Lcy12g019250 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g019250
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Cytochrome P450
Location	Chr12: 46672880 .. 46676568 (-)
RNA-Seq Expression	Lcy12g019250
Synteny	Lcy12g019250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGCAAAGTTTCGAAGGATGCAGTGTTGGGACGAAGAGTGAGTGATAGTAGTGAAGCCATGGGAGTTTTAGGAGCTATATTTTTATACATTCCACTGTTTTTAGTGTTGTACACATTAACAGAGCAGTTTCTTCACAAAATTCGAAACCTCCCGCCGACCCCTTTCCCGTCGGTTCCAGTCATCGGCCATCTCCACCTTCTGAAGAAGCCCCTCTACCGGACTTTGGCGAAGATCTCGAACCGGTACGGCCCGGTTGTCTTTCTCCGGTTCGGATCACGCCAAGTTCTCGTGGTGTCGTCTCCTTCCGCGGCCGAAGAATGTCTGACCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTCCTCCTATCCAAGTGGTTCGGATACAACAACACGAACCTCGTCTGGTCATCGTATGGGGACCACTGGCGCAACCTCCGCCGAATCTCCACCGTTGAAGTCTTGTCCACTCATCGCCTTCAGATGCTCTCGACCGTCCGTCTGGAAGAGGTCAACTCTTTGATTCGGAGGCTCGCTAGAAGCGAAACCCAGGTGCATAACATGAAGAATGCGTTTTTCGATCTGACTTATAACATCATATTGAGGATGATTGTCGGAAAGCGGTTTTACGGTGATGACGTGGACGATGCTGACGAGGCAAGATGTTATAGAGAGTTACAGGCTGAGTCAACTCGATTGAGTGGTAAGTCAAATATAGGAGATTTTATCCCGTTGATGTCGTGGCTGGGATTTGGATCGGCTGTGGAGAATGAGATAATGGAATGTCACAAAAGAAGAGACGCTTTCATGCAGAGTTTGATTGACCAGCAAAGGAAAAGAATGGTTCAAACTGAAACTGGAGATTCCTTTCGCGATGGAAGGAAGAAAACGATGATTGAAGTACTGTTAGAGCTACAAGAATCTCAACCTGAGCAGTACAATGACGAAACTATCAAAGCCTTGATGCTGGTAATCATTTTTCTTCTTTATTTAGTGTTCAATTTAACCTTGATTGCTTTGGTTGTGTTATTCAATTAGCCCACATATGCAAAATTCAAAATTTGAGAATATATTTAAACCAAGATTCAAAATTGACGTTGGACATCAAATTAAAAGTTCAAAAACTCGCTAGATAGTTTATGAGTATGTTTATGTTCAATCACACACACTATATATAACATGGTTTTTTAATCAAGTCTACCTCATATATTAAAAATAGTCTCTACATATTTTATTCAAGGATATGCATGAATCACAATTTGAATTTATGACATTTTCATTGTTTTTTTTTTTTTTTTTTTGTACACCATCTTTAAATAATTGATTTTTTATTATTATTTTAGTTCAAATAATTTAAAGTAGGACATATTAGAGACTTCTTAAAATATAGGTAAATGTTTATAATGCATGTGATTTTCTTTATTTTCTTTATCCTATATAAAAAATTTGAATATCTCACTTTTTAATCAACAATACATGTTTTATGTGAGTTGAGTTGTACTTGTGTTCGTCATGTGATTTAGTTTAAGATTCAATAGTTAGTCTTTATTTAATTATTTTAGTTTCCGTTGATCACATAAGTTCTGGTGTCACCCAACTTAGATTAAAGCATTGATTCGGGGAAAAGACAAAAGCAATTAAAATAATACGAAATTATGAAGATGTGAATGTGATTGGTGATAAGTTTCAATGGTTAATTTGTCTCGTAGAGGCCACTCCGGGTTTAAATTATATAACTAATATTTGGGTGACTTCAAAGTTTTGTCGTGAATGGAGATGAGTGACTCCGAAAAAGAAAAAAGAAAAATATTTTTATTTTCAGTACAGAAGAAAAATGTGTTTTTTTTAAGAAGAAAATGTTATTTTATACGACTATATCACCGATTTTTTTACATGAGTGGTCGAACTTTACATGGTATCCAGTTGTAAAGCATTTATTATTATTATTATTATTTTGTCTAGATAAGTATATTGATTTGATATGCATTTGAACTTTTTTTTTTAGAAAAAATGCTATGTATTTGAATAACTATTCATTCTTTTCAAAAAGAAAATGAAATTAAATATCCATCCATCTGCGGTGGATAAGACACCCATTAAAATTATAAACTAACTAATCTCTCCTATTAAAAAGCTTAAAATCTTTCAGCTTTGGCCACGTAGTCAACTTATTCCAATAATTTATTTCTATCTTTAAAAAGCTTAAACTATTATTATTATTAAAAAAAAAAAACTATTATTATTTTAAAAAGTTAGAATTACATATTTAATCTCTAAACTTTTAGGTTTGTGACTATTTAGTACTTAAACTTTTAAAAGTATTTAATAAGTTTCTCAACTTTCATTTTGTTTATTTGGTTTAAAAACTTTAAAAAGTATCTAATAAATTATTAAACTTTCAACTTTGTGTCGAATAAGTCTTTGAATTTCCAATTTTATGTCTAAAAGTCATTGAGCTATTTGATGTTTTAATTTTAAAATTCATGTATCTACTAGACACAAAATTGAAAATTTTTAAGGTTCTATGGAACATAAATACAAAATTAAAAATTCAGAGATTTATTAGATATTTTTAGAGTTTGTAACCCTCATAAAATTGAAAGTCTAGAAACTAGAAACTCTTTAAATGTAGGTATCAAACAAACACGGACTTATAAATTCAAATATTACACTTTATTTAAACTGTTTTTAAAGCTATGTACTGTATATGTATTTATATTGTTTATTTATTTATTCGAAATAATGTATTTATAGTGTTTATATTAATTCCGTAGCTGTTTGAATATTTAATATTTAGTGTGTACGTGGACAGGTTCTGTTGGCTGCAGGTACGGAAACATCAGTGAACACGATGGAATGGGCCCTATCTCTTATGTTGAACCAACCAGAAGTTTTAAAGAAAGCGCAGGTCGAAATTGACTGTCAAGTTGGCCAAGACCGTCTGATTGACGAATCTGACGTGGCGAACCTTCCCTACCTACGTGGCATCATCAACGAAACCTTACGAATGTACCCTCCAGGTCCACTGATGGTACCCCACGAGTCATCAAAAGACTGTAGCGTTGGAGGATACCATATCCCACGTGGCACTATGCTGTACGTGAATTTATGGGCGATTCATAACGACCCCAAGAATTGGGGTGAGCCGAGAAAGTTTGATCCCGAGAGGTTTGAGAGGGTGGAAAGTGAGAAGTATGGGTTCAATCTGATGCCGTTCGGGGCGGGTAGAAGGGGCTGTCCAGGAGAGGGATTGGGGCTTCGGGTGATTGGGCTTGTAATGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGCGAGGAGTTGGTGGATATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACGCTTGCTCGCTAAATGTACGCCTCGACCCATTGTCGAACATCTTGATCTTTCTCCAACCAGATCCAACCCGGCCCATTATAAAAATTGAAAACATTAATTTATAACCATTACTATTAGGGGCCGTTTGGTACAAGGTTTTTGGAGATGAGAATGGGCAAGACTTTCTAATACCCTTGTTTGTTTCAAGATTTATGTGGTGGGTCCAGGAAATAACGCATTCCCAGACATCATTTATGCCTATAAGCAGCCTTTCTATGCCTTTGCAAAAGCATGAGTTTTCTCTTTTCCCATCTCTTCTTTTTTATTTTTATAT

mRNA sequence

CAGCAAAGTTTCGAAGGATGCAGTGTTGGGACGAAGAGTGAGTGATAGTAGTGAAGCCATGGGAGTTTTAGGAGCTATATTTTTATACATTCCACTGTTTTTAGTGTTGTACACATTAACAGAGCAGTTTCTTCACAAAATTCGAAACCTCCCGCCGACCCCTTTCCCGTCGGTTCCAGTCATCGGCCATCTCCACCTTCTGAAGAAGCCCCTCTACCGGACTTTGGCGAAGATCTCGAACCGGTACGGCCCGGTTGTCTTTCTCCGGTTCGGATCACGCCAAGTTCTCGTGGTGTCGTCTCCTTCCGCGGCCGAAGAATGTCTGACCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTCCTCCTATCCAAGTGGTTCGGATACAACAACACGAACCTCGTCTGGTCATCGTATGGGGACCACTGGCGCAACCTCCGCCGAATCTCCACCGTTGAAGTCTTGTCCACTCATCGCCTTCAGATGCTCTCGACCGTCCGTCTGGAAGAGGTCAACTCTTTGATTCGGAGGCTCGCTAGAAGCGAAACCCAGGTGCATAACATGAAGAATGCGTTTTTCGATCTGACTTATAACATCATATTGAGGATGATTGTCGGAAAGCGGTTTTACGGTGATGACGTGGACGATGCTGACGAGGCAAGATGTTATAGAGAGTTACAGGCTGAGTCAACTCGATTGAGTGGTAAGTCAAATATAGGAGATTTTATCCCGTTGATGTCGTGGCTGGGATTTGGATCGGCTGTGGAGAATGAGATAATGGAATGTCACAAAAGAAGAGACGCTTTCATGCAGAGTTTGATTGACCAGCAAAGGAAAAGAATGGTTCAAACTGAAACTGGAGATTCCTTTCGCGATGGAAGGAAGAAAACGATGATTGAAGTACTGTTAGAGCTACAAGAATCTCAACCTGAGCAGTACAATGACGAAACTATCAAAGCCTTGATGCTGGTTCTGTTGGCTGCAGGTACGGAAACATCAGTGAACACGATGGAATGGGCCCTATCTCTTATGTTGAACCAACCAGAAGTTTTAAAGAAAGCGCAGGTCGAAATTGACTGTCAAGTTGGCCAAGACCGTCTGATTGACGAATCTGACGTGGCGAACCTTCCCTACCTACGTGGCATCATCAACGAAACCTTACGAATGTACCCTCCAGGTCCACTGATGGTACCCCACGAGTCATCAAAAGACTGTAGCGTTGGAGGATACCATATCCCACGTGGCACTATGCTGTACGTGAATTTATGGGCGATTCATAACGACCCCAAGAATTGGGGTGAGCCGAGAAAGTTTGATCCCGAGAGGTTTGAGAGGGTGGAAAGTGAGAAGTATGGGTTCAATCTGATGCCGTTCGGGGCGGGTAGAAGGGGCTGTCCAGGAGAGGGATTGGGGCTTCGGGTGATTGGGCTTGTAATGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGCGAGGAGTTGGTGGATATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACGCTTGCTCGCTAAATGTACGCCTCGACCCATTGTCGAACATCTTGATCTTTCTCCAACCAGATCCAACCCGGCCCATTATAAAAATTGAAAACATTAATTTATAACCATTACTATTAGGGGCCGTTTGGTACAAGGTTTTTGGAGATGAGAATGGGCAAGACTTTCTAATACCCTTGTTTGTTTCAAGATTTATGTGGTGGGTCCAGGAAATAACGCATTCCCAGACATCATTTATGCCTATAAGCAGCCTTTCTATGCCTTTGCAAAAGCATGAGTTTTCTCTTTTCCCATCTCTTCTTTTTTATTTTTATAT

Coding sequence (CDS)

ATGGGAGTTTTAGGAGCTATATTTTTATACATTCCACTGTTTTTAGTGTTGTACACATTAACAGAGCAGTTTCTTCACAAAATTCGAAACCTCCCGCCGACCCCTTTCCCGTCGGTTCCAGTCATCGGCCATCTCCACCTTCTGAAGAAGCCCCTCTACCGGACTTTGGCGAAGATCTCGAACCGGTACGGCCCGGTTGTCTTTCTCCGGTTCGGATCACGCCAAGTTCTCGTGGTGTCGTCTCCTTCCGCGGCCGAAGAATGTCTGACCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTCCTCCTATCCAAGTGGTTCGGATACAACAACACGAACCTCGTCTGGTCATCGTATGGGGACCACTGGCGCAACCTCCGCCGAATCTCCACCGTTGAAGTCTTGTCCACTCATCGCCTTCAGATGCTCTCGACCGTCCGTCTGGAAGAGGTCAACTCTTTGATTCGGAGGCTCGCTAGAAGCGAAACCCAGGTGCATAACATGAAGAATGCGTTTTTCGATCTGACTTATAACATCATATTGAGGATGATTGTCGGAAAGCGGTTTTACGGTGATGACGTGGACGATGCTGACGAGGCAAGATGTTATAGAGAGTTACAGGCTGAGTCAACTCGATTGAGTGGTAAGTCAAATATAGGAGATTTTATCCCGTTGATGTCGTGGCTGGGATTTGGATCGGCTGTGGAGAATGAGATAATGGAATGTCACAAAAGAAGAGACGCTTTCATGCAGAGTTTGATTGACCAGCAAAGGAAAAGAATGGTTCAAACTGAAACTGGAGATTCCTTTCGCGATGGAAGGAAGAAAACGATGATTGAAGTACTGTTAGAGCTACAAGAATCTCAACCTGAGCAGTACAATGACGAAACTATCAAAGCCTTGATGCTGGTTCTGTTGGCTGCAGGTACGGAAACATCAGTGAACACGATGGAATGGGCCCTATCTCTTATGTTGAACCAACCAGAAGTTTTAAAGAAAGCGCAGGTCGAAATTGACTGTCAAGTTGGCCAAGACCGTCTGATTGACGAATCTGACGTGGCGAACCTTCCCTACCTACGTGGCATCATCAACGAAACCTTACGAATGTACCCTCCAGGTCCACTGATGGTACCCCACGAGTCATCAAAAGACTGTAGCGTTGGAGGATACCATATCCCACGTGGCACTATGCTGTACGTGAATTTATGGGCGATTCATAACGACCCCAAGAATTGGGGTGAGCCGAGAAAGTTTGATCCCGAGAGGTTTGAGAGGGTGGAAAGTGAGAAGTATGGGTTCAATCTGATGCCGTTCGGGGCGGGTAGAAGGGGCTGTCCAGGAGAGGGATTGGGGCTTCGGGTGATTGGGCTTGTAATGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGCGAGGAGTTGGTGGATATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACGCTTGCTCGCTAAATGTACGCCTCGACCCATTGTCGAACATCTTGATCTTTCTCCAACCAGATCCAACCCGGCCCATTATAAAAATTGA

Protein sequence

MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAHYKN

Homology

[Close]

BLAST of Lcy12g019250 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 4.6e-134
Identity = 250/501 (49.90%), Postives = 334/501 (66.67%), Query Frame = 0


Query: 9   LYIPLFLVLYTLTEQFL---HKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGP 68
           LY  L +VL +++ +      + RNLPP+P  ++PVIGHLHL+ K L+R+L  +S +YG 
Sbjct: 7   LYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGS 66

Query: 69  VVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWR 128
           V  L+ G+R VLVVSSP+AAEEC TKNDIVFANRP  +L K+ GYN T +V S YG+HWR
Sbjct: 67  VFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWR 126

Query: 129 NLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIIL 188
           NLRR++ VE+ S   L    ++R +EV  L+  L +S  Q      MK+   +L++N+ +
Sbjct: 127 NLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTM 186

Query: 189 RMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIME 248
           RM+ GKR++G DV D+DEA+ +R L  E    +G SN GDF+P + W+ F +  E ++ +
Sbjct: 187 RMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKN-YEKKVSK 246

Query: 249 CHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKAL 308
             +  DAF+Q LI + R             +    TMI+ LL LQESQPE Y D+ IK +
Sbjct: 247 ISQEMDAFLQRLIHESR------------INKNNVTMIDHLLSLQESQPEYYTDQIIKGI 306

Query: 309 MLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRG 368
           ++VLL AGT+TS  T+EWA+SL+LN PE L+KA+ EI+ QVG +RLI+E D+  L YL  
Sbjct: 307 IMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHN 366

Query: 369 IINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPE 428
           II+ET R+ P  P++VPHESS DC V GY +P+GT+L VN WAIH DP+ W EP  F PE
Sbjct: 367 IISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPE 426

Query: 429 RFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEG 488
           R   VE E     LMPFG GRR CPG GL  RV+GL +G+LIQCFEW+R GE  +DM EG
Sbjct: 427 RHGGVELEP--SKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEG 486

Query: 489 IAVSMPKAQRLLAKCTPRPIV 504
             ++MPKAQ L A C PR I+
Sbjct: 487 SGLTMPKAQPLEALCKPRNIL 491

[Close]

BLAST of Lcy12g019250 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.2e-127
Identity = 244/510 (47.84%), Postives = 331/510 (64.90%), Query Frame = 0


Query: 7   IFLYIPLFLVLYTLTEQFLH-KIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS----- 66
           + LY    L+   ++ +FL  K +NLPP+P   +P+IGHL LLK P++RTL   S     
Sbjct: 8   VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67

Query: 67  NRYGPVVFLRFGSRQVLVVSSPS-AAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSS 126
           N  G V+ LR GSR V VVSS   AAEEC  KND+V ANRP++++ K  GYNNTN++ + 
Sbjct: 68  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127

Query: 127 YGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLAR---SETQVHNMKNAFFDL 186
           YGDHWRNLRR+ T+E+ STHRL     VR +EV  LI RL+R   ++  V  +K    DL
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187

Query: 187 TYNIILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAV 246
           T+N I+RM+ GKR+YG++  D +EA+  R+L A+    +   N  D++P+   L   S+ 
Sbjct: 188 TFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI---LRLFSSY 247

Query: 247 ENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYND 306
           EN + +  +  D F+Q LID +R    Q ETG         TMI+ LL LQ+S  E Y D
Sbjct: 248 ENRVKKLGEETDKFLQGLIDDKRG---QQETG--------TTMIDHLLVLQKSDIEYYTD 307

Query: 307 ETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVAN 366
           + IK ++L+++ AGT TS  T+EWALS +LN P+V+ KA+ EID +VG DRLI+E+D++ 
Sbjct: 308 QIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSE 367

Query: 367 LPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEP 426
           LPYL+ I+ ETLR++P  PL+VPH +S+DC +G Y +PRGT L VN WAIH DP  W +P
Sbjct: 368 LPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDP 427

Query: 427 RKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEEL 486
             F PERFE+ E  +    L+ FG GRR CPG GL  R++GL +GSLIQCFEWER G   
Sbjct: 428 DSFKPERFEKEEEAQ---KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVE 487

Query: 487 VDMTEGIAVSMPKAQRLLAKCTPRPIVEHL 507
           VDM EG+  ++PKA  L A C  RP +  +
Sbjct: 488 VDMKEGVGNTVPKAIPLKAICKARPFLHKI 500

[Close]

BLAST of Lcy12g019250 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 5.5e-127
Identity = 239/500 (47.80%), Postives = 322/500 (64.40%), Query Frame = 0


Query: 9   LYIPLFLVLYTLTEQF--LHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGPV 68
           L I LF ++ TL   F    K +NLPP P   +P+IG+LH LK+PL+ T   +S +YG +
Sbjct: 8   LIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKYGQI 67

Query: 69  VFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRN 128
             L FGSR V+VVSS + A+EC TKNDIV ANRP  L  K+ GYNNT +  S YGDHWRN
Sbjct: 68  FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 127

Query: 129 LRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSE----TQVHNMKNAFFDLTYNIIL 188
           LRRI ++E+LS+HRL     +R +E+  LI++LA+      T+V  ++  F ++T+N I+
Sbjct: 128 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEV-ELRPMFSEMTFNTIM 187

Query: 189 RMIVGKRFYGDDVD--DADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 248
           RM+ GKR+YG+D D  D +EAR +R +  E   L G +N+GDF+  + W  F   +E  +
Sbjct: 188 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDF-DGLEKRL 247

Query: 249 MECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIK 308
            +  KR DAF+Q LID+ R           F      TMI+ LL  Q+SQPE Y D+ IK
Sbjct: 248 KKISKRTDAFLQGLIDEHR-----------FGKRNSNTMIDHLLTQQQSQPEYYTDQIIK 307

Query: 309 ALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYL 368
            LM+V+L AGT+TS  T+EWA+S +LN PE++KKA+ E+D  +G DR +DE D++ LPYL
Sbjct: 308 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 367

Query: 369 RGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFD 428
           + I+ ETLR++   PL+VPH SS+D S+GGY+IP+ T+L VN W IH DP  W +P  F 
Sbjct: 368 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 427

Query: 429 PERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMT 488
           PERFE+   E     L+ FG GRR CPGE L  R  GL +G LIQCFEW+R GEE +DM 
Sbjct: 428 PERFEK---EGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMV 487

Query: 489 EGIAVSMPKAQRLLAKCTPR 501
           E   ++  K   L A C  R
Sbjct: 488 EAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of Lcy12g019250 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 7.1e-127
Identity = 238/507 (46.94%), Postives = 334/507 (65.88%), Query Frame = 0


Query: 10  YIPLFLVLYTLTEQFLHKI-----RNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG 69
           Y  L  + + +T + L KI     +NLPP P P++P+IG+LH LK PL+RT   +S  YG
Sbjct: 4   YYSLLSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYG 63

Query: 70  PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHW 129
            +  L FGSR V+VVSSPS A EC TKNDI+ ANRPR L  K+  YN T L  +SYGDHW
Sbjct: 64  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 123

Query: 130 RNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLAR---SE----TQVHNMKNAFFDLT 189
           RNLRRI+T++VLS +RL     VR +E N LI++L +   SE    T+V  ++    ++T
Sbjct: 124 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKV-ELRPRLTEMT 183

Query: 190 YNIILRMIVGKRFYGD--DVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSA 249
           +N ++RMI GKR+YGD  DV D +EA+ +RE+ +E   L G +N GDF+PL+  +   + 
Sbjct: 184 FNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN- 243

Query: 250 VENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYN 309
           +E       KR +AF++ LI++ R+  + ++ G         TMI+ LL+L ESQPE Y+
Sbjct: 244 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGG---------TMIDHLLKLSESQPEYYS 303

Query: 310 DETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVA 369
           D  IK L+  +L AGT+TS  T+EW +S +LN PEVLKKA+ E+D Q+G+++L+DE D++
Sbjct: 304 DHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLS 363

Query: 370 NLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGE 429
            LPYL+ II+ETLR++PP PL++PH SS+DC++G +++P+ T++  N+W IH DPK+W +
Sbjct: 364 KLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWND 423

Query: 430 PRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEE 489
              F PERFE+ E       +M FG GRR CPG  L  R +G  +G LIQCFEWER  EE
Sbjct: 424 ALSFKPERFEKEEEVN---KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEE 483

Query: 490 LVDMTEGIAVSMPKAQRLLAKCTPRPI 503
            +DM EG  ++MP    L A C   PI
Sbjct: 484 KLDMMEGKGITMPMKIPLRAMCKALPI 495

[Close]

BLAST of Lcy12g019250 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 450.7 bits (1158), Expect = 2.3e-125
Identity = 236/516 (45.74%), Postives = 335/516 (64.92%), Query Frame = 0


Query: 8   FLYIP-LFLV-----LYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISN 67
           F+ +P LFLV     LY+ T++F     NLPP P PS P +GHLHL+K P++R L + SN
Sbjct: 4   FILLPLLFLVISYKFLYSKTQRF-----NLPPGP-PSRPFVGHLHLMKPPIHRLLQRYSN 63

Query: 68  RYGPVVFLRFGSRQVLVVSSPSAAEECLT-KNDIVFANRPRLLLSKWFGYNNTNLVWSSY 127
           +YGP+  LRFGSR+V+V++SPS A+E  T +NDIV ++RP  L +K+  YN+T +  + Y
Sbjct: 64  QYGPIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPY 123

Query: 128 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAF------- 187
           GDHWRNLRRI + E+LS+HRL     +R +E+  ++ RL+R  TQ  N  N F       
Sbjct: 124 GDHWRNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSR-YTQTSNESNDFTHIELEP 183

Query: 188 --FDLTYNIILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLG 247
              DLT+N I+RM+ GKR+YGDDV++ +EA  +++L  +    SG ++  D++P++    
Sbjct: 184 LLSDLTFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKL-- 243

Query: 248 FGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQP 307
           FG+  E E+    K  D  +Q L+D+ R            RD    TM+  L+ LQ+ QP
Sbjct: 244 FGNKFEKEVKAIGKSMDDILQRLLDECR------------RDKEGNTMVNHLISLQQQQP 303

Query: 308 EQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDE 367
           E Y D  IK LM+ ++ AGTETS  T+EWA++ +L  PEVL+KA+ EID ++G+DRLIDE
Sbjct: 304 EYYTDVIIKGLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDE 363

Query: 368 SDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPK 427
           SD+A LPYL+ +++ET R++P  P ++P   + D  +GGY +PR T++ VN WAIH DP+
Sbjct: 364 SDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPE 423

Query: 428 NWGEPRKFDPERF-ERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWE 487
            W EP KF+P+R+ +   S+ Y + LMPFG GRR CPG GLG R++ L +GSLIQCFEWE
Sbjct: 424 IWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWE 483

Query: 488 RPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIVEHL 507
               E +DM+E   + M K   L A C PRPI+  L
Sbjct: 484 NVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSKL 498

[Close]

BLAST of Lcy12g019250 vs. ExPASy TrEMBL
Match: A0A6J1FJC9 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445994 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.3e-248
Identity = 415/514 (80.74%), Postives = 472/514 (91.83%), Query Frame = 0


Query: 4   LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 63
           +GAI +YIPLF VLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 123
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIILR 183
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLA+ E QV +M NAFFDLT+N++LR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLR 180

Query: 184 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 243
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMEC 240

Query: 244 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 303
           H++RDAFMQ+LI+Q + R ++TE  +SFRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 304 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGI 363
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 364 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 423
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 424 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGI 483
           F +VESEK GF LMPFG+GRRGCPGEGLGLRVIGLV+GSLIQCFEWERPGEELVDMTEG+
Sbjct: 421 FVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGV 480

Query: 484 AVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAH 518
           AVSMPKA+ L AKC PRPIV+ L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

[Close]

BLAST of Lcy12g019250 vs. ExPASy TrEMBL
Match: A0A6J1J228 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.9e-248
Identity = 414/514 (80.54%), Postives = 472/514 (91.83%), Query Frame = 0


Query: 4   LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 63
           +GAI LYIPLFLVLY LTEQF+HKIRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 123
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIILR 183
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLAR E QV +M NAFFDLT+N++LR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180

Query: 184 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 243
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMEC 240

Query: 244 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 303
           H++RDAFMQ+LI+Q + R ++TE  + FRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 304 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGI 363
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 364 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 423
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 424 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGI 483
           F +V+SEK GF L+PFG+GRRGCPGEGLGLRVIGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480

Query: 484 AVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAH 518
           AVSMPKA+ L AKC PRPIV+ L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

[Close]

BLAST of Lcy12g019250 vs. ExPASy TrEMBL
Match: A0A6J1CYI5 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.5e-241
Identity = 415/525 (79.05%), Postives = 473/525 (90.10%), Query Frame = 0


Query: 1   MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 60
           +  +GAIFLY PLFLVLY LTE FLHKIRNLPPTPFPS+P+IGHL+LL KP+YRTLA IS
Sbjct: 6   LAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS 65

Query: 61  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 120
           NR+GPVVFLRFGSR VLVVSSPS AEECLTKND+VFANRPRLL+SK FGYNNTNLVWSSY
Sbjct: 66  NRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSY 125

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQ--VHNMKNAFFDLTY 180
           GDHWR+LRRISTVEVLSTHRLQ+LSTVRLEEV SLIR+LA+SE Q  +  M+++FFDLT+
Sbjct: 126 GDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTF 185

Query: 181 NIILRMIVGKRFYGDDVDDADE-ARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVE 240
           N++LRMI GKRFYGDDVDDADE A+ +RELQAESTRL+G SNIGDF+P+M+WLGFG +VE
Sbjct: 186 NVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVE 245

Query: 241 NEIMECHKRRDAFMQSLIDQQRKRM---VQTETGDSFRDGRKKTMIEVLLELQESQPEQY 300
            ++M+CH +RDAFMQ+LI++QRKR     ++E  DSFRDGR+KTMIEVLLELQES+P+ Y
Sbjct: 246 KKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHY 305

Query: 301 NDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDV 360
            DE+IKALMLVLL AGTETSVNTMEWALSL+LN PEVLKKAQVEID QVGQDRLIDESD+
Sbjct: 306 TDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDM 365

Query: 361 ANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWG 420
           A+LPYLRGII ET RMYPP PLMVPH+SS+DCSVGG+HIPRGTMLYVNLWAIHNDPK W 
Sbjct: 366 AHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWA 425

Query: 421 EPRKFDPERFERVESEK--YGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERP 480
            P KFDP+RF+R+ES+K  YGFNLMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERP
Sbjct: 426 NPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERP 485

Query: 481 GEELVDMTEGIAVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAH 518
           GE+LVDMTEGIAVSMPKAQ L AKC PRPI+ +L  S T++N AH
Sbjct: 486 GEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529

[Close]

BLAST of Lcy12g019250 vs. ExPASy TrEMBL
Match: A0A1S3BUR6 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.3e-237
Identity = 399/516 (77.33%), Postives = 451/516 (87.40%), Query Frame = 0


Query: 1   MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 60
           MGVLG+IF+Y PLFLVLY L E  LHKIRNLPPTPFP +P+IGHLHLLKKP+YRTLAKIS
Sbjct: 1   MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60

Query: 61  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 120
           N YGPVV+ RFGSR+VLVVSS  AAEECLTKND+VFANRPRL++SKWFGYNNTNL+WSSY
Sbjct: 61  NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 180
           GDHWRNLRRIST+E+LSTHRLQM S+VRLEEV SLIRRLA  E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180

Query: 181 ILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 240
           +LRM+VGKR+YG+DVDD DEA+ +R+LQ +  +L GKS + DFIPL+SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240

Query: 241 MECHKRRDAFMQSLIDQQRKR-MVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 300
           +E H +RD FMQ+LIDQ +KR   Q  +   ++DGR+KTMIEVLLELQ+ +P QY DETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300

Query: 301 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPY 360
           +ALML+LLAAGTETSV TMEWALSLMLN PE LKK Q EID QVG DRLIDESD+ANLP 
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360

Query: 361 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 420
           LRGIINET RMYPP PLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHNDPK W  PRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420

Query: 421 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDM 480
           +P RFE +ESEK+GFNLMPFG+GRRGCPGEGLGLRVIGLV+GSL+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480

Query: 481 TEGIAVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNP 516
           TEGIA++MPKA  L AKCTPRPIV  L LS   SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516

[Close]

BLAST of Lcy12g019250 vs. ExPASy TrEMBL
Match: A0A0A0L2I7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 2.2e-235
Identity = 392/516 (75.97%), Postives = 453/516 (87.79%), Query Frame = 0


Query: 1   MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 60
           MGVL +IF+YIPLF VLYTL E  +HKIRNLPPTPFP +PVIGHLHLLK P+YRTLA+IS
Sbjct: 1   MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60

Query: 61  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 120
           N YGPVV+ RFG R+VLVVSSP AAEECLTKNDIVFANRPRL++SKWFGYNNTNLVWSSY
Sbjct: 61  NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPRLIISKWFGYNNTNLVWSSY 120

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 180
           GDHWRNLRRIST+E+LSTHR+QMLS  RLEEV SLIRRLA +E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNENQIHNMKNEFFDLTYNV 180

Query: 181 ILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 240
           +LRM+VGKRFYG+DVDD DEA+ +R+LQ +  +L GKS + DFIP +SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240

Query: 241 MECHKRRDAFMQSLIDQQRKRMV-QTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 300
           +ECH +RD FMQ+LIDQ +KR+V Q ++ +  +DGR+KTMIEVLLELQ+  P+QY DETI
Sbjct: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300

Query: 301 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPY 360
           +ALMLVLLAAGTETSV  MEWALSLMLN P+ LKK Q EID QVG DRLIDESD+ANLP 
Sbjct: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360

Query: 361 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 420
           LRGIINETLRMYPP PL+VPHESSKDC++GGYHIPRGT+L+VNLWAIHNDPK W  PRKF
Sbjct: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420

Query: 421 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDM 480
           +P RFE +E+EK+GFNL+PFG+GRRGCPGEGL LRVIGLV+G+L+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480

Query: 481 TEGIAVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNP 516
           TEG+A++MPKA  L AKCTPRPIV  L LS   SNP
Sbjct: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 516

[Close]

BLAST of Lcy12g019250 vs. NCBI nr
Match: XP_022940352.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 868.6 bits (2243), Expect = 2.7e-248
Identity = 415/514 (80.74%), Postives = 472/514 (91.83%), Query Frame = 0


Query: 4   LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 63
           +GAI +YIPLF VLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 123
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIILR 183
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLA+ E QV +M NAFFDLT+N++LR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLR 180

Query: 184 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 243
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMEC 240

Query: 244 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 303
           H++RDAFMQ+LI+Q + R ++TE  +SFRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 304 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGI 363
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 364 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 423
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 424 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGI 483
           F +VESEK GF LMPFG+GRRGCPGEGLGLRVIGLV+GSLIQCFEWERPGEELVDMTEG+
Sbjct: 421 FVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGV 480

Query: 484 AVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAH 518
           AVSMPKA+ L AKC PRPIV+ L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

[Close]

BLAST of Lcy12g019250 vs. NCBI nr
Match: XP_022982225.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 866.7 bits (2238), Expect = 1.0e-247
Identity = 414/514 (80.54%), Postives = 472/514 (91.83%), Query Frame = 0


Query: 4   LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 63
           +GAI LYIPLFLVLY LTEQF+HKIRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 123
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIILR 183
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLAR E QV +M NAFFDLT+N++LR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180

Query: 184 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 243
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMEC 240

Query: 244 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 303
           H++RDAFMQ+LI+Q + R ++TE  + FRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 304 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGI 363
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 364 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 423
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 424 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGI 483
           F +V+SEK GF L+PFG+GRRGCPGEGLGLRVIGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480

Query: 484 AVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAH 518
           AVSMPKA+ L AKC PRPIV+ L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

[Close]

BLAST of Lcy12g019250 vs. NCBI nr
Match: XP_023523281.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.4 bits (2219), Expect = 1.6e-245
Identity = 411/514 (79.96%), Postives = 470/514 (91.44%), Query Frame = 0


Query: 4   LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 63
           +GAI +YIPLFLVLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIIVYIPLFLVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 123
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIILR 183
           WRNLRRISTVE+LSTHRLQML+ VR +EV SLI RLAR E QV +M NAFFDLT+N++LR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRSDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180

Query: 184 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 243
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+ DFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMEDFIPLMSWLGLGWAAEKEIMEC 240

Query: 244 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 303
           H++RDAFMQ+LI+Q + R ++TE  +SFRDGRKKT+IEVL+ELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLVELQESDPDQYNDETIRALM 300

Query: 304 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGI 363
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 364 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 423
           INETLRMYPP PLMVPHESSKDCSVGGY IPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYQIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 424 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGI 483
           F +V+SEK+GF LMPFG+GRRGCPGEGLGLRVIGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKHGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480

Query: 484 AVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAH 518
           AVSMPKA+ L AKC PRPIV  L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVARLHLSQTKSNLAY 514

[Close]

BLAST of Lcy12g019250 vs. NCBI nr
Match: XP_022146589.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 845.1 bits (2182), Expect = 3.2e-241
Identity = 415/525 (79.05%), Postives = 473/525 (90.10%), Query Frame = 0


Query: 1   MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 60
           +  +GAIFLY PLFLVLY LTE FLHKIRNLPPTPFPS+P+IGHL+LL KP+YRTLA IS
Sbjct: 6   LAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS 65

Query: 61  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 120
           NR+GPVVFLRFGSR VLVVSSPS AEECLTKND+VFANRPRLL+SK FGYNNTNLVWSSY
Sbjct: 66  NRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSY 125

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQ--VHNMKNAFFDLTY 180
           GDHWR+LRRISTVEVLSTHRLQ+LSTVRLEEV SLIR+LA+SE Q  +  M+++FFDLT+
Sbjct: 126 GDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTF 185

Query: 181 NIILRMIVGKRFYGDDVDDADE-ARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVE 240
           N++LRMI GKRFYGDDVDDADE A+ +RELQAESTRL+G SNIGDF+P+M+WLGFG +VE
Sbjct: 186 NVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVE 245

Query: 241 NEIMECHKRRDAFMQSLIDQQRKRM---VQTETGDSFRDGRKKTMIEVLLELQESQPEQY 300
            ++M+CH +RDAFMQ+LI++QRKR     ++E  DSFRDGR+KTMIEVLLELQES+P+ Y
Sbjct: 246 KKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHY 305

Query: 301 NDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDV 360
            DE+IKALMLVLL AGTETSVNTMEWALSL+LN PEVLKKAQVEID QVGQDRLIDESD+
Sbjct: 306 TDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDBQVGQDRLIDESDM 365

Query: 361 ANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWG 420
           A+LPYLRGII ET RMYPP PLMVPH+SS+DCSVGG+HIPRGTMLYVNLWAIHNDPK W 
Sbjct: 366 AHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWA 425

Query: 421 EPRKFDPERFERVESEK--YGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERP 480
            P KFDP+RF+R+ES+K  YGFNLMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERP
Sbjct: 426 NPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERP 485

Query: 481 GEELVDMTEGIAVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNPAH 518
           GE+LVDMTEGIAVSMPKAQ L AKC PRPI+ +L  S T++N AH
Sbjct: 486 GEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529

[Close]

BLAST of Lcy12g019250 vs. NCBI nr
Match: XP_008453068.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 831.2 bits (2146), Expect = 4.8e-237
Identity = 399/516 (77.33%), Postives = 451/516 (87.40%), Query Frame = 0


Query: 1   MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 60
           MGVLG+IF+Y PLFLVLY L E  LHKIRNLPPTPFP +P+IGHLHLLKKP+YRTLAKIS
Sbjct: 1   MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60

Query: 61  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 120
           N YGPVV+ RFGSR+VLVVSS  AAEECLTKND+VFANRPRL++SKWFGYNNTNL+WSSY
Sbjct: 61  NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 180
           GDHWRNLRRIST+E+LSTHRLQM S+VRLEEV SLIRRLA  E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180

Query: 181 ILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 240
           +LRM+VGKR+YG+DVDD DEA+ +R+LQ +  +L GKS + DFIPL+SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240

Query: 241 MECHKRRDAFMQSLIDQQRKR-MVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 300
           +E H +RD FMQ+LIDQ +KR   Q  +   ++DGR+KTMIEVLLELQ+ +P QY DETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300

Query: 301 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPY 360
           +ALML+LLAAGTETSV TMEWALSLMLN PE LKK Q EID QVG DRLIDESD+ANLP 
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360

Query: 361 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 420
           LRGIINET RMYPP PLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHNDPK W  PRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420

Query: 421 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDM 480
           +P RFE +ESEK+GFNLMPFG+GRRGCPGEGLGLRVIGLV+GSL+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480

Query: 481 TEGIAVSMPKAQRLLAKCTPRPIVEHLDLSPTRSNP 516
           TEGIA++MPKA  L AKCTPRPIV  L LS   SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516

[Close]

BLAST of Lcy12g019250 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 472.6 bits (1215), Expect = 4.0e-133
Identity = 249/509 (48.92%), Postives = 332/509 (65.23%), Query Frame = 0


Query: 1   MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 60
           M  L  IF +  + L L  L  +   K+ NLPP+P  ++PVIGHL LLK PL+R    +S
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRKL-NLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 61  NRYG--PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWS 120
              G  P++ LR G+R + VVSS S AEEC TKND++ ANR   + +K   Y N+ +V +
Sbjct: 61  QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120

Query: 121 SYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSE----TQVHNMKNAFF 180
           SY +HWRNLRRI  +E+ S HRL   S++R +E+  LI RL R+     T+V  MK+ F 
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKV-EMKSMFS 180

Query: 181 DLTYNIILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGS 240
           DLT+N I+RM+ GK +YGD  +D  EA+  R L AE+   SG  N  D+IP+++W+ +  
Sbjct: 181 DLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS- 240

Query: 241 AVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQY 300
             E  I +   R D F+Q L+D++R+           ++ ++ TM++ LL LQE+QPE Y
Sbjct: 241 --ETRIKKLAGRLDEFLQGLVDEKREG----------KEKKENTMVDHLLCLQETQPEYY 300

Query: 301 NDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDV 360
            D  IK  ML L+A GT+T+  T+EWALS +LN PEVL KA+ EID  +G DRL++ESD+
Sbjct: 301 MDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDI 360

Query: 361 ANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWG 420
            NLPYL+ I++ETLR+YP  P+++PH +SKDC VGGY +PRGTML  N WAIH DP  W 
Sbjct: 361 PNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWD 420

Query: 421 EPRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGE 480
           +P  F PERFE+    K    LMPFG GRR CPG GL  R++ L +GSLIQCFEWER GE
Sbjct: 421 DPTSFKPERFEKEGEAK---KLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE 480

Query: 481 ELVDMTEGIAVSMPKAQRLLAKCTPRPIV 504
           E VDMTEG  ++MPKA+ L A C  R  V
Sbjct: 481 EEVDMTEGPGLTMPKARPLEAMCRARDFV 491

[Close]

BLAST of Lcy12g019250 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 470.7 bits (1210), Expect = 1.5e-132
Identity = 250/502 (49.80%), Postives = 330/502 (65.74%), Query Frame = 0


Query: 8   FLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG--P 67
           FL+I L L   T     + +  NLPP+P  ++PVIGHL LLK PL+R    +S   G  P
Sbjct: 10  FLFISLSL---TFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAP 69

Query: 68  VVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWR 127
           ++ LR G+R V VVSS S AEEC TKND+V ANR   L SK   Y  T +V +SYGDHWR
Sbjct: 70  IISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWR 129

Query: 128 NLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARS---ETQVHNMKNAFFDLTYNIIL 187
           NLRRI  VE+ S HRL   S++R +E++ LI  L+R+   E     MK+ F +LT+N I+
Sbjct: 130 NLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNII 189

Query: 188 RMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIME 247
           RM+ GK +YGD  +D  EA+  REL AE     G  N  D++P+++W+   +  E  I +
Sbjct: 190 RMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIKK 249

Query: 248 CHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKAL 307
              R D F+Q L+D++R+           ++ R+ TM++ LL LQE+QPE Y D  IK +
Sbjct: 250 IASRLDEFLQGLVDERREG----------KEKRQNTMVDHLLCLQETQPEYYTDNIIKGI 309

Query: 308 MLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRG 367
           ML L+ AGT+TS  T+EW LS +LN P++L KA+ EID +VG +RL++ESD+++LPYL+ 
Sbjct: 310 MLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQN 369

Query: 368 IINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPE 427
           I++E+LR+YP  PL+VPH +S+DC VGGYH+PRGTML  N WAIH DPK W +P  F PE
Sbjct: 370 IVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPE 429

Query: 428 RFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTE- 487
           RFE+   E     L+ FG GRR CPG GL  R+  L +GSLIQCFEWER GEE VDMTE 
Sbjct: 430 RFEK---EGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEG 489

Query: 488 GIAVSMPKAQRLLAKCTPRPIV 504
           G  V MPKA  L+A C  RP+V
Sbjct: 490 GGGVIMPKAIPLVAMCKARPVV 492

[Close]

BLAST of Lcy12g019250 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 465.3 bits (1196), Expect = 6.4e-131
Identity = 241/479 (50.31%), Postives = 323/479 (67.43%), Query Frame = 0


Query: 30  NLPPTPFPSVPVIGHLHLLKKPLYRTLAKISN--RYGPVVFLRFGSRQVLVVSSPSAAEE 89
           NLPP+P  S+PVIGHL LLK P++RT   +S      P+  LR G+R V V SS S AEE
Sbjct: 29  NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEE 88

Query: 90  CLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLRRISTVEVLSTHRLQMLSTV 149
           C TKND+V ANRP  +L+K   Y+ T ++ +SYGDHWRNLRRI +VE+ S HRL    ++
Sbjct: 89  CFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSI 148

Query: 150 RLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIILRMIVGKRFYGDDVDDADEARCY 209
           R +E+  L+ RL+R+ +Q     +MK+   DLT+N ILRM+ GKR+YGD V+D  EA+  
Sbjct: 149 RKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRV 208

Query: 210 RELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQ 269
           R+L A+    +G  N  D++P++  +   S  E  + +   R D F+Q L+D++R+    
Sbjct: 209 RQLIADVVACAGAGNAVDYLPVLRLV---SDYETRVKKLAGRLDEFLQGLVDEKRE---A 268

Query: 270 TETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLVLLAAGTETSVNTMEWALSL 329
            E G+        TMI+ LL LQESQP+ + D  IK  ML L+ AGT+TS  T+EWALS 
Sbjct: 269 KEKGN--------TMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSN 328

Query: 330 MLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGIINETLRMYPPGPLMVPHESSK 389
           +LN P+VL KA+ EID ++G DRL+DESD++NLPYL+ I++ETLR+YP  P+++PH +S+
Sbjct: 329 VLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASE 388

Query: 390 DCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFERVESEKYGFNLMPFGAGRR 449
           DC V GY +PRGT+L  N+WAIH DP+ W +P  F PERFE+   E     LMPFG GRR
Sbjct: 389 DCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK---EGEAQKLMPFGLGRR 448

Query: 450 GCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIV 504
            CPG GL  R+I L +GSLIQC EWE+ GEE VDM+EG  V+MPKA+ L A C  RP V
Sbjct: 449 ACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMCRARPSV 489

[Close]

BLAST of Lcy12g019250 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 461.1 bits (1185), Expect = 1.2e-129
Identity = 239/506 (47.23%), Postives = 330/506 (65.22%), Query Frame = 0


Query: 3   VLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNR 62
           +L  +FL I + L+L     +      NLPP+P   +PVIGHLHLLK+P++RT   IS  
Sbjct: 8   LLSFLFLTISIKLLLTKSNRK-----PNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKS 67

Query: 63  YG--PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 122
            G  P+  LR G+R V V+SS S AEEC TKND+V ANRP ++++K  GYN TN++ +SY
Sbjct: 68  LGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASY 127

Query: 123 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLT 182
           GDHWRNLRRI+ VE+ S+HR+   S++R +E+  LI  L+R          +K+   +L 
Sbjct: 128 GDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLA 187

Query: 183 YNIILRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVE 242
           +N I+ M+ GKR+YG   +D DEA+  REL AE    +G  N+ D++P ++W+   +  E
Sbjct: 188 FNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFE 247

Query: 243 NEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDE 302
           N+      R D  +Q L+D++R    + E G        +T+I+ LL  QE++PE Y D 
Sbjct: 248 NQTKILGNRLDRVLQKLVDEKR---AEKEKG--------QTLIDHLLSFQETEPEYYTDV 307

Query: 303 TIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANL 362
            IK ++L L+ AGT+TS  T+EWA+S +LN PE+L+KA+ EID ++G DRL++ESD+ NL
Sbjct: 308 IIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNL 367

Query: 363 PYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPR 422
            YL+ I++ETLR+YP  PL++PH SS +C V GY +PR T+L  N+WA+H DP  W EP 
Sbjct: 368 HYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPE 427

Query: 423 KFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELV 482
           +F PERFE+   E     LMPFG GRR CPG  LG R++ L +G LIQ FEWER G ELV
Sbjct: 428 RFKPERFEK---EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELV 487

Query: 483 DMTEGIAVSMPKAQRLLAKCTPRPIV 504
           DMTEG  ++MPKA  L A C  R IV
Sbjct: 488 DMTEGEGITMPKATPLRAMCKARAIV 491

[Close]

BLAST of Lcy12g019250 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 460.3 bits (1183), Expect = 2.1e-129
Identity = 247/502 (49.20%), Postives = 325/502 (64.74%), Query Frame = 0


Query: 14  FLVLYTLTEQFLHKIR---NLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG--PVVF 73
           FL L+TL    L + +   NLPP+P  S+PVIGHLHLLK PL+RT   +S   G  PV  
Sbjct: 11  FLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFH 70

Query: 74  LRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLR 133
           LR G+R V V+SS S AEEC TKND+V ANRP+  +SK  GYN T L+ +SYGDHWRNLR
Sbjct: 71  LRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLR 130

Query: 134 RISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIILRMI 193
           RI+ VE+ STHRL     +R +E+  LI  L+R          MK    +L  N  +RM+
Sbjct: 131 RIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRML 190

Query: 194 VGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHK 253
            GKR++G+D DD   A+  + L +E+   +G  N  D++ ++ W+   S+ E  I     
Sbjct: 191 AGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYEKRIKNLGN 250

Query: 254 RRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLV 313
           R D F+Q L+D++R    + E G+        TMI+ LL LQ+ QP+ Y D  IK ++L 
Sbjct: 251 RFDTFLQKLVDEKR---AEKEKGE--------TMIDHLLALQDIQPDYYTDVIIKGIILT 310

Query: 314 LLAAGTETSVNTMEWALSLMLNQPEVLKKAQVEIDCQVGQDRLIDESDVANLPYLRGIIN 373
           L+ AGT+TS  T+EWA+S +LN PE+LKKA++EID +VG DRL+DESD+ NL YL+ I+ 
Sbjct: 311 LIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVL 370

Query: 374 ETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFE 433
           ETLRMYP  PL++PH SS+DC VGGY IP GTM+  N WA+H DP+ W +P  F PERFE
Sbjct: 371 ETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFE 430

Query: 434 RV-ESEKYGFNLMPFGAGRRGCPGEGLGLRVIGLVMGSLIQCFEWERPGEELVDMTEGIA 493
           +  E+EK    L+ FG GRR CPG GL  R+I   +GSL+QCFEWER GE+ VDMTE   
Sbjct: 431 KEGEAEK----LISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKG 490

Query: 494 VSMPKAQRLLAKCTPRPIVEHL 507
            ++PKA  L A C  R IV+ L
Sbjct: 491 ATLPKAIPLRAMCKARSIVDKL 491

The following BLAST results are available for this feature:

BLAST of Lcy12g019250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	4.6e-134	49.90	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9FG65	3.2e-127	47.84	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q6WNQ8	5.5e-127	47.80	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	7.1e-127	46.94	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
O65790	2.3e-125	45.74	Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2	[more]

BLAST of Lcy12g019250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FJC9	1.3e-248	80.74	cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114459...	[more]
A0A6J1J228	4.9e-248	80.54	cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119...	[more]
A0A6J1CYI5	1.5e-241	79.05	cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV...	[more]
A0A1S3BUR6	2.3e-237	77.33	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1	[more]
A0A0A0L2I7	2.2e-235	75.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1	[more]

BLAST of Lcy12g019250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022940352.1	2.7e-248	80.74	cytochrome P450 81D1-like isoform X1 [Cucurbita moschata]	[more]
XP_022982225.1	1.0e-247	80.54	cytochrome P450 81D1-like isoform X1 [Cucurbita maxima]	[more]
XP_023523281.1	1.6e-245	79.96	cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022146589.1	3.2e-241	79.05	cytochrome P450 81D1-like [Momordica charantia]	[more]
XP_008453068.1	4.8e-237	77.33	PREDICTED: cytochrome P450 81D1-like [Cucumis melo]	[more]

BLAST of Lcy12g019250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37360.1	4.0e-133	48.92	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37340.1	1.5e-132	49.80	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37370.1	6.4e-131	50.31	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37320.1	1.2e-129	47.23	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37330.1	2.1e-129	49.20	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 152..172
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 3..470 e-value: 2.4E-19 score: 67.4
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 14..506
None	No IPR available	PANTHER	PTHR47947:SF24	CYTOCHROME P450 81E8	coord: 14..506
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 309..326 score: 34.25 coord: 362..373 score: 40.67 coord: 437..446 score: 59.44 coord: 446..457 score: 31.36
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 33..489 e-value: 1.1E-96 score: 324.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 298..315 score: 31.68 coord: 318..344 score: 37.14 coord: 446..469 score: 33.9 coord: 176..194 score: 32.21 coord: 402..426 score: 39.4 coord: 60..79 score: 37.31 coord: 436..446 score: 57.89 coord: 84..105 score: 27.91 coord: 361..379 score: 31.91
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 26..505 e-value: 1.4E-125 score: 421.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..500
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 439..448

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g019250.1	Lcy12g019250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen