Lcy12g015310 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g015310
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Argininosuccinate lyase
Location	Chr12: 43296556 .. 43300476 (+)
RNA-Seq Expression	Lcy12g015310
Synteny	Lcy12g015310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGGTGTCTGGGGTTCTCAAAGCATCTCGCACCGCCGATGCGGCGCCGTCCGTCGCCGACTCGCCGGAGAACCGCACCGGCAAGGAGGTTAAGCTTTGGGGCGGTCGTTTCGAGGAGAGCGTCACCGATGCAGTCGAGCGATTCACCGAATCGATCTCCTACGATAAGCAGCTTTACAAGCACGATATCAGAGGAAGTCGAGCTCATGCTACTATGCTAGCTAAGCAGGTTCGTTTCCATTCCTCTACTTCTCTGCGTAAACAAAATCGCATTCGTTTTTCTCTTTCTGAGCGTTGCATTTTGGTCGACTTAACTACTGTTTGGCAGAAGAATAGAAATTAATTGACATCGCTGTTGTCAGGTAATTTCATCTTTTAATTTCTTTATTGAGAAATGTAATTCGAGCGGATACATTGATTTAAGGTTGCGGATTACATTTAAACTCCTTAATTTTGTTGTAGTTTACCAGGTGGCTTGTTGGTTGAATCTTTGTCCATCCTGGTTTAGATGAATTCAGAAATATATATCATACGGTGTTATTATATTCATGGATATTTTTCTTTTTGTGTCTTTAATGATTGGAAACTTAATACTTTAGGGACTGATGAGTGCTGATGAAAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAAGCGCATTGAAAGTGGTGATTTTGTTTGGAGAACAGATAGGGAGGATGTTCATATGAACATTGAGGCTGCCTTGATTGATATGATTGGTGAGCCTGCAAAGAAGCTACATACTGCTCGAAGCCGCAACGATCAAGTTCTGACTGATTTTCGATTGTGGTGTCGGGATGCAATTGATAATATATTAGATGCTATTAAGTACCTTCAGGTTTCCATGGTAACTCTGGCTTTGAAGAATGAGGGAGTAATCGTTCCGGGCTATACACATTTACAACGAGCGCAGCCCGTTCTGCTTCAGCATCTGCTCTTAGCATTTGTTGAGCAGGTAAGCTTCTTGAAGTGAAGTTCCTCTGTCTTCCTTGATTCAAGCCTAAGAGTTTTCAACTTATCAGGATAAGCAATTGGCAATATCTACATGCATGATTATTTTATATTCGTTGCAATTGATGCTAGCAGTGATGGGACTAACTCTAGCCTAAAAGAAATAGAAAGTTTTCAATGCTTGTACTTTTTAGGAATCAGGATTATGCCATCAGGATTTTCTTGAAATCTCATTCAATTTATTGACAGTGTTATTGATAGCTTGAGTGTTCATTATAACTTCATTTTTTTAAGTCAGAAACAGACATTTTTGTTAAAATAAAAGTACTTTTAGCTTATATAATATTCACATATGGTTGTATTTTTTTTAAAAGCCGTGTGAGCTCATGCAATCTCAAGTGCACATTAAGAACATTTGGATTTACATGTTGCCCCTTTGACTAATGGGCAAAACAATGGTGGTTATTTGATGTCACTTTGATTGAAAAATTAGTATTTTTTAATGATCAGTTGGCAAATGGATCAATAGTTTATCGTTAAATTTGCATAACAATCAACCTTACTCATCGTTTCTTAAATAAAATGTGGTTTTAAGCACTCTATTTTTTCTCTCTCTCTCAACTTAAAATCTCTAGAAGACTTGATTGTTGTAAAATCATTGTTTTATAGCTTGAAAGAGATGCTGGTCGTTTGTCGGATTGCAGAGTTAGGCTAAATCTTTGCCCGCTAGGTGCATGTGCGCTAGCCGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGAGGCTTTAGGGTTCACCGGTCCCTTGAGAAACAGGTATAAAACTTCATTTCTTTCTCTCCTTTATTTATTTATTTATTTTTATTTAAAAGCTGTAAGAGTTGCTAAAGATTGTTTTCCTGTTCGACTTGCTATTTAAAGTTATTCTTGAGATATGTGTGTATGTGTGGTAGTTTATCCGTACAAATAACAAAGGGTTTAGAGAGAATAGGTTCAAGCCATGGTGGCCACCTACCTAAGACTTAATATCTTATGAATTTCCTATTTACCTTGACAACCGAATGTAGTAGGGTCAAATGGTTATTCCATTAGAATAGTTAGGTGCGAGCAAGCTGGTTCAAACACTCACTATATATATATATACACGCACATGTGAATGTATATTTGCATATATAGCTTTTTTTTTGTCTCCTTCACTTCAATAAATAATTTTGGCCATAAAACAGCTTGGGAATTGACTTCTATATTTGGAAGTCAAAAGCTTTCACTTTCTGTGGGGAGTTTTAATGACTTCTATTTCCACGTCTGATTGTGTCAAATCATTAGTAATTTATCAGTATCTTTACAGCATTGATGCAGTTTCAGATCGAGATTTTGCCTTGGAGTTTCTTTCTGCCAATGCCATTACGGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAATTCGGATTTATATCGCCAAATGATTCCGTTTCAACTGGTAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTCGTTCGTGGAAAATCCGCTAGAGTCATAGGGGACTTGGTAACCACTCTCACTTTATGCAAAGGTCTGCCTCTGGCCTATAATCGTGATTTGCAGGTAAATCTTTGTGCATTTTGTTTTGACCAATTTGTTATATGCATAATAAAGGGTTGACACTTCCAAAGTGAATTCTGTAGACTTATACAACCCGAGTGTGTTTTTTCCATTTAGGAAATTTTAATTCCCAGACGAATGCTTCACTTTACTTTTTCTTAACAATGTCGTTTTTAGTTCTTGTTAATACAAAAACGTTACGATATTGAACAGGAAGACAAGGAACCTGTATTTGATAGCGTAAAAACGATATTGGGGATGCTGGAAGTGTCAGCTGAGTTTGCTCAGAATATTTCTTTCAATCGGGAGAGAATACAAAAGGCTTTGCCTGCTGGTTATCTTGATGCCACAACACTTGCCGATTACCTCGTGAAGAAGGTAACTAAATTCCTATTGGTTTGATTGCAATTTTTTTTTTTTTTTTTTTCAAAAACTGTCTTAGTTCTTGTGATTAATATAAGGTAAGCAACATCGATTTGTCTGTTATTGCACTACCTAGCATATTGATATTAGCCAAAGACGACCCCGTTTTAATACTGCTTAGGCGTTCGAGACCTCTATTGTAAAACTCTTTCCACTAGGGAAGTAAAAACAGAAAAAGAGAAACAAACATCGAGTTTTTTAAGGCCTCTTTATTGTGTAGAATCAAATTCTATACATTCAATGACATTTCAATTTTGACACCAGTGACATTTCAATTTGTCATTCCTTTTGCAGGGAATACCTTTCAGAACAGGTCATGATATAGTTGGGAAGTCTGTTGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGGGTCTGGATGAGCTAAGAAGCATAAGTCCAGTATTTGAAGAGGACGTTTACGAGTTTCTCGGCGCAGAGAACGCAATTAATAAATTTTGTTCATATGGATCGACAGGAGCAGCCTGTGTTGCTACTCAAATCGAATACTGGGTTAATAAATTCCAAATCAATCTGTAGACCTGGTTTGGATGCCATCTGGAAGTGGCCGTTTTCGAGTCGAAGATTCGGCAACATTTTGGGGGTATGATCGTAGGAAGGTACGGATAATTGTAGGTTGAAATTAGGATATATTATGGCGCCTCTGTGCAAGGAAGAAGTGTTTTATATGAATGTTTGTTGTCGAAGTTTTGATCTCTTTACTACTTCGATAGTTGGTTTCTCCAAACTTCAAAGAAATTAAATTATAGCCATGGATTTGAATGATGGTATGTAATTTTTGCTAGACATGTTTATTTACGAACTAATTATTTTGGTTTTAAGAGTGAACTGTCGAGAAAAATAGCATCCAAAGG

mRNA sequence

ATGCCGGTGTCTGGGGTTCTCAAAGCATCTCGCACCGCCGATGCGGCGCCGTCCGTCGCCGACTCGCCGGAGAACCGCACCGGCAAGGAGGTTAAGCTTTGGGGCGGTCGTTTCGAGGAGAGCGTCACCGATGCAGTCGAGCGATTCACCGAATCGATCTCCTACGATAAGCAGCTTTACAAGCACGATATCAGAGGAAGTCGAGCTCATGCTACTATGCTAGCTAAGCAGGGACTGATGAGTGCTGATGAAAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAAGCGCATTGAAAGTGGTGATTTTGTTTGGAGAACAGATAGGGAGGATGTTCATATGAACATTGAGGCTGCCTTGATTGATATGATTGGTGAGCCTGCAAAGAAGCTACATACTGCTCGAAGCCGCAACGATCAAGTTCTGACTGATTTTCGATTGTGGTGTCGGGATGCAATTGATAATATATTAGATGCTATTAAGTACCTTCAGGTTTCCATGGTAACTCTGGCTTTGAAGAATGAGGGAGTAATCGTTCCGGGCTATACACATTTACAACGAGCGCAGCCCGTTCTGCTTCAGCATCTGCTCTTAGCATTTGTTGAGCAGCTTGAAAGAGATGCTGGTCGTTTGTCGGATTGCAGAGTTAGGCTAAATCTTTGCCCGCTAGGTGCATGTGCGCTAGCCGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGAGGCTTTAGGGTTCACCGGTCCCTTGAGAAACAGCATTGATGCAGTTTCAGATCGAGATTTTGCCTTGGAGTTTCTTTCTGCCAATGCCATTACGGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAATTCGGATTTATATCGCCAAATGATTCCGTTTCAACTGGTAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTCGTTCGTGGAAAATCCGCTAGAGTCATAGGGGACTTGGTAACCACTCTCACTTTATGCAAAGGTCTGCCTCTGGCCTATAATCGTGATTTGCAGGTAAATCTTTGTGCATTTTGTTTTGACCAATTTGTTATATGCATAATAAAGGGTTGACACTTCCAAAGTGAATTCTGTAGACTTATACAACCCGAGTGTGTTTTTTCCATTTAGGAAATTTTAATTCCCAGACGAATGCTTCACTTTACTTTTTCTTAACAATGTCGTTTTTAGTTCTTGTTAATACAAAAACGTTACGATATTGAACAGGAAGACAAGGAACCTGTATTTGATAGCGTAAAAACGATATTGGGGATGCTGGAAGTGTCAGCTGAGTTTGCTCAGAATATTTCTTTCAATCGGGAGAGAATACAAAAGGCTTTGCCTGCTGGTTATCTTGATGCCACAACACTTGCCGATTACCTCGTGAAGAAGGGAATACCTTTCAGAACAGGTCATGATATAGTTGGGAAGTCTGTTGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGGGTCTGGATGAGCTAAGAAGCATAAGTCCAGTATTTGAAGAGGACGTTTACGAGTTTCTCGGCGCAGAGAACGCAATTAATAAATTTTGTTCATATGGATCGACAGGAGCAGCCTGTGTTGCTACTCAAATCGAATACTGGGTTAATAAATTCCAAATCAATCTGTAGACCTGGTTTGGATGCCATCTGGAAGTGGCCGTTTTCGAGTCGAAGATTCGGCAACATTTTGGGGGTATGATCGTAGGAAGGTACGGATAATTGTAGGTTGAAATTAGGATATATTATGGCGCCTCTGTGCAAGGAAGAAGTGTTTTATATGAATGTTTGTTGTCGAAGTTTTGATCTCTTTACTACTTCGATAGTTGGTTTCTCCAAACTTCAAAGAAATTAAATTATAGCCATGGATTTGAATGATGGTATGTAATTTTTGCTAGACATGTTTATTTACGAACTAATTATTTTGGTTTTAAGAGTGAACTGTCGAGAAAAATAGCATCCAAAGG

Coding sequence (CDS)

ATGCCGGTGTCTGGGGTTCTCAAAGCATCTCGCACCGCCGATGCGGCGCCGTCCGTCGCCGACTCGCCGGAGAACCGCACCGGCAAGGAGGTTAAGCTTTGGGGCGGTCGTTTCGAGGAGAGCGTCACCGATGCAGTCGAGCGATTCACCGAATCGATCTCCTACGATAAGCAGCTTTACAAGCACGATATCAGAGGAAGTCGAGCTCATGCTACTATGCTAGCTAAGCAGGGACTGATGAGTGCTGATGAAAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAAGCGCATTGAAAGTGGTGATTTTGTTTGGAGAACAGATAGGGAGGATGTTCATATGAACATTGAGGCTGCCTTGATTGATATGATTGGTGAGCCTGCAAAGAAGCTACATACTGCTCGAAGCCGCAACGATCAAGTTCTGACTGATTTTCGATTGTGGTGTCGGGATGCAATTGATAATATATTAGATGCTATTAAGTACCTTCAGGTTTCCATGGTAACTCTGGCTTTGAAGAATGAGGGAGTAATCGTTCCGGGCTATACACATTTACAACGAGCGCAGCCCGTTCTGCTTCAGCATCTGCTCTTAGCATTTGTTGAGCAGCTTGAAAGAGATGCTGGTCGTTTGTCGGATTGCAGAGTTAGGCTAAATCTTTGCCCGCTAGGTGCATGTGCGCTAGCCGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGAGGCTTTAGGGTTCACCGGTCCCTTGAGAAACAGCATTGATGCAGTTTCAGATCGAGATTTTGCCTTGGAGTTTCTTTCTGCCAATGCCATTACGGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAATTCGGATTTATATCGCCAAATGATTCCGTTTCAACTGGTAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTCGTTCGTGGAAAATCCGCTAGAGTCATAGGGGACTTGGTAACCACTCTCACTTTATGCAAAGGTCTGCCTCTGGCCTATAATCGTGATTTGCAGGTAAATCTTTGTGCATTTTGTTTTGACCAATTTGTTATATGCATAATAAAGGGTTGA

Protein sequence

MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQVNLCAFCFDQFVICIIKG

Homology

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BLAST of Lcy12g015310 vs. ExPASy Swiss-Prot
Match: Q9LEU8 (Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10920 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 8.6e-151
Identity = 263/328 (80.18%), Postives = 289/328 (88.11%), Query Frame = 0


Query: 24  ENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSAD 83
           ++   KEVKLWGGRFEESVT+ VE+FTESIS+DK LYK DI GS+AHA+MLA QGL++  
Sbjct: 51  DDTASKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDS 110

Query: 84  ERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVL 143
           ++DSIL GLD+IE++IE+  F WRTDREDVHMNIEAAL D+IGEPAKKLHTARSRNDQV 
Sbjct: 111 DKDSILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVA 170

Query: 144 TDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVE 203
           TDFRLWCRDAID I+  I+ LQ ++V LALKNE +IVPGYTHLQRAQPVLL H+LL FVE
Sbjct: 171 TDFRLWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVE 230

Query: 204 QLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDF 263
           QLERDAGR  DCR RLN  PLGACALAGTGLPIDRFMTA ALGFT P+RNSIDAVSDRDF
Sbjct: 231 QLERDAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDF 290

Query: 264 ALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPNDSVSTGSSIMPQKKNPDPMELVRG 323
            LEFL  NA T IHLSRLGEEWVLWASEEFGF++P+DSVSTGSSIMPQKKNPDPMELVRG
Sbjct: 291 VLEFLYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRG 350

Query: 324 KSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           KSARVIGDLVT LTLCKGLPLAYNRD Q
Sbjct: 351 KSARVIGDLVTVLTLCKGLPLAYNRDFQ 378

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BLAST of Lcy12g015310 vs. ExPASy Swiss-Prot
Match: B8GSV2 (Argininosuccinate lyase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=argH PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 6.7e-103
Identity = 187/320 (58.44%), Postives = 231/320 (72.19%), Query Frame = 0


Query: 32  KLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSADERDSILEG 91
           KLWGGRF E     VE FT S+S+D++LY+HDI+GS+AHA MLA+ G+++ +E D I +G
Sbjct: 9   KLWGGRFSEPTDAFVEAFTASVSFDQRLYRHDIQGSKAHARMLARVGVLTEEECDKIHDG 68

Query: 92  LDEIEKRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCR 151
           LD I   IE GDF W  + EDVHMN+EA LI+ IG+  KKLHT RSRNDQV TD RL+ R
Sbjct: 69  LDAILADIERGDFAWSVELEDVHMNVEARLIERIGDVGKKLHTGRSRNDQVATDIRLYLR 128

Query: 152 DAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGR 211
           +AID ++  I+ LQ  +V LA +    I+PG+THLQ AQPV   H ++A+ E LERD GR
Sbjct: 129 EAIDALMQEIRRLQGGLVDLAEREAQTIMPGFTHLQTAQPVTFGHHMMAWYEMLERDHGR 188

Query: 212 LSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFALEFLSAN 271
           L+DCR R+N+ PLGA ALAGT  P+DR  TAE LGF     NS+DAVSDRDFA+EF +A 
Sbjct: 189 LADCRKRVNVLPLGAAALAGTPYPLDRHYTAELLGFDAVTDNSLDAVSDRDFAIEFCAAG 248

Query: 272 AITAIHLSRLGEEWVLWASEEFGFISPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGD 331
           A+   HLSR  EE +LWAS +FGF+   D   TGSSIMPQKKNPD  ELVRGK+ R  G+
Sbjct: 249 ALIMTHLSRFSEELILWASAQFGFVDLPDRFCTGSSIMPQKKNPDVPELVRGKTGRANGN 308

Query: 332 LVTTLTLCKGLPLAYNRDLQ 352
           L+  LTL KG PLAYN+D Q
Sbjct: 309 LIALLTLMKGQPLAYNKDNQ 328

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BLAST of Lcy12g015310 vs. ExPASy Swiss-Prot
Match: Q2RXE3 (Argininosuccinate lyase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=argH PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-101
Identity = 184/319 (57.68%), Postives = 231/319 (72.41%), Query Frame = 0


Query: 33  LWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSADERDSILEGL 92
           +WGGRF+   +  +E    SI +DK+LY+ DI GS+AH TML   G++S  + ++IL GL
Sbjct: 18  IWGGRFDSGPSAVMEAINASIGFDKRLYRQDIAGSKAHCTMLVATGILSKADGEAILGGL 77

Query: 93  DEIEKRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCRD 152
           D I   IE+GDF +    ED+HMNIE+ L D+IGE A +LHTARSRNDQV TDFRLW RD
Sbjct: 78  DRILAEIEAGDFPFSVALEDIHMNIESRLKDLIGEAAGRLHTARSRNDQVATDFRLWVRD 137

Query: 153 AIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGRL 212
           AID +  A+  LQ  ++T A ++   ++PG+THLQ AQPV   H LLA+VE + RD GR 
Sbjct: 138 AIDGVEGALARLQDVLITRAEEHADTVMPGFTHLQAAQPVTFGHHLLAYVEMIGRDRGRF 197

Query: 213 SDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFALEFLSANA 272
            D RVRLN  PLG+ ALAGT  PIDR MTA+ LGF  P  NS+D VSDRDFALEFL+A +
Sbjct: 198 HDARVRLNESPLGSAALAGTSFPIDRAMTAQILGFDRPCANSLDGVSDRDFALEFLAAAS 257

Query: 273 ITAIHLSRLGEEWVLWASEEFGFISPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDL 332
           I +IHLSRL EE V+W S +FGF+   D+ STGSSIMPQK+NPD  ELVR K+ RVIGDL
Sbjct: 258 IASIHLSRLAEELVIWTSAQFGFVRLPDAYSTGSSIMPQKRNPDAAELVRAKAGRVIGDL 317

Query: 333 VTTLTLCKGLPLAYNRDLQ 352
            + L + KGLPLAY++D+Q
Sbjct: 318 ASLLIVMKGLPLAYSKDMQ 336

[Close]

BLAST of Lcy12g015310 vs. ExPASy Swiss-Prot
Match: B7H1L9 (Argininosuccinate lyase OS=Acinetobacter baumannii (strain AB307-0294) OX=557600 GN=argH PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.4e-100
Identity = 191/340 (56.18%), Postives = 232/340 (68.24%), Query Frame = 0


Query: 12  TADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA 71
           T  + P  + +P   +G    +WGGRF E+    V  FT S+ +D++ YK DI GS AHA
Sbjct: 2   TTSSNPPNSAAPNQTSG----MWGGRFSEATDAFVAEFTASVQFDQRFYKQDIAGSIAHA 61

Query: 72  TMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKK 131
           TMLAK G+++  ERD I+EGL  I   IE+G F WR D EDVHMNIE+ L   IG   KK
Sbjct: 62  TMLAKVGVLTEAERDDIIEGLSTIRAEIEAGTFEWRIDLEDVHMNIESRLTQRIGITGKK 121

Query: 132 LHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQP 191
           LHT RSRNDQV TD RL+ RD ID+IL  ++ LQ  ++ LA KN   I+PG+THLQ AQP
Sbjct: 122 LHTGRSRNDQVATDIRLYLRDEIDDILGLLERLQKGLLGLAAKNVNTIMPGFTHLQTAQP 181

Query: 192 VLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPL 251
           V   H LLA+ E L RD  RL DCR R+N  PLG+ ALAGT  PIDR  TAE LGF    
Sbjct: 182 VTFGHHLLAWFEMLVRDTERLQDCRKRVNRMPLGSAALAGTTYPIDRAYTAELLGFEAVS 241

Query: 252 RNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPNDSVSTGSSIMPQ 311
            NS+DAVSDRDFA+EF +A ++  +HLSR+ EE +LW S +F F++  D   TGSSIMPQ
Sbjct: 242 ENSLDAVSDRDFAIEFNAAASLIMMHLSRMSEELILWTSAQFKFVNIPDRFCTGSSIMPQ 301

Query: 312 KKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           KKNPD  EL+RGKS RV GDLV+ LTL KG PLAYN+D Q
Sbjct: 302 KKNPDVPELIRGKSGRVFGDLVSLLTLMKGQPLAYNKDNQ 337

[Close]

BLAST of Lcy12g015310 vs. ExPASy Swiss-Prot
Match: B7I338 (Argininosuccinate lyase OS=Acinetobacter baumannii (strain AB0057) OX=480119 GN=argH PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.4e-100
Identity = 191/340 (56.18%), Postives = 232/340 (68.24%), Query Frame = 0


Query: 12  TADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA 71
           T  + P  + +P   +G    +WGGRF E+    V  FT S+ +D++ YK DI GS AHA
Sbjct: 2   TTSSNPPNSAAPNQTSG----MWGGRFSEATDAFVAEFTASVQFDQRFYKQDIAGSIAHA 61

Query: 72  TMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKK 131
           TMLAK G+++  ERD I+EGL  I   IE+G F WR D EDVHMNIE+ L   IG   KK
Sbjct: 62  TMLAKVGVLTEAERDDIIEGLSTIRAEIEAGTFEWRIDLEDVHMNIESRLTQRIGITGKK 121

Query: 132 LHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQP 191
           LHT RSRNDQV TD RL+ RD ID+IL  ++ LQ  ++ LA KN   I+PG+THLQ AQP
Sbjct: 122 LHTGRSRNDQVATDIRLYLRDEIDDILGLLERLQKGLLGLAAKNVNTIMPGFTHLQTAQP 181

Query: 192 VLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPL 251
           V   H LLA+ E L RD  RL DCR R+N  PLG+ ALAGT  PIDR  TAE LGF    
Sbjct: 182 VTFGHHLLAWFEMLVRDTERLQDCRKRVNRMPLGSAALAGTTYPIDRAYTAELLGFEAVS 241

Query: 252 RNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPNDSVSTGSSIMPQ 311
            NS+DAVSDRDFA+EF +A ++  +HLSR+ EE +LW S +F F++  D   TGSSIMPQ
Sbjct: 242 ENSLDAVSDRDFAIEFNAAASLIMMHLSRMSEELILWTSAQFKFVNIPDRFCTGSSIMPQ 301

Query: 312 KKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           KKNPD  EL+RGKS RV GDLV+ LTL KG PLAYN+D Q
Sbjct: 302 KKNPDVPELIRGKSGRVFGDLVSLLTLMKGQPLAYNKDNQ 337

[Close]

BLAST of Lcy12g015310 vs. ExPASy TrEMBL
Match: A0A6J1CLI1 (argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111012263 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.3e-188
Identity = 334/351 (95.16%), Postives = 340/351 (96.87%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           MPV GVL ASRTADAAPSVADSP+N   KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 37  MPVFGVLSASRTADAAPSVADSPDNHADKEVKLWGGRFEESVTDAVERFTESISYDKQLY 96

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHA+MLAKQGLMS DERDSILEGLDEIE+RIESG+FVWRTDREDVHMNIEAA
Sbjct: 97  KHDIRGSRAHASMLAKQGLMSVDERDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAA 156

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAIKYLQVSMVTLAL NEG+IV
Sbjct: 157 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIV 216

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLN CPLGACALAGTGLPIDRFM
Sbjct: 217 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNYCPLGACALAGTGLPIDRFM 276

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TA+ALGFT PLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI PND
Sbjct: 277 TADALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPND 336

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 337 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 387

[Close]

BLAST of Lcy12g015310 vs. ExPASy TrEMBL
Match: A0A5A7T8Y2 (Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005690 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.2e-187
Identity = 334/351 (95.16%), Postives = 342/351 (97.44%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           MP+ GVL+AS TA+A PSV DSPENRT KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  MPMFGVLRASHTANAPPSVYDSPENRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHATMLAKQGLMS DERDSILEGLD+IE++IESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHATMLAKQGLMSVDERDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAIKYLQVSMVTLALKNEG+IV
Sbjct: 155 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI+PND
Sbjct: 275 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFITPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. ExPASy TrEMBL
Match: A0A1S3CQK7 (argininosuccinate lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503657 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.2e-187
Identity = 334/351 (95.16%), Postives = 342/351 (97.44%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           MP+ GVL+AS TA+A PSV DSPENRT KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  MPMFGVLRASHTANAPPSVYDSPENRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHATMLAKQGLMS DERDSILEGLD+IE++IESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHATMLAKQGLMSVDERDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAIKYLQVSMVTLALKNEG+IV
Sbjct: 155 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI+PND
Sbjct: 275 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFITPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. ExPASy TrEMBL
Match: A0A0A0LE21 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G891650 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.0e-186
Identity = 331/351 (94.30%), Postives = 341/351 (97.15%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           M + GVL+AS TA+A PSV DSPENRT KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  MSMVGVLRASHTANAPPSVDDSPENRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHATMLAKQGLMS DERDSILEGLD+IE++IESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHATMLAKQGLMSVDERDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LID+IGEPAKKLHTARSRNDQVLTDFRLWCRDAID+ILDAIKYLQVSMVTLALKNEG+IV
Sbjct: 155 LIDIIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDSILDAIKYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGF GPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI+PND
Sbjct: 275 TAEALGFAGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFITPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. ExPASy TrEMBL
Match: A0A6J1FFD9 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111443572 PE=3 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 8.5e-186
Identity = 330/351 (94.02%), Postives = 341/351 (97.15%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           +PV GVL+ASRTADA  SV DSPENR+ KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  LPVFGVLRASRTADAPLSVDDSPENRSDKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHA+MLA QGL+S  ERDSI+EGLDEIE+RIESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHASMLANQGLISVAERDSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAI+YLQVSMVTLALKNEG+IV
Sbjct: 155 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGFT PLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGF++PND
Sbjct: 275 TAEALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFVTPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. NCBI nr
Match: XP_038900244.1 (argininosuccinate lyase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 667.2 bits (1720), Expect = 8.4e-188
Identity = 334/351 (95.16%), Postives = 342/351 (97.44%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           +PV GVL+ASRTADA PSV DSPENRT KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  VPVFGVLRASRTADAPPSVEDSPENRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHATMLAKQGLMS  ERDSILEGLD+IE++IESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHATMLAKQGLMSVHERDSILEGLDKIERQIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDA+KYLQVSMVTLALKNEG+IV
Sbjct: 155 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDTILDAMKYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGFT PLRNSIDAVSDRDFALEFLSANAI AIHLSRLGEEWVLWASEEFGFI+PND
Sbjct: 275 TAEALGFTAPLRNSIDAVSDRDFALEFLSANAIAAIHLSRLGEEWVLWASEEFGFITPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. NCBI nr
Match: XP_022142042.1 (argininosuccinate lyase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 666.8 bits (1719), Expect = 1.1e-187
Identity = 334/351 (95.16%), Postives = 340/351 (96.87%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           MPV GVL ASRTADAAPSVADSP+N   KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 37  MPVFGVLSASRTADAAPSVADSPDNHADKEVKLWGGRFEESVTDAVERFTESISYDKQLY 96

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHA+MLAKQGLMS DERDSILEGLDEIE+RIESG+FVWRTDREDVHMNIEAA
Sbjct: 97  KHDIRGSRAHASMLAKQGLMSVDERDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAA 156

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAIKYLQVSMVTLAL NEG+IV
Sbjct: 157 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIV 216

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLN CPLGACALAGTGLPIDRFM
Sbjct: 217 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNYCPLGACALAGTGLPIDRFM 276

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TA+ALGFT PLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI PND
Sbjct: 277 TADALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPND 336

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 337 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 387

[Close]

BLAST of Lcy12g015310 vs. NCBI nr
Match: XP_008466162.1 (PREDICTED: argininosuccinate lyase, chloroplastic [Cucumis melo] >KAA0038666.1 argininosuccinate lyase [Cucumis melo var. makuwa] >TYK31276.1 argininosuccinate lyase [Cucumis melo var. makuwa])

HSP 1 Score: 665.6 bits (1716), Expect = 2.5e-187
Identity = 334/351 (95.16%), Postives = 342/351 (97.44%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           MP+ GVL+AS TA+A PSV DSPENRT KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  MPMFGVLRASHTANAPPSVYDSPENRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHATMLAKQGLMS DERDSILEGLD+IE++IESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHATMLAKQGLMSVDERDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAIKYLQVSMVTLALKNEG+IV
Sbjct: 155 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI+PND
Sbjct: 275 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFITPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. NCBI nr
Match: KAG6591697.1 (Argininosuccinate lyase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661.0 bits (1704), Expect = 6.0e-186
Identity = 332/351 (94.59%), Postives = 340/351 (96.87%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           +PV GVL+ASRTADA  SV DSPENR  KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  LPVFGVLRASRTADAPLSVDDSPENRADKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHA+MLA QGLMS  ERDSI+EGLDEIE+RIESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHASMLANQGLMSVAERDSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAI+YLQVSMVTLALKNEG+IV
Sbjct: 155 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGFT PLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI+PND
Sbjct: 275 TAEALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFITPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. NCBI nr
Match: XP_023536382.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 660.6 bits (1703), Expect = 7.9e-186
Identity = 332/351 (94.59%), Postives = 340/351 (96.87%), Query Frame = 0


Query: 1   MPVSGVLKASRTADAAPSVADSPENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLY 60
           +PV GVL+ASRTADA  SV DSPENR  KEVKLWGGRFEESVTDAVERFTESISYDKQLY
Sbjct: 35  LPVFGVLRASRTADAPLSVDDSPENRDDKEVKLWGGRFEESVTDAVERFTESISYDKQLY 94

Query: 61  KHDIRGSRAHATMLAKQGLMSADERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAA 120
           KHDIRGSRAHA+MLA QGLMS  ERDSI+EGLDEIE+RIESGDFVWRTDREDVHMNIEAA
Sbjct: 95  KHDIRGSRAHASMLASQGLMSVAERDSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAA 154

Query: 121 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIV 180
           LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAID ILDAI+YLQVSMVTLALKNEG+IV
Sbjct: 155 LIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIV 214

Query: 181 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 240
           PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM
Sbjct: 215 PGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFM 274

Query: 241 TAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPND 300
           TAEALGFT PLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFI+PND
Sbjct: 275 TAEALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFITPND 334

Query: 301 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ
Sbjct: 335 SVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQ 385

[Close]

BLAST of Lcy12g015310 vs. TAIR 10
Match: AT5G10920.1 (L-Aspartase-like family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 6.1e-152
Identity = 263/328 (80.18%), Postives = 289/328 (88.11%), Query Frame = 0


Query: 24  ENRTGKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSAD 83
           ++   KEVKLWGGRFEESVT+ VE+FTESIS+DK LYK DI GS+AHA+MLA QGL++  
Sbjct: 51  DDTASKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDS 110

Query: 84  ERDSILEGLDEIEKRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVL 143
           ++DSIL GLD+IE++IE+  F WRTDREDVHMNIEAAL D+IGEPAKKLHTARSRNDQV 
Sbjct: 111 DKDSILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVA 170

Query: 144 TDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVE 203
           TDFRLWCRDAID I+  I+ LQ ++V LALKNE +IVPGYTHLQRAQPVLL H+LL FVE
Sbjct: 171 TDFRLWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVE 230

Query: 204 QLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDF 263
           QLERDAGR  DCR RLN  PLGACALAGTGLPIDRFMTA ALGFT P+RNSIDAVSDRDF
Sbjct: 231 QLERDAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDF 290

Query: 264 ALEFLSANAITAIHLSRLGEEWVLWASEEFGFISPNDSVSTGSSIMPQKKNPDPMELVRG 323
            LEFL  NA T IHLSRLGEEWVLWASEEFGF++P+DSVSTGSSIMPQKKNPDPMELVRG
Sbjct: 291 VLEFLYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRG 350

Query: 324 KSARVIGDLVTTLTLCKGLPLAYNRDLQ 352
           KSARVIGDLVT LTLCKGLPLAYNRD Q
Sbjct: 351 KSARVIGDLVTVLTLCKGLPLAYNRDFQ 378

The following BLAST results are available for this feature:

BLAST of Lcy12g015310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LEU8	8.6e-151	80.18	Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10...	[more]
B8GSV2	6.7e-103	58.44	Argininosuccinate lyase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=3...	[more]
Q2RXE3	2.1e-101	57.68	Argininosuccinate lyase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 ...	[more]
B7H1L9	1.4e-100	56.18	Argininosuccinate lyase OS=Acinetobacter baumannii (strain AB307-0294) OX=557600...	[more]
B7I338	1.4e-100	56.18	Argininosuccinate lyase OS=Acinetobacter baumannii (strain AB0057) OX=480119 GN=...	[more]

BLAST of Lcy12g015310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLI1	5.3e-188	95.16	argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A5A7T8Y2	1.2e-187	95.16	Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CQK7	1.2e-187	95.16	argininosuccinate lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503657 P...	[more]
A0A0A0LE21	5.0e-186	94.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G891650 PE=3 SV=1	[more]
A0A6J1FFD9	8.5e-186	94.02	argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of Lcy12g015310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900244.1	8.4e-188	95.16	argininosuccinate lyase, chloroplastic-like [Benincasa hispida]	[more]
XP_022142042.1	1.1e-187	95.16	argininosuccinate lyase, chloroplastic-like [Momordica charantia]	[more]
XP_008466162.1	2.5e-187	95.16	PREDICTED: argininosuccinate lyase, chloroplastic [Cucumis melo] >KAA0038666.1 a...	[more]
KAG6591697.1	6.0e-186	94.59	Argininosuccinate lyase, chloroplastic, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023536382.1	7.9e-186	94.59	argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy12g015310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10920.1	6.1e-152	80.18	L-Aspartase-like family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00145	ARGSUCLYASE	coord: 131..153 score: 68.79 coord: 340..359 score: 47.43 coord: 261..285 score: 52.07 coord: 305..321 score: 81.13 coord: 223..239 score: 67.02 coord: 172..192 score: 58.77
None	No IPR available	GENE3D	1.20.200.10	Fumarase/aspartase (Central domain)	coord: 137..363 e-value: 1.0E-77 score: 263.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
IPR000362	Fumarate lyase family	PRINTS	PR00149	FUMRATELYASE	coord: 132..150 score: 36.91 coord: 305..321 score: 64.09 coord: 261..288 score: 27.87 coord: 177..195 score: 42.48
IPR009049	Argininosuccinate lyase	TIGRFAM	TIGR00838	TIGR00838	coord: 33..351 e-value: 3.5E-134 score: 446.2
IPR009049	Argininosuccinate lyase	PANTHER	PTHR43814	ARGININOSUCCINATE LYASE	coord: 29..353
IPR009049	Argininosuccinate lyase	CDD	cd01359	Argininosuccinate_lyase	coord: 52..351 e-value: 1.24844E-177 score: 498.61
IPR022761	Fumarate lyase, N-terminal	PFAM	PF00206	Lyase_1	coord: 36..330 e-value: 1.8E-70 score: 237.8
IPR024083	Fumarase/histidase, N-terminal	GENE3D	1.10.275.10		coord: 30..136 e-value: 2.3E-42 score: 145.6
IPR020557	Fumarate lyase, conserved site	PROSITE	PS00163	FUMARATE_LYASES	coord: 305..314
IPR008948	L-Aspartase-like	SUPERFAMILY	48557	L-aspartase-like	coord: 33..356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g015310.1	Lcy12g015310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042450	arginine biosynthetic process via ornithine
cellular_component	GO:0005829	cytosol
molecular_function	GO:0004056	argininosuccinate lyase activity
molecular_function	GO:0003824	catalytic activity