Lcy13g004600 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy13g004600
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Homocysteine S-methyltransferase family protein
Location	Chr13: 3966567 .. 3969827 (+)
RNA-Seq Expression	Lcy13g004600
Synteny	Lcy13g004600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGCGGTCATTGATGGAGGTCTCGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGCGCTGTTTGCCTCATTAAAGATCCTGACCTAATCAAGAAGGTCCTCTCTTACCGTCTCTGTTTCCTTTGTTTCGGTGCATCACGTTCTCATCGGTGCTTAATGATCGGATTAATTTTTCAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGTTTGTAATGCGGATGGACTGAGTTTTATTTATACCTTTCTCAACTGCATACATGAAAATTTTTGTACAATGCAGTCTGTGACGGCTTTTGCAGTACCTTTTTCTTTTAAAAGTTGAACTTTGGTTTGTTTAAAACTTGATTCGAATTGGATATTTTAATGCTAATGAACAGAATGCATGAGGATCAAAGTGATAACTCCTCTCTAATTGAAGTTAAAGTTCAAAATGAGCCATTTCTATTGTGTCGTGCGATGATGAACCAAAACTGAACCGGTGTGTGTACACTCAACGAATGTGGAGAATTTAATCAAGTTAAAAGCATCGGATTAAAGTGCTTTCTATGGAGTAACATTTGTGGACATTCTAGGTGTTGTAATTGGTGGGCCAAATTATGCTTGCATTATAATTTGTAACGGACGTTGCTAAAAGCGACATTACATAGTCGACAGTATTTAATATCTGGAAGGGAAATGTTTGTGTGCTTACTAATATATGACCACATTGGTTTAGCTCAGTTGTCCAAAATGCAAGCTATAAATCAAGATGCCTCTTCTAAATATATCACCACCTATCTTTTTATAAGTCTGTCAGAATGCCATGCGACCATCCCAGCAGGACAAAGCCTGCCGAGAAGTTTTCCAATGCCAAATGTTGTAGAATTATGTGATCAGTTCATGAGATTTATTGAGATGCATGTAATTTGGCTTTATTACACAGTTAGTTTGATTTGTTATAGAGGCAGTTTCTAATCGTTAGCTTTCTGTTATTCTTCATTGTATTTAAAGACTGAGGGATTTCATTTAATCTTGTAAGAGTTCTTATAGTTAATAAAAACTCTCTTCCCTCACTCACACTTGTTAGGCTTGTACACCTAATGAATAAAAATAATGTGCTTACTCATATTTAGTTTGTGATGTTATATAATATAGCCATCTTGTAAAAGAGCTTCTTTTTGCTATTGAAAATTTCAATAGATATTTGCTTCTGTATAGCTTAGGATTTGTATGAACCAATATATGCCCTCCTCTTAATGAATTTTCTGTTTTCACAACTTAATATACCATATCAGAATTCTCGATACTTTATTAGAATTTAGACCCGCATAATATTGGAGTGCAAAATGTGACACCATTTTGTGCTTAAAATTTCTTAAGAATTCCAATAATCTTTAGTATGTATTTTGCCATGCTTAAATTTTTTTTATAGAAATTATTCCTTCTGTTAAAAATTATAAACAGAATACCAATAATGTACACCAATGTTCAAGCCAGCTAATTACTCTCTCTTGTCACAAATTTTATTCTATTACAGGCCACCATTCCTGGGTTTTTATCAAAGGGGTTCTCCGTGGAAGAAGCAGAGTTGCTCTTGGAAAAGAGTGTTAAATTGGCAGTGGAAGCTCGGGATAATTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGGTCGGAATACAGGTAACTGATACATATGAAACATTTAAATAAGGAGAGTTTGTAAATATATGACATTCGGAGCTAATCCTATTCCAACCCCCCCTCCCCTAACCAGTGGGCATTATGGGCCAGATGTGAATGTGGATAAGCTGAAGGACTTTCATCGACGCAGATTGCAGATTCTTGTCGATGCAGGCCCAGATTTGCTTGCATTTGAGACTATTCCGAATAAACTTGAAAGCCAGGTTTTTTTTTAATGCCAACTCTTCTTATATCATCTTAACTGTGCTTGAATAATAGACTTACTCTTACTGCCACACTGATGTTCCCACTGTAACACTTGTATTGTTATGCTTTGATCTGAGTCATAAAATATTTTAGGAAACAAGTTATGGTTTATTTTTTAATCTGTTCGTCTGTATTTATGAGTTGTACAAGGGATCAAGGTAATTTGACAACCATCATGGCATCGTCAATGGGGGGCATCCATTGCATGAGTAGGTCTTAATTTATGAACTGCTTAATTGGCTATCTTGTTCGTGATCAAATTTGTTTTAAATCCCATTCAAGTTCTAAAATTGATCCCAATTTAATAGTTATATTTTCAGATATTCTATTTTAATTTTCAAACTAGTATTATAACATTTGATTTTGTTGGTCTTGTCTGCCACAACATACTGTTAAGAAGTGGAAGGAAAATGTTGAATGTTTCTTATTACTGTCACGAGGATATGAAGACACTTGTTTCAATTGTTTTTCCAATTTGCTTACGTTTGTCTTTCCTCTCTGTGATTCTGTTTCCAACCAATCTTACGTTTGTTCCCCCTCCCTTCTGGTCTAATTAAAAACATTAGGCGTGCGTGGAGTTGCTCGAAGAAGAAAACATCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTCAGAATGCCCCATCTGGAGAGAGCTTCGAGGAGTGCCTCCGTGCTATTAATAAAAGCGACAAAGTAAATGCAGTTGGCATAAACTGTACTCCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGGTAGGAACCAAATCTCTTATTGCTGATTTGATTTTGTTTTCTTGTTCACTGGTTTTGACACCAAATTTTTTCCTTCAGTTAACAGACAAGCCGATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGGTGAGTCAACTTATCCTCCCCTCCCCCATTGTGATATTTCCATGGCTTGTGGGCCTACATTGTGATATTTCCATAACTAATTTAGTCTCTGGTTTTTGTAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCTAAATTCATTGGTGGCTGTTGTCGAACGACACCATCTATGATCCGAGCAGTATCAAAGATTTTGAAAGAAAGCTCTCACTAG

mRNA sequence

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGCGGTCATTGATGGAGGTCTCGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGCGCTGTTTGCCTCATTAAAGATCCTGACCTAATCAAGAAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCCACCATTCCTGGGTTTTTATCAAAGGGGTTCTCCGTGGAAGAAGCAGAGTTGCTCTTGGAAAAGAGTGTTAAATTGGCAGTGGAAGCTCGGGATAATTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGGTCGGAATACAGTGGGCATTATGGGCCAGATGTGAATGTGGATAAGCTGAAGGACTTTCATCGACGCAGATTGCAGATTCTTGTCGATGCAGGCCCAGATTTGCTTGCATTTGAGACTATTCCGAATAAACTTGAAAGCCAGGCGTGCGTGGAGTTGCTCGAAGAAGAAAACATCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTCAGAATGCCCCATCTGGAGAGAGCTTCGAGGAGTGCCTCCGTGCTATTAATAAAAGCGACAAAGTAAATGCAGTTGGCATAAACTGTACTCCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGTTAACAGACAAGCCGATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCTAAATTCATTGGTGGCTGTTGTCGAACGACACCATCTATGATCCGAGCAGTATCAAAGATTTTGAAAGAAAGCTCTCACTAG

Coding sequence (CDS)

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGCGGTCATTGATGGAGGTCTCGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGCGCTGTTTGCCTCATTAAAGATCCTGACCTAATCAAGAAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCCACCATTCCTGGGTTTTTATCAAAGGGGTTCTCCGTGGAAGAAGCAGAGTTGCTCTTGGAAAAGAGTGTTAAATTGGCAGTGGAAGCTCGGGATAATTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGGTCGGAATACAGTGGGCATTATGGGCCAGATGTGAATGTGGATAAGCTGAAGGACTTTCATCGACGCAGATTGCAGATTCTTGTCGATGCAGGCCCAGATTTGCTTGCATTTGAGACTATTCCGAATAAACTTGAAAGCCAGGCGTGCGTGGAGTTGCTCGAAGAAGAAAACATCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTCAGAATGCCCCATCTGGAGAGAGCTTCGAGGAGTGCCTCCGTGCTATTAATAAAAGCGACAAAGTAAATGCAGTTGGCATAAACTGTACTCCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGTTAACAGACAAGCCGATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCTAAATTCATTGGTGGCTGTTGTCGAACGACACCATCTATGATCCGAGCAGTATCAAAGATTTTGAAAGAAAGCTCTCACTAG

Protein sequence

MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH

Homology

[Close]

BLAST of Lcy13g004600 vs. ExPASy Swiss-Prot
Match: A4ZGQ8 (Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN=HMT1 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 4.2e-149
Identity = 242/324 (74.69%), Postives = 285/324 (87.96%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MG++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIKDP+LIK+VH+
Sbjct: 1   MGLEKKSALLEDLIEKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKDPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGAD++V+SSYQATIPGFLS+G S+EE+E LL+KSVKLAVEARD FWD V    GH
Sbjct: 61  EYLEAGADVVVTSSYQATIPGFLSRGLSMEESESLLQKSVKLAVEARDRFWDKVSKTSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSG YG DV++DKLKDFHRRR+Q+LV+A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGSYGEDVSLDKLKDFHRRRIQVLVEASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIP+WICF+SVDG+NAPSGESF+ECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPAWICFTSVDGENAPSGESFQECLETLNKSNNICAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           C PP F++ LI KF +LT K IVVYPNSGEVWDG+AKKWLPS CFGD +FE  +++WRDL
Sbjct: 241 CAPPQFMDNLIRKFSKLTQKAIVVYPNSGEVWDGKAKKWLPSQCFGDAEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILK 325
           GAK IGGCCRTTPS I+A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTIKAISRDLK 324

[Close]

BLAST of Lcy13g004600 vs. ExPASy Swiss-Prot
Match: Q9SDL7 (Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 2.7e-148
Identity = 242/324 (74.69%), Postives = 285/324 (87.96%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           C PP FIE LI KF +LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  +++WRDL
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILK 325
           GAK IGGCCRTTPS I A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

[Close]

BLAST of Lcy13g004600 vs. ExPASy Swiss-Prot
Match: Q9FUN0 (Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 3.3e-130
Identity = 218/316 (68.99%), Postives = 255/316 (80.70%), Query Frame = 0


Query: 9   LLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGAD 68
           +L+DL+  AGGCAVIDGG ATQLE  GA  ND LWSA CLI  P L+K+VH++YLEAGAD
Sbjct: 3   VLEDLVARAGGCAVIDGGFATQLEALGADINDPLWSAACLITRPHLVKEVHMQYLEAGAD 62

Query: 69  ILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHNYNRALVA 128
           +++SSSYQATIPGF+++G SV EAE LL  SVKLA EARD FW S        YNRALVA
Sbjct: 63  VIISSSYQATIPGFIARGMSVAEAEDLLRTSVKLANEARDEFWKSTLRKSKPIYNRALVA 122

Query: 129 ASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNKLESQ 188
           ASIGSYGAYLADGSEYSG YG D+  +KLKDFHRRRLQ+L  AGPDL+AFE IPN++E+Q
Sbjct: 123 ASIGSYGAYLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNQMEAQ 182

Query: 189 ACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPPHFIE 248
           A VELLEEE +QIPSWICFSSVDG+N  SGESF +CL+ +N S+KV  VG+NCTPP FIE
Sbjct: 183 ALVELLEEEKVQIPSWICFSSVDGKNLCSGESFADCLKILNASEKVAVVGVNCTPPQFIE 242

Query: 249 ALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGC 308
            +IC+F++ T K I VYPNSGEVWDGRAK+WLP  C G   F++L+ RW++ GA  IGGC
Sbjct: 243 GIICEFRKQTKKAIAVYPNSGEVWDGRAKRWLPVECLGHKSFDALAKRWQEAGASLIGGC 302

Query: 309 CRTTPSMIRAVSKILK 325
           CRTTPS IRAVSKILK
Sbjct: 303 CRTTPSTIRAVSKILK 318

[Close]

BLAST of Lcy13g004600 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-103
Identity = 179/319 (56.11%), Postives = 232/319 (72.73%), Query Frame = 0


Query: 10  LDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGADI 69
           + D L+  GG AVIDGGLAT+ E+HGA  ND LWSA CL+  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHN-----YNR 129
           + S+SYQATI GF +KGFS EE+E LL+KSV++A EAR++++D   +    +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + ++KLKDFHRRRLQ+L ++G DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPP 249
           +E+QA  +LLEE +++IP W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKF 309
            FIE L+ + +++T KPI+VYPNSGE +D   K+W+ +   GD+ F S   +W D G   
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 310 IGGCCRTTPSMIRAVSKIL 324
           +GGCCRTTP+ IRA+ K L
Sbjct: 309 LGGCCRTTPTTIRAIHKRL 327

[Close]

BLAST of Lcy13g004600 vs. ExPASy Swiss-Prot
Match: Q8LAX0 (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 375.9 bits (964), Expect = 4.5e-103
Identity = 182/331 (54.98%), Postives = 238/331 (71.90%), Query Frame = 0


Query: 4   KKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYL 63
           ++ ++L+ D L+  GG AV+DGG AT+L++HGA  ND LWSA CLI  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNF--------WDSVK 123
           E+GA+I++++SYQATI GF++KG SV EAE LL +SV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDF-- 126

Query: 124 SIPGHNYNR-ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPD 183
           +  G    R  LVAAS+GSYGAYLADGSEYSG YG  V+ + LKDFHRRR+QIL  +G D
Sbjct: 127 AYAGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGAD 186

Query: 184 LLAFETIPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKV 243
           L+AFETIPNKLE++A  +LLEEE+I IP+W  F+S DG + P G+S  EC +  +    V
Sbjct: 187 LIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNV 246

Query: 244 NAVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS 303
            A+GINCT P +I ALI   +++T KPIVVYPNSGEV+DG  KKW+ S    ++ F S  
Sbjct: 247 VAIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYV 306

Query: 304 SRWRDLGAKFIGGCCRTTPSMIRAVSKILKE 326
           S+WRD GA   GGCCRTTP+ IRA++K+L +
Sbjct: 307 SKWRDAGASLFGGCCRTTPNTIRAIAKVLSD 335

[Close]

BLAST of Lcy13g004600 vs. ExPASy TrEMBL
Match: A0A6J1G1A5 (homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.6e-180
Identity = 306/328 (93.29%), Postives = 317/328 (96.65%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAG+DILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESSH 329
           GAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Lcy13g004600 vs. ExPASy TrEMBL
Match: A0A6J1HZT4 (homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467714 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 4.8e-180
Identity = 306/328 (93.29%), Postives = 317/328 (96.65%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKA TLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QI SWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESSH 329
           GAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Lcy13g004600 vs. ExPASy TrEMBL
Match: A0A6J1D951 (homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111018744 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.2e-177
Identity = 302/327 (92.35%), Postives = 315/327 (96.33%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M MKKATTLLDDL++NAGGCAVIDGG ATQLEKHGAV ND LWSAVCLIKDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+VSSSYQATIPGFLSKG S EE ELLLEKSVKLAVEARD+FW+SVKSIPGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSG+YGPDVNVDKLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECLRAINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFES SSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESS 328
           GAKFIGGCCRTTPS  RAVSK+LKESS
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKESS 327

[Close]

BLAST of Lcy13g004600 vs. ExPASy TrEMBL
Match: A0A1S3BEY1 (homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.0e-175
Identity = 298/328 (90.85%), Postives = 312/328 (95.12%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MG++KATTLLDDLL NAGGCAVIDGGLATQLEKHGAVFND LWSAVCLI DPDLIKKVHL
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILV+SSYQATIPGF+SKG SVEE ELLLEKSVKLA+EARD+FWDSVK IPGH
Sbjct: 61  EYLEAGADILVTSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRL+ILVDA PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR KKWLPSNCFGDDKFES +SRW  L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESFTSRWCKL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESSH 329
           GA FIGGCCRTTPS IRAVSK+LKESSH
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKESSH 328

[Close]

BLAST of Lcy13g004600 vs. ExPASy TrEMBL
Match: A0A0A0KR42 (Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660 PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.9e-173
Identity = 297/327 (90.83%), Postives = 308/327 (94.19%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MG+KKATTLLDD L NAGGCAVIDGG ATQLEKHGAV ND LWSAVCLI DP LIKKVHL
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGF+SKG SVEE ELLLEKSVKLA+EARD+FWDSVK IPGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQI VDA PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEENIQIPSWICFSSVDG+NAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR KKWLPSNCFGDDKFESLSSRWR+L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESLSSRWRNL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESS 328
           GA FIGGCCRTTPS IRAVSK+LKES+
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKEST 327

[Close]

BLAST of Lcy13g004600 vs. NCBI nr
Match: XP_023541078.1 (homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647.5 bits (1669), Expect = 6.1e-182
Identity = 309/328 (94.21%), Postives = 319/328 (97.26%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESSH 329
           GAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Lcy13g004600 vs. NCBI nr
Match: KAG6573963.1 (Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013023.1 Homocysteine S-methyltransferase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 642.1 bits (1655), Expect = 2.6e-180
Identity = 307/328 (93.60%), Postives = 318/328 (96.95%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PG+
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESSH 329
           GAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Lcy13g004600 vs. NCBI nr
Match: XP_022945567.1 (homocysteine S-methyltransferase 1 [Cucurbita moschata])

HSP 1 Score: 641.7 bits (1654), Expect = 3.4e-180
Identity = 306/328 (93.29%), Postives = 317/328 (96.65%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAG+DILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESSH 329
           GAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Lcy13g004600 vs. NCBI nr
Match: XP_022968494.1 (homocysteine S-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 640.2 bits (1650), Expect = 9.8e-180
Identity = 306/328 (93.29%), Postives = 317/328 (96.65%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKA TLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QI SWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESSH 329
           GAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Lcy13g004600 vs. NCBI nr
Match: XP_022150665.1 (homocysteine S-methyltransferase 1 [Momordica charantia])

HSP 1 Score: 631.3 bits (1627), Expect = 4.6e-177
Identity = 302/327 (92.35%), Postives = 315/327 (96.33%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M MKKATTLLDDL++NAGGCAVIDGG ATQLEKHGAV ND LWSAVCLIKDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+VSSSYQATIPGFLSKG S EE ELLLEKSVKLAVEARD+FW+SVKSIPGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSG+YGPDVNVDKLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECLRAINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFES SSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILKESS 328
           GAKFIGGCCRTTPS  RAVSK+LKESS
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKESS 327

[Close]

BLAST of Lcy13g004600 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 1.9e-149
Identity = 242/324 (74.69%), Postives = 285/324 (87.96%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           C PP FIE LI KF +LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  +++WRDL
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILK 325
           GAK IGGCCRTTPS I A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

[Close]

BLAST of Lcy13g004600 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 3.8e-137
Identity = 228/324 (70.37%), Postives = 270/324 (83.33%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + A    
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA---- 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
                           LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  +++WRDL
Sbjct: 241 ----------------LTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSMIRAVSKILK 325
           GAK IGGCCRTTPS I A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 304

[Close]

BLAST of Lcy13g004600 vs. TAIR 10
Match: AT3G25900.2 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 414.5 bits (1064), Expect = 8.2e-116
Identity = 193/260 (74.23%), Postives = 229/260 (88.08%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDK 261
           C PP FIE LI KF + TD+
Sbjct: 241 CAPPQFIENLIRKFAKATDE 260

[Close]

BLAST of Lcy13g004600 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-104
Identity = 179/319 (56.11%), Postives = 232/319 (72.73%), Query Frame = 0


Query: 10  LDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGADI 69
           + D L+  GG AVIDGGLAT+ E+HGA  ND LWSA CL+  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHN-----YNR 129
           + S+SYQATI GF +KGFS EE+E LL+KSV++A EAR++++D   +    +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + ++KLKDFHRRRLQ+L ++G DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPP 249
           +E+QA  +LLEE +++IP W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKF 309
            FIE L+ + +++T KPI+VYPNSGE +D   K+W+ +   GD+ F S   +W D G   
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 310 IGGCCRTTPSMIRAVSKIL 324
           +GGCCRTTP+ IRA+ K L
Sbjct: 309 LGGCCRTTPTTIRAIHKRL 327

[Close]

BLAST of Lcy13g004600 vs. TAIR 10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3 )

HSP 1 Score: 375.9 bits (964), Expect = 3.2e-104
Identity = 182/331 (54.98%), Postives = 238/331 (71.90%), Query Frame = 0


Query: 4   KKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYL 63
           ++ ++L+ D L+  GG AV+DGG AT+L++HGA  ND LWSA CLI  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNF--------WDSVK 123
           E+GA+I++++SYQATI GF++KG SV EAE LL +SV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDF-- 126

Query: 124 SIPGHNYNR-ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPD 183
           +  G    R  LVAAS+GSYGAYLADGSEYSG YG  V+ + LKDFHRRR+QIL  +G D
Sbjct: 127 AYAGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGAD 186

Query: 184 LLAFETIPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKV 243
           L+AFETIPNKLE++A  +LLEEE+I IP+W  F+S DG + P G+S  EC +  +    V
Sbjct: 187 LIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNV 246

Query: 244 NAVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS 303
            A+GINCT P +I ALI   +++T KPIVVYPNSGEV+DG  KKW+ S    ++ F S  
Sbjct: 247 VAIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYV 306

Query: 304 SRWRDLGAKFIGGCCRTTPSMIRAVSKILKE 326
           S+WRD GA   GGCCRTTP+ IRA++K+L +
Sbjct: 307 SKWRDAGASLFGGCCRTTPNTIRAIAKVLSD 335

The following BLAST results are available for this feature:

BLAST of Lcy13g004600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4ZGQ8	4.2e-149	74.69	Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN...	[more]
Q9SDL7	2.7e-148	74.69	Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1...	[more]
Q9FUN0	3.3e-130	68.99	Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1	[more]
Q9M1W4	1.6e-103	56.11	Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1...	[more]
Q8LAX0	4.5e-103	54.98	Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ...	[more]

BLAST of Lcy13g004600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1A5	1.6e-180	93.29	homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762...	[more]
A0A6J1HZT4	4.8e-180	93.29	homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467...	[more]
A0A6J1D951	2.2e-177	92.35	homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC11101874...	[more]
A0A1S3BEY1	6.0e-175	90.85	homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 ...	[more]
A0A0A0KR42	1.9e-173	90.83	Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660...	[more]

BLAST of Lcy13g004600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023541078.1	6.1e-182	94.21	homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6573963.1	2.6e-180	93.60	Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022945567.1	3.4e-180	93.29	homocysteine S-methyltransferase 1 [Cucurbita moschata]	[more]
XP_022968494.1	9.8e-180	93.29	homocysteine S-methyltransferase 1-like [Cucurbita maxima]	[more]
XP_022150665.1	4.6e-177	92.35	homocysteine S-methyltransferase 1 [Momordica charantia]	[more]

BLAST of Lcy13g004600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25900.1	1.9e-149	74.69	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.3	3.8e-137	70.37	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.2	8.2e-116	74.23	Homocysteine S-methyltransferase family protein	[more]
AT3G63250.1	1.1e-104	56.11	homocysteine methyltransferase 2	[more]
AT3G22740.1	3.2e-104	54.98	homocysteine S-methyltransferase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017226	Betaine-homocysteine S-methyltransferase, BHMT	PIRSF	PIRSF037505	BHMT	coord: 287..327 e-value: 0.12 score: 9.6 coord: 1..259 e-value: 2.2E-8 score: 31.7
IPR036589	Homocysteine-binding domain superfamily	GENE3D	3.20.20.330		coord: 9..321 e-value: 6.3E-99 score: 333.2
IPR036589	Homocysteine-binding domain superfamily	SUPERFAMILY	82282	Homocysteine S-methyltransferase	coord: 17..325
IPR003726	Homocysteine-binding domain	PFAM	PF02574	S-methyl_trans	coord: 22..322 e-value: 7.0E-74 score: 249.1
IPR003726	Homocysteine-binding domain	PROSITE	PS50970	HCY	coord: 9..323 score: 49.300301
None	No IPR available	PIRSR	PIRSR037505-2	PIRSR037505-2	coord: 9..242 e-value: 1.1E-9 score: 35.6
None	No IPR available	PANTHER	PTHR46015	ZGC:172121	coord: 1..326
None	No IPR available	PANTHER	PTHR46015:SF7	HOMOCYSTEINE S-METHYLTRANSFERASE 1	coord: 1..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy13g004600.1	Lcy13g004600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0033528	S-methylmethionine cycle
molecular_function	GO:0047150	betaine-homocysteine S-methyltransferase activity
molecular_function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function	GO:0008270	zinc ion binding