Lcy13g011650 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g011650
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionSerine/threonine-protein kinase RIO1
LocationChr13: 13837759 .. 13875120 (-)
RNA-Seq ExpressionLcy13g011650
SyntenyLcy13g011650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCAAGGAGTTTACTATTTTCCCTGGTATTTCATTTGATTCTTCGTTTCTCCGTCCCCTATTTCAACTTCTATAACCTTCCTTTTCTGTTATTTTTATTTTTGCTCTTTCTGCAATGTTCTTATTAGCCCTAATATTTGTAAAAGGTTTGTTAATAGAATACATGATATATACCAACACGAGCATCAGTGGTTAACCTTAATATGTAACTCTTCTTAGGCAGTTAGGCCTTGTAATTCTTTGAGTCGAGTCCCTACCTTTTATTTTCTTTTTTTCTTTTGTGCGCAAACTTACCTGTATACAGATTCCTGAATGATCTTGTCAATGATACAATATCAACTCTAGTGGGAGCAGCAGGTATGTCTGCACTGGAAGAGAAGCTAGATGAAATCGAACCTTCAACAAGGAATAATGATGAGTTGGAGGAAGAAGATGAAGTAGAGGAGGACATTTCATGGTCATCAGACCCAGAGATTGGAGATGCATTGGATTGGTTGGATGCAAAAGAAGATATCTGATAGCACCAAATTGGTGCTAATTATTTCCGTAAATTTTGCATATCCTAATGCTGCTATTTGTGATATTTGACTAATTTTGAGTCTTATTGATGCATAGGTCAATTTGGTGTTCTCGGGATCAAGGAAGGACATTTTGGTAAATAAAAGTAAGGCAAATTTGGCCAAAGAAGCGAAAGTCAACCAAAAAGTCAACAGGAACACAAATGAACTAGAGGTGCGCCCAAACACCGTCAGCGTCGAGACGCTGTCACGAGAATATAAAAGTCACATTTCGCATTTTAGGTCATTCATGCTCGTTTTTCTTCCGATTTTGACCGCATCACCTCATCTCTCTCTGTATTTTTCCAGTTCCATTGTATTTGCATTAGATTAGATTAGTTTTTAGATATAGGAATGGTTAATCGAGCCCCGGCGTTTAGTTGGAGCATTGCAATGTCCGGCTAAGTGTAGATCGTCGTAATTGGGGACTCCGAATGTAAGCTTGGCATTCCTTTGAGACCTTGTATATTTCATATTTCATAGAAAACTATTGTCTAATTGTGTTCTTAATTCCAGTTTGTATCACAATTGGCTTGAATGTATAGATTGACAACCTAATTTGTTTGAACCAATTGCTGTGCTAGAATTTGTAGCAAATAAATCTATTGTTAATCTTGATTAAAAGGCGTTTTATCCCAATTGATCAAAATTGCATTATAGATTTAGGAGTTAAGGCTTAGAATCGTATGAATTTAGGCTTTTCCTAGTTTATGCATTCTATCTAACGTTCCATCATTCTTAATGCGATTAAATTCTAATTAGTGAGACGTCATTGGTTAGATTTTTAATTAGGTTCTAACCTCGATGCTTTTGTCGGGTTAGAAAATAATTGGGATCATTAATCTCACTGGCTTTTCCAGTGGGAGGTTAGACAATTAGCTAGTAATTTGTTAATAAAGAGAGTCTATGATTTGGAATTGCCAAACTGAATTCGGATAACCAATTGCACGATCCTGAGGTAAGATTAGTCGGTTCATCCTTTTATTTTCTAGTTATTTAAAATTTAGTAATTTAATTTCTAGCTATTTTTATCTTTCATCCTCAAATTCTCTTCCATGCCAAACCACCCCCCTCCGGTTACTAATTGAAAAATCTTAAGTTAATTTAATTAATTTGATTGCTCCCTGCGTTCGACCCCGACCACTATTTCTACAAAAATAGTTGTAGTGTCGTGGAATTATAAATTTATTTGACCCGAAGCCTCGACAACTCTGGCCCGTCAATATCGAAGTAGTAAATGGGGCTTTTTCACTCAATGTTAGGCATCCTAATGCTCATGGTGGGCTTTATTCTCGTCATAATTCCTCGACTCTTCAACCTCTCTCAAATCGGAAACAAAAGTTTACCAATCACATTCGAGTTTCACCTCTTACGGTAATATACTTAATTTTCTAATTTTATATTGTTGGTTGTGTTTTCTTTTCTACTGACAAGATAAGGCAACAAGTTGAACACTCATATATTGTTGGCATGAGCGTCAGATGCCTAAGCCCTTTATGAACCATCATTCTAGTATCTTCGTATAGTTTTATACTATACTTGTAATTTGTAAAGGAATAGATACTACTATTCTTAATGCTCATATTCATAGATTACCTGTAACTGAGGGACACCTTAATTATTTATCATGTTTGTTATTGTAGGAATGAGAAGAGAGGTTTAATGTCGGAATGTCAAACTCTGTTACCACGACCATTCGTGACAGTGTTAAGGAAATGGCTATAGGAAAAACGAAAACTACAGAGAAAGCTGATTGTGTAACTTATATTTTTAGTTATCAACTGTGTTATTTTAGGTTCACTTTATGATAGAATTGAAGGCTGATTCTTTGCGATCTTGATCGAGGATCGGTTTGATTCGAGTTTTTGGATTACTGCTGAAGGAATTGAATCCCAGCAGAAGCGGTTTTCACCGAGCGTGAATGAATTTAAGTAATTAGAAAACCAATACATCGGGAAAATTTACAGAAACGATTTCTATACAACACAGATTAAGCATGCTAATTACTAGATCTAAACAATTTAGATTTCCGAAGACTTACCTGATTTAACAGAAGAACTGCATACGCTCCAAAATCACGTACTGTGAATTTCTCCTCCAAGAACACTACCAATCGCGAACACCTGCTATATCTCTAGGGAGAGATCTTGATGGTGGGATCAAAAGAAACGTCTTGGATAGGGAATTTTGCAGCAATCAATCGCTGTCGTTCTAGGGAGAGAATTTGAGAGAGATCTTCTGTGATAGCAGTAGCATTATGCGTGAGAGTGTGTGAGAGAGAGTAACCATCAGAACAGTATAATACGTGGAGAGGGAAGTTAAGTTCTCTAATAGATCTTGGCCATTGATTTTTTTTTTTTAGGAGTACAAAACAGAAAGACTAAACATGAAAAGTAAAAATTACATCCGAAAGAGTCATGTCATGAACTAAACTGCTGAGCCCAAGGGGGACAATCCTTGATCCAGACTTCAGAGTGCTTCGATGTGCGAGCGTGAGTGGCTAGACTATGGGCAACTTTATTCCCATCTCTAGGAGTAAATAAGAACCTCTAGATTTGGTGGCCATGGAATAACTGCTTGAGATGATCAATGAGTGGAGCGATCTCATTGAGATGTTGGTCATGGTCATGCAGTAGATTCCAAACTATTTTAGAATCTGTCTCCGCCCAGATAGGTGTAAGTCCATATTCCAAAGCTATTGTTACCCATCTATAAGAGCTTGAGCTTCCACCCATTCCACCGAACAATATGTTCTCGTGAACACTTCTCTGTAGATGCCATTGTCTCTCCTTTGGAATTTCGAATGATAATGCCTGAACCCATCTCGCCATTAGAAAATGCTACATCCACATTCAGCTTGAAATGGGTCTGTGGTGGTGGGGACCAGGTTGCATAATGACCAGAATCACCAATCGTCGACCTCGTCTCTCCATTACTTCTATTTGGTTGAGCATCTACATATTCAGAGATGTAATTTGATGCCCAGGCAATAGAATCATCTGTCTGTCTATTTGGGAGTGAGTTGCCCTGAAAAACTGCATGGTTTCGATTGTTCCAGATGGACCACATTGCTGTTGCAAAAATCAACATATCTTCCATACATAGAGTCTCTTGGGCCCTGCAAAAGAGATCGAGGATATTAGTAAAACGATCATTAAGGAAAGTTTGTACCAGACTCCAGCAAGCCCAATGCTTTTTCGTCTTCTTACACCCCCATAGAGCATGGGTTACTGTCTCATTTACTTTTCTGCACATGGGGCAACATGGGTCACAAGAGCTATGTCTTTTTATGAGATTCATCCTGCATGGCAATATGTCTAAATTGGCCTTCCAAACAAAGATCTTAATTTTGTTTGGTATGTTTAGATTCCAAAGTAGATTCCACCAACTGGAAATAGATGAATCAGACGATGAAGATTCTCCCTGGAAATCCTCCAAAGCAAGCTTGTACCCACTCCGGACAGTGTATCGGCCCCGCATTGTCATAATGCCAGAAGAAGCTGTCTAGGTGTTGAGCACCTGCAATGGGAATTTCTTGAATTTTTTCTACAACATCCTGCACAAAAAGATCTGTCAGTAAATTCACATTCCAGGATTGACTACTTGAGAAAAGACTTTTGACTCGATAGTCAGTCAGAGGCCTTTTGAGAGAGAGAAGTTTAAAAGTCTTCATCCTTGGAATCCGTTTATCCTCGTAGATGTCAATGGTTTCGCCATTCCCAATCCTTCAACGACCACCTTTCATTGCTAGTTCTCGACCCCATAAAATTCCTCGCCAAACATACGATGGTCTGCCCCCCAAATTTTCTTTCAAGAAATCATGATGAGGATAGTATCTTGCTTTCAGAACTTGTGCCACTAGAGATTGGGGTCTTTGTAGAAGTCTCCAAGTGTGCTTCGCCAATAAAGCTTGATTAAAAAGCCTTAGATCTCGGAAACCCATTCCGCCTCTTTCCTTTGGTTTTGTCATGATTCCCCATTTCAACCAATGAATTTTTCTCCCTTGATCAGAGCTTCCCCACCAGAAGTTAGCCATCATCCTGTTCAGGTCGTCACAACAACCTTGTGGAAGTTTAAAGCATGAGAGGGTGTACGTGGGAATTGCCTGTGCGACCGCCTTTATAAGAGTTTCCTTTCTAGAGGATGAGAACATCTTCCCTTTCCAACCCTGCAAAACTTTTTGGACCTTATTTTTGATGTAATTCAACGGAGCTTTCTTGTTTCTCGAAAGGGAAGATGGGCAGCCGAGATACTTTCCATGGTTAGCTACTATTTTGACCTTCAAAATATTGGCTAACTCGCTCCGTTTGTTGTTTGCAGTTCCAGGTCCAAAGGCTATTGCTGATTTTTGAAAGTTTATGATATGACCTGTGGCCTCCTCATAGACTTTTAGAAGCCTTTGTAACTCCACAAATTCCTCTTTGCGCGTTTTCAGAAACAGTAAGCTGTCATTTGCAAAAATAGGTGAGAAATGGCTGGTGCCGTTCTTGCAATTCTAAGACCTGAAATATTTTGAGAGATCTCAAGCTTTGTTAAGAGGCTAGATAAGCCTTCAACACACATGAGAAATAAATAAGGTGATAGAGGGTCGCCCTGCCTAAGTCCTCGAGTGGGCCTAAAAGTTTCCGAACATGTGCCATTGATAAGGATAGAGAAAGAAACAGATTCAACACACATTCTAATCTTTTTAATCCAAGATGTATTAAAACCCATTTTTTCCATAATGCGATATAGAAACGACCACTCCACTCTATCATATGCTTTGCTCATGTCTAATTTGATAGAAGCATAGCTATTTTTCTTGGATTTTGAACCTTTAATGAAATGTAAGCATTCATACCCAACAATAATATTATCAGCTATAAGTCTATCGGGGACAAAAGCACTTTGGTTATCAGATATAATAGAGTTAAGGATCAATTTCAATATATTAGCAAGCACTTTAGCAATAATTTTATAACAAACATTGCATAAACTGATAGGGTGGTAATCGTTCACCGTGCTTGGGTTTTTCATCTTTGGAATGAGGACTATCGTTGTGGCATTGATCTGTTCTGGGATTTCTCCCTCGTTAATGAAGGGAATAAAAGTCCCCACGCAGCGGAAGCGCATCGATTGGACCTTACGCCGCATATTAATTAACATTAAAATACCGTTACGAAAACTCAAACAAACTTTAAACACTCTTTAAAGTTGCTGGAAAATCAGTTTAAACATGCTTAAAAATAAATCTAAATTTATTTTCATGCTTTTCTAAAGAAAGAAGGAAGAACCGTTACAAACCTTCGTTGTAGACTTGACCGTTCTTCGCCAACGACGCTCCACGAACGAAAACGCAGCAGCCTCCTTTTCTTGTGATCGCTGGAAATTTCTCCTTTGAATGACACAATAACCACAGAGGTTACACCGGTATGTCTCTAAGTCTAGGAGGCAAAAGACTTGTGGGAGTCTTTCTCTAAGAGGGAGGCTAGAGAGAATTTTAGGTCTTGTCTTTGAGAGTCTAAGAGCAAGAGCAAGAGTGTCAAGTGTCATGACCAATTAGGTCTAGTGTTCTATTTATAGAAGAGAGGCCATGAATAAAATATTCAATTAAATCAATCTTTTCCAAAATTGATTCAATCTTATTCCTTAATTAATCAATCATGATCAATCAATTCCATAATTGATTAATCTTTTCCACAAATGACTAATTACTTCCATAAGTGATTAGATCATTTCCATAAATGATTAATCACTTCCTTAAGTGATTAATTATTTCCATAATTGATTAATTCCCGGTTAATGAATTCTTTAATTAATTAACCACCAATTAAATATCACATATTTAATTTCCGGCCTAGAATTAATTTGCCCAAACACATTTTAGGCCTTAAATTAATTCATTATTTTGAACTCTTTCAAACTTCCATAAATTTATATTTGAACTTCTTCAAATATTAAATTCTCTCATAGTAATATATCAATATATATTATATTTGTTTCCCTTAGTAAATTGAACATTTTCAATTTATTATTTAGTTGCCTCAAATTACTTTACGAGCTACCAGTGGGACCTAATGGACCTGCAGATCAGAAGCTCCAACGATGTGAGATTAATTGGTCAAACTCATTAACCACATTAATCAATATTCGTTAACTGTGTGTTCATTCCACTATAGACTCACAGTTGCACTCTTCTCACTGCAGACATATTTTCTGTGTCCACGGATATAGACCAATATCGATTAGTTAGTCCTTCACGAGTGTCCGTAACACCAGCTGGGTCAAATTACCGTTTTACCCTGGGTTACCTCTATTTCCTTAAGTACCAATTCTCCTCTAATGAACAACCTGTTTGTGGTCCAACCATCAAACAGAAAACCCTCTCGTGCCATTGAGAGGGTAGGGCCCTTTGTTCAAGACTCGGAGTAACCACTTAAGGGAACACTCATCTGCTTCCCTAAAGTCGGGAATGAGTGAATTCCATCTTGTACGATTATGTTCCCAGCTCCCCATTCGGTCTTGTCCCCAAAGTGGTAGGCTTATTGAGTCGGCGTATTGGCCACTCTCACCCATACAAATCAAAGGACAATCTCTCATGAGCAGGAGTCCATAATCAACTCAGGATTAAGACTAAGTTGTCTAGGTCGTCCTAGTGAAATAGAAAACCAACTAGTTAACGGTGTTATAACCAGTGGTTACTATTTCGCGGTCCGGTCTTATGCAAACTCATTGCATAGGATACCCCCACTTGCATGTCTCCTACACGAACGCGTTGGATCGCAGCGTTTGTATCAAATACAAAGTGGGTCGTATCCATAGTGTCACCAGGATAAGGCACCCAGTCTTATCCCTATACTATAGACCCTTTAGGCTGTTTCTTGAACAATGATCCCATATGTCGCTACATATAGTTCAAGACTCATATTACAGCCTTGGATGTCTAGTTTATTGGATTAGGGTTATTATTAGGCAAAGACAAATATAAAATTAACTAATAACAATTAATGGAAAAATATATCAATATTATTTCATTAATATCCGGTCAACAGTTACATTCACTATCTACGAGTTTTAGGGCATAAAACCCAACAGTTAAGACATTTGAGGCATGTACTGGTAACTTGATCTCCAACAACATTCCAGTATCTTTGATAAAATAGAGCTGGTAAACCATCTGGTCTAGGAGCTTTTGTTGGATGCATCTGCTTGAGGGCCTCCTCCACTTCTGACGTTGTAAAGACCCGATCTAAACATGCATTCATTGCGTCTGTTACCCTAGCTGTGGTAGCTTGGATTGTTTCGTCGATTGCCTGCATATTTAAGGGAGAAGCTTTAAACAGTTCAGAATAATAATGAAGGAAAAGTCATTCGATATCTTCCCTATTTTCATAGTTGTTTCCATTGAAGTCAATTATCTTTTGAATTTTATTTCTTTGACGACGTAAACTGGCCTTAGAGTGGAACCATTGATTGGTATCAACAAGATTTGGACCGTTGATTATTTTCCCATAATAATTAATAATCCAAATAAGTATAAAATAATTTAATTAAACTAACTTTAATTAAATTAATTATAACATAAAGTTTTAATTCAATTAAATCATATTCAATTGAATTAATTAGGTCAACCAATTAATTCTCAATTAATTGTTCCCCACATTAATTAAATATCATATACTTAATTAATTTTACATTTGAATCATATTCAAATGTACCTCTCTCATTTAACATATAGTTTTAATGTGCATCATATGTACATTAATTATTCACTACAGAATTAATTTGATTAAATCACATTTAACCAAATTAAATTAAATTAATTCTCCAATTAATTAATTCATGAATTAATCAAATATCATGTTGCCACGTGCCTGCGATGCAGAGCAACCGACGCCCTCGAAAAGGGGTAAAATAAAAGGAGTCGCCACCAATCTTTTTTATGGTGTGATTAGACACCAAAATAAAATAAAACTATCATTTTAAGAAAAAAAAATAATAAAAAATGGTATGCAAAAACCATATTTGGGTTCGGGAGTTGTTTGTGTATGGGGGAGGTGTTAGCACCCCACAACACCCGTTAAAAACGGTGGCCAAAATTTTATCTTGAATTGAAAATAATTTAGAAGAAAATATTATTTAGGCAAAAATATATTTTTTAAAATAATGTCTTATTTTTACCCCTCATTACTCCAATATTTGAAGTAATGATCCCATAAAATAAGTGGAAAATTATCCATTGATTTTGTTATATTAGAATTAAAATTCTTAACCTCAAGAATATGAGAGAATTTTACCATTCCCTAATTTCGTCATAGGATAGCATTCCAAAAGGAAAGATTTTTTTAAAAACATTTTTTTTAATTAAATTAATTTTTTCTTGGGATTAAAATCTCGGACTCCCAAAGATTAAGATCCCTCCTTAAGAATTCTAGAAAATCGGACTCCCAAATATTTCTAGATTCCATCATAGGATTTTTTTTAGGAAAAAAACGGCCTTGAAAAATTATTTTTAAGAAAAGATTATAAAAGCCTTTGAAAATAACATTTTAATGATAAAACCTTTATAAGCTCAAAAAGAGCAAAGAAATTAGAAAATAAGTTTAGAAATAAATTTAAAATAAATAAAAGGAAAGTTTGAAAAAAGAGAATTTGAAAACAGTTAACAAAAAAAATACATATAATATACAACATTTATATGCAAGCACAATAGACAACAAATTGCAAAAAATTTTTTTAGAAGAAATTGCAATTATGATACAAGTAAATCAATACAATAATTTATGCAATAAATAAACAACCAATAACATATTCAATTAAGTTAAAATTTAAGAAAACATATTGGAATCGATCTCGGACTCCCAAAGAACCGACTCCTCACCTCAAGAAAAACTAGAAAATCGGACTCCCAAATATTTCTAAATTCCGTCGTCGGATATTTTTAATGAAAAGAGATATGGATTAAAGATTAAAATCACATTAACAAACACAAATATATATCAAATTAACAACCATGGTGAATAAGAGAACAATTTATATTTAACCACATAATTATTAACAAATGATATAAAAAGATAATAATTAAATACAATGCTCAATTAATATATTCTAAAAAATTGCTCAACTAATATAGTTAAAACATATCAATACAGAAATTAAATTCTAAAAAGAGTAATTTAAAAAAAGTATTAAACCAAAAGAGAGTGCATTAATGAAACATAGAAAGATATAATATATAATGAAAAAGAAAAAAAAAATGAATTAAAGAAAAATTATTTTTTTTTTAAAAAAGAAGGAATGGGCGACAAAATTGCCGCCCATTCTGTGCGACGTGAGCCCGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACTCAGCAAACTCACGCACACGCATATATAATAATAATAATAAAAAAAGAAAACAAAATTCCACCTCTCTTTTCTTTTCTCAACCTCACTACCTTTAGCTTTCTTTTTCTCTTCTTCTTTTTTGCTCAGATTTTTCTCTCTTCTTCAGCTCACCTCCCTCAACAACCATTGCAATTTTCTCTTGCAAACATAGAAGCTCTCTTCTCTCCAACTAAGGTATGCACACTCGGTTTTTTCTTTTTTCTTTTTTCTTTTTTTTTTACATACATTTTTTCACATCTATCTTCCTCTTACTTCTTTTTTTATCTTCCTTTTTTTGTCATATTCTTTCTAAGCATATCTCACTCTTGTTTTTTTTTTTTTTACTAGATAGAACTAGAAAGTAAAACTTTAGAGAGGTAAAAAACGAAAAATAAAACATAAGCAAAAGATAAATAAACAAGAGCTCACAAAAATATAAATAAGCAAGAGTTCATATAATGAAATAACGAGTAAGAGAGAGTGCAAGTGAGAAAAAAAATAACAAAAATAACAAAAGCAAATTTCTAATGTGCAAAAACTCACATAATAACGAGCAAACGAAAATGTGGGGAGAAGAAGAAAAGAAAATAGATGCAACTTCTAATACCTTACTCTAACTCGTTTTTCCTTTTTTGTGCTTGAAGAAGGAAGAAGAAGCAAAAACAAAAGGGGAAGAACACTAAAACAACTTGTTGAACAAGGTGTGGATCCTTGGGACAAAGTCCCTACCAATGCAACCTCCAACGTAGTTTTAGTTTTTTTGTGTGTTCTGATTTTTGTGGTTTGGTAGAAGGATTTTTTTGCTATTTTTTTTGTGGTGAGGCTCTGTGTATTGTATGTGTGCTGGTGGATGTGTATAGTGTGCTTTCTCTCTAGTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGTTGAATGAATTCTCCTCTCATTTATAAAAGCTCAAACTATTTCCATTTTTTTGCATGAAAGTTACTAGCTATATTTTGAATTTAAAGACCATGCCAAAATGGGTTTACACCAAAGAGGACATAAGCTTTAGCCAACAAAATTCAAAATTTCAAAATTTTACTCATTTTAACCCTTGCAACCAACTAAATTGGTTCCTCTAACCAAACTAAGAAGAAGACCAAATGGCATTAACAAACATTTCTAATTAAATCAAATTTGCAAACATAATTATGAATTTAATGATTAATTTAATAAGCCGGACAAAAATAGGTGGCTACATATCACATATTTAATTAAATTCAAACTTGAATCATATTCAAATGCATTCTCTCATATACTATAGTTTTAATATGAACTCTTATTCATATTAATTTTAACCTATAGTTTAATATGAATCCAATTCATATTTAATATTTTAACTCCTTCAAATATATAATTCTCTCATTAATTAATTTTGAATCACATCCAAAATTAATTTTATTATAAAGTTTAATTCACTAATTAAACTTATATTATAATGTATCTCCATACATTATATTAATTGTATCAAATACAATTAACTAAAATTTCCTTAATATTCAAGACTAATTGATCTTAGAACCCTTTACGAGCTAGCAGGTGGACCTAATGGACCTACAGATCAGAAGCTCCAACGATATGAGATTAATTGGCTAAACTCATTAACCAAATTAATCAACATTCGTTAATTGTGGGTCACTCCACTAAAGACCCACAAACTGCACTCTTCTCATTGTAGAATATTTTTGTGTCCACGGATATCGACCATACTGGTAAGTCAATACTTCACGAGTGTTCGTAACTCCAACTAGGTCAAATTATCGTTTTACCCCTAGGTTACCTCTGGATCCTTAAGTTTCACTGATCCCTCTAATGAACAAGTTGTTTAGGGTTCAACCACTAAACAGAAATTCCTCTCGGGCCAGGAGAGGACAACACACTATTGTTCAAGACCCGGAATCATCCCTTAAGGGAAGACACATCTACTTACCCCAATAGGGGAAGGAATAAATTCCATCTTGTACTGTTATGTTCCCAGCCCCCACTCGGTCTTGCCCCTGAAATGGTAGGATTATTGAGTCGGCGAACTGGCCACTCTCCCCCATACAAATCTAAGGACAATCCCTCCTGAAAAGGGGTTCATAACAGACTCAGGATTCAGATTGTGTTACCCAGGTCATCCTATTGAAATAGAAACCTAAACTAGTCAACGACGTTACATCTAGTGGTTACTATTTCGTGGTTCGGTCTTCTGCAAACTCATTGCATAGGATACCCCCACTAGCATGTCTCCTACATGGATGTTTTGGATCAATGCATCTGTATTGAATACAAAATGGGTCGTATCACATAGTGTCACCAGGATAAGGTACCCGACCTTATCCCTATACTATAGACCATTTAGGTTGTTACTTGAACACTGATCCCCGTATGTCCCCACATACTAGTCAAAGTTTCATTTAAACAACCTTGGATCTTCGTTTATTGGATTTTAGGGTTAATTAATGCAAAACTCGATTATTCAATAACAAATTACTAAATCTCTCAAATAACACTTTATTGAGTTTAATTAATTTGTTTCATTTACTATCTACGAGTTTTAGGGCATAAAACCCAACAACTGCAATATAATTTTTAACCGATTCTGCCTCTCTTCCAGTCATATATTCCAAAGACACTGGATAGTGTCAAACATGCTGAGGAGGATGTAATACGTTTAACAAGTGGTCAGGACACTAAAGATATGTACTACAAGACCATTACAGGGTTAAACAAAGCCCTTCCCAGAGTTGAACCTGTGTCACAGCTAAAAGATGAGGAAGAACACAATGCAGACCCAATGGAACAATCCAAAAATTTAACCGGTTTGCCAAATTCTTATGAGAAAGTGACTGAATCAACATCAGAGGAAGATGAAGAGAGCTCGAATGAGTCAAAAGGCGGACCAGGTTCTTCAACCGAGACGACGTAGGCGGCAATTGACTGGAAAACTACCAAAAAAGAGAACAAAAAGAAGGTGAAGGAAGAGAAGAGAGAGGCCCACAAAAATAAGGTTCCAAAGGCTGTAAAGAAACGAAAGAAGAAGTTGGCCAAGTCCCATAAGTAAGGTGAAAATTGGTTCAGTTAGGTCAATTTTGAAAGTTGACCGTCATTTGAACCAGATCAGTTAGGTCAGTTTTAAAAGTCGACCGTCGTTCGAATCGGATCATATAGAATTCAAGCATCGTATCATGTAGAATTCGAGTATAATGATGCCTGCAACTTCTTTGGTTTGGTTCAATTTTGATTTATGTTCTATGATGTAAAGTTTAGTTTTGATGTAGTTTATTGTTCATTTATCTTAGTTTTCATTCGACGACGGTATAAATGAAAATGGAGATAATAAGATTTTGCTAAGTGATTTATGAGAATCTATTATGAAGACACAACACTCTCAAATTTGTAATTTGTTGAATCGATTAAAACATAATATCCTCAATTAATAAGTTGAGATTCAATTCCTCACCCTTATTAAGCTAAAACAAAGGTCACGTAAACTTTTATATTTCAATTTTCCATTTTTATTACACATTAAAACATGGTTTGAGTTATAAACAGAGTTGTACTTCTATAAAACTAGTTAATACTCATATGATGCACATTTTTTTAGTTCAGCCATACATGTGTTTTTAGAGTTTAAAAATAATGATGATTTTTAGGTTGTGTTATTAATTAATTTGTCAAAAAAATATGTTATTGTTGATGTAGACCATGTAACAGTTTAGTGTTTTTATATTATCGCGTCATTTTCATCATAATCTACATGTAGGATTGTTTATGTAGTTGTGTGTTTTATATAGTGCTAATAGTCTCTTTGTATTTTATATTTCTAACATACATGTAATAAACTAGTTAGCTATCATTATTTTAAATCAATAATTAATGTCAACTTGAGCTCCAAGTCAACTAATTAAGGTATAAGTCCTTAACCAAAAGGTTAATGGTTCAAATCTTCACCCTTACCTGTGGCTAGAAAATGAGTTGTTTACAATTTTTAACAAAAATTAAGTCATGCATACATTGAACTAGATTAGTGCATAACAAGGACCATAAAATATATCTTCAAAACCTTACAAAAAAAAAATCATGCAAACATGAAATTGTATCGACAAACGCTACTTTCTTGGAGGAAGACCACAGGAGGGATCATAAACCACGGAGTAAGTTAGTATTAGGAGAAGCTACAGACGAGTCAACAAGAGTTGTTGATGAAGCTGAACCTTCAACAAGAGTTGATGGAGGGGCTAGTTCTTCAAGTCCGTCTCTTCCTTCTTAAACATTGAGAGAGCCTCGACACAGTGGGAGGGTTGTATCACAACCCGACCGCTACTTGGGTTTAACTGAAACTCCGGTCGTCATACCTGATGATAGCATTGAGGATTCATTGTCTTATCGACAGACAATGAATGATGTAGACAAGGACCAATAGGTCAAAGTCATGGACCTTGAAATGGAGTAAATGTACTCAAACTCAGTATGGGAACTTGTAGATCTACCTGAAGGGGTAAAGCCCATAGGGTGCAAATTGATCTATAAGAGAAAGAGAGATGCAGCTGGGAAGGTACAGACCTTCAAAGCTTGACTCGTAGCAAAGGGTTGTACCCAGAGAGAAGGGGTTGACTATGAGGAAATTTTTTCTCCTGTTGCTATGTTAAAGTCTATAAGAATTCTCTTGTCCATAGCCACATATTATGACTATGAGATATGGCAAATGGACGTCAAGACTGCCTTTTTGAATGGTAATCTTGAAGAGAATATCTTCATGTCTCAGCCCGAGGGGTTCATAGCCCGAGGTCAGGAGCAAAAAGTTTGTAAACTGAATCGATCCATTTATGGGTTGAAACAGACGTCTAGATATTGGAATATTAGATTTGATATTGCGATCAAATCATTTGGTTTTGACCAAAATGTTGATGAACCTTGTGTATACAAGAAAATCAACAAGAACAAAGTAGATTTTCTAGTATTTTATGTGGACGATATCCTACTCATTGGGAATGATGTAGAATACTATACTGACGTTAAGAACTGGCTAGCGACCCAATTCCAAATGAAAGATTTGGAAGAGCACAATATATTCTTGAGATCCAGATCATTCAGGATCATAAGAACAAAACGCTAGCATTGTCTCAAGCAACGTATGTCGACAAGATGTTGTCTCGATATTCGATGCAAAACTCCAATAAAGGGTTGTTACCTTTCAGGCGCGGGGTTCACCGATCTAAGGAACAATGTCCTAAGACACCTCAAGAAATTGAGAATATGAGACGCATTCCCTACGCCTCAGTAATAGGTAGCTTATTGTATGTTATGCTCTGCACTAGGCCTGACATATGTTATGCAGTGGGAATAGTCAGTAGGTACCAGTCCAATCCAGGGTTAGACCACTGGGCAGCGGTTAAGAATATCCTCAAGTATCTTCGGAGAACGAGGAACTACATGCTGGTGTATGAGGCTAAGGATTTGATCCTTATAGGATACACCGACTTTGACTTTCAATCTGATAAGGATTCTAGGAAATCCACATCAGGGTCAGTATTCACTCTTAACAAGGGAGTTGTAGTTTGGCGAAGCATCAAACAAGGATGCATAGTAGTCTCAACCATGGACGCTGAGTATGTTGCTACTTGTGAAGCAGCAAAGAAAGCAGTTTGGCTCAGAAAGTTCTTGACAGATTTGGAAGTTGTTCCAAATATGGACTTGCCCATAACATTATACTGTGACAACAGTGGGGCTGTATCCAATTCTAGAGAACCTCGCAGCCACAAACGAGGAAAACATATTGAGAGGTAGTATCACCTGATCCGTTTACGAAGGCTCTCTCGGCTAAAGTGTTTGAGGGTCATCTAGAAGGTATAGGTCTACGAAATATGTATGTAGTATAATCTAGGACAAGTGAGAGATGTGTAATGGGTATAAGGATGTCCTAGTTTATTGCATTACTTGTATATTTACTTTTCATGTAATATACAATGTACATTACTTCCCATTGACTAAGAGTTTTAGTTCAAGTGGGAGTTTGTTGGGTTTTATGCCCTAAAACTCGTAGATAGTAAACGTAACAATTGACCGAACATAAATAAAGTATTGTTTGATGTTTTTTCAATAAATAGTTATTGTCTATATTATGTATATCTTGCCTAAATAATCGTAATCCAATAAACTAAACATCCAAGGCTGAGGGATGAGTCTTGAACTGTATGTGGATACATACGGGGATCAATGTTCAAGAAACAGCCTGAAGGGTCTATAGTATAGGGATAAGGTTGGGTACCTTATCCTGGCGACACTATGGATACGATCCACTTTGTATTTGATACAAACGCAATGATCCAACGCGTTCGTGTAGGGGACATGCGAGTGGGGGTATCCTATGCAATGAGTTTGCATAAGACCAGACCGCGAAATAGTAACCGCTAGCTGTAACACTGCTAACTAGTTAGGTTTCTATTTCACTAGGATGACCTAGGCAACTTAGTCTGAATCCTGAGTGGATCATGGACTCCTGTTCACTAGGGATTGTTCTTTGATTTGTATGGGTGAGAGTGGCCAATACGCCGAATCAATAAGCCTGCCATTTTGGGGACAAGACCGAGTGGGGAGTTGGGAACATAATCACACAAGATGGAATTCACTCCTTCCCGACTTTTGAGGAAGTAGATGAGTGTTCCCTTAAGTGGTGTCTCCGGGTCTTGAACAAAGGGCCCTACCCTCTCACTGGCCCGAGAGGGGTTTCTGTTTGATGGTTAGACCACAAACAGGTTGTTCATTAGAGGAGCACTAGTACTTAAGGAGGTAGAGGTAACTCAGGGGTAAAACGGTAATTTGACCCAGCTGAAGTTATGAACACTCGTGAAGGACTAACTTGCTGTTATTGGTCTATATCCATGGACACAGAATTATGTTTGCAGTGAGAAGAGTGCAACTGTGGGTCTTTAGTGGAGTGAACCCACAGTTAACGAAAATTGATTAATTGAATGAGTTTAGATGATTAAATCTCATATCGTTGGAGCTTCTGATCTGCAGATCCATTAGGTCCCACTGGTAGCTCATAAAGAAACTTGAGACAATTAAGTATAAATTGTAAATTGAAAGTGTTCAATTTATTTAGGGGAATCATATAATGTATATTGATACATTATAATATAAAAGTGTACGAAACTTTATAATATAATTTTGAATTAGTTTCAAAATTAATTATTGAGAGAAAATATTTGAAAGAGTTCAAATAATTAATATGAATTGGATTCATATTAATATAAATAATTTAATAAAACCCCTAAGATTAAATATATGATATTTAATTGGGTAATTAATTAATTATGTAATTAATTAATTATTTATTAAAATTAGATATGAATAAGATAATAGATTTGATTAGATCTTATTCATGACATTTTCCTATAAGGAAAAAGTCTTGGCCTTTTCTTATAAATAGGGCCCCTGGGGTGGGGTGAAAGACATGAAAGATTGGAATTCCGTAATTTCGTAAAACGGAAGCATGATTGGGACCGATTCGTAATTAATTTTGTTTAATCTAAAATACCAATACATTGGCAATAATCTTAGAACTATTCTAAATTTATATTAAGGCTAAGCATACAAAAATATTAGATTTAAATCTAATTTATATAGTAACAAATTACTTACTCTCGTTGACGACTCAGATTAAATTCACCAACTGCTTGTGAAATAATCACGAACTACTGCGAACACCACCACTATGGCTACACCGGTATGACTCTGAGACTTCTAGAGGCAGGTGATAACTTGCAAAAAGCAAGTTATTTCTCCTTTAAACTTAATCTAAAATAGGGCTAAAAAATATGTTGATGCGTTACTTCAACGCAATAAGAAAGGGAAATGCGTTGATTTTCTGCGAAAGGCTATATTTTTAGATGTGGTACGAAATTAGAAAAATATCAGGTGCTAACGCATTAATAATACAGTTGTTTGTTGCTCAAGTTTACCTCAGCGCAGACACACATACCAAGACAATCATGAACAGAAAAGATGGATGAATAGACCTTGCTGACACAACTGACGCGCAAGTAGCCAGAAGATAACTGAAGCAAGTTTTCTAAGTTCCTTGGAGAAGTCAGCTTCCTTTGCCTATAAATAGCATCCACCTGCTCAAAGGGGACGAGGAAGGAAGGAGAGACGAGAATCAAATAATCAAGGAGAAAGAGAAATCACAGAAAGTTGCGGTGGAGGGTAGACTTGCATTTGTGTTAGGGGAAGGAGTGAATTTCATCTTGTACTGTTATGTTCCCAGCCCCCATTCGGTCTTGCCCCTGAAATGGTAGGATTATTGAGTCGGCGAACTGGCCACTCTCACCCATACAAATCTAAGGATAATCCCTCCTGAAAAGGAGTTCATAACAAACTCAGGATTGAGATTGAGTTACCTAGTTCATCATATTGAAATAGAAACCTAAACTAGTCAACGACGTTACATCTAGTGGTTACTATTTCGCGGTCCGATCTTATGCAAACTCATTGCATAGGATACCCCCACCCGCATGTCTCCTACATGAATGCTTTGAATCATTGCATCTGTATCGGATACAAGGTGGGTCGTATCACATAGTGTCACCACGATAAGGTACCGAGCCTTATCCCTATACTATATACCATTTAGGTTGTTACTTGGACACTGATCCCCGTATGTCCCTACATACTAGTCCAAGTTTCATTTAAACAACCTTGGATCTTAGTTTATTGGATTTAGGGTTTATTAATGCAAACTCGATTATTCAATAACCAAATTACTAAATCACTCAAATAACGCTTTATTGAGATCGATTAATTTGTTTCATTTACTATCTACGAGTTTTAGGGCATAAAACCCAACATCAATTTCATTTTTCTCTCATTTGTTTCTATCTCTTTCTTCATCGTGTGAGTCTCTCTCTCTTCTTCTTCCTCTTTTCTCTTCTCTTGCTTTCTTCTTCATCCATTTTCTTTCTCGGTTTCTTTTCTCTCCTTATTCCCTCTTCTACCAGATTTCTCTTCGTCTACACCAAGTATTTTTTTCGCTCATTTTCTCTCTTCTTTTTTCCTTCCTCGTCAATCTCTCTTTCTCTCTTCGTCTTTCCTTTTTTCTCTTTTCTCGCCCCTTCTTTTCTCCTCAAAATGTAAGATAGTGTCCATCTCTCCTTTGAGAATCACCAAACCGACACACATCAACCGACCGATCGAGTATTTGGTCGGTCGATTTTCGTGAGCATTCCAAAACTATTAAGCACCGACCGATCGAAACCGAATAGGTCGGTTTTCGACGTCGGTTTTGGACCAACTCCGACCGAACGACGAACAACCCTACTCAGAGTCATACCGGTGTAACCATAGTGGTAGTGTTCGCTATAGTTCTTGATTATTTCACAAGTAGTTGGTGAATTTAATCTGAGTCGTCAACAAGAGTAAGTAACTTGTTACTATATAAATTAGATTTAGATCTAATATTTTTGTATGTTTAGCCTTAATATAAATCTAGAATAGTTCTAAGTTTTTTGCCAATGTATTGGTATTTTACATTAAACAAAATGAACTACGAATCGGTCCCAATTACGCTCCCGCTTTACGGAATTACGGAATTCCAATCCCTTCAGTTACTAGTATTCTCTCAATCCCCCGTGTCTTTCTTATCCCTTGAAAAACTCAAATTAAAGCTTGTCTGATGAAAGAATGGAGGCATTGTTTTGAATTTTTGTTTTTGTCATCATAACAATTGCAGAATAGCAACGAAAACAAAATCATCCTTGTGGCAAATTCGGACTTTTGAACTGCCGTATGCTACCCTGTGCCTTTTGATTGATTGCATTTGTCTGTACATCGAGACTTTGATCATCTATATGCTATCTGTTTTGGTCTCTGTTGAAAATTGCTTTGTTTCTGGTCTCCCAATTTTTCCGAAGCACGACCCACCGTGAATTCCATTATCCTTCGTTTGTTTTTTGTCTGATGTTTTACCTTTTTAGTATTTAATGAATTTTATTATTCTTTCAAAAAACAAAACCCTATACGTTTTTAAATTCGTCTTCTTCTTCTCTTTAAAAAAGTCATGTCGATATATTTATATTTTTTCTCTTTTCTTCACCACTTTCTGCCATTGCCGCTCCGGTCATTGGAATTTTCTGATTGAATTTTCATGTTAGCAACGAAGATTCCTACTGGACTCTGACCTTCGATCGCATTGTAGTCAGATTGTATTATATTCCAATTGATTGTTCGGGTCTTATTTTGAAAAAAATTATATTCTCTACGGGTAATTGATGGAAATGACCGAAAAAGTTTTTGGGTTATTGAAAACTGACCCTTCGTTAGAAATTAGTTTTTATGATCCTATTTGTCCTTCTCGGCTAGGATCATGATCGGTAAAAGCTTTTTCTTTTTGACCTCATTCTTCTTTGTGTGGTTAGAAAACTTCAAAATTCATCTTGGTACCACCTTTCTCTGTCATGATCACCATGAGGAAGGTGACCGGGAAACAAAAGTGAGACAATGCTGAAAATTTTTTCTCTAGGGCTTTTTCTTGGTCACCTTCCGTATCGGGATTGTGACCGAGATGGGTCATAAACACCATTTTTCAAGAAAAGGTGTCAATTTTCTATAACCCAAAACTTTTTGGGTAATTTCCATTACCCATTCCCTTCCTTAGTTTCAGAATTTGCAAGCCGCTTTTGAATTATATAAAGAGTTAAATAAAGTATTTGTACACTTTTGTTACCTTTTTTTTTTTTCTCAAACATTCTAAAATTCAGGGTTGGGCAGTCTCCAAGAGTTGAAGATTGATGCAACACTACCCATAGCTTGTATTATCGCAGCAACACCAACAAGGAGTTTACTATTTTCCTTGGTATTTCATTCGGTTATGCGATTCTTCGTCCCCTATTTCAACTCCTAGAACCTTCCTTTTCTGTTATTTTTATTTTTGCTCTTTCTGCAATGTTCTTATTAGCCCTAATATTTGTAAAAAGTTTGTTAATAGTGTGACGCCCCGTGCATTTTAGTGTAATTAATTCCATGTATCTAGTCGAATGAAATGTAATTTTTATTTTGGCTTGTACTTATGGAATTATTAAGTTTATACGAAGGCATTTGGGAATTAATTCTTGGAATTAAGCGTTATATTAATTTAAATGCCTCCCTAAAGCTCGATATATTTTTAAGCCAGCCAATGGTAAATGTTGAAATTTTCACGCTGAACGTGAAGAGAATTGGTTGGAAAAATCTATATTTGGAGTTCTATTCTTAGAAATCCTATTTTTGGGTTCTTTCTATTAGAAAAGGAATTAAGAAAAGAGAAAGGAAAGAAAAGGAAAAGAAAAAAAAAAGGATAAATTATAAAAAATACCCCTGAACTATGGGGTTTGTTTCAAAAATGTTCCTGAACTTTGAAAAGTTTCAAAAATACCCTTGCCCTTAATTTTTTTTTTTGATGGAAACCGTTAAAATTTCAGTTTAAAAAATACCATTGAACTTAAAAAATAAAAAATAAAAAATACCCTTATTGAAAAATGTTCTCTTTTTTCCTCATCTCACCGTAAATTCTAACGGTTTCCATCCAAAAATTAAGGACAAGAGTATTTTTGAAACATTTTCCAAAATTTAGAGGTATTTTTGAAATTTTTCAAAGTTCATCGGCATTTTTGGATAATTTTTATAATTTAGCAAAAAAAAAAAAAAAAAAAAAAAGAAGAAGAAGAAGAAGAAACCCTCCAAAACCCTCTCTCTCTCCCTCTTTTCTCTCTTGCGGGAAGGACCCCCACTCCCCGTGTGTTGCAGCCGCCGGCCGAAGACCCACTGCCGTCGCCGGAGTTGAAGCGCCGCTATCGTTGTGCCGTTGCAGTCAAGCCCGCCGCCGTCGCATCTCTCTCTCTCTGCGCGATTTCTTCTCCCTCGTTGTTCGTGGGTTTTATTTTTCGTTTCGCGCCGTCGCTTCGTGCCGCCGTCAGCCCAAGCGCTGCCGCCCTCGTGCCAGCAGTAGCGGCGTCGCCGGCGTCACCCATCCCGAGCCGCAGTCGCGTTTCCCCCTCTCTGTGGGTCTCGCTTCCGTGGGTTTTAGCCGTCGCCGTTCCTCTCCATTCCCGTCGTCGGCGTTTTCTTGAGCGTAATCTCTCATTTTTCAGCGCTGTGCAGTCTCATTTTGGACCCTAACGAAGCTGACCAGCGTGAAACTGGATCGTACTGGTAAAGTTTTTTGTTGAACTTTGTTCGGTTGCCCTTAAGTTTAATGCCCATGCTTAATCGGTTTCGATTTAAGTTGTCCATGACCCAAGTAGCGTTAATCTGAGTTACCCATTCCTTTTTGGGCATAAGGATTGAGTTTTCTGCTTTAATTTCTGTTCTGCAGTGCCGTCAAAGTGATCAATTGGGTTCGATTTGCCTCAACTTGGAGACCCGCTGTCTAAGGATCTTTTGGTGTGCTGTCCGGGATTAGCAAGACCCGTCTCAGCATGGTTTCGGGTTGTTTGGGTTATAAACCTTGTTTTTTGTGATTTAAAAGCATGCTAGGTTGTTACCCATTAATTGTTTAGGCTTTAAAAGCGTTGCCCAACTCCTGTAGGTCCTTAGAACGTGAAATTAATGAGTTTCTGCTTGCTGTTTATGGTCTAAAAGCATGCTAAATCGGTTCTCATTAATTATCTTAGGCCCTAGTAGCATTGCCTAATCTCTAGGAGTCCTTAGAATGCTAAATAAGCTTTATTTTCTTGCTGTCCAGTGGCTGAAAAGCCTTAATTATGGTCGTTCAATGAGTACCCATGACCATTGTGCCTTAGACATGATTGCCCATGGTCTAATGGCGTTGGGTTTTCACTTTTCAACTTGGGGAACTTTGAGTTGCTGTCCAGCAAGAGAATAGTCCATTCCTGATTGAATTAGGCCTTTCAAGCATGTTTAAAATATCTTTGGGTTGGTTCTAATTTAGTAGAGACTGTCTTGGATTGTTGAAATTGCTTTAGAAGCGTTGTAGGTAAGTAGTTTTGAGAGGTGGCAAGTTGTACGTGAAGTTGTTAATAGTTGAGTTTTGGTCAAATAAGGTTGCTTTCTCGAACATTTGGGTAATAAGGATGGTATTGCGGTGGACCCTGCAAGATAGGAGCAAATTGCAATGGGTTTGGACCCAGTTCAGTGGCTTAGGTTCACAATTGTCTTGGATTAACAAGTACTTTTGGAAGTTTTGAGTTTGTTTAGAAATTTTCCAGTGTGGTAGCATCATGGACTCCGTTGACTTGCTAGTTCAATAATATCTCTATCACCTAAAGCAAAGTTTGTAAGAAGAGTTCTAGAAGTTAAAGGAACAATACAGTCTCGATGTTGACTGTTTCTTGATGGTTCATGAGGTTTAGTGGTTGACAATGGACACTTCAAAAACAAAAGAAAATAATAATAAAAATAAATAAAGGACAAGGTTGTAGACTATTGCAATATGGAAAAGATGTGTTTACACCTTGCGTCAACCAAGGGGATATGAACCTAACTATTCCACTAATGATTTGGAACTCGCAGTCCAACATTAACTTTAAATATTTTAAGGCACTTTCCTTATGGAGAAAGTATCCAAACTCATAGGTCACAAGAGTTTGAAGTATTTCTTTGGACAAAAAGAGCTCAACTTAGGATAAAAGAAGTTCTGGAAACTGGTTAAGAATTGTGGTTGTGAATTCAATTTTTGCTATGGCAAGACAATTGTGGTATGAATTTAGCAAGAAAACAACAAGTATGGCATTCTTCATATCATCTAGTGACTCCGTTCCTAAGAGAGTTGATTGTTCAAAAATTGAGTTGGCAGTGGAAGACTTTTCAGCATTGTTGGCTCAAATTTAATGGTGTTAAGTTTGAAAGAGTGGATTGCAGATGACCAACAAGAGGATCTAGAATTGGTTAAACGCTTGCTGGGCTATCTTTTAAGACGAGATGATAGAAGGGGCACGTAACACACCTTTTTGATGCATCCTGACAGCATTGAGATGTGCAAGGACTCGAGAAGCTCTATTGATAGCATAATATGAAGAGAGAAATTGTTCAATCTATCGATACTTTCTTGTTCCTATCAGAATTGGAGTTCGTAAAGTTGACTTATAAAGTGTAAGGGTACTAGTATAGTATATAGGGAGATTAGTAGTAAGGGCATATTAGTAATTAGATAAGGAGTCTTGGTTATAAATAGGGAAGTTAGGGGTCTTAGAGAGGCGTGAAGAAATTTAGTAGTAGTGTCCATTGTGGGACTCTTGGGAGAGTGTTAGCCCTCTCGAAAGGCTATCGGTTATATTGTAGCGTCGTTTCGATATTGCAATATAATATATCAGTGTTTGGTATTTTTCTTAGTTTGGATTCCTAACATAAAGCTATCCAAAAGATCAGGACAGAGGATGTAAGTTGTCAGGGAAGGTAGGTAGATTGTGAAGGTGTCAGCACAAGGGTTTAAAGCTGGCATTGGTGACCTATGTTTCTTCCAGTATTGTCAGTGAAAGGGAGTTGTGTGGTGAGTAGATGGAATTGCTTAAACTCGAGTATATTGGACCACGTTGAGTTCATTGCGCACTTTGGTCTAGTTGTTCATTGGTTGTTTTGCCGCCAACGTTGTGGACACCACAACTTGATTCAAAAGTGTCATTGATAAAGAAGTATATTAATGACCTTTTCTTATTCATGGAATCTAGAACCCTTGCAGTTCGGTGGAAGTTGTTGTGTGAGCATAAACTATTAGGAAAGAGTGCCTTGGAAAGAAAGGTTGGAGTCAAGGTTAAGCGTTTAAAGTTTTTAGCAAAGCAACTTTCGAGGATGAAAGTTCTTAAGGTGGGGAGATTGTAACGCCCCGCGTTTCAAAATTAAGCTTTTCTGAGATATTTTGGATTCGTTATTGAGTTCAAATTGGACATTAAGTGGATGGAATCAAATCAATCAAATTATTAAGTGGTGGAATTTTGGTTTAGGAATCAAATTCTTGAACTCAATCCCATAAATATAGAAATTCAATTACCCAATGATTTCCTCAAATCTTTGAAAGGGAATTTGGGCTTTAATTGGAGTTTTAAAGATTAGGTTAAAGGATTAGAAAAGAAAAAAAAAAAAAAAGAAGAAAAGAAGCCCTAGAGACCTCCATCCCTTTCTCTCCTTCTCTTTCTCCTTCCCACGCCGTCACACCCCCCAATCGCCTCTTCTCCTTCTTCTTCGGCAGCGTGAGATCCCCTCCCCTCTTGATTTTTCTTCTCCCTGAGACCGTCGCTCGATCCCTTCAGCCACCCTTCGCGCCACTTCCTTCTTCTTCATCTTCGGCAAGCACCTCCGACGACATTGTGAGTAACGTGCATGAGACACCCAACCATGCGCGAGTTCAAGCCGTAGAGTCCTATTTAGGCATGATTTCTTCTTCCCCTTGGTGTGCTGCAGCGTAGTCGTTGATCGAAACCTCAAATCCATCACAGCTCCGGTAGCAACGACCTATGAACAACACCTTCGACGACCTTGTGCAACGACCCACGGATCGAGACCCATCTCTGCTCGTGGTTCAGACTTCCAGTGGGCTTCAACTCCGATTTTCCTCCTCTTCGGTAAGGCTTATCACTTGCTCATTCGTATTCGACCCTAGAACTAAGCTACCCATTCCCTAATAATGTCGATTGTGTAGGTTTAAGCTCTTGCGACCACCAAACAGCGAGGATTTGAGGCTGTTCAATGAGTGCCGATAAGTGGCAGCCAGTCGTAGGCCCTCTCATACGCAATTAAATGTTGTAAACTAGTCCTTAACCCTTGGGTATCATGTGTTTTAGGTTTGGGGCGATATTGGAGGGTTCAAATAAGGTTAAGGGTGAATTTCGAGCATTTTTCAGTAAGGGACAGCAAGTATAATGTTCTTTTCGAACGCATGTATGTGCTGGGAATTAGTTTCTAAGCTTTAGGTACTCTTTGTCGTAGGTTCCAGTTCGATTTTTGTGTGTTCGAATAAAATTCGAATCAACTTGTAGGGTCGTTCCTTGAACCGTTAAGAGTGTGTTAGGACTTTAGGTAAGTGGATTTCTACCGATTGGGTTTGTTTGCTTGTGTGATAGCATGCTGTGTGATATATCGATTTAGTTTTATTATGTGCTTTGCTGATGTATTGATGTTCATGAATTATCGTGAGTTTTGAGCATGTTGAATTATGTTAATCTATGATCTGGCCTTGCATCAGGTGTTGTTGTTGGTTTGTTATGTGTAATATGTTATTGTAATGTGGATCGACCTTGTGCGATAATTATGTTGTTCATGGTGATTGTTTGAGAAGCATGAATTTAATGCTGAGGAGCAGACCTGAGAAAGCATAGAATCTAGAATTTATTTAATTACTTTACTTCACTTGATTAGTTGTTGTTAGGAGAATTGGACTTTACGTTGGACTAGAGATACTTGTTTAAAAAGGGTGGTTTCTATTTACTTTGTTTTAATTGAGTTACTTAAATAGAATTGTTACGAGGCCTAACTTGTATTGACATTTTGGATTATTTGAAGTTACACTTTATCTACTGCTAGAGTTTTGCTATTGTGAAAGACCTTTAGATTTGATTTGAGTTATTTGGATGGATATGGTTACGAGGTCTGACTCGTAATCGCATTGTGACATTATTTAGTGTTATGCCATTGTTTTAGCAAGTTGTAAATTTTGGGTTACGTATAATTATGTTGTTATGCTGTCGATTTTTTCATAGCAGCGCAGTGGGTAATATGTCTAAGTTTGGTATTATACCTTAGCTAGTTTTTGAATTTTGGGGGCGATACAGGTTTGTTAATAGAATACATGATATTTACCCACACGAGCATCAATGGTTAACCCTAATATGTAACTCTTCTTGGCAGTTAGGCCTTGTAATTCCTTGAGCCGAGTCCCTACCTTTTCTTTTTTTCTTTTGTGCGCAAACTTACCTGCATACAGATTCCTGAATGATCTTGTCAATGATACAGTATCAACTCTGGTGGGAGCAACAGGTATGTCTGCACTGGAAGAGAAGCTAGATGAAATCCAACCTTCAACAAGGAATAATGATGAGTTGGAGGAAGAAGATGAAGGAGAGGAGGACCTTTCATGGTCATCAGACTCAGAGATTGGAGATGCATTGGATTGGTTGGATGCAAAGGAAGATACCGAAGCAGTAGATGGGGCTTTTTCACTCAATGCTAGGCGTCCTAATGCTCATGGTGGGCTTTATTCTCGTCATAATTCCTCGACTCTTCAACCTCTCTCAAATCGGAACCAAAAGTTTACCAATCACATTCGAGCTTCACCTCTTGCGGTATAATATACTTAATTTTCTAATTTTATACTGTTGGTTGTGTTTGCTCTTCTGCTAACAATATAAGGCAGCAAGTTGAACACTCATATTTGTTGGCATGAGCGTCAGATGCTTAGGCCCTTTATGAACCATCATTCTAGTATCTTCGTATAGTTTTATACTATACTTGTAATTTGTAAAGGAATATATGCTACTATTCTTAATTCTCATACTCATAGATTACCTGTAACTGAGGGACACCTTAATTATTTATCATGTTTGTTATTGTAGGAATGGGAAGGGAGATTTAATGTCGGAATGTCAAACTCTGTTACCACGGCTATTCGTGACAGTGTCAAGGAAATGGCTATAGGAAAAACGAAAACTACAGAGAAAGCTGATCGTGCAACTGTTGAACAGGTTGGTGATGATAATATTTTTTTGGTTTTTTCCTGAAACAATCCAATCCTCTATACACTATTTGTACTTATATTATCAAATGCATGATTACTGCAGGCAATAGATCCCAGAACTCGGATGGTTCTATTCAAGATGCTCAACCGAGGTGTTTTTCATGACATTAATGGCTGCATTTCTACTGGTAAAGAAGTATGTATCATATAATTTATTTTGTAAATAAAGCATAGAAATCTTTTGTAATCCTCTGTTTTTTATCTGGTAGGTTATTCTGAGTAATATTTATTCATTTGTCTTTCTCTGTCTTTCATCAACAGGCTAATGTCTATCATGCAACAAAATCTGATGGTCAAGAATTCGCTGTCAAAGTCTACAAAACTTCAGTTTTGGTGTTTAAGTATGTTTTAACGCTCTTTAGTTTGATTTGGAGTTCACTGCTTTGATTTTATTCAAAGATTTTACCAATTAGGTTCAACAATGATGATAAAAGCTTTTTTTTTTTTGTTAGTTTGATATGCATGAGAACGATTCTCGTAGTTTGGTTGTCCTTTCTATCTGTGAATCAAAATGTGCCTTTTGATTTTGTGATCATCTTTTCACTTTAGTTATATAGTAATGGGGGCTTTTATCAATTTCTTTTTCTAGACATAAAGGATGTCTACTATTGTTATAGAATTAACATGGATGTATCTTATTGTCATTTTGGAAGTCATTTATTGATTGCATTGATGTCCTGAGCTTAGTTTATCATGCTTGTCAACTTCAGGGATAGAGATCGCTATGTTCAAGGTGACTACCGTTTCAGACACGGATATTGCAGGCATAATCCCAGGAAAATGGTCAAGACATGGGCCGAGAAGGAAATGAGGAATTTGATGAGGTAATCATACCAACTCTTTGTAGTTCATAAAAGATATCTGTTGTGAGTTGTCTATAGCATCAAGTAAACAATTAATTGGGCTTCTGTTGATGTCAACTTAAGCTTAGTTTATCTGAGCTACAACCCATGGCGACCAACATTGTAAAATGAAATATCCTATGAAATTTTGGATTCTAATTTTTATAGTGTTATTTAGGTATTTGTTGGTTGAATTAGTTGAAGTGCATGCAAACTTTACACCCATGGAAAATTTTCTTTTACAATCTTTTGTATCATAGTATTTGTTACTTTGGACTTTCAGGCTAAAAGCTGCAGGGATTAGATGCCCGACTCCTCTCCTCTTGAGACTTCATGTGCTGGTCATGGAGTTCATTGGTATGTATACCCCATCCCTGAAATTATGTTTTAGAATAATCAATAATTGACATTTAATGTCAGATATTTACTTCATTATACAAGAATTTTCGGGCAGGGGTGAAAATTTCTTTTGTAATTATAGCTTGTTTTTGATTATTAACGATTGGCAGACTCTTTTGAAGTAGTTTTCGGGCAGGAGCAATCTCCTCCCATGCCCTTAGGCTGTTTCCACCCTTTTGTTAGATAATATATCTTGATGTTTCTTATAAAAAAATGCGAGATATTTACTTCATTTTTGGTTCGTTTGTGTCTTTAGGTTTGAACATGCAGATGTGCTTGATGTTGATGTCTTGTTTTCACTTCCTTTTTTAGGTTTAGTTGCAATTAGTTTTTTAGAAAACAATTAGAGTGCCCTTTTTTAGTTTTGATTTCCTTTCCTACCCTGCCCTGACAAAAGTTTCCATGATGTAAAAACGAAATACTCATAGAAGCTTACAGCAAGGAGCTTTAATTAAAAGGAGAGGCAATGGATAGAAAAACATCTTGAGCAAAGAGGAGTAAGAAAAGAAATATCGCGCTGAATGTTTGAAAGTTCCTTGTAAATATCTTGGAATAATAACCCATAGCTTGCTTTTGTTTTTTTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTTGACAAGATTCACACGGTAAGGAAAAAAACGGTTTTTCATTGAGAGAGGCATGAACCAACTAATACCAAAAATATTAAAAACCTTCCACCATTCAATTGAAAATCAAGCGAAAGAAGAACAAGTGACTTGTATTCTCTGTCTAATTTTAGCAAGGATGTAGTAGCTTGGATAGAGACCCTGGGAGGTTTCTTGTTTGTCTATCACTAGTACTTAGTTCTAGAAGGAAAGGATGTTCAAAATCATACTAATTTTGTATCGACTAATTTCACCTAGAAACTAGCACTGTATGACTGACATCTGTTTTTGGGTCACATCTTAAATCAAAGACACCATTGATTTTCTTTTTCCTCTTTTTTCTCTTTTGTAGGTAAGACAGGTTGGGCTGCACCTCGTCTTAAGGATGCTGCCTTGTCGTTGAACAGGCTACGTGAAAGTTATTTGGAGGTATGTTTGTATCTATCTCTGAATGTTACCACTTAGTCAAGTCGCAAGTGGTTTAATTATTGCTGCACTCAGATTTTCCTGCTTCCACTGTAATTTTTTTAAAACCCAGTAGCTAATCTTTAGAGGCCGTGAGTGCTGGAATTTTGGCTACTCTTTAGCGATAAATAAGTAATGATAGTTAGTTTCTCAAAAAATAAATAATCATAGTTGTACATAATTAATGTTAAAATGGAAAGTTGGTGCATGTAAACTCAGTTTTAGTTCTCAAGCTCTGTAGCCCCTAACTTCTGCAGCAGCAAGAAGAGGCTGATTAATGCCAGAGTTGCTTATTTAATGTTTTCATTCAAGAAAAAAATTTGTGGAAGAATGCTCTCTCCTCTCTCCTCTCTCCTCTACCCTCTCCCCATATAATTCCGGCCATGACCACTGCTATCTCTGCCACAGCTCAACCTTGGAACCACTCCACCAGATCTATCTACATTGATCGAAAAACTTTCTCCATTGAATTTGATGAACCTTCTAGGGGAAGCCGAGCAAAAATCACAGAGCATAGTAGAGCCTCCTCCCATTCCTTAACTTTGTCTTGGAAATCTCTCCATTGGCTAGCATCCTCCTTCAAAACTCTTGCCCATGAACCGTGCTCCTACAAATTCTCCTCCAAGATAAGAACTGATGACTATGTTCTCTGGTTGGAAAAACTCAGCAATAAGTATGGCTTCTTTGTGGAAATTAATCAACTGTTGAATTCAGGTGATCGACGTCGACTCCTTATACCATCTGAAGACAACAAGCAAGGTTGGTTCTCCTTTTTCTCCCTCATCTCTGATTACCCAGGAGAGGCTCATCGATCAACAAAATCATATAAAGATGTCTTCCAACAAAAGGTAAGTCATGTTGTCACCACTCACCCTTCATCATCTGTCCCTTCACCACAGCCTCTCGACAGTGAGATTATTGTTGTTCAACGATTCCATCAAAAGGATGATTGGCCTTCCATTCGGAACACCATTCTTGCCGGCATATCCCACCGTTGCTCCATCAATCCATTTCAAGATAATAAAGCTTTGTTACATGTATATGATCAACATATCGTGTCAAAACTTTGCAACAACAAGGATTGGTCCTCCATTGGCAAATACCGATTGAAGTTTTACCGATTGACTACCGACTCATTTTATCAAGACACTATGACTAATTCTTTTGGTGGATGGATTGAAGTGCTGCAACTTCCTTTACCTTTATGGACAGAACAAATTTTCAGATATATTGGTGATGTTTGTGGAGGCTTCACCGAAATATCCAACCACACCAGCAGGAAGCTAAATCTCACAGCGGCAAAGATTAAAATCCGGCAAAATTCCATCGGTTTCATCCCGGCCAGAATTAAGCTTCCTTCATCCCTTGCCGGCGGCGACGTTACAGTGGAAATCAAAGGGTTGACGGCCAGCTTTTTCAAATCAGCGAGATTTGAGGAATCCCCGTCATTTTCGGAACAAAATAGTTTAGAAATTAAGAGGAGTGAGAAATTGGATGGAAAAAATTTGAAACTACCAGAGGAAATCAAATCGCCTCCTAAAAATCAGGAATTCATTCCTATTTTGGCCGAAACTGCTTCTCCAAAGGATTTATCTCCTTGTCTGGTTTCTCCCCAAACTATATCACAGCCAAGAAGTATTTTGCAAGCGGCAGATCACTCCAATATATCTCCTAAAAAAAAGACAGCACACTCCAACAAAGGTAAATCTCCCCTCCACGTGGCCTCCCCCATTGAGGCAAAGAATCATACAAATTATTTTCTTCCAGTGGGACCCACCACTCTTGGTTTAGGAGAAAAGAAATCAACAGGCAACAAGATAATAGCTTCTGATACTGAGGCTTACTTATCAAGTCCAGCCAATGACAAATCCCCTCATACTTCGGTTTGTGACCCTACATCGCCTCGAAACTTTGACCTTGCAATTTTTGATGAGTTACATTTACCCGAGTCGGAACAAATTCCATTGGCAAGCTTACCTCCGACCTCCCACACTCCATCATCCCCCCATATTATTCCCTCTCCCACAAAAGATGTTACCCCACTACAACAACCATCTAGCTCTCCACCCGAACCATCACCCCTTTCCCTCCCAACATATCTCTGTCATTTAGCTCCAATGCTTAGTAAACATGGTTTATGCATCATGGCTCTTCCAACTGGCTCAATACCCAAACCGCCAACTAAGAAAACTAAAGCTACTACAAGGAAAAAGTCAAAACTTAAGAGAGAGGTACAAAATTTACAGAGTACAGTTCATTACGATAAATCGGCTACTTTGGCTTTATTGGAAGGAGAATCAATTGATCAATGAAGTTTATTTCATGGAATGTTAGAGGTTTGGGCTCTTGGAAAAAAAGAGCATTAATTAAGAAGACCATCCAACAACAAAATCCGAGCTTCGTGCTACTTCAAGAAACTAAAAAGACATCGGTTGATGGAAAATTTATTAAATCTATATGGAGTTCTTCTTGCATTGGTTGGGCTTCCCTTGATTCCATTGGAGCATCCGGAGGCATCCTTATTCTTTGGAGTGATCCTGATTTCACGATCAAAGAAGTTATTCAAGGTCACTTTTCAATCTCAATTCATGTTTTTATGGCTGACGGTTTTTCTTTTTGGCTTTCGGCTATTTATGGTCCTTCTAGGCGTGAGCACCGTGCAGATTTTTGGCAAGAACTCCATGATTTGGCTGGTTTAGGTGGTGATCGATGGATCCTTGGAGGGGATTTTAATGTTACTCGCTGGTCTTGGGAGAAATCTCATGGTCGAAATGTTACTAGGAGCATGCGCACTTTCAATCAATGGATTGCCAATTACCATCTCCTGGACATTCCACTACAAAATGGCTCTTACACTTGGTCTAGCTTTGGGGATGACATTGAATATCTCTCACTTCTGGATAGATTTTTATTAACAAATGATTGCCTTCACAAATTTGGGTCAGCAAATCTCCTTCGTCTTGATAGAGTCACATCAGATCACTACCCTTTAGCTCTTTCTTTTGGAGACATAGCTTGGGGGCCTTGTCCTTTCCGTTTTGACAATGCTTGGTTACATATTGAGTCCTTTCGTGAAGTTCTGAAAAACTGGTGGAACCAAAATCCTCTCCAAGGCTGGCCAGGGCATGGTTTTATGATGAAACTCAAGGGATTGAAAATGGAACTAAGAAAATGGAACATCACGAATCGTAATGATGTTTCCCAACTACCATCTCTTATTTCTCAATTGAAGAGTTTGGACAGTATTGGGGATGAGCACATTTTATCTACAGATCAGAAAGTACAGAGACGATTATTGAGGGAACAAATTGAAGACCAGACAGCCCGTGATCATATTGCTTGGCAACAAAGATGTAAGTTACAATGGCTCAAGGAAGGTGATGAAAATACTAGATTTTTTCATCGTATCATGGCTGCCCGTAAACGGAAAAACTCTATTCATGAGGTTCTTTCTAGAAATGGAATCAGTTTACTCACTGCTAGTGATATTGAGACGGAGTTCATTAGCTTCTACCAGTCTTTATTTACTAAAGACTATAATCACCGTTTTCTCCCAATAAACGTTGATTGGAGCCCTATCAGTGCAAATCAGTCAGCAGGCTTGGAATTGGCTTTTTCGGAGGAAGAAGTTTATCAGGCTGTAAAATCGCTAGGTACAAATAAATCTCCAGGTCCAGATGGTTTTACTGCCGAATTTTTTAAATACTCATGGCATATTATTAAATCGGATGTTATGACAATGATCAGAGATTTTTTCACCACAGGTATTATTAATGTGAGTCTGAATGAAACATATATTTGTCTAATCCCAAAGAAACTGGACTCCAAATCTGTCTCAGATTATCGGCCTATTAGTCTGATTCCATGTGCATACAAGATCATTGCTCGTGTTCTGTCCAACAGATTAAAGTTAGTTTTGCCATCTACTATCGCAGATAATCAATTGGCTTTTGTAGCCAACAGACAAATTTTAGATGCTTCTTTAATGGCCAATGAGTTAATTGATGATTGGACTATTTCTAATAAGAAAGGTGTGGTTCTTAAACTCGATCTGGAGAAGGCTTTTGATAAGATTGATTGGGACTTCTTGGATGCAGTTCTTCAAGCCAAAGGTTTTGGTTTGATTTGGAGAAAATGGATTCATGGTTGCATTTCAAGTGTTAACTACTCTATTATCATTAATGGTAGACCTAGGGGAAAAATTATTCCTTCTCGTGGTATTCGTCAAGGTGATCCTCTTTCTCCTTTTCTGTTCATATTGGTTTCTGATTGCCTCAGTCGTCTTTTATCTCACAGTGCAAGATTGGGTAGAATTATTTCTCATCCAATAGGTAACTCTCGTCTCTCATTGACACACTTGCAATTTGCGGACGATACTCTTCTTTTCTCCATCTATGATTCTAAAACATTGGATAATCTTTTTGAGATTATCAAACTCTTTGAGATGGCTTCTGGTTTGAACATCAATTTTGCTAAGAGTGAGCTTTTGGGGATTCACATTGCCGACTCAGATATGGAATGTTTGATAGCAAAATTTGGTTGTAAGCAGGGTTCATGGCCTTCCACATATCTGGGGCTTCCTTTGGGAGGCAGTTCGAAAGGTTCTCAGTTTTGGCAGCTTGTTATTGAAAGAATCCAACATAAGCTTCATAACTGGAAATACTCTTTTATCTCCAAAGGTGGTAGGCACACCCTCATCCAATCTACTCTCTCAAGTATGCCCACATATTATTTATCCTTATTTAAATTACCTTCTAAGGTTGCAAAATCTCTGGATAAGCTTATTCGAGATTTTTTTTGGGAGGGCTCCAGAGGCGATGGCGGCATGCATAATGTAAATTGGGAGACAACTCAGCTTCCAAAATTTATGGGTGGAGTAGGCATTGGAAATTTCCATCACCGTAATTTGGCTCTCTTATCAAAATGGATTTGGAGGTTTTTACATGAAGGTAATGCCCTTTGGCGCCAACTTATTATTGCTAAATATTATTTCTCAGAATCAACTTGTATTTGGCCTAAACATATTCAGAGAGGATCTTTTAAATCTCCTTGGCGCTTTATTTGTTCTACCATAGATCTTGTTGCTAGTCGTATTCAGAGACGGCTTGGAAATGGTTGTTCCACCCTTTTTTGGCATGATTCTTGGCTAAGTTGTGGAGTCTTGTCTGAGGCTTTCCCTCGTCTTTATAGATTATCTAATCGCTCGGAAGGTACAGTTGCTGACTTTTGGGTTTCATTGAATTCGGCTTGGGATTTGAGTCTTCGTCGAAATTTAAATGATTCGGAGACAAATGAGTGGGCTAGTCTCTCTCATCTGCTTTCTTCCATCAGAATTCGAGTTATTGATGACACTTGGTCTTGGCCTATTGATTCGTCTAATGCATTCACAGTTAAATCTCTTATGGGAGATATGGTTGGTGATTCTGACCCCACATCGAGCAAATTATATAATGTGGTGTGGAAAGACGTTTATCCAAAGAAGATCAAAATTTTTATCTGGGAGCTTAGTCTTGGAGCTATTAATACGTCTGATCGACTTCAAAGACGAATGCCTTATTTGCACCTTTCTCCATCCTGGTGTGTTATGTGTTGTTCTGATGCTGAAAATACTTGTCATCTATTTGTGCATTGCTCCTTTGCTTCCCGTTATTGGTCTACAATCTTCAATGCCTTTGAGTGGTCCTTGGCTCTACCAAACAACATTTATGATGTTCTTGCTTCCATTTTTGTGGGACATCCCTTCCATGGTGTGAAGAAGATCCTTTGGCTTGCTCTTAACCGGGTCTTCCTCTGGTTTCTTTGGGGCGAAAGGAATGGTCGAATTTTCAGGGATTCTTTCTCATCTTTTGAGAACTTTATGGATTTGATCCTTTTTTATGCTTTATATTGGTGCAAATGTAAACATCCATTTTCAGATTATAGTCTTTCTTCTTTAATTCTTAATTGGAGATCTTTCTTGTAATCACCTTCTTAGGTATTGGAGTTTTACTCCTTTTATTTCATTTATCAATGAAATTTGTTTCCCTTCAAAAAAAAAAAAAATTCAAGAAAAAAATTTGCTTGCATGGCTTCAATGTGGTAATATTTCTTTGTATTTTTCCAGATAATTATTACAATGCGGACCCTCTACCAGAAGTGCAAGTTAGTGCATGGAGATCTTAGTGAATATAACATACTTTATTTTGAGGTAACTGTGATTGTGGTCGGTTGGAAAAAACGACATTATGGACAAACTAATGCAAGAGTCTTCTTGCATGACAGGGTCACCTTTATATTATTGATGTTTCTCAAGCTGTTGATCTTGACCACCCTCATGCCCTCGATTTCCTTCGTGAAGATTGTCTGCATGTTTCTGTAAGTCTTCGTGCTCGAGGCTTATTCTGTTGGGTTCTTTTCTTTACTTCTTCATATGTTTTGAACTTTTGAAGTGAGTTTGAGAGGATATTTTGTATTGACAAACAGGATTTCTTTAAAAAACATGGTGTAGCTGTAATGACAATACGAGAACTATTTGATTTTATTGTTGATCCATGCCTCACTGATGAGACGATTGATAATTATCTAGAAGAGGTTTGATATCCAAGAATTATCTTTTTAATTATTACTATTATGATTAATTTTATAAAAGTTATGTTTTTTGACCATGTTGTACCAATTGCAGATGCAAGAAAAAATCTCGGCAAGAGGCGATATATCAGTGGAGGATGAGATTGCAGATTCTGTATTTGTACAGGTAACTTATATTTTTAGTTATCAACCGTGTTATTATAGGTTCATTTTATGATAGAATTGAAGGTTGATTCTTTGCGATCTTGATCGAGGATCGGTTTGATTAGAGTTCTTGGATTACTGCAATATAATTTTTAACCGATTCCGCTTCCCTTCCAGTCATATATTCCAAAAACACTGGATAGTGTCAAACATGCTGAGGAGGATGTAATACGTTTAACAAGTGGTCAGGACACTGAAGATATGTACTACAAGACCATTACAGGGTTAAAACAATCCCTTCCAAGAGTTGAACCTGTGTCAGAGCTAAAAGATGAGGAAGAACACAATGCAGACCCAATCGAACAATTCAAAAATTTAACCGGTTTGTCAAATTCTTCTGAGAAAGTGACTGAATCAACATTAGAGGAAGATGAAGAGAGCTCGAATGAGTCAGAAGGAGGACTAGGTTCTTTAACTGAGATGACGCAGGCGCCAGTTGACCGGAAAACTGCCAGAAAAGAGAACAAAAAGAAGGTGAAGGAAGAGAAGAGAGAGGCCCGCAAAAATAAGGTTCCAAAGGCTGTAAAGAAACGAAAGAAGAAGTTGGCCAAGTCCCATAAGTAA

