Lcy12g012080 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g012080
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionpolynucleotide 3'-phosphatase ZDP
LocationChr12: 39640670 .. 39644733 (-)
RNA-Seq ExpressionLcy12g012080
SyntenyLcy12g012080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCTCTTCTACGTTCTACTCACAACCTATGCTGATCGCTTCCACATTCCGTACGAGTCAAACCAGCTGCAGCTGCAAGCACATATTCTCTTCTCTCGCTCAAAATCCCACCTCCCCACTGAACCATTTCTCCTTATTTTCTCACAAACCCAATCATTTCAGCCGTATTTTCTTCCAATCCCCACCAATGTCACCGTCTTCTTTAAAAATCCTCGCCGAGTACGCCAAGTCGAATCGCTCTACGTGCAAGGGCTGCTCCAAAGCCATTCCGGCGAAGGCTCTGAGGTTGGGTTTCGTGAGCAGAGATGGGCGGGGATTCGACATGACCCGGTGGTTTCATTCCGATTGCGCTTCGTTTGGTTCAGACCCTGCTTGTTCCGCCGAGGAGATTAATGGGTTTGCACTCTTGAAGGCATGTTAGAATAGAAAGCTTTTGTAGTGTCTGTTTTCTTTTCTGTGAAATGGGGGAACGTTGACTGCTTTTAGTAATTTAGCTTAAAGGGGGAAGTTCAAATTTGTGTTCATTCAACGAAAGAGATGTTCTTTTTACTGGGTCTAGCATGTGTTCGATAAAATGCTTCTGAGATTCGAAGTGTGTTCATTTCATCTGCTTTGTTTTGTTTATTTTTCTTTTTCAGGGTAGTGATCAGGAAGCTCTGAAGAAATTGATCAGCAGAAGCCATGAAGCTAAGGTGACTGTTTTGCTCACGTTTCTGTGTCATGTTGCTTGATTTATTCATTATGTTCATCAGTTAGAATTTGGGTCTGGATTGATCTAATGTTAGATATCATTATTGGGTGTCATTTGAGTGATCATCATAAATTATTTGGTTAAATTTTCTCTTTGATTCAATGCTTTAATATGCCTATTCTTCTCATCTTGGTTACGTAATGCATCTCCAATAAACAGGCTTGCAAAAGGGATAGGGATGAAGAAGATGGAATTGAAGAAAGAGATCAAAAGAAAGTTGAGGTATGTATCTAAAGACTGATGCCCTTTGCAGAGCGTTTTCAGCATTTTTAAGTTATATCGTGCAATTGTATTTTTAATAGAGTATGTTCATGTCATCAATGGAACTGGTGTACCGAAACGGGCTCCTGCTCTCTTTGATTTTACATACAATGTCTTTAGTCCTTGGTGTACTGAACAATATGAAATGCTGCTATAAAATTTCAGTTTAAGCTGTAGCAACTGTCGAAACCATTGTTCTGAAATTTCATATCCATTTTAAGTCATACTTTCTCGATATCCATCTAAAACTAAAAGAAACAATTTGGTTTAGCTACTGAGATGGAAAATGGAATGGAATCCATTGAGTGAAGCCTCCTTATGTGCCATCATTTAATGCCTTATTTTCTAGATCCTGAATTGGTTTTCTTAACTAGTTAACTTTGTTCAAATCTTCATCCTATACGATTTCCTTGCTTATGCTGGCTGCAGCTCTTGCTTTCTTGTTTGATTTCTCTAGATATTTATTCTATGCTCTATCTTACATAGTATCTTCCATATTTTTTTGTACTTTCATCTTCTTTAACAAAGCTGCAGTTCTGTCCTACATGTTTTCTAGCATAAGAGTTAAGTTACAAATATAAAACCCTCTTACTATCAATTTCATTGCTTGCAGCTGTCTACCTCCAGTTTGCAGCCAAAGTTAGATCTCGTAATTACAGCCTCCGACATAAAGAACAAGTATAAGGTAGGATCCACTCATTGTTACTTTTCTTCATTCTCATTACTAGGTGGTTTTTGTCCTCTTTCCTTAGCTGAAATGTGTTTTGAATAGTTGACTAGATGAACTCACTTAAGCCTGCTAGTTTAGAGGCAATAAAAAGTTATAGAGATATATATCCATGCCCAAAAAACATACGCAGGACAGATTCTAGACTAATCCAGTCCCGTCATTTTCTTTTAAGCTGTGGCATCATAGATTATTATTTCGCTCTTATGTAATCCTAATCACAATTCACACGTAACTAAGTTTTTAATGATTCAGGACGCAACACTTTTACCTAAATGGAAGGCCTTCCAGACACTCATCTTTCTTGAGCAGGTAAAAAAAATATTTATAATTCCGTTTCTGAGCCATCATGTTGATGTTGAATTTTGATGGAGCGACATGGACAAATATGAATGAAAATTTAGTAGAATGTTTATTTCTTCTGCTGCTTCTGCTGAAACTCTATCTTTGAATTTAGAACTTGTTTTGGATAATGTTAGTTGATATTGCTTACTCTTAAACACGCAATCCAGCGATTAGGTGTACACAGACTTCACCTTTTAGATTACTCATACAAATTGATATATTTGTACTCTTTACTATGTTAATGTGATTCACTTTTTCTATTTCTTTGCAGGATGATGGGCTACATCATTCAAGTAAAATAGCTGCATTTGATTTCGATGGCTGTCTGGCAAACACATCTGTTAAAAGGTATGGCTGTACCCGAAGTGGAAGTTTGGATTTTGTCGTTGCGCGTGTGTTAAAACTTAAAGGTCTTTAGATTGATAGGTATTTTATTAGGTGTTTTTGGCTACACTTTATCCTTGAGATAGACTAGCTTCACTATATGCATACATTCTCTTGCTAAGTTTTCTCCACTTATGTTCACATTAATCTCTCTCTCCACACATATTTTCTCTCACTAACTTCTCTCAATTCATAGTTTCCCCCCCTAAGTTTTCTCCATACAGTTTTTGATTTAGTCTCTCTCTCCACAAATTCTCTCATTAACTCCTCATTACACACATTTCCTCTCACTAATTTCTCTTTAAACATATATTTCCTCTCACAAATTTCTATCTACACACTTTCTTTCTTTAGCTTCTCTCCATACATCTCGATCTCTTTCTTCACTCATATGTGTCTTGAATCCCTTTCTCTTGTTCTGGAGAATAATTGAGTTCTAGTAATTTTAATTGTACAAAGCACTTCCCTTCTTTCTTTGTTTAGACGTGGGGTGTTCTAATTTCAGAGTAGGTGCAGAGGCGTGGTCTCTGATGTACCCTTCAATACCACAAAAACTTCAGAGCTTGTACAATGATGGCTACAAGCTGGTTTGTCCCCCTTCTCCATGAATTATACTCAACTATTCTCTCTCTTTGTTATGAAATTATTATTGATTAACATTACTGTGAGCAGGTAATATTCACGAACGAATCCAACATTGACAGATGGAAGAACAAGAGACAAGTTGCTGTGGACTCCAAACTTGGGCGTCTCAATAATTTTATCAGTCAAGTCAATGTCCCAATTCAGGTAACTACCAGATCAGAGTCACCAGACACATTGCAATTGCATCCATTCACTGCAACCTTGTTGAAGTGTGAGTGTATGATGTGTTGTTGTGTGGAATGTGGATGCAGGTGTTTGTTGCATGTGGAATTGGAAGCGGGTCGGGTAAATCCAAAGAGGACCCATTTAGGAAACCGAAACCCGGGATGTGGCACATCATGGAGAACCACTTCAACGACGGCATTCCCATTGATCTCGACCAGTTATTTCTCTCTCTCTCAACTTGAAAAGTGTCAAATAAGTCATTCAACTTTTAATTTTATGTTCAACATATCTTAATGAGTCCCACATCAATAGGAACTTGCTAGACACAAAATTTGTAAAATTTGTAAGTTTACAAATTTGAGAAATTCATGGACACAACATTATAGTCTAGGGACTTAATAGACATTATAAAGTCTAGACCAAACTTATAAAGTTAATGCCTGCCCTGGTTGATAGATGCTTTTACGTGGGGGATGCAGCTGGGAGAGCCAAGGATCACAGTGATGCTGATATCAAGTTTGCTCAGGTACTACTAATTATTCTATTCTCATACCATTACTAACATATACCACTTCTTTTTTCTTCTTTATATTTTGTTTTCTTGCAATTTTGCAGGCCATTGGGTTGAAGTTTTATGTTCCTGAAGAATTTTTTGTAACTTCCCCTTCACCAGCCGATGACGATGTGTAGCCATAGCATTGTAATTAATCACTCGTTCTCAAGTTTGGATGTAGTTGATTCAGTGGAGGATTTTAAATGATGGCCCATTTTCTCGAACTAAATTTA

mRNA sequence

GTTCTCTTCTACGTTCTACTCACAACCTATGCTGATCGCTTCCACATTCCGTACGAGTCAAACCAGCTGCAGCTGCAAGCACATATTCTCTTCTCTCGCTCAAAATCCCACCTCCCCACTGAACCATTTCTCCTTATTTTCTCACAAACCCAATCATTTCAGCCGTATTTTCTTCCAATCCCCACCAATGTCACCGTCTTCTTTAAAAATCCTCGCCGAGTACGCCAAGTCGAATCGCTCTACGTGCAAGGGCTGCTCCAAAGCCATTCCGGCGAAGGCTCTGAGGTTGGGTTTCGTGAGCAGAGATGGGCGGGGATTCGACATGACCCGGTGGTTTCATTCCGATTGCGCTTCGTTTGGTTCAGACCCTGCTTGTTCCGCCGAGGAGATTAATGGGTTTGCACTCTTGAAGGGTAGTGATCAGGAAGCTCTGAAGAAATTGATCAGCAGAAGCCATGAAGCTAAGGCTTGCAAAAGGGATAGGGATGAAGAAGATGGAATTGAAGAAAGAGATCAAAAGAAAGTTGAGCTGTCTACCTCCAGTTTGCAGCCAAAGTTAGATCTCGTAATTACAGCCTCCGACATAAAGAACAAGTATAAGGACGCAACACTTTTACCTAAATGGAAGGCCTTCCAGACACTCATCTTTCTTGAGCAGGATGATGGGCTACATCATTCAAGTAAAATAGCTGCATTTGATTTCGATGGCTGTCTGGCAAACACATCTGTTAAAAGAGTAGGTGCAGAGGCGTGGTCTCTGATGTACCCTTCAATACCACAAAAACTTCAGAGCTTGTACAATGATGGCTACAAGCTGGTAATATTCACGAACGAATCCAACATTGACAGATGGAAGAACAAGAGACAAGTTGCTGTGGACTCCAAACTTGGGCGTCTCAATAATTTTATCAGTCAAGTCAATGTCCCAATTCAGGTGTTTGTTGCATGTGGAATTGGAAGCGGGTCGGGTAAATCCAAAGAGGACCCATTTAGGAAACCGAAACCCGGGATGTGGCACATCATGGAGAACCACTTCAACGACGGCATTCCCATTGATCTCGACCAATGCTTTTACGTGGGGGATGCAGCTGGGAGAGCCAAGGATCACAGTGATGCTGATATCAAGTTTGCTCAGGCCATTGGGTTGAAGTTTTATGTTCCTGAAGAATTTTTTGTAACTTCCCCTTCACCAGCCGATGACGATGTGTAGCCATAGCATTGTAATTAATCACTCGTTCTCAAGTTTGGATGTAGTTGATTCAGTGGAGGATTTTAAATGATGGCCCATTTTCTCGAACTAAATTTA

Coding sequence (CDS)

ATGCTGATCGCTTCCACATTCCGTACGAGTCAAACCAGCTGCAGCTGCAAGCACATATTCTCTTCTCTCGCTCAAAATCCCACCTCCCCACTGAACCATTTCTCCTTATTTTCTCACAAACCCAATCATTTCAGCCGTATTTTCTTCCAATCCCCACCAATGTCACCGTCTTCTTTAAAAATCCTCGCCGAGTACGCCAAGTCGAATCGCTCTACGTGCAAGGGCTGCTCCAAAGCCATTCCGGCGAAGGCTCTGAGGTTGGGTTTCGTGAGCAGAGATGGGCGGGGATTCGACATGACCCGGTGGTTTCATTCCGATTGCGCTTCGTTTGGTTCAGACCCTGCTTGTTCCGCCGAGGAGATTAATGGGTTTGCACTCTTGAAGGGTAGTGATCAGGAAGCTCTGAAGAAATTGATCAGCAGAAGCCATGAAGCTAAGGCTTGCAAAAGGGATAGGGATGAAGAAGATGGAATTGAAGAAAGAGATCAAAAGAAAGTTGAGCTGTCTACCTCCAGTTTGCAGCCAAAGTTAGATCTCGTAATTACAGCCTCCGACATAAAGAACAAGTATAAGGACGCAACACTTTTACCTAAATGGAAGGCCTTCCAGACACTCATCTTTCTTGAGCAGGATGATGGGCTACATCATTCAAGTAAAATAGCTGCATTTGATTTCGATGGCTGTCTGGCAAACACATCTGTTAAAAGAGTAGGTGCAGAGGCGTGGTCTCTGATGTACCCTTCAATACCACAAAAACTTCAGAGCTTGTACAATGATGGCTACAAGCTGGTAATATTCACGAACGAATCCAACATTGACAGATGGAAGAACAAGAGACAAGTTGCTGTGGACTCCAAACTTGGGCGTCTCAATAATTTTATCAGTCAAGTCAATGTCCCAATTCAGGTGTTTGTTGCATGTGGAATTGGAAGCGGGTCGGGTAAATCCAAAGAGGACCCATTTAGGAAACCGAAACCCGGGATGTGGCACATCATGGAGAACCACTTCAACGACGGCATTCCCATTGATCTCGACCAATGCTTTTACGTGGGGGATGCAGCTGGGAGAGCCAAGGATCACAGTGATGCTGATATCAAGTTTGCTCAGGCCATTGGGTTGAAGTTTTATGTTCCTGAAGAATTTTTTGTAACTTCCCCTTCACCAGCCGATGACGATGTGTAG

Protein sequence

MLIASTFRTSQTSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKILAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEINGFALLKGSDQEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQPKLDLVITASDIKNKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAIGLKFYVPEEFFVTSPSPADDDV
Homology
BLAST of Lcy12g012080 vs. ExPASy Swiss-Prot
Match: Q84JE8 (Polynucleotide 3'-phosphatase ZDP OS=Arabidopsis thaliana OX=3702 GN=ZDP PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 7.3e-116
Identity = 211/367 (57.49%), Postives = 264/367 (71.93%), Query Frame = 0

Query: 55  SPSSLKILAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP 114
           SP S K+++EYAKS+RSTCK CS+ I AK LRLG V+R+ RGFDM +W H  C    SDP
Sbjct: 322 SPDSSKVISEYAKSSRSTCKKCSQTIAAKELRLGLVTRNFRGFDMKQWHHLGCFPVDSDP 381

Query: 115 ACSAEEINGFALLKGSDQEALKKLISR-SHEAKACKRDRDEEDG------IEERDQKK-- 174
             S E+I GF+ L+  DQ+ALK+L+ +   +    K D D +D        EE +++K  
Sbjct: 382 IVSVEDIGGFSELQSGDQDALKELVQQCGKQTLVDKMDEDNDDTEAKIKLTEETNKRKHS 441

Query: 175 --------------------------VELSTSSLQPKLDLVITASDIKNKYKDATLLPKW 234
                                     +  STS ++ + ++ ++ASD+K+KY+DA LLPKW
Sbjct: 442 EVGEMVEEDESLTKAKQQMAKTHKVNMSESTSQVEVEAEITLSASDVKDKYRDANLLPKW 501

Query: 235 KAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQKLQSLYND 294
           KAF+T+IFLE+DDGL+ S KIAAFDFDGCLA TSVK VGA+AWSLMYPSIP+KLQSL++ 
Sbjct: 502 KAFETVIFLERDDGLNDSEKIAAFDFDGCLAKTSVKIVGADAWSLMYPSIPEKLQSLHDQ 561

Query: 295 GYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGSGK-SKE 354
           GYKLVIFTNESNIDRWKNKRQ AVDSK+GRLN+FI +V VPIQVF+ACG+ S  GK  K+
Sbjct: 562 GYKLVIFTNESNIDRWKNKRQAAVDSKIGRLNSFIERVKVPIQVFIACGVSSSGGKGGKD 621

Query: 355 DPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAIGLKFYVP 386
           D +RKPK GMW +M+ HFN GI ID+D+ FYVGDAAGR  DHSDADIKFAQA GLKF+ P
Sbjct: 622 DLYRKPKAGMWQLMKKHFNSGIAIDMDKSFYVGDAAGRKMDHSDADIKFAQASGLKFFTP 681

BLAST of Lcy12g012080 vs. ExPASy Swiss-Prot
Match: Q9JLV6 (Bifunctional polynucleotide phosphatase/kinase OS=Mus musculus OX=10090 GN=Pnkp PE=1 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-28
Identity = 77/201 (38.31%), Postives = 104/201 (51.74%), Query Frame = 0

Query: 199 WKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRV---GAEAWSLMYPSIPQKLQS 258
           W++ + L+      G+    K+AAFD DG L  T   +V       W ++YP IP+KLQ 
Sbjct: 146 WESLKKLLVFTA-SGVKPQGKVAAFDLDGTLITTRSGKVFPTSPSDWRILYPEIPKKLQE 205

Query: 259 LYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGSGK 318
           L  +GYKLVIFTN+  I R K   +V      G++   + ++ VP QV VA   G     
Sbjct: 206 LAAEGYKLVIFTNQMGIGRGKLPAEVF----KGKVEAVLEKLGVPFQVLVATHAGLN--- 265

Query: 319 SKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGR---------AKDHSDADIK 378
                 RKP  GMW  ++   N+GIPI ++   +VGDAAGR          KD S AD  
Sbjct: 266 ------RKPVSGMWDHLQEQANEGIPISVEDSVFVGDAAGRLANWAPGRKKKDFSCADRL 325

Query: 379 FAQAIGLKFYVPEEFFVTSPS 388
           FA  +GL F  PEEFF+  P+
Sbjct: 326 FALNVGLPFATPEEFFLKWPA 332

BLAST of Lcy12g012080 vs. ExPASy Swiss-Prot
Match: Q96T60 (Bifunctional polynucleotide phosphatase/kinase OS=Homo sapiens OX=9606 GN=PNKP PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 5.7e-28
Identity = 75/203 (36.95%), Postives = 103/203 (50.74%), Query Frame = 0

Query: 197 PKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRV---GAEAWSLMYPSIPQKL 256
           P W+  + L+      G+    K+A FD DG L  T   +V   G   W ++YP IP+KL
Sbjct: 145 PGWENLEKLLVFTA-AGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKL 204

Query: 257 QSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGS 316
           + L  +GYKLVIFTN+ +I R K    +  +    ++   + ++ VP QV VA   G   
Sbjct: 205 RELEAEGYKLVIFTNQMSIGRGK----LPAEEFKAKVEAVVEKLGVPFQVLVATHAGL-- 264

Query: 317 GKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGR---------AKDHSDAD 376
                  +RKP  GMW  ++   NDG PI +    +VGDAAGR          KD S AD
Sbjct: 265 -------YRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCAD 324

Query: 377 IKFAQAIGLKFYVPEEFFVTSPS 388
             FA  +GL F  PEEFF+  P+
Sbjct: 325 RLFALNLGLPFATPEEFFLKWPA 333

BLAST of Lcy12g012080 vs. ExPASy Swiss-Prot
Match: O13911 (Bifunctional polynucleotide phosphatase/kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pnk1 PE=1 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 8.6e-24
Identity = 66/181 (36.46%), Postives = 96/181 (53.04%), Query Frame = 0

Query: 206 IFLEQDDGLHHSSKIAAFDFDGCLANTSVKRV---GAEAWSLMYPSIPQKLQSLYNDGYK 265
           +++ +   L  + K  AFD DG L  T   RV    A  W+  +PS+  KL++LY D Y 
Sbjct: 56  LYIAKYGKLKKTKKFIAFDLDGTLIKTKSGRVFSKDAADWTWWHPSVVPKLKALYQDNYS 115

Query: 266 LVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGSGKSKEDPFR 325
           LVIF+N++ I R    +  A  +   ++      +++PI ++ A           +D FR
Sbjct: 116 LVIFSNQNGIPR----KPSAGHTFQMKIRAIFESLDLPIVLYAAI---------LKDKFR 175

Query: 326 KPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAIGLKFYVPEEFF 384
           KP  GMW+      N    IDL    YVGDAAGR  DH+  D+KFA+ IG+KF  PE+FF
Sbjct: 176 KPLTGMWNSFLKDVNRS--IDLSFIKYVGDAAGRPGDHNSTDLKFAENIGIKFETPEQFF 221

BLAST of Lcy12g012080 vs. ExPASy Swiss-Prot
Match: Q19683 (Uncharacterized protein F21D5.5 OS=Caenorhabditis elegans OX=6239 GN=F21D5.5 PE=2 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.9e-23
Identity = 66/174 (37.93%), Postives = 94/174 (54.02%), Query Frame = 0

Query: 219 KIAAFDFDGCLANTSVKRV---GAEAWSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRW 278
           KIAAFD DG L  T   +V     + W L+Y SIP   + L++DG+K+VIFTN+  I   
Sbjct: 52  KIAAFDMDGTLIKTKSGKVFPTNCQDWQLLYDSIPSDFKKLHSDGFKIVIFTNQKGIHAG 111

Query: 279 KNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENH 338
           K  R    +    ++   + ++ +P+Q FV+   G          +RKP  GMW+ ++  
Sbjct: 112 KVDR----NEFRKKIEAIVGKLGIPVQAFVSVAGGH---------YRKPCVGMWNELKLR 171

Query: 339 FNDGIPIDLDQCFYVGDAAGR-------AKDHSDADIKFAQAIGLKFYVPEEFF 383
            ND + I+  +  +VGDAAGR        KDHS AD  FA  +G+KF  PEEFF
Sbjct: 172 -NDEVEINEKESIFVGDAAGRIKTTSRPKKDHSYADRFFAANVGVKFQTPEEFF 211

BLAST of Lcy12g012080 vs. ExPASy TrEMBL
Match: A0A6J1F9Q9 (polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443571 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.7e-182
Identity = 320/372 (86.02%), Postives = 340/372 (91.40%), Query Frame = 0

Query: 12  TSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKILAEYAKSNRS 71
           T C+  HI SSL Q P SPL+HFS FS   +H  RIFFQ PPMS S++KI+AEYAKSNRS
Sbjct: 2   TYCN-NHILSSLLQIPKSPLSHFSRFS---SHSRRIFFQGPPMSTSAVKIVAEYAKSNRS 61

Query: 72  TCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEINGFALLKGSD 131
           TCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP  SAEEINGFALLKG D
Sbjct: 62  TCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPVSSAEEINGFALLKGDD 121

Query: 132 QEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQPKLDLVITASDIKNKYK 191
           QEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS SSL PKLDLV+TAS+IK KYK
Sbjct: 122 QEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSASSLLPKLDLVLTASNIKTKYK 181

Query: 192 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ 251
           DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYP IP 
Sbjct: 182 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPLIPH 241

Query: 252 KLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGS 311
           KLQSLYN+GYKLVIFTNESNI+RWKNKRQVAVDSKLGRL++FIS+VNVP+QVFVACGIG 
Sbjct: 242 KLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRVNVPVQVFVACGIGG 301

Query: 312 GSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAI 371
           GSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVGDAAGRAKDHSD D++FAQAI
Sbjct: 302 GSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRAKDHSDTDLRFAQAI 361

Query: 372 GLKFYVPEEFFV 384
           GLKFYVPEEFFV
Sbjct: 362 GLKFYVPEEFFV 369

BLAST of Lcy12g012080 vs. ExPASy TrEMBL
Match: A0A6J1IH48 (polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476896 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.8e-181
Identity = 319/372 (85.75%), Postives = 341/372 (91.67%), Query Frame = 0

Query: 12  TSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKILAEYAKSNRS 71
           T C+  HI SSL Q P SPL+HFS FS   +H  RIFFQ P MS S++KI+AEYAKSNRS
Sbjct: 2   TYCN-NHILSSLLQIPNSPLSHFSRFS---SHSRRIFFQGPTMSTSAVKIVAEYAKSNRS 61

Query: 72  TCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEINGFALLKGSD 131
           TCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP  SAE+INGFALLKG D
Sbjct: 62  TCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPVSSAEQINGFALLKGDD 121

Query: 132 QEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQPKLDLVITASDIKNKYK 191
           QEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS SSL PKLDLV++AS+IK KYK
Sbjct: 122 QEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSASSLLPKLDLVLSASNIKTKYK 181

Query: 192 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ 251
           DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ
Sbjct: 182 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ 241

Query: 252 KLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGS 311
           KLQSLYN+GYKLVIFTNESNI+RWKNKRQVAVDSKLGRL++FIS+VNVP+QVFVACGIGS
Sbjct: 242 KLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRVNVPVQVFVACGIGS 301

Query: 312 GSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAI 371
           GSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVGDAAGR KDHSDAD++FAQAI
Sbjct: 302 GSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRGKDHSDADLRFAQAI 361

Query: 372 GLKFYVPEEFFV 384
           GLKFYVPEE FV
Sbjct: 362 GLKFYVPEEIFV 369

BLAST of Lcy12g012080 vs. ExPASy TrEMBL
Match: A0A6J1FAH6 (polynucleotide 3'-phosphatase ZDP isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443571 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.4e-172
Identity = 297/332 (89.46%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 52  PPMSPSSLKILAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFG 111
           PPMS S++KI+AEYAKSNRSTCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFG
Sbjct: 5   PPMSTSAVKIVAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFG 64

Query: 112 SDPACSAEEINGFALLKGSDQEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTS 171
           SDP  SAEEINGFALLKG DQEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS S
Sbjct: 65  SDPVSSAEEINGFALLKGDDQEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSAS 124

Query: 172 SLQPKLDLVITASDIKNKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN 231
           SL PKLDLV+TAS+IK KYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN
Sbjct: 125 SLLPKLDLVLTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN 184

Query: 232 TSVKRVGAEAWSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLN 291
           TSVKRVGAEAWSLMYP IP KLQSLYN+GYKLVIFTNESNI+RWKNKRQVAVDSKLGRL+
Sbjct: 185 TSVKRVGAEAWSLMYPLIPHKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLD 244

Query: 292 NFISQVNVPIQVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVG 351
           +FIS+VNVP+QVFVACGIG GSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVG
Sbjct: 245 SFISRVNVPVQVFVACGIGGGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVG 304

Query: 352 DAAGRAKDHSDADIKFAQAIGLKFYVPEEFFV 384
           DAAGRAKDHSD D++FAQAIGLKFYVPEEFFV
Sbjct: 305 DAAGRAKDHSDTDLRFAQAIGLKFYVPEEFFV 336

BLAST of Lcy12g012080 vs. ExPASy TrEMBL
Match: A0A1S3CLI9 (polynucleotide 3'-phosphatase ZDP OS=Cucumis melo OX=3656 GN=LOC103501815 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.7e-171
Identity = 309/392 (78.83%), Postives = 333/392 (84.95%), Query Frame = 