Lcy13g002470 (gene) Sponge gourd (P93075) v1

BLAST of Lcy13g002470 vs. ExPASy Swiss-Prot
Match: Q9LFY0 (Protein PTST homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST2 PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 6.2e-94
Identity = 216/474 (45.57%), Postives = 291/474 (61.39%), Query Frame = 0

Query: 63  CSGFLRRCK----GLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVK 122
           C+G  R C     G DSEG   LE EIL+FM++S  P  FPSKKDLI +GR DLV+ IV 
Sbjct: 72  CNGVSRMCNKRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVN 131

Query: 123 EGGWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIA 182
           +GGWL +GW LDE+E+        + R +   T  +  ++      +  +   T      
Sbjct: 132 QGGWLSMGWDLDEQEE--------KVRVNENVTPQDLHIEKQLPNCNSPEMDKTLNH--G 191

Query: 183 SLSTSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQET 242
            L  SS+ +  + +  + N+SGIEG+L+RLEKERN++ G         N    + +   +
Sbjct: 192 DLDLSSNLSSSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGS 251

Query: 243 VADLQGSTISTPLSAKEGKWNDLGGHLGQN--SSPVQINGLGGLL-SPEVQRLRTWSTFK 302
           V     S I T    +E   +   G   Q+       ++G  GL  SP  +  RTWS  +
Sbjct: 252 VP--WSSRIVTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRR 311

Query: 303 TELQDGDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNL 362
               D DFEA+EI+SS G +    D T+ ++  S           E ++   I+  LQ L
Sbjct: 312 AGFTDEDFEAAEISSS-GLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQQL 371

Query: 363 ELELSSVVGSLRS--NALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIA 422
           + ELSSV+ SLRS  + + + K    ++  L  LSD +E++ENEI++A++KLRS RAK+A
Sbjct: 372 QSELSSVLHSLRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLA 431

Query: 423 VVEGKMALTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQ 482
           V+EGKMA+ IIDAQ+++ EKQ+R++ A RAL+LLRTA +VWPNSASEVLL GSFDGWSTQ
Sbjct: 432 VLEGKMAMAIIDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWSTQ 491

Query: 483 RKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           RKM+K+  GVFSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLII+
Sbjct: 492 RKMKKAENGVFSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of Lcy13g002470 vs. ExPASy Swiss-Prot
Match: Q10F03 (Protein FLOURY ENDOSPERM 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FLO6 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.1e-65
Identity = 178/481 (37.01%), Postives = 259/481 (53.85%), Query Frame = 0

Query: 75  SEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEGGWLCLGWSLDEEED 134
           + G+  LE  I +FM  S  P AFP++ +L+ AGR DL  A+   GGWL LGWS    E 
Sbjct: 76  ARGEEDLEEAIYEFMRRSDKPGAFPTRAELVAAGRADLAAAVDACGGWLSLGWSSGGAEA 135

Query: 135 G----DIGLFS--LEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASLSTSSSFTG 194
           G     +G+      + G+  A             + G    A      +S    +S +G
Sbjct: 136 GRASSSVGVHPDYPPEAGAAAA-------------AGGASDLAQGAVWASSREAEASPSG 195

Query: 195 RSLETVAENE-----SGIEGMLSRLEKER---NVTFGAGFPAAVGKND---LHPETAAQE 254
           R  ET  E       +G++GML+RL++ER             A G+ D   L  ++ A  
Sbjct: 196 RQPETEEEETETKFGTGLDGMLTRLQRERERVRPPLPRSSDGAGGERDNVALMGQSGAPS 255

Query: 255 TVADLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTE 314
             A     T   P +     ++   G L  N S   +              RTWS  K  
Sbjct: 256 HSATGGRYTPKVPDNGNIHSYHPQNGALEHNKSSKSLTN---------DAWRTWSLDKGG 315

Query: 315 LQDGDFEASEIASSKGRS---EEVLDVTRYETLGSRWDPTNS------NFGEEKMDLNQI 374
               DF+A+EI S+  R     + LD+   + +     P+N       +  +   + + I
Sbjct: 316 F--SDFQAAEIHSTNSRKSFRHDGLDILAQDDVHG---PSNGVAVHDYDINDVDSERDDI 375

Query: 375 RSYLQNLELELSSVVGSLRS--NALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLR 434
            + LQNLEL+L++ + +LRS  + + S+  +   +     LSD +EF+E +++ A+++LR
Sbjct: 376 HARLQNLELDLTAALHTLRSRFDKVISDMSEGDGAKAPNGLSDDWEFEETKVMQAQEELR 435

Query: 435 SIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGS 494
           SIRAKIAV+EGKMAL II+  K+IEEKQ+R++ A +AL  LRT  +VW N ASEVLL GS
Sbjct: 436 SIRAKIAVLEGKMALEIIEKNKIIEEKQRRLDEAEKALSELRTVYIVWSNPASEVLLTGS 495

Query: 495 FDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLII 528
           FDGW++QR+ME+S  G FSL L+LYPG+YEIKFIVDG W+ DPLRP+V+++G+ENNLL +
Sbjct: 496 FDGWTSQRRMERSERGTFSLNLRLYPGRYEIKFIVDGVWRNDPLRPLVSNNGHENNLLTV 529

BLAST of Lcy13g002470 vs. ExPASy Swiss-Prot
Match: F4KFB3 (Protein PTST homolog 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.7e-12
Identity = 46/148 (31.08%), Postives = 75/148 (50.68%), Query Frame = 0

Query: 383 ELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARR 442
           E+  L    + +E E+   K+++   +  ++V++ +    I  AQ +I EK+  +  A  
Sbjct: 446 EIDRLRFMLDQKELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEE 505

Query: 443 ALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSST------GVFSLFLKLYPGKYE 502
           +L  L+   + +    + V + GSF+GW  +  ME  ++        +S  L LYPG YE
Sbjct: 506 SLSGLQEVVIEYCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYE 565

Query: 503 IKFIVDGQWKIDPLRPIVNSSGYENNLL 525
           IKFIVDGQW  DP +  V      NN+L
Sbjct: 566 IKFIVDGQWITDPQKDSVTRGHISNNIL 593

BLAST of Lcy13g002470 vs. ExPASy Swiss-Prot
Match: Q94AX2 (Protein PTST, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.0e-11
Identity = 40/110 (36.36%), Postives = 63/110 (57.27%), Query Frame = 0

Query: 421 LTIIDA-QKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKS 480
           L+ +DA QK ++E+ K +  A+      +   V W   A  V ++GSFDGWS +  +   
Sbjct: 172 LSRLDAVQKKMKEQIKGVEAAQS-----KEVHVFWIGMAESVQVMGSFDGWSQREDLSPE 231

Query: 481 STGVFSLF---LKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLII 527
            + +F+ F   L L PG+YE+KF+VDG+W+I P  P       ENN+L++
Sbjct: 232 YSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVV 276

BLAST of Lcy13g002470 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.0e-08
Identity = 34/92 (36.96%), Postives = 49/92 (53.26%), Query Frame = 0

Query: 434 QKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPG 493
           Q+ ++ + +  Q  R   + W     EV + GSF+ WST+  + KS    F   L L  G
Sbjct: 62  QQDLDDSVKPAQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHND-FVAILDLPEG 121

Query: 494 KYEIKFIVDGQWKIDPLRPIVNSS-GYENNLL 525
           +++ KF VDGQW  DP  P+V S  G  NNL+
Sbjct: 122 EHQYKFFVDGQWVHDPSEPVVTSQLGTINNLI 152

BLAST of Lcy13g002470 vs. ExPASy TrEMBL
Match: A0A6J1HXH5 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 2.0e-236
Identity = 431/527 (81.78%), Postives = 465/527 (88.24%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           MLLFTNALPTSYF PT FGCLSPC FPS VFV  SRCG NS HMAATER C  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSGF+RRCKGLDSEG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSD RQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETVA 240
           STSSSFT RS ETVAE+ESGIEGML+RLE+ERN TFG       GKN LH ET A+ETVA
Sbjct: 181 STSSSFTSRSQETVAEHESGIEGMLNRLERERNATFG------TGKNGLHSETTAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKW +LG +L +NSSPVQING GG +S EVQ  RTWSTFKT+LQD
Sbjct: 241 DLQEIPISTPLSAKEGKWTNLGSYLSKNSSPVQINGQGGSVSSEVQ--RTWSTFKTDLQD 300

Query: 301 GDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ SE+A S+ RS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ Y+QNL+LELS
Sbjct: 301 GDFKGSEMALSEVRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRYIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S +GSLRSNA ASEKGQS+ SD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SAIGSLRSNAFASEKGQSKPSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LRTACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV+S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVSSDGYENNLLIVT 517

BLAST of Lcy13g002470 vs. ExPASy TrEMBL
Match: A0A6J1DCK2 (uncharacterized protein LOC111019111 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019111 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.3e-232
Identity = 418/527 (79.32%), Postives = 460/527 (87.29%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           ML+FTNALPT  F P+ FGCLSPCVFP++VFVSNSRCGWNS H AATER CV LSSKGMF
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSG +RRCKGLDSEGDFALEA+IL+FM+SS+NPEAFPSKKDLIEAGREDLVDAI K+G
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+LDEE D  +G +  EDR SI+ATDW+SF +SN+ + D R RFA A SP+AS 
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETVA 240
           S SSSFTGRSLE VAE ESGIEGMLSRLEKERNVTFGAGFP             A+ETVA
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGAGFP------------GAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQGS+ISTPLSAKEGKWN+LGG L  NSSPVQINGLGG +SPEVQR+  W+T+ TEL+D
Sbjct: 241 DLQGSSISTPLSAKEGKWNNLGGSLSHNSSPVQINGLGGSVSPEVQRI--WNTYNTELRD 300

Query: 301 GDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDFEASE A   GR  EVLDV+RY TLGS  D   SN GEE+M+ +QIR +LQNLELELS
Sbjct: 301 GDFEASENALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S+VGSLRSN  ASEKGQSRS+D+ LELSDA+EFQENEILNAKDKLRSIRAK+AVVEGKMA
Sbjct: 361 SMVGSLRSNTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           +TIIDAQKVIEEKQKR+NCARRALQ+LRTACVVWPNSASEV LVGSFDGW+TQRKMEKSS
Sbjct: 421 MTIIDAQKVIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           TGVFSLFLKLYPGKYEIKFIVDGQW+IDPLRPIVNSSGYENNLLIIT
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 513

BLAST of Lcy13g002470 vs. ExPASy TrEMBL
Match: A0A6J1HT44 (protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 1.5e-231
Identity = 431/558 (77.24%), Postives = 465/558 (83.33%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           MLLFTNALPTSYF PT FGCLSPC FPS VFV  SRCG NS HMAATER C  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSGF+RRCKGLDSEG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSD RQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRS-------------------------------LETVAENESGIEGMLSRLE 240
           STSSSFT RS                                ETVAE+ESGIEGML+RLE
Sbjct: 181 STSSSFTSRSQVFYSMPMKRNLIFYDNKMLCQDVKDTNFTNRETVAEHESGIEGMLNRLE 240

Query: 241 KERNVTFGAGFPAAVGKNDLHPETAAQETVADLQGSTISTPLSAKEGKWNDLGGHLGQNS 300
           +ERN TFG       GKN LH ET A+ETVADLQ   ISTPLSAKEGKW +LG +L +NS
Sbjct: 241 RERNATFG------TGKNGLHSETTAKETVADLQEIPISTPLSAKEGKWTNLGSYLSKNS 300

Query: 301 SPVQINGLGGLLSPEVQRLRTWSTFKTELQDGDFEASEIASSKGRSEEVLDVTRYETLGS 360
           SPVQING GG +S EVQ  RTWSTFKT+LQDGDF+ SE+A S+ RS EVLDV RYETLGS
Sbjct: 301 SPVQINGQGGSVSSEVQ--RTWSTFKTDLQDGDFKGSEMALSEVRSREVLDVLRYETLGS 360

Query: 361 RWDPTNSNFGEEKMDLNQIRSYLQNLELELSSVVGSLRSNALASEKGQSRSSDELLELSD 420
           R DPTNSN GEE+M  NQI+ Y+QNL+LELSS +GSLRSNA ASEKGQS+ SD+LLELSD
Sbjct: 361 RGDPTNSNSGEEEMSHNQIQRYIQNLQLELSSAIGSLRSNAFASEKGQSKPSDDLLELSD 420

Query: 421 AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARRALQLLRT 480
           AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKV+EEKQKR+NCARRALQ LRT
Sbjct: 421 AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRT 480

Query: 481 ACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP 528
           ACVVWPNSA+EVLLVGSFDGWSTQRKME+SSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP
Sbjct: 481 ACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP 540

BLAST of Lcy13g002470 vs. ExPASy TrEMBL
Match: A0A6J1FZM9 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449347 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.6e-231
Identity = 426/527 (80.83%), Postives = 461/527 (87.48%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           MLLFTN LPTSYF PT FGCLSP VFPSIVFV  SRCG     MAATER C  LSSKGMF
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFV--SRCG----GMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSGF+RRCK LD EG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSD RQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETVA 240
           STSSSFT RS ETV E+ESGIEGML+RLE+ERNVTFG       GKN LHPET  +ETVA
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFG------TGKNCLHPETTVKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKWN+LG +L +NSSPVQING GG +S EVQ   TWSTFKT+L D
Sbjct: 241 DLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQ--GTWSTFKTDLPD 300

Query: 301 GDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ SE+A S+GRS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ ++QNL+LELS
Sbjct: 301 GDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           SV+GSLRSN+ ASEKGQS+SSD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LRTACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 513

BLAST of Lcy13g002470 vs. ExPASy TrEMBL
Match: A0A6J1DBZ3 (uncharacterized protein LOC111019111 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019111 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 4.4e-228
Identity = 414/527 (78.56%), Postives = 456/527 (86.53%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           ML+FTNALPT  F P+ FGCLSPCVFP++VFVSNSRCGWNS H AATER CV LSSKGMF
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSG +RRCKGLDSEGDFALEA+IL+FM+SS+NPEAFPSKKDLIEAGREDLVDAI K+G
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+LDEE D  +G +  EDR SI+ATDW+SF +SN+ + D R RFA A SP+AS 
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETVA 240
           S SSSFTGRSLE VAE ESGIEGMLSRLEKERNVTFGAGFP             A+ETVA
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGAGFP------------GAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQGS+ISTPLSAKEGKWN+LGG L  NSSPVQINGLGG +SPEVQR+  W+T+ TEL+D
Sbjct: 241 DLQGSSISTPLSAKEGKWNNLGGSLSHNSSPVQINGLGGSVSPEVQRI--WNTYNTELRD 300

Query: 301 GDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
               ASE A   GR  EVLDV+RY TLGS  D   SN GEE+M+ +QIR +LQNLELELS
Sbjct: 301 ----ASENALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S+VGSLRSN  ASEKGQSRS+D+ LELSDA+EFQENEILNAKDKLRSIRAK+AVVEGKMA
Sbjct: 361 SMVGSLRSNTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           +TIIDAQKVIEEKQKR+NCARRALQ+LRTACVVWPNSASEV LVGSFDGW+TQRKMEKSS
Sbjct: 421 MTIIDAQKVIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           TGVFSLFLKLYPGKYEIKFIVDGQW+IDPLRPIVNSSGYENNLLIIT
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 509

BLAST of Lcy13g002470 vs. NCBI nr
Match: XP_022968253.1 (protein PTST homolog 2, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 828.2 bits (2138), Expect = 4.1e-236
Identity = 431/527 (81.78%), Postives = 465/527 (88.24%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           MLLFTNALPTSYF PT FGCLSPC FPS VFV  SRCG NS HMAATER C  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSGF+RRCKGLDSEG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSD RQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETVA 240
           STSSSFT RS ETVAE+ESGIEGML+RLE+ERN TFG       GKN LH ET A+ETVA
Sbjct: 181 STSSSFTSRSQETVAEHESGIEGMLNRLERERNATFG------TGKNGLHSETTAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKW +LG +L +NSSPVQING GG +S EVQ  RTWSTFKT+LQD
Sbjct: 241 DLQEIPISTPLSAKEGKWTNLGSYLSKNSSPVQINGQGGSVSSEVQ--RTWSTFKTDLQD 300

Query: 301 GDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ SE+A S+ RS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ Y+QNL+LELS
Sbjct: 301 GDFKGSEMALSEVRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRYIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S +GSLRSNA ASEKGQS+ SD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SAIGSLRSNAFASEKGQSKPSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LRTACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV+S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVSSDGYENNLLIVT 517

BLAST of Lcy13g002470 vs. NCBI nr
Match: KAG6574114.1 (Protein PTST-like 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 820.1 bits (2117), Expect = 1.1e-233
Identity = 427/527 (81.02%), Postives = 464/527 (88.05%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           MLLFTNALPTSYF P+ FGCLSP VFPSIVFV  SRCG NS HMAATER C  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPSPFGCLSPGVFPSIVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSGF+RRCK LD EG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSD RQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETVA 240
           STSSSFT RS ETV E+ESGIEGML+RLE+ERN TFG       GKN LHPET  +ETVA
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNATFG------TGKNCLHPETTVKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKWN+LG +L +NSSPVQING GG +S EVQ   TWSTFKT+L D
Sbjct: 241 DLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQ--GTWSTFKTDLPD 300

Query: 301 GDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ SE+A S+GRS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ ++QNL+LELS
Sbjct: 301 GDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           SV+GSLRSN+ ASEKGQS+SSD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LRTACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           TGVFSLFLKLYPGKYEIKF+VDGQWKIDPLRPIV S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFVVDGQWKIDPLRPIVRSDGYENNLLIVT 517

BLAST of Lcy13g002470 vs. NCBI nr
Match: XP_038892019.1 (protein PTST homolog 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 816.6 bits (2108), Expect = 1.2e-232
Identity = 427/527 (81.02%), Postives = 463/527 (87.86%), Query Frame = 0

Query: 1   MLLFTNALPTSY-FSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGM 60
           ML+FTNALPTSY F PT FG LSP V PSIVFV NS+ GWNS HMAATER C    SKGM
Sbjct: 1   MLVFTNALPTSYFFFPTDFGYLSPSVLPSIVFVPNSKWGWNSHHMAATERKCALSFSKGM 60

Query: 61  FYGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKE 120
            YGCSGF+ RCK LD EGDFALEAEIL+FM SSRNPEAFPSKKDLIEAGREDLVDAIVK+
Sbjct: 61  LYGCSGFVTRCKDLDREGDFALEAEILEFMRSSRNPEAFPSKKDLIEAGREDLVDAIVKK 120

Query: 121 GGWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIAS 180
           GGWLCLGW+LDEEEDG IG F L++R SILATDW++F  SNS K D  QRFAT +SPIAS
Sbjct: 121 GGWLCLGWNLDEEEDGGIGQFCLDERESILATDWDNFGLSNSLKCDESQRFATPLSPIAS 180

Query: 181 LSTSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETV 240
            STSSSFTGRSL+TVA++ESGIEGMLSRLEKERN TFGA      GKNDL PET A+ETV
Sbjct: 181 SSTSSSFTGRSLDTVAQDESGIEGMLSRLEKERNATFGA------GKNDLLPETTAKETV 240

Query: 241 ADLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQ 300
           ADLQGSTISTPLS K+ KW+DLG  L Q SSPVQ+NGLGG ++PEVQ  R+WSTFKT+LQ
Sbjct: 241 ADLQGSTISTPLSGKQDKWSDLGVCLSQGSSPVQLNGLGGSVTPEVQ--RSWSTFKTDLQ 300

Query: 301 DGDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELEL 360
           D DFEASEIA S GRS EVLDV RYE+LG R D TNSN  E++M+ NQ+R +LQN+ELEL
Sbjct: 301 DKDFEASEIALSTGRSGEVLDVLRYESLGYRGDSTNSNSSEKEMNHNQLRRHLQNMELEL 360

Query: 361 SSVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKM 420
           SSV+GSLRSNA  SEKGQS+SSD+LLELSDAFEFQENEILNAKDKLRSIRAKIAV+EGKM
Sbjct: 361 SSVIGSLRSNAFVSEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVIEGKM 420

Query: 421 ALTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKS 480
           ALTIIDAQKVIE KQKR+NCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKME+S
Sbjct: 421 ALTIIDAQKVIEGKQKRINCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMERS 480

Query: 481 STGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLII 527
           STGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVN+SGYENNLLII
Sbjct: 481 STGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNTSGYENNLLII 519

BLAST of Lcy13g002470 vs. NCBI nr
Match: XP_022151087.1 (uncharacterized protein LOC111019111 isoform X1 [Momordica charantia])

HSP 1 Score: 814.7 bits (2103), Expect = 4.7e-232
Identity = 418/527 (79.32%), Postives = 460/527 (87.29%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           ML+FTNALPT  F P+ FGCLSPCVFP++VFVSNSRCGWNS H AATER CV LSSKGMF
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSG +RRCKGLDSEGDFALEA+IL+FM+SS+NPEAFPSKKDLIEAGREDLVDAI K+G
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+LDEE D  +G +  EDR SI+ATDW+SF +SN+ + D R RFA A SP+AS 
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQETVA 240
           S SSSFTGRSLE VAE ESGIEGMLSRLEKERNVTFGAGFP             A+ETVA
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGAGFP------------GAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQGS+ISTPLSAKEGKWN+LGG L  NSSPVQINGLGG +SPEVQR+  W+T+ TEL+D
Sbjct: 241 DLQGSSISTPLSAKEGKWNNLGGSLSHNSSPVQINGLGGSVSPEVQRI--WNTYNTELRD 300

Query: 301 GDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDFEASE A   GR  EVLDV+RY TLGS  D   SN GEE+M+ +QIR +LQNLELELS
Sbjct: 301 GDFEASENALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S+VGSLRSN  ASEKGQSRS+D+ LELSDA+EFQENEILNAKDKLRSIRAK+AVVEGKMA
Sbjct: 361 SMVGSLRSNTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           +TIIDAQKVIEEKQKR+NCARRALQ+LRTACVVWPNSASEV LVGSFDGW+TQRKMEKSS
Sbjct: 421 MTIIDAQKVIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           TGVFSLFLKLYPGKYEIKFIVDGQW+IDPLRPIVNSSGYENNLLIIT
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 513

BLAST of Lcy13g002470 vs. NCBI nr
Match: XP_022968252.1 (protein PTST homolog 2, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 812.0 bits (2096), Expect = 3.0e-231
Identity = 431/558 (77.24%), Postives = 465/558 (83.33%), Query Frame = 0

Query: 1   MLLFTNALPTSYFSPTAFGCLSPCVFPSIVFVSNSRCGWNSRHMAATERSCVFLSSKGMF 60
           MLLFTNALPTSYF PT FGCLSPC FPS VFV  SRCG NS HMAATER C  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YGCSGF+RRCKGLDSEG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSD RQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRS-------------------------------LETVAENESGIEGMLSRLE 240
           STSSSFT RS                                ETVAE+ESGIEGML+RLE
Sbjct: 181 STSSSFTSRSQVFYSMPMKRNLIFYDNKMLCQDVKDTNFTNRETVAEHESGIEGMLNRLE 240

Query: 241 KERNVTFGAGFPAAVGKNDLHPETAAQETVADLQGSTISTPLSAKEGKWNDLGGHLGQNS 300
           +ERN TFG       GKN LH ET A+ETVADLQ   ISTPLSAKEGKW +LG +L +NS
Sbjct: 241 RERNATFG------TGKNGLHSETTAKETVADLQEIPISTPLSAKEGKWTNLGSYLSKNS 300

Query: 301 SPVQINGLGGLLSPEVQRLRTWSTFKTELQDGDFEASEIASSKGRSEEVLDVTRYETLGS 360
           SPVQING GG +S EVQ  RTWSTFKT+LQDGDF+ SE+A S+ RS EVLDV RYETLGS
Sbjct: 301 SPVQINGQGGSVSSEVQ--RTWSTFKTDLQDGDFKGSEMALSEVRSREVLDVLRYETLGS 360

Query: 361 RWDPTNSNFGEEKMDLNQIRSYLQNLELELSSVVGSLRSNALASEKGQSRSSDELLELSD 420
           R DPTNSN GEE+M  NQI+ Y+QNL+LELSS +GSLRSNA ASEKGQS+ SD+LLELSD
Sbjct: 361 RGDPTNSNSGEEEMSHNQIQRYIQNLQLELSSAIGSLRSNAFASEKGQSKPSDDLLELSD 420

Query: 421 AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARRALQLLRT 480
           AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKV+EEKQKR+NCARRALQ LRT
Sbjct: 421 AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRT 480

Query: 481 ACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP 528
           ACVVWPNSA+EVLLVGSFDGWSTQRKME+SSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP
Sbjct: 481 ACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP 540

BLAST of Lcy13g002470 vs. TAIR 10
Match: AT1G27070.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 346.3 bits (887), Expect = 4.4e-95
Identity = 216/474 (45.57%), Postives = 291/474 (61.39%), Query Frame = 0

Query: 63  CSGFLRRCK----GLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVK 122
           C+G  R C     G DSEG   LE EIL+FM++S  P  FPSKKDLI +GR DLV+ IV 
Sbjct: 72  CNGVSRMCNKRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVN 131

Query: 123 EGGWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDGRQRFATAVSPIA 182
           +GGWL +GW LDE+E+        + R +   T  +  ++      +  +   T      
Sbjct: 132 QGGWLSMGWDLDEQEE--------KVRVNENVTPQDLHIEKQLPNCNSPEMDKTLNH--G 191

Query: 183 SLSTSSSFTGRSLETVAENESGIEGMLSRLEKERNVTFGAGFPAAVGKNDLHPETAAQET 242
            L  SS+ +  + +  + N+SGIEG+L+RLEKERN++ G         N    + +   +
Sbjct: 192 DLDLSSNLSSSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGS 251

Query: 243 VADLQGSTISTPLSAKEGKWNDLGGHLGQN--SSPVQINGLGGLL-SPEVQRLRTWSTFK 302
           V     S I T    +E   +   G   Q+       ++G  GL  SP  +  RTWS  +
Sbjct: 252 VP--WSSRIVTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRR 311

Query: 303 TELQDGDFEASEIASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNL 362
               D DFEA+EI+SS G +    D T+ ++  S           E ++   I+  LQ L
Sbjct: 312 AGFTDEDFEAAEISSS-GLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQQL 371

Query: 363 ELELSSVVGSLRS--NALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIA 422
           + ELSSV+ SLRS  + + + K    ++  L  LSD +E++ENEI++A++KLRS RAK+A
Sbjct: 372 QSELSSVLHSLRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLA 431

Query: 423 VVEGKMALTIIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQ 482
           V+EGKMA+ IIDAQ+++ EKQ+R++ A RAL+LLRTA +VWPNSASEVLL GSFDGWSTQ
Sbjct: 432 VLEGKMAMAIIDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWSTQ 491

Query: 483 RKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 528
           RKM+K+  GVFSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLII+
Sbjct: 492 RKMKKAENGVFSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of Lcy13g002470 vs. TAIR 10
Match: AT5G03420.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 75.1 bits (183), Expect = 1.9e-13
Identity = 46/148 (31.08%), Postives = 75/148 (50.68%), Query Frame = 0

Query: 383 ELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARR 442
           E+  L    + +E E+   K+++   +  ++V++ +    I  AQ +I EK+  +  A  
Sbjct: 446 EIDRLRFMLDQKELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEE 505

Query: 443 ALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMEKSST------GVFSLFLKLYPGKYE 502
           +L  L+   + +    + V + GSF+GW  +  ME  ++        +S  L LYPG YE
Sbjct: 506 SLSGLQEVVIEYCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYE 565

Query: 503 IKFIVDGQWKIDPLRPIVNSSGYENNLL 525
           IKFIVDGQW  DP +  V      NN+L
Sbjct: 566 IKFIVDGQWITDPQKDSVTRGHISNNIL 593

BLAST of Lcy13g002470 vs. TAIR 10
Match: AT5G39790.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 68.9 bits (167), Expect = 1.4e-11
Identity = 53/191 (27.75%), Postives = 90/191 (47.12%), Query Frame = 0

Query: 346 NQIRSYLQNLELELSSVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKL 405
           ++++S L  ++ E S +V  L S A    +   R   E+  +       E E+  A  KL
Sbjct: 87  DELKSLL--IDTERSKLVKKL-SEANQQNRFLKRQEHEITNIKTELALMELEV-QALVKL 146

Query: 406 RSIRAKIAVVEGKMALT-------IIDAQKVIEEKQKRMNCARRALQLLRTACVVWPNSA 465
               A + + +G   ++       ++     +++K K       A Q  +   V W   A
Sbjct: 147 AEEIANLGIPQGSRKISGKYIQSHLLSRLDAVQKKMKEQIKGVEAAQ-SKEVHVFWIGMA 206

Query: 466 SEVLLVGSFDGWSTQRKMEKSSTGVFSLF---LKLYPGKYEIKFIVDGQWKIDPLRPIVN 525
             V ++GSFDGWS +  +    + +F+ F   L L PG+YE+KF+VDG+W+I P  P   
Sbjct: 207 ESVQVMGSFDGWSQREDLSPEYSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSG 266

Query: 526 SSGYENNLLII 527
               ENN+L++
Sbjct: 267 EGLMENNVLVV 272

BLAST of Lcy13g002470 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 54.7 bits (130), Expect = 2.6e-07
Identity = 25/74 (33.78%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 452 VVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 511
           + W +   E+ + GS+D W T+ ++++S    F++   L  G YE +FIVDGQW+  P  
Sbjct: 76  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 135

Query: 512 PIV-NSSGYENNLL 525
           P+  + +G   N+L
Sbjct: 136 PLARDDAGNTFNIL 148

BLAST of Lcy13g002470 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 54.7 bits (130), Expect = 2.6e-07
Identity = 25/74 (33.78%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 452 VVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 511
           + W +   E+ + GS+D W T+ ++++S    F++   L  G YE +FIVDGQW+  P  
Sbjct: 78  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 137

Query: 512 PIV-NSSGYENNLL 525
           P+  + +G   N+L
Sbjct: 138 PLARDDAGNTFNIL 150