Lcy12g015350 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g015350 vs. ExPASy Swiss-Prot
Match: Q9SB51 (Ubiquitin carboxyl-terminal hydrolase 16 OS=Arabidopsis thaliana OX=3702 GN=UBP16 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 8.6e-177
Identity = 432/1050 (41.14%), Postives = 595/1050 (56.67%), Query Frame = 0

Query: 1   MLVSGSIGLQ--VLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEAS 60
           ML+   +G+   VL+V +V P+I L +R KWR++  R+EEI RLL+ +SEEA RAELEAS
Sbjct: 1   MLLVLDLGISSLVLVVSLVLPLIGLFVRHKWRVAAQRREEIRRLLIHASEEAARAELEAS 60

Query: 61  AGYISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSI--- 120
             + S + +   H C VCY   TTRC+RCKAVRYCSGKCQIIHWRQGHK+ CHP SI   
Sbjct: 61  VEFSSVAVSNVFH-CPVCYCLATTRCSRCKAVRYCSGKCQIIHWRQGHKDECHPASIVYD 120

Query: 121 ----------------------------------------SNRTVASRTDFEQKVTEQ-- 180
                                                   SNR   S  D    + +   
Sbjct: 121 SEDESDSDLRLGEENGQNTPEETLLVGPEPVTIPIGESLLSNR-ARSPEDGNGDIADNKD 180

Query: 181 ---DHYES-STSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDGYMSADATPD 240
              D  E+ S +++S +SFSGFS+S   ++S D+ S  +S S  E ++ +  + A  + +
Sbjct: 181 DLIDKEEAVSVAETSGSSFSGFSSSP-RNDSGDEISRCESFSSSESERSESLLDAHVSVE 240

Query: 241 SLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSRSSINSSSFV 300
             +          +K LSP F +LV+SVD+  +  KL+  KP    G++   S +  S V
Sbjct: 241 PEDTCFSTIEDAPSKLLSPKFVHLVESVDNLANLPKLSVHKPEDDAGQNQSQSRSLHSLV 300

Query: 301 INGNHKDSDATV-SSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPSSFSFNSSN 360
            + +   +D ++ SSDFW   +      SDS        +     R  +S   FSF S  
Sbjct: 301 TDRHPVSADPSLKSSDFWGTALGSAERVSDSC-------VKSKSGRPGNSSLHFSFGSG- 360

Query: 361 VLQARSSEEKNASNITPPGASEDKKDKKPV----NGENLSKNYSIPSNVCNSAPSNYQGH 420
                SS + +A+ ++   +S  K+  +      +G NL +                   
Sbjct: 361 -----SSRDTSAAKVSEQRSSILKEAPRGTGYISDGVNLRE------------------- 420

Query: 421 KKTGNFKPASNSHVRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGPIDTIS 480
           +    F  A  +   S ST ++ P + S      S+V++     +   N  +  P+  + 
Sbjct: 421 RNAKRFDEAEIALPISSSTDALSPLDSSN----LSHVTLPKSKSASSENGSMLAPL-KVG 480

Query: 481 KVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACTLNSKN 540
           +VQ      S  K  ++    S  G  SV        RV   Q  +G   Q   +L+ ++
Sbjct: 481 EVQLLASKASNTKKCADLMKHSPLGAKSV--------RVLDHQKQNGAVVQHINSLHGRS 540

Query: 541 GLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLELQPAG 600
           GL+ S+LKVVDQ+   K        +  E+ GR  +KGLF Y+ F KLY + K+E QP G
Sbjct: 541 GLKASVLKVVDQWTRPK--------SENEMAGRHGHKGLFPYEVFAKLYTY-KIEFQPCG 600

Query: 601 LINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLIAPLYI 660
           LIN GNSC+ANVV QCL FTPPLT YFLQ  HS++C                        
Sbjct: 601 LINVGNSCFANVVFQCLMFTPPLTTYFLQQFHSRAC------------------------ 660

Query: 661 YIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEEDAHEFL 720
                  TKK  CF C FE L++K KE +SPLSP G++SQL +IG  LGNGKEEDAHEFL
Sbjct: 661 -------TKKEQCFTCGFEKLVVKAKEEKSPLSPNGLLSQLQNIGIFLGNGKEEDAHEFL 720

Query: 721 RYAIDTMQSLCCMESGVSSSGSWE-EETTLVGLTFGGYILSKIKCTRCQGRSERQERIMD 780
           R+ +DTMQS+C   S    + S + E+TTL+GLTFGGY+ SKIKC +CQ +SE +E++MD
Sbjct: 721 RFVVDTMQSVCIKASEYDMTKSSKLEDTTLIGLTFGGYLRSKIKCMKCQVKSELREKMMD 780

Query: 781 LTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILTVVLK 840
           LTVEI+GDI  L++ALR+FT TEILDG+NKY C  CK+Y RAKKKLKI E PN+LT+ LK
Sbjct: 781 LTVEIDGDISTLDDALRRFTRTEILDGENKYRCGSCKSYERAKKKLKITEPPNVLTIALK 840

Query: 841 RFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHYVCYV 900
           RFQ+G FGKLNK I+FPE LD+AP+V G S+KS  Y+LYGV+VHLDVMN++FSGHYVCY+
Sbjct: 841 RFQAGKFGKLNKLIRFPETLDLAPYVSGGSEKSHDYKLYGVIVHLDVMNAAFSGHYVCYI 900

Query: 901 KNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRL-LRHGVGAPDSKTRTTH 960
           +N +NKW+K DDSTV   D+E +LTKGAYML Y+RC P  PRL +     A + K+R   
Sbjct: 901 RN-QNKWYKADDSTVVTSDVERILTKGAYMLFYARCTPTPPRLAVCTKTEASNKKSRVPL 956

Query: 961 SRTDERNTTAKTTTTSTHQSYTKDTP--GEPGSIGSIHFGHPQILRIFEEDSSSDNSSLI 991
            + +E++T +++ +TS+ +  + +TP  G  G+I S +    ++ +I EEDS+SD+SSL 
Sbjct: 961 PKANEKSTISRSVSTSSPE-LSSNTPGGGRSGNIQSFYSSFQRLQKILEEDSASDSSSLF 956

BLAST of Lcy12g015350 vs. ExPASy Swiss-Prot
Match: Q9FKP5 (Ubiquitin carboxyl-terminal hydrolase 17 OS=Arabidopsis thaliana OX=3702 GN=UBP17 PE=2 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 1.9e-131
Identity = 363/987 (36.78%), Postives = 490/987 (49.65%), Query Frame = 0

Query: 20  VICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAE--LEASAGYISSSFTPPGHQCAVCY 79
           ++ L+IRR+WR +  R+EE+ RL+ L++EE++ AE    A+  Y   S +   ++CAVC 
Sbjct: 3   LVFLLIRRQWRSASVRREEVIRLIALATEESYLAEEVRPATVDYGGDSVSDV-YRCAVCL 62

Query: 80  SPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTVASRTDFEQKVTEQDHYE 139
            PTTTRC++CK+VRYCS KCQI+HWR+GHKE C  P           D E++   Q  Y+
Sbjct: 63  YPTTTRCSQCKSVRYCSSKCQILHWRRGHKEECRSPDY---------DEEKEEYVQSDYD 122

Query: 140 SSTS--DSSTTSFSGFSTSNIGSESSDDTSTS-DSSSQVEPDKFDGYMSADATPDSLEIT 199
           +  S  D  +   +  S+SN+  + + D STS  S  +V+P             ++++ T
Sbjct: 123 AKESNVDFPSRGTAYESSSNVSVDVACDMSTSRPSIHKVQP-----------RSEAVDFT 182

Query: 200 RGIDVAD---QTKPLS-PMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSRSSINSSSFVI 259
             +++ D   +T+PLS     N  D V+S  + S                          
Sbjct: 183 TSLNIKDNLYETRPLSRKKSRNRTDKVESASNYS-------------------------- 242

Query: 260 NGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPSSFSFNSSNVL 319
                                 KG T     D  L KL   +SR+    ++ S  S   L
Sbjct: 243 ----------------------KGKT-----DAKLRKLGNQNSRRSGDSANMSI-SDQFL 302

Query: 320 QARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSNYQGHKKTGNF 379
                EE NA                        K+  I S                   
Sbjct: 303 SVGFEEEMNA-----------------------LKHERITS------------------- 362

Query: 380 KPASNSHVRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGPIDTISKVQGKD 439
           +P+S S   S S++ + P                                          
Sbjct: 363 EPSSASAAMSSSSTLLLP------------------------------------------ 422

Query: 440 GNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACTLNSKNGLRTSM 499
                 K NS+  +  +                                  S +GL+TS+
Sbjct: 423 -----SKANSKPKVSQA----------------------------------SSSGLKTSV 482

Query: 500 LKVVDQFRGSKLLKNNQ-ACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLELQPAGLINCG 559
            KVV  FR  +  K +Q + ++ E+         FSY+ FVKLY  +++ELQP GL+N G
Sbjct: 483 QKVVQHFRPPQSSKKSQPSSSIDEMS--------FSYELFVKLY-CDRVELQPFGLVNLG 542

Query: 560 NSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLIAPLYIYIFSI 619
           NSCYAN VLQCLAFT PL +Y ++GLHSK+C                             
Sbjct: 543 NSCYANAVLQCLAFTRPLISYLIRGLHSKTC----------------------------- 602

Query: 620 SGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEEDAHEFLRYAID 679
              KK WCF CEFE LILK + G SPLSP+ I+S+L  IG+ LG GKEEDAHEFLR A+D
Sbjct: 603 --RKKSWCFVCEFEHLILKARGGESPLSPIKILSKLQKIGKHLGPGKEEDAHEFLRCAVD 662

Query: 680 TMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQERIMDLTVEIE 739
           TMQS+   E+   ++G + EETTLVGLTFGGY+ SKIKC  C  +SER E +MDLTVEI+
Sbjct: 663 TMQSVFLKEA--PAAGPFAEETTLVGLTFGGYLHSKIKCMACLHKSERPELMMDLTVEID 719

Query: 740 GDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILTVVLKRFQSGN 799
           GDIG LEEAL +FT+ E+LDG+N+Y C RCK+Y +AKKKL ILE PNILTVVLKRFQS N
Sbjct: 723 GDIGSLEEALAQFTAYEVLDGENRYFCGRCKSYQKAKKKLMILEGPNILTVVLKRFQSDN 719

Query: 800 FGKLNKPIKFPEILDMAPFVRGTS-DKSPVYRLYGVVVHLDVMNSSFSGHYVCYVKNAKN 859
           FGKL+KPI FPE+LD++P++   +    PVY LY VVVHLD M++ FSGHYVCY+K    
Sbjct: 783 FGKLSKPIHFPELLDISPYMSDPNHGDHPVYSLYAVVVHLDAMSTLFSGHYVCYIKTLDG 719

Query: 860 KWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTRTTHSRTDER 919
            WFKIDDS V PV +E+VL +GAYMLLY+R  PR           P SK     S+   R
Sbjct: 843 DWFKIDDSNVFPVQLETVLLEGAYMLLYARDSPR-----------PVSKNGGRKSK-QRR 719

Query: 920 NTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSLISSTSDEGS 979
           N  A      + +   K   G+  S+       P++      D SS + S + S+SD  +
Sbjct: 903 NLAA----IPSRKGNKKQRDGDNNSL------LPRV------DWSSGSLSSMFSSSD--T 719

Query: 980 YSTESTRDSTSADELSDYIFGDPGRVW 996
            S+ ST+DS+  + LSDY+FG    VW
Sbjct: 963 TSSCSTKDSSGIENLSDYLFGGVEPVW 719

BLAST of Lcy12g015350 vs. ExPASy Swiss-Prot
Match: Q9FPS9 (Ubiquitin carboxyl-terminal hydrolase 15 OS=Arabidopsis thaliana OX=3702 GN=UBP15 PE=1 SV=2)

HSP 1 Score: 366.7 bits (940), Expect = 8.5e-100
Identity = 319/1033 (30.88%), Postives = 441/1033 (42.69%), Query Frame = 0

Query: 11  VLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAE---------------- 70
           + LV VV PV+  ++  KW     ++   N L  +++EEA RAE                
Sbjct: 12  LFLVLVVLPVVAYILLGKWSNISEKRVRANLLAQMAAEEALRAETVVNADRGVRFESVAT 71

Query: 71  -----------LEASAGYISSSFTPPG-----------------------------HQCA 130
                      + A  G + + F                                 H CA
Sbjct: 72  ENRAQRTRTKTVSAGGGAVRAEFDAGARETVAEQRSDSVTATCGVTVVAPVNNNELHVCA 131

Query: 131 VCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTVASRTDFEQKVTEQD 190
            C+ P  TRC+RCK+VRYCSGKCQIIHWR  HK+ C P   S  + + R  FE+     D
Sbjct: 132 RCFGPAKTRCSRCKSVRYCSGKCQIIHWRVAHKDECVPVE-SCSSSSERVSFEKDSVLYD 191

Query: 191 HYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDGYMSADATPDSLEIT 250
           H   ST  S+ T+ +    +   S+SS D +                            +
Sbjct: 192 HGMDSTMYSNNTTQAAKGKT---SKSSVDFA----------------------------S 251

Query: 251 RGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSRSSINSSSFVINGNH 310
            GI   D T  ++      V        SSK N+     S  +DS +  +SS        
Sbjct: 252 LGISQNDITPQINTQGRKSVGK----QHSSKANR----ESCRRDSATVFDSS-------- 311

Query: 311 KDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPSSFSFNSSNVLQARS 370
            D  A+   D     I HK S  +S   +T P+      R     S+   N  + +    
Sbjct: 312 -DEAASAGGDNKTSHIKHK-SRGNSYAAETNPR------RHSVDSSAVQMNGQSFVSGMQ 371

Query: 371 SEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSNYQGHKKTGNFKPAS 430
              K+ +N+              V       N   PSN   +A     G  K+G      
Sbjct: 372 ESHKHENNL-------------GVRSSFGCPNTQYPSNGTRTATLPRTGINKSGEQSCTE 431

Query: 431 NSHVRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGPIDTISK-VQGKDGNF 490
            S                                         G +  +SK V+ KD   
Sbjct: 432 TSK---------------------------------------KGQVAAVSKTVRSKDTGI 491

Query: 491 SQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACTLNSKNGLRTSMLKV 550
           S+                            + + +T GI   +        GLR S  K 
Sbjct: 492 SEES--------------------------NGISSTMGIMKMM--------GLRNS-TKH 551

Query: 551 VDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLELQPAGLINCGNSCY 610
            D+++                    N K LF Y+ F+K +     +L P GL+NCGNSCY
Sbjct: 552 DDRYK--------------------NLKMLFPYEEFLKFFQCEVFDLSPRGLVNCGNSCY 611

Query: 611 ANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLIAPLYIYIFSISGTK 670
           AN VLQ L  T PL AY L+  HS+SC                               + 
Sbjct: 612 ANAVLQSLTCTKPLVAYLLRRSHSRSC-------------------------------SG 671

Query: 671 KGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEEDAHEFLRYAIDTMQS 730
           K WC  CE E  ++ ++E   PLS   I+S + SI  Q+G+G +EDAHEFLR  + +MQS
Sbjct: 672 KDWCLMCELEQHVMMLRESGGPLSASRILSHMRSINCQIGDGSQEDAHEFLRLLVASMQS 731

Query: 731 LCCME-SGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQERIMDLTVEIEGDI 790
           +C     G +      +ETTLV   FGG + SK+KC RC   SER E IMDLT+EI G +
Sbjct: 732 ICLERLGGETKVDPRLQETTLVQHMFGGRLRSKVKCLRCDHESERYENIMDLTLEIYGWV 791

Query: 791 GRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILTVVLKRFQSGNFGK 850
             L++AL +FT  E LDG+N Y CSRC  YVRA+K+L I EAPNILT+VLKRFQ G +GK
Sbjct: 792 ESLQDALTQFTRPEDLDGENMYRCSRCAGYVRARKELSIHEAPNILTIVLKRFQEGRYGK 821

Query: 851 LNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHYVCYVKNAKNKWFK 910
           +NK I FPE+LDM PF+  T D  P+Y LY V+VHLD +N+SFSGHY+ YVK+ +  W++
Sbjct: 852 INKCISFPEMLDMIPFMTRTGDVPPLYMLYAVIVHLDTLNASFSGHYISYVKDLRGNWYR 821

Query: 911 IDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTRTTHSRTDERNTTA 970
           IDDS + PV +  V+++GAYML Y R  PR P+   H   AP   ++  +   ++R    
Sbjct: 912 IDDSEIHPVPMTQVMSEGAYMLFYMRSYPR-PQRGEHNGKAPVHHSQPRNEMKEQRKPVN 821

Query: 971 KTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSLISSTSDEGSYSTE 986
           +    + H++                            +SSS   SL +S SDE S++TE
Sbjct: 972 RFKPRADHKN---------------------------TESSSSEWSLFTS-SDEASFTTE 821

BLAST of Lcy12g015350 vs. ExPASy Swiss-Prot
Match: Q9SJA1 (Ubiquitin carboxyl-terminal hydrolase 19 OS=Arabidopsis thaliana OX=3702 GN=UBP19 PE=2 SV=2)

HSP 1 Score: 365.2 bits (936), Expect = 2.5e-99
Identity = 183/386 (47.41%), Postives = 243/386 (62.95%), Query Frame = 0

Query: 523 LFSYDHFVKLYNWNKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTF 582
           LF Y+ FV+ YNW++  + P GL NCGNSC+ANVVLQCL++T PL AY L+  H + C  
Sbjct: 154 LFPYESFVRYYNWDRPIMAPCGLTNCGNSCFANVVLQCLSWTRPLVAYLLERGHKREC-- 213

Query: 583 QITGSFSAVSNFQNLLIAPLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGII 642
                                         +  WCF CEFE+ + +    R P SP+ II
Sbjct: 214 -----------------------------RRNDWCFLCEFENHLDRANYSRFPFSPMNII 273

Query: 643 SQLPSIGRQLGNGKEEDAHEFLRYAIDTMQSLCCME-SGVSSSGSWEEETTLVGLTFGGY 702
           S+LP+IG  LG G++EDAHE +R+AID MQS+C  E  G        +ETTL+   FGG 
Sbjct: 274 SRLPNIGGNLGYGRQEDAHELMRFAIDMMQSVCLDEFGGEKVVPPRAQETTLIQYIFGGL 333

Query: 703 ILSKIKCTRCQGRSERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKT 762
           + S+++CT C   S++ E +MDLTVEI GD   LEE L +FT+ E L GDN Y C RC  
Sbjct: 334 LQSQVQCTACSNVSDQYENMMDLTVEIHGDAVSLEECLDQFTAKEWLQGDNLYKCDRCDD 393

Query: 763 YVRAKKKLKILEAPNILTVVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRL 822
           YV+A K+L I  APNILT+ LKRFQ G FGKLNK I FPE  D+ P++ G  + S VY+L
Sbjct: 394 YVKACKRLSIRCAPNILTIALKRFQGGRFGKLNKRISFPETFDLGPYMSGGGEGSDVYKL 453

Query: 823 YGVVVHLDVMNSSFSGHYVCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLP 882
           Y V+VHLD++N+SF GHY+CYVK+ +  W++IDDS V  V++E VL++ AYMLLYSR  P
Sbjct: 454 YAVIVHLDMLNASFFGHYICYVKDFRGNWYRIDDSEVEKVELEDVLSQRAYMLLYSRVQP 507

Query: 883 RAPRLLRHGVGAPDSKTRTTHSRTDE 908
           R P  LR      + KT T ++ +++
Sbjct: 514 R-PSNLRSEESQDEKKTDTLNTESNQ 507

HSP 2 Score: 55.8 bits (133), Expect = 3.2e-06
Identity = 23/56 (41.07%), Postives = 33/56 (58.93%), Query Frame = 0

Query: 72  QCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTVASRTDFE 128
           +C+VC   TT +C+RCK+VRYCS  CQ   W+ GHK  C     ++ +   R D +
Sbjct: 63  ECSVCGKATTKKCSRCKSVRYCSAACQTSDWKSGHKLKCKGFRSTDSSPVRRDDID 118

BLAST of Lcy12g015350 vs. ExPASy Swiss-Prot
Match: Q67XW5 (Ubiquitin carboxyl-terminal hydrolase 18 OS=Arabidopsis thaliana OX=3702 GN=UBP18 PE=2 SV=2)

HSP 1 Score: 362.8 bits (930), Expect = 1.2e-98
Identity = 185/423 (43.74%), Postives = 253/423 (59.81%), Query Frame = 0

Query: 479 TLNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKL 538
           T ++KNGLR       D    S  L + +    + +  R   + LF Y+ FV+ +NW+  
Sbjct: 107 TPSAKNGLRFRASPFGDSSASSIALISERGQNKSSLKPR---EVLFPYEEFVEYFNWDNP 166

Query: 539 ELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLL 598
           EL P GL+NCGNSC+ANV+LQCL++T PL AY L+  H + C                  
Sbjct: 167 ELAPCGLMNCGNSCFANVILQCLSWTRPLVAYLLEKGHKREC------------------ 226

Query: 599 IAPLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEE 658
                         +  WCF CEF++ + +  + R P SP+ IIS+L +IG  LG G++E
Sbjct: 227 -------------MRNDWCFLCEFQTHVERASQSRFPFSPMNIISRLTNIGGTLGYGRQE 286

Query: 659 DAHEFLRYAIDTMQSLCCME-SGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSER 718
           DAHEF+RYAID MQS+C  E  G        +ETTL+   FGG + S+++CT C   S++
Sbjct: 287 DAHEFMRYAIDMMQSVCLDEFGGEKIVPPRSQETTLIQYIFGGLLQSQVQCTVCNHVSDQ 346

Query: 719 QERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNI 778
            E +MDL VE+ GD G LEE L +FT+ E L GDN Y C RC  YV+A K+L I  APNI
Sbjct: 347 YENMMDLIVEMHGDAGSLEECLDQFTAEEWLHGDNMYKCDRCSDYVKACKRLTIRRAPNI 406

Query: 779 LTVVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSG 838
           LT+ LKR+Q G +GKLNK I FPE LD+ P++    D S VY+LY V+VHLD++N+SF G
Sbjct: 407 LTIALKRYQGGRYGKLNKRISFPETLDLNPYMSEGGDGSDVYKLYAVIVHLDMLNASFFG 466

Query: 839 HYVCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSK 898
           HY+CY+K+    W++IDDS +  V++E VL++ AYMLLYSR   R+           + K
Sbjct: 467 HYICYIKDFCGNWYRIDDSEIESVELEDVLSQRAYMLLYSRIQARSSSSCLRSEVKDEKK 495

Query: 899 TRT 901
           T T
Sbjct: 527 TDT 495

HSP 2 Score: 50.4 bits (119), Expect = 1.3e-04
Identity = 30/100 (30.00%), Postives = 51/100 (51.00%), Query Frame = 0

Query: 57  ASAGYISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSIS 116
           A+ G+           C+VC + +T +C+RCK+VRYCS +CQ   W  GH+ NC    I+
Sbjct: 45  AAGGFDRDFMAVDAEDCSVCGNFSTKKCSRCKSVRYCSAECQRSDWSSGHQRNCRDYGIT 104

Query: 117 NRTVASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNI 157
             T +++     + +     +SS S  +  S  G + S++
Sbjct: 105 TLTPSAKNGLRFRAS--PFGDSSASSIALISERGQNKSSL 142

BLAST of Lcy12g015350 vs. ExPASy TrEMBL
Match: A0A0A0LE16 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G890110 PE=3 SV=1)

HSP 1 Score: 1682.2 bits (4355), Expect = 0.0e+00
Identity = 876/1014 (86.39%), Postives = 916/1014 (90.34%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSG+IGLQVL VFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAE+EASA 
Sbjct: 1    MLVSGNIGLQVLFVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAEIEASAR 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y SSSFTP GHQCAVCYSPTTTRC+RCKAVRYCSGKCQIIHWRQGHKENCHPP   N  V
Sbjct: 61   YTSSSFTPLGHQCAVCYSPTTTRCSRCKAVRYCSGKCQIIHWRQGHKENCHPP---NHAV 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            AS TDFE KVTEQDHYESSTSDSS  SFSGFS+S + SESSDDTSTSDSSSQ+EP+K DG
Sbjct: 121  ASSTDFETKVTEQDHYESSTSDSSAASFSGFSSSILSSESSDDTSTSDSSSQIEPEKSDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            YMSADA PDSLEITRGID+ADQ K L+PMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR
Sbjct: 181  YMSADAMPDSLEITRGIDIADQPKSLTPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
            SS+NSSSFVI+GNHK+S ATVSSDFWART+ HKGST+DSL +  LPK SGGH RKLSSP 
Sbjct: 241  SSVNSSSFVIDGNHKESVATVSSDFWARTLDHKGSTTDSLSNSALPKFSGGHGRKLSSPP 300

Query: 301  SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
            SFSF+SSN+LQA SSE  NASNIT PG SEDKK KKPV+GEN SKN+SIPSNVCNSA SN
Sbjct: 301  SFSFSSSNILQACSSEGMNASNITLPGTSEDKKGKKPVDGENFSKNHSIPSNVCNSAASN 360

Query: 361  YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
             QGHKKTGNFKPAS SHV RSRSTS+VRPPNGSGGKTLDSNVS SPPL+ ERS KVV   
Sbjct: 361  NQGHKKTGNFKPASTSHVLRSRSTSAVRPPNGSGGKTLDSNVSTSPPLRCERSAKVV--- 420

Query: 421  IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
            +D+ISKVQ KDGNFSQRK++SEC +PSSAGGTSVSLSRIQSGRVD +QATSGIPSQVACT
Sbjct: 421  VDSISKVQSKDGNFSQRKIDSECCLPSSAGGTSVSLSRIQSGRVDTMQATSGIPSQVACT 480

Query: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
            LNSKNGLRTSMLKVVDQFRGSKLLKN+ ACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE
Sbjct: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNSHACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540

Query: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
            LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601  APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                         KKGWCFNCEFESLILKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601  ------------VKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661  AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
            AHEFLRY IDTMQS+CC E GVSSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661  AHEFLRYVIDTMQSICCKEYGVSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721  RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
            RIMDLTVEIEGDIGRL+EALRKFTS EILDGDNKYLCSRCK+YVRAKKKLKILEAPNILT
Sbjct: 721  RIMDLTVEIEGDIGRLDEALRKFTSKEILDGDNKYLCSRCKSYVRAKKKLKILEAPNILT 780

Query: 781  VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
            VVLKRFQSGNFGKLNKPIKFPEILD+APF+RGTSDKSP+YRLYGVVVHLDVMNSSFSGHY
Sbjct: 781  VVLKRFQSGNFGKLNKPIKFPEILDLAPFIRGTSDKSPIYRLYGVVVHLDVMNSSFSGHY 840

Query: 841  VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
            VCYVKNAKNKWFKIDDSTVTPVDIE+VLTKGAYMLLYSRCLPRAPRLLRH   A DSKTR
Sbjct: 841  VCYVKNAKNKWFKIDDSTVTPVDIETVLTKGAYMLLYSRCLPRAPRLLRHSTVAVDSKTR 900

Query: 901  TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
             THSRTDERNT AKTTTTST QSYT+DTPGEPGSIGSIHFGHPQI RIFEEDSSSD+SSL
Sbjct: 901  ATHSRTDERNTAAKTTTTSTRQSYTRDTPGEPGSIGSIHFGHPQIHRIFEEDSSSDHSSL 960

Query: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLT----PLHL 1010
            ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQN P+L     PLHL
Sbjct: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNFPNLNPHVHPLHL 977

BLAST of Lcy12g015350 vs. ExPASy TrEMBL
Match: A0A6J1BV65 (ubiquitin carboxyl-terminal hydrolase 16-like OS=Momordica charantia OX=3673 GN=LOC111005823 PE=3 SV=1)

HSP 1 Score: 1674.8 bits (4336), Expect = 0.0e+00
Identity = 870/1015 (85.71%), Postives = 915/1015 (90.15%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSG+IGLQVL VFV+FPVICLVIRRKWRLSVARKEEINRLLVLSSEEA+RAELEASAG
Sbjct: 1    MLVSGNIGLQVLFVFVIFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAYRAELEASAG 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y SSSFTPPGHQCAVCY PTTTRCARCKAVRYCSGKCQIIHWRQGHK+NCHPPSISN+ +
Sbjct: 61   YTSSSFTPPGHQCAVCYCPTTTRCARCKAVRYCSGKCQIIHWRQGHKDNCHPPSISNQAI 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            ASRTDFEQK+TEQDHYESSTSDSS  SFSGFSTSNI  ESSDDTSTSD SSQ+EP+KFDG
Sbjct: 121  ASRTDFEQKLTEQDHYESSTSDSSIASFSGFSTSNISGESSDDTSTSDGSSQIEPEKFDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            YMSAD TPDSLEITRGID+ADQTKPLSP FGNLVDS+DSF  SSKLNQLK SGSHGKDS 
Sbjct: 181  YMSADGTPDSLEITRGIDIADQTKPLSPKFGNLVDSMDSFLPSSKLNQLKSSGSHGKDSH 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLS--- 300
            SS+NSSSFVINGNHKDS +TVSSDFWART+ H+GST DSLG+   P LSGGHSRKLS   
Sbjct: 241  SSLNSSSFVINGNHKDSGSTVSSDFWARTLDHRGSTRDSLGESASPNLSGGHSRKLSNLQ 300

Query: 301  SPSSFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSA 360
            SP+ FSFNSSNVLQARS E+KNASNIT P A E KKDKKP++GENLSKNYS PSNVCNSA
Sbjct: 301  SPTPFSFNSSNVLQARSFEDKNASNITLPCAPEVKKDKKPLDGENLSKNYS-PSNVCNSA 360

Query: 361  PSNYQGHKKTGNF-KPASNSH-VRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKV 420
            PSN+QGHKKTGNF K  SNSH +RSRSTSSVRP NG GGK LDSNVS+SPPL+SERS+KV
Sbjct: 361  PSNHQGHKKTGNFTKSTSNSHGLRSRSTSSVRPLNGLGGKALDSNVSVSPPLRSERSSKV 420

Query: 421  VSGPIDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQ 480
            V GPIDTISKVQGKDGNFSQRK++S+CS+PSSAGGTSVSLSRIQSGRVD VQATSGIPSQ
Sbjct: 421  VGGPIDTISKVQGKDGNFSQRKIDSDCSLPSSAGGTSVSLSRIQSGRVDSVQATSGIPSQ 480

Query: 481  VACTLNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNW 540
            VA TLNSKNGL+TSMLKVVDQFRGSKLLKNN  CTVTEIPGRFNYKGLFSYDHFVKLYNW
Sbjct: 481  VASTLNSKNGLKTSMLKVVDQFRGSKLLKNNHTCTVTEIPGRFNYKGLFSYDHFVKLYNW 540

Query: 541  NKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQ 600
            NKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHS+SC               
Sbjct: 541  NKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSRSC--------------- 600

Query: 601  NLLIAPLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNG 660
                             KKGWCFNCEFE+LILKVKEGRSPLSPVGIISQLPSIGRQLGNG
Sbjct: 601  ----------------IKKGWCFNCEFENLILKVKEGRSPLSPVGIISQLPSIGRQLGNG 660

Query: 661  KEEDAHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRS 720
            KEEDAHEFLRYAIDTMQS+CCMESGV SSGSWEEETTLVGLTFGGY+ SKIKCTRCQ RS
Sbjct: 661  KEEDAHEFLRYAIDTMQSVCCMESGVKSSGSWEEETTLVGLTFGGYLFSKIKCTRCQSRS 720

Query: 721  ERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAP 780
            ERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLC RCK+YVRAKKKLKILEAP
Sbjct: 721  ERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCGRCKSYVRAKKKLKILEAP 780

Query: 781  NILTVVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSF 840
            NILTVVLKRFQSGNFGKLNKPIKFPEILD+ PFVRGTSDKS +YRLYGVVVHLDV+NSSF
Sbjct: 781  NILTVVLKRFQSGNFGKLNKPIKFPEILDLTPFVRGTSDKSLLYRLYGVVVHLDVLNSSF 840

Query: 841  SGHYVCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPD 900
            SGHYVCYVKN KNKWFKIDDSTVTPVD+E+VLTKGAYMLLYSRCLP+APRL+RHG+  PD
Sbjct: 841  SGHYVCYVKNVKNKWFKIDDSTVTPVDLETVLTKGAYMLLYSRCLPKAPRLIRHGIVTPD 900

Query: 901  SKTRTTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSD 960
             K R  HSRTDERN   KTTTTSTHQ Y K+ PGE GSIGS HFGHPQI RIFEEDSSSD
Sbjct: 901  PKAR-VHSRTDERNAATKTTTTSTHQLYVKEHPGEHGSIGSFHFGHPQIHRIFEEDSSSD 960

Query: 961  NSSLISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLTPLHLP 1011
            NSSLISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIP+LT  HLP
Sbjct: 961  NSSLISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPNLT--HLP 980

BLAST of Lcy12g015350 vs. ExPASy TrEMBL
Match: A0A1S3CQL2 (LOW QUALITY PROTEIN: ubiquitin carboxyl-terminal hydrolase 16-like OS=Cucumis melo OX=3656 GN=LOC103503663 PE=3 SV=1)

HSP 1 Score: 1654.8 bits (4284), Expect = 0.0e+00
Identity = 863/1014 (85.11%), Postives = 908/1014 (89.55%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSG+IGLQVL VFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASA 
Sbjct: 1    MLVSGNIGLQVLFVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAR 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y SSSFTP GHQCAVCYSPTTTRC+RCKAVRYCSGKCQIIHWRQGHKENCHPP   N  V
Sbjct: 61   YSSSSFTPLGHQCAVCYSPTTTRCSRCKAVRYCSGKCQIIHWRQGHKENCHPP---NHAV 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            AS TDFE KVTEQDHYESSTSDSS  SFSGFS+S + SESSD+TSTSDSSSQ+EP+K DG
Sbjct: 121  ASSTDFETKVTEQDHYESSTSDSSAASFSGFSSSILSSESSDETSTSDSSSQIEPEKSDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            YMSADA PDSLEITRG D+ADQ K L+PMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR
Sbjct: 181  YMSADAMPDSLEITRGTDIADQPKSLTPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
            SSINSSSFVI+GNHK+S ATVSSDFWART+ HKGS++DSL +  LPKLSGGH RKLSSP 
Sbjct: 241  SSINSSSFVIDGNHKESVATVSSDFWARTLDHKGSSTDSLSNSALPKLSGGHGRKLSSPP 300

Query: 301  SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
            SFSF+S+N+LQ   SE  NAS+ T    SEDKK KKPV+GEN SKN+SIPSNVCNSA SN
Sbjct: 301  SFSFSSTNILQC-GSEGTNASSTTLSSTSEDKKGKKPVDGENFSKNHSIPSNVCNSAASN 360

Query: 361  YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
             QGH+KTGNFKPAS SHV RSRSTS+VRPPNGSGGKTLDSNVS SPPL+ ERS KVV   
Sbjct: 361  NQGHRKTGNFKPASTSHVLRSRSTSAVRPPNGSGGKTLDSNVSTSPPLRCERSTKVV--- 420

Query: 421  IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
            +DTISKVQ KDGNFSQRK++SEC +PSSAGGTSVSLSRIQSGRVD +QATSGIPSQVACT
Sbjct: 421  VDTISKVQSKDGNFSQRKIDSECCVPSSAGGTSVSLSRIQSGRVDTMQATSGIPSQVACT 480

Query: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
            LNSKNGLRTSMLKVVDQFRGSKLLKN+ ACTV EIPGRFNYKGLFSYDHFVKLYNWNKLE
Sbjct: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNSHACTVAEIPGRFNYKGLFSYDHFVKLYNWNKLE 540

Query: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
            LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601  APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                         KKGWCFNCEFESL+LKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601  ------------IKKGWCFNCEFESLMLKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661  AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
            AHEFLRY ID+MQS+CCME GVSSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661  AHEFLRYVIDSMQSICCMEYGVSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721  RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
            RIMDLTVEIEGDIGRL+EALRKFTS EILDGDNKYLCSRCK+Y + KKKLKILEAPNILT
Sbjct: 721  RIMDLTVEIEGDIGRLDEALRKFTSKEILDGDNKYLCSRCKSYXKGKKKLKILEAPNILT 780

Query: 781  VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
            VVLKRFQSGNFGKLNKPIKFPEILD+APF+RGTSDKSP+YRLYGVVVHLDVMNSSFSGHY
Sbjct: 781  VVLKRFQSGNFGKLNKPIKFPEILDLAPFIRGTSDKSPIYRLYGVVVHLDVMNSSFSGHY 840

Query: 841  VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
            VCYVKNAKNKWFKIDDSTVTPVDIE+VLTKGAYMLLYSRCLPRAPRLLRHG  A DSKTR
Sbjct: 841  VCYVKNAKNKWFKIDDSTVTPVDIETVLTKGAYMLLYSRCLPRAPRLLRHGTVAVDSKTR 900

Query: 901  TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
              HSRTDERNT +KTTTTST QSYT+DTPGEPGSIGSIHFGHPQI RIFEEDSSSD+SSL
Sbjct: 901  AMHSRTDERNTASKTTTTSTRQSYTRDTPGEPGSIGSIHFGHPQIHRIFEEDSSSDHSSL 960

Query: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLT----PLHL 1010
            ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQN P L     PLHL
Sbjct: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNFPHLNPHVHPLHL 976

BLAST of Lcy12g015350 vs. ExPASy TrEMBL
Match: A0A6J1II51 (ubiquitin carboxyl-terminal hydrolase 16-like OS=Cucurbita maxima OX=3661 GN=LOC111477130 PE=3 SV=1)

HSP 1 Score: 1640.6 bits (4247), Expect = 0.0e+00
Identity = 860/1012 (84.98%), Postives = 906/1012 (89.53%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSGSIGLQVLLVFVVFPVI LVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG
Sbjct: 1    MLVSGSIGLQVLLVFVVFPVIYLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y  +S TP GHQCAVCYS TTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSIS+R V
Sbjct: 61   YTYTSITPLGHQCAVCYSTTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISDRPV 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            ASR DFE KVTEQDHYES TSDSS +SFSGFS+S I SESSDDTSTSDSSSQ+EP+KFDG
Sbjct: 121  ASRADFEHKVTEQDHYESFTSDSSASSFSGFSSSIISSESSDDTSTSDSSSQIEPEKFDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            Y+SADA PDSLE TRGID+ DQ KPLSPMFGNLV SVDSFPSS KLNQLKPSGSHGKDSR
Sbjct: 181  YISADAIPDSLETTRGIDIVDQPKPLSPMFGNLVGSVDSFPSSGKLNQLKPSGSHGKDSR 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
            SSINSSSFVI+GNHK S ATVSSDFWART+ HKG TSDSLG+ TLPK SGGH RKL S S
Sbjct: 241  SSINSSSFVIDGNHKVSGATVSSDFWARTLDHKGPTSDSLGNSTLPKFSGGHGRKLCSTS 300

Query: 301  SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
            SFSFNSSNVLQA SS+E +ASNIT PGASE KKDKKP++GENLSKNYSIPSNVCNSAPSN
Sbjct: 301  SFSFNSSNVLQACSSDETDASNITLPGASEYKKDKKPMDGENLSKNYSIPSNVCNSAPSN 360

Query: 361  YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
             QGHK TGN KPAS SHV RS+STSSVRP NGSGGKTLDSNVSISPPL++ERS KVV   
Sbjct: 361  SQGHKTTGNLKPASTSHVLRSKSTSSVRPRNGSGGKTLDSNVSISPPLRTERSTKVV--- 420

Query: 421  IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
            +DTISKVQ KDGNFSQRK++SE S+PSSAGGTSVSL RIQ GRVD VQATSG+P++VAC 
Sbjct: 421  VDTISKVQSKDGNFSQRKIDSEYSLPSSAGGTSVSLPRIQPGRVDSVQATSGVPAEVACN 480

Query: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
            LNSKNGLR SMLKVVDQFRGSK LKN+ ACTV+E+PGR NYKGLFSYDHFVKLYNWNKLE
Sbjct: 481  LNSKNGLRMSMLKVVDQFRGSK-LKNSHACTVSEMPGRLNYKGLFSYDHFVKLYNWNKLE 540

Query: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
            LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601  APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                         +KGWCFNCEFESLILKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601  ------------IRKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661  AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
            AHEFLRY IDTMQS+CCMESGVSSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661  AHEFLRYVIDTMQSICCMESGVSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721  RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
            RIMDLTVEIEGDIGRL+EALRKFTS EILDGDNKYLC RCK+YVRAKKKLKILEAPNILT
Sbjct: 721  RIMDLTVEIEGDIGRLDEALRKFTSKEILDGDNKYLCGRCKSYVRAKKKLKILEAPNILT 780

Query: 781  VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
            VV KRFQSGNFGKLNKPIKFPEILD+ PF+RGTSDKSP+YRLY VVVHLDVMNSSFSGHY
Sbjct: 781  VVFKRFQSGNFGKLNKPIKFPEILDLGPFIRGTSDKSPIYRLYAVVVHLDVMNSSFSGHY 840

Query: 841  VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
            VCYVK+AKNKWFKIDDSTVTPVDIE+VL KGAYMLLYSRCLPRAPRLL+HG  A DSKTR
Sbjct: 841  VCYVKSAKNKWFKIDDSTVTPVDIETVLMKGAYMLLYSRCLPRAPRLLQHGTVAVDSKTR 900

Query: 901  TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
             THSR DERNTTAKTT+TST QSY KDTPGEPG I SIH GH +I R+FEEDSSSD+SSL
Sbjct: 901  ATHSRIDERNTTAKTTSTSTRQSYPKDTPGEPGGIRSIHLGHREIHRLFEEDSSSDHSSL 960

Query: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLTP-LHLP 1011
            ISSTSDEGSYSTESTRDSTSADELSDYIFGDP RVWNT +NIPSL+P LHLP
Sbjct: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPSRVWNTSRNIPSLSPHLHLP 977

BLAST of Lcy12g015350 vs. ExPASy TrEMBL
Match: A0A6J1F836 (ubiquitin carboxyl-terminal hydrolase 16-like OS=Cucurbita moschata OX=3662 GN=LOC111443183 PE=3 SV=1)

HSP 1 Score: 1622.8 bits (4201), Expect = 0.0e+00
Identity = 847/998 (84.87%), Postives = 898/998 (89.98%), Query Frame = 0

Query: 1   MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
           MLVSGSIGLQVLLVFVVFPVI LVIRRKWRLSVARKEEINRLLVLSSEEAFRAEL+ASAG
Sbjct: 1   MLVSGSIGLQVLLVFVVFPVIYLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELDASAG 60

Query: 61  YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
           Y  +S TP GHQCAVCYS TTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSIS+R V
Sbjct: 61  YTYTSITPLGHQCAVCYSATTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISDRPV 120

Query: 121 ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
           ASR DFE KVTEQDHYES TSDSS +SFSGFS+S I SESSDDTSTSDSSSQ+EP+KFDG
Sbjct: 121 ASRADFEHKVTEQDHYESFTSDSSASSFSGFSSSIISSESSDDTSTSDSSSQIEPEKFDG 180

Query: 181 YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
           Y+SADA PDSLE TRGID+ DQ KPLSPMFGNLV SVDSFPSS KLNQLKPSGSHGKDSR
Sbjct: 181 YISADAIPDSLETTRGIDIVDQPKPLSPMFGNLVGSVDSFPSSGKLNQLKPSGSHGKDSR 240

Query: 241 SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
           SSINSSSFVI+GNH+ S ATVSSDFWART+ HKG TSDSLG+ TLPK SGGH RKL S S
Sbjct: 241 SSINSSSFVIDGNHRVSGATVSSDFWARTLDHKGPTSDSLGNSTLPKFSGGHGRKLHSTS 300

Query: 301 SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
           SFSFNSSNVLQA SS+E +ASNIT PGASE KKDKKP++GEN SK YSIPSNVCNSAPSN
Sbjct: 301 SFSFNSSNVLQACSSDETDASNITLPGASEYKKDKKPMDGENFSKTYSIPSNVCNSAPSN 360

Query: 361 YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
            QGHK TGN KPAS SHV RS+STSSVRP NGSGGKTLDSNVSISPPL+SERS+KVV   
Sbjct: 361 NQGHKNTGNLKPASTSHVLRSKSTSSVRPRNGSGGKTLDSNVSISPPLRSERSSKVV--- 420

Query: 421 IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
           +DTISKVQ KDGNFSQRK++SE S+PS+AGGTSVSLSRIQ GRVD VQATSG+P++VAC 
Sbjct: 421 VDTISKVQSKDGNFSQRKIDSEYSLPSNAGGTSVSLSRIQPGRVDSVQATSGVPAEVACN 480

Query: 481 LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
           LNSKNGLR SMLKVVDQFRGSK LKN+ ACTV+E+PGR NYKGLFSYDHFVKLYNWNKLE
Sbjct: 481 LNSKNGLRMSMLKVVDQFRGSK-LKNSHACTVSEMPGRLNYKGLFSYDHFVKLYNWNKLE 540

Query: 541 LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
           LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541 LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601 APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                        +KGWCFNCEFESLILKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601 ------------IRKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661 AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
           AHEFLRY IDTMQS+CCMESG+SSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661 AHEFLRYVIDTMQSICCMESGMSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721 RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
           RIMDLTVEIEGDIGRL+EALRKFTS EILDGDNKYLC RCK+YVRAKKKLKILEAPNILT
Sbjct: 721 RIMDLTVEIEGDIGRLDEALRKFTSKEILDGDNKYLCGRCKSYVRAKKKLKILEAPNILT 780

Query: 781 VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
           VV KRFQSGNFGKLNKPIKFPEILD+APF+RGTSDKSP+YRL+ VVVHLDVMNSSFSGHY
Sbjct: 781 VVFKRFQSGNFGKLNKPIKFPEILDLAPFIRGTSDKSPIYRLFAVVVHLDVMNSSFSGHY 840

Query: 841 VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
           VCYVK+AKNKWFKIDDSTVTPVDIE+VLTKGAYMLLYSRCLPRAPRLLRHG  A DSKTR
Sbjct: 841 VCYVKSAKNKWFKIDDSTVTPVDIETVLTKGAYMLLYSRCLPRAPRLLRHGTVAVDSKTR 900

Query: 901 TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
            THSR DERNTTAKTT+TST QSY+KDTPGEPGSI SIH+GH +  R+F+EDSSSD+SSL
Sbjct: 901 ATHSRIDERNTTAKTTSTSTRQSYSKDTPGEPGSIRSIHYGHRKFHRLFDEDSSSDHSSL 960

Query: 961 ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNT 998
           ISSTSDEGSYSTESTRDSTSADELSDYIFGDP RVWNT
Sbjct: 961 ISSTSDEGSYSTESTRDSTSADELSDYIFGDPSRVWNT 963

BLAST of Lcy12g015350 vs. NCBI nr
Match: XP_038897893.1 (ubiquitin carboxyl-terminal hydrolase 16-like [Benincasa hispida])

HSP 1 Score: 1697.6 bits (4395), Expect = 0.0e+00
Identity = 885/1014 (87.28%), Postives = 921/1014 (90.83%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSG+IGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLV SSEEAFRAELEASAG
Sbjct: 1    MLVSGNIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVASSEEAFRAELEASAG 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y SSSFTP GHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNR V
Sbjct: 61   YTSSSFTPLGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRAV 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            ASRTDFEQKVTEQDHYESSTSDSS  SFSGFS+S + SESSDDTSTSDSSSQ+EP+K DG
Sbjct: 121  ASRTDFEQKVTEQDHYESSTSDSSAASFSGFSSSILSSESSDDTSTSDSSSQIEPEKSDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            YMSADA PDSLEITRG D+ADQ KPL+PMFGNLVDSVDSFPSSSKLNQLKPSG HG+ SR
Sbjct: 181  YMSADAIPDSLEITRGTDIADQPKPLTPMFGNLVDSVDSFPSSSKLNQLKPSGGHGRHSR 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
            SSI+SSSFVI+GNHK+S  TV SDFWART+ HKGSTSDSLG   + K SGGH RKLSSPS
Sbjct: 241  SSIDSSSFVIDGNHKESGVTVPSDFWARTLDHKGSTSDSLGSSAVSKFSGGHGRKLSSPS 300

Query: 301  SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
            SFSFNSSNVLQA SSEEKNA NIT PG SEDKK KKPV+GENLSKNYSIP NVCNSA SN
Sbjct: 301  SFSFNSSNVLQACSSEEKNAPNITLPGTSEDKKGKKPVDGENLSKNYSIPLNVCNSADSN 360

Query: 361  YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
             QGHKKTGNFKPAS SHV RSRSTSSVRPPNGSGGKTLDSNVSISPPL+SERSNKVV   
Sbjct: 361  NQGHKKTGNFKPASTSHVIRSRSTSSVRPPNGSGGKTLDSNVSISPPLRSERSNKVV--- 420

Query: 421  IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
            +DTISKVQ KDGNFSQRK++SECS+PSSAGGTSVS SRIQSGRVD +QATSGI SQVACT
Sbjct: 421  VDTISKVQSKDGNFSQRKIDSECSLPSSAGGTSVSHSRIQSGRVDTMQATSGIASQVACT 480

Query: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
            LNSKNGLRTSMLKVVDQFRGSKLLKN+ ACTVTEIPGR +YKGLFSYDHFVKLYNWNKLE
Sbjct: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNSHACTVTEIPGRVSYKGLFSYDHFVKLYNWNKLE 540

Query: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
            LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601  APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                         KKGWCFNCEFESLILKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601  ------------IKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661  AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
            AHEFLRY IDTMQS+CCMESGVSSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661  AHEFLRYVIDTMQSICCMESGVSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721  RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
            RIMDLTVEIEGDI RL+EALRKFTS EILDGDNKYLCSRCK+YVRAKKKLKILEAPNILT
Sbjct: 721  RIMDLTVEIEGDISRLDEALRKFTSQEILDGDNKYLCSRCKSYVRAKKKLKILEAPNILT 780

Query: 781  VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
            VVLKRFQSGNFGKLNKPIKFPEILD+APF+RGTSDKSPVYRLYGVVVHLDVMNSSFSGHY
Sbjct: 781  VVLKRFQSGNFGKLNKPIKFPEILDLAPFIRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840

Query: 841  VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
            VCYVKNAKNKWFKIDDSTVTPVDIE+VLTKGAYMLLYSRCLPRAPRLLRHG  A DSKTR
Sbjct: 841  VCYVKNAKNKWFKIDDSTVTPVDIETVLTKGAYMLLYSRCLPRAPRLLRHGTVAVDSKTR 900

Query: 901  TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
             THSRTDERNTTAKTT+TST QSY +D+PGE GS G IHFGHPQI RIFEEDSSSD+SSL
Sbjct: 901  ATHSRTDERNTTAKTTSTSTRQSYPRDSPGEHGSTGFIHFGHPQIHRIFEEDSSSDHSSL 960

Query: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLT----PLHL 1010
            +SSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWN WQNIPSL+    PLHL
Sbjct: 961  LSSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNAWQNIPSLSSHVHPLHL 980

BLAST of Lcy12g015350 vs. NCBI nr
Match: XP_011652577.2 (ubiquitin carboxyl-terminal hydrolase 16 [Cucumis sativus] >KGN60255.1 hypothetical protein Csa_002428 [Cucumis sativus])

HSP 1 Score: 1682.2 bits (4355), Expect = 0.0e+00
Identity = 876/1014 (86.39%), Postives = 916/1014 (90.34%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSG+IGLQVL VFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAE+EASA 
Sbjct: 1    MLVSGNIGLQVLFVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAEIEASAR 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y SSSFTP GHQCAVCYSPTTTRC+RCKAVRYCSGKCQIIHWRQGHKENCHPP   N  V
Sbjct: 61   YTSSSFTPLGHQCAVCYSPTTTRCSRCKAVRYCSGKCQIIHWRQGHKENCHPP---NHAV 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            AS TDFE KVTEQDHYESSTSDSS  SFSGFS+S + SESSDDTSTSDSSSQ+EP+K DG
Sbjct: 121  ASSTDFETKVTEQDHYESSTSDSSAASFSGFSSSILSSESSDDTSTSDSSSQIEPEKSDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            YMSADA PDSLEITRGID+ADQ K L+PMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR
Sbjct: 181  YMSADAMPDSLEITRGIDIADQPKSLTPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
            SS+NSSSFVI+GNHK+S ATVSSDFWART+ HKGST+DSL +  LPK SGGH RKLSSP 
Sbjct: 241  SSVNSSSFVIDGNHKESVATVSSDFWARTLDHKGSTTDSLSNSALPKFSGGHGRKLSSPP 300

Query: 301  SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
            SFSF+SSN+LQA SSE  NASNIT PG SEDKK KKPV+GEN SKN+SIPSNVCNSA SN
Sbjct: 301  SFSFSSSNILQACSSEGMNASNITLPGTSEDKKGKKPVDGENFSKNHSIPSNVCNSAASN 360

Query: 361  YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
             QGHKKTGNFKPAS SHV RSRSTS+VRPPNGSGGKTLDSNVS SPPL+ ERS KVV   
Sbjct: 361  NQGHKKTGNFKPASTSHVLRSRSTSAVRPPNGSGGKTLDSNVSTSPPLRCERSAKVV--- 420

Query: 421  IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
            +D+ISKVQ KDGNFSQRK++SEC +PSSAGGTSVSLSRIQSGRVD +QATSGIPSQVACT
Sbjct: 421  VDSISKVQSKDGNFSQRKIDSECCLPSSAGGTSVSLSRIQSGRVDTMQATSGIPSQVACT 480

Query: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
            LNSKNGLRTSMLKVVDQFRGSKLLKN+ ACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE
Sbjct: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNSHACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540

Query: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
            LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601  APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                         KKGWCFNCEFESLILKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601  ------------VKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661  AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
            AHEFLRY IDTMQS+CC E GVSSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661  AHEFLRYVIDTMQSICCKEYGVSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721  RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
            RIMDLTVEIEGDIGRL+EALRKFTS EILDGDNKYLCSRCK+YVRAKKKLKILEAPNILT
Sbjct: 721  RIMDLTVEIEGDIGRLDEALRKFTSKEILDGDNKYLCSRCKSYVRAKKKLKILEAPNILT 780

Query: 781  VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
            VVLKRFQSGNFGKLNKPIKFPEILD+APF+RGTSDKSP+YRLYGVVVHLDVMNSSFSGHY
Sbjct: 781  VVLKRFQSGNFGKLNKPIKFPEILDLAPFIRGTSDKSPIYRLYGVVVHLDVMNSSFSGHY 840

Query: 841  VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
            VCYVKNAKNKWFKIDDSTVTPVDIE+VLTKGAYMLLYSRCLPRAPRLLRH   A DSKTR
Sbjct: 841  VCYVKNAKNKWFKIDDSTVTPVDIETVLTKGAYMLLYSRCLPRAPRLLRHSTVAVDSKTR 900

Query: 901  TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
             THSRTDERNT AKTTTTST QSYT+DTPGEPGSIGSIHFGHPQI RIFEEDSSSD+SSL
Sbjct: 901  ATHSRTDERNTAAKTTTTSTRQSYTRDTPGEPGSIGSIHFGHPQIHRIFEEDSSSDHSSL 960

Query: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLT----PLHL 1010
            ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQN P+L     PLHL
Sbjct: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNFPNLNPHVHPLHL 977

BLAST of Lcy12g015350 vs. NCBI nr
Match: XP_022133154.1 (ubiquitin carboxyl-terminal hydrolase 16-like [Momordica charantia])

HSP 1 Score: 1674.8 bits (4336), Expect = 0.0e+00
Identity = 870/1015 (85.71%), Postives = 915/1015 (90.15%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSG+IGLQVL VFV+FPVICLVIRRKWRLSVARKEEINRLLVLSSEEA+RAELEASAG
Sbjct: 1    MLVSGNIGLQVLFVFVIFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAYRAELEASAG 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y SSSFTPPGHQCAVCY PTTTRCARCKAVRYCSGKCQIIHWRQGHK+NCHPPSISN+ +
Sbjct: 61   YTSSSFTPPGHQCAVCYCPTTTRCARCKAVRYCSGKCQIIHWRQGHKDNCHPPSISNQAI 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            ASRTDFEQK+TEQDHYESSTSDSS  SFSGFSTSNI  ESSDDTSTSD SSQ+EP+KFDG
Sbjct: 121  ASRTDFEQKLTEQDHYESSTSDSSIASFSGFSTSNISGESSDDTSTSDGSSQIEPEKFDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            YMSAD TPDSLEITRGID+ADQTKPLSP FGNLVDS+DSF  SSKLNQLK SGSHGKDS 
Sbjct: 181  YMSADGTPDSLEITRGIDIADQTKPLSPKFGNLVDSMDSFLPSSKLNQLKSSGSHGKDSH 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLS--- 300
            SS+NSSSFVINGNHKDS +TVSSDFWART+ H+GST DSLG+   P LSGGHSRKLS   
Sbjct: 241  SSLNSSSFVINGNHKDSGSTVSSDFWARTLDHRGSTRDSLGESASPNLSGGHSRKLSNLQ 300

Query: 301  SPSSFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSA 360
            SP+ FSFNSSNVLQARS E+KNASNIT P A E KKDKKP++GENLSKNYS PSNVCNSA
Sbjct: 301  SPTPFSFNSSNVLQARSFEDKNASNITLPCAPEVKKDKKPLDGENLSKNYS-PSNVCNSA 360

Query: 361  PSNYQGHKKTGNF-KPASNSH-VRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKV 420
            PSN+QGHKKTGNF K  SNSH +RSRSTSSVRP NG GGK LDSNVS+SPPL+SERS+KV
Sbjct: 361  PSNHQGHKKTGNFTKSTSNSHGLRSRSTSSVRPLNGLGGKALDSNVSVSPPLRSERSSKV 420

Query: 421  VSGPIDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQ 480
            V GPIDTISKVQGKDGNFSQRK++S+CS+PSSAGGTSVSLSRIQSGRVD VQATSGIPSQ
Sbjct: 421  VGGPIDTISKVQGKDGNFSQRKIDSDCSLPSSAGGTSVSLSRIQSGRVDSVQATSGIPSQ 480

Query: 481  VACTLNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNW 540
            VA TLNSKNGL+TSMLKVVDQFRGSKLLKNN  CTVTEIPGRFNYKGLFSYDHFVKLYNW
Sbjct: 481  VASTLNSKNGLKTSMLKVVDQFRGSKLLKNNHTCTVTEIPGRFNYKGLFSYDHFVKLYNW 540

Query: 541  NKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQ 600
            NKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHS+SC               
Sbjct: 541  NKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSRSC--------------- 600

Query: 601  NLLIAPLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNG 660
                             KKGWCFNCEFE+LILKVKEGRSPLSPVGIISQLPSIGRQLGNG
Sbjct: 601  ----------------IKKGWCFNCEFENLILKVKEGRSPLSPVGIISQLPSIGRQLGNG 660

Query: 661  KEEDAHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRS 720
            KEEDAHEFLRYAIDTMQS+CCMESGV SSGSWEEETTLVGLTFGGY+ SKIKCTRCQ RS
Sbjct: 661  KEEDAHEFLRYAIDTMQSVCCMESGVKSSGSWEEETTLVGLTFGGYLFSKIKCTRCQSRS 720

Query: 721  ERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAP 780
            ERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLC RCK+YVRAKKKLKILEAP
Sbjct: 721  ERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCGRCKSYVRAKKKLKILEAP 780

Query: 781  NILTVVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSF 840
            NILTVVLKRFQSGNFGKLNKPIKFPEILD+ PFVRGTSDKS +YRLYGVVVHLDV+NSSF
Sbjct: 781  NILTVVLKRFQSGNFGKLNKPIKFPEILDLTPFVRGTSDKSLLYRLYGVVVHLDVLNSSF 840

Query: 841  SGHYVCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPD 900
            SGHYVCYVKN KNKWFKIDDSTVTPVD+E+VLTKGAYMLLYSRCLP+APRL+RHG+  PD
Sbjct: 841  SGHYVCYVKNVKNKWFKIDDSTVTPVDLETVLTKGAYMLLYSRCLPKAPRLIRHGIVTPD 900

Query: 901  SKTRTTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSD 960
             K R  HSRTDERN   KTTTTSTHQ Y K+ PGE GSIGS HFGHPQI RIFEEDSSSD
Sbjct: 901  PKAR-VHSRTDERNAATKTTTTSTHQLYVKEHPGEHGSIGSFHFGHPQIHRIFEEDSSSD 960

Query: 961  NSSLISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLTPLHLP 1011
            NSSLISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIP+LT  HLP
Sbjct: 961  NSSLISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPNLT--HLP 980

BLAST of Lcy12g015350 vs. NCBI nr
Match: XP_008466167.2 (PREDICTED: LOW QUALITY PROTEIN: ubiquitin carboxyl-terminal hydrolase 16-like [Cucumis melo])

HSP 1 Score: 1654.8 bits (4284), Expect = 0.0e+00
Identity = 863/1014 (85.11%), Postives = 908/1014 (89.55%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSG+IGLQVL VFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASA 
Sbjct: 1    MLVSGNIGLQVLFVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAR 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y SSSFTP GHQCAVCYSPTTTRC+RCKAVRYCSGKCQIIHWRQGHKENCHPP   N  V
Sbjct: 61   YSSSSFTPLGHQCAVCYSPTTTRCSRCKAVRYCSGKCQIIHWRQGHKENCHPP---NHAV 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            AS TDFE KVTEQDHYESSTSDSS  SFSGFS+S + SESSD+TSTSDSSSQ+EP+K DG
Sbjct: 121  ASSTDFETKVTEQDHYESSTSDSSAASFSGFSSSILSSESSDETSTSDSSSQIEPEKSDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            YMSADA PDSLEITRG D+ADQ K L+PMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR
Sbjct: 181  YMSADAMPDSLEITRGTDIADQPKSLTPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
            SSINSSSFVI+GNHK+S ATVSSDFWART+ HKGS++DSL +  LPKLSGGH RKLSSP 
Sbjct: 241  SSINSSSFVIDGNHKESVATVSSDFWARTLDHKGSSTDSLSNSALPKLSGGHGRKLSSPP 300

Query: 301  SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
            SFSF+S+N+LQ   SE  NAS+ T    SEDKK KKPV+GEN SKN+SIPSNVCNSA SN
Sbjct: 301  SFSFSSTNILQC-GSEGTNASSTTLSSTSEDKKGKKPVDGENFSKNHSIPSNVCNSAASN 360

Query: 361  YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
             QGH+KTGNFKPAS SHV RSRSTS+VRPPNGSGGKTLDSNVS SPPL+ ERS KVV   
Sbjct: 361  NQGHRKTGNFKPASTSHVLRSRSTSAVRPPNGSGGKTLDSNVSTSPPLRCERSTKVV--- 420

Query: 421  IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
            +DTISKVQ KDGNFSQRK++SEC +PSSAGGTSVSLSRIQSGRVD +QATSGIPSQVACT
Sbjct: 421  VDTISKVQSKDGNFSQRKIDSECCVPSSAGGTSVSLSRIQSGRVDTMQATSGIPSQVACT 480

Query: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
            LNSKNGLRTSMLKVVDQFRGSKLLKN+ ACTV EIPGRFNYKGLFSYDHFVKLYNWNKLE
Sbjct: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNSHACTVAEIPGRFNYKGLFSYDHFVKLYNWNKLE 540

Query: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
            LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601  APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                         KKGWCFNCEFESL+LKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601  ------------IKKGWCFNCEFESLMLKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661  AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
            AHEFLRY ID+MQS+CCME GVSSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661  AHEFLRYVIDSMQSICCMEYGVSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721  RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
            RIMDLTVEIEGDIGRL+EALRKFTS EILDGDNKYLCSRCK+Y + KKKLKILEAPNILT
Sbjct: 721  RIMDLTVEIEGDIGRLDEALRKFTSKEILDGDNKYLCSRCKSYXKGKKKLKILEAPNILT 780

Query: 781  VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
            VVLKRFQSGNFGKLNKPIKFPEILD+APF+RGTSDKSP+YRLYGVVVHLDVMNSSFSGHY
Sbjct: 781  VVLKRFQSGNFGKLNKPIKFPEILDLAPFIRGTSDKSPIYRLYGVVVHLDVMNSSFSGHY 840

Query: 841  VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
            VCYVKNAKNKWFKIDDSTVTPVDIE+VLTKGAYMLLYSRCLPRAPRLLRHG  A DSKTR
Sbjct: 841  VCYVKNAKNKWFKIDDSTVTPVDIETVLTKGAYMLLYSRCLPRAPRLLRHGTVAVDSKTR 900

Query: 901  TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
              HSRTDERNT +KTTTTST QSYT+DTPGEPGSIGSIHFGHPQI RIFEEDSSSD+SSL
Sbjct: 901  AMHSRTDERNTASKTTTTSTRQSYTRDTPGEPGSIGSIHFGHPQIHRIFEEDSSSDHSSL 960

Query: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLT----PLHL 1010
            ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQN P L     PLHL
Sbjct: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNFPHLNPHVHPLHL 976

BLAST of Lcy12g015350 vs. NCBI nr
Match: XP_022976891.1 (ubiquitin carboxyl-terminal hydrolase 16-like [Cucurbita maxima])

HSP 1 Score: 1640.6 bits (4247), Expect = 0.0e+00
Identity = 860/1012 (84.98%), Postives = 906/1012 (89.53%), Query Frame = 0

Query: 1    MLVSGSIGLQVLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60
            MLVSGSIGLQVLLVFVVFPVI LVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG
Sbjct: 1    MLVSGSIGLQVLLVFVVFPVIYLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEASAG 60

Query: 61   YISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTV 120
            Y  +S TP GHQCAVCYS TTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSIS+R V
Sbjct: 61   YTYTSITPLGHQCAVCYSTTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISDRPV 120

Query: 121  ASRTDFEQKVTEQDHYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDG 180
            ASR DFE KVTEQDHYES TSDSS +SFSGFS+S I SESSDDTSTSDSSSQ+EP+KFDG
Sbjct: 121  ASRADFEHKVTEQDHYESFTSDSSASSFSGFSSSIISSESSDDTSTSDSSSQIEPEKFDG 180

Query: 181  YMSADATPDSLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSR 240
            Y+SADA PDSLE TRGID+ DQ KPLSPMFGNLV SVDSFPSS KLNQLKPSGSHGKDSR
Sbjct: 181  YISADAIPDSLETTRGIDIVDQPKPLSPMFGNLVGSVDSFPSSGKLNQLKPSGSHGKDSR 240

Query: 241  SSINSSSFVINGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPS 300
            SSINSSSFVI+GNHK S ATVSSDFWART+ HKG TSDSLG+ TLPK SGGH RKL S S
Sbjct: 241  SSINSSSFVIDGNHKVSGATVSSDFWARTLDHKGPTSDSLGNSTLPKFSGGHGRKLCSTS 300

Query: 301  SFSFNSSNVLQARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSN 360
            SFSFNSSNVLQA SS+E +ASNIT PGASE KKDKKP++GENLSKNYSIPSNVCNSAPSN
Sbjct: 301  SFSFNSSNVLQACSSDETDASNITLPGASEYKKDKKPMDGENLSKNYSIPSNVCNSAPSN 360

Query: 361  YQGHKKTGNFKPASNSHV-RSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGP 420
             QGHK TGN KPAS SHV RS+STSSVRP NGSGGKTLDSNVSISPPL++ERS KVV   
Sbjct: 361  SQGHKTTGNLKPASTSHVLRSKSTSSVRPRNGSGGKTLDSNVSISPPLRTERSTKVV--- 420

Query: 421  IDTISKVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACT 480
            +DTISKVQ KDGNFSQRK++SE S+PSSAGGTSVSL RIQ GRVD VQATSG+P++VAC 
Sbjct: 421  VDTISKVQSKDGNFSQRKIDSEYSLPSSAGGTSVSLPRIQPGRVDSVQATSGVPAEVACN 480

Query: 481  LNSKNGLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLE 540
            LNSKNGLR SMLKVVDQFRGSK LKN+ ACTV+E+PGR NYKGLFSYDHFVKLYNWNKLE
Sbjct: 481  LNSKNGLRMSMLKVVDQFRGSK-LKNSHACTVSEMPGRLNYKGLFSYDHFVKLYNWNKLE 540

Query: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLI 600
            LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC                   
Sbjct: 541  LQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSC------------------- 600

Query: 601  APLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEED 660
                         +KGWCFNCEFESLILKVKEGRSPLSPVGIISQLP+IGRQLGNGKEED
Sbjct: 601  ------------IRKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPNIGRQLGNGKEED 660

Query: 661  AHEFLRYAIDTMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQE 720
            AHEFLRY IDTMQS+CCMESGVSSSGSWEEETTLVGLTFGGY+LSKIKCTRCQGRSERQE
Sbjct: 661  AHEFLRYVIDTMQSICCMESGVSSSGSWEEETTLVGLTFGGYLLSKIKCTRCQGRSERQE 720

Query: 721  RIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILT 780
            RIMDLTVEIEGDIGRL+EALRKFTS EILDGDNKYLC RCK+YVRAKKKLKILEAPNILT
Sbjct: 721  RIMDLTVEIEGDIGRLDEALRKFTSKEILDGDNKYLCGRCKSYVRAKKKLKILEAPNILT 780

Query: 781  VVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHY 840
            VV KRFQSGNFGKLNKPIKFPEILD+ PF+RGTSDKSP+YRLY VVVHLDVMNSSFSGHY
Sbjct: 781  VVFKRFQSGNFGKLNKPIKFPEILDLGPFIRGTSDKSPIYRLYAVVVHLDVMNSSFSGHY 840

Query: 841  VCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTR 900
            VCYVK+AKNKWFKIDDSTVTPVDIE+VL KGAYMLLYSRCLPRAPRLL+HG  A DSKTR
Sbjct: 841  VCYVKSAKNKWFKIDDSTVTPVDIETVLMKGAYMLLYSRCLPRAPRLLQHGTVAVDSKTR 900

Query: 901  TTHSRTDERNTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSL 960
             THSR DERNTTAKTT+TST QSY KDTPGEPG I SIH GH +I R+FEEDSSSD+SSL
Sbjct: 901  ATHSRIDERNTTAKTTSTSTRQSYPKDTPGEPGGIRSIHLGHREIHRLFEEDSSSDHSSL 960

Query: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPGRVWNTWQNIPSLTP-LHLP 1011
            ISSTSDEGSYSTESTRDSTSADELSDYIFGDP RVWNT +NIPSL+P LHLP
Sbjct: 961  ISSTSDEGSYSTESTRDSTSADELSDYIFGDPSRVWNTSRNIPSLSPHLHLP 977

BLAST of Lcy12g015350 vs. TAIR 10
Match: AT4G24560.1 (ubiquitin-specific protease 16 )

HSP 1 Score: 622.5 bits (1604), Expect = 6.1e-178
Identity = 432/1050 (41.14%), Postives = 595/1050 (56.67%), Query Frame = 0

Query: 1   MLVSGSIGLQ--VLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAELEAS 60
           ML+   +G+   VL+V +V P+I L +R KWR++  R+EEI RLL+ +SEEA RAELEAS
Sbjct: 1   MLLVLDLGISSLVLVVSLVLPLIGLFVRHKWRVAAQRREEIRRLLIHASEEAARAELEAS 60

Query: 61  AGYISSSFTPPGHQCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSI--- 120
             + S + +   H C VCY   TTRC+RCKAVRYCSGKCQIIHWRQGHK+ CHP SI   
Sbjct: 61  VEFSSVAVSNVFH-CPVCYCLATTRCSRCKAVRYCSGKCQIIHWRQGHKDECHPASIVYD 120

Query: 121 ----------------------------------------SNRTVASRTDFEQKVTEQ-- 180
                                                   SNR   S  D    + +   
Sbjct: 121 SEDESDSDLRLGEENGQNTPEETLLVGPEPVTIPIGESLLSNR-ARSPEDGNGDIADNKD 180

Query: 181 ---DHYES-STSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDGYMSADATPD 240
              D  E+ S +++S +SFSGFS+S   ++S D+ S  +S S  E ++ +  + A  + +
Sbjct: 181 DLIDKEEAVSVAETSGSSFSGFSSSP-RNDSGDEISRCESFSSSESERSESLLDAHVSVE 240

Query: 241 SLEITRGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSRSSINSSSFV 300
             +          +K LSP F +LV+SVD+  +  KL+  KP    G++   S +  S V
Sbjct: 241 PEDTCFSTIEDAPSKLLSPKFVHLVESVDNLANLPKLSVHKPEDDAGQNQSQSRSLHSLV 300

Query: 301 INGNHKDSDATV-SSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPSSFSFNSSN 360
            + +   +D ++ SSDFW   +      SDS        +     R  +S   FSF S  
Sbjct: 301 TDRHPVSADPSLKSSDFWGTALGSAERVSDSC-------VKSKSGRPGNSSLHFSFGSG- 360

Query: 361 VLQARSSEEKNASNITPPGASEDKKDKKPV----NGENLSKNYSIPSNVCNSAPSNYQGH 420
                SS + +A+ ++   +S  K+  +      +G NL +                   
Sbjct: 361 -----SSRDTSAAKVSEQRSSILKEAPRGTGYISDGVNLRE------------------- 420

Query: 421 KKTGNFKPASNSHVRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGPIDTIS 480
           +    F  A  +   S ST ++ P + S      S+V++     +   N  +  P+  + 
Sbjct: 421 RNAKRFDEAEIALPISSSTDALSPLDSSN----LSHVTLPKSKSASSENGSMLAPL-KVG 480

Query: 481 KVQGKDGNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACTLNSKN 540
           +VQ      S  K  ++    S  G  SV        RV   Q  +G   Q   +L+ ++
Sbjct: 481 EVQLLASKASNTKKCADLMKHSPLGAKSV--------RVLDHQKQNGAVVQHINSLHGRS 540

Query: 541 GLRTSMLKVVDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLELQPAG 600
           GL+ S+LKVVDQ+   K        +  E+ GR  +KGLF Y+ F KLY + K+E QP G
Sbjct: 541 GLKASVLKVVDQWTRPK--------SENEMAGRHGHKGLFPYEVFAKLYTY-KIEFQPCG 600

Query: 601 LINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLIAPLYI 660
           LIN GNSC+ANVV QCL FTPPLT YFLQ  HS++C                        
Sbjct: 601 LINVGNSCFANVVFQCLMFTPPLTTYFLQQFHSRAC------------------------ 660

Query: 661 YIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEEDAHEFL 720
                  TKK  CF C FE L++K KE +SPLSP G++SQL +IG  LGNGKEEDAHEFL
Sbjct: 661 -------TKKEQCFTCGFEKLVVKAKEEKSPLSPNGLLSQLQNIGIFLGNGKEEDAHEFL 720

Query: 721 RYAIDTMQSLCCMESGVSSSGSWE-EETTLVGLTFGGYILSKIKCTRCQGRSERQERIMD 780
           R+ +DTMQS+C   S    + S + E+TTL+GLTFGGY+ SKIKC +CQ +SE +E++MD
Sbjct: 721 RFVVDTMQSVCIKASEYDMTKSSKLEDTTLIGLTFGGYLRSKIKCMKCQVKSELREKMMD 780

Query: 781 LTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILTVVLK 840
           LTVEI+GDI  L++ALR+FT TEILDG+NKY C  CK+Y RAKKKLKI E PN+LT+ LK
Sbjct: 781 LTVEIDGDISTLDDALRRFTRTEILDGENKYRCGSCKSYERAKKKLKITEPPNVLTIALK 840

Query: 841 RFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHYVCYV 900
           RFQ+G FGKLNK I+FPE LD+AP+V G S+KS  Y+LYGV+VHLDVMN++FSGHYVCY+
Sbjct: 841 RFQAGKFGKLNKLIRFPETLDLAPYVSGGSEKSHDYKLYGVIVHLDVMNAAFSGHYVCYI 900

Query: 901 KNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRL-LRHGVGAPDSKTRTTH 960
           +N +NKW+K DDSTV   D+E +LTKGAYML Y+RC P  PRL +     A + K+R   
Sbjct: 901 RN-QNKWYKADDSTVVTSDVERILTKGAYMLFYARCTPTPPRLAVCTKTEASNKKSRVPL 956

Query: 961 SRTDERNTTAKTTTTSTHQSYTKDTP--GEPGSIGSIHFGHPQILRIFEEDSSSDNSSLI 991
            + +E++T +++ +TS+ +  + +TP  G  G+I S +    ++ +I EEDS+SD+SSL 
Sbjct: 961 PKANEKSTISRSVSTSSPE-LSSNTPGGGRSGNIQSFYSSFQRLQKILEEDSASDSSSLF 956

BLAST of Lcy12g015350 vs. TAIR 10
Match: AT5G65450.1 (ubiquitin-specific protease 17 )

HSP 1 Score: 471.9 bits (1213), Expect = 1.3e-132
Identity = 363/987 (36.78%), Postives = 490/987 (49.65%), Query Frame = 0

Query: 20  VICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAE--LEASAGYISSSFTPPGHQCAVCY 79
           ++ L+IRR+WR +  R+EE+ RL+ L++EE++ AE    A+  Y   S +   ++CAVC 
Sbjct: 3   LVFLLIRRQWRSASVRREEVIRLIALATEESYLAEEVRPATVDYGGDSVSDV-YRCAVCL 62

Query: 80  SPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTVASRTDFEQKVTEQDHYE 139
            PTTTRC++CK+VRYCS KCQI+HWR+GHKE C  P           D E++   Q  Y+
Sbjct: 63  YPTTTRCSQCKSVRYCSSKCQILHWRRGHKEECRSPDY---------DEEKEEYVQSDYD 122

Query: 140 SSTS--DSSTTSFSGFSTSNIGSESSDDTSTS-DSSSQVEPDKFDGYMSADATPDSLEIT 199
           +  S  D  +   +  S+SN+  + + D STS  S  +V+P             ++++ T
Sbjct: 123 AKESNVDFPSRGTAYESSSNVSVDVACDMSTSRPSIHKVQP-----------RSEAVDFT 182

Query: 200 RGIDVAD---QTKPLS-PMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSRSSINSSSFVI 259
             +++ D   +T+PLS     N  D V+S  + S                          
Sbjct: 183 TSLNIKDNLYETRPLSRKKSRNRTDKVESASNYS-------------------------- 242

Query: 260 NGNHKDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPSSFSFNSSNVL 319
                                 KG T     D  L KL   +SR+    ++ S  S   L
Sbjct: 243 ----------------------KGKT-----DAKLRKLGNQNSRRSGDSANMSI-SDQFL 302

Query: 320 QARSSEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSNYQGHKKTGNF 379
                EE NA                        K+  I S                   
Sbjct: 303 SVGFEEEMNA-----------------------LKHERITS------------------- 362

Query: 380 KPASNSHVRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGPIDTISKVQGKD 439
           +P+S S   S S++ + P                                          
Sbjct: 363 EPSSASAAMSSSSTLLLP------------------------------------------ 422

Query: 440 GNFSQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACTLNSKNGLRTSM 499
                 K NS+  +  +                                  S +GL+TS+
Sbjct: 423 -----SKANSKPKVSQA----------------------------------SSSGLKTSV 482

Query: 500 LKVVDQFRGSKLLKNNQ-ACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLELQPAGLINCG 559
            KVV  FR  +  K +Q + ++ E+         FSY+ FVKLY  +++ELQP GL+N G
Sbjct: 483 QKVVQHFRPPQSSKKSQPSSSIDEMS--------FSYELFVKLY-CDRVELQPFGLVNLG 542

Query: 560 NSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLIAPLYIYIFSI 619
           NSCYAN VLQCLAFT PL +Y ++GLHSK+C                             
Sbjct: 543 NSCYANAVLQCLAFTRPLISYLIRGLHSKTC----------------------------- 602

Query: 620 SGTKKGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEEDAHEFLRYAID 679
              KK WCF CEFE LILK + G SPLSP+ I+S+L  IG+ LG GKEEDAHEFLR A+D
Sbjct: 603 --RKKSWCFVCEFEHLILKARGGESPLSPIKILSKLQKIGKHLGPGKEEDAHEFLRCAVD 662

Query: 680 TMQSLCCMESGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQERIMDLTVEIE 739
           TMQS+   E+   ++G + EETTLVGLTFGGY+ SKIKC  C  +SER E +MDLTVEI+
Sbjct: 663 TMQSVFLKEA--PAAGPFAEETTLVGLTFGGYLHSKIKCMACLHKSERPELMMDLTVEID 719

Query: 740 GDIGRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILTVVLKRFQSGN 799
           GDIG LEEAL +FT+ E+LDG+N+Y C RCK+Y +AKKKL ILE PNILTVVLKRFQS N
Sbjct: 723 GDIGSLEEALAQFTAYEVLDGENRYFCGRCKSYQKAKKKLMILEGPNILTVVLKRFQSDN 719

Query: 800 FGKLNKPIKFPEILDMAPFVRGTS-DKSPVYRLYGVVVHLDVMNSSFSGHYVCYVKNAKN 859
           FGKL+KPI FPE+LD++P++   +    PVY LY VVVHLD M++ FSGHYVCY+K    
Sbjct: 783 FGKLSKPIHFPELLDISPYMSDPNHGDHPVYSLYAVVVHLDAMSTLFSGHYVCYIKTLDG 719

Query: 860 KWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTRTTHSRTDER 919
            WFKIDDS V PV +E+VL +GAYMLLY+R  PR           P SK     S+   R
Sbjct: 843 DWFKIDDSNVFPVQLETVLLEGAYMLLYARDSPR-----------PVSKNGGRKSK-QRR 719

Query: 920 NTTAKTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSLISSTSDEGS 979
           N  A      + +   K   G+  S+       P++      D SS + S + S+SD  +
Sbjct: 903 NLAA----IPSRKGNKKQRDGDNNSL------LPRV------DWSSGSLSSMFSSSD--T 719

Query: 980 YSTESTRDSTSADELSDYIFGDPGRVW 996
            S+ ST+DS+  + LSDY+FG    VW
Sbjct: 963 TSSCSTKDSSGIENLSDYLFGGVEPVW 719

BLAST of Lcy12g015350 vs. TAIR 10
Match: AT1G17110.1 (ubiquitin-specific protease 15 )

HSP 1 Score: 366.7 bits (940), Expect = 6.0e-101
Identity = 319/1033 (30.88%), Postives = 441/1033 (42.69%), Query Frame = 0

Query: 11  VLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAE---------------- 70
           + LV VV PV+  ++  KW     ++   N L  +++EEA RAE                
Sbjct: 12  LFLVLVVLPVVAYILLGKWSNISEKRVRANLLAQMAAEEALRAETVVNADRGVRFESVAT 71

Query: 71  -----------LEASAGYISSSFTPPG-----------------------------HQCA 130
                      + A  G + + F                                 H CA
Sbjct: 72  ENRAQRTRTKTVSAGGGAVRAEFDAGARETVAEQRSDSVTATCGVTVVAPVNNNELHVCA 131

Query: 131 VCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTVASRTDFEQKVTEQD 190
            C+ P  TRC+RCK+VRYCSGKCQIIHWR  HK+ C P   S  + + R  FE+     D
Sbjct: 132 RCFGPAKTRCSRCKSVRYCSGKCQIIHWRVAHKDECVPVE-SCSSSSERVSFEKDSVLYD 191

Query: 191 HYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDGYMSADATPDSLEIT 250
           H   ST  S+ T+ +    +   S+SS D +                            +
Sbjct: 192 HGMDSTMYSNNTTQAAKGKT---SKSSVDFA----------------------------S 251

Query: 251 RGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSRSSINSSSFVINGNH 310
            GI   D T  ++      V        SSK N+     S  +DS +  +SS        
Sbjct: 252 LGISQNDITPQINTQGRKSVGK----QHSSKANR----ESCRRDSATVFDSS-------- 311

Query: 311 KDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPSSFSFNSSNVLQARS 370
            D  A+   D     I HK S  +S   +T P+      R     S+   N  + +    
Sbjct: 312 -DEAASAGGDNKTSHIKHK-SRGNSYAAETNPR------RHSVDSSAVQMNGQSFVSGMQ 371

Query: 371 SEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSNYQGHKKTGNFKPAS 430
              K+ +N+              V       N   PSN   +A     G  K+G      
Sbjct: 372 ESHKHENNL-------------GVRSSFGCPNTQYPSNGTRTATLPRTGINKSGEQSCTE 431

Query: 431 NSHVRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGPIDTISK-VQGKDGNF 490
            S                                         G +  +SK V+ KD   
Sbjct: 432 TSK---------------------------------------KGQVAAVSKTVRSKDTGI 491

Query: 491 SQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACTLNSKNGLRTSMLKV 550
           S+                            + + +T GI   +        GLR S  K 
Sbjct: 492 SEES--------------------------NGISSTMGIMKMM--------GLRNS-TKH 551

Query: 551 VDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLELQPAGLINCGNSCY 610
            D+++                    N K LF Y+ F+K +     +L P GL+NCGNSCY
Sbjct: 552 DDRYK--------------------NLKMLFPYEEFLKFFQCEVFDLSPRGLVNCGNSCY 611

Query: 611 ANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLIAPLYIYIFSISGTK 670
           AN VLQ L  T PL AY L+  HS+SC                               + 
Sbjct: 612 ANAVLQSLTCTKPLVAYLLRRSHSRSC-------------------------------SG 671

Query: 671 KGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEEDAHEFLRYAIDTMQS 730
           K WC  CE E  ++ ++E   PLS   I+S + SI  Q+G+G +EDAHEFLR  + +MQS
Sbjct: 672 KDWCLMCELEQHVMMLRESGGPLSASRILSHMRSINCQIGDGSQEDAHEFLRLLVASMQS 731

Query: 731 LCCME-SGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQERIMDLTVEIEGDI 790
           +C     G +      +ETTLV   FGG + SK+KC RC   SER E IMDLT+EI G +
Sbjct: 732 ICLERLGGETKVDPRLQETTLVQHMFGGRLRSKVKCLRCDHESERYENIMDLTLEIYGWV 791

Query: 791 GRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILTVVLKRFQSGNFGK 850
             L++AL +FT  E LDG+N Y CSRC  YVRA+K+L I EAPNILT+VLKRFQ G +GK
Sbjct: 792 ESLQDALTQFTRPEDLDGENMYRCSRCAGYVRARKELSIHEAPNILTIVLKRFQEGRYGK 821

Query: 851 LNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHYVCYVKNAKNKWFK 910
           +NK I FPE+LDM PF+  T D  P+Y LY V+VHLD +N+SFSGHY+ YVK+ +  W++
Sbjct: 852 INKCISFPEMLDMIPFMTRTGDVPPLYMLYAVIVHLDTLNASFSGHYISYVKDLRGNWYR 821

Query: 911 IDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTRTTHSRTDERNTTA 970
           IDDS + PV +  V+++GAYML Y R  PR P+   H   AP   ++  +   ++R    
Sbjct: 912 IDDSEIHPVPMTQVMSEGAYMLFYMRSYPR-PQRGEHNGKAPVHHSQPRNEMKEQRKPVN 821

Query: 971 KTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSLISSTSDEGSYSTE 986
           +    + H++                            +SSS   SL +S SDE S++TE
Sbjct: 972 RFKPRADHKN---------------------------TESSSSEWSLFTS-SDEASFTTE 821

BLAST of Lcy12g015350 vs. TAIR 10
Match: AT1G17110.2 (ubiquitin-specific protease 15 )

HSP 1 Score: 366.7 bits (940), Expect = 6.0e-101
Identity = 318/1033 (30.78%), Postives = 441/1033 (42.69%), Query Frame = 0

Query: 11  VLLVFVVFPVICLVIRRKWRLSVARKEEINRLLVLSSEEAFRAE---------------- 70
           + LV VV PV+  ++  KW     ++   N L  +++EEA RAE                
Sbjct: 12  LFLVLVVLPVVAYILLGKWSNISEKRVRANLLAQMAAEEALRAETVVNADRGVRFESVAT 71

Query: 71  -----------LEASAGYISSSFTPPG-----------------------------HQCA 130
                      + A  G + + F                                 H CA
Sbjct: 72  ENRAQRTRTKTVSAGGGAVRAEFDAGARETVAEQRSDSVTATCGVTVVAPVNNNELHVCA 131

Query: 131 VCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTVASRTDFEQKVTEQD 190
            C+ P  TRC+RCK+VRYCSGKCQIIHWR  HK+ C P   S  + + R  FE+     D
Sbjct: 132 RCFGPAKTRCSRCKSVRYCSGKCQIIHWRVAHKDECVPVE-SCSSSSERVSFEKDSVLYD 191

Query: 191 HYESSTSDSSTTSFSGFSTSNIGSESSDDTSTSDSSSQVEPDKFDGYMSADATPDSLEIT 250
           H   ST  S+ T+ +    +   S+SS D +                            +
Sbjct: 192 HGMDSTMYSNNTTQAAKGKT---SKSSVDFA----------------------------S 251

Query: 251 RGIDVADQTKPLSPMFGNLVDSVDSFPSSSKLNQLKPSGSHGKDSRSSINSSSFVINGNH 310
            GI   D T  ++      V        SSK N+     S  +DS +  +SS        
Sbjct: 252 LGISQNDITPQINTQGRKSVGK----QHSSKANR----ESCRRDSATVFDSS-------- 311

Query: 311 KDSDATVSSDFWARTIAHKGSTSDSLGDKTLPKLSGGHSRKLSSPSSFSFNSSNVLQARS 370
            D  A+   D     I HK S  +S   +T P+      R     S+   N  + +    
Sbjct: 312 -DEAASAGGDNKTSHIKHK-SRGNSYAAETNPR------RHSVDSSAVQMNGQSFVSGMQ 371

Query: 371 SEEKNASNITPPGASEDKKDKKPVNGENLSKNYSIPSNVCNSAPSNYQGHKKTGNFKPAS 430
              K+ +N+              V       N   PSN   +A     G  K+G      
Sbjct: 372 ESHKHENNL-------------GVRSSFGCPNTQYPSNGTRTATLPRTGINKSGEQSCTE 431

Query: 431 NSHVRSRSTSSVRPPNGSGGKTLDSNVSISPPLKSERSNKVVSGPIDTISK-VQGKDGNF 490
            S                                         G +  +SK V+ KD   
Sbjct: 432 TSK---------------------------------------KGQVAAVSKTVRSKDTGI 491

Query: 491 SQRKMNSECSIPSSAGGTSVSLSRIQSGRVDCVQATSGIPSQVACTLNSKNGLRTSMLKV 550
           S+                            + + +T GI   +    ++K+  R   LKV
Sbjct: 492 SEES--------------------------NGISSTMGIMKMMGLRNSTKHDDRYKNLKV 551

Query: 551 VDQFRGSKLLKNNQACTVTEIPGRFNYKGLFSYDHFVKLYNWNKLELQPAGLINCGNSCY 610
                 S L+                   LF Y+ F+K +     +L P GL+NCGNSCY
Sbjct: 552 ------SSLM-------------------LFPYEEFLKFFQCEVFDLSPRGLVNCGNSCY 611

Query: 611 ANVVLQCLAFTPPLTAYFLQGLHSKSCTFQITGSFSAVSNFQNLLIAPLYIYIFSISGTK 670
           AN VLQ L  T PL AY L+  HS+SC                               + 
Sbjct: 612 ANAVLQSLTCTKPLVAYLLRRSHSRSC-------------------------------SG 671

Query: 671 KGWCFNCEFESLILKVKEGRSPLSPVGIISQLPSIGRQLGNGKEEDAHEFLRYAIDTMQS 730
           K WC  CE E  ++ ++E   PLS   I+S + SI  Q+G+G +EDAHEFLR  + +MQS
Sbjct: 672 KDWCLMCELEQHVMMLRESGGPLSASRILSHMRSINCQIGDGSQEDAHEFLRLLVASMQS 731

Query: 731 LCCME-SGVSSSGSWEEETTLVGLTFGGYILSKIKCTRCQGRSERQERIMDLTVEIEGDI 790
           +C     G +      +ETTLV   FGG + SK+KC RC   SER E IMDLT+EI G +
Sbjct: 732 ICLERLGGETKVDPRLQETTLVQHMFGGRLRSKVKCLRCDHESERYENIMDLTLEIYGWV 791

Query: 791 GRLEEALRKFTSTEILDGDNKYLCSRCKTYVRAKKKLKILEAPNILTVVLKRFQSGNFGK 850
             L++AL +FT  E LDG+N Y CSRC  YVRA+K+L I EAPNILT+VLKRFQ G +GK
Sbjct: 792 ESLQDALTQFTRPEDLDGENMYRCSRCAGYVRARKELSIHEAPNILTIVLKRFQEGRYGK 825

Query: 851 LNKPIKFPEILDMAPFVRGTSDKSPVYRLYGVVVHLDVMNSSFSGHYVCYVKNAKNKWFK 910
           +NK I FPE+LDM PF+  T D  P+Y LY V+VHLD +N+SFSGHY+ YVK+ +  W++
Sbjct: 852 INKCISFPEMLDMIPFMTRTGDVPPLYMLYAVIVHLDTLNASFSGHYISYVKDLRGNWYR 825

Query: 911 IDDSTVTPVDIESVLTKGAYMLLYSRCLPRAPRLLRHGVGAPDSKTRTTHSRTDERNTTA 970
           IDDS + PV +  V+++GAYML Y R  PR P+   H   AP   ++  +   ++R    
Sbjct: 912 IDDSEIHPVPMTQVMSEGAYMLFYMRSYPR-PQRGEHNGKAPVHHSQPRNEMKEQRKPVN 825

Query: 971 KTTTTSTHQSYTKDTPGEPGSIGSIHFGHPQILRIFEEDSSSDNSSLISSTSDEGSYSTE 986
           +    + H++                            +SSS   SL +S SDE S++TE
Sbjct: 972 RFKPRADHKN---------------------------TESSSSEWSLFTS-SDEASFTTE 825

BLAST of Lcy12g015350 vs. TAIR 10
Match: AT2G24640.1 (ubiquitin-specific protease 19 )

HSP 1 Score: 365.2 bits (936), Expect = 1.7e-100
Identity = 183/386 (47.41%), Postives = 243/386 (62.95%), Query Frame = 0

Query: 523 LFSYDHFVKLYNWNKLELQPAGLINCGNSCYANVVLQCLAFTPPLTAYFLQGLHSKSCTF 582
           LF Y+ FV+ YNW++  + P GL NCGNSC+ANVVLQCL++T PL AY L+  H + C  
Sbjct: 154 LFPYESFVRYYNWDRPIMAPCGLTNCGNSCFANVVLQCLSWTRPLVAYLLERGHKREC-- 213

Query: 583 QITGSFSAVSNFQNLLIAPLYIYIFSISGTKKGWCFNCEFESLILKVKEGRSPLSPVGII 642
                                         +  WCF CEFE+ + +    R P SP+ II
Sbjct: 214 -----------------------------RRNDWCFLCEFENHLDRANYSRFPFSPMNII 273

Query: 643 SQLPSIGRQLGNGKEEDAHEFLRYAIDTMQSLCCME-SGVSSSGSWEEETTLVGLTFGGY 702
           S+LP+IG  LG G++EDAHE +R+AID MQS+C  E  G        +ETTL+   FGG 
Sbjct: 274 SRLPNIGGNLGYGRQEDAHELMRFAIDMMQSVCLDEFGGEKVVPPRAQETTLIQYIFGGL 333

Query: 703 ILSKIKCTRCQGRSERQERIMDLTVEIEGDIGRLEEALRKFTSTEILDGDNKYLCSRCKT 762
           + S+++CT C   S++ E +MDLTVEI GD   LEE L +FT+ E L GDN Y C RC  
Sbjct: 334 LQSQVQCTACSNVSDQYENMMDLTVEIHGDAVSLEECLDQFTAKEWLQGDNLYKCDRCDD 393

Query: 763 YVRAKKKLKILEAPNILTVVLKRFQSGNFGKLNKPIKFPEILDMAPFVRGTSDKSPVYRL 822
           YV+A K+L I  APNILT+ LKRFQ G FGKLNK I FPE  D+ P++ G  + S VY+L
Sbjct: 394 YVKACKRLSIRCAPNILTIALKRFQGGRFGKLNKRISFPETFDLGPYMSGGGEGSDVYKL 453

Query: 823 YGVVVHLDVMNSSFSGHYVCYVKNAKNKWFKIDDSTVTPVDIESVLTKGAYMLLYSRCLP 882
           Y V+VHLD++N+SF GHY+CYVK+ +  W++IDDS V  V++E VL++ AYMLLYSR  P
Sbjct: 454 YAVIVHLDMLNASFFGHYICYVKDFRGNWYRIDDSEVEKVELEDVLSQRAYMLLYSRVQP 507

Query: 883 RAPRLLRHGVGAPDSKTRTTHSRTDE 908
           R P  LR      + KT T ++ +++
Sbjct: 514 R-PSNLRSEESQDEKKTDTLNTESNQ 507

HSP 2 Score: 55.8 bits (133), Expect = 2.3e-07
Identity = 23/56 (41.07%), Postives = 33/56 (58.93%), Query Frame = 0

Query: 72  QCAVCYSPTTTRCARCKAVRYCSGKCQIIHWRQGHKENCHPPSISNRTVASRTDFE 128
           +C+VC   TT +C+RCK+VRYCS  CQ   W+ GHK  C     ++ +   R D +
Sbjct: 63  ECSVCGKATTKKCSRCKSVRYCSAACQTSDWKSGHKLKCKGFRSTDSSPVRRDDID 118