Lcy13g003060 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g003060
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionmethyltransferase-like protein 7A
LocationChr13: 2608277 .. 2611070 (-)
RNA-Seq ExpressionLcy13g003060
SyntenyLcy13g003060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGAAAGTACAGTGAGATTTCAGATTTTTCTTCCTCCCCTTTTTTACAGAAGTGCAATCACACACAGCCTGGGTATCAATAACTGGGCCAAATCAGGCAACAAAAATGATAAACAGCAAAATTCAAGCAACATGATCCTTTACGCCTCTCCACCAAGTTCAATCATCTTCTCCAAACGCAATGCTCCTCAACAAGCCACACCAAGAAGACCATCGCCCTCCAACTGTGCGCACCAGAGCAGCCCTGAAGCGCCGAAGACAGTGCCACCATTCCCACGTTTGTTTTGCCACAACCATTCTTGCCTTTGCGGAAGGAGGCGTTTCATTGAAGCAGCCGCCACATCTCTGCTCCCTCTATGTCCTTCAATGGCTTCTTCTAATTCATCTTCTGATTACACTGTGCGTATTTTCTTTCGAAGCAGACTGATGGGTTTTTCTCCCTTAAATCAATAACCACAAGTTACTTATCGATTTTTCCCCTAAGACCCAGATGATGAAGTTGATTAGTTTTGTTGTATATAGGCTGGACTGAACAGGGTTCATCCTCCTAAGCCAGACTGGTACGAGGATTTCTATGCGTCTGTTCTGGCTAATGGCATGAAATTGTATGAAGCGGAGGTTGGAGCTAAGTTACGTTACAGTTGATTGGAATTTGTGAACTGCTAACATTGTTGCTATGTTTTTGCTATCTTTCTTGTTTGGTCAGATTGCAGATTACAAGTCCCAGATGTTTTCCAATTTGAGAGGGAAGGCCCAGAAAGTCTTGGAGATTGGAATCGGTACAGGGCCTAATCTTAAATATTACGCTGGGTATGAAGGTATGAAGGTTTATGGTGTGGATCCAAATCTAAAGATGGAGAAATATGCTCGGGAAGCAGCAAAGAATGCTGGCCTTCCAGCTGAGAATTTTGAGTTTAAACAAGCGGTATGGCACTTTATAGAACTCTGCTGTTCTTTATTGAATTTCATAGAATTATTTTATAAAACCAGGGCAATATCTTCAGGTCCTTGTCTTTGTTATTGAATGTAAAAATCTTTCTAATTTAGGTTAATTTGACCCATGAAGCATCCCAGTTGGTAGGAATGGGTTTCTATTTCAACATGATTGGTTAGACAAGTCTGATGGATAGGGTCACTTGCACTGTCTTACGAGTAAAGTTTTCTGCCTTTTTACCTCCTTTGTTTGATAAGACATGTTGTGTTTCTGAGATCTTGAAGCCAGTGGATGTTGAACTAAAACTTTCATATTAAACAGTTGCATCTTCATAATTAGAAGTTGGTTAACTTAACAAATGATGTTATCAATTTGTAACTAAGCCAATTTTCCTTTAGTTACTTCAGCTGTTTGTTTTAAACACATGTTAGATGTGCTCTCTAAAAGCATTTAAAATTATCTTTTATATGTTTTTGGGGATCATACATACTTTCTAATGCATTCCACACAAAAATTTGGATAAGCGTTTGAGTTAGTTGAAAAGTACCTCCAAACACACTGTTATTCTAATAGTTTAATGCTCAAACAGAGTTGGTTTTTTATTGGAAGATTGAAGTTCACTCATTATTTGGAAGTTTGGATTGATTAATCTCTGATCTTTGACTAGGTCGGGGAGGCTATACCATTACCCGATGCTTCTGTCGATGCTGTTGTTGGAACACTTGTCTTGTGTTCTGTGACGAATGTTGACATGACTCTTAAGGGTAATACCTTCTTTTCATTGTAGTGTAATTTGGTAGAGAATCTGACTTCCATTAGTTAGATAAGCAACGTTTCCAAAACTCAGAAACTTCATATAGAGACTTTGTGCTGACTTTTTTGAACTTTGGTTAGACCATCCTGAGCAGCTCCACAGTAACCTGTGCATTGACTTTGCTTCTGCATTTTGGCAGGCTATCTATTGCTCGATTGATGATGGCATAACAAAGTATACTTGCTTAAAAATTCAGGCAGGAGATTTAGATGATATCTATATATGAAATAACTTAGCTTGTTATCCTTAGCATTGGCTTAATGACAGAAGAATCCCTTTGAAAGACACAGAAGTCAAAGTCGTTTCCATCACATAGTGATGAAATTTAAGAGTGGGCTTAGGCAATGGAAAACAAAGTCCTGTCCTTTGAATAATGATAGATGAAAACAAATGAAACTATGCTACTTAAACTTGTCTAATTCTCATCCCCGTTTACTTTTACACGGGCAGATATACCAAATATATTTCTCGATGACACTATTATAGGAAGTACTAGAAACTTTCTAGGACATGTTTGTGAGTGACCAAAATCACTTTTGTCATGTCCAAAATAATAAAAAAGAGCGTGACAAAAGTGATTTTGGTTCCAAAATCACTCCCAAACATGTTTTTAATCCATCTATATTTGTCAAACTTGTAACAATATGAATCTCAACAAAAGGTGCATTTCTTTTGATGATGTGTAGAGGTGAAGAGAGTTCTGAGACCCGGCGGACTATACATATTTGTCGAACATGTGGCTGCAAAAGGTATTATAGCGAGTCTCATCAATTCACCAGTTCGATAAAACCATGTGTTTAGACATAACACAACCAATGGAGCATTTTTGTTGTTGTTGTGTCGATGTAGATGGAACCGTGCTTCGATTTATTCAGGACGTGCTCGATCCTCTGCAGCAGATAGTTTCTGATGGATGTCACCTTACCCGGAGGACAGGGCAGAGTATAACAGAAGCAGGGTTTTCAAATGTTGATCTAGACATGGCTTCCTTCTCTAATGCTGCCTTCATAAATCCTCAAGTTTATGGAGTTGCTTATAGATGA

mRNA sequence

ATGTCTGAAAGTACAGTGAGATTTCAGATTTTTCTTCCTCCCCTTTTTTACAGAAGTGCAATCACACACAGCCTGGGTATCAATAACTGGGCCAAATCAGGCAACAAAAATGATAAACAGCAAAATTCAAGCAACATGATCCTTTACGCCTCTCCACCAAGTTCAATCATCTTCTCCAAACGCAATGCTCCTCAACAAGCCACACCAAGAAGACCATCGCCCTCCAACTGTGCGCACCAGAGCAGCCCTGAAGCGCCGAAGACAGTGCCACCATTCCCACGTTTGTTTTGCCACAACCATTCTTGCCTTTGCGGAAGGAGGCGTTTCATTGAAGCAGCCGCCACATCTCTGCTCCCTCTATGTCCTTCAATGGCTTCTTCTAATTCATCTTCTGATTACACTGCTGGACTGAACAGGGTTCATCCTCCTAAGCCAGACTGGTACGAGGATTTCTATGCGTCTGTTCTGGCTAATGGCATGAAATTGTATGAAGCGGAGATTGCAGATTACAAGTCCCAGATGTTTTCCAATTTGAGAGGGAAGGCCCAGAAAGTCTTGGAGATTGGAATCGGTACAGGGCCTAATCTTAAATATTACGCTGGGTATGAAGGTATGAAGGTTTATGGTGTGGATCCAAATCTAAAGATGGAGAAATATGCTCGGGAAGCAGCAAAGAATGCTGGCCTTCCAGCTGAGAATTTTGAGTTTAAACAAGCGGTCGGGGAGGCTATACCATTACCCGATGCTTCTGTCGATGCTGTTGTTGGAACACTTGTCTTGTGTTCTGTGACGAATGTTGACATGACTCTTAAGGAGGTGAAGAGAGTTCTGAGACCCGGCGGACTATACATATTTGTCGAACATGTGGCTGCAAAAGATGGAACCGTGCTTCGATTTATTCAGGACGTGCTCGATCCTCTGCAGCAGATAGTTTCTGATGGATGTCACCTTACCCGGAGGACAGGGCAGAGTATAACAGAAGCAGGGTTTTCAAATGTTGATCTAGACATGGCTTCCTTCTCTAATGCTGCCTTCATAAATCCTCAAGTTTATGGAGTTGCTTATAGATGA

Coding sequence (CDS)

ATGTCTGAAAGTACAGTGAGATTTCAGATTTTTCTTCCTCCCCTTTTTTACAGAAGTGCAATCACACACAGCCTGGGTATCAATAACTGGGCCAAATCAGGCAACAAAAATGATAAACAGCAAAATTCAAGCAACATGATCCTTTACGCCTCTCCACCAAGTTCAATCATCTTCTCCAAACGCAATGCTCCTCAACAAGCCACACCAAGAAGACCATCGCCCTCCAACTGTGCGCACCAGAGCAGCCCTGAAGCGCCGAAGACAGTGCCACCATTCCCACGTTTGTTTTGCCACAACCATTCTTGCCTTTGCGGAAGGAGGCGTTTCATTGAAGCAGCCGCCACATCTCTGCTCCCTCTATGTCCTTCAATGGCTTCTTCTAATTCATCTTCTGATTACACTGCTGGACTGAACAGGGTTCATCCTCCTAAGCCAGACTGGTACGAGGATTTCTATGCGTCTGTTCTGGCTAATGGCATGAAATTGTATGAAGCGGAGATTGCAGATTACAAGTCCCAGATGTTTTCCAATTTGAGAGGGAAGGCCCAGAAAGTCTTGGAGATTGGAATCGGTACAGGGCCTAATCTTAAATATTACGCTGGGTATGAAGGTATGAAGGTTTATGGTGTGGATCCAAATCTAAAGATGGAGAAATATGCTCGGGAAGCAGCAAAGAATGCTGGCCTTCCAGCTGAGAATTTTGAGTTTAAACAAGCGGTCGGGGAGGCTATACCATTACCCGATGCTTCTGTCGATGCTGTTGTTGGAACACTTGTCTTGTGTTCTGTGACGAATGTTGACATGACTCTTAAGGAGGTGAAGAGAGTTCTGAGACCCGGCGGACTATACATATTTGTCGAACATGTGGCTGCAAAAGATGGAACCGTGCTTCGATTTATTCAGGACGTGCTCGATCCTCTGCAGCAGATAGTTTCTGATGGATGTCACCTTACCCGGAGGACAGGGCAGAGTATAACAGAAGCAGGGTTTTCAAATGTTGATCTAGACATGGCTTCCTTCTCTAATGCTGCCTTCATAAATCCTCAAGTTTATGGAGTTGCTTATAGATGA

Protein sequence

MSESTVRFQIFLPPLFYRSAITHSLGINNWAKSGNKNDKQQNSSNMILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEAPKTVPPFPRLFCHNHSCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANGMKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKYAREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRPGGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMASFSNAAFINPQVYGVAYR
Homology
BLAST of Lcy13g003060 vs. ExPASy Swiss-Prot
Match: Q9H8H3 (Methyltransferase-like protein 7A OS=Homo sapiens OX=9606 GN=METTL7A PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.6e-29
Identity = 84/203 (41.38%), Postives = 120/203 (59.11%), Query Frame = 0

Query: 162 LYEAEIADYKSQMFSNLR---GKAQK--VLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKM 221
           +Y  ++A  K ++FSNL+   G + K  +LE+G GTG N K+Y    G +V  +DPN   
Sbjct: 46  IYNEQMASKKRELFSNLQEFAGPSGKLSLLEVGCGTGANFKFYP--PGCRVTCIDPNPNF 105

Query: 222 EKY-AREAAKNAGLPAENFEFKQAVGEAI-PLPDASVDAVVGTLVLCSVTNVDMTLKEVK 281
           EK+  +  A+N  L  E F    A GE +  + D SVD VV TLVLCSV N +  L+EV 
Sbjct: 106 EKFLIKSIAENRHLQFERFVV--AAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVC 165

Query: 282 RVLRPGGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVD 341
           RVLRPGG + F+EHVAA+  T   F Q VLDP   ++ DGC+LTR + +++  A FS + 
Sbjct: 166 RVLRPGGAFYFMEHVAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALERASFSKLK 225

Query: 342 LD-MASFSNAAFINPQVYGVAYR 357
           L  + +  +   + P +YG A +
Sbjct: 226 LQHIQAPLSWELVRPHIYGYAVK 244

BLAST of Lcy13g003060 vs. ExPASy Swiss-Prot
Match: Q9DD20 (Methyltransferase-like protein 7B OS=Mus musculus OX=10090 GN=Mettl7b PE=1 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 9.8e-27
Identity = 83/194 (42.78%), Postives = 112/194 (57.73%), Query Frame = 0

Query: 147 WYEDFYASVLANGMKLYEAEIADYKSQMFSNLRGKAQKV--LEIGIGTGPNLKYYAGYEG 206
           ++  F A + A   K  E++  +  SQ+  +L+G +  V  LE+G GTG N ++Y   +G
Sbjct: 35  YFPYFMAMLTARSYKKMESKKRELFSQI-KDLKGTSGNVALLELGCGTGANFQFYP--QG 94

Query: 207 MKVYGVDPNLKMEKY-AREAAKNAGLPAENFEFKQAVGEAI-PLPDASVDAVVGTLVLCS 266
            KV  VDPN   EK+  +  A+N  L  E F    A GE +  L D+S+D VV TLVLCS
Sbjct: 95  CKVTCVDPNPNFEKFLTKSMAENRHLQYERFIV--AYGENMKQLADSSMDVVVCTLVLCS 154

Query: 267 VTNVDMTLKEVKRVLRPGGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTG 326
           V +    L+EV+RVLRPGGL  F EHVA   G+     Q VL+P  + + DGCHLTR T 
Sbjct: 155 VQSPRKVLQEVQRVLRPGGLLFFWEHVAEPQGSRAFLWQRVLEPTWKHIGDGCHLTRETW 214

Query: 327 QSITEAGFSNVDLD 337
           + I  A FS V L+
Sbjct: 215 KDIERAQFSEVQLE 223

BLAST of Lcy13g003060 vs. ExPASy Swiss-Prot
Match: Q562C4 (Methyltransferase-like protein 7B OS=Rattus norvegicus OX=10116 GN=Mettl7b PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.9e-26
Identity = 80/188 (42.55%), Postives = 111/188 (59.04%), Query Frame = 0

Query: 153 ASVLANGMKLYEAEIADYKSQMFSNLRGKAQKV--LEIGIGTGPNLKYYAGYEGMKVYGV 212
           A++ A   K  E++  +  SQ+  +L+G + +V  LE+G GTG N ++Y    G KV  V
Sbjct: 41  ATLTARSYKKMESKKRELFSQI-KDLKGTSNEVTLLELGCGTGANFQFYP--PGCKVTCV 100

Query: 213 DPNLKMEKY-AREAAKNAGLPAENFEFKQAVGEAI-PLPDASVDAVVGTLVLCSVTNVDM 272
           DPN   EK+  +  A+N  L  E F    A GE +  L D+S+D VV TLVLCSV +   
Sbjct: 101 DPNPNFEKFLTKSMAENRHLQYERFIV--AYGENMKQLADSSMDVVVCTLVLCSVQSPRK 160

Query: 273 TLKEVKRVLRPGGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEA 332
            L+EV+RVL+PGGL  F EHV+   G+     Q VL+P  + + DGCHLTR T + I +A
Sbjct: 161 VLQEVQRVLKPGGLLFFWEHVSEPQGSQALLWQRVLEPTWKHIGDGCHLTRETWKDIEKA 220

Query: 333 GFSNVDLD 337
            FS V L+
Sbjct: 221 QFSEVQLE 223

BLAST of Lcy13g003060 vs. ExPASy Swiss-Prot
Match: Q6UX53 (Methyltransferase-like protein 7B OS=Homo sapiens OX=9606 GN=METTL7B PE=1 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 7.3e-22
Identity = 69/173 (39.88%), Postives = 97/173 (56.07%), Query Frame = 0

Query: 171 KSQMFSNLRGKA-----QKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY-AREAA 230
           K ++FS ++G         +LE+G GTG N ++Y    G +V  +DPN   EK+  +  A
Sbjct: 55  KRELFSQIKGLTGASGKVALLELGCGTGANFQFYP--PGCRVTCLDPNPHFEKFLTKSMA 114

Query: 231 KNAGLPAENFEFKQAVGEAI-PLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRPGGLY 290
           +N  L  E F    A GE +  L D S+D VV TLVLCSV +    L+EV+RVLRPGG+ 
Sbjct: 115 ENRHLQYERFVV--APGEDMRQLADGSMDVVVCTLVLCSVQSPRKVLQEVRRVLRPGGVL 174

Query: 291 IFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLD 337
            F EHVA   G+     Q V +P  + + DGC LTR T + +  A FS + ++
Sbjct: 175 FFWEHVAEPYGSWAFMWQQVFEPTWKHIGDGCCLTRETWKDLENAQFSEIQME 223

BLAST of Lcy13g003060 vs. ExPASy Swiss-Prot
Match: A7MQL7 (Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE OS=Cronobacter sakazakii (strain ATCC BAA-894) OX=290339 GN=ubiE PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-08
Identity = 47/150 (31.33%), Postives = 74/150 (49.33%), Query Frame = 0

Query: 181 KAQKVLEIGIGTGPNLKYYAGY--EGMKVYGVDPNLKMEKYAREAAKNAGLPAENFEFKQ 240
           + QKVL++  GTG     ++    E  KV   D N  M K  RE  +N G+   N E+ Q
Sbjct: 63  RGQKVLDLAGGTGDLTAKFSRLVGESGKVVLADINDSMLKMGREKLRNIGI-VGNVEYVQ 122

Query: 241 AVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRPGGLYIFVEHVAAKDGTVLR 300
           A  EA+P PD + D +  +  L +VT+ +  L+ + RVL+PGG  +           VL 
Sbjct: 123 ANAEALPFPDNTFDCITISFGLRNVTDKEKALRSMFRVLKPGGRLL-----------VLE 182

Query: 301 FIQDVLDPLQQIV-SDGCHLTRRTGQSITE 328
           F + V +PL +   +   H+  R G+ + +
Sbjct: 183 FSKPVFEPLNKAYDAYSFHILPRVGELVAK 200

BLAST of Lcy13g003060 vs. ExPASy TrEMBL
Match: A0A6J1G0D7 (methyltransferase-like protein 7A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449575 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 3.2e-153
Identity = 280/317 (88.33%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYASPPSSIIFSKRNAPQQATPR PSPSNCA QSS EA     PK + PFPRLFCHN 
Sbjct: 1   MILYASPPSSIIFSKRNAPQQATPRTPSPSNCARQSSQEAPNGANPKPLSPFPRLFCHNR 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYTA LNRVHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTAILNRVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA+NAGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQNAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA++GTVLRF+QDVLDPLQQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 NGLYIFVEHVAAEEGTVLRFVQDVLDPLQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQV+GVA+R
Sbjct: 301 FSNAAFINPQVFGVAHR 317

BLAST of Lcy13g003060 vs. ExPASy TrEMBL
Match: A0A6J1G0K4 (methyltransferase-like protein 7A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449575 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 1.0e-151
Identity = 280/317 (88.33%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYASPPSSIIFSKRNAPQQATPR PSPSNCA QSS EA     PK + PFPRLFCHN 
Sbjct: 1   MILYASPPSSIIFSKRNAPQQATPRTPSPSNCARQSSQEAPNGANPKPLSPFPRLFCHNR 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYTA LNRVHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTAILNRVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA+NAGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQNAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA +GTVLRF+QDVLDPLQQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 NGLYIFVEHVAA-EGTVLRFVQDVLDPLQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQV+GVA+R
Sbjct: 301 FSNAAFINPQVFGVAHR 316

BLAST of Lcy13g003060 vs. ExPASy TrEMBL
Match: A0A6J1HSK1 (methyltransferase-like protein 7A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467423 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.7e-151
Identity = 277/317 (87.38%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYAS PSSIIFSKRNAPQQATPRRPSP NCA QSS +A     PK +  FPRLFCHNH
Sbjct: 1   MILYASTPSSIIFSKRNAPQQATPRRPSPFNCARQSSQDAPNGGNPKPLSSFPRLFCHNH 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYTA LNRVHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTAILNRVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA++AGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQSAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA++GTVLRF+QDVLDP+QQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 SGLYIFVEHVAAEEGTVLRFVQDVLDPVQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQVYGVA+R
Sbjct: 301 FSNAAFINPQVYGVAHR 317

BLAST of Lcy13g003060 vs. ExPASy TrEMBL
Match: A0A6J1HYL0 (methyltransferase-like protein 7A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467423 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 5.6e-150
Identity = 277/317 (87.38%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYAS PSSIIFSKRNAPQQATPRRPSP NCA QSS +A     PK +  FPRLFCHNH
Sbjct: 1   MILYASTPSSIIFSKRNAPQQATPRRPSPFNCARQSSQDAPNGGNPKPLSSFPRLFCHNH 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYTA LNRVHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTAILNRVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA++AGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQSAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA +GTVLRF+QDVLDP+QQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 SGLYIFVEHVAA-EGTVLRFVQDVLDPVQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQVYGVA+R
Sbjct: 301 FSNAAFINPQVYGVAHR 316

BLAST of Lcy13g003060 vs. ExPASy TrEMBL
Match: A0A0A0KW08 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606280 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.2e-146
Identity = 272/312 (87.18%), Postives = 283/312 (90.71%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEAPKTVPPFPRLFCHNHSCLCG 105
           MILYASPPSS IFS  NA QQA P R S SNCAHQS PEA    PPFPRLFCHNHSCLCG
Sbjct: 1   MILYASPPSSAIFSISNASQQAKP-RSSSSNCAHQSIPEA----PPFPRLFCHNHSCLCG 60

Query: 106 RRRFIEAA-ATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANGMKLYE 165
           RRRFIEAA ATSL PLCPSMASSNS SDY A LNRV  PKPDWYEDFYASVLANGMK YE
Sbjct: 61  RRRFIEAATATSLFPLCPSMASSNSPSDYAAILNRVRSPKPDWYEDFYASVLANGMKSYE 120

Query: 166 AEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKYAREAA 225
            EIA YKSQMF+NLRGK+QKVLEIGIG GPNLKYYAG EGM+VYGVDPN KMEKYAREAA
Sbjct: 121 EEIAVYKSQMFANLRGKSQKVLEIGIGAGPNLKYYAGNEGMEVYGVDPNQKMEKYAREAA 180

Query: 226 KNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRPGGLYI 285
           KNAGLP E+FEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTL+EVKRVLRPGGLYI
Sbjct: 181 KNAGLPPESFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLREVKRVLRPGGLYI 240

Query: 286 FVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMASFSNAA 345
           FVEHVAAK+GTVLRFIQDVLDPLQQIV+DGCHLTRRTGQ+I + GFSNVDL++ASFSNAA
Sbjct: 241 FVEHVAAKEGTVLRFIQDVLDPLQQIVADGCHLTRRTGQNIIQTGFSNVDLNIASFSNAA 300

Query: 346 FINPQVYGVAYR 357
           FINPQVYGVAYR
Sbjct: 301 FINPQVYGVAYR 307

BLAST of Lcy13g003060 vs. NCBI nr
Match: KAG7013130.1 (Methyltransferase-like protein 7A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 555.1 bits (1429), Expect = 4.5e-154
Identity = 282/317 (88.96%), Postives = 295/317 (93.06%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYASPPSSIIFSKRNAPQQATPRRPSPSNCA QSS EA     PK + PFPRLFCHN 
Sbjct: 1   MILYASPPSSIIFSKRNAPQQATPRRPSPSNCARQSSQEAPNGANPKPLSPFPRLFCHNR 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYTA LNRVHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTAILNRVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA+NAGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQNAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA++GTVLRF+QDVLDPLQQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 NGLYIFVEHVAAEEGTVLRFVQDVLDPLQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQVYGVA+R
Sbjct: 301 FSNAAFINPQVYGVAHR 317

BLAST of Lcy13g003060 vs. NCBI nr
Match: XP_023542970.1 (methyltransferase-like protein 7A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 552.4 bits (1422), Expect = 2.9e-153
Identity = 280/317 (88.33%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYASPPSSIIFSKRNAPQQATP RPSPSNCA QSS EA     PK + PFPRLFCHNH
Sbjct: 1   MILYASPPSSIIFSKRNAPQQATPIRPSPSNCARQSSQEAPNGANPKPLSPFPRLFCHNH 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYT  LN+VHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTTILNKVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA+NAGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQNAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA++GTVLRF+QDVLDPLQQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 NGLYIFVEHVAAEEGTVLRFVQDVLDPLQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQVYGVA+R
Sbjct: 301 FSNAAFINPQVYGVAHR 317

BLAST of Lcy13g003060 vs. NCBI nr
Match: XP_022945283.1 (methyltransferase-like protein 7A isoform X1 [Cucurbita moschata])

HSP 1 Score: 551.2 bits (1419), Expect = 6.5e-153
Identity = 280/317 (88.33%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYASPPSSIIFSKRNAPQQATPR PSPSNCA QSS EA     PK + PFPRLFCHN 
Sbjct: 1   MILYASPPSSIIFSKRNAPQQATPRTPSPSNCARQSSQEAPNGANPKPLSPFPRLFCHNR 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYTA LNRVHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTAILNRVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA+NAGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQNAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA++GTVLRF+QDVLDPLQQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 NGLYIFVEHVAAEEGTVLRFVQDVLDPLQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQV+GVA+R
Sbjct: 301 FSNAAFINPQVFGVAHR 317

BLAST of Lcy13g003060 vs. NCBI nr
Match: XP_023542971.1 (methyltransferase-like protein 7A isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.4 bits (1409), Expect = 9.4e-152
Identity = 280/317 (88.33%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYASPPSSIIFSKRNAPQQATP RPSPSNCA QSS EA     PK + PFPRLFCHNH
Sbjct: 1   MILYASPPSSIIFSKRNAPQQATPIRPSPSNCARQSSQEAPNGANPKPLSPFPRLFCHNH 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYT  LN+VHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTTILNKVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA+NAGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQNAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA +GTVLRF+QDVLDPLQQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 NGLYIFVEHVAA-EGTVLRFVQDVLDPLQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQVYGVA+R
Sbjct: 301 FSNAAFINPQVYGVAHR 316

BLAST of Lcy13g003060 vs. NCBI nr
Match: XP_022945284.1 (methyltransferase-like protein 7A isoform X2 [Cucurbita moschata])

HSP 1 Score: 546.2 bits (1406), Expect = 2.1e-151
Identity = 280/317 (88.33%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 46  MILYASPPSSIIFSKRNAPQQATPRRPSPSNCAHQSSPEA-----PKTVPPFPRLFCHNH 105
           MILYASPPSSIIFSKRNAPQQATPR PSPSNCA QSS EA     PK + PFPRLFCHN 
Sbjct: 1   MILYASPPSSIIFSKRNAPQQATPRTPSPSNCARQSSQEAPNGANPKPLSPFPRLFCHNR 60

Query: 106 -SCLCGRRRFIEAAATSLLPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 165
            SC+CGRR FIEAAATSL PL  SMASSNSSSDYTA LNRVHPPKPDWYEDFYASVLANG
Sbjct: 61  CSCICGRRLFIEAAATSLFPLSSSMASSNSSSDYTAILNRVHPPKPDWYEDFYASVLANG 120

Query: 166 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 225
           MKLYEAEIADYKSQMF+ LRGKAQKVLEIGIG GPNLKYYAG EGMKVYGVDPN KMEKY
Sbjct: 121 MKLYEAEIADYKSQMFATLRGKAQKVLEIGIGAGPNLKYYAGEEGMKVYGVDPNPKMEKY 180

Query: 226 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 285
           AREAA+NAGLPA+NFEFKQAVGEAIPLPDASVD VVGTLVLCSV+NVDMTLKEVKRVLRP
Sbjct: 181 AREAAQNAGLPADNFEFKQAVGEAIPLPDASVDVVVGTLVLCSVSNVDMTLKEVKRVLRP 240

Query: 286 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMAS 345
            GLYIFVEHVAA +GTVLRF+QDVLDPLQQ+VSDGCHLTRRTGQSI+EAGFSNVDL+MAS
Sbjct: 241 NGLYIFVEHVAA-EGTVLRFVQDVLDPLQQLVSDGCHLTRRTGQSISEAGFSNVDLNMAS 300

Query: 346 FSNAAFINPQVYGVAYR 357
           FSNAAFINPQV+GVA+R
Sbjct: 301 FSNAAFINPQVFGVAHR 316

BLAST of Lcy13g003060 vs. TAIR 10
Match: AT1G69523.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 4.7e-85
Identity = 157/257 (61.09%), Postives = 193/257 (75.10%), Query Frame = 0

Query: 102 CLCGRRRFIEAAATSL--LPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 161
           C CGRR FI AA TS+  LP+ PS AS++     T  L R+ PPKPDWYE+F+A  + + 
Sbjct: 48  CPCGRRHFIGAAMTSMPFLPISPSHASTS-----TEDLKRLRPPKPDWYEEFFAWSMNSE 107

Query: 162 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 221
           ++ YE E++DYK ++F NL GKA+KVLEIGIGTGPN KYY     + V G+DPN KME Y
Sbjct: 108 VESYEKEVSDYKMKLFDNLVGKAEKVLEIGIGTGPNFKYYTDIPNVSVIGIDPNAKMESY 167

Query: 222 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 281
           AR++A  AGL  E F F  A+GE+IPL DASVDAVVGTLVLCSVT+V  TLKE+KR+LRP
Sbjct: 168 ARKSATEAGLKPEEFTFVHALGESIPLEDASVDAVVGTLVLCSVTDVTQTLKEIKRILRP 227

Query: 282 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFS-NVDLDMA 341
           GG+YIF+EHVAA+DGT LR +Q VLDPLQQ+V+DGCHLTR TG+SI EA F+   D+   
Sbjct: 228 GGIYIFIEHVAAEDGTFLRLVQTVLDPLQQVVADGCHLTRHTGESILEARFNGGADVKKT 287

Query: 342 SFSNAAFINPQVYGVAY 356
           S S  A+I+  VYGVAY
Sbjct: 288 SLSRLAYISSHVYGVAY 299

BLAST of Lcy13g003060 vs. TAIR 10
Match: AT1G69526.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.8e-77
Identity = 142/259 (54.83%), Postives = 186/259 (71.81%), Query Frame = 0

Query: 102 CLCGRRRFIEAAATSL--LPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 161
           C CGR+ F+EAA+ ++  LP+C   AS +     T      HP +PDWY++ +A  L+ G
Sbjct: 54  CSCGRKHFLEAASPTMPFLPICSPNASRSKDVSET-----FHPQRPDWYKELFAWFLSTG 113

Query: 162 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 221
           M+ YEAEIA+YK ++F  L GKA+ VLEIG+GTGPNLKY+AG E + V+G+DPN KMEKY
Sbjct: 114 MRSYEAEIAEYKRKLFEKLAGKAETVLEIGVGTGPNLKYFAGNENVCVFGMDPNHKMEKY 173

Query: 222 AREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLRP 281
           A E+A+ AG+  ENF F   VGEAIPL D S+D+VV TLVLCSV++V  TL E+KRVL+P
Sbjct: 174 ACESAREAGMKPENFRFMHGVGEAIPLDDDSMDSVVATLVLCSVSDVTQTLNEIKRVLKP 233

Query: 282 GGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVD--LDM 341
           GG+++F+EHVAAKDG+  R +Q+VLDP+QQ+V+DGCHLTR T   I+ AGF       D 
Sbjct: 234 GGIFLFIEHVAAKDGSFFRHVQNVLDPIQQVVADGCHLTRNTDLHISAAGFDGGTEINDT 293

Query: 342 ASFSNAAFINPQVYGVAYR 357
           A +S    I P VYG AY+
Sbjct: 294 AIYSFPWIIRPHVYGAAYK 307

BLAST of Lcy13g003060 vs. TAIR 10
Match: AT1G69520.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 6.5e-58
Identity = 116/200 (58.00%), Postives = 149/200 (74.50%), Query Frame = 0

Query: 160 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAG-YEGMKVYGVDPNLKMEK 219
           M+ Y  EI D K ++F  L  KA+KVLEIGIGTGPN++YYA     + +YG+DPN KM+K
Sbjct: 32  MQSYWNEIEDCKVKIFDKLSEKAEKVLEIGIGTGPNMRYYAARNSNVTLYGLDPNPKMKK 91

Query: 220 YAREAAKNAGLPAENFEFKQAVGEAIPLPDASVDAVVGTLVLCSVTNVDMTLKEVKRVLR 279
           YAR++A  AGL  +NF FKQ VGEAIPL D SVDAVV TLVLCSV++V  TLKE+KRVLR
Sbjct: 92  YARKSATKAGLKPKNFRFKQGVGEAIPLKDNSVDAVVATLVLCSVSDVTQTLKEIKRVLR 151

Query: 280 PGGLYIFVEHVAAKDGTVLRFIQDVLDPLQQIVSDGCHLTRRTGQSITEAGFSNVDLDMA 339
            GG++IF+EHVAAKDG+  + +Q +LDPLQQ ++DGCHL R T + I EAGFS   +++ 
Sbjct: 152 QGGVFIFLEHVAAKDGSFFKRLQKLLDPLQQRLADGCHLARNTRECILEAGFSG-GVEVQ 211

Query: 340 SFSNAAF---INPQVYGVAY 356
           +FS  +F     P +YG+AY
Sbjct: 212 TFSMYSFPWMTRPHIYGLAY 230

BLAST of Lcy13g003060 vs. TAIR 10
Match: AT1G69526.3 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 1.8e-36
Identity = 73/145 (50.34%), Postives = 100/145 (68.97%), Query Frame = 0

Query: 102 CLCGRRRFIEAAATSL--LPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 161
           C CGR+ F+EAA+ ++  LP+C   AS +     T      HP +PDWY++ +A  L+ G
Sbjct: 54  CSCGRKHFLEAASPTMPFLPICSPNASRSKDVSET-----FHPQRPDWYKELFAWFLSTG 113

Query: 162 MKLYEAEIADYKSQMFSNLRGKAQKVLEIGIGTGPNLKYYAGYEGMKVYGVDPNLKMEKY 221
           M+ YEAEIA+YK ++F  L GKA+ VLEIG+GTGPNLKY+AG E + V+G+DPN KMEKY
Sbjct: 114 MRSYEAEIAEYKRKLFEKLAGKAETVLEIGVGTGPNLKYFAGNENVCVFGMDPNHKMEKY 173

Query: 222 AREAAKNAGLPAENFEFKQAVGEAI 245
           A E+A+ AG+  ENF F   V + +
Sbjct: 174 ACESAREAGMKPENFRFMHGVSKLL 193

BLAST of Lcy13g003060 vs. TAIR 10
Match: AT1G69526.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.4e-31
Identity = 73/176 (41.48%), Postives = 100/176 (56.82%), Query Frame = 0

Query: 102 CLCGRRRFIEAAATSL--LPLCPSMASSNSSSDYTAGLNRVHPPKPDWYEDFYASVLANG 161
           C CGR+ F+EAA+ ++  LP+C   AS +     T      HP +PDWY++ +A  L+ G
Sbjct: 54  CSCGRKHFLEAASPTMPFLPICSPNASRSKDVSET-----FHPQRPDWYKELFAWFLSTG 113

Query: 162 MKLYEAE-------------------------------IADYKSQMFSNLRGKAQKVLEI 221
           M+ YEAE                               IA+YK ++F  L GKA+ VLEI
Sbjct: 114 MRSYEAEVSLCDLNASFYDPFLENDRSFSCFMFVLFGKIAEYKRKLFEKLAGKAETVLEI 173

Query: 222 GIGTGPNLKYYAGYEGMKVYGVDPNLKMEKYAREAAKNAGLPAENFEFKQAVGEAI 245
           G+GTGPNLKY+AG E + V+G+DPN KMEKYA E+A+ AG+  ENF F   V + +
Sbjct: 174 GVGTGPNLKYFAGNENVCVFGMDPNHKMEKYACESAREAGMKPENFRFMHGVSKLL 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9H8H33.6e-2941.38Methyltransferase-like protein 7A OS=Homo sapiens OX=9606 GN=METTL7A PE=1 SV=1[more]
Q9DD209.8e-2742.78Methyltransferase-like protein 7B OS=Mus musculus OX=10090 GN=Mettl7b PE=1 SV=2[more]
Q562C44.9e-2642.55Methyltransferase-like protein 7B OS=Rattus norvegicus OX=10116 GN=Mettl7b PE=1 ... [more]
Q6UX537.3e-2239.88Methyltransferase-like protein 7B OS=Homo sapiens OX=9606 GN=METTL7B PE=1 SV=2[more]
A7MQL71.2e-0831.33Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE OS=Cronobacter saka... [more]
Match NameE-valueIdentityDescription
A0A6J1G0D73.2e-15388.33methyltransferase-like protein 7A isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1G0K41.0e-15188.33methyltransferase-like protein 7A isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1HSK11.7e-15187.38methyltransferase-like protein 7A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1HYL05.6e-15087.38methyltransferase-like protein 7A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0KW082.2e-14687.18Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G60... [more]
Match NameE-valueIdentityDescription
KAG7013130.14.5e-15488.96Methyltransferase-like protein 7A [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023542970.12.9e-15388.33methyltransferase-like protein 7A isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022945283.16.5e-15388.33methyltransferase-like protein 7A isoform X1 [Cucurbita moschata][more]
XP_023542971.19.4e-15288.33methyltransferase-like protein 7A isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022945284.12.1e-15188.33methyltransferase-like protein 7A isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G69523.14.7e-8561.09S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G69526.22.8e-7754.83S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G69520.16.5e-5858.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G69526.31.8e-3650.34S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G69526.11.4e-3141.48S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 152..348
e-value: 3.5E-30
score: 107.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..83
NoneNo IPR availablePANTHERPTHR45036METHYLTRANSFERASE LIKE 7Bcoord: 70..355
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 184..286
e-value: 1.57206E-13
score: 64.3734
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 186..285
e-value: 2.5E-21
score: 76.1
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 148..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g003060.1Lcy13g003060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity