Lcy13g010940 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g010940
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr13: 12044318 .. 12046214 (-)
RNA-Seq ExpressionLcy13g010940
SyntenyLcy13g010940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCCCCCCGGAGGAGGGATGGTATAAGGCGAACGTGGATGCTGCATATTGTGGGGACCTGTCTCGGGCGGGTGTTGGAGTTGTTGTTCGGGATGAGGTAGGGCGAGTCATGCTGTCAGCTGCTCTTAGCCATGATCATGTGGGGAACTCAGATTTGGCAGAGGGTCTGGCGGTGCTGGACGGAATGAGACTTGTGGTGGAGATGTGATGCACGAATTGCTAACCGCAAGTGTACGAGTCAAGTTATAATATAGTGAAAATAATTTCGAATGTCGTCATCTGAATTGGGTTTCTAGCCAACTTAATTACCGAAAATACTTTTGACCAATTTTATTTAGGGAACACACTTTAATGACAAAGAATGTAAAACTAAACACAAAACTACAGGAATGTGACAATCTAATGATGACAAAGGTCTTAGGGAATTGATTTCACCAATTGGGATTATGGATTGATTCATGGATTATTAACATGCTATGACTCATAAATCTTGGAATACTAAACTCACACATAACTCTCCCGAGCATACATGATTCTCTCACATGCAATGAACCCGATATCTCTATCTAAATTCATTAAATATGCAAGGCATTAAGGTTATCCTTTTTAACGATTTCTAGAATTAACTATTATCTCCCGATCTCTAGCTAAATCCTAGTTATGCACAATGATTTCTAAGCTAAATCCTCTCTCCCGAGTTAGATTTAGTCACAAACATCATTCAATTCATGGCCGGTAAATTGAAAGCACTAAAAACACAATATGCATAAATGTCATCAATAGCAAAAATCCAATAAATCAATCCATAAAGTGCCCAACGACTACATCAATCCCTAAGATTCGAAACTTAGCTACTCATACAAAGCGTTACGATATAGCAAAAGTCTAAAATAGCCATTAAAACTACTAAAGTAAGGAAAAAGAGGAATAAACGCTAAGTAGGCCGAGACCGCACGTCCTCGTCACGATCTCCACGAGCAAAGCCTTCAATCCCAATTTTCTCCACTTGGTTCAGCTTCCACAGCAGCCAATTCTATGAGTTTTCGGCCACAATTGTCCCAAAAGATGTCCTCCAAATTGACCTAATCGCAAAATATATATAGGCTGAAAAATCGACACAGCGTCGCAATGATGTAGCGGCAGCGTCGCGACGCTGTCGCGATACTATGGGATTCTAGAATAGAAACCTCACAGCGTCGAGATGCTGTGACCTGTTGCCGCCTAATCAGAAACGGAAAGTTGGAGCGTCGAGACGCCCTATGGCAAGGGTCTCGACGCTGGTCCTCGGCAAGGCCTCAATGGTGTGCTTCCAACTTCGATTCCCTTTGATTCTTTAGCCTCTTTTCATGTCCCGAACACACGATTAGTTCCAATACTTATTTCCTACAAATACAACATGAAAATGCATAAATTTGGCCATTTAAATATAAAAGACTAGCTAAATTAATTAGGGAAAACTAGCTAGCTATGCTCGCTATCAAGATGGGTTTAGCTCCGGTAATCCTTGAGACTGAATCTATGCAGGTTTATTCGCTGCTGCAAGATTCTGCGATGGTGGATCTGTCTGAGTTCGGTGTGCTGATTTCTGAGGCTCGAAAGGAGGTGTCTGCGCATATTCAGCTCAGATTCAGCTTTACAAGAAGGGAAGGAAACCGTGTTGCCCATGAGTTAGCCCGCTTTGCTTTGAGAGAGAGAGACTGTGAGTATTGGATGGAATCGGTACCTGCGTGTGCCTATCTTCTAACTGAGGAAGATATTTTATCCCTATAACTATTTTTCTTTTTCTTGTGTGCTAATGTCAGGCAGGTGGCGGTTGGAGTTTTGTCAAAGATTCCATGATTTATCCTCAAGCTAGTGAAGATCGAGAATGGAAAGCGAACCAAATAACCATCTAA

mRNA sequence

ATGGTCCCCCCGGAGGAGGGATGGTATAAGGCGAACGTGGATGCTGCATATTGTGGGGACCTGTCTCGGGCGGGTGTTGGAGTTGTTGTTCGGGATGAGGTAGGGCGAGTCATGCTGTCAGCTGCTCTTAGCCATGATCATGTGGGGAACTCAGATTTGGCAGAGGCTCCGGTAATCCTTGAGACTGAATCTATGCAGGTTTATTCGCTGCTGCAAGATTCTGCGATGGTGGATCTGTCTGAGTTCGGTGTGCTGATTTCTGAGGCTCGAAAGGAGGTGTCTGCGCATATTCAGCTCAGATTCAGCTTTACAAGAAGGGAAGGAAACCGTGTTGCCCATGAGTTAGCCCGCTTTGCTTTGAGAGAGAGAGACTGTGGCGGTTGGAGTTTTGTCAAAGATTCCATGATTTATCCTCAAGCTAGTGAAGATCGAGAATGGAAAGCGAACCAAATAACCATCTAA

Coding sequence (CDS)

ATGGTCCCCCCGGAGGAGGGATGGTATAAGGCGAACGTGGATGCTGCATATTGTGGGGACCTGTCTCGGGCGGGTGTTGGAGTTGTTGTTCGGGATGAGGTAGGGCGAGTCATGCTGTCAGCTGCTCTTAGCCATGATCATGTGGGGAACTCAGATTTGGCAGAGGCTCCGGTAATCCTTGAGACTGAATCTATGCAGGTTTATTCGCTGCTGCAAGATTCTGCGATGGTGGATCTGTCTGAGTTCGGTGTGCTGATTTCTGAGGCTCGAAAGGAGGTGTCTGCGCATATTCAGCTCAGATTCAGCTTTACAAGAAGGGAAGGAAACCGTGTTGCCCATGAGTTAGCCCGCTTTGCTTTGAGAGAGAGAGACTGTGGCGGTTGGAGTTTTGTCAAAGATTCCATGATTTATCCTCAAGCTAGTGAAGATCGAGAATGGAAAGCGAACCAAATAACCATCTAA

Protein sequence

MVPPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEAPVILETESMQVYSLLQDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTRREGNRVAHELARFALRERDCGGWSFVKDSMIYPQASEDREWKANQITI
Homology
BLAST of Lcy13g010940 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.7e-13
Identity = 49/143 (34.27%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 2   VPPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEA----- 61
           +PP++  YK N DA++      AG+G+++R++ G+VM SA    +++ + D+AEA     
Sbjct: 35  LPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAVE 94

Query: 62  -----------PVILETESMQVYSLLQDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTR 121
                      PVILET+S ++++L    +  DLSE G ++ +A+   +  +   F+F +
Sbjct: 95  GLQLASKIGVNPVILETDSSRIFNLFSQPS-EDLSETGEIVLKAKNFWTQSLHASFNFVK 154

Query: 122 REGNRVAHELARFALRERDCGGW 129
           REGN+ AH LAR AL  R+   W
Sbjct: 155 REGNKAAHMLARRALLLREFSIW 176

BLAST of Lcy13g010940 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-11
Identity = 45/127 (35.43%), Postives = 75/127 (59.06%), Query Frame = 0

Query: 3    PPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEAPVILET 62
            PP+EG YK N DA++      AG+G+++ ++ G+VM +A    +++ + D+AEA  I   
Sbjct: 993  PPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEA--IAAV 1052

Query: 63   ESMQVYSLL-QDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTRREGNRVAHELARFALR 122
            E +Q+ S +    A+ DLSE G ++ +A+   +  +   F+F +REGN+ AH LAR AL 
Sbjct: 1053 EGLQLASEIGMHPALEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALL 1112

Query: 123  ERDCGGW 129
              +   W
Sbjct: 1113 LHEFSIW 1117

BLAST of Lcy13g010940 vs. ExPASy TrEMBL
Match: A0A2I4DU52 (uncharacterized protein LOC108983489 OS=Juglans regia OX=51240 GN=LOC108983489 PE=4 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 4.4e-11
Identity = 48/142 (33.80%), Postives = 74/142 (52.11%), Query Frame = 0

Query: 3   PPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEAPVILET 62
           PP  GW K NVD+A   +L +A VGVV+RD+VG+V+L+A+     V  ++  E+  I   
Sbjct: 43  PPPTGWLKLNVDSAVFSELHKAEVGVVLRDDVGKVILAASKVEFEVNTAEAIESLAIFRA 102

Query: 63  ----ESMQVYSLLQDS-----------AMVDLSEFGVLISEARKEVSAHIQLRFSFTRRE 122
                SM +  L+ +S            +V  +    L+SE RK +   ++ +F    R+
Sbjct: 103 LQLCTSMGIQRLIMESDCLIAIQVLNNELVSDAMLVPLLSEIRKLLRVFVECKFQHVYRD 162

Query: 123 GNRVAHELARFALRERDCGGWS 130
           GNRVAH LAR+A    +   W+
Sbjct: 163 GNRVAHSLARYAWNVENISMWN 184

BLAST of Lcy13g010940 vs. ExPASy TrEMBL
Match: A0A6J1CDQ4 (uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010533 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 7.5e-11
Identity = 48/139 (34.53%), Postives = 78/139 (56.12%), Query Frame = 0

Query: 3   PPEEGWYKANVDAAYCGDLSRAGVGV-VVRDEVGRVMLSAALSHDHVGNSDLAEA----- 62
           P EEG +K   DA++      AG+GV ++RD  G+V+ SA    +HV + D AEA     
Sbjct: 92  PAEEGVFKLKTDASFSSIDFNAGLGVIIIRDHRGQVLASATKYLEHVASVDDAEALAAVE 151

Query: 63  -----------PVILETESMQVYSLLQDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTR 122
                      P++LET+S+++Y+L        LS+ G +I   +  ++  +Q+ +SFT+
Sbjct: 152 GLRVAMETGISPILLETDSLRIYNLFARD-KEGLSKTGSIIEYVKTHLATRLQVSYSFTK 211

Query: 123 REGNRVAHELARFALRERD 125
           R GN +AH LAR AL+ ++
Sbjct: 212 RTGNTIAHLLARRALQSQE 229

BLAST of Lcy13g010940 vs. ExPASy TrEMBL
Match: B9FIH0 (RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_19645 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 9.2e-09
Identity = 48/142 (33.80%), Postives = 68/142 (47.89%), Query Frame = 0

Query: 3   PPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAE------- 62
           PP EGW K NVD A+     R G+GVV+RD +GRV+LS+         ++ AE       
Sbjct: 45  PPPEGWAKINVDGAFDQGDGRCGIGVVIRDCLGRVLLSSWRYLRRCSQAEEAELLACSEG 104

Query: 63  ---------APVILETESMQVYSLLQDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTRR 122
                     PVILE++ +   + +    M + S +  L+ E +  V    ++      R
Sbjct: 105 INLAAEWIHLPVILESDCLMATTSIAGKDM-ERSRWTFLLREIKAAVRRLQEVSVHHVNR 164

Query: 123 EGNRVAHELARFALRERDCGGW 129
           E NRVAHELA+ A R   C  W
Sbjct: 165 ECNRVAHELAQLAKRTAHCAVW 185

BLAST of Lcy13g010940 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 85.1 bits (209), Expect = 5.7e-13
Identity = 49/143 (34.27%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 2   VPPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEA----- 61
           +PP++  YK N DA++      AG+G+++R++ G+VM SA    +++ + D+AEA     
Sbjct: 35  LPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAVE 94

Query: 62  -----------PVILETESMQVYSLLQDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTR 121
                      PVILET+S ++++L    +  DLSE G ++ +A+   +  +   F+F +
Sbjct: 95  GLQLASKIGVNPVILETDSSRIFNLFSQPS-EDLSETGEIVLKAKNFWTQSLHASFNFVK 154

Query: 122 REGNRVAHELARFALRERDCGGW 129
           REGN+ AH LAR AL  R+   W
Sbjct: 155 REGNKAAHMLARRALLLREFSIW 176

BLAST of Lcy13g010940 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 79.7 bits (195), Expect = 2.4e-11
Identity = 45/127 (35.43%), Postives = 75/127 (59.06%), Query Frame = 0

Query: 3    PPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEAPVILET 62
            PP+EG YK N DA++      AG+G+++ ++ G+VM +A    +++ + D+AEA  I   
Sbjct: 993  PPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEA--IAAV 1052

Query: 63   ESMQVYSLL-QDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTRREGNRVAHELARFALR 122
            E +Q+ S +    A+ DLSE G ++ +A+   +  +   F+F +REGN+ AH LAR AL 
Sbjct: 1053 EGLQLASEIGMHPALEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALL 1112

Query: 123  ERDCGGW 129
              +   W
Sbjct: 1113 LHEFSIW 1117

BLAST of Lcy13g010940 vs. NCBI nr
Match: XP_018810679.2 (uncharacterized protein LOC108983489 [Juglans regia])

HSP 1 Score: 77.8 bits (190), Expect = 9.1e-11
Identity = 48/142 (33.80%), Postives = 74/142 (52.11%), Query Frame = 0

Query: 3   PPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEAPVILET 62
           PP  GW K NVD+A   +L +A VGVV+RD+VG+V+L+A+     V  ++  E+  I   
Sbjct: 43  PPPTGWLKLNVDSAVFSELHKAEVGVVLRDDVGKVILAASKVEFEVNTAEAIESLAIFRA 102

Query: 63  ----ESMQVYSLLQDS-----------AMVDLSEFGVLISEARKEVSAHIQLRFSFTRRE 122
                SM +  L+ +S            +V  +    L+SE RK +   ++ +F    R+
Sbjct: 103 LQLCTSMGIQRLIMESDCLIAIQVLNNELVSDAMLVPLLSEIRKLLRVFVECKFQHVYRD 162

Query: 123 GNRVAHELARFALRERDCGGWS 130
           GNRVAH LAR+A    +   W+
Sbjct: 163 GNRVAHSLARYAWNVENISMWN 184

BLAST of Lcy13g010940 vs. NCBI nr
Match: XP_022139684.1 (uncharacterized protein LOC111010533 [Momordica charantia])

HSP 1 Score: 77.0 bits (188), Expect = 1.5e-10
Identity = 48/139 (34.53%), Postives = 78/139 (56.12%), Query Frame = 0

Query: 3   PPEEGWYKANVDAAYCGDLSRAGVGV-VVRDEVGRVMLSAALSHDHVGNSDLAEA----- 62
           P EEG +K   DA++      AG+GV ++RD  G+V+ SA    +HV + D AEA     
Sbjct: 92  PAEEGVFKLKTDASFSSIDFNAGLGVIIIRDHRGQVLASATKYLEHVASVDDAEALAAVE 151

Query: 63  -----------PVILETESMQVYSLLQDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTR 122
                      P++LET+S+++Y+L        LS+ G +I   +  ++  +Q+ +SFT+
Sbjct: 152 GLRVAMETGISPILLETDSLRIYNLFARD-KEGLSKTGSIIEYVKTHLATRLQVSYSFTK 211

Query: 123 REGNRVAHELARFALRERD 125
           R GN +AH LAR AL+ ++
Sbjct: 212 RTGNTIAHLLARRALQSQE 229

BLAST of Lcy13g010940 vs. NCBI nr
Match: XP_042962749.1 (uncharacterized protein LOC122297028 [Carya illinoinensis])

HSP 1 Score: 76.3 bits (186), Expect = 2.6e-10
Identity = 51/159 (32.08%), Postives = 79/159 (49.69%), Query Frame = 0

Query: 3    PPEEGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAALSHDHVGNSDLAEAPVIL-- 62
            PP +G  K NVD A  G++ +AG+G+V+RD  G V+++ +     V NS+  EA  +L  
Sbjct: 990  PPPQGSLKMNVDGATFGEIDKAGIGLVLRDHEGNVLMTCSKIEHAVANSEFIEATAVLRG 1049

Query: 63   --------------ETESMQVYSLLQDSAMVDLSEFGVLISEARKEVSAHIQLRFSFTRR 122
                          E++ + + ++L  S+   LS+F  +I E R+  S   ++ F    R
Sbjct: 1050 LQFGLQWGINKLVMESDCLFLINVLNSSSEC-LSDFAYVIDEIRRIRSYFHEISFVHVCR 1109

Query: 123  EGNRVAHELARFALRERDCGGWSFVKDSMIYPQASEDRE 146
            +GN VAH LARFA    D   W     S +   A  DR+
Sbjct: 1110 QGNNVAHGLARFARNVDDVSVWRDGCPSCVSQAAWLDRQ 1147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CIF12.7e-1334.27uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1DAR41.2e-1135.43uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A2I4DU524.4e-1133.80uncharacterized protein LOC108983489 OS=Juglans regia OX=51240 GN=LOC108983489 P... [more]
A0A6J1CDQ47.5e-1134.53uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
B9FIH09.2e-0933.80RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Match NameE-valueIdentityDescription
XP_022140628.15.7e-1334.27uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_022150918.12.4e-1135.43uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_018810679.29.1e-1133.80uncharacterized protein LOC108983489 [Juglans regia][more]
XP_022139684.11.5e-1034.53uncharacterized protein LOC111010533 [Momordica charantia][more]
XP_042962749.12.6e-1032.08uncharacterized protein LOC122297028 [Carya illinoinensis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 57..119
e-value: 6.6E-8
score: 32.4
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 58..117
coord: 4..56
NoneNo IPR availablePANTHERPTHR47074:SF25SUBFAMILY NOT NAMEDcoord: 58..117
NoneNo IPR availablePANTHERPTHR47074:SF25SUBFAMILY NOT NAMEDcoord: 4..56
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 12..117
e-value: 1.25874E-14
score: 64.2576
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 7..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g010940.1Lcy13g010940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity