 AKMVSGSLTWAGARYVVRSPIVIY 741
           AKMVSGSL W G R+VVRSPIV+Y
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVY 744

BLAST of Lcy13g012060 vs. ExPASy TrEMBL
Match: A0A1S3C036 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495082 PE=3 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 590/744 (79.30%), Postives = 663/744 (89.11%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVA---VEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLM 60
           MS+SKSS LL F LF +VG VV     + NE K HFIVF++++PVLNEV+AVETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SLKESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWD 120
           S+K+S  EA ESMVYSYTKSFNAFAAKL++ EAK LS R+ V HVIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCN 180
           FIGL  NARR+TK ESDIIVGLFDTGITPTA+SF+DDGFGPPP+KWKGTC HFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVP 240
            KLIGARYFKLDG+PDP DILSPVD  GHGTHTSS ATGNAV GASLSGLA GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHA 300
           SARVAMYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLF 360
           MKK IITVT+AGN GP AGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPNQKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIG 420
           NP +KMYPLVSGGDVA N +SKD+ASFC++ +LDP+KVK  LVFCKL+TWGADSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 AYGTIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIV 480
           A G IIQSDQFL N D+FMAPA++VSS VG  I  YI STRTPTAVIYK+RQLK+ AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 AFFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           A FSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLI 600
           HVAAAAAYVKSFHPLWSPAAIRSALLTT  PISRRLNP+GEF+YGAGNL+PS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQP 660
           YDLNEMSYIQFLCSEGYTGSSI++L GTKSI+CSTL    G+DSLNYPTFQLSL++T+QP
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 TTAVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPS 720
            T  F RQVTNVG+PVSVYNAT++APPGV+ITV P TLS S+LLQK++FKV+VKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 AKMVSGSLTWAGARYVVRSPIVIY 741
           AKMVSGSL W G R+VVRSPIV+Y
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVY 744

BLAST of Lcy13g012060 vs. ExPASy TrEMBL
Match: A0A6J1FLU9 (subtilisin-like protease SBT4.14 OS=Cucurbita moschata OX=3662 GN=LOC111446986 PE=3 SV=1)

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 577/741 (77.87%), Postives = 648/741 (87.45%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLK 60
           M IS SS +L  +L FV+G V AVEI+E KKHFIVF+E +P LNE +AVETHLNVLMS+K
Sbjct: 1   MLISISSRVLVCVLLFVIGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIG 120
           ES VEA+ESMVYSYTKSFNAFAAKLTE+EA  LSKRE V HVIPNRYRKLQTTRSWDFI 
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIE 120

Query: 121 LPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKL 180
           LP  ARR +K E+DIIVGLFDTGITPTA+SFKDDGFGPPP+KWKGTC HFANFTGCN KL
Sbjct: 121 LPSKARRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNQKL 180

Query: 181 IGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSAR 240
           IGARYFKLDG+PDP DILSP+D+ GHGTHTSS ATGNAV GASLSGLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKK 300
           VAMYKVCW+ TGC+DMD+LAAFDAAI DGVDVISISI G  FGNY++D+++IGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 AIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPN 360
            IITVT+AGN GPT GSVVNHAPWI+TV AS IDRKF+S  ELGNGKNISGVGIN FNP 
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINTFNPK 360

Query: 361 QKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYG 420
           +KMYPLV GGDVA   ++++SAS C + +LDPSKVK  LVFC+LITWG DSVV ++GA G
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 TIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFF 480
            IIQSD++L N ++FMAPA++VSS VG  I +YI STRTPTAVIYK+RQLK+AAP+ A F
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYDL 600
           AAAAYVKSFHPLWSP AIRSAL+TTAS ISR+LNPDGEF+YGAGNL+PSRA NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASRISRQLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQPTTA 660
           NEMSYIQFLCSEGYTGSSI +L GTKSI+CSTL    G+DSLNYPTFQL LK+ ++  + 
Sbjct: 601 NEMSYIQFLCSEGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPQRAMST 660

Query: 661 VFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAKM 720
           VF R+VTNVG PVSVYNAT++APPGVEITV P+TLS SQL QK++FKV VKA PL S KM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSQLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSLTWAGARYVVRSPIVIY 741
           VSGS+ W GAR+ VRSPIV+Y
Sbjct: 721 VSGSVAWIGARHAVRSPIVVY 740

BLAST of Lcy13g012060 vs. NCBI nr
Match: XP_038892956.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 593/741 (80.03%), Postives = 666/741 (89.88%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLK 60
           MSISKSS LL F LFF+V  V A+++++ K HFIVF+E++ VLNEV+ VETHLNVLMS+K
Sbjct: 1   MSISKSSRLLVFALFFIVVCVAALDVDKEKNHFIVFLENKSVLNEVDGVETHLNVLMSVK 60

Query: 61  ESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIG 120
           +S  EAKESMVYSY+KSFNAFAAKLTE+EAK LS RE V HVIPN+YRKLQTTRSWDF+G
Sbjct: 61  KSHAEAKESMVYSYSKSFNAFAAKLTEDEAKKLSTREDVHHVIPNKYRKLQTTRSWDFLG 120

Query: 121 LPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKL 180
           L  NARR TK ESDI+VGLFDTGITPTA+SFKDDGFGPPP+KWKGTC H+ANFTGCNNKL
Sbjct: 121 LSSNARRNTKHESDIVVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHYANFTGCNNKL 180

Query: 181 IGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSAR 240
           IGARYFKLDGSPDP DILSPVD  GHGTHTSS ATGNAV GASLSGLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGSPDPADILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKK 300
           V+MYKVCW+ +GCSDMD+LAAFDAAI DGVDVISISI G GF NY+ED+++IGAFHAMKK
Sbjct: 241 VSMYKVCWASSGCSDMDILAAFDAAIHDGVDVISISIGGGGFNNYSEDSISIGAFHAMKK 300

Query: 301 AIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPN 360
            IITVT+AGN+GP AGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP 
Sbjct: 301 GIITVTSAGNSGPLAGSVVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 QKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYG 420
           QKMYPLVSGGDVA N +SKDSAS+C + +LDP+KVK  LVFCKL+TWGADSV+KS+GA G
Sbjct: 361 QKMYPLVSGGDVARNSESKDSASYCEEDSLDPNKVKGNLVFCKLMTWGADSVIKSLGANG 420

Query: 421 TIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFF 480
            IIQSDQFL N D+FMAPA++VSS +G  I  YI STRTPTAVIYK++QLK+ AP+VA F
Sbjct: 421 VIIQSDQFLDNADIFMAPATMVSSSLGTIIHTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPGSNRILKPDIAAPGVDILA YTPLKSLTGLKGDTQ+SKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGSNRILKPDIAAPGVDILASYTPLKSLTGLKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYDL 600
           AAAAYVKSFHP WSPAAIRSAL+TTA PISRRLNPDGEF+YGAGNL+PSRA +PGLIYD+
Sbjct: 541 AAAAYVKSFHPHWSPAAIRSALITTARPISRRLNPDGEFAYGAGNLNPSRAISPGLIYDV 600

Query: 601 NEMSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQPTTA 660
           NE+SY+QFLCSEGYTGSSI++L GTKSI+CS L   QG+DSLNYPTFQLSLK+TRQPTT 
Sbjct: 601 NEISYVQFLCSEGYTGSSIAVLAGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTRQPTTT 660

Query: 661 VFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAKM 720
            F R+VTNV  PVSV+NAT++APPGVEITV P TLS S+LLQK++FKV+VKASPLPS KM
Sbjct: 661 TFRRRVTNVAHPVSVFNATIKAPPGVEITVTPTTLSFSRLLQKRSFKVVVKASPLPSTKM 720

Query: 721 VSGSLTWAGARYVVRSPIVIY 741
           VSGS+ W GAR+VVRSPIV+Y
Sbjct: 721 VSGSIAWIGARHVVRSPIVVY 741

BLAST of Lcy13g012060 vs. NCBI nr
Match: XP_011654432.1 (subtilisin-like protease SBT4.14 [Cucumis sativus] >KGN49552.1 hypothetical protein Csa_018364 [Cucumis sativus])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 595/741 (80.30%), Postives = 666/741 (89.88%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLK 60
           MSISKSS LL F LF VVG V  ++ +E K H+IVF+E++PVLNEV+ VETHLN+LMS+K
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIG 120
           +S  EA ESMVYSYTKSFNAFAAKL+++EAK LS R+ V HVIPN+YRKLQTTRSWDFIG
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 LPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKL 180
           L  NARR TK ESDIIVGLFDTGITPTA+SFKDDGFGPPP+KWKGTC HFANFT CN KL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSAR 240
           IGARYFKLDG+PDP DILSPVD  GHGTHTSS ATGNA+ GASLSGLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKK 300
           VAMYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHAMKK
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 AIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPN 360
            IITVT+AGN GPTAGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP 
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 QKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYG 420
           QKMYPLVSGGDVA N +SKD+ASFC++GTLDP+KVK  LVFCKL+TWGADSV+KSIGA G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 TIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFF 480
            IIQSD+FL N D+FMAPA++VSSLVG  I  YI STRTPTAVIYK++QLK+ AP+VA F
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYDL 600
           AAAAYVKSFHPLWSPAAIRSALLTTA+PISRRLNP+GEF+YGAGNL+PSRA +PGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQPTTA 660
           NE+SYIQFLCSEGYTGSSI++L+GTKSI+CS L   QG+DSLNYPTFQLSLK+T QP T 
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAKM 720
            F R+VTNVG P+SVYNAT+ APPGV ITV P TLS S+LLQK++FKV+VKASPLPSAKM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLTWAGARYVVRSPIVIY 741
           VSGSL W GA++VVRSPIV+Y
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVY 741

BLAST of Lcy13g012060 vs. NCBI nr
Match: XP_038891596.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 582/712 (81.74%), Postives = 654/712 (91.85%), Query Frame = 0

Query: 31   KHFIVFMEDQP-VLNEVNAVETHLNVLMSLKESQVEAKESMVYSYTKSFNAFAAKLTEEE 90
            KHFIVF++D+P +LNEV+A+E++LNVLMS+KES VEAK+SMVYSYT +FNAFAAKL EEE
Sbjct: 381  KHFIVFLKDRPAILNEVDAIESNLNVLMSVKESNVEAKKSMVYSYTNNFNAFAAKLNEEE 440

Query: 91   AKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPINARRKTKQESDIIVGLFDTGITPTAE 150
            AK LS+R  V+HVIPN+YRKLQTTRSWDF+GL  NARR TK ESDI+VGLFDTGITPTA+
Sbjct: 441  AKRLSERGDVNHVIPNKYRKLQTTRSWDFLGLSSNARRNTKHESDIVVGLFDTGITPTAD 500

Query: 151  SFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGARYFKLDGSPDPLDILSPVDISGHGTH 210
            SFKDDGFGPPP+KWKGTCLHFANFTGCNNKLIGARYFKLDG PDPLDILSPVD++GHGTH
Sbjct: 501  SFKDDGFGPPPKKWKGTCLHFANFTGCNNKLIGARYFKLDGLPDPLDILSPVDVNGHGTH 560

Query: 211  TSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSDMDLLAAFDAAIQDG 270
            T+S A GN + GA+LSGLA GTARGGVPSARVAMYKVCW+ TGCSDMDLLAAFDAAI+DG
Sbjct: 561  TASTAAGNVIAGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCSDMDLLAAFDAAIRDG 620

Query: 271  VDVISISIAGNGFGNYTEDAVAIGAFHAMKKAIITVTAAGNTGPTAGSVVNHAPWILTVA 330
            VDVISISIAG GFGNYTED ++IGAF AMKK IITV AAGN GP+AG+VVNHAPWI+TVA
Sbjct: 621  VDVISISIAGIGFGNYTEDPISIGAFQAMKKGIITVAAAGNNGPSAGTVVNHAPWIVTVA 680

Query: 331  ASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMNPQSKDSASFCVDGT 390
            AS IDR+F+SP +LGNGKNISGVGINLFNP QKMY LVSGGDVA + +SKDSA FCV+G+
Sbjct: 681  ASSIDRRFISPLQLGNGKNISGVGINLFNPQQKMYKLVSGGDVAKDIESKDSAMFCVEGS 740

Query: 391  LDPSKVKRKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLFMAPASLVSSLVGET 450
            LDP KVK  +VFCKL+TWGADSVVKSIGAYG I+QSDQF+ NTD+FMAP++LVSS VG T
Sbjct: 741  LDPIKVKDSIVFCKLMTWGADSVVKSIGAYGAILQSDQFIDNTDIFMAPSTLVSSFVGLT 800

Query: 451  IDNYIHSTRTPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILKPDIAAPGVDILAGYT 510
            ID+YIHSTRTPTAVIYK+RQL++AAP VA FSSRGPNPGS RILKPDIAAPGV+ILAGYT
Sbjct: 801  IDSYIHSTRTPTAVIYKTRQLRAAAPFVAAFSSRGPNPGSTRILKPDIAAPGVNILAGYT 860

Query: 511  PLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTASPI 570
            PLKSLTGLKGD+QFSKFTLMSGTSMACPHVAAAA YVKSFHPLWSPAAIRSALLTTA+PI
Sbjct: 861  PLKSLTGLKGDSQFSKFTLMSGTSMACPHVAAAATYVKSFHPLWSPAAIRSALLTTATPI 920

Query: 571  SRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGYTGSSISILTGTKSID 630
            SRR+NP+GEF YGAGNL+P++A+NPGLIYDL++MSYIQFLCSEGYTGSSI+ILTGTKS+D
Sbjct: 921  SRRMNPNGEFGYGAGNLNPAKAKNPGLIYDLDDMSYIQFLCSEGYTGSSIAILTGTKSLD 980

Query: 631  CST-LRAQGYDSLNYPTFQLSLKTTRQPTTAVFWRQVTNVGRPVSVYNATVRAPPGVEIT 690
            CST +  QGYDSLNYPTFQL+LK+T+QPTTAVFWR+VTNVGRPVS YNAT+RAPPGVEI 
Sbjct: 981  CSTIIPGQGYDSLNYPTFQLTLKSTQQPTTAVFWREVTNVGRPVSTYNATIRAPPGVEIM 1040

Query: 691  VVPATLSLSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAGARYVVRSPIVIY 741
            VVP TLS SQLLQKQ FKV+VK+ PLPS  MVSG ++W   RYVVRSPIV+Y
Sbjct: 1041 VVPTTLSFSQLLQKQRFKVVVKSGPLPSTNMVSGWISWVDVRYVVRSPIVVY 1092

BLAST of Lcy13g012060 vs. NCBI nr
Match: KAE8648139.1 (hypothetical protein Csa_021522 [Cucumis sativus])

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 590/742 (79.51%), Postives = 664/742 (89.49%), Query Frame = 0

Query: 1   MSISKSSPLLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQP-VLNEVNAVETHLNVLMSL 60
           MSISK S +   +LF++ G V AVEI E KKHFIVFME++P +LNEV+ ++T+LNVLMS+
Sbjct: 1   MSISKFSSVFIIVLFYIAGCVAAVEIVEDKKHFIVFMENRPTILNEVDGLDTNLNVLMSV 60

Query: 61  KESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFI 120
           KES V+AK+ MV+SYT +FNAFAAKLTE EAKTLS+R  V HVIPNRYRKLQTTRSWDF+
Sbjct: 61  KESHVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFL 120

Query: 121 GLPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNK 180
           G PINA+RKT+QESDIIVGLFDTGITPTA+SFKDDG+GPPP+KWKGTC HFANF+GCNNK
Sbjct: 121 GFPINAKRKTRQESDIIVGLFDTGITPTADSFKDDGYGPPPKKWKGTCDHFANFSGCNNK 180

Query: 181 LIGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSA 240
           LIGARYFKLDG  +P DILSPVD++GHGTHTSS ATGN + GA+LSGLA GTA GGVPSA
Sbjct: 181 LIGARYFKLDGITEPFDILSPVDVNGHGTHTSSTATGNVITGANLSGLAQGTAPGGVPSA 240

Query: 241 RVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMK 300
           R+AMYKVCW   GCSDMDLLAAFDAAIQDGVDVISISIAG G+GNYT+D ++IGAFHAMK
Sbjct: 241 RLAMYKVCWMSNGCSDMDLLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMK 300

Query: 301 KAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNP 360
           K IITVTAAGN GP+AG+VVNHAPWILTVAAS IDR+F+SP ELGNGKNISGVGINLFNP
Sbjct: 301 KGIITVTAAGNNGPSAGTVVNHAPWILTVAASSIDRRFISPVELGNGKNISGVGINLFNP 360

Query: 361 NQKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAY 420
            +KMY LVSG DVA N + KD+A +C D +LDPSKVK  LVFCKL+TWGADS VKSIGA 
Sbjct: 361 EKKMYKLVSGEDVAKNIEGKDNAMYCEDKSLDPSKVKDSLVFCKLMTWGADSTVKSIGAA 420

Query: 421 GTIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIVAF 480
           G I+QSDQFL NTD+FMAP++LVSS VG TID YIHSTRTPTAVIYK+RQ ++AAPI+A 
Sbjct: 421 GAILQSDQFLDNTDIFMAPSALVSSFVGATIDAYIHSTRTPTAVIYKTRQHRAAAPIIAP 480

Query: 481 FSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHV 540
           FSSRGPNPGS  ILKPDIAAPGV+ILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHV
Sbjct: 481 FSSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHV 540

Query: 541 AAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYD 600
           AAAAAYVKSFHPLWSPAAIRSALLTTA PISRR NPDGEF YGAGNL+P +A+NPGLIYD
Sbjct: 541 AAAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPRKAKNPGLIYD 600

Query: 601 LNEMSYIQFLCSEGYTGSSISILTGTKSIDCST-LRAQGYDSLNYPTFQLSLKTTRQPTT 660
           LNEMSYIQFLC EGY+GSSI ILTGTKSI+C+T +  +GYDSLNYPTFQLSL+++R+PTT
Sbjct: 601 LNEMSYIQFLCREGYSGSSIIILTGTKSINCATIIPGEGYDSLNYPTFQLSLQSSREPTT 660

Query: 661 AVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAK 720
           AVFWR+VTNVG+PVSVYNATVRAPPGVEITV PATLS S L QK+ FKV+VKA+PLP+ K
Sbjct: 661 AVFWREVTNVGKPVSVYNATVRAPPGVEITVEPATLSFSYLHQKERFKVVVKANPLPANK 720

Query: 721 MVSGSLTWAGARYVVRSPIVIY 741
           MVSGS+TW   RYVVRSP+V+Y
Sbjct: 721 MVSGSITWFDPRYVVRSPVVVY 742

BLAST of Lcy13g012060 vs. NCBI nr
Match: XP_008454861.1 (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis melo])

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 588/713 (82.47%), Postives = 649/713 (91.02%), Query Frame = 0

Query: 30  KKHFIVFMED-QPVLNEVNAVETHLNVLMSLKESQVEAKESMVYSYTKSFNAFAAKLTEE 89
           +KHFIVF+E+ + +LNEV+ ++ +LNVLMS+KES VEAKESMVYSYT +FNAFAAKLTE 
Sbjct: 61  QKHFIVFLENGRTILNEVDGLDINLNVLMSVKESHVEAKESMVYSYTNNFNAFAAKLTEA 120

Query: 90  EAKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPINARRKTKQESDIIVGLFDTGITPTA 149
           EAKTLS+R  V HVIPNRYRKLQTTRSWDF+G PINA+RKT+QESDIIVGLFDTGITPTA
Sbjct: 121 EAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPINAKRKTRQESDIIVGLFDTGITPTA 180

Query: 150 ESFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGARYFKLDGSPDPLDILSPVDISGHGT 209
           +SFKDDGFGPPP+KWKGTC HFANF+GCNNKLIGARYFKLDG  +PLDILSP+DI+GHGT
Sbjct: 181 DSFKDDGFGPPPKKWKGTCDHFANFSGCNNKLIGARYFKLDGLTEPLDILSPIDINGHGT 240

Query: 210 HTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSDMDLLAAFDAAIQD 269
           HTSS ATGN + GA+LSGLA GTARGGVPSARVAMYKVCW+ TGCSDMDLLAAFDAAIQD
Sbjct: 241 HTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCSDMDLLAAFDAAIQD 300

Query: 270 GVDVISISIAGNGFGNYTEDAVAIGAFHAMKKAIITVTAAGNTGPTAGSVVNHAPWILTV 329
           GVDVISISIAG G+GNYTED ++IGAFHAMKK IITVTAAGN GP+AG+VVNHAPWILTV
Sbjct: 301 GVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIITVTAAGNNGPSAGTVVNHAPWILTV 360

Query: 330 AASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMNPQSKDSASFCVDG 389
           AAS IDR F+SP ELGNGKNISGVGINLFNP +KMY LVSG DVA N +SKD A++CV+ 
Sbjct: 361 AASSIDRTFISPVELGNGKNISGVGINLFNPEKKMYKLVSGEDVAKNIESKDDATYCVEN 420

Query: 390 TLDPSKVKRKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLFMAPASLVSSLVGE 449
           +LDPSKVK  +VFCKL+TWGADS VKSIGA G I+QSDQFL NTD+FMAP++LVSS VG 
Sbjct: 421 SLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDIFMAPSTLVSSFVGA 480

Query: 450 TIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILKPDIAAPGVDILAGY 509
           TID+YIHS+RTPTAVIYK+RQ   AAPIVA FSSRGPNPGS RILKPDIAAPGV+ILAGY
Sbjct: 481 TIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILKPDIAAPGVNILAGY 540

Query: 510 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTASP 569
           TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTA P
Sbjct: 541 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 600

Query: 570 ISRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGYTGSSISILTGTKSI 629
           ISRR NPDGEF YGAGNL+P +A+NPGLIYDLNEMSYIQFLCSEGY+GSSISILTGTKSI
Sbjct: 601 ISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGYSGSSISILTGTKSI 660

Query: 630 DCST-LRAQGYDSLNYPTFQLSLKTTRQPTTAVFWRQVTNVGRPVSVYNATVRAPPGVEI 689
           +C+T +R QGYDSLNYPTFQL L++TRQPTTAVFWR+VTNVG PVSVYN TV APPGVEI
Sbjct: 661 NCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVSVYNTTVWAPPGVEI 720

Query: 690 TVVPATLSLSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAGARYVVRSPIVIY 741
           TV PATLS S LLQK+ FKV+VKA+PLPS KMVSGS+TW   RYVVRSPIV+Y
Sbjct: 721 TVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVVRSPIVVY 773

BLAST of Lcy13g012060 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 845.1 bits (2182), Expect = 4.2e-245
Identity = 423/733 (57.71%), Postives = 549/733 (74.90%), Query Frame = 0

Query: 9   LLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLKESQVEAKE 68
           +L  ++ ++     + E    K  +I+++ D+P  N    ++TH+N+L SL  SQ EAKE
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPINARRK 128
             VYSYTK+FNAFAAKL+  EAK + + E V  V  N+YRKL TT+SWDF+GLP+ A+R 
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 TKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGARYFKL 188
            K E D+I+G+ DTGITP +ESF D G GPPP KWKG+C  + NFTGCNNK+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCW 248
           DG+    ++ SP+DI GHGTHTSS   G  V  ASL G+A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 SGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKAIITVTAA 308
           + +GC+DMD+LA F+AAI DGV++ISISI G    +Y+ D++++G+FHAM+K I+TV +A
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVS 368
           GN GP++G+V NH PWILTVAASGIDR F S  +LGNGK+ SG+GI++F+P  K YPLVS
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYGTIIQSDQF 428
           G D A N   K  A +C   +LD  KVK K++ C++   G +S +KS G  G II SDQ+
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQLKSAAPIVAFFSSRGPNPG 488
           L N  +FMAPA+ V+S VG+ I  YI+STR+ +AVI K+RQ+   AP VA FSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 548
           S R+LKPDIAAPG+DILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA  AAYVKS
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 FHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQF 608
           FHP W+PAAI+SA++T+A PISRR+N D EF+YG G ++P RA +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCSEGYTGSSISILTGTKSIDCSTL-RAQGYDSLNYPTFQLSLKTTRQPTTAVFWRQVTN 668
           LC EGY  ++++ L GT+S+ CS++    G+DSLNYPT QL+L++ +  T AVF R+VTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAKMVSGSLTWA 728
           VG P SVY ATVRAP GVEITV P +LS S+  QK++FKV+VKA  +   K+VSG L W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARYVVRSPIVIY 741
             R+ VRSPIVIY
Sbjct: 734 SPRHSVRSPIVIY 744

BLAST of Lcy13g012060 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 617.1 bits (1590), Expect = 1.9e-176
Identity = 362/769 (47.07%), Postives = 487/769 (63.33%), Query Frame = 0

Query: 3   ISKSSPLLFFLLFFVVGYVVAV---EINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSL 62
           +S     LF L F +V   V     + N  +K +IV+M +    + V A E H N+LM++
Sbjct: 2   VSNQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTV 61

Query: 63  KESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFI 122
              + +A+E  +YSY K+ N F A+L   EA+ LS+ EGV  V  N  R+L TTRSWDF+
Sbjct: 62  IGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFL 121

Query: 123 GL-PINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNN 182
           GL     +R    ES+IIVG+ DTGI   + SF D G GPPP KWKG C+   NFT CNN
Sbjct: 122 GLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNN 181

Query: 183 KLIGARYFKL--DGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGV 242
           K+IGA+YF +  +G PD  +  +  D  GHGTHTSS   G +V  ASL G+A GTARGGV
Sbjct: 182 KVIGAKYFHIQSEGLPDG-EGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGV 241

Query: 243 PSARVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFH 302
           PSAR+A YKVCW  +GC+DMD+LAAFD AI DGVD+ISISI G     + ED +AIGAFH
Sbjct: 242 PSARIAAYKVCWD-SGCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFH 301

Query: 303 AMKKAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINL 362
           AMK+ I+T  +AGN GP   +V N APW++TVAA+ +DRKF +  +LGNG   SG+ +N 
Sbjct: 302 AMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNG 361

Query: 363 FNPNQKMYPLVSGGDVA-MNPQSKDSASFCVDGTLDPSKVKRKLVFCKLIT-------WG 422
           FNP +KMYPL SG   + ++       S C  GTL   KV  K+V+C+           G
Sbjct: 362 FNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQG 421

Query: 423 ADSVVKSIGAYGTIIQSDQFLPNTDLFMAPASLVSSLV-----GETIDNYIHSTRTPTAV 482
            D VV+S+   G I+   Q L  TD  MA ++L++        G  I  YI+ST+ P AV
Sbjct: 422 QDHVVRSLKGAGVIV---QLLEPTD--MATSTLIAGSYVFFEDGTKITEYINSTKNPQAV 481

Query: 483 IYKSRQLKSAAPIVAFFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQF 542
           I+K++  K  AP ++ FS+RGP   S  ILKPDI+APG++ILA Y+ L S+TG   D + 
Sbjct: 482 IFKTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRR 541

Query: 543 SKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGA 602
           + F++MSGTSMACPH AAAAAYVKSFHP WSPAAI+SAL+TTA+P+  + N + E SYG+
Sbjct: 542 TLFSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGS 601

Query: 603 GNLSPSRARNPGLIYDLNEMSYIQFLCSEGYTGSSISILTG-------TKSIDCSTL-RA 662
           G ++P RA +PGL+YD+ E +Y++FLC EGY  +SI +LTG        K  +C  + R 
Sbjct: 602 GQINPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRG 661

Query: 663 QGYDSLNYPTFQLSLKTTRQPTTAVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLS 722
            G D LNYP+    + +T    + VF+R VTNVG   S Y A V AP G+ + VVP  +S
Sbjct: 662 LGSDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMS 721

Query: 723 LSQLLQKQNFKVIVKASPLPSAK-MVSGSLTWAGAR-YVVRSPIVIYGS 743
             +  +K+NFKV++      + K +VS S+ W  +R ++VRSPI+++ S
Sbjct: 722 FERPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFRS 761

BLAST of Lcy13g012060 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 549.3 bits (1414), Expect = 4.8e-156
Identity = 309/693 (44.59%), Postives = 442/693 (63.78%), Query Frame = 0

Query: 52  HLNVLMSLKESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQ 111
           HL++L  L    + A   +V SY +SFN FAA L++ E++ L   + V  V P++  +L 
Sbjct: 15  HLSILQKL-VGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 74

Query: 112 TTRSWDFIGLPINARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFA 171
           TTRSWDF+G    ARR++ +ESD+IVG+ D+GI P +ESF D+GFGPPP+KWKG+C    
Sbjct: 75  TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 134

Query: 172 NFTGCNNKLIGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGT 231
            F  CNNKLIGAR++            S  D  GHGTHT+S A GNAV  AS  GLA GT
Sbjct: 135 KF-ACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGT 194

Query: 232 ARGGVPSARVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVA 291
           ARGGVPSAR+A YKVC++   C+D+D+LAAFD AI DGVDVISISI+ +   N    +VA
Sbjct: 195 ARGGVPSARIAAYKVCFN--RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVA 254

Query: 292 IGAFHAMKKAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISG 351
           IG+FHAM + IIT  +AGN GP  GSV N +PW++TVAASG DR+F+    LGNGK ++G
Sbjct: 255 IGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTG 314

Query: 352 VGINLFNPNQKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADS 411
           + +N FN N   +P+V G +V+ N  S+  A +C  G +D   VK K+V C       ++
Sbjct: 315 ISVNTFNLNGTKFPIVYGQNVSRN-CSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREA 374

Query: 412 VVKSIGAYGTIIQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQL- 471
            +   GA G I+Q+     +  +   PAS +     ++I +YI S   P A I ++ ++ 
Sbjct: 375 YL--AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIV 434

Query: 472 KSAAPIVAFFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKG--DTQFSKFTL 531
              AP V  FSSRGP+     +LKPD++APG++ILA ++P+ S +      D +  ++++
Sbjct: 435 DREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSV 494

Query: 532 MSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLNPDGEFSYGAGNLSP 591
           MSGTSMACPHVA  AAYVKSFHP WSP+AI+SA++TTA+P++ + NP+ EF+YG+G ++P
Sbjct: 495 MSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINP 554

Query: 592 SRARNPGLIYDLNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRAQGYDSLNYPTFQL 651
           ++A +PGL+Y++    Y++ LC+EG+  ++++  +G +++ CS  R +  D LNYPT   
Sbjct: 555 TKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSE-RTEVKD-LNYPT-MT 614

Query: 652 SLKTTRQPTTAVFWRQVTNVGRPVSVYNAT-VRAPPGVEITVVPATLSLSQLLQKQNFKV 711
           +  ++  P    F R VTNVG P S Y A+ V   P ++I++ P  L    L +K++F V
Sbjct: 615 TFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVV 674

Query: 712 IVKASPLPSAKMVSGSLTWAGARYVVRSPIVIY 741
            +    L     VS S+ W+   + VRSPIV Y
Sbjct: 675 TISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 689

BLAST of Lcy13g012060 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 548.9 bits (1413), Expect = 6.3e-156
Identity = 323/742 (43.53%), Postives = 459/742 (61.86%), Query Frame = 0

Query: 10  LFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLK-ESQVEAKE 69
           +F LL  VV +  A + ++ K+ +IV+M   P   +   +  H ++L  +  ES ++  +
Sbjct: 13  IFALL--VVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQ--D 72

Query: 70  SMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIGLPINARRK 129
            +V +Y +SFN FAA+LTE E + L+  + V  V P++   LQTT SW+F+GL    R K
Sbjct: 73  RLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTK 132

Query: 130 TKQ--ESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNNKLIGARYF 189
                ESD I+G+ D+GI P ++SF   GFGPPP+KWKG C    NFT CNNKLIGARY+
Sbjct: 133 RNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARYY 192

Query: 190 --KLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMY 249
             KL+G P+     S  D +GHG+HT+SIA GNAV   S  GL  GT RGGVP+AR+A+Y
Sbjct: 193 TPKLEGFPE-----SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVY 252

Query: 250 KVCWSGT-GCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKAII 309
           KVC  G   C+   +LAAFD AI D VD+I++S+  +  G + ED +AIGAFHAM K I+
Sbjct: 253 KVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGIL 312

Query: 310 TVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKM 369
           TV  AGN GP   ++V+ APW+ TVAAS ++R F++   LGNGK I G  +N F+ N K 
Sbjct: 313 TVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKK 372

Query: 370 YPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGAYGTII 429
           YPLV G   A +     SA FC  G LD  +VK K+V C   T       +++GA  +I+
Sbjct: 373 YPLVYGKS-ASSRCDASSAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASIV 432

Query: 430 QSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRTPTAVIYKSRQL-KSAAPIVAFFSS 489
           + + +     +F  P S++S      + +Y++ST+ P A + KS  +    AP+VA +SS
Sbjct: 433 R-NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSS 492

Query: 490 RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 549
           RGPNP  + ILKPDI APG +ILA Y+P   +   + DT+  K+T++SGTSM+CPHVA  
Sbjct: 493 RGPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGV 552

Query: 550 AAYVKSFHPLWSPAAIRSALLTTASPISRRLNPD---GEFSYGAGNLSPSRARNPGLIYD 609
           AAY+K+FHPLWSP+ I+SA++TTA P++   +P     EF+YGAG++ P  A +PGL+Y+
Sbjct: 553 AAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYE 612

Query: 610 LNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRAQGYD-SLNYPTFQLSLKTTRQPTT 669
            N+  +I FLC   YTG  + +++G  S  C+  + +    +LNYP+    +  T+ P  
Sbjct: 613 ANKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGTK-PFK 672

Query: 670 AVFWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSLSQLLQKQNFKVIVKASPLPSAK 729
             F R VTNVGRP + Y A V     +++ VVPA LSL  L +K++F V V  +   +  
Sbjct: 673 VTFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAEN 732

Query: 730 MVSGSLTWAGARYVVRSPIVIY 741
           +VS  L W+   + VRSPIV+Y
Sbjct: 733 LVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lcy13g012060 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 543.1 bits (1398), Expect = 3.4e-154
Identity = 326/752 (43.35%), Postives = 457/752 (60.77%), Query Frame = 0

Query: 2   SISKSSPLLFFLLFFVVGYVVAVEINEGKKHFIVFMEDQPVLNEVNAVETHLNVLMSLK- 61
           +++ SS LL  LL   +  V AV   + K+ +IV+M       +      H+N+L  +  
Sbjct: 3   TLAASSSLLSCLLVLFLSSVSAV--TDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 62

Query: 62  ESQVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREGVSHVIPNRYRKLQTTRSWDFIG 121
           ES +E +  +V SY +SFN FAA+LTE E + ++K  GV  V PN+  +LQTT SWDF+G
Sbjct: 63  ESSIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 122

Query: 122 LP--INARRKTKQESDIIVGLFDTGITPTAESFKDDGFGPPPRKWKGTCLHFANFTGCNN 181
           L   I  +R    ESD I+G+ D+GITP ++SF D GFGPPP+KWKG C    NFT CNN
Sbjct: 123 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNN 182

Query: 182 KLIGARYFKLDGSPDPLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPS 241
           KLIGAR +  +G+          D+ GHGTHT+S A GNAV  AS  G+  GT RGGVP+
Sbjct: 183 KLIGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 242

Query: 242 ARVAMYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAM 301
           +RVA YKVC + TGCS   LL+AFD AI DGVD+I+ISI       +  D +AIGAFHAM
Sbjct: 243 SRVAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAM 302

Query: 302 KKAIITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFN 361
            K ++TV +AGN+GP   SV   APWILTVAAS  +R FV+   LGNGK + G  +N + 
Sbjct: 303 AKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYE 362

Query: 362 PNQKMYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKRKLVFCKLITWGADSVVKSIGA 421
              K YPLV G   A +    +SA  C    +D S+VK K++ C     G   +V+S+GA
Sbjct: 363 MKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGA 422

Query: 422 YGTIIQSDQFLPNTDL-FMAPASLVSSLVG--ETIDNYIHSTRTPTAVIYKSRQL-KSAA 481
            G I ++    P  D+ F+ P      L    E++ +Y+ ST +P A++ K+  +    +
Sbjct: 423 VGLIYRT----PKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTS 482

Query: 482 PIVAFFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSM 541
           P++A FSSRGPN  +  ILKPDI APGV+ILA Y+P    +  + DT+  K++++SGTSM
Sbjct: 483 PVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSM 542

Query: 542 ACPHVAAAAAYVKSFHPLWSPAAIRSALLTTASPISRRLN--PDGEFSYGAGNLSPSRAR 601
           +CPHVA  AAYVK+F+P WSP+ I+SA++TTA P++         EF+YG+G++ P  A 
Sbjct: 543 SCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAAS 602

Query: 602 NPGLIYDLNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRAQGYDSLNYPTFQLSLKT 661
           NPGL+Y+L++  +I FLC   YT   + +++G +++ CS  +     +LNYP+    L  
Sbjct: 603 NPGLVYELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILPRNLNYPSMSAKLSG 662

Query: 662 TRQPTTAVFWRQVTNVGRPVSVYNATVRAPPG--VEITVVPATLSLSQLLQKQNFKVIVK 721
           +    T  F R +TNVG P S Y + V A  G  +++ + P+ LS   + +KQ+F V V 
Sbjct: 663 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 722

Query: 722 ASPLPSAKMVSGSLTWAGARYVVRSPIVIYGS 743
            S L S    S +L W+   + VRSPIV+Y S
Sbjct: 723 GSNLDSEVPSSANLIWSDGTHNVRSPIVVYTS 730