Lcy12g019400 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g019400
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Isoaspartyl peptidase/L-asparaginase
Location	Chr12: 46753965 .. 46755617 (-)
RNA-Seq Expression	Lcy12g019400
Synteny	Lcy12g019400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTGAACAAGGCAGAGAGAGGAGAGATGGGAGGTTGGGCCATAGCGCTTCATGGTGGGGCTGGCGTGGATCCTAATCTCCCACTGCATCGTCAAGAAGATGCCAAAAATCTTCTCACTCGCTGCCTTGATCTTGGCATCCATGCTCTTCGTTCGAACCACTCGGCCATTGATGTCGTCGAACTCGTCGTACGCCACCCCCCCCTTTATCTATTTCTCTTCTCATTCTTTCCCTTCATATAATTTGACATGTTTATACTTATAATCATGACCCATTACGAATTTTCAAGGTTAGAGAACTGGAAACTGATCCCCTGTTCAATTCCGGGCGTGGATCTGCGCTGACAGAGGATGGGACTGTAGAGATGGAGGCCAGCATCATGGACGGGCCAAAACGGAGATGCGGGGCGGTTTCTGGACTCACAACCGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCTCCCCATTCCTATCTGGCCTTCTCCGGCGCTGAGAAATTCGCTAAGCAACAGGTTCGTTTTCCTTATTCTATTCATTCCCCCCTTTTTTTTCCCGATTCGTAAGGTCGGCTTTTCTGACTCTTTGGAACTGGAATTGTATTAGGGTGTGGAGCTTGTGTGCAATGACTATTTCATCACCGACGAAAACGTCGGAATGCTTGAACAAGCCAAAGAAGCCAACAGTATTTGGGTACGCCCGCAATTCAAATATGTTAATCAAATATAATTCAAAATAACTGTTCGAAGCGGACTGATATTTTTGGTTTTGATTTGGAACAGTTGGATTATAGGGTCCCAAACGAGACGTGTAGCGCTAGCGTGGAGACACCGTTGCAAATGAACGGCCTCCCGATAAGCGTGTACGCGCCGGAGACGGTGGGTTGCGTGGTGGTTGACAGCGAAGGAAGGTGCGCCGCCGCCACGTCCACCGGCGGCCTGATTAACAAAAAGATCGGCCGTATCGGAGACTCGCCGCTGATTGGGTCGGGGACGTACGCGTGCGGTGTGTGCGGGGTTTCGTGCACCGGAGAAGGGGAGGCGATCATAAGAGGAACTCTGGCTAGAGAAGTGGCGGCGGTGATGGAGTACAAGGGATTGGGACTTCAAGAGGCAGTGAATTACGTGATAGAAGAGAGGCTGGACGAAGGCAAGGCCGGACTGATTGCAGTGTCCAGCAATGGAGAAGTGGCGTGTGGGTTCAACACCACCGGGATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGAATTTGGAAATGAACTGAATAGTTAGATAAATTAAGTTTGGAATCAAGTTTTATGTGTGGGTTTTAATATTTTTAGTGGTGTGTTACTGAAACTGTGTCAATCTCTTCTTCTGGATTTTTCTGAACTCGATCTGTTAGCAAATAAAAGAGCCATTAGCCCTAACTTTGTTAATTATAAAGTTGTTCTAAGTTTTCTATTCAGTGTCTGACAATTAAGCTGTTCTAAATTTTATTTTCAGTGTATGACAAAGTTGTTCTAAGTTTTCTTTTCAGTGTCCGACAATTAAGTTGTTCTAAGTTTTCTTTTCAGTGTCCGACGATTAAGTTGTTCTAAGTTTTCTTTTCAGTGTCCGACAATTAAGTTGTTCTAAGTTTTCTTTTCAGTGTC

mRNA sequence

AGTGAACAAGGCAGAGAGAGGAGAGATGGGAGGTTGGGCCATAGCGCTTCATGGTGGGGCTGGCGTGGATCCTAATCTCCCACTGCATCGTCAAGAAGATGCCAAAAATCTTCTCACTCGCTGCCTTGATCTTGGCATCCATGCTCTTCGTTCGAACCACTCGGCCATTGATGTCGTCGAACTCGTCGTTAGAGAACTGGAAACTGATCCCCTGTTCAATTCCGGGCGTGGATCTGCGCTGACAGAGGATGGGACTGTAGAGATGGAGGCCAGCATCATGGACGGGCCAAAACGGAGATGCGGGGCGGTTTCTGGACTCACAACCGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCTCCCCATTCCTATCTGGCCTTCTCCGGCGCTGAGAAATTCGCTAAGCAACAGGGTGTGGAGCTTGTGTGCAATGACTATTTCATCACCGACGAAAACGTCGGAATGCTTGAACAAGCCAAAGAAGCCAACAGTATTTGGTTGGATTATAGGGTCCCAAACGAGACGTGTAGCGCTAGCGTGGAGACACCGTTGCAAATGAACGGCCTCCCGATAAGCGTGTACGCGCCGGAGACGGTGGGTTGCGTGGTGGTTGACAGCGAAGGAAGGTGCGCCGCCGCCACGTCCACCGGCGGCCTGATTAACAAAAAGATCGGCCGTATCGGAGACTCGCCGCTGATTGGGTCGGGGACGTACGCGTGCGGTGTGTGCGGGGTTTCGTGCACCGGAGAAGGGGAGGCGATCATAAGAGGAACTCTGGCTAGAGAAGTGGCGGCGGTGATGGAGTACAAGGGATTGGGACTTCAAGAGGCAGTGAATTACGTGATAGAAGAGAGGCTGGACGAAGGCAAGGCCGGACTGATTGCAGTGTCCAGCAATGGAGAAGTGGCGTGTGGGTTCAACACCACCGGGATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGAATTTGGAAATGAACTGAATAGTTAGATAAATTAAGTTTGGAATCAAGTTTTATGTGTGGGTTTTAATATTTTTAGTGGTGTGTTACTGAAACTGTGTCAATCTCTTCTTCTGGATTTTTCTGAACTCGATCTGTTAGCAAATAAAAGAGCCATTAGCCCTAACTTTGTTAATTATAAAGTTGTTCTAAGTTTTCTATTCAGTGTCTGACAATTAAGCTGTTCTAAATTTTATTTTCAGTGTATGACAAAGTTGTTCTAAGTTTTCTTTTCAGTGTCCGACAATTAAGTTGTTCTAAGTTTTCTTTTCAGTGTCCGACGATTAAGTTGTTCTAAGTTTTCTTTTCAGTGTCCGACAATTAAGTTGTTCTAAGTTTTCTTTTCAGTGTC

Coding sequence (CDS)

ATGGGAGGTTGGGCCATAGCGCTTCATGGTGGGGCTGGCGTGGATCCTAATCTCCCACTGCATCGTCAAGAAGATGCCAAAAATCTTCTCACTCGCTGCCTTGATCTTGGCATCCATGCTCTTCGTTCGAACCACTCGGCCATTGATGTCGTCGAACTCGTCGTTAGAGAACTGGAAACTGATCCCCTGTTCAATTCCGGGCGTGGATCTGCGCTGACAGAGGATGGGACTGTAGAGATGGAGGCCAGCATCATGGACGGGCCAAAACGGAGATGCGGGGCGGTTTCTGGACTCACAACCGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCTCCCCATTCCTATCTGGCCTTCTCCGGCGCTGAGAAATTCGCTAAGCAACAGGGTGTGGAGCTTGTGTGCAATGACTATTTCATCACCGACGAAAACGTCGGAATGCTTGAACAAGCCAAAGAAGCCAACAGTATTTGGTTGGATTATAGGGTCCCAAACGAGACGTGTAGCGCTAGCGTGGAGACACCGTTGCAAATGAACGGCCTCCCGATAAGCGTGTACGCGCCGGAGACGGTGGGTTGCGTGGTGGTTGACAGCGAAGGAAGGTGCGCCGCCGCCACGTCCACCGGCGGCCTGATTAACAAAAAGATCGGCCGTATCGGAGACTCGCCGCTGATTGGGTCGGGGACGTACGCGTGCGGTGTGTGCGGGGTTTCGTGCACCGGAGAAGGGGAGGCGATCATAAGAGGAACTCTGGCTAGAGAAGTGGCGGCGGTGATGGAGTACAAGGGATTGGGACTTCAAGAGGCAGTGAATTACGTGATAGAAGAGAGGCTGGACGAAGGCAAGGCCGGACTGATTGCAGTGTCCAGCAATGGAGAAGTGGCGTGTGGGTTCAACACCACCGGGATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGAATTTGGAAATGA

Protein sequence

MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELETDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEDGFMEVGIWK

Homology

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BLAST of Lcy12g019400 vs. ExPASy Swiss-Prot
Match: Q8GXG1 (Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g16150 PE=2 SV=2)

HSP 1 Score: 531.6 bits (1368), Expect = 6.3e-150
Identity = 261/325 (80.31%), Postives = 292/325 (89.85%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAG+DPNLP  RQE+AK LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETC--SASVETP 180
           AFSGAE FA++QGVE+V N+YF+TD+NVGML+ AKEANSI  DYR+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGL+NK +GRIGDSPLIG+GTYA   C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIWK 324
           CGFN+ GMFRGCATEDGFMEV IW+
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIWE 325

[Close]

BLAST of Lcy12g019400 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 372.1 bits (954), Expect = 6.4e-102
Identity = 194/323 (60.06%), Postives = 233/323 (72.14%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDP-NLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIALHGGAG  P +LP  R+   ++ L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
             P FN+G+GS LT  GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPL 180
           LAF  AE FA+  GVE V + +FIT EN+  L+QAKE N + LDY VP      S + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVP------SPKVPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of Lcy12g019400 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.5e-100
Identity = 196/327 (59.94%), Postives = 234/327 (71.56%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDP-NLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IALHGGAG  P +LP  R++  +  L  CL +G+ AL+S    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPN----ETCSASV 180
           LAF GA+ FAKQQGVE V + +FIT ENV  L+ A EAN + +DY   N        A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GEAII  T+AR+VAA+ME+KGL L+EA +YV+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEDGFMEVGIW 323
           E+A  FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRASATEDGYSEIAIW 322

[Close]

BLAST of Lcy12g019400 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.0e-99
Identity = 195/327 (59.63%), Postives = 234/327 (71.56%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDP-NLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IALHGGAG  P +LP  R++  +  L  CL +G+ AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPN----ETCSASV 180
           LAF GA+ FAKQQGVE V + + IT ENV  L+ A EAN + +DY   N        A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLTNGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GE IIR T+AR+VAA+ME+KGL L+EA ++VI ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEDGFMEVGIW 323
           E+A  FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of Lcy12g019400 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.8e-99
Identity = 194/327 (59.33%), Postives = 234/327 (71.56%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDP-NLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IALHGGAG  P +LP  R++  +  L  CL +GI AL++ +  +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL TV NPISLAR VMDK+PH +
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIF 120

Query: 121 LAFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNET----CSASV 180
           LAF GA+   KQQGVE V + +FIT+ENV  L+ A EAN + +DY   N T      A  
Sbjct: 121 LAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GEAIIR T+AR+VAA+ME+KGL L+EA + V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEDGFMEVGIW 323
           E+A  FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of Lcy12g019400 vs. ExPASy TrEMBL
Match: A0A6J1IYV7 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481149 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 308/323 (95.36%), Postives = 316/323 (97.83%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPL RQEDAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA++QGVEL CNDYF+T+ENVGMLE A+EANSIWLDYRVPNE CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGL+NKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of Lcy12g019400 vs. ExPASy TrEMBL
Match: A0A6J1FND2 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446743 PE=4 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 7.7e-175
Identity = 306/323 (94.74%), Postives = 315/323 (97.52%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPL RQEDAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA++QGVEL CNDYF+T+ENVGMLE A+EANSIWLDYRVPNE CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of Lcy12g019400 vs. ExPASy TrEMBL
Match: A0A5D3D9K2 (Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003020 PE=4 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.0e-166
Identity = 296/322 (91.93%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPLHRQ+DAK  LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA+QQGVELV NDYFIT+ NV +L+ AKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of Lcy12g019400 vs. ExPASy TrEMBL
Match: A0A1S3BV13 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC103493576 PE=4 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.0e-166
Identity = 296/322 (91.93%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPLHRQ+DAK  LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA+QQGVELV NDYFIT+ NV +L+ AKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of Lcy12g019400 vs. ExPASy TrEMBL
Match: A0A6J1CHT1 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 GN=LOC111011731 PE=4 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.6e-165
Identity = 291/325 (89.54%), Postives = 310/325 (95.38%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           M GWAIA+HGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+H AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPN---ETCSASVET 180
           AFSGAE+FA+QQGVE+VCNDYFIT+ENVGML+ AKEANSI  DYR+PN   E CSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGV 240
           PLQMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEDGFMEVGIW 323
           ACGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

[Close]

BLAST of Lcy12g019400 vs. NCBI nr
Match: XP_022982271.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima])

HSP 1 Score: 625.5 bits (1612), Expect = 2.5e-175
Identity = 308/323 (95.36%), Postives = 316/323 (97.83%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPL RQEDAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA++QGVEL CNDYF+T+ENVGMLE A+EANSIWLDYRVPNE CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGL+NKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of Lcy12g019400 vs. NCBI nr
Match: XP_022941449.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037783.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 306/323 (94.74%), Postives = 315/323 (97.52%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPL RQEDAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA++QGVEL CNDYF+T+ENVGMLE A+EANSIWLDYRVPNE CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of Lcy12g019400 vs. NCBI nr
Match: XP_023525179.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620.5 bits (1599), Expect = 7.9e-174
Identity = 307/324 (94.75%), Postives = 316/324 (97.53%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPL RQEDAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNSSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVP-NETCSASVETPL 180
           AFSGAEKFA++QGVEL CNDYF+T+ENVGMLE A+EANSIWLDYRVP NE CSASVETPL
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPTNEKCSASVETPL 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCG 240
           QMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIGSGTYACGVCG
Sbjct: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV C
Sbjct: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIWK 324
           GFNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 GFNTTGMFRGCATEDGFMEVGIWK 324

[Close]

BLAST of Lcy12g019400 vs. NCBI nr
Match: XP_038896168.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida])

HSP 1 Score: 617.5 bits (1591), Expect = 6.7e-173
Identity = 304/322 (94.41%), Postives = 315/322 (97.83%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPLHRQ DAK LLTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQHDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA+QQGVELV NDYFIT++NVG+LE AKEANSIWLD+RVP+ETCSASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVGNDYFITEDNVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGL ISVYAPETVGCVVVD+EGRCAAATSTGGLINKK+GRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLRISVYAPETVGCVVVDTEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of Lcy12g019400 vs. NCBI nr
Match: XP_008452604.1 (PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA0064417.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa] >TYK20170.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 595.9 bits (1535), Expect = 2.1e-166
Identity = 296/322 (91.93%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAGVDPNLPLHRQ+DAK  LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQ 180
           AFSGAEKFA+QQGVELV NDYFIT+ NV +L+ AKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of Lcy12g019400 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 531.6 bits (1368), Expect = 4.5e-151
Identity = 261/325 (80.31%), Postives = 292/325 (89.85%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDPNLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIA+HGGAG+DPNLP  RQE+AK LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETC--SASVETP 180
           AFSGAE FA++QGVE+V N+YF+TD+NVGML+ AKEANSI  DYR+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGL+NK +GRIGDSPLIG+GTYA   C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIWK 324
           CGFN+ GMFRGCATEDGFMEV IW+
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIWE 325

[Close]

BLAST of Lcy12g019400 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.6e-103
Identity = 194/323 (60.06%), Postives = 233/323 (72.14%), Query Frame = 0


Query: 1   MGGWAIALHGGAGVDP-NLPLHRQEDAKNLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIALHGGAG  P +LP  R+   ++ L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
             P FN+G+GS LT  GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFAKQQGVELVCNDYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPL 180
           LAF  AE FA+  GVE V + +FIT EN+  L+QAKE N + LDY VP      S + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVP------SPKVPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of Lcy12g019400 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 285.4 bits (729), Expect = 5.6e-77
Identity = 146/243 (60.08%), Postives = 177/243 (72.84%), Query Frame = 0


Query: 80  MEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFAKQQGVELVCN 139
           MEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH YLAF  AE FA+  GVE V +
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 DYFITDENVGMLEQAKEANSIWLDYRVPNETCSASVETPLQMNGLPISVYAPETVGCVVV 199
            +FIT EN+  L+QAKE N + LDY VP      S + P       I      TVGCV V
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVP------SPKVPDNCGDSQIG-----TVGCVAV 120

Query: 200 DSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACGVCGVSCTGEGEAIIRGTLAREVA 259
           DS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C +S TG+GE IIRGT+AR+VA
Sbjct: 121 DSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVA 180

Query: 260 AVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEDGFMEV 319
           A+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV   FNTTGMFR CA+EDG+ E+
Sbjct: 181 ALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEI 232

Query: 320 GIW 323
            IW
Sbjct: 241 AIW 232

[Close]

BLAST of Lcy12g019400 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 6.1e-23
Identity = 92/336 (27.38%), Postives = 145/336 (43.15%), Query Frame = 0


Query: 6   IALHGGAGVDPNLPLHRQEDAKNLLTR-CLDLGIHALRSNHSAIDVVELVVRELETDPLF 65
           +A+H GAG         ++  ++++ R CL       + +   ID V   ++ LE DP  
Sbjct: 15  VAVHVGAGYH---AAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 66  NSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARL----------VMDK 125
           N+GRGS LTEDG VE +AS+MDG     G V  +  V+N I +A L          ++ +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 126 SPHSYLAFSGAEKFAKQQ-----GVELVCNDYFITDENVGMLEQAKE------ANSIWLD 185
            P   L   GA ++ K +     G     + + +T+       + K       A SI   
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 186 YRVP-----NETCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKK 245
              P     NETC  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K 
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKI 254

Query: 246 IGRIGDSPLIGSGTYACG--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQ 298
            GR+G +   GSG +A          + G   +G GE ++RG  ARE    +     G  
Sbjct: 255 SGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPA 314

[Close]

BLAST of Lcy12g019400 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-19
Identity = 90/328 (27.44%), Postives = 144/328 (43.90%), Query Frame = 0


Query: 6   IALHGGAGVDPNLPLHRQEDAKNLLTR-CLDLGIHALRSNHSAIDVVELVVRELETDPLF 65
           +A+H GAG         ++  ++++ R CL       + +   ID V   ++ LE DP  
Sbjct: 15  VAVHVGAGYH---AAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 66  NSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPIS--LARLVMDKSPHSYLAF 125
           N+GRGS LTEDG VE +AS+MDG     G   G+  V + +S  L++L++       L  
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDS---GMFGGVGAVPDQVSEMLSKLLL----CWLLVG 134

Query: 126 SGAEKFAKQQ-----GVELVCNDYFITDENVGMLEQAKE------ANSIWLDYRVP---- 185
            GA ++ K +     G     + + +T+       + K       A SI      P    
Sbjct: 135 EGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTE 194

Query: 186 -NETCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSP 245
            NETC  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K  GR+G + 
Sbjct: 195 NNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAA 254

Query: 246 LIGSGTYACG--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIE 298
             GSG +A          + G   +G GE ++RG  ARE    +     G   A   V+ 
Sbjct: 255 TYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLR 314

The following BLAST results are available for this feature:

BLAST of Lcy12g019400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GXG1	6.3e-150	80.31	Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 ...	[more]
P50287	6.4e-102	60.06	Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08...	[more]
P30364	3.5e-100	59.94	Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1	[more]
Q9ZSD6	1.0e-99	59.63	Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1	[more]
P50288	1.8e-99	59.33	Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1	[more]

BLAST of Lcy12g019400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IYV7	1.2e-175	95.36	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FND2	7.7e-175	94.74	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5D3D9K2	1.0e-166	91.93	Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BV13	1.0e-166	91.93	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1CHT1	5.6e-165	89.54	probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Lcy12g019400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982271.1	2.5e-175	95.36	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima]	[more]
XP_022941449.1	1.6e-174	94.74	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447...	[more]
XP_023525179.1	7.9e-174	94.75	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo]	[more]
XP_038896168.1	6.7e-173	94.41	probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida]	[more]
XP_008452604.1	2.1e-166	91.93	PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA00...	[more]

BLAST of Lcy12g019400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16150.1	4.5e-151	80.31	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.1	4.6e-103	60.06	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.2	5.6e-77	60.08	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.1	6.1e-23	27.38	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.2	1.1e-19	27.44	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.60.20.30	(Glycosyl)asparaginase	coord: 193..323 e-value: 6.1E-43 score: 148.1
None	No IPR available	PIRSR	PIRSR600246-2	PIRSR600246-2	coord: 3..319 e-value: 2.5E-92 score: 307.2
None	No IPR available	PIRSR	PIRSR600246-1	PIRSR600246-1	coord: 3..319 e-value: 2.5E-92 score: 307.2
None	No IPR available	PIRSR	PIRSR600246-3	PIRSR600246-3	coord: 3..319 e-value: 2.5E-92 score: 307.2
None	No IPR available	PANTHER	PTHR10188:SF36	PEPTIDASE T2, ASPARAGINASE 2, NUCLEOPHILE AMINOHYDROLASE-RELATED	coord: 1..322
None	No IPR available	CDD	cd04701	Asparaginase_2	coord: 5..315 e-value: 4.56615E-123 score: 351.764
IPR000246	Peptidase T2, asparaginase 2	PFAM	PF01112	Asparaginase_2	coord: 4..322 e-value: 7.1E-103 score: 343.9
IPR000246	Peptidase T2, asparaginase 2	PANTHER	PTHR10188	L-ASPARAGINASE	coord: 1..322
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 25..321

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g019400.1	Lcy12g019400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016540	protein autoprocessing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004067	asparaginase activity
molecular_function	GO:0008798	beta-aspartyl-peptidase activity
molecular_function	GO:0016787	hydrolase activity