Lcy12g017750 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g017750
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionUbiquitinyl hydrolase 1
LocationChr12: 45322763 .. 45324236 (-)
RNA-Seq ExpressionLcy12g017750
SyntenyLcy12g017750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCATGGATATTAAAAAAAGAAAAATATGAGGTTGGATTTCTGGATGATTCTATTATTGCTCACAAGGGAACTATTCTTTTTTTCTTTTTTAACAGAGAATGGAGACTGCAGATGCATTGCTCACAAGGCCTTCTCTGGACAGTTGAGATCAGATGTCACTTGTATGACCTGTGGGTTCACTTCAACAACTTATGATCCATGTGTGGACATTTCACTTGACTTGGACACCCAGGATTTCTCTTCTAGAAATATGGCTGGCAAGTCGGCAAAGCCAGCGGATTTCAGCAGTAAATCTACGCTATCTGGTTGTTTGGATCTATTCACGAGACCGGAAAAGTTGGGATCGGACCAGAAACTTTTCTGCCAAAATTGTCAAGAAATGAGAAATTCAGTGAAACAAATGTCTATCAGAAAGCTGCCATTGGTCTTGTGTCTGCATATCAAACGATTCGAGCACTCTTTTGTCAGGCGAACATCGAGAAAAATCGATCGGTATTTGCAGTTTCCTTTTTCCCTGGACATGATTCCGTATCTGTCATCTTCTATTATCAGAAACAGATATGGGAATAGGATTTTTGCTTCTGAGGGGGATGAATTAGATGCTTCATCGAAGTTTGAGATTTTCGCTGTGGTTTCTCATTCGGGGACGCTCGAGTCTGGCCATTATGTGACGTACTTGCGTTTGAGAAATCGATGGTATAAATGTGACGATGCCTGGATAACAGAAGTCGATGAAGAAATGGTGAGAAATTCTCAGTGCTACATGATGTTTTATCTACAGAAAATGGCCAACTACAAATCAAATGAGGATGTAAGCTGCCTTCCTATTTCCCCACGCAACGATGCATTCCAACAAATTGCTGGTTGCTGTTAATAGCGGGATTCACCGAAGGTTTCCAGATTATGCATCATACATTCAAGGCCGACTAAGCATCCAAAAGAAAGATCAGATGAACCTCTTCAAGAAATTGTAACCTTGAATGTTCTGTGGTCGTGCCTCTATTGAAATGCCAGAATTTAGTTTGAAGCATTTACACTTGGGAAATGGATCGAACGACATGACCTTAAGTGGGTTGTTGAGGCTCGGCCAACGGGATGATGCGATATCGAAAACGTCGTCGATGTGCAGTATCTACCTACAAAGATGAAGATGCCTACTGCAAAATGTTCATGTGTAATCTCAATTTCACATGCAAATGTGATTTGTAAAAGCATTGATCTCACACAAGGCTTTTTTCTCTTCTTTTAATAAGAAAGCCGAAAGTGTAAGGAATTCAAAAGTTAATCACTTGAAAACTGTAATAAGTTTAAAGGCCCTGCTCATCAAGAGAAGCTCCAGTTTCTTCACAGTTCAAGATTCTTTCTTTTGTTCTTTTGATTCATTAGATTTCCTCTTGGATCAATGAAGAGAATTGTTGAATGCAGACATCAAGCTCAATTGGAAAAAAGTGCTTTTTGGGGTGTGTTTTTG

mRNA sequence

CATTCATGGATATTAAAAAAAGAAAAATATGAGGTTGGATTTCTGGATGATTCTATTATTGCTCACAAGGGAACTATTCTTTTTTTCTTTTTTAACAGAGAATGGAGACTGCAGATGCATTGCTCACAAGGCCTTCTCTGGACAGTTGAGATCAGATGTCACTTGTATGACCTGTGGGTTCACTTCAACAACTTATGATCCATGTGTGGACATTTCACTTGACTTGGACACCCAGGATTTCTCTTCTAGAAATATGGCTGGCAAGTCGGCAAAGCCAGCGGATTTCAGCAGTAAATCTACGCTATCTGGTTGTTTGGATCTATTCACGAGACCGGAAAAGTTGGGATCGGACCAGAAACTTTTCTGCCAAAATTGTCAAGAAATGAGAAATTCAGTGAAACAAATGTCTATCAGAAAGCTGCCATTGGTCTTGTGTCTGCATATCAAACGATTCGAGCACTCTTTTGTCAGGCGAACATCGAGAAAAATCGATCGGTATTTGCAGTTTCCTTTTTCCCTGGACATGATTCCGTATCTGTCATCTTCTATTATCAGAAACAGATATGGGAATAGGATTTTTGCTTCTGAGGGGGATGAATTAGATGCTTCATCGAAGTTTGAGATTTTCGCTGTGGTTTCTCATTCGGGGACGCTCGAGTCTGGCCATTATGTGACGTACTTGCGTTTGAGAAATCGATGGTATAAATGTGACGATGCCTGGATAACAGAAGTCGATGAAGAAATGGTGAGAAATTCTCAGTGCTACATGATGTTTTATCTACAGAAAATGGCCAACTACAAATCAAATGAGGATGTAAGCTGCCTTCCTATTTCCCCACGCAACGATGCATTCCAACAAATTGCTGGTTGCTGTTAATAGCGGGATTCACCGAAGGTTTCCAGATTATGCATCATACATTCAAGGCCGACTAAGCATCCAAAAGAAAGATCAGATGAACCTCTTCAAGAAATTGTAACCTTGAATGTTCTGTGGTCGTGCCTCTATTGAAATGCCAGAATTTAGTTTGAAGCATTTACACTTGGGAAATGGATCGAACGACATGACCTTAAGTGGGTTGTTGAGGCTCGGCCAACGGGATGATGCGATATCGAAAACGTCGTCGATGTGCAGTATCTACCTACAAAGATGAAGATGCCTACTGCAAAATGTTCATGTGTAATCTCAATTTCACATGCAAATGTGATTTGTAAAAGCATTGATCTCACACAAGGCTTTTTTCTCTTCTTTTAATAAGAAAGCCGAAAGTGTAAGGAATTCAAAAGTTAATCACTTGAAAACTGTAATAAGTTTAAAGGCCCTGCTCATCAAGAGAAGCTCCAGTTTCTTCACAGTTCAAGATTCTTTCTTTTGTTCTTTTGATTCATTAGATTTCCTCTTGGATCAATGAAGAGAATTGTTGAATGCAGACATCAAGCTCAATTGGAAAAAAGTGCTTTTTGGGGTGTGTTTTTG

Coding sequence (CDS)

ATGAGGTTGGATTTCTGGATGATTCTATTATTGCTCACAAGGGAACTATTCTTTTTTTCTTTTTTAACAGAGAATGGAGACTGCAGATGCATTGCTCACAAGGCCTTCTCTGGACAGTTGAGATCAGATGTCACTTGTATGACCTGTGGGTTCACTTCAACAACTTATGATCCATGTGTGGACATTTCACTTGACTTGGACACCCAGGATTTCTCTTCTAGAAATATGGCTGGCAAGTCGGCAAAGCCAGCGGATTTCAGCAGTAAATCTACGCTATCTGGTTGTTTGGATCTATTCACGAGACCGGAAAAGTTGGGATCGGACCAGAAACTTTTCTGCCAAAATTGTCAAGAAATGAGAAATTCAGTGAAACAAATGTCTATCAGAAAGCTGCCATTGGTCTTGTGTCTGCATATCAAACGATTCGAGCACTCTTTTGTCAGGCGAACATCGAGAAAAATCGATCGGTATTTGCAGTTTCCTTTTTCCCTGGACATGATTCCGTATCTGTCATCTTCTATTATCAGAAACAGATATGGGAATAGGATTTTTGCTTCTGAGGGGGATGAATTAGATGCTTCATCGAAGTTTGAGATTTTCGCTGTGGTTTCTCATTCGGGGACGCTCGAGTCTGGCCATTATGTGACGTACTTGCGTTTGAGAAATCGATGGTATAAATGTGACGATGCCTGGATAACAGAAGTCGATGAAGAAATGGTGAGAAATTCTCAGTGCTACATGATGTTTTATCTACAGAAAATGGCCAACTACAAATCAAATGAGGATGTAAGCTGCCTTCCTATTTCCCCACGCAACGATGCATTCCAACAAATTGCTGGTTGCTGTTAA

Protein sequence

MRLDFWMILLLLTRELFFFSFLTENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKPADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFEHSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVVSHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDVSCLPISPRNDAFQQIAGCC
Homology
BLAST of Lcy12g017750 vs. ExPASy Swiss-Prot
Match: Q9LEW0 (Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 9.0e-84
Identity = 162/262 (61.83%), Postives = 195/262 (74.43%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGK---S 83
           +N +C+CI HKAFSG LRSDVTC TCG TSTTYDP +DISL LD+ +  S     K   S
Sbjct: 292 DNEECQCITHKAFSGLLRSDVTCTTCGSTSTTYDPFIDISLTLDSMNGFSPADCRKNRYS 351

Query: 84  AKPADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIK 143
             P+  +   TLSGCLD FTR EKLG DQKL CQ+C E R S KQMSIR+LPL+LCLH+K
Sbjct: 352 GGPSVNAIMPTLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVK 411

Query: 144 RFEHSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGD-ELDASS---- 203
           RFEHS  R+TSRKID YLQ+PF L+M PYLSSSII  R+GNRIFA +G+ E D+SS    
Sbjct: 412 RFEHSLTRKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSP 471

Query: 204 --KFEIFAVVSHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK 263
             +FEIFAVV+H G LESGHYVTYLRL+  WY+CDDAWI EV+EE+VR  +CYM+FY Q+
Sbjct: 472 SAEFEIFAVVTHKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQE 531

Query: 264 MANYKSNEDVSCLPISPRNDAF 276
               K+++++S   IS   DAF
Sbjct: 532 TVIQKAHKELSYQVIS-MADAF 552

BLAST of Lcy12g017750 vs. ExPASy Swiss-Prot
Match: Q6DCJ1 (Ubiquitin carboxyl-terminal hydrolase 22-B OS=Xenopus laevis OX=8355 GN=usp22-b PE=2 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 5.2e-47
Identity = 101/237 (42.62%), Postives = 144/237 (60.76%), Query Frame = 0

Query: 28  CRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQD-----FSSRNMAGKSAK 87
           C CI  + F+G L+SDVTC  C   STT DP  DISLDL          S  + AG    
Sbjct: 291 CNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSDAGVVNG 350

Query: 88  PADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRF 147
            +  S  +TL+ CL  FTRPE LGS  K+ C  C   + S KQ++++KLP+V C H+KRF
Sbjct: 351 ESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRF 410

Query: 148 EHSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAV 207
           EHS   +  RKI  Y+ FP  LDM+P+++SS  +    N  +    D L   +K+ +FAV
Sbjct: 411 EHS--AKLRRKITTYVSFPLELDMMPFMASS--KESRMNGQYQQPSDSLHNDNKYSLFAV 470

Query: 208 VSHSGTLESGHYVTYLRL-RNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYK 259
           V+H GTLESGHY +++R  +++W+KCDDA IT+   + V +S+ Y++FY ++   Y+
Sbjct: 471 VNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVIDSEGYLLFYHKQFLEYE 523

BLAST of Lcy12g017750 vs. ExPASy Swiss-Prot
Match: Q6GNI6 (Ubiquitin carboxyl-terminal hydrolase 22-A OS=Xenopus laevis OX=8355 GN=usp22-a PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 6.7e-47
Identity = 101/237 (42.62%), Postives = 143/237 (60.34%), Query Frame = 0

Query: 28  CRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQD-----FSSRNMAGKSAK 87
           C CI  + F+G L+SDVTC  C   STT DP  DISLDL          S  + AG    
Sbjct: 291 CNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSDAGVVNG 350

Query: 88  PADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRF 147
            +  S  +TL+ CL  FTRPE LGS  K+ C  C   + S KQ++++KLP+V C H+KRF
Sbjct: 351 ESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRF 410

Query: 148 EHSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAV 207
           EHS   +  RKI  Y+ FP  LDM P+++SS  +    N  +    D L   +K+ +FAV
Sbjct: 411 EHS--AKLRRKITTYVSFPLELDMTPFMASS--KESRMNGQYQQPSDSLHNDNKYSLFAV 470

Query: 208 VSHSGTLESGHYVTYLRL-RNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYK 259
           V+H GTLESGHY +++R  +++W+KCDDA IT+   + V +S+ Y++FY ++   Y+
Sbjct: 471 VNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 523

BLAST of Lcy12g017750 vs. ExPASy Swiss-Prot
Match: A6H8I0 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 5.7e-46
Identity = 100/240 (41.67%), Postives = 144/240 (60.00%), Query Frame = 0

Query: 28  CRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDF--------SSRNMAGK 87
           C CI  + F+G L+SDVTC  C   STT DP  DISLDL             S  ++   
Sbjct: 274 CNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSDGSVVNG 333

Query: 88  SAKPADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHI 147
            + P   S  +TL+ CL  FTRPE LGS  K+ C  C   + S KQ+++++LP+V C H+
Sbjct: 334 DSHP---SGATTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKRLPIVACFHL 393

Query: 148 KRFEHSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEI 207
           KRFEHS   +  RKI  Y+ FP  LDM P+++SS  +    N  +    D L+  +K+ +
Sbjct: 394 KRFEHS--AKLRRKITTYVSFPLELDMTPFMASS--KESRMNGQYQQPVDSLNNDNKYSL 453

Query: 208 FAVVSHSGTLESGHYVTYLRL-RNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYK 259
           FAVV+H GTLESGHY T++R  +++W+KCDDA IT+   + V +S+ Y++FY ++   Y+
Sbjct: 454 FAVVNHQGTLESGHYTTFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 506

BLAST of Lcy12g017750 vs. ExPASy Swiss-Prot
Match: P0C8Z3 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Bos taurus OX=9913 GN=USP22 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 7.5e-46
Identity = 100/237 (42.19%), Postives = 145/237 (61.18%), Query Frame = 0

Query: 28  CRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDL--DTQDFSSRNMAGKSA---K 87
           C CI  + F+G L+SDVTC  C   STT DP  DISLDL   +  F   +   +S+    
Sbjct: 282 CNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSESSVVNG 341

Query: 88  PADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRF 147
            +  S  +TL+ CL  FTRPE LGS  K+ C  C   + S KQ++++KLP+V C H+KRF
Sbjct: 342 ESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRF 401

Query: 148 EHSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAV 207
           EHS   +  RKI  Y+ FP  LDM P+++SS  +    N  +    D L+  +K+ +FAV
Sbjct: 402 EHS--AKLRRKITTYVSFPLELDMTPFMASS--KESRMNGQYQQPTDSLNNDNKYSLFAV 461

Query: 208 VSHSGTLESGHYVTYLRL-RNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYK 259
           V+H GTLESGHY +++R  +++W+KCDDA IT+     V +S+ Y++FY ++   Y+
Sbjct: 462 VNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIADVLDSEGYLLFYHKQFLEYE 514

BLAST of Lcy12g017750 vs. ExPASy TrEMBL
Match: A0A5A7VBG6 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001380 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.0e-144
Identity = 247/259 (95.37%), Postives = 257/259 (99.23%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAH+AFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRN+AGKS KP
Sbjct: 294 ENGDCKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKSTKP 353

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
           ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQE RNSVKQMSIR+LPLVLCLHIKRFE
Sbjct: 354 ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFE 413

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSF+RRTSRKIDRYLQFPF+LDMIPYLSSSIIRNR+GNRIFASEGDELDASSKFEIFAVV
Sbjct: 414 HSFIRRTSRKIDRYLQFPFALDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVV 473

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+AN+KSNED+
Sbjct: 474 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIANHKSNEDL 533

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 534 SCLPISPRNDAFQQIAGCC 552

BLAST of Lcy12g017750 vs. ExPASy TrEMBL
Match: A0A1S3BVK6 (Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103493735 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.0e-144
Identity = 247/259 (95.37%), Postives = 257/259 (99.23%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAH+AFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRN+AGKS KP
Sbjct: 294 ENGDCKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKSTKP 353

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
           ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQE RNSVKQMSIR+LPLVLCLHIKRFE
Sbjct: 354 ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFE 413

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSF+RRTSRKIDRYLQFPF+LDMIPYLSSSIIRNR+GNRIFASEGDELDASSKFEIFAVV
Sbjct: 414 HSFIRRTSRKIDRYLQFPFALDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVV 473

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+AN+KSNED+
Sbjct: 474 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIANHKSNEDL 533

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 534 SCLPISPRNDAFQQIAGCC 552

BLAST of Lcy12g017750 vs. ExPASy TrEMBL
Match: A0A6J1FBS8 (Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111442659 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.9e-141
Identity = 244/259 (94.21%), Postives = 252/259 (97.30%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDT DFSSRN+A KS KP
Sbjct: 291 ENGDCKCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTHDFSSRNIASKSMKP 350

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
            D SSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMR+SVKQMSIR+LPLVLCLHIKRFE
Sbjct: 351 VDSSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRSSVKQMSIRRLPLVLCLHIKRFE 410

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDE+DASSKFEIFAVV
Sbjct: 411 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDEIDASSKFEIFAVV 470

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLR+RWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+ NYK NED+
Sbjct: 471 SHSGTLESGHYVTYLRLRSRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIPNYKPNEDL 530

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 531 SCLPISPRNDAFQQIAGCC 549

BLAST of Lcy12g017750 vs. ExPASy TrEMBL
Match: A0A6J1IL13 (Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111477195 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.1e-139
Identity = 241/259 (93.05%), Postives = 250/259 (96.53%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDT DF SRN+A KS KP
Sbjct: 291 ENGDCKCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTHDFLSRNIASKSTKP 350

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
            D SSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMR+SVKQMSIR+LPLVLCLHIKRFE
Sbjct: 351 VDSSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRSSVKQMSIRRLPLVLCLHIKRFE 410

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDE+DASSKFEIFAVV
Sbjct: 411 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDEIDASSKFEIFAVV 470

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLR++WYKCDDAWITEVDEEMVRNSQCYMMFYLQK+ NYK NED+
Sbjct: 471 SHSGTLESGHYVTYLRLRSQWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIPNYKLNEDL 530

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRND FQQIAGCC
Sbjct: 531 SCLPISPRNDTFQQIAGCC 549

BLAST of Lcy12g017750 vs. ExPASy TrEMBL
Match: A0A6J1CA14 (Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111008801 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.1e-136
Identity = 236/259 (91.12%), Postives = 245/259 (94.59%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAHK FSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDT+D +S+N  GKS KP
Sbjct: 299 ENGDCKCIAHKVFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTRDSASKNSTGKSVKP 358

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
           AD SS STLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIR+LPLVLC HIKRFE
Sbjct: 359 ADCSSTSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRRLPLVLCFHIKRFE 418

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELD+SSKFE+FAVV
Sbjct: 419 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDSSSKFEVFAVV 478

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLR RWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+ NYK NED+
Sbjct: 479 SHSGTLESGHYVTYLRLRKRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKVVNYKPNEDL 538

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPIS  NDAF QIAGCC
Sbjct: 539 SCLPISQCNDAFHQIAGCC 557

BLAST of Lcy12g017750 vs. NCBI nr
Match: XP_038897704.1 (ubiquitin C-terminal hydrolase 22 isoform X2 [Benincasa hispida])

HSP 1 Score: 522.7 bits (1345), Expect = 2.0e-144
Identity = 250/259 (96.53%), Postives = 258/259 (99.61%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRN+AGKSAKP
Sbjct: 298 ENGDCKCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKSAKP 357

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
           ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQE RNSVKQMSIR+LPLVLCLHIKRFE
Sbjct: 358 ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFE 417

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV
Sbjct: 418 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 477

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+AN+KSN+++
Sbjct: 478 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIANHKSNDEL 537

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 538 SCLPISPRNDAFQQIAGCC 556

BLAST of Lcy12g017750 vs. NCBI nr
Match: XP_038897703.1 (ubiquitin C-terminal hydrolase 22 isoform X1 [Benincasa hispida])

HSP 1 Score: 522.7 bits (1345), Expect = 2.0e-144
Identity = 250/259 (96.53%), Postives = 258/259 (99.61%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRN+AGKSAKP
Sbjct: 298 ENGDCKCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKSAKP 357

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
           ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQE RNSVKQMSIR+LPLVLCLHIKRFE
Sbjct: 358 ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFE 417

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV
Sbjct: 418 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 477

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+AN+KSN+++
Sbjct: 478 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIANHKSNDEL 537

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 538 SCLPISPRNDAFQQIAGCC 556

BLAST of Lcy12g017750 vs. NCBI nr
Match: XP_008452825.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22 [Cucumis melo] >KAA0064570.1 ubiquitin carboxyl-terminal hydrolase 22 [Cucumis melo var. makuwa] >TYK20020.1 ubiquitin carboxyl-terminal hydrolase 22 [Cucumis melo var. makuwa])

HSP 1 Score: 519.6 bits (1337), Expect = 1.7e-143
Identity = 247/259 (95.37%), Postives = 257/259 (99.23%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAH+AFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRN+AGKS KP
Sbjct: 294 ENGDCKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKSTKP 353

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
           ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQE RNSVKQMSIR+LPLVLCLHIKRFE
Sbjct: 354 ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFE 413

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSF+RRTSRKIDRYLQFPF+LDMIPYLSSSIIRNR+GNRIFASEGDELDASSKFEIFAVV
Sbjct: 414 HSFIRRTSRKIDRYLQFPFALDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVV 473

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+AN+KSNED+
Sbjct: 474 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIANHKSNEDL 533

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 534 SCLPISPRNDAFQQIAGCC 552

BLAST of Lcy12g017750 vs. NCBI nr
Match: XP_011654218.1 (ubiquitin C-terminal hydrolase 22 [Cucumis sativus] >KAE8649935.1 hypothetical protein Csa_012161 [Cucumis sativus])

HSP 1 Score: 515.8 bits (1327), Expect = 2.4e-142
Identity = 246/259 (94.98%), Postives = 255/259 (98.46%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAH+AFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRN+AGK  KP
Sbjct: 294 ENGDCKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKP 353

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
           ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQE RNSVKQMSIR+LPLVLCLHIKRFE
Sbjct: 354 ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFE 413

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSF+RRTSRKIDRYLQFPFSLDMIPYLSSSIIRNR+GNRIFASEGDELDASSKFEIFAVV
Sbjct: 414 HSFIRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVV 473

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK A++KSNED+
Sbjct: 474 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDL 533

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 534 SCLPISPRNDAFQQIAGCC 552

BLAST of Lcy12g017750 vs. NCBI nr
Match: XP_022935893.1 (ubiquitin carboxyl-terminal hydrolase 22 [Cucurbita moschata])

HSP 1 Score: 510.4 bits (1313), Expect = 1.0e-140
Identity = 244/259 (94.21%), Postives = 252/259 (97.30%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKP 83
           ENGDC+CIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDT DFSSRN+A KS KP
Sbjct: 291 ENGDCKCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTHDFSSRNIASKSMKP 350

Query: 84  ADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFE 143
            D SSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMR+SVKQMSIR+LPLVLCLHIKRFE
Sbjct: 351 VDSSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRSSVKQMSIRRLPLVLCLHIKRFE 410

Query: 144 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVV 203
           HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDE+DASSKFEIFAVV
Sbjct: 411 HSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDEIDASSKFEIFAVV 470

Query: 204 SHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSNEDV 263
           SHSGTLESGHYVTYLRLR+RWYKCDDAWITEVDEEMVRNSQCYMMFYLQK+ NYK NED+
Sbjct: 471 SHSGTLESGHYVTYLRLRSRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIPNYKPNEDL 530

Query: 264 SCLPISPRNDAFQQIAGCC 283
           SCLPISPRNDAFQQIAGCC
Sbjct: 531 SCLPISPRNDAFQQIAGCC 549

BLAST of Lcy12g017750 vs. TAIR 10
Match: AT5G10790.1 (ubiquitin-specific protease 22 )

HSP 1 Score: 311.6 bits (797), Expect = 6.4e-85
Identity = 162/262 (61.83%), Postives = 195/262 (74.43%), Query Frame = 0

Query: 24  ENGDCRCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGK---S 83
           +N +C+CI HKAFSG LRSDVTC TCG TSTTYDP +DISL LD+ +  S     K   S
Sbjct: 292 DNEECQCITHKAFSGLLRSDVTCTTCGSTSTTYDPFIDISLTLDSMNGFSPADCRKNRYS 351

Query: 84  AKPADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIK 143
             P+  +   TLSGCLD FTR EKLG DQKL CQ+C E R S KQMSIR+LPL+LCLH+K
Sbjct: 352 GGPSVNAIMPTLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVK 411

Query: 144 RFEHSFVRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGD-ELDASS---- 203
           RFEHS  R+TSRKID YLQ+PF L+M PYLSSSII  R+GNRIFA +G+ E D+SS    
Sbjct: 412 RFEHSLTRKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSP 471

Query: 204 --KFEIFAVVSHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK 263
             +FEIFAVV+H G LESGHYVTYLRL+  WY+CDDAWI EV+EE+VR  +CYM+FY Q+
Sbjct: 472 SAEFEIFAVVTHKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQE 531

Query: 264 MANYKSNEDVSCLPISPRNDAF 276
               K+++++S   IS   DAF
Sbjct: 532 TVIQKAHKELSYQVIS-MADAF 552

BLAST of Lcy12g017750 vs. TAIR 10
Match: AT4G17895.1 (ubiquitin-specific protease 20 )

HSP 1 Score: 115.2 bits (287), Expect = 8.8e-26
Identity = 72/227 (31.72%), Postives = 121/227 (53.30%), Query Frame = 0

Query: 36  FSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKPADFSSKSTLSGC 95
           FSG+L S + C  C + S TY+  V +SL+++  D                    TL   
Sbjct: 296 FSGRLISGLRCCNCDYVSETYEKSVGLSLEIEDVD--------------------TLGSA 355

Query: 96  LDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHIKRFEHSFVRRTSRKID 155
           L+ FTR EKL  D++L C NC E  +  KQ+ + KLPLV   H+KRF+++ +     KI 
Sbjct: 356 LESFTRVEKL--DEQLTCDNCNEKVSKEKQLLLDKLPLVATFHLKRFKNNGL--YMEKIY 415

Query: 156 RYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVVSHSG-TLESGHY 215
           ++++ P  +D+ PY+     RN   N +          S+K+ ++A+V H G ++  GHY
Sbjct: 416 KHVKIPLEIDLQPYM-----RNIQENEV----------STKYHLYALVEHFGYSVAYGHY 475

Query: 216 VTYLRLRNR-WYKCDDAWITEVDEEMVRNSQCYMMFYLQKMANYKSN 261
            +Y+R   + W+  DD+ +T +DE+MV +   Y++FY ++   + S+
Sbjct: 476 SSYVRSAPKIWHHFDDSKVTRIDEDMVLSQDSYILFYAREGTRWFSS 483

BLAST of Lcy12g017750 vs. TAIR 10
Match: AT5G57990.1 (ubiquitin-specific protease 23 )

HSP 1 Score: 113.6 bits (283), Expect = 2.6e-25
Identity = 71/231 (30.74%), Postives = 111/231 (48.05%), Query Frame = 0

Query: 29  RCIAHKAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNMAGKSAKPADFSS 88
           R + HK F G LRS V C  C   S  +DP +D+SLD+   D                  
Sbjct: 224 RSLVHKIFGGSLRSQVKCEQCSHCSNKFDPFLDLSLDISKAD------------------ 283

Query: 89  KSTLSGCLDLFTRPEKLGSDQKLF-CQNCQEMRNSVKQMSIRKLPLVLCLHIKRFEHSFV 148
             +L   L  FT  E L +  K++ C+ C++   + KQ+++ K P VL +H+KRFE    
Sbjct: 284 --SLQRALSRFTAVELLDNGAKVYQCERCKQKVKAKKQLTVSKAPYVLTVHLKRFE---- 343

Query: 149 RRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFEIFAVVSHSG 208
              S KIDR + F  ++DM P++S                    + + K+ ++ V+ H G
Sbjct: 344 AHRSEKIDRKVDFTSAIDMKPFVSG-----------------PHEGNLKYTLYGVLVHYG 403

Query: 209 -TLESGHYVTYLRLRN-RWYKCDDAWITEVDEEMVRNSQCYMMFYLQKMAN 257
            +  SGHY  ++R  +  WY  DD  + +V E+ V N + YM+FY++   N
Sbjct: 404 RSSHSGHYACFVRTSSGMWYSLDDNRVVQVSEKTVFNQKAYMLFYVRDRQN 413

BLAST of Lcy12g017750 vs. TAIR 10
Match: AT4G10570.1 (ubiquitin-specific protease 9 )

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-23
Identity = 68/197 (34.52%), Postives = 102/197 (51.78%), Query Frame = 0

Query: 86  FSSKST------LSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHI 145
           FS+K T      L  CL+ F   E LG D   FC +C+E R + K++ + KLP +L  H+
Sbjct: 738 FSAKKTRQESISLFSCLEAFLAEEPLGPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHL 797

Query: 146 KRFEHSFVRRTSRKIDRYLQFP-FSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFE 205
           KRF +S  R    KID ++ FP   LD+  Y     ++N+ G             S  +E
Sbjct: 798 KRFTYS--RYLKNKIDTFVNFPVHDLDLSKY-----VKNKNGQ------------SYLYE 857

Query: 206 IFAVVSHSGTLESGHYVTYLRL--RNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQ---- 265
           ++AV +H G L  GHY  Y +L   N+WY  DD+ ++ V+E  +RNS  Y++FY +    
Sbjct: 858 LYAVSNHYGGLGGGHYTAYAKLIDDNKWYHFDDSHVSSVNESEIRNSAAYVLFYRRVRSE 915

Query: 266 ---KMANYKSNEDVSCL 267
              + A   ++ D SCL
Sbjct: 918 TETQTAEMSTDMDYSCL 915

BLAST of Lcy12g017750 vs. TAIR 10
Match: AT4G10590.2 (ubiquitin-specific protease 10 )

HSP 1 Score: 103.2 bits (256), Expect = 3.5e-22
Identity = 61/174 (35.06%), Postives = 91/174 (52.30%), Query Frame = 0

Query: 86  FSSKST------LSGCLDLFTRPEKLGSDQKLFCQNCQEMRNSVKQMSIRKLPLVLCLHI 145
           FS+K T      L  CL+ F   E LG D   FC +C+E R + K++ + KLP +L  H+
Sbjct: 737 FSAKKTRQESISLFSCLEAFLAEEPLGPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHL 796

Query: 146 KRFEHSFVRRTSRKIDRYLQFP-FSLDMIPYLSSSIIRNRYGNRIFASEGDELDASSKFE 205
           KRF +S  R    KID ++ FP   LD+  Y+                  ++ D S  +E
Sbjct: 797 KRFTYS--RYLKNKIDTFVNFPVHDLDLSKYVK-----------------NKNDQSYLYE 856

Query: 206 IFAVVSHSGTLESGHYVTYLRL--RNRWYKCDDAWITEVDEEMVRNSQCYMMFY 251
           ++AV +H G L  GHY  Y +L   N WY  DD+ ++ V+E  ++NS  Y++FY
Sbjct: 857 LYAVSNHYGGLGGGHYTAYAKLIDDNEWYHFDDSHVSSVNESEIKNSAAYVLFY 891

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LEW09.0e-8461.83Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 ... [more]
Q6DCJ15.2e-4742.62Ubiquitin carboxyl-terminal hydrolase 22-B OS=Xenopus laevis OX=8355 GN=usp22-b ... [more]
Q6GNI66.7e-4742.62Ubiquitin carboxyl-terminal hydrolase 22-A OS=Xenopus laevis OX=8355 GN=usp22-a ... [more]
A6H8I05.7e-4641.67Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV... [more]
P0C8Z37.5e-4642.19Ubiquitin carboxyl-terminal hydrolase 22 OS=Bos taurus OX=9913 GN=USP22 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A5A7VBG68.0e-14495.37Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3BVK68.0e-14495.37Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103493735 PE=3 SV=1[more]
A0A6J1FBS84.9e-14194.21Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111442659 PE=3 SV=1[more]
A0A6J1IL132.1e-13993.05Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111477195 PE=3 SV=1[more]
A0A6J1CA142.1e-13691.12Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111008801 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038897704.12.0e-14496.53ubiquitin C-terminal hydrolase 22 isoform X2 [Benincasa hispida][more]
XP_038897703.12.0e-14496.53ubiquitin C-terminal hydrolase 22 isoform X1 [Benincasa hispida][more]
XP_008452825.11.7e-14395.37PREDICTED: ubiquitin carboxyl-terminal hydrolase 22 [Cucumis melo] >KAA0064570.1... [more]
XP_011654218.12.4e-14294.98ubiquitin C-terminal hydrolase 22 [Cucumis sativus] >KAE8649935.1 hypothetical p... [more]
XP_022935893.11.0e-14094.21ubiquitin carboxyl-terminal hydrolase 22 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G10790.16.4e-8561.83ubiquitin-specific protease 22 [more]
AT4G17895.18.8e-2631.72ubiquitin-specific protease 20 [more]
AT5G57990.12.6e-2530.74ubiquitin-specific protease 23 [more]
AT4G10570.11.8e-2334.52ubiquitin-specific protease 9 [more]
AT4G10590.23.5e-2235.06ubiquitin-specific protease 10 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 20..256
e-value: 6.0E-62
score: 211.7
NoneNo IPR availablePANTHERPTHR21646:SF49UBIQUITIN C-TERMINAL HYDROLASE 22coord: 23..277
NoneNo IPR availablePANTHERPTHR21646UBIQUITIN CARBOXYL-TERMINAL HYDROLASEcoord: 23..277
IPR001394Peptidase C19, ubiquitin carboxyl-terminal hydrolasePFAMPF00443UCHcoord: 28..250
e-value: 5.0E-27
score: 95.1
IPR028889Ubiquitin specific protease domainPROSITEPS50235USP_3coord: 1..253
score: 34.939018
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 25..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g017750.1Lcy12g017750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004843 thiol-dependent deubiquitinase
molecular_function GO:0008270 zinc ion binding