mRNA sequence

CAGCAAGGAGTTTACTATTTTCCCTGTATCAACTCTGGTGGGAGCAACAGGTATGTCTGCACTGGAAGAGAAGCTAGATGAAATCCAACCTTCAACAAGGAATAATGATGAGTTGGAGGAAGAAGATGAAGGAGAGGAGGACCTTTCATGGTCATCAGACTCAGAGATTGGAGATGCATTGGATTGGTTGGATGCAAAGGAAGATACCGAAGCAGTAGATGGGGCTTTTTCACTCAATGCTAGGCGTCCTAATGCTCATGGTGGGCTTTATTCTCGTCATAATTCCTCGACTCTTCAACCTCTCTCAAATCGGAACCAAAAGTTTACCAATCACATTCGAGCTTCACCTCTTGCGGAATGGGAAGGGAGATTTAATGTCGGAATGTCAAACTCTGTTACCACGGCTATTCGTGACAGTGTCAAGGAAATGGCTATAGGAAAAACGAAAACTACAGAGAAAGCTGATCGTGCAACTGTTGAACAGGCAATAGATCCCAGAACTCGGATGGTTCTATTCAAGATGCTCAACCGAGGTGTTTTTCATGACATTAATGGCTGCATTTCTACTGGTAAAGAAGCTAATGTCTATCATGCAACAAAATCTGATGGTCAAGAATTCGCTGTCAAAGTCTACAAAACTTCAGTTTTGGTGTTTAAGGATAGAGATCGCTATGTTCAAGGTGACTACCGTTTCAGACACGGATATTGCAGGCATAATCCCAGGAAAATGGTCAAGACATGGGCCGAGAAGGAAATGAGGAATTTGATGAGGCTAAAAGCTGCAGGGATTAGATGCCCGACTCCTCTCCTCTTGAGACTTCATGTGCTGGTCATGGAGTTCATTGGTAAGACAGGTTGGGCTGCACCTCGTCTTAAGGATGCTGCCTTGTCGTTGAACAGGCTACGTGAAAGTTATTTGGAGATAATTATTACAATGCGGACCCTCTACCAGAAGTGCAAGTTAGTGCATGGAGATCTTAGTGAATATAACATACTTTATTTTGAGGGTCACCTTTATATTATTGATGTTTCTCAAGCTGTTGATCTTGACCACCCTCATGCCCTCGATTTCCTTCGTGAAGATTGTCTGCATGTTTCTGATTTCTTTAAAAAACATGGTGTAGCTGTAATGACAATACGAGAACTATTTGATTTTATTGTTGATCCATGCCTCACTGATGAGACGATTGATAATTATCTAGAAGAGATGCAAGAAAAAATCTCGGCAAGAGGCGATATATCAGTGGAGGATGAGATTGCAGATTCTGTATTTGTACAGTCATATATTCCAAAAACACTGGATAGTGTCAAACATGCTGAGGAGGATGTAATACGTTTAACAAGTGGTCAGGACACTGAAGATATGTACTACAAGACCATTACAGGGTTAAAACAATCCCTTCCAAGAGTTGAACCTGTGTCAGAGCTAAAAGATGAGGAAGAACACAATGCAGACCCAATCGAACAATTCAAAAATTTAACCGGTTTGTCAAATTCTTCTGAGAAAGTGACTGAATCAACATTAGAGGAAGATGAAGAGAGCTCGAATGAGTCAGAAGGAGGACTAGGTTCTTTAACTGAGATGACGCAGGCGCCAGTTGACCGGAAAACTGCCAGAAAAGAGAACAAAAAGAAGGTGAAGGAAGAGAAGAGAGAGGCCCGCAAAAATAAGGTTCCAAAGGCTGTAAAGAAACGAAAGAAGAAGTTGGCCAAGTCCCATAAGTAA

Coding sequence (CDS)

ATGTCTGCACTGGAAGAGAAGCTAGATGAAATCCAACCTTCAACAAGGAATAATGATGAGTTGGAGGAAGAAGATGAAGGAGAGGAGGACCTTTCATGGTCATCAGACTCAGAGATTGGAGATGCATTGGATTGGTTGGATGCAAAGGAAGATACCGAAGCAGTAGATGGGGCTTTTTCACTCAATGCTAGGCGTCCTAATGCTCATGGTGGGCTTTATTCTCGTCATAATTCCTCGACTCTTCAACCTCTCTCAAATCGGAACCAAAAGTTTACCAATCACATTCGAGCTTCACCTCTTGCGGAATGGGAAGGGAGATTTAATGTCGGAATGTCAAACTCTGTTACCACGGCTATTCGTGACAGTGTCAAGGAAATGGCTATAGGAAAAACGAAAACTACAGAGAAAGCTGATCGTGCAACTGTTGAACAGGCAATAGATCCCAGAACTCGGATGGTTCTATTCAAGATGCTCAACCGAGGTGTTTTTCATGACATTAATGGCTGCATTTCTACTGGTAAAGAAGCTAATGTCTATCATGCAACAAAATCTGATGGTCAAGAATTCGCTGTCAAAGTCTACAAAACTTCAGTTTTGGTGTTTAAGGATAGAGATCGCTATGTTCAAGGTGACTACCGTTTCAGACACGGATATTGCAGGCATAATCCCAGGAAAATGGTCAAGACATGGGCCGAGAAGGAAATGAGGAATTTGATGAGGCTAAAAGCTGCAGGGATTAGATGCCCGACTCCTCTCCTCTTGAGACTTCATGTGCTGGTCATGGAGTTCATTGGTAAGACAGGTTGGGCTGCACCTCGTCTTAAGGATGCTGCCTTGTCGTTGAACAGGCTACGTGAAAGTTATTTGGAGATAATTATTACAATGCGGACCCTCTACCAGAAGTGCAAGTTAGTGCATGGAGATCTTAGTGAATATAACATACTTTATTTTGAGGGTCACCTTTATATTATTGATGTTTCTCAAGCTGTTGATCTTGACCACCCTCATGCCCTCGATTTCCTTCGTGAAGATTGTCTGCATGTTTCTGATTTCTTTAAAAAACATGGTGTAGCTGTAATGACAATACGAGAACTATTTGATTTTATTGTTGATCCATGCCTCACTGATGAGACGATTGATAATTATCTAGAAGAGATGCAAGAAAAAATCTCGGCAAGAGGCGATATATCAGTGGAGGATGAGATTGCAGATTCTGTATTTGTACAGTCATATATTCCAAAAACACTGGATAGTGTCAAACATGCTGAGGAGGATGTAATACGTTTAACAAGTGGTCAGGACACTGAAGATATGTACTACAAGACCATTACAGGGTTAAAACAATCCCTTCCAAGAGTTGAACCTGTGTCAGAGCTAAAAGATGAGGAAGAACACAATGCAGACCCAATCGAACAATTCAAAAATTTAACCGGTTTGTCAAATTCTTCTGAGAAAGTGACTGAATCAACATTAGAGGAAGATGAAGAGAGCTCGAATGAGTCAGAAGGAGGACTAGGTTCTTTAACTGAGATGACGCAGGCGCCAGTTGACCGGAAAACTGCCAGAAAAGAGAACAAAAAGAAGGTGAAGGAAGAGAAGAGAGAGGCCCGCAAAAATAAGGTTCCAAAGGCTGTAAAGAAACGAAAGAAGAAGTTGGCCAAGTCCCATAAGTAA

Protein sequence

MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFSLNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIRDSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMRLKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQKCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVMTIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVKHAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLSNSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNKVPKAVKKRKKKLAKSHK
Homology
BLAST of Lcy13g011650 vs. ExPASy Swiss-Prot
Match: Q54VD8 (Serine/threonine-protein kinase rio1 OS=Dictyostelium discoideum OX=44689 GN=rio1 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.3e-113
Identity = 246/560 (43.93%), Postives = 346/560 (61.79%), Query Frame = 0

Query: 6   EKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFSLNARR 65
           +K+  ++   R N +L E  + +E+  +  D E    +++     D E  D    + +  
Sbjct: 30  KKIQTLEVPLRRNGDLIEFSDSDEEYYYDDDYE---EIEYDSDLSDEENEDRILGIRSSI 89

Query: 66  PNAHGGLYSRHNSSTLQPLSNR-NQKFTNHIRASPLAEWEGRFNV-------GMSNSVTT 125
           P           + TLQP +    QK+ N I  + +A       V        + NSV  
Sbjct: 90  P----------LNKTLQPKAQALEQKYQNKINVNSIANSISSKRVIDDDSHRALPNSVQN 149

Query: 126 AIRDSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEAN 185
           +I++  K+      +  +K DRAT EQ +DPRTR++LFKM+N+G F +INGCISTGKEAN
Sbjct: 150 SIKEIKKKDDKQGVRIVDKEDRATTEQVLDPRTRLMLFKMINKGAFSEINGCISTGKEAN 209

Query: 186 VYHATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRN 245
           VYHA   + +E AVKVYKTS+LVFKDRDRYV G++RFR GY +HNPRKMVK WAEKE RN
Sbjct: 210 VYHAFTPNEEERAVKVYKTSILVFKDRDRYVTGEFRFRRGYSKHNPRKMVKVWAEKEFRN 269

Query: 246 LMRLKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRT 305
           L RLK AGI CPTPL+LR H+LVM FIGK G+AAPRLKDA +S  +    YL+ I  MRT
Sbjct: 270 LTRLKNAGIPCPTPLILRNHILVMTFIGKDGYAAPRLKDATVSQEKFGVIYLDCIKMMRT 329

Query: 306 LYQKCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGV 365
           L+ KC+LVH DLSEYN+LY++  LYIIDVSQ+V+ DHPH+LDFLR DC +V+DFF+K  V
Sbjct: 330 LFHKCRLVHADLSEYNMLYYKNQLYIIDVSQSVEHDHPHSLDFLRMDCSNVTDFFRKKEV 389

Query: 366 AVMTIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLD 425
             M I+ELF+FI D  +T++ ID YLE+M EKI +RG+ + E +I + VF  +YIP+TLD
Sbjct: 390 NTMFIQELFEFITDLTITEDNIDQYLEKMLEKIQSRGETTDEQKIQEEVFRNAYIPRTLD 449

Query: 426 SVKHAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLT 485
            +   + D+ ++  G+   D++Y+ +TGL + L  ++  ++L ++   N D  +      
Sbjct: 450 QIIDLDRDMEKIERGEG-RDIFYQNLTGLSKDLQNIKSKNDLINDSNENKDSDD------ 509

Query: 486 GLSNSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTAR------KENKKKVKE 545
              +SS+   +S  + D +  NE +  +  L  +   P+ +   R      K+ KK VKE
Sbjct: 510 ---SSSDSSEDSDDDSDSDQLNEDDEKIRKLVVID--PITKMEIRLEDMPKKDRKKFVKE 564

Query: 546 EKREARKNKVPKAVKKRKKK 552
             +E RK K+PK +KK  KK
Sbjct: 570 MNKERRKTKIPKHIKKLTKK 564

BLAST of Lcy13g011650 vs. ExPASy Swiss-Prot
Match: Q9BRS2 (Serine/threonine-protein kinase RIO1 OS=Homo sapiens OX=9606 GN=RIOK1 PE=1 SV=2)

HSP 1 Score: 402.1 bits (1032), Expect = 1.0e-110
Identity = 250/566 (44.17%), Postives = 337/566 (59.54%), Query Frame = 0

Query: 9   DEIQPSTRNNDELEEEDEGEE-----DLSWSSDSEIGDALDWLDAKEDTEAVDGAFSLNA 68
           +++Q +   N E E EDE EE     D  W  D  +G            +   G      
Sbjct: 41  EDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVG------------KLAKGYVWNGG 100

Query: 69  RRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIRDSV 128
             P A+       ++    P     +KF N I    L          +++SV   + +  
Sbjct: 101 SNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKL---------NVTDSVINKVTEKS 160

Query: 129 KEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATK 188
           ++      +  +KADRATVEQ +DPRTRM+LFKML RG+  +INGCISTGKEANVYHA+ 
Sbjct: 161 RQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHAST 220

Query: 189 SDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMRLKA 248
           ++G+  A+K+YKTS+LVFKDRD+YV G++RFRHGYC+ NPRKMVKTWAEKEMRNL+RL  
Sbjct: 221 ANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNT 280

Query: 249 AGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQKCK 308
           A I CP P++LR HVLVM FIGK    AP LK+  LS ++ RE YL++I  MR +YQ  +
Sbjct: 281 AEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDAR 340

Query: 309 LVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVMTIR 368
           LVH DLSE+N+LY  G +YIIDVSQ+V+ DHPHAL+FLR+DC +V+DFF +H VAVMT+R
Sbjct: 341 LVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVR 400

Query: 369 ELFDFIVDPCLTDETIDNYLEEMQEKISAR--GDISVEDEIADSVFVQSYIPKTLDSVKH 428
           ELF+F+ DP +T E +D YL +  E  S R   + S +D + + VF ++YIP+TL+ VK+
Sbjct: 401 ELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKN 460

Query: 429 AEE--DVIRLTSGQD------TEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQF 488
            E   D+I     +D       +++ Y+T+TGLK+ L  V+ V  L              
Sbjct: 461 YERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPAL-------------- 520

Query: 489 KNLTGLSNSSEKVTESTLEE--DEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKE 548
                L N  E+ T S  E+    E S+      G      +   D    +KE KK VKE
Sbjct: 521 -----LENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKE 566

Query: 549 EKREARKNKVPKAVKKRKKKLAKSHK 558
            +RE RKNK+PK VKKRK+K AK+ K
Sbjct: 581 AQREKRKNKIPKHVKKRKEKTAKTKK 566

BLAST of Lcy13g011650 vs. ExPASy Swiss-Prot
Match: Q922Q2 (Serine/threonine-protein kinase RIO1 OS=Mus musculus OX=10090 GN=Riok1 PE=1 SV=2)

HSP 1 Score: 394.8 bits (1013), Expect = 1.6e-108
Identity = 250/575 (43.48%), Postives = 346/575 (60.17%), Query Frame = 0

Query: 4   LEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIG---DALDWLDAKEDTEAVDGAFS 63
           L + L       + + E +EED+ ++D  W  D   G       W          +G  +
Sbjct: 40  LLKSLQNAAAEVKGDAETDEEDDYDDDDDWYLDDATGKLTKGCTW----------NGGSN 99

Query: 64  LNARRPNAHGGLYSRHNSSTLQ-PLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAI 123
             A R        S +NS+ +  P+    +KF N I  + L          +++SVT  +
Sbjct: 100 YQANRQT------SNYNSAKMSTPIDKSLRKFENKINLNKL---------NVTDSVTNKV 159

Query: 124 RDSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVY 183
              +++      +  +KADRATVEQ +DPRTRM+LFK+L++    +I+GCISTGKEANVY
Sbjct: 160 TVKLRQKEAESYRIKDKADRATVEQVLDPRTRMILFKLLHKDHISEIHGCISTGKEANVY 219

Query: 184 HATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLM 243
           +A+   G+  A+K+YKTS+L+FKDRD+YV G++RFR GYC+ NPRKMV+TWAEKEMRNL 
Sbjct: 220 YASTPSGESRAIKIYKTSILMFKDRDKYVTGEFRFRRGYCKGNPRKMVRTWAEKEMRNLC 279

Query: 244 RLKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLY 303
           RLK A I CP P+ LR HVL+M FIGK    AP LK+  LS ++ RE YL++I  MR +Y
Sbjct: 280 RLKTANIPCPEPIRLRSHVLLMGFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRKMY 339

Query: 304 QKCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAV 363
           Q  +LVH DLSE+N+LY  G +YIIDVSQ+V+ DHPHAL+FLR+DC +V+DFF KH VAV
Sbjct: 340 QDARLVHADLSEFNMLYHGGDVYIIDVSQSVEHDHPHALEFLRKDCTNVNDFFSKHAVAV 399

Query: 364 MTIRELFDFIVDPCLTDETIDNYLEEMQEKISAR--GDISVEDEIADSVFVQSYIPKTLD 423
           MT+RELFDF+ DP +T + +D YLE+  E  S R   + + +D + + VF Q+YIP+TL+
Sbjct: 400 MTVRELFDFVTDPSITADNMDAYLEKAMEIASQRTKEEKTSQDHVDEEVFKQAYIPRTLN 459

Query: 424 SVKHAEEDV----------IRLTSGQDTEDMYYKTITGLKQSLPRVEPV-----SELKDE 483
            VK+ E DV          + L + QD  ++ Y+T+ GLK+ L  V+ V     SE+K+E
Sbjct: 460 EVKNYERDVDIMMRLKEEDMALNTQQD--NILYQTVMGLKKDLSGVQKVPALLESEVKEE 519

Query: 484 EEHNADPIEQFKNLTGLSNSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTAR 543
               +D         G S  S+ V+E           E E   G    +    +D    +
Sbjct: 520 TCFGSDD-------AGGSECSDTVSE-----------EQEDQAGCRNHIADPDID----K 565

Query: 544 KENKKKVKEEKREARKNKVPKAVKKRKKKLAKSHK 558
           KE KK VKE +RE RKNK+PK VKKRK+K AK+ K
Sbjct: 580 KERKKMVKEAQREKRKNKIPKHVKKRKEKTAKAKK 565

BLAST of Lcy13g011650 vs. ExPASy Swiss-Prot
Match: O44959 (Serine/threonine-protein kinase RIO1 OS=Caenorhabditis elegans OX=6239 GN=riok-1 PE=2 SV=5)

HSP 1 Score: 335.5 bits (859), Expect = 1.2e-90
Identity = 189/419 (45.11%), Postives = 268/419 (63.96%), Query Frame = 0

Query: 130 KTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKSDGQEF 189
           + +  ++ADRATVEQ +DPRTR+VLF++L RG   +I+GCISTGKEANVYHAT +D  + 
Sbjct: 108 RKRVKDRADRATVEQVLDPRTRLVLFRLLQRGTLLNIDGCISTGKEANVYHATGTD-NDL 167

Query: 190 AVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMRLKAAGIRCP 249
           A+K+YKTS+L FKDR+RYV G++R+RHGYC+ NPRKMV  WAEKEMRNL R+   G+  P
Sbjct: 168 AIKIYKTSILTFKDRERYVTGEFRYRHGYCKSNPRKMVAVWAEKEMRNLARMHEVGLPVP 227

Query: 250 TPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQKCKLVHGDL 309
            P LL+ HVLVM+F+GK GW AP LK+A LS       Y+ ++  MR LY++CKLVH DL
Sbjct: 228 KPHLLKGHVLVMDFLGKDGWPAPLLKNANLSQEDAEPMYVGLVRDMRRLYRECKLVHADL 287

Query: 310 SEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVMTIRELFDFI 369
           SE+N+L  +G L+IIDVSQ+V+ DHPHAL+FLR DC +V+ FF++ GV V+++R LF+ I
Sbjct: 288 SEFNMLVHDGKLWIIDVSQSVEQDHPHALEFLRMDCNNVNKFFRELGVPVLSVRRLFEVI 347

Query: 370 VDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVKHAEEDVIRL 429
           VDP ++ + ++  +EE +  +++      ED   DS+F+ ++IP  L+ V H E D    
Sbjct: 348 VDPLMSSKEMETIIEEERVLVNS------ED---DSLFMNAFIPHKLEHVLHFERDGKLA 407

Query: 430 TSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLSNSSEKVTES 489
             G +  + +   ++ +     + +   E  D+ + N D             + +K  + 
Sbjct: 408 KEGVEANNPFQNIVSKIDL---KGDGFGEEHDDSDDNDD-----------EENGKKSRKK 467

Query: 490 TLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNKVPKAVKKR 549
             E  EE   E E  +   T   +   +    RKE K  VKEEKRE RK K+PK +KKR
Sbjct: 468 RAEPTEEEIQEKERKIAMHTRNREETAEE---RKERKAAVKEEKREQRKEKIPKHLKKR 499

BLAST of Lcy13g011650 vs. ExPASy Swiss-Prot
Match: O42650 (Serine/threonine-protein kinase rio1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rio1 PE=3 SV=2)

HSP 1 Score: 318.2 bits (814), Expect = 1.9e-85
Identity = 209/540 (38.70%), Postives = 303/540 (56.11%), Query Frame = 0

Query: 16  RNNDELEEEDEGEEDLSWSSDSEI--GDALDWLDAKEDTEAVDGAFSLNARRPNAHGGLY 75
           +N+ +  EE+    D S  SD++    D +DW  A  +  A    F  N+ + N+     
Sbjct: 6   KNDAQTNEEESEYSDFSDGSDNDYFREDDIDWNQASSNYSARQENFGNNSSKINS----- 65

Query: 76  SRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIRDSVKEMAIGKTKT 135
              + STL        ++ N+I+ +   E E                         K+  
Sbjct: 66  VNDHVSTL-------SRYVNNIKLNDRFEAE------------------------DKSSI 125

Query: 136 TEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKSDGQEFAVKV 195
            +K+DRAT EQ +DPRTRM+L K++N G   +INGCISTGKEANVYHAT  DG+ FA+K+
Sbjct: 126 KDKSDRATSEQVLDPRTRMILLKLINNGTISEINGCISTGKEANVYHATNEDGKHFAIKI 185

Query: 196 YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMRLKAAGIRCPTPLL 255
           YKTS+LVFKDRDRYV G++RFRHGY + NPRKMV+ WAEKE+RNL R+ AAGI CP P+L
Sbjct: 186 YKTSILVFKDRDRYVSGEFRFRHGYNKRNPRKMVRLWAEKEIRNLKRVAAAGIPCPEPIL 245

Query: 256 LRLHVLVMEFIG-KTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQKCKLVHGDLSEY 315
           L+ HVL+M F+G K GWA P+LKD  ++     + Y  +   MR L+  C LVH DLSEY
Sbjct: 246 LKQHVLLMSFLGDKKGWAYPKLKDIDMTPGEATKLYQLVARNMRILFHVCHLVHADLSEY 305

Query: 316 NILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVMTIRELFDFIVDP 375
           N+LY +G +Y IDVSQ+V+ DHP ++DFLR D L++S FF++     +++ +LF FI + 
Sbjct: 306 NLLYHKGKVYFIDVSQSVEHDHPQSIDFLRMDILNISTFFRRLNAGCLSLPQLFKFITEE 365

Query: 376 CLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVKHAEEDVIRLTSG 435
              ++      E M+ +++A  +     E  +  F+++Y+P+TLD V   + D   + +G
Sbjct: 366 GSCEK------EAMKTRLNAIYEEEPTTEEYEEEFLKTYVPRTLDEVYDIDRDTEIVNAG 425

Query: 436 QDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS-NSSEKVTESTL 495
                +Y   +    Q L       +L D  ++  D I    N T  S + +  ++    
Sbjct: 426 GVNSLVYKHLLNTDFQKL-------DLNDTTKNQNDQI--LPNETSESDDDANSISSMEN 485

Query: 496 EEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNKVPKAVKKRKKK 552
           EE+  S ++S    G      +   + K AR   KKK KE+K E RK+K+PK  KKRK K
Sbjct: 486 EEERTSDSKSSAKQGKGNGRAKETPEEKRAR---KKKTKEDKAEKRKSKIPKYEKKRKLK 491

BLAST of Lcy13g011650 vs. ExPASy TrEMBL
Match: A0A5D3BH21 (Serine/threonine-protein kinase RIO1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002840 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.2e-289
Identity = 521/557 (93.54%), Postives = 533/557 (95.69%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           M A+EEK+DEI+PST NNDE  EEDEGEEDLSWSSDSEIGDALDWLDAKED E VDGAFS
Sbjct: 6   MPAVEEKVDEIEPSTMNNDESVEEDEGEEDLSWSSDSEIGDALDWLDAKEDRETVDGAFS 65

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR
Sbjct: 66  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 125

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 126 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 185

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFA+K+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 186 ATKSDGQEFAIKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 245

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLR+HVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ
Sbjct: 246 LKAAGIRCPTPLLLRMHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 305

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 306 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 365

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDETIDNYLEE+QEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 366 TIRELFDFIVDPCLTDETIDNYLEEIQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 425

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQDTEDMYYKTITGLKQ+LP  +P S+ KDEEEHN D I   +N T LS
Sbjct: 426 HAEEDVIRLTSGQDTEDMYYKTITGLKQALPIAQPTSDQKDEEEHNTDMIGPSENTTSLS 485

Query: 481 NSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNK 540
           NS EK TEST EEDEESSNESEG LGS TE TQ PVDRKTARKENKKKVKEEKREARKNK
Sbjct: 486 NSCEKETESTSEEDEESSNESEGELGSSTETTQTPVDRKTARKENKKKVKEEKREARKNK 545

Query: 541 VPKAVKKRKKKLAKSHK 558
           +PKAVKKRKKKLAKSHK
Sbjct: 546 IPKAVKKRKKKLAKSHK 562

BLAST of Lcy13g011650 vs. ExPASy TrEMBL
Match: A0A1S3ATK0 (Serine/threonine-protein kinase RIO1 OS=Cucumis melo OX=3656 GN=LOC103482696 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.2e-289
Identity = 521/557 (93.54%), Postives = 533/557 (95.69%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           M A+EEK+DEI+PST NNDE  EEDEGEEDLSWSSDSEIGDALDWLDAKED E VDGAFS
Sbjct: 6   MPAVEEKVDEIEPSTMNNDESVEEDEGEEDLSWSSDSEIGDALDWLDAKEDRETVDGAFS 65

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR
Sbjct: 66  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 125

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 126 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 185

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFA+K+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 186 ATKSDGQEFAIKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 245

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLR+HVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ
Sbjct: 246 LKAAGIRCPTPLLLRMHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 305

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 306 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 365

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDETIDNYLEE+QEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 366 TIRELFDFIVDPCLTDETIDNYLEEIQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 425

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQDTEDMYYKTITGLKQ+LP  +P S+ KDEEEHN D I   +N T LS
Sbjct: 426 HAEEDVIRLTSGQDTEDMYYKTITGLKQALPIAQPTSDQKDEEEHNTDMIGPSENTTSLS 485

Query: 481 NSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNK 540
           NS EK TEST EEDEESSNESEG LGS TE TQ PVDRKTARKENKKKVKEEKREARKNK
Sbjct: 486 NSCEKETESTSEEDEESSNESEGELGSSTETTQTPVDRKTARKENKKKVKEEKREARKNK 545

Query: 541 VPKAVKKRKKKLAKSHK 558
           +PKAVKKRKKKLAKSHK
Sbjct: 546 IPKAVKKRKKKLAKSHK 562

BLAST of Lcy13g011650 vs. ExPASy TrEMBL
Match: A0A0A0LQN2 (Serine/threonine-protein kinase RIO1 OS=Cucumis sativus OX=3659 GN=Csa_1G043280 PE=3 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 2.8e-289
Identity = 518/557 (93.00%), Postives = 534/557 (95.87%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           M A+EEK+DE++PS  NNDE  EEDEGEEDLSWSSDSEIGDALDWLDAKED E VDG FS
Sbjct: 6   MPAVEEKVDEVEPSIMNNDESVEEDEGEEDLSWSSDSEIGDALDWLDAKEDRETVDGTFS 65

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR
Sbjct: 66  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 125

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 126 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 185

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFA+K+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 186 ATKSDGQEFAIKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 245

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLR+HVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ
Sbjct: 246 LKAAGIRCPTPLLLRMHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 305

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQ+VDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 306 KCKLVHGDLSEYNILYFEGHLYIIDVSQSVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 365

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDET+DNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 366 TIRELFDFIVDPCLTDETMDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 425

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQDTEDMYYKTITGLKQ+LP  +P S+LKDEEEHNAD I+  +++TGLS
Sbjct: 426 HAEEDVIRLTSGQDTEDMYYKTITGLKQALPMAQPTSDLKDEEEHNADMIKPSESITGLS 485

Query: 481 NSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNK 540
           NS EK TEST EEDEESSNESEG  GS TE TQ PVDRK ARKENKKKVKEEKREARKNK
Sbjct: 486 NSCEKATESTSEEDEESSNESEGEPGSSTETTQTPVDRKAARKENKKKVKEEKREARKNK 545

Query: 541 VPKAVKKRKKKLAKSHK 558
           VPKAVKKRKKKLAKSHK
Sbjct: 546 VPKAVKKRKKKLAKSHK 562

BLAST of Lcy13g011650 vs. ExPASy TrEMBL
Match: A0A6J1JYS0 (Serine/threonine-protein kinase RIO1 OS=Cucurbita maxima OX=3661 GN=LOC111490077 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.9e-278
Identity = 509/560 (90.89%), Postives = 528/560 (94.29%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           MSA+EEKL+EI+PSTRNN+E  EED+ EEDLSWSSDSEIG+ALDWLDAKED EA DGAFS
Sbjct: 1   MSAVEEKLEEIEPSTRNNEEPVEEDDEEEDLSWSSDSEIGEALDWLDAKEDREA-DGAFS 60

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPL EWEGRFNVGMSNSVTTAIR
Sbjct: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLGEWEGRFNVGMSNSVTTAIR 120

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFAVK+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 181 ATKSDGQEFAVKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLE+IITMRTLYQ
Sbjct: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEVIITMRTLYQ 300

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQ+VDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQSVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDETID+YLEE+QEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 361 TIRELFDFIVDPCLTDETIDSYLEELQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQ+TEDMYYKTITGLKQ+LPRV+P SEL    EH+ DPIEQ +NLT LS
Sbjct: 421 HAEEDVIRLTSGQNTEDMYYKTITGLKQALPRVQPTSEL----EHDTDPIEQSENLTSLS 480

Query: 481 NSSEKVTESTLEEDEESSNESE---GGLGSLTEMTQAPVDRKTARKENKKKVKEEKREAR 540
           NS EK TES  E DEESS+ESE   G   S    TQ P +RK ARKENKKKVKEEKRE+R
Sbjct: 481 NSCEKETESMSEVDEESSDESEEEDGPSSSTETTTQTPAERKAARKENKKKVKEEKRESR 540

Query: 541 KNKVPKAVKKRKKKLAKSHK 558
           KNKVPKAVKKRKKKLAKSHK
Sbjct: 541 KNKVPKAVKKRKKKLAKSHK 555

BLAST of Lcy13g011650 vs. ExPASy TrEMBL
Match: A0A6J1K0V1 (Serine/threonine-protein kinase RIO1 OS=Cucurbita maxima OX=3661 GN=LOC111490077 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.9e-278
Identity = 509/560 (90.89%), Postives = 528/560 (94.29%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           MSA+EEKL+EI+PSTRNN+E  EED+ EEDLSWSSDSEIG+ALDWLDAKED EA DGAFS
Sbjct: 21  MSAVEEKLEEIEPSTRNNEEPVEEDDEEEDLSWSSDSEIGEALDWLDAKEDREA-DGAFS 80

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPL EWEGRFNVGMSNSVTTAIR
Sbjct: 81  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLGEWEGRFNVGMSNSVTTAIR 140

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 141 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 200

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFAVK+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 201 ATKSDGQEFAVKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 260

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLE+IITMRTLYQ
Sbjct: 261 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEVIITMRTLYQ 320

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQ+VDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 321 KCKLVHGDLSEYNILYFEGHLYIIDVSQSVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 380

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDETID+YLEE+QEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 381 TIRELFDFIVDPCLTDETIDSYLEELQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 440

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQ+TEDMYYKTITGLKQ+LPRV+P SEL    EH+ DPIEQ +NLT LS
Sbjct: 441 HAEEDVIRLTSGQNTEDMYYKTITGLKQALPRVQPTSEL----EHDTDPIEQSENLTSLS 500

Query: 481 NSSEKVTESTLEEDEESSNESE---GGLGSLTEMTQAPVDRKTARKENKKKVKEEKREAR 540
           NS EK TES  E DEESS+ESE   G   S    TQ P +RK ARKENKKKVKEEKRE+R
Sbjct: 501 NSCEKETESMSEVDEESSDESEEEDGPSSSTETTTQTPAERKAARKENKKKVKEEKRESR 560

Query: 541 KNKVPKAVKKRKKKLAKSHK 558
           KNKVPKAVKKRKKKLAKSHK
Sbjct: 561 KNKVPKAVKKRKKKLAKSHK 575

BLAST of Lcy13g011650 vs. NCBI nr
Match: XP_038895087.1 (serine/threonine-protein kinase RIO1-like [Benincasa hispida] >XP_038895088.1 serine/threonine-protein kinase RIO1-like [Benincasa hispida])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.7e-291
Identity = 524/557 (94.08%), Postives = 536/557 (96.23%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           MSA+EEKLDEI+PS  NND+  EEDEGEEDLSWSSDSEIGDALDWLDAK D EAVDGAFS
Sbjct: 1   MSAVEEKLDEIEPSAMNNDDSVEEDEGEEDLSWSSDSEIGDALDWLDAKGDREAVDGAFS 60

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSR NSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR
Sbjct: 61  LNARRPNAHGGLYSRQNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFA+K+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 181 ATKSDGQEFAIKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ
Sbjct: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDE +DNYLEEMQEKI ARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 361 TIRELFDFIVDPCLTDEMVDNYLEEMQEKIMARGDISVEDEIADSVFVQSYIPKTLDSVK 420

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSG+DTEDMYYKTITGLKQ+LPRV+P  E+KDEEEH+ADPIE  KN+T LS
Sbjct: 421 HAEEDVIRLTSGRDTEDMYYKTITGLKQALPRVQPAPEIKDEEEHHADPIEPSKNITSLS 480

Query: 481 NSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNK 540
           NS EK TEST EEDEESSNESEG LGS TE TQ PVDRKTARKENKKKVKEEKREARKNK
Sbjct: 481 NSCEKETESTSEEDEESSNESEGELGSSTETTQMPVDRKTARKENKKKVKEEKREARKNK 540

Query: 541 VPKAVKKRKKKLAKSHK 558
           VPKAVKKRKKKLAKSHK
Sbjct: 541 VPKAVKKRKKKLAKSHK 557

BLAST of Lcy13g011650 vs. NCBI nr
Match: XP_008437198.1 (PREDICTED: serine/threonine-protein kinase RIO1-like [Cucumis melo] >TYJ99080.1 serine/threonine-protein kinase RIO1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.6e-289
Identity = 521/557 (93.54%), Postives = 533/557 (95.69%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           M A+EEK+DEI+PST NNDE  EEDEGEEDLSWSSDSEIGDALDWLDAKED E VDGAFS
Sbjct: 6   MPAVEEKVDEIEPSTMNNDESVEEDEGEEDLSWSSDSEIGDALDWLDAKEDRETVDGAFS 65

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR
Sbjct: 66  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 125

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 126 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 185

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFA+K+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 186 ATKSDGQEFAIKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 245

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLR+HVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ
Sbjct: 246 LKAAGIRCPTPLLLRMHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 305

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 306 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 365

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDETIDNYLEE+QEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 366 TIRELFDFIVDPCLTDETIDNYLEEIQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 425

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQDTEDMYYKTITGLKQ+LP  +P S+ KDEEEHN D I   +N T LS
Sbjct: 426 HAEEDVIRLTSGQDTEDMYYKTITGLKQALPIAQPTSDQKDEEEHNTDMIGPSENTTSLS 485

Query: 481 NSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNK 540
           NS EK TEST EEDEESSNESEG LGS TE TQ PVDRKTARKENKKKVKEEKREARKNK
Sbjct: 486 NSCEKETESTSEEDEESSNESEGELGSSTETTQTPVDRKTARKENKKKVKEEKREARKNK 545

Query: 541 VPKAVKKRKKKLAKSHK 558
           +PKAVKKRKKKLAKSHK
Sbjct: 546 IPKAVKKRKKKLAKSHK 562

BLAST of Lcy13g011650 vs. NCBI nr
Match: XP_004137539.1 (serine/threonine-protein kinase RIO1 [Cucumis sativus])

HSP 1 Score: 1003.8 bits (2594), Expect = 5.7e-289
Identity = 518/557 (93.00%), Postives = 534/557 (95.87%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           M A+EEK+DE++PS  NNDE  EEDEGEEDLSWSSDSEIGDALDWLDAKED E VDG FS
Sbjct: 6   MPAVEEKVDEVEPSIMNNDESVEEDEGEEDLSWSSDSEIGDALDWLDAKEDRETVDGTFS 65

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR
Sbjct: 66  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 125

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 126 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 185

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFA+K+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 186 ATKSDGQEFAIKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 245

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLR+HVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ
Sbjct: 246 LKAAGIRCPTPLLLRMHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 305

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQ+VDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 306 KCKLVHGDLSEYNILYFEGHLYIIDVSQSVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 365

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDET+DNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 366 TIRELFDFIVDPCLTDETMDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 425

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQDTEDMYYKTITGLKQ+LP  +P S+LKDEEEHNAD I+  +++TGLS
Sbjct: 426 HAEEDVIRLTSGQDTEDMYYKTITGLKQALPMAQPTSDLKDEEEHNADMIKPSESITGLS 485

Query: 481 NSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNK 540
           NS EK TEST EEDEESSNESEG  GS TE TQ PVDRK ARKENKKKVKEEKREARKNK
Sbjct: 486 NSCEKATESTSEEDEESSNESEGEPGSSTETTQTPVDRKAARKENKKKVKEEKREARKNK 545

Query: 541 VPKAVKKRKKKLAKSHK 558
           VPKAVKKRKKKLAKSHK
Sbjct: 546 VPKAVKKRKKKLAKSHK 562

BLAST of Lcy13g011650 vs. NCBI nr
Match: XP_023542500.1 (serine/threonine-protein kinase RIO1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 971.5 bits (2510), Expect = 3.2e-279
Identity = 513/560 (91.61%), Postives = 532/560 (95.00%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           MSA+EEKL+EI+PSTRNN+E  EED+ EEDLSWSSDSEIG+ALDWLDAKED EA DGAFS
Sbjct: 1   MSAVEEKLEEIEPSTRNNEEPVEEDDEEEDLSWSSDSEIGEALDWLDAKEDREA-DGAFS 60

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPL EWEGRFNVGMSNSVTTAIR
Sbjct: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLGEWEGRFNVGMSNSVTTAIR 120

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFAVK+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 181 ATKSDGQEFAVKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLE+IITMRTLYQ
Sbjct: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEVIITMRTLYQ 300

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQ+VDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQSVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDETID+YLEE+QEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 361 TIRELFDFIVDPCLTDETIDSYLEELQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQ+TEDMYYKTITGLKQ+LPRV+P SEL    EH+ADPIEQ +NLTGLS
Sbjct: 421 HAEEDVIRLTSGQNTEDMYYKTITGLKQALPRVQPASEL----EHDADPIEQSENLTGLS 480

Query: 481 NSSEKVTESTLEEDEESSNES--EGGLGSLTE-MTQAPVDRKTARKENKKKVKEEKREAR 540
           NS EK TES  E DEESS+ES  E G  S TE  TQ P +RK ARKENKKKVKEEKRE+R
Sbjct: 481 NSCEKETESMSEADEESSDESEEEDGPNSSTETTTQTPAERKAARKENKKKVKEEKRESR 540

Query: 541 KNKVPKAVKKRKKKLAKSHK 558
           KNKVPKAVKKRKKKLAKSHK
Sbjct: 541 KNKVPKAVKKRKKKLAKSHK 555

BLAST of Lcy13g011650 vs. NCBI nr
Match: XP_023542499.1 (serine/threonine-protein kinase RIO1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 971.5 bits (2510), Expect = 3.2e-279
Identity = 513/560 (91.61%), Postives = 532/560 (95.00%), Query Frame = 0

Query: 1   MSALEEKLDEIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFS 60
           MSA+EEKL+EI+PSTRNN+E  EED+ EEDLSWSSDSEIG+ALDWLDAKED EA DGAFS
Sbjct: 21  MSAVEEKLEEIEPSTRNNEEPVEEDDEEEDLSWSSDSEIGEALDWLDAKEDREA-DGAFS 80

Query: 61  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIR 120
           LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPL EWEGRFNVGMSNSVTTAIR
Sbjct: 81  LNARRPNAHGGLYSRHNSSTLQPLSNRNQKFTNHIRASPLGEWEGRFNVGMSNSVTTAIR 140

Query: 121 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 180
           DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH
Sbjct: 141 DSVKEMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYH 200

Query: 181 ATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 240
           ATKSDGQEFAVK+YKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR
Sbjct: 201 ATKSDGQEFAVKIYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMR 260

Query: 241 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQ 300
           LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLE+IITMRTLYQ
Sbjct: 261 LKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEVIITMRTLYQ 320

Query: 301 KCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 360
           KCKLVHGDLSEYNILYFEGHLYIIDVSQ+VDLDHPHALDFLREDCLHVSDFFKKHGVAVM
Sbjct: 321 KCKLVHGDLSEYNILYFEGHLYIIDVSQSVDLDHPHALDFLREDCLHVSDFFKKHGVAVM 380

Query: 361 TIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 420
           TIRELFDFIVDPCLTDETID+YLEE+QEKISARGDISVEDEIADSVFVQSYIPKTLDSVK
Sbjct: 381 TIRELFDFIVDPCLTDETIDSYLEELQEKISARGDISVEDEIADSVFVQSYIPKTLDSVK 440

Query: 421 HAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLS 480
           HAEEDVIRLTSGQ+TEDMYYKTITGLKQ+LPRV+P SEL    EH+ADPIEQ +NLTGLS
Sbjct: 441 HAEEDVIRLTSGQNTEDMYYKTITGLKQALPRVQPASEL----EHDADPIEQSENLTGLS 500

Query: 481 NSSEKVTESTLEEDEESSNES--EGGLGSLTE-MTQAPVDRKTARKENKKKVKEEKREAR 540
           NS EK TES  E DEESS+ES  E G  S TE  TQ P +RK ARKENKKKVKEEKRE+R
Sbjct: 501 NSCEKETESMSEADEESSDESEEEDGPNSSTETTTQTPAERKAARKENKKKVKEEKRESR 560

Query: 541 KNKVPKAVKKRKKKLAKSHK 558
           KNKVPKAVKKRKKKLAKSHK
Sbjct: 561 KNKVPKAVKKRKKKLAKSHK 575

BLAST of Lcy13g011650 vs. TAIR 10
Match: AT2G24990.1 (Serine/threonine-protein kinase Rio1 )

HSP 1 Score: 719.9 bits (1857), Expect = 1.5e-207
Identity = 382/538 (71.00%), Postives = 440/538 (81.78%), Query Frame = 0

Query: 20  ELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFSLNARRPNAHGGLYSRHNSS 79
           E E +D+ ++DLS SSDS+I +ALDWLD K+D E + G FSL+ARRPNAHGG  SR NSS
Sbjct: 21  EEEHDDDDDDDLSLSSDSDIAEALDWLDGKDDDELIGGGFSLHARRPNAHGGHGSRPNSS 80

Query: 80  TLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIRDSVKEMAIGKTKTTEKADR 139
           +LQP+SN+ QK T+H+RASPL  WEGR  VGMSNSVTTAIR S+++  IG+++ T+KADR
Sbjct: 81  SLQPISNKAQKLTSHVRASPLEGWEGRVKVGMSNSVTTAIRGSLRDTEIGRSRNTDKADR 140

Query: 140 ATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKSDGQEFAVKVYKTSVL 199
           ATVEQA+DPRTRMVLF+MLNRGVF+D+NGCISTGKEANVYHATKSDG E A+KVYKTSVL
Sbjct: 141 ATVEQALDPRTRMVLFRMLNRGVFNDVNGCISTGKEANVYHATKSDGSELAIKVYKTSVL 200

Query: 200 VFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMRLKAAGIRCPTPLLLRLHVL 259
           VFKDRDRYVQGDYRFR+GYCRHNPRKMVKTWAEKE RNL RL AAGIRCP  +LLRLHVL
Sbjct: 201 VFKDRDRYVQGDYRFRYGYCRHNPRKMVKTWAEKEQRNLKRLHAAGIRCPAVILLRLHVL 260

Query: 260 VMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQKCKLVHGDLSEYNILYFEG 319
           VMEFIG+ GWAAPRLKDAALSL++LRE YLE+II MR LYQKCKLVHGDLSEYNILYFEG
Sbjct: 261 VMEFIGRDGWAAPRLKDAALSLDKLRECYLELIIQMRVLYQKCKLVHGDLSEYNILYFEG 320

Query: 320 HLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVMTIRELFDFIVDPCLTDETI 379
           HLYIIDVSQ+VDLDHP AL+FLREDC HVSDFFKKHGVAVMTIRELFDFIVDP +TDE +
Sbjct: 321 HLYIIDVSQSVDLDHPLALNFLREDCDHVSDFFKKHGVAVMTIRELFDFIVDPAITDENV 380

Query: 380 DNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVKHAEEDVIRLTSGQDTEDMY 439
           D+YLEE+Q K+  RG+ISVEDEIADSVF++SYIPK+LD+V + E DV ++TSGQDT DM 
Sbjct: 381 DSYLEEVQRKVIERGEISVEDEIADSVFMKSYIPKSLDAVNNPEADVAKITSGQDTGDML 440

Query: 440 YKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLSNSSEKVTESTLEEDEESSN 499
           Y+TITGLK +LP++E   E K E                  N  EK  E   EED ES  
Sbjct: 441 YQTITGLKDALPKIE---EQKIE-----------------VNDEEKEEEGEEEEDGES-- 500

Query: 500 ESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNKVPKAVKKRKKKLAKSHK 558
             EG     +E      D+K ARKE+KKKVKEEKRE+RK K PK+VKKRKKK++K HK
Sbjct: 501 -EEGSEEEESEEELGHEDKKAARKEHKKKVKEEKRESRKTKTPKSVKKRKKKVSKPHK 535

BLAST of Lcy13g011650 vs. TAIR 10
Match: AT5G37350.1 (Serine/threonine-protein kinase Rio1 )

HSP 1 Score: 718.8 bits (1854), Expect = 3.4e-207
Identity = 383/548 (69.89%), Postives = 444/548 (81.02%), Query Frame = 0

Query: 10  EIQPSTRNNDELEEEDEGEEDLSWSSDSEIGDALDWLDAKEDTEAVDGAFSLNARRPNAH 69
           +I P     +ELE++D   +DLS SSDS+I +ALDWLD K+D E + G FSL+ARRPNAH
Sbjct: 13  KITPFVEYEEELEDDD---DDLSLSSDSDIAEALDWLDGKDDDELIGGGFSLHARRPNAH 72

Query: 70  GGLYSRHNSSTLQPLSNRNQKFTNHIRASPLAEWEGRFNVGMSNSVTTAIRDSVKEMAIG 129
           GG  SR NSS LQP+SN+ QK T+H+RASPL  WEGR  VGMSNSVTTAIR S+++  IG
Sbjct: 73  GGHGSRPNSSALQPISNKAQKLTSHVRASPLEGWEGRVKVGMSNSVTTAIRGSLRDTEIG 132

Query: 130 KTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKSDGQEF 189
           +++ T+KADRATVEQA+DPRTRMVLF+MLNRGVF+D+NGCISTGKEANVYHATKSDG E 
Sbjct: 133 RSRNTDKADRATVEQALDPRTRMVLFRMLNRGVFNDVNGCISTGKEANVYHATKSDGSEL 192

Query: 190 AVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKEMRNLMRLKAAGIRCP 249
           A+KVYKTSVLVFKDRDRYVQGDYRFR+GYCRHNPRKMVKTWAEKE RNL RL AAGIRCP
Sbjct: 193 AIKVYKTSVLVFKDRDRYVQGDYRFRYGYCRHNPRKMVKTWAEKEQRNLKRLHAAGIRCP 252

Query: 250 TPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIITMRTLYQKCKLVHGDL 309
             +LLRLHVLVMEFIG+ GWAAPRLKDAALSL++LRE YLE+II MR LYQKCKLVHGDL
Sbjct: 253 AVILLRLHVLVMEFIGRDGWAAPRLKDAALSLDKLRECYLELIIQMRVLYQKCKLVHGDL 312

Query: 310 SEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKKHGVAVMTIRELFDFI 369
           SEYNILYFEGHLYIIDVSQ+VDLDHP AL+FLREDC HVSDFFKKHGVAVMTIRELFDFI
Sbjct: 313 SEYNILYFEGHLYIIDVSQSVDLDHPLALNFLREDCDHVSDFFKKHGVAVMTIRELFDFI 372

Query: 370 VDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPKTLDSVKHAEEDVIRL 429
           VDP ++DE +D+YLEE+Q K+  RG+ISVEDEIADSVF++SYIPK+LD+V + E DV ++
Sbjct: 373 VDPTISDENVDSYLEEVQRKVIERGEISVEDEIADSVFMKSYIPKSLDAVNNPEADVAKI 432

Query: 430 TSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFKNLTGLSNSSEKVTES 489
           TSGQDT DM Y+TITGLK +LP+V       DE++                N+ E+  E 
Sbjct: 433 TSGQDTGDMLYQTITGLKDALPKV-------DEQQIEV-------------NAEEEEEEE 492

Query: 490 TLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKREARKNKVPKAVKKRK 549
                EES  ESE  LG        P D+K ARKE+KKKVKEEKRE+RK K PK+VKKRK
Sbjct: 493 EEGSGEESEEESEKELG--------PEDKKAARKEHKKKVKEEKRESRKTKTPKSVKKRK 529

Query: 550 KKLAKSHK 558
           KK++K HK
Sbjct: 553 KKVSKPHK 529

BLAST of Lcy13g011650 vs. TAIR 10
Match: AT5G37350.2 (Serine/threonine-protein kinase Rio1 )

HSP 1 Score: 508.1 bits (1307), Expect = 9.2e-144
Identity = 272/383 (71.02%), Postives = 309/383 (80.68%), Query Frame = 0

Query: 175 EANVYHATKSDGQEFAVKVYKTSVLVFKDRDRYVQGDYRFRHGYCRHNPRKMVKTWAEKE 234
           +ANVYHATKSDG E A+KVYKTSVLVFKDRDRYVQGDYRFR+GYCRHNPRKMVKTWAEKE
Sbjct: 29  QANVYHATKSDGSELAIKVYKTSVLVFKDRDRYVQGDYRFRYGYCRHNPRKMVKTWAEKE 88

Query: 235 MRNLMRLKAAGIRCPTPLLLRLHVLVMEFIGKTGWAAPRLKDAALSLNRLRESYLEIIIT 294
            RNL RL AAGIRCP  +LLRLHVLVMEFIG+ GWAAPRLKDAALSL++LRE YLE+II 
Sbjct: 89  QRNLKRLHAAGIRCPAVILLRLHVLVMEFIGRDGWAAPRLKDAALSLDKLRECYLELIIQ 148

Query: 295 MRTLYQKCKLVHGDLSEYNILYFEGHLYIIDVSQAVDLDHPHALDFLREDCLHVSDFFKK 354
           MR LYQKCKLVHGDLSEYNILYFEGHLYIIDVSQ+VDLDHP AL+FLREDC HVSDFFKK
Sbjct: 149 MRVLYQKCKLVHGDLSEYNILYFEGHLYIIDVSQSVDLDHPLALNFLREDCDHVSDFFKK 208

Query: 355 HGVAVMTIRELFDFIVDPCLTDETIDNYLEEMQEKISARGDISVEDEIADSVFVQSYIPK 414
           HGVAVMTIRELFDFIVDP ++DE +D+YLEE+Q K+  RG+ISVEDEIADSVF++SYIPK
Sbjct: 209 HGVAVMTIRELFDFIVDPTISDENVDSYLEEVQRKVIERGEISVEDEIADSVFMKSYIPK 268

Query: 415 TLDSVKHAEEDVIRLTSGQDTEDMYYKTITGLKQSLPRVEPVSELKDEEEHNADPIEQFK 474
           +LD+V + E DV ++TSGQDT DM Y+TITGLK +LP+V       DE++          
Sbjct: 269 SLDAVNNPEADVAKITSGQDTGDMLYQTITGLKDALPKV-------DEQQIEV------- 328

Query: 475 NLTGLSNSSEKVTESTLEEDEESSNESEGGLGSLTEMTQAPVDRKTARKENKKKVKEEKR 534
                 N+ E+  E      EES  ESE  LG        P D+K ARKE+KKKVKEEKR
Sbjct: 329 ------NAEEEEEEEEEGSGEESEEESEKELG--------PEDKKAARKEHKKKVKEEKR 383

Query: 535 EARKNKVPKAVKKRKKKLAKSHK 558
           E+RK K PK+VKKRKKK++K HK
Sbjct: 389 ESRKTKTPKSVKKRKKKVSKPHK 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54VD86.3e-11343.93Serine/threonine-protein kinase rio1 OS=Dictyostelium discoideum OX=44689 GN=rio... [more]
Q9BRS21.0e-11044.17Serine/threonine-protein kinase RIO1 OS=Homo sapiens OX=9606 GN=RIOK1 PE=1 SV=2[more]
Q922Q21.6e-10843.48Serine/threonine-protein kinase RIO1 OS=Mus musculus OX=10090 GN=Riok1 PE=1 SV=2[more]
O449591.2e-9045.11Serine/threonine-protein kinase RIO1 OS=Caenorhabditis elegans OX=6239 GN=riok-1... [more]
O426501.9e-8538.70Serine/threonine-protein kinase rio1 OS=Schizosaccharomyces pombe (strain 972 / ... [more]
Match NameE-valueIdentityDescription
A0A5D3BH211.2e-28993.54Serine/threonine-protein kinase RIO1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3ATK01.2e-28993.54Serine/threonine-protein kinase RIO1 OS=Cucumis melo OX=3656 GN=LOC103482696 PE=... [more]
A0A0A0LQN22.8e-28993.00Serine/threonine-protein kinase RIO1 OS=Cucumis sativus OX=3659 GN=Csa_1G043280 ... [more]
A0A6J1JYS02.9e-27890.89Serine/threonine-protein kinase RIO1 OS=Cucurbita maxima OX=3661 GN=LOC111490077... [more]
A0A6J1K0V12.9e-27890.89Serine/threonine-protein kinase RIO1 OS=Cucurbita maxima OX=3661 GN=LOC111490077... [more]
Match NameE-valueIdentityDescription
XP_038895087.14.7e-29194.08serine/threonine-protein kinase RIO1-like [Benincasa hispida] >XP_038895088.1 se... [more]
XP_008437198.12.6e-28993.54PREDICTED: serine/threonine-protein kinase RIO1-like [Cucumis melo] >TYJ99080.1 ... [more]
XP_004137539.15.7e-28993.00serine/threonine-protein kinase RIO1 [Cucumis sativus][more]
XP_023542500.13.2e-27991.61serine/threonine-protein kinase RIO1-like isoform X2 [Cucurbita pepo subsp. pepo... [more]
XP_023542499.13.2e-27991.61serine/threonine-protein kinase RIO1-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
AT2G24990.11.5e-20771.00Serine/threonine-protein kinase Rio1 [more]
AT5G37350.13.4e-20769.89Serine/threonine-protein kinase Rio1 [more]
AT5G37350.29.2e-14471.02Serine/threonine-protein kinase Rio1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 519..539
NoneNo IPR availableCOILSCoilCoilcoord: 4..24
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 163..265
e-value: 6.5E-40
score: 137.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 271..412
e-value: 9.9E-47
score: 160.6
NoneNo IPR availablePFAMPF01163RIO1coord: 177..364
e-value: 8.8E-76
score: 253.7
NoneNo IPR availablePIRSRPIRSR038147-3PIRSR038147-3coord: 15..557
e-value: 3.2E-226
score: 750.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 540..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 475..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 516..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..35
NoneNo IPR availablePANTHERPTHR45723:SF2SERINE/THREONINE-PROTEIN KINASE RIO1coord: 17..555
NoneNo IPR availablePANTHERPTHR45723SERINE/THREONINE-PROTEIN KINASE RIO1coord: 17..555
NoneNo IPR availableCDDcd05147RIO1_eukcoord: 166..355
e-value: 4.8544E-147
score: 418.898
IPR000687RIO kinaseSMARTSM00090rio_2coord: 135..371
e-value: 6.6E-125
score: 430.9
IPR017407Serine/threonine-protein kinase Rio1PIRSFPIRSF038147STPK_RIO1coord: 2..557
e-value: 2.3E-221
score: 734.4
IPR018935RIO kinase, conserved sitePROSITEPS01245RIO1coord: 304..315
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 166..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g011650.1Lcy13g011650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0042254 ribosome biogenesis
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity