Lcy13g005380 (gene) Sponge gourd (P93075) v1

BLAST of Lcy13g005380 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 9.6e-194
Identity = 338/552 (61.23%), Postives = 413/552 (74.82%), Query Frame = 0

Query: 20  SDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKSCLKQAIKMSSVIGDYL 79
           S S  PS A CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAEDVDEDLVERVESILSAI 139
            R           E GA++DC ELS+ +V +L ++   L  A+ +   LVE V S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNSLSRRWKKRRQKGGNFPA 199
           VTN QTC+DGLVE++S    A+ +P+ +  +LYS+SLGLV+++L+R  K+ +   G    
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 AARN--REPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGST---GIFINNTVVVSSTGA 259
              +  REPL TLIKGL K  +C+    C    R L +LG T    I ++  V+V    +
Sbjct: 254 GGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 260 DNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKTNIMLIGDGINRTVITG 319
           DNFT+I DAIA APNN+ PEDGYFVIY R+G Y+EY+VVP  K N+ML+GDGIN+T+ITG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 320 NHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEG 379
           NHNVVDGWTTYN S+F V G+ F+A+DVTFRNTAGPEKHQAVA+RN+A+ S+FYRCSFEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 380 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKPLPNQKNAFTAQGRTDP 439
           YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR DP
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 440 NQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYSRTVYMQSYIGDLISPV 499
           NQNTGISI NCTIKAA D   +  +A T      FLGRPWK YSRTV+MQSYI D++ PV
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPKSAMT------FLGRPWKPYSRTVFMQSYISDIVQPV 553

Query: 500 GWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQAFNFTVYNFTMGDTWL 559
           GWLEWNGT GLDT+YYGE+ N+GPGANT+ RV W GY+L+N  +A NFTVYNFTMGDTWL
Sbjct: 554 GWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWL 613

Query: 560 PYTDIPFSGGLL 565
           P TDIPF GGLL
Sbjct: 614 PQTDIPFYGGLL 616

BLAST of Lcy13g005380 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 8.4e-190
Identity = 340/554 (61.37%), Postives = 408/554 (73.65%), Query Frame = 0

Query: 22  SPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKSCLKQAIKMSSVIGDYLDR 81
           SP P    CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAEDVDEDLVERVESILSAIVT 141
                      E  A++DC EL++ +VE+L ++   L AAE +   LV+RV S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNSLSR---RWKKRRQK---GG 201
           N QTC+DGLV+++S    A+  PL +  +LYSVSLGLV+++L+R   R+K  + K   GG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 NFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGST---GIFINNTVVVSST 261
           N P     REPL TLIK L K  +C++   C    R L +LG T    I +   V V   
Sbjct: 260 NKPV----REPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 262 GADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKTNIMLIGDGINRTVI 321
             DNF +I +A+A APN++ PE GYFVIY R G Y+EYVV+   K NIMLIGDGIN+T+I
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 322 TGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSF 381
           +GNH+ +DGWTTYNSSTF V GD FVA+DVTFRNTAGPEKHQAVAVRN+AD STFYRCSF
Sbjct: 380 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 439

Query: 382 EGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKPLPNQKNAFTAQGRT 441
           EGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRT
Sbjct: 440 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 499

Query: 442 DPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYSRTVYMQSYIGDLIS 501
           DPNQ TGISI NCTI AA D       AA   +T  FLGRPWK YSRTVY+QSYI D++ 
Sbjct: 500 DPNQKTGISIINCTIGAAPDL------AADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQ 559

Query: 502 PVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQAFNFTVYNFTMGDT 561
           PVGWLEWNGT GLDT+ YGE++N+GPGA+TS RV W GYSL+N  QA NFTVYNFT+GDT
Sbjct: 560 PVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDT 619

Query: 562 WLPYTDIPFSGGLL 565
           WLP TDIPF GGLL
Sbjct: 620 WLPQTDIPFYGGLL 621

BLAST of Lcy13g005380 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 503.8 bits (1296), Expect = 2.5e-141
Identity = 283/575 (49.22%), Postives = 372/575 (64.70%), Query Frame = 0

Query: 7   IFLLLLSLLLAGASDSPP-PSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKSCL 66
           +FL L +L +A  +  P   S+ +CK+T  PK C+S+    + S  D   YG+FS++  L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFL-RSIEAV--LDAAEDV 126
            Q+ K +  I  YL R  ++ L+Q   GAL DCR L+    ++L  S E V    +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEDLVERVESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLSSAGKLYSVSLGLVTN 186
                + ++++LSA +TN QTC+DG+  + SS   + N ++ PL +  KL+SVSL L T 
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFT- 190

Query: 187 SLSRRW--KKRRQKGGNFPAAARN----REPLNTLIKGL--HKMGSCNQSTICSGRKRLL 246
              + W  KK++Q      A  +N     +P      G    KM    ++   S  +R L
Sbjct: 191 ---KGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 250

Query: 247 TDLGS--TGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYV 306
            D  +    + +++ V V+  G  NFT+I +A+  APN +    GYFVIYV  G Y+E V
Sbjct: 251 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 310

Query: 307 VVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPE 366
           V+ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA+++TFRNTAGPE
Sbjct: 311 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 370

Query: 367 KHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRC 426
           KHQAVA+R+SADLS FY CSFE YQDTLY HSLRQFYRECDIYGTVDFIFGNAA VFQ C
Sbjct: 371 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 430

Query: 427 NLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLG 486
           NLY R+P+ NQ NA TAQGRTDPNQNTGISIHNCTIK A D        ++  T   +LG
Sbjct: 431 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDL------VSSNYTVKTYLG 490

Query: 487 RPWKAYSRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGY 546
           RPWK YSRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV WPGY
Sbjct: 491 RPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGY 550

Query: 547 SLMNSTQAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
            ++NST A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 HVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Lcy13g005380 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.5e-133
Identity = 273/591 (46.19%), Postives = 361/591 (61.08%), Query Frame = 0

Query: 5   PFIFLLLLSLLL---AGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 64
           P   L+ LS  L     +S +   S+ +CK+T  PK C+S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSI 63

Query: 65  KSCLKQAIKMSSVIGDYLDRGGDSRLNQREA-GALSDCRELSDSNVEF-LRSIEAVLDAA 124
           +  L Q+ K    +  Y+ R  ++ L+Q     AL DCR L+   +++ L S E V D +
Sbjct: 64  RKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 125 -----EDVDEDLVERVESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLSSAGKL 184
                + +     + ++++LSA +TN QTC++GL  + S     ++   ++ PL +  KL
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 185 YSVSLGLVTNSLSRRWKKRRQKGGNFPAAARNREPLNTLIKGLH---------KMGSCNQ 244
             VSL L T    + W  +++K   F A A+ R   +T  K            KM    +
Sbjct: 184 LGVSLALFT----KGWVPKKKKRAGF-AWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTK 243

Query: 245 STICSGRKRLLTDLGSTG-------IFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPED 304
           +   S  +R L D  S G       + I++ V VS  G  NFT+I  A+A APNN+    
Sbjct: 244 AVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSA 303

Query: 305 GYFVIYVRQGYYQEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGD 364
           G+F+IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V   
Sbjct: 304 GFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAP 363

Query: 365 GFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYG 424
            FVA+++TFRNTAGPEKHQAVA+R+ AD S FY CSFE YQDTLY HSLRQFYRECD+YG
Sbjct: 364 NFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYG 423

Query: 425 TVDFIFGNAAAVFQRCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEM 484
           TVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQNTG SI NCTIK A D   
Sbjct: 424 TVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-- 483

Query: 485 DDPNAATTMTTTNFLGRPWKAYSRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFEN 544
                ++  T   +LGRPWK YSRTVYMQSYI   + PVGW EWNG   L TLYY E+ N
Sbjct: 484 ----VSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNN 543

Query: 545 YGPGANTSMRVNWPGYSLMNSTQAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
            GPG+NT+ RV WPGY ++NST A NFTV    +   W+  T +P++ GL+
Sbjct: 544 TGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Lcy13g005380 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 472.6 bits (1215), Expect = 6.1e-132
Identity = 262/574 (45.64%), Postives = 345/574 (60.10%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           ++S+P  F   +S  +    ++  PS  +C+    P  CRS+L +    P D Y YG+ S
Sbjct: 14  LSSLPSPF---ISAQIPAIGNATSPS-NICRFAPDPSYCRSVLPN---QPGDIYSYGRLS 73

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           ++  L +A +  S+I   LDR G     +   GAL DC+ L+   +++L S     D+ +
Sbjct: 74  LRRSLSRARRFISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTK 133

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            +     E V + LSA +TN QTC++GL  + S   N LS  L +  KLY VSL L    
Sbjct: 134 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL---- 193

Query: 181 LSRRWKKRRQKG----------GNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRL 240
            S+ W  RRQ+             F      + PL        KM    ++   +  +R 
Sbjct: 194 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPL--------KMTERARAVYNTVTRRK 253

Query: 241 LTDLGSTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVV 300
           L    +  + +++ V V   G  NFT+I  AIA APN +   +GYF+IYV  G Y+EYV 
Sbjct: 254 LLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 313

Query: 301 VPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEK 360
           VPK K  +M+IGDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAGP K
Sbjct: 314 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 373

Query: 361 HQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCN 420
            QAVA+R+  DLS FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA V Q CN
Sbjct: 374 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 433

Query: 421 LYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGR 480
           LY R+P   Q N  TAQGRTDPNQNTG +IH CTI+ A D       A +  T   +LGR
Sbjct: 434 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDL------ATSNYTVKTYLGR 493

Query: 481 PWKAYSRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYS 540
           PWK YSRTV MQ+YI   + P GW  W+G   L TLYY E+ N GPG++T+ RV WPGY 
Sbjct: 494 PWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYH 553

Query: 541 LMNSTQAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           ++N+T A NFTV NF +G+ W+  T +PF GGL+
Sbjct: 554 VINATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Lcy13g005380 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.0e-291
Identity = 502/568 (88.38%), Postives = 530/568 (93.31%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL LLSL LAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           V+ CLKQAIKMSSVIG+YLDRGGDSRLN++E GAL DCRELSDSNVEFL+SI+ VL+AAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE+L+ER+ESILSAIVTNGQTCIDGLVESRSSLGNALSAPL SAG+LYSVSLGLV NS
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQK----GGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGS 240
           LSRRWKKR QK    GGNFPAAA++REPLNTLIKGLH MGSCNQST CSGRKR+LTDLG+
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+FAPNNSMP+DGYFVIYVRQGYY EYVVVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQ+CNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYS 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTI AA +W MD        TTTN+LGRPWK +S
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMD-----LNETTTNYLGRPWKPFS 480

Query: 481 RTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQ 540
           RTVYMQSYIGDLISPVGWLEWNGT GLDTLYYGEFENYGPGANTS+RV+W G+SLMN T+
Sbjct: 481 RTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITE 540

Query: 541 AFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           A NFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 AMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Lcy13g005380 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 502/564 (89.01%), Postives = 530/564 (93.97%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+P+IFLLLLSL LAGASDSPPPSAALCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           VK CLKQAIKMSSVIG YL RGG SRLNQREAGAL DCREL+D NVEFLRSIE+VL+AAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE +VERVESILSAIVTNGQTCIDGLVES+SSLG+ALSAPLS+AG+LYSVSLGLV NS
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQKGGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGSTGIF 240
           LSRRWKKRRQ   +FP    NREPL TLIKGL KMGSCNQSTICSGR+RLLTDLG+TGIF
Sbjct: 181 LSRRWKKRRQ---SFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIF 240

Query: 241 INNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKTNIML 300
           INNTVVVSSTGADNFTSIGDAIAF+PNNSMPEDGYFVIYVR+GYY+EYVVVPKYKTNIML
Sbjct: 241 INNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIML 300

Query: 301 IGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSA 360
           IGDGINRTVITGNH+VVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSA
Sbjct: 301 IGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSA 360

Query: 361 DLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKPLPNQ 420
           DLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQ+CNLYARKPLPNQ
Sbjct: 361 DLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQ 420

Query: 421 KNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYSRTVY 480
           KNAFTAQGRTDPNQ+TGISIHNCTI+AA DW MD     +  T  N+LGRPWK YSRTVY
Sbjct: 421 KNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMD-----SNATIANYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQAFNF 540
           MQSYIGDLISPVGWLEWNGTAGL+TLYYGEFEN+GPGANTSMRVNWPGYSLMN+TQA NF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSGGLL 565
           TVYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 TVYNFTMGDTWLPYTDIPFNGGLV 556

BLAST of Lcy13g005380 vs. ExPASy TrEMBL
Match: A0A6J1HU46 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 5.7e-290
Identity = 508/569 (89.28%), Postives = 532/569 (93.50%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDS---PPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSV  IFLLLLSL  AGA DS   PPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLD 120
           KFSVK+CLKQAIKMSSVIGDYLDRGGDSRLNQREAGAL DCR+LSDSNVEFLRSIE+VL+
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAEDVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLV 180
           AA+ VDE+LVERV+SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+SSAG+LYSVSLGLV
Sbjct: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRRWKKRRQK--GGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLG 240
           TNSLSR WKKRR K  GG+  A ARNREPLNTLIKGLHKMGSCNQST CSGRKRLL DLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 STGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYK 300
           +TGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMP+DGYFVIYVR GYY+EYVVVPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360
           TNIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTVCGD FVAIDVTFRNTAGPEKHQAVA
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARK 420
           +R+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQ+CNLYARK
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAY 480
           PLPNQK A TAQGRTDPNQNTGISIH+CTIKAA DWE D  N+    TTTN+LGRPWK Y
Sbjct: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNS----TTTNYLGRPWKQY 480

Query: 481 SRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNST 540
           SRTVYMQ YIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRV+WPGYSLMN T
Sbjct: 481 SRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNIT 540

Query: 541 QAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           QA NFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 QAMNFTVYNLTMGDTWLPYTDIPFSGGLV 564

BLAST of Lcy13g005380 vs. ExPASy TrEMBL
Match: A0A1S3BFR9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.6e-289
Identity = 500/568 (88.03%), Postives = 528/568 (92.96%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFIFLLLLSL  AGAS SPP S ALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           VK C+KQAIKMS+VIGDYLDRG DSRLN+REAGALSDCRELSD NVEFLRSIE+VL+AAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE+LVERVES LSAIVTNGQTC+DGLVESRSSLG ALSAPL SAG+LYSVSLGLV+N+
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQKG---GNFPAAARNREPLNTLIKGLHKMGSC-NQSTICSGRKRLLTDLGS 240
           +SRRWKKRR+KG   G  P   R+RE LNTLIKGLHKM  C N ST CSGRKRLLTDLGS
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKT 300
           +GI INNTVVVSSTGADNFTSIGDAIAFAPNNSMP+DGYFVIYVR+GYY+EYVVVPK+KT
Sbjct: 241 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA+
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAL 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQ+CNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYS 480
           LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAA DW MD     +  TTTN+LGRPWK YS
Sbjct: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMD-----SNTTTTNYLGRPWKQYS 480

Query: 481 RTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQ 540
           RTVYMQSYIGDLISPVGWLEWNGT GL+TLYYGE+ENYGPGANTS+RVNWPG+SLMN TQ
Sbjct: 481 RTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQ 540

Query: 541 AFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           A NFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 AMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Lcy13g005380 vs. ExPASy TrEMBL
Match: A0A6J1G137 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449722 PE=3 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.8e-289
Identity = 505/570 (88.60%), Postives = 531/570 (93.16%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDS----PPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSV  IFLLLLSL  AGA DS    PPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVL 120
           GKFSVK+CLKQAIKMSSVIGDYLDRG DSRLNQREAGAL DCR+LSDSNVEFLRSIE+VL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 DAAEDVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGL 180
           +AA+ VDE+LVERV+SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+SSAG+LYSVSLGL
Sbjct: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRRWKKRRQK--GGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDL 240
           VTNSLSR WKKRR K  GG+  A ARNREPLNTLIKGLHKMGSCNQST CSGRKRLL DL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GSTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKY 300
           G+TGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMP+DGYFVIYVR GYY+EYV+VPKY
Sbjct: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVIVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAV 360
           KTNIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTVCGD FVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 AVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYAR 420
           A+R+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQ+CNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKA 480
           KPLPNQK A TAQGRTDPNQNTGISIH+CTIKAA DWE D  N+    TTTN+LGRPWK 
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNS----TTTNYLGRPWKQ 480

Query: 481 YSRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNS 540
           YSRTVYMQ YIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTS+RV+WPGYSLMN 
Sbjct: 481 YSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNI 540

Query: 541 TQAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           TQA NFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 TQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 565

BLAST of Lcy13g005380 vs. NCBI nr
Match: XP_038891461.1 (probable pectinesterase/pectinesterase inhibitor 25 [Benincasa hispida])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.5e-292
Identity = 507/569 (89.10%), Postives = 527/569 (92.62%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNSVPFIFL LL L  AGA DSPPPS ALCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLFLLFLGFAGAYDSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           VK CLKQAIKMSSVIG YLDRG DSRLN+REAGALSDC+ELSDSNVEFLRSIE VLDAAE
Sbjct: 61  VKQCLKQAIKMSSVIGHYLDRGRDSRLNRREAGALSDCKELSDSNVEFLRSIEGVLDAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE+ VERVESILSAIVTNGQTCIDGLVES SS GN LSAPLS AG+LYSVSLGLV+NS
Sbjct: 121 GVDEERVERVESILSAIVTNGQTCIDGLVESGSSFGNVLSAPLSIAGELYSVSLGLVSNS 180

Query: 181 LSRRWKKRRQK----GGNFPAAARNREPLNTLIKGLHKMGSC-NQSTICSGRKRLLTDLG 240
           LSRRWKKRRQK    GGNFPA  RNREPLNTLIKGLHKMGSC NQS  CSGRKRLLT+LG
Sbjct: 181 LSRRWKKRRQKGNGDGGNFPAGPRNREPLNTLIKGLHKMGSCNNQSRKCSGRKRLLTNLG 240

Query: 241 STGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYK 300
           STGI INNTVVVSSTG DNFTSIGDAIAFAPNNSMP+DGYFVIYVR+GYY+EYVV+PK+K
Sbjct: 241 STGILINNTVVVSSTGGDNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360
           TN+MLIGDGINRTVITGN NV+DGWTTYNSSTFTVCGDGFVAID+TFRNTAGPEKHQAVA
Sbjct: 301 TNVMLIGDGINRTVITGNRNVIDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARK 420
           VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQ+CNLYARK
Sbjct: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAY 480
           PLPNQKNAFTAQGRTDPNQNTGISIHNCTI AA DW MD      + TTTN+LGRPWK Y
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIMAAPDWVMD------SNTTTNYLGRPWKQY 480

Query: 481 SRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNST 540
           SRTVYMQSYIGDLISPVGWLEWNGT GL+TLYYGEFENYGPGANTS+RVNWPGYSLMN T
Sbjct: 481 SRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLT 540

Query: 541 QAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           QA NFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 QAMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Lcy13g005380 vs. NCBI nr
Match: XP_023549932.1 (probable pectinesterase/pectinesterase inhibitor 25 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.3e-292
Identity = 503/568 (88.56%), Postives = 533/568 (93.84%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFIFLL LSL LAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSIPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           VK C+KQAIKMSSVIG+YL+RGGDSRLN++E GAL DCRELSDSNVEFL+SI+ VL+AAE
Sbjct: 61  VKKCIKQAIKMSSVIGEYLNRGGDSRLNRQEFGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE+LVER+ESILSAIVTNGQTCIDGLVESRSSLGNAL+APL SAGKLYSVSLGLV NS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQK----GGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGS 240
           LSRRWKKR+QK    GGNFPAAA++REPLNTLIKGLH MGSCNQST CSGRKRLLTDLG+
Sbjct: 181 LSRRWKKRKQKGDGEGGNFPAAAQSREPLNTLIKGLHTMGSCNQSTKCSGRKRLLTDLGT 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+FAPNNSMP+DGYFVIYVRQGYY EYV+VPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECD+YGTVDFIFGNAAAVFQ+CNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYS 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTIKAA +W MD  + ATT TT N+LGRPWK +S
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMD--SNATTTTTMNYLGRPWKPFS 480

Query: 481 RTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQ 540
           RTVYMQSYIGDLISPVGWLEWNGT GLDTLYYGEFENYG GANT  RVNW G+SLMN T+
Sbjct: 481 RTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTRSRVNWGGHSLMNITE 540

Query: 541 AFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           A NFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 AMNFTVYNFTMGDTWLPYTDIPFSGGLL 566

BLAST of Lcy13g005380 vs. NCBI nr
Match: XP_022976564.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.1e-291
Identity = 502/568 (88.38%), Postives = 530/568 (93.31%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL LLSL LAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           V+ CLKQAIKMSSVIG+YLDRGGDSRLN++E GAL DCRELSDSNVEFL+SI+ VL+AAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE+L+ER+ESILSAIVTNGQTCIDGLVESRSSLGNALSAPL SAG+LYSVSLGLV NS
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQK----GGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGS 240
           LSRRWKKR QK    GGNFPAAA++REPLNTLIKGLH MGSCNQST CSGRKR+LTDLG+
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+FAPNNSMP+DGYFVIYVRQGYY EYVVVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQ+CNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYS 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTI AA +W MD        TTTN+LGRPWK +S
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMD-----LNETTTNYLGRPWKPFS 480

Query: 481 RTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQ 540
           RTVYMQSYIGDLISPVGWLEWNGT GLDTLYYGEFENYGPGANTS+RV+W G+SLMN T+
Sbjct: 481 RTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITE 540

Query: 541 AFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           A NFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 AMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Lcy13g005380 vs. NCBI nr
Match: XP_022150485.1 (probable pectinesterase/pectinesterase inhibitor 25 [Momordica charantia])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.8e-291
Identity = 502/564 (89.01%), Postives = 530/564 (93.97%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+P+IFLLLLSL LAGASDSPPPSAALCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           VK CLKQAIKMSSVIG YL RGG SRLNQREAGAL DCREL+D NVEFLRSIE+VL+AAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE +VERVESILSAIVTNGQTCIDGLVES+SSLG+ALSAPLS+AG+LYSVSLGLV NS
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQKGGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGSTGIF 240
           LSRRWKKRRQ   +FP    NREPL TLIKGL KMGSCNQSTICSGR+RLLTDLG+TGIF
Sbjct: 181 LSRRWKKRRQ---SFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIF 240

Query: 241 INNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKTNIML 300
           INNTVVVSSTGADNFTSIGDAIAF+PNNSMPEDGYFVIYVR+GYY+EYVVVPKYKTNIML
Sbjct: 241 INNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIML 300

Query: 301 IGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSA 360
           IGDGINRTVITGNH+VVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSA
Sbjct: 301 IGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSA 360

Query: 361 DLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKPLPNQ 420
           DLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQ+CNLYARKPLPNQ
Sbjct: 361 DLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQ 420

Query: 421 KNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYSRTVY 480
           KNAFTAQGRTDPNQ+TGISIHNCTI+AA DW MD     +  T  N+LGRPWK YSRTVY
Sbjct: 421 KNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMD-----SNATIANYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQAFNF 540
           MQSYIGDLISPVGWLEWNGTAGL+TLYYGEFEN+GPGANTSMRVNWPGYSLMN+TQA NF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSGGLL 565
           TVYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 TVYNFTMGDTWLPYTDIPFNGGLV 556

BLAST of Lcy13g005380 vs. NCBI nr
Match: KAG6601245.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.2e-291
Identity = 504/568 (88.73%), Postives = 530/568 (93.31%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNSVPFIFLL LSL LAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           VK CLKQAIKMSSVIG+YLDRGGDSRLN++E GAL DCRELSDSNVEFL+SI+ VL+AAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            VDE+LVER+ESILSAIVTNGQTCIDGLVESRSSLGNAL+APL SAGKLYSVSLGLV NS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQK----GGNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGS 240
           LSRRWKKR+QK    GG FPAAA++REPLNTLIKGLH MGSCNQST CSGRKRLLTDLG+
Sbjct: 181 LSRRWKKRKQKGGGEGGKFPAAAQSREPLNTLIKGLHTMGSCNQSTKCSGRKRLLTDLGT 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+FAPNNSMP+DGYFVIYVRQGYY EYV+VPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECD+YGTVDFIFGNAAAVFQ+CNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYS 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTIKAA +W MD    AT  TT N+LGRPWK +S
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMD--LNATKTTTMNYLGRPWKPFS 480

Query: 481 RTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQ 540
           RTVYMQSYIGDLISPVGWLEWNGT GLDTLYYGEFENYG GANTS RVNW G+SLMN T+
Sbjct: 481 RTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITE 540

Query: 541 AFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           A NFTVYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 AMNFTVYNFTMGDTWLPYTDIPFSRGLL 566

BLAST of Lcy13g005380 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 677.9 bits (1748), Expect = 6.8e-195
Identity = 338/552 (61.23%), Postives = 413/552 (74.82%), Query Frame = 0

Query: 20  SDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKSCLKQAIKMSSVIGDYL 79
           S S  PS A CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAEDVDEDLVERVESILSAI 139
            R           E GA++DC ELS+ +V +L ++   L  A+ +   LVE V S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNSLSRRWKKRRQKGGNFPA 199
           VTN QTC+DGLVE++S    A+ +P+ +  +LYS+SLGLV+++L+R  K+ +   G    
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 AARN--REPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGST---GIFINNTVVVSSTGA 259
              +  REPL TLIKGL K  +C+    C    R L +LG T    I ++  V+V    +
Sbjct: 254 GGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 260 DNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKTNIMLIGDGINRTVITG 319
           DNFT+I DAIA APNN+ PEDGYFVIY R+G Y+EY+VVP  K N+ML+GDGIN+T+ITG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 320 NHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEG 379
           NHNVVDGWTTYN S+F V G+ F+A+DVTFRNTAGPEKHQAVA+RN+A+ S+FYRCSFEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 380 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKPLPNQKNAFTAQGRTDP 439
           YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR DP
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 440 NQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYSRTVYMQSYIGDLISPV 499
           NQNTGISI NCTIKAA D   +  +A T      FLGRPWK YSRTV+MQSYI D++ PV
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPKSAMT------FLGRPWKPYSRTVFMQSYISDIVQPV 553

Query: 500 GWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQAFNFTVYNFTMGDTWL 559
           GWLEWNGT GLDT+YYGE+ N+GPGANT+ RV W GY+L+N  +A NFTVYNFTMGDTWL
Sbjct: 554 GWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWL 613

Query: 560 PYTDIPFSGGLL 565
           P TDIPF GGLL
Sbjct: 614 PQTDIPFYGGLL 616

BLAST of Lcy13g005380 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 664.8 bits (1714), Expect = 6.0e-191
Identity = 340/554 (61.37%), Postives = 408/554 (73.65%), Query Frame = 0

Query: 22  SPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKSCLKQAIKMSSVIGDYLDR 81
           SP P    CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAEDVDEDLVERVESILSAIVT 141
                      E  A++DC EL++ +VE+L ++   L AAE +   LV+RV S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNSLSR---RWKKRRQK---GG 201
           N QTC+DGLV+++S    A+  PL +  +LYSVSLGLV+++L+R   R+K  + K   GG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 NFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRLLTDLGST---GIFINNTVVVSST 261
           N P     REPL TLIK L K  +C++   C    R L +LG T    I +   V V   
Sbjct: 260 NKPV----REPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 262 GADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVVVPKYKTNIMLIGDGINRTVI 321
             DNF +I +A+A APN++ PE GYFVIY R G Y+EYVV+   K NIMLIGDGIN+T+I
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 322 TGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSF 381
           +GNH+ +DGWTTYNSSTF V GD FVA+DVTFRNTAGPEKHQAVAVRN+AD STFYRCSF
Sbjct: 380 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 439

Query: 382 EGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCNLYARKPLPNQKNAFTAQGRT 441
           EGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRT
Sbjct: 440 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 499

Query: 442 DPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGRPWKAYSRTVYMQSYIGDLIS 501
           DPNQ TGISI NCTI AA D       AA   +T  FLGRPWK YSRTVY+QSYI D++ 
Sbjct: 500 DPNQKTGISIINCTIGAAPDL------AADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQ 559

Query: 502 PVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYSLMNSTQAFNFTVYNFTMGDT 561
           PVGWLEWNGT GLDT+ YGE++N+GPGA+TS RV W GYSL+N  QA NFTVYNFT+GDT
Sbjct: 560 PVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDT 619

Query: 562 WLPYTDIPFSGGLL 565
           WLP TDIPF GGLL
Sbjct: 620 WLPQTDIPFYGGLL 621

BLAST of Lcy13g005380 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 503.8 bits (1296), Expect = 1.8e-142
Identity = 283/575 (49.22%), Postives = 372/575 (64.70%), Query Frame = 0

Query: 7   IFLLLLSLLLAGASDSPP-PSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKSCL 66
           +FL L +L +A  +  P   S+ +CK+T  PK C+S+    + S  D   YG+FS++  L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFL-RSIEAV--LDAAEDV 126
            Q+ K +  I  YL R  ++ L+Q   GAL DCR L+    ++L  S E V    +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEDLVERVESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLSSAGKLYSVSLGLVTN 186
                + ++++LSA +TN QTC+DG+  + SS   + N ++ PL +  KL+SVSL L T 
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFT- 190

Query: 187 SLSRRW--KKRRQKGGNFPAAARN----REPLNTLIKGL--HKMGSCNQSTICSGRKRLL 246
              + W  KK++Q      A  +N     +P      G    KM    ++   S  +R L
Sbjct: 191 ---KGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 250

Query: 247 TDLGS--TGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYV 306
            D  +    + +++ V V+  G  NFT+I +A+  APN +    GYFVIYV  G Y+E V
Sbjct: 251 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 310

Query: 307 VVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPE 366
           V+ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA+++TFRNTAGPE
Sbjct: 311 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 370

Query: 367 KHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRC 426
           KHQAVA+R+SADLS FY CSFE YQDTLY HSLRQFYRECDIYGTVDFIFGNAA VFQ C
Sbjct: 371 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 430

Query: 427 NLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLG 486
           NLY R+P+ NQ NA TAQGRTDPNQNTGISIHNCTIK A D        ++  T   +LG
Sbjct: 431 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDL------VSSNYTVKTYLG 490

Query: 487 RPWKAYSRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGY 546
           RPWK YSRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV WPGY
Sbjct: 491 RPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGY 550

Query: 547 SLMNSTQAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
            ++NST A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 HVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Lcy13g005380 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 478.0 bits (1229), Expect = 1.0e-134
Identity = 273/591 (46.19%), Postives = 361/591 (61.08%), Query Frame = 0

Query: 5   PFIFLLLLSLLL---AGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 64
           P   L+ LS  L     +S +   S+ +CK+T  PK C+S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSI 63

Query: 65  KSCLKQAIKMSSVIGDYLDRGGDSRLNQREA-GALSDCRELSDSNVEF-LRSIEAVLDAA 124
           +  L Q+ K    +  Y+ R  ++ L+Q     AL DCR L+   +++ L S E V D +
Sbjct: 64  RKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 125 -----EDVDEDLVERVESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLSSAGKL 184
                + +     + ++++LSA +TN QTC++GL  + S     ++   ++ PL +  KL
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 185 YSVSLGLVTNSLSRRWKKRRQKGGNFPAAARNREPLNTLIKGLH---------KMGSCNQ 244
             VSL L T    + W  +++K   F A A+ R   +T  K            KM    +
Sbjct: 184 LGVSLALFT----KGWVPKKKKRAGF-AWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTK 243

Query: 245 STICSGRKRLLTDLGSTG-------IFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPED 304
           +   S  +R L D  S G       + I++ V VS  G  NFT+I  A+A APNN+    
Sbjct: 244 AVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSA 303

Query: 305 GYFVIYVRQGYYQEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGD 364
           G+F+IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V   
Sbjct: 304 GFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAP 363

Query: 365 GFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYG 424
            FVA+++TFRNTAGPEKHQAVA+R+ AD S FY CSFE YQDTLY HSLRQFYRECD+YG
Sbjct: 364 NFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYG 423

Query: 425 TVDFIFGNAAAVFQRCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEM 484
           TVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQNTG SI NCTIK A D   
Sbjct: 424 TVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-- 483

Query: 485 DDPNAATTMTTTNFLGRPWKAYSRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFEN 544
                ++  T   +LGRPWK YSRTVYMQSYI   + PVGW EWNG   L TLYY E+ N
Sbjct: 484 ----VSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNN 543

Query: 545 YGPGANTSMRVNWPGYSLMNSTQAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
            GPG+NT+ RV WPGY ++NST A NFTV    +   W+  T +P++ GL+
Sbjct: 544 TGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Lcy13g005380 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 472.6 bits (1215), Expect = 4.3e-133
Identity = 262/574 (45.64%), Postives = 345/574 (60.10%), Query Frame = 0

Query: 1   MNSVPFIFLLLLSLLLAGASDSPPPSAALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           ++S+P  F   +S  +    ++  PS  +C+    P  CRS+L +    P D Y YG+ S
Sbjct: 14  LSSLPSPF---ISAQIPAIGNATSPS-NICRFAPDPSYCRSVLPN---QPGDIYSYGRLS 73

Query: 61  VKSCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALSDCRELSDSNVEFLRSIEAVLDAAE 120
           ++  L +A +  S+I   LDR G     +   GAL DC+ L+   +++L S     D+ +
Sbjct: 74  LRRSLSRARRFISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTK 133

Query: 121 DVDEDLVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLSSAGKLYSVSLGLVTNS 180
            +     E V + LSA +TN QTC++GL  + S   N LS  L +  KLY VSL L    
Sbjct: 134 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL---- 193

Query: 181 LSRRWKKRRQKG----------GNFPAAARNREPLNTLIKGLHKMGSCNQSTICSGRKRL 240
            S+ W  RRQ+             F      + PL        KM    ++   +  +R 
Sbjct: 194 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPL--------KMTERARAVYNTVTRRK 253

Query: 241 LTDLGSTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPEDGYFVIYVRQGYYQEYVV 300
           L    +  + +++ V V   G  NFT+I  AIA APN +   +GYF+IYV  G Y+EYV 
Sbjct: 254 LLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 313

Query: 301 VPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEK 360
           VPK K  +M+IGDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAGP K
Sbjct: 314 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 373

Query: 361 HQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQRCN 420
            QAVA+R+  DLS FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA V Q CN
Sbjct: 374 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 433

Query: 421 LYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAATDWEMDDPNAATTMTTTNFLGR 480
           LY R+P   Q N  TAQGRTDPNQNTG +IH CTI+ A D       A +  T   +LGR
Sbjct: 434 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDL------ATSNYTVKTYLGR 493

Query: 481 PWKAYSRTVYMQSYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVNWPGYS 540
           PWK YSRTV MQ+YI   + P GW  W+G   L TLYY E+ N GPG++T+ RV WPGY 
Sbjct: 494 PWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYH 553

Query: 541 LMNSTQAFNFTVYNFTMGDTWLPYTDIPFSGGLL 565
           ++N+T A NFTV NF +G+ W+  T +PF GGL+
Sbjct: 554 VINATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559