Lcy12g017460 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g017460 vs. ExPASy Swiss-Prot
Match: Q9SZ58 (Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT1 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 4.2e-115
Identity = 228/461 (49.46%), Postives = 317/461 (68.76%), Query Frame = 0

Query: 9   SQVSLTKTLTVFPKTLN--PPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTK 68
           +++ + + L V+P+  N    KL+ LSNLDRQCPL MY +FFY  +   +   FDS+F+ 
Sbjct: 7   TRIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNT---TTRDFDSVFSN 66

Query: 69  LKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAI-TNVKVSELGDLSNYNE 128
           LK+GLEET+S+W PAAGRL L     KLN+RC++ GA++V+A+ T VK+SELGDL+ YNE
Sbjct: 67  LKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQYNE 126

Query: 129 FFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAAN 188
           F+E LVYKP L+G+FS  PLVVA QVTRF CGGYSIG G SHSLFDG +AY+F+ AWA+N
Sbjct: 127 FYENLVYKPSLDGDFSVMPLVVA-QVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWASN 186

Query: 189 SAISKDNKGQYGAQ----LHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAM 248
           S I   +  +   +    + +PVH+R  LL+ + A       + A AI HLYQLI +  M
Sbjct: 187 SHIHNKSNSKITNKKEDVVIKPVHDRRNLLVNRDA----VRETNAAAICHLYQLIKQAMM 246

Query: 249 ENEE-----EMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTK 308
             +E     E+    +V +TF ++   IE +KKK+L     F CSSF+ +AAHLWKART+
Sbjct: 247 TYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSL---EGFMCSSFEFLAAHLWKARTR 306

Query: 309 AVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQ-LEEESYKTIIEMI 368
           A+ + ++ MVCLQFAVD R +   PLP+GFSGNA+VLASVA TA++ LEE + ++I+  I
Sbjct: 307 ALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELTLESIVNKI 366

Query: 369 RNAKSRVDNDYINAYKQGLEGP-QASLPPLKEHTVVSDWTRMPFHKIEFLHG--EAAYAS 428
           R AK  +D  YIN+Y + L G    +LPPLKE T++SDWT+MPFH + F +G   A Y +
Sbjct: 367 REAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNGGEPADYMA 426

Query: 429 PLKPPILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFL 454
           PL PP+ +VAYF+++P D++ + VR+ L    ++ FS++FL
Sbjct: 427 PLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHFL 456

BLAST of Lcy12g017460 vs. ExPASy Swiss-Prot
Match: Q94CD1 (Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN=HHT1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.8e-42
Identity = 132/454 (29.07%), Postives = 220/454 (48.46%), Query Frame = 0

Query: 8   HSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTKL 67
           H +V   +   V P++     L  LSNLD+   + +  I+ +      +  +       +
Sbjct: 30  HLEVHQKEPALVKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQ----VI 89

Query: 68  KIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDLSNYN-EF 127
           K  L + L  + P AGRL +SP +GKL + C   G + V+A  N K+ E+GD++  + E 
Sbjct: 90  KKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPET 149

Query: 128 FEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANS 187
             KLVY  V   N  E P V A QVT+F CGG+ +G  ++H +FDG  A +F+++W   +
Sbjct: 150 LGKLVYDVVDAKNILEIPPVTA-QVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVA 209

Query: 188 AISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEE 247
                     G  L  P     T+L  +              I +L+Q   E  +E++  
Sbjct: 210 R---------GLPLTTPPFSDRTILNARNPP----------KIENLHQEFEE--IEDKSN 269

Query: 248 MG----KKEYVCRTFRVSRGMIERLKKKALGENGAF---ACSSFDVIAAHLWKARTKAVS 307
           +     K+  + R+F      I++LK +A   + +    +C+SF+ ++A +W+ARTK++ 
Sbjct: 270 INSLYTKEPTLYRSFCFDPEKIKKLKLQATENSESLLGNSCTSFEALSAFVWRARTKSLK 329

Query: 308 VSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAK 367
           +  ++   L FAVD R K  P LPKG+ GN  VL +    A +L E+     + ++R A 
Sbjct: 330 MLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAI 389

Query: 368 SRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKPPIL 427
             V + Y+ +     E  +A  P L    +++ W+R+ FH  +F  GE   + P+  P  
Sbjct: 390 KMVTDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEK 449

Query: 428 EVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFL 454
           EV  FL      ++I+V + L +  +D F   FL
Sbjct: 450 EVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFL 455

BLAST of Lcy12g017460 vs. ExPASy Swiss-Prot
Match: Q9FFQ7 (Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FACT PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.4e-38
Identity = 122/441 (27.66%), Postives = 209/441 (47.39%), Query Frame = 0

Query: 11  VSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTKLKIG 70
           V+  + + V P +  P  L  LSNLD+   + +   +++  +S  +  S++     +K  
Sbjct: 9   VTRKEPVLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNSRSNEESYE----VIKKS 68

Query: 71  LEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNV---KVSELGDLSNYNEFF 130
           L E L  + PAAGRL +SP +GK+ + C   G ++V+A  N    K+ +     +  E  
Sbjct: 69  LSEVLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETL 128

Query: 131 EKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANSA 190
           EKLVY      N  E P VV  QVT F CGG+ +G G++H++FDG AA +FL++WA  + 
Sbjct: 129 EKLVYDVPGARNILEIPPVVV-QVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETAR 188

Query: 191 ISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEEM 250
                    G  L  P     TLL  +            +   H     +E      +  
Sbjct: 189 ---------GLPLSVPPFLDRTLLRPR--------TPPKIEFPHNEFEDLEDISGTGKLY 248

Query: 251 GKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTKAVSVSKEKMVC 310
             ++ V ++F      +ERLK  A   +     ++F  +   LW+AR +A+ +  ++ + 
Sbjct: 249 SDEKLVYKSFLFGPEKLERLKIMAETRS-----TTFQTLTGFLWRARCQALGLKPDQRIK 308

Query: 311 LQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAKSRVDNDYI 370
           L FA D R++ +P LPKG+SGN  V      TA ++        + +++ A   V++ ++
Sbjct: 309 LLFAADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDGFM 368

Query: 371 NAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKPPILEVAYFLQS 430
            +     E  +A  P L    +++ W ++ FH  +F  GE   + P+  P  EV  FL  
Sbjct: 369 RSAIDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFLPC 420

Query: 431 PTDSQAIDVRVPLLSHTLDAF 449
            +D+++I+V + L    +  F
Sbjct: 429 GSDTKSINVLLGLPGSAMKVF 420

BLAST of Lcy12g017460 vs. ExPASy Swiss-Prot
Match: Q8S9G6 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=29808 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.9e-31
Identity = 113/410 (27.56%), Postives = 188/410 (45.85%), Query Frame = 0

Query: 11  VSLTKTLTVFPKTLNPPKLLELSNLDRQCPLH---MYLIFFYNPSSVPSNSSFDSLFTKL 70
           V+L + + V P    P   L+LS++D    +       +  YN S  P+  S D     +
Sbjct: 8   VNLNEKVMVGPSLPLPKTTLQLSSIDNLPGVRGSIFNALLIYNASPSPTMVSADPA-KLI 67

Query: 71  KIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDLSNYNEFF 130
           +  L + L  +PP AGRL     +G L + C   GA+ ++A+ + ++S LGD  + N  F
Sbjct: 68  REALAKILVYYPPFAGRLR-ETENGDLEVECTGEGAMFLEAMADNELSVLGDFDDSNPSF 127

Query: 131 EKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANSA 190
           ++L++   L+ NF + PL+V  QVTRF CGG+ +G    H + DG  A  FL   A  + 
Sbjct: 128 QQLLFSLPLDTNFKDLPLLVV-QVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLAEMA- 187

Query: 191 ISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEEM 250
                +G+    L  P+  R  + +     +              +     RA    E++
Sbjct: 188 -----RGEVKLSL-EPIWNRELVKLDDPKYL------------QFFHFEFLRAPSIVEKI 247

Query: 251 GKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTKAVSVSKEKMVC 310
                V   F +    I  +K+  + E   F CSSF+V +A  W ART+A  + + + V 
Sbjct: 248 -----VQTYFIIDFETINYIKQSVMEECKEF-CSSFEVASAMTWIARTRAFQIPESEYVK 307

Query: 311 LQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAKSRVDNDYI 370
           + F +D RN   PPLP G+ GN+   A      + L   S    I +I+ +K  +++   
Sbjct: 308 ILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKVSLND--- 367

Query: 371 NAYKQGLEGPQASLPPLKEHTVV--SDWTRMPFHKIEFLHGEAAYASPLK 416
           N   + +  P      +    VV  +DW+R+ F +++F  G A   SP++
Sbjct: 368 NFKSRAVVKPSELDVNMNHENVVAFADWSRLGFDEVDFGWGNAVSVSPVQ 386

BLAST of Lcy12g017460 vs. ExPASy Swiss-Prot
Match: Q9M6F0 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus cuspidata OX=99806 GN=TAT PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.4e-30
Identity = 112/410 (27.32%), Postives = 187/410 (45.61%), Query Frame = 0

Query: 11  VSLTKTLTVFPKTLNPPKLLELSNLDRQCPLH---MYLIFFYNPSSVPSNSSFDSLFTKL 70
           V+L + + V P    P   L+LS++D    +       +  YN S  P+  S D     +
Sbjct: 8   VNLIEKVMVGPSPPLPKTTLQLSSIDNLPGVRGSIFNALLIYNASPSPTMISADPA-KPI 67

Query: 71  KIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDLSNYNEFF 130
           +  L + L  +PP AGRL     +G L + C   GA+ ++A+ + ++S LGD  + N  F
Sbjct: 68  REALAKILVYYPPFAGRLR-ETENGDLEVECTGEGAMFLEAMADNELSVLGDFDDSNPSF 127

Query: 131 EKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANSA 190
           ++L++   L+ NF +  L+V  QVTRF CGG+ +G    H + DG  A  FL   A  + 
Sbjct: 128 QQLLFSLPLDTNFKDLSLLVV-QVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLAEMA- 187

Query: 191 ISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEEM 250
                +G+    L  P+  R  + +     +              +     RA    E++
Sbjct: 188 -----RGEVKLSL-EPIWNRELVKLDDPKYL------------QFFHFEFLRAPSIVEKI 247

Query: 251 GKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTKAVSVSKEKMVC 310
                V   F +    I  +K+  + E   F CSSF+V +A  W ART+A  + + + V 
Sbjct: 248 -----VQTYFIIDFETINYIKQSVMEECKEF-CSSFEVASAMTWIARTRAFQIPESEYVK 307

Query: 311 LQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAKSRVDNDYI 370
           + F +D RN   PPLP G+ GN+   A      + L   S    I +I+ +K  +++   
Sbjct: 308 ILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKVSLND--- 367

Query: 371 NAYKQGLEGPQASLPPLKEHTVV--SDWTRMPFHKIEFLHGEAAYASPLK 416
           N   + +  P      +    VV  +DW+R+ F +++F  G A   SP++
Sbjct: 368 NFKSRAVVKPSELDVNMNHENVVAFADWSRLGFDEVDFGWGNAVSVSPVQ 386

BLAST of Lcy12g017460 vs. ExPASy TrEMBL
Match: A0A6J1FK90 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446510 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 3.3e-211
Identity = 385/461 (83.51%), Postives = 416/461 (90.24%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MATSH     VS+TKTLTVFPKTLN PKLLELSNLDRQCPLH+YLIFFY PS   S+SSF
Sbjct: 1   MATSH-----VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSF 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           DS+FTKLKIGLEETLS+W PAAGRLE +PA+GKLNLRC+NRGAILVQ+ TNV++S+LGDL
Sbjct: 61  DSVFTKLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNVEISKLGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFEKLVYKPVLNGNFSEFPLVVA QVTRFGCGGYSIGTGISHSLFDGPAAY+FLS
Sbjct: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVA-QVTRFGCGGYSIGTGISHSLFDGPAAYEFLS 180

Query: 181 AWAANSAISKDNKGQYGAQLHRPVHERGTLLMG---KRAAVASA-SVSGAVAIGHLYQLI 240
           AWAA SAI KD K  YG +LH PVHERG LLMG   KR+    A  VSGAVAI HLY+LI
Sbjct: 181 AWAAKSAIFKDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMPVSGAVAIDHLYRLI 240

Query: 241 MERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTK 300
           M+ +M  +EEMGKKEYV RTFR+SRGMIERLKK A+GENG FACSSFDVIAAHLWKARTK
Sbjct: 241 MQ-SMTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTK 300

Query: 301 AVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIR 360
           A+SVSKEKMVCLQFAV+ARNKL+PPLPKGFSGNAFVLASVALTA+QLE+ESYKTI++MIR
Sbjct: 301 AMSVSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIR 360

Query: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKP 420
           NAKSRVDNDYINAYKQGLEGPQASLPPLKE TVVSDWTRMPFHKIEFLHG+A YASPLKP
Sbjct: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKP 420

Query: 421 PILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           PIL+VAYFLQSP DSQAIDVRVPLL  TL+AFSSYFL  MQ
Sbjct: 421 PILDVAYFLQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of Lcy12g017460 vs. ExPASy TrEMBL
Match: A0A6J1IT61 (brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC111480377 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.4e-209
Identity = 382/463 (82.51%), Postives = 414/463 (89.42%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MATSH     VS+TKTLTVFPKTLN PKLLELSNLDRQCPLH+YLIFFY PS   S+SS 
Sbjct: 1   MATSH-----VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSL 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           DS+FT LKIGLEETLS+W PAAGRLE +PA+GKLNLRC+NRGAILVQ+ TN+++SELGDL
Sbjct: 61  DSIFTNLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFEKLVYKPVLNGNFSEFPLVVA QVTRFGCGGYSIGTGISHSLFDGPAAY+FLS
Sbjct: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVA-QVTRFGCGGYSIGTGISHSLFDGPAAYEFLS 180

Query: 181 AWAANSAISKDNKGQYGAQLHRPVHERGTLLM---GKRAAVASA---SVSGAVAIGHLYQ 240
           AWAA SAI KD K  YG +LH PVHERG LLM    KR++   A   SVSGAVAI HLY+
Sbjct: 181 AWAAKSAIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYR 240

Query: 241 LIMERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKAR 300
           LIM+ ++  +EEMGKKEYV RTFR+SRGMIERLKK A+GENG FACSSFDVIAAHLWKAR
Sbjct: 241 LIMQ-SVTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKAR 300

Query: 301 TKAVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEM 360
           TKA+SVS EKMVCLQFAV+ARNKL+PPLPKGFSGNAFVLASVALTA+QLE+ESYKTI+EM
Sbjct: 301 TKAMSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEM 360

Query: 361 IRNAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPL 420
           IRNAKSRVDNDYINAYKQGLEGPQASLPPLKE TVVSDWTRMPFHKIEFLHG+A YASPL
Sbjct: 361 IRNAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPL 420

Query: 421 KPPILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           KPPIL+VAYFLQSP DSQAIDVRVPLL  TL+AFSSYFL  MQ
Sbjct: 421 KPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 453

BLAST of Lcy12g017460 vs. ExPASy TrEMBL
Match: A0A6J1F9T5 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443397 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 8.3e-207
Identity = 374/462 (80.95%), Postives = 409/462 (88.53%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MAT H     VSLTK+LTVFPKTLNP KLLELSNLDRQCPLHMYLIFFYNPSS+ +NSS 
Sbjct: 1   MATPH-----VSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSL 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           DS+FTKLKIGLEETLS+W PAAGRLE +PADGKLNLRCDNRGAIL +A+T+VK+SELGDL
Sbjct: 61  DSVFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFE+LV+KPVL+G+F EFPLVVA QVTRFGCGGYSIGTGISHSLFDGPAAY+FLS
Sbjct: 121 SNYNEFFEELVHKPVLDGDFCEFPLVVA-QVTRFGCGGYSIGTGISHSLFDGPAAYEFLS 180

Query: 181 AWAANSAISKDNKGQYGAQLHRPVHERGTLLMG------KRAAVASASVSGAVAIGHLYQ 240
           AWA  SAI KD+K QYG +L +PVHERG LLMG           A+ S +GAVAI HLY+
Sbjct: 181 AWATKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYK 240

Query: 241 LIMERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKAR 300
           LIM+ AM N EEM KKEY CRT RVSRGMIE LKKKA GENG F CSSFDVIAAHLWKAR
Sbjct: 241 LIMQSAMGN-EEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKAR 300

Query: 301 TKAVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEM 360
           TKA+SVSKEK+VCLQFAVDARNKL PPLPKGFSGN+FVLASVAL AKQLEEESYKTIIEM
Sbjct: 301 TKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEM 360

Query: 361 IRNAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPL 420
           IR+AKS+VD+DY+NAY+ GL+GPQASLPPLKE TV+SDWTRMPFHKIEFLHGEAAYASPL
Sbjct: 361 IRDAKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPL 420

Query: 421 KPPILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASM 457
           KPPIL+VAYFLQ+PTDSQAIDVRV LLS TLDAFS+YF  SM
Sbjct: 421 KPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 455

BLAST of Lcy12g017460 vs. ExPASy TrEMBL
Match: A0A0A0L0F4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652090 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.2e-205
Identity = 374/453 (82.56%), Postives = 405/453 (89.40%), Query Frame = 0

Query: 7   SHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTK 66
           ++ QVSLTK+LTVFPKT NP K +ELSNLDRQCPLHMYLIFFYNPSS+ +NSS D +F K
Sbjct: 2   ANPQVSLTKSLTVFPKTPNPTKFVELSNLDRQCPLHMYLIFFYNPSSIYTNSSPDLVFNK 61

Query: 67  LKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDLSNYNEF 126
           LKIGLEETLSIW PAAGRL+ +P DGKLNLRCDNRGAILVQAITNVK+SELGDLSNYNEF
Sbjct: 62  LKIGLEETLSIWSPAAGRLDRNPTDGKLNLRCDNRGAILVQAITNVKMSELGDLSNYNEF 121

Query: 127 FEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANS 186
           FEKLV+KP+LNGNFSEFPLVVA QVTRFGCGGY+IGTGISHSLFDGPAAYDFLSAWAANS
Sbjct: 122 FEKLVHKPILNGNFSEFPLVVA-QVTRFGCGGYAIGTGISHSLFDGPAAYDFLSAWAANS 181

Query: 187 AISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEE 246
           AI KDN      Q   P+HERG LL G  A   S +V+ A AI HLY+LIM+ AM N EE
Sbjct: 182 AIFKDNNKM---QTITPIHERGRLLKGTVANRCSRTVAVAAAIDHLYKLIMQSAMGN-EE 241

Query: 247 MGKKEYVCRTFRVSRGMIERLKKKALGE-NG-AFACSSFDVIAAHLWKARTKAVSVSKEK 306
           +G+KEYVCRTFRVS+ MIERLK  ALGE NG AF+CSSFD+IAAHLWKARTKA+SVSKEK
Sbjct: 242 VGRKEYVCRTFRVSKEMIERLKMNALGETNGAAFSCSSFDLIAAHLWKARTKALSVSKEK 301

Query: 307 MVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAKSRVDN 366
           MVCLQFAVDARNKL+PPLPKGFSGNAFVLASVALTAKQL+EESYKTIIEMI+NAKSRVDN
Sbjct: 302 MVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLDEESYKTIIEMIQNAKSRVDN 361

Query: 367 DYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKPPILEVAYF 426
           DYINAY+QGLEGPQASLPPLKE TVVSDWTRMPFHKI+FLHGEAAYASPLKPP+LEVAYF
Sbjct: 362 DYINAYQQGLEGPQASLPPLKELTVVSDWTRMPFHKIQFLHGEAAYASPLKPPVLEVAYF 421

Query: 427 LQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           LQ+PTD QAIDV VPLL HT DAFSSYFL  MQ
Sbjct: 422 LQNPTDGQAIDVHVPLLEHTFDAFSSYFLTRMQ 449

BLAST of Lcy12g017460 vs. ExPASy TrEMBL
Match: A0A1S3BW29 (omega-hydroxypalmitate O-feruloyl transferase OS=Cucumis melo OX=3656 GN=LOC103493765 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.9e-205
Identity = 373/453 (82.34%), Postives = 399/453 (88.08%), Query Frame = 0

Query: 7   SHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTK 66
           ++ QVSLTK+L V PKT NPPK LELSNLDRQCPLHMYLIFFYNPSS+ +NSS D +F K
Sbjct: 2   ANPQVSLTKSLIVIPKTSNPPKFLELSNLDRQCPLHMYLIFFYNPSSICTNSSLDLVFNK 61

Query: 67  LKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDLSNYNEF 126
           LKIGLEETLSIW PAAGRL+ +P DGKLNLRCDNRGAILVQAITNVK+SELGDLSNYN+F
Sbjct: 62  LKIGLEETLSIWSPAAGRLDRNPTDGKLNLRCDNRGAILVQAITNVKISELGDLSNYNDF 121

Query: 127 FEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANS 186
           FEKLV KPVLNGNFSEFPLVVA QVTRFGCGGY+IGTGISHSLFDGPAAYDFLSAWA NS
Sbjct: 122 FEKLVQKPVLNGNFSEFPLVVA-QVTRFGCGGYAIGTGISHSLFDGPAAYDFLSAWATNS 181

Query: 187 AISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEE 246
           AI KD+   Y  Q   PVHERG LL G      +   SG VAI HLY+LIM+ AM N EE
Sbjct: 182 AIFKDD--NYKMQTISPVHERGRLLTG----TVANRCSGTVAIDHLYKLIMQSAMGN-EE 241

Query: 247 MGKKEYVCRTFRVSRGMIERLKKKALGE--NGAFACSSFDVIAAHLWKARTKAVSVSKEK 306
           +G+KEYVCRTFRVS+ MIERLK  ALGE   GAFACSSFD+IAAHLWKARTKA+SVSKEK
Sbjct: 242 VGRKEYVCRTFRVSKEMIERLKMNALGEINGGAFACSSFDLIAAHLWKARTKALSVSKEK 301

Query: 307 MVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAKSRVDN 366
           MVCLQFAVDARNKL+PPLPKGFSGNAFVLASVALTAKQL+EESYKTIIEMI+ AKSRVDN
Sbjct: 302 MVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLDEESYKTIIEMIQKAKSRVDN 361

Query: 367 DYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKPPILEVAYF 426
           DYINAY+QGLEGPQASLPPLKE TVVSDWTRMPFHKI+FLHGEAAYASPLKPPILEVAYF
Sbjct: 362 DYINAYQQGLEGPQASLPPLKELTVVSDWTRMPFHKIQFLHGEAAYASPLKPPILEVAYF 421

Query: 427 LQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           LQ+PTD QAIDV VPLL HT DAFSSYFL  MQ
Sbjct: 422 LQNPTDGQAIDVHVPLLEHTFDAFSSYFLTRMQ 446

BLAST of Lcy12g017460 vs. NCBI nr
Match: XP_038898484.1 (brassinosteroid-related acyltransferase 1 [Benincasa hispida])

HSP 1 Score: 763.5 bits (1970), Expect = 1.1e-216
Identity = 398/466 (85.41%), Postives = 420/466 (90.13%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MAT HESHSQVSLTK LTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSV SNSS 
Sbjct: 1   MATPHESHSQVSLTKFLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVYSNSSL 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           D +FTKLKIGLE+TLSIW PAAGRL+ +  DGKLNLRCDNRGAILVQAITNVK+SELGDL
Sbjct: 61  DLVFTKLKIGLEQTLSIWFPAAGRLDRNSTDGKLNLRCDNRGAILVQAITNVKLSELGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFE LV+KPVLNGNFSEFPLVVA QVTRFGCGGY+IGTGISHSLFDGPAAYDFLS
Sbjct: 121 SNYNEFFENLVHKPVLNGNFSEFPLVVA-QVTRFGCGGYAIGTGISHSLFDGPAAYDFLS 180

Query: 181 AWAANSAISKDNKGQYGAQ-LHR---PVHERGTLLMGKRAAVASASVSG--AVAIGHLYQ 240
           AWAANSAI KD+  QYG + LHR   P+HERG LL G  A   S S +G  AVAI HLY+
Sbjct: 181 AWAANSAIFKDH--QYGGELLHRIISPMHERGRLLTGSVANRCSRSGTGTVAVAIDHLYK 240

Query: 241 LIMERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENG---AFACSSFDVIAAHLW 300
           LIM+ AM NEEE+GKKEYVC+TFRV+RGMIERLKK ALGENG   AFACSSFDVIAAHLW
Sbjct: 241 LIMQSAMGNEEEVGKKEYVCKTFRVNRGMIERLKKNALGENGVAAAFACSSFDVIAAHLW 300

Query: 301 KARTKAVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTI 360
           KARTKA+SVSKEKMVCLQFAVDARNKL+PPLPKGFSGNAFVLASVALTAKQLEEESYKTI
Sbjct: 301 KARTKALSVSKEKMVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLEEESYKTI 360

Query: 361 IEMIRNAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYA 420
           IEMIRNAKS+VDNDYINAY+ GLEGPQASLPPLKE TVVSDWTRMPFHKIEFLHG+A YA
Sbjct: 361 IEMIRNAKSQVDNDYINAYQHGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQATYA 420

Query: 421 SPLKPPILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           SPLKPPILEVAYFLQ+P DSQAIDV VPLL HT DAFSSYFL  MQ
Sbjct: 421 SPLKPPILEVAYFLQNPADSQAIDVHVPLLEHTFDAFSSYFLTRMQ 463

BLAST of Lcy12g017460 vs. NCBI nr
Match: XP_022941116.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 744.2 bits (1920), Expect = 6.7e-211
Identity = 385/461 (83.51%), Postives = 416/461 (90.24%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MATSH     VS+TKTLTVFPKTLN PKLLELSNLDRQCPLH+YLIFFY PS   S+SSF
Sbjct: 1   MATSH-----VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSF 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           DS+FTKLKIGLEETLS+W PAAGRLE +PA+GKLNLRC+NRGAILVQ+ TNV++S+LGDL
Sbjct: 61  DSVFTKLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNVEISKLGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFEKLVYKPVLNGNFSEFPLVVA QVTRFGCGGYSIGTGISHSLFDGPAAY+FLS
Sbjct: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVA-QVTRFGCGGYSIGTGISHSLFDGPAAYEFLS 180

Query: 181 AWAANSAISKDNKGQYGAQLHRPVHERGTLLMG---KRAAVASA-SVSGAVAIGHLYQLI 240
           AWAA SAI KD K  YG +LH PVHERG LLMG   KR+    A  VSGAVAI HLY+LI
Sbjct: 181 AWAAKSAIFKDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMPVSGAVAIDHLYRLI 240

Query: 241 MERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTK 300
           M+ +M  +EEMGKKEYV RTFR+SRGMIERLKK A+GENG FACSSFDVIAAHLWKARTK
Sbjct: 241 MQ-SMTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTK 300

Query: 301 AVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIR 360
           A+SVSKEKMVCLQFAV+ARNKL+PPLPKGFSGNAFVLASVALTA+QLE+ESYKTI++MIR
Sbjct: 301 AMSVSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIR 360

Query: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKP 420
           NAKSRVDNDYINAYKQGLEGPQASLPPLKE TVVSDWTRMPFHKIEFLHG+A YASPLKP
Sbjct: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKP 420

Query: 421 PILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           PIL+VAYFLQSP DSQAIDVRVPLL  TL+AFSSYFL  MQ
Sbjct: 421 PILDVAYFLQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of Lcy12g017460 vs. NCBI nr
Match: KAG6608286.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037638.1 Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 743.0 bits (1917), Expect = 1.5e-210
Identity = 384/461 (83.30%), Postives = 416/461 (90.24%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MATSH     VS+TKTLTVFPKTLN PKLLELSNLDRQCPLH+YLIFFY PS   S+SSF
Sbjct: 1   MATSH-----VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSF 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           DS+FTKLK+GLEETLS+W PAAGRLE +PA+GKLNLRC+NRGAILV++ TNV++SELGDL
Sbjct: 61  DSVFTKLKMGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVRSFTNVEISELGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFEKLVYKPVLNGNFSEFPLVVA QVTRFGCGGYSIGTGISHSLFDGPAAY+FLS
Sbjct: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVA-QVTRFGCGGYSIGTGISHSLFDGPAAYEFLS 180

Query: 181 AWAANSAISKDNKGQYGAQLHRPVHERGTLLMG---KRAAVASA-SVSGAVAIGHLYQLI 240
           AWAA SAI KD K  YG +LH PVHERG LLMG   KR+    A  VSGAVAI HLY+LI
Sbjct: 181 AWAAKSAIFKDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMPVSGAVAIDHLYRLI 240

Query: 241 MERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTK 300
           M+ +M  +EEMGKKEYV RTFR+SRGMIERLKK A+GENG FACSSFDVIAAHLWKARTK
Sbjct: 241 MQ-SMTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTK 300

Query: 301 AVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIR 360
           A+SVSKEKMVCLQFAV+ARNKL+PPLPKGFSGNAFVLASVALTA+QLE+ESYKTI++MIR
Sbjct: 301 AMSVSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIR 360

Query: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKP 420
           NAKSRVDNDYINAYKQGLEGPQASLPPLKE TVVSDWTRMPFHKIEFLHG+A YASPLKP
Sbjct: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKP 420

Query: 421 PILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           PIL+VAYFLQSP DSQAIDVRVPLL  TL+AFSSYFL  MQ
Sbjct: 421 PILDVAYFLQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of Lcy12g017460 vs. NCBI nr
Match: XP_022981142.1 (brassinosteroid-related acyltransferase 1 [Cucurbita maxima])

HSP 1 Score: 738.8 bits (1906), Expect = 2.8e-209
Identity = 382/463 (82.51%), Postives = 414/463 (89.42%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MATSH     VS+TKTLTVFPKTLN PKLLELSNLDRQCPLH+YLIFFY PS   S+SS 
Sbjct: 1   MATSH-----VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSL 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           DS+FT LKIGLEETLS+W PAAGRLE +PA+GKLNLRC+NRGAILVQ+ TN+++SELGDL
Sbjct: 61  DSIFTNLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFEKLVYKPVLNGNFSEFPLVVA QVTRFGCGGYSIGTGISHSLFDGPAAY+FLS
Sbjct: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVA-QVTRFGCGGYSIGTGISHSLFDGPAAYEFLS 180

Query: 181 AWAANSAISKDNKGQYGAQLHRPVHERGTLLM---GKRAAVASA---SVSGAVAIGHLYQ 240
           AWAA SAI KD K  YG +LH PVHERG LLM    KR++   A   SVSGAVAI HLY+
Sbjct: 181 AWAAKSAIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYR 240

Query: 241 LIMERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKAR 300
           LIM+ ++  +EEMGKKEYV RTFR+SRGMIERLKK A+GENG FACSSFDVIAAHLWKAR
Sbjct: 241 LIMQ-SVTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKAR 300

Query: 301 TKAVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEM 360
           TKA+SVS EKMVCLQFAV+ARNKL+PPLPKGFSGNAFVLASVALTA+QLE+ESYKTI+EM
Sbjct: 301 TKAMSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEM 360

Query: 361 IRNAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPL 420
           IRNAKSRVDNDYINAYKQGLEGPQASLPPLKE TVVSDWTRMPFHKIEFLHG+A YASPL
Sbjct: 361 IRNAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPL 420

Query: 421 KPPILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           KPPIL+VAYFLQSP DSQAIDVRVPLL  TL+AFSSYFL  MQ
Sbjct: 421 KPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 453

BLAST of Lcy12g017460 vs. NCBI nr
Match: XP_023524200.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 738.4 bits (1905), Expect = 3.7e-209
Identity = 382/461 (82.86%), Postives = 414/461 (89.80%), Query Frame = 0

Query: 1   MATSHESHSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSF 60
           MATSH     VS+TKTLTVFPKTLN PKLLELSNLDRQCPLH+YLIFFY PS   S+SS 
Sbjct: 1   MATSH-----VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSL 60

Query: 61  DSLFTKLKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDL 120
           DS+FT LKIGLEETLS+W PAAGRLE +PA+GKLNLRC+NRGAILVQ+ T+V++SELGDL
Sbjct: 61  DSVFTNLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTHVEISELGDL 120

Query: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLS 180
           SNYNEFFEKLVYKPVLNGNFSEFPLVVA QVTRFGCGGYSIGTGISHSLFDGPAAY+FLS
Sbjct: 121 SNYNEFFEKLVYKPVLNGNFSEFPLVVA-QVTRFGCGGYSIGTGISHSLFDGPAAYEFLS 180

Query: 181 AWAANSAISKDNKGQYGAQLHRPVHERGTLLMG---KRAAVASA-SVSGAVAIGHLYQLI 240
           AWAA SAI KD    YG +LH PVHERG LLMG   KR+    A  +SGAVAI HLY+LI
Sbjct: 181 AWAAKSAIFKDKNEPYGVELHTPVHERGRLLMGYDTKRSLEKRAMPLSGAVAIDHLYRLI 240

Query: 241 MERAMENEEEMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTK 300
           M+ +M  +EE+GKKEYV RTFR+SRGMIERLKK A+GENG FACSSFDVIAAHLWKARTK
Sbjct: 241 MQ-SMTGKEEIGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTK 300

Query: 301 AVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIR 360
           A+SVSKEKMVCLQFAV+ARNKL+PPLPKGFSGNAFVLASVALTA+QLE+ESYKTI+EMIR
Sbjct: 301 AMSVSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIR 360

Query: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKP 420
           NAKSRVDNDYINAYKQGLEGPQASLPPLKE TVVSDWTRMPFHKIEFLHG+A YASPLKP
Sbjct: 361 NAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKP 420

Query: 421 PILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFLASMQ 458
           PIL+VAYFLQSPTDSQAIDVRVPLL  TL+AFSSYFL  MQ
Sbjct: 421 PILDVAYFLQSPTDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of Lcy12g017460 vs. TAIR 10
Match: AT4G31910.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 416.4 bits (1069), Expect = 3.0e-116
Identity = 228/461 (49.46%), Postives = 317/461 (68.76%), Query Frame = 0

Query: 9   SQVSLTKTLTVFPKTLN--PPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTK 68
           +++ + + L V+P+  N    KL+ LSNLDRQCPL MY +FFY  +   +   FDS+F+ 
Sbjct: 7   TRIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNT---TTRDFDSVFSN 66

Query: 69  LKIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAI-TNVKVSELGDLSNYNE 128
           LK+GLEET+S+W PAAGRL L     KLN+RC++ GA++V+A+ T VK+SELGDL+ YNE
Sbjct: 67  LKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQYNE 126

Query: 129 FFEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAAN 188
           F+E LVYKP L+G+FS  PLVVA QVTRF CGGYSIG G SHSLFDG +AY+F+ AWA+N
Sbjct: 127 FYENLVYKPSLDGDFSVMPLVVA-QVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWASN 186

Query: 189 SAISKDNKGQYGAQ----LHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAM 248
           S I   +  +   +    + +PVH+R  LL+ + A       + A AI HLYQLI +  M
Sbjct: 187 SHIHNKSNSKITNKKEDVVIKPVHDRRNLLVNRDA----VRETNAAAICHLYQLIKQAMM 246

Query: 249 ENEE-----EMGKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTK 308
             +E     E+    +V +TF ++   IE +KKK+L     F CSSF+ +AAHLWKART+
Sbjct: 247 TYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSL---EGFMCSSFEFLAAHLWKARTR 306

Query: 309 AVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQ-LEEESYKTIIEMI 368
           A+ + ++ MVCLQFAVD R +   PLP+GFSGNA+VLASVA TA++ LEE + ++I+  I
Sbjct: 307 ALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELTLESIVNKI 366

Query: 369 RNAKSRVDNDYINAYKQGLEGP-QASLPPLKEHTVVSDWTRMPFHKIEFLHG--EAAYAS 428
           R AK  +D  YIN+Y + L G    +LPPLKE T++SDWT+MPFH + F +G   A Y +
Sbjct: 367 REAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNGGEPADYMA 426

Query: 429 PLKPPILEVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFL 454
           PL PP+ +VAYF+++P D++ + VR+ L    ++ FS++FL
Sbjct: 427 PLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHFL 456

BLAST of Lcy12g017460 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 174.5 bits (441), Expect = 2.0e-43
Identity = 132/454 (29.07%), Postives = 220/454 (48.46%), Query Frame = 0

Query: 8   HSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTKL 67
           H +V   +   V P++     L  LSNLD+   + +  I+ +      +  +       +
Sbjct: 30  HLEVHQKEPALVKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQ----VI 89

Query: 68  KIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDLSNYN-EF 127
           K  L + L  + P AGRL +SP +GKL + C   G + V+A  N K+ E+GD++  + E 
Sbjct: 90  KKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPET 149

Query: 128 FEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANS 187
             KLVY  V   N  E P V A QVT+F CGG+ +G  ++H +FDG  A +F+++W   +
Sbjct: 150 LGKLVYDVVDAKNILEIPPVTA-QVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVA 209

Query: 188 AISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEE 247
                     G  L  P     T+L  +              I +L+Q   E  +E++  
Sbjct: 210 R---------GLPLTTPPFSDRTILNARNPP----------KIENLHQEFEE--IEDKSN 269

Query: 248 MG----KKEYVCRTFRVSRGMIERLKKKALGENGAF---ACSSFDVIAAHLWKARTKAVS 307
           +     K+  + R+F      I++LK +A   + +    +C+SF+ ++A +W+ARTK++ 
Sbjct: 270 INSLYTKEPTLYRSFCFDPEKIKKLKLQATENSESLLGNSCTSFEALSAFVWRARTKSLK 329

Query: 308 VSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAK 367
           +  ++   L FAVD R K  P LPKG+ GN  VL +    A +L E+     + ++R A 
Sbjct: 330 MLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAI 389

Query: 368 SRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKPPIL 427
             V + Y+ +     E  +A  P L    +++ W+R+ FH  +F  GE   + P+  P  
Sbjct: 390 KMVTDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEK 449

Query: 428 EVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFL 454
           EV  FL      ++I+V + L +  +D F   FL
Sbjct: 450 EVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFL 455

BLAST of Lcy12g017460 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 174.5 bits (441), Expect = 2.0e-43
Identity = 132/454 (29.07%), Postives = 220/454 (48.46%), Query Frame = 0

Query: 8   HSQVSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTKL 67
           H +V   +   V P++     L  LSNLD+   + +  I+ +      +  +       +
Sbjct: 14  HLEVHQKEPALVKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQ----VI 73

Query: 68  KIGLEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNVKVSELGDLSNYN-EF 127
           K  L + L  + P AGRL +SP +GKL + C   G + V+A  N K+ E+GD++  + E 
Sbjct: 74  KKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPET 133

Query: 128 FEKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANS 187
             KLVY  V   N  E P V A QVT+F CGG+ +G  ++H +FDG  A +F+++W   +
Sbjct: 134 LGKLVYDVVDAKNILEIPPVTA-QVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVA 193

Query: 188 AISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEE 247
                     G  L  P     T+L  +              I +L+Q   E  +E++  
Sbjct: 194 R---------GLPLTTPPFSDRTILNARNPP----------KIENLHQEFEE--IEDKSN 253

Query: 248 MG----KKEYVCRTFRVSRGMIERLKKKALGENGAF---ACSSFDVIAAHLWKARTKAVS 307
           +     K+  + R+F      I++LK +A   + +    +C+SF+ ++A +W+ARTK++ 
Sbjct: 254 INSLYTKEPTLYRSFCFDPEKIKKLKLQATENSESLLGNSCTSFEALSAFVWRARTKSLK 313

Query: 308 VSKEKMVCLQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAK 367
           +  ++   L FAVD R K  P LPKG+ GN  VL +    A +L E+     + ++R A 
Sbjct: 314 MLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAI 373

Query: 368 SRVDNDYINAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKPPIL 427
             V + Y+ +     E  +A  P L    +++ W+R+ FH  +F  GE   + P+  P  
Sbjct: 374 KMVTDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEK 433

Query: 428 EVAYFLQSPTDSQAIDVRVPLLSHTLDAFSSYFL 454
           EV  FL      ++I+V + L +  +D F   FL
Sbjct: 434 EVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFL 439

BLAST of Lcy12g017460 vs. TAIR 10
Match: AT3G48720.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 170.2 bits (430), Expect = 3.7e-42
Identity = 115/419 (27.45%), Postives = 204/419 (48.69%), Query Frame = 0

Query: 32  LSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTKLKIGLEETLSIWPPAAGRLELSPAD 91
           LSNLD+   + +  +++Y   S  +  S++     +K  L E L  + P AGRL +SP +
Sbjct: 30  LSNLDQNIAIIVKTLYYYKSESRTNQESYN----VIKKSLSEVLVHYYPVAGRLTISP-E 89

Query: 92  GKLNLRCDNRGAILVQAITNVKVSELGDLSNYN--EFFEKLVYKPVLNGNFSEFPLVVAQ 151
           GK+ + C   G ++V+A  N  +  + +  + N  E  EKLVY      N  E P VV  
Sbjct: 90  GKIAVNCTGEGVVVVEAEANCGIDTIKEAISENRMETLEKLVYDVPGARNILEIPPVVV- 149

Query: 152 QVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANSAISKDNKGQYGAQLHRPVHERGT 211
           QVT F CGG+ +G G+SH++FDG AA +FL++W   +          G  L  P     T
Sbjct: 150 QVTNFKCGGFVLGLGMSHNMFDGVAAAEFLNSWCEMAK---------GLPLSVPPFLDRT 209

Query: 212 LLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEEMGKKEYVCRTFRVSRGMIERLKK 271
           +L  +            +   H     +E   +  +   +++ + ++F      +E+LK 
Sbjct: 210 ILRSRNPP--------KIEFPHNEFDEIEDISDTGKIYDEEKLIYKSFLFEPEKLEKLKI 269

Query: 272 KALGENGAFACSSFDVIAAHLWKARTKAVSVSKEKMVCLQFAVDARNKLIPPLPKGFSGN 331
            A+ EN     S+F  +   LWK+R +A+    ++ V L FA D R++ IP LP+G+ GN
Sbjct: 270 MAIEENNNNKVSTFQALTGFLWKSRCEALRFKPDQRVKLLFAADGRSRFIPRLPQGYCGN 329

Query: 332 AFVLASVALTAKQLEEESYKTIIEMIRNAKSRVDNDYINAYKQGLEGPQASLPPLKEHTV 391
             VL  +  ++ +L        + +++     V + ++ +     E    + P +    +
Sbjct: 330 GIVLTGLVTSSGELVGNPLSHSVGLVKRLVELVTDGFMRSAMDYFE-VNRTRPSMNATLL 389

Query: 392 VSDWTRMPFHKIEFLHGEAAYASPLKPPILEVAYFLQSPTDSQAIDVRVPLLSHTLDAF 449
           ++ W+++  HK++F  GE  ++ P+  P  EV  FL S  D ++I+V + L +  ++ F
Sbjct: 390 ITSWSKLTLHKLDFGWGEPVFSGPVGLPGREVILFLPSGDDMKSINVFLGLPTSAMEVF 424

BLAST of Lcy12g017460 vs. TAIR 10
Match: AT5G63560.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 162.2 bits (409), Expect = 1.0e-39
Identity = 122/441 (27.66%), Postives = 209/441 (47.39%), Query Frame = 0

Query: 11  VSLTKTLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVPSNSSFDSLFTKLKIG 70
           V+  + + V P +  P  L  LSNLD+   + +   +++  +S  +  S++     +K  
Sbjct: 9   VTRKEPVLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNSRSNEESYE----VIKKS 68

Query: 71  LEETLSIWPPAAGRLELSPADGKLNLRCDNRGAILVQAITNV---KVSELGDLSNYNEFF 130
           L E L  + PAAGRL +SP +GK+ + C   G ++V+A  N    K+ +     +  E  
Sbjct: 69  LSEVLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETL 128

Query: 131 EKLVYKPVLNGNFSEFPLVVAQQVTRFGCGGYSIGTGISHSLFDGPAAYDFLSAWAANSA 190
           EKLVY      N  E P VV  QVT F CGG+ +G G++H++FDG AA +FL++WA  + 
Sbjct: 129 EKLVYDVPGARNILEIPPVVV-QVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETAR 188

Query: 191 ISKDNKGQYGAQLHRPVHERGTLLMGKRAAVASASVSGAVAIGHLYQLIMERAMENEEEM 250
                    G  L  P     TLL  +            +   H     +E      +  
Sbjct: 189 ---------GLPLSVPPFLDRTLLRPR--------TPPKIEFPHNEFEDLEDISGTGKLY 248

Query: 251 GKKEYVCRTFRVSRGMIERLKKKALGENGAFACSSFDVIAAHLWKARTKAVSVSKEKMVC 310
             ++ V ++F      +ERLK  A   +     ++F  +   LW+AR +A+ +  ++ + 
Sbjct: 249 SDEKLVYKSFLFGPEKLERLKIMAETRS-----TTFQTLTGFLWRARCQALGLKPDQRIK 308

Query: 311 LQFAVDARNKLIPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMIRNAKSRVDNDYI 370
           L FA D R++ +P LPKG+SGN  V      TA ++        + +++ A   V++ ++
Sbjct: 309 LLFAADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDGFM 368

Query: 371 NAYKQGLEGPQASLPPLKEHTVVSDWTRMPFHKIEFLHGEAAYASPLKPPILEVAYFLQS 430
            +     E  +A  P L    +++ W ++ FH  +F  GE   + P+  P  EV  FL  
Sbjct: 369 RSAIDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFLPC 420

Query: 431 PTDSQAIDVRVPLLSHTLDAF 449
            +D+++I+V + L    +  F
Sbjct: 429 GSDTKSINVLLGLPGSAMKVF 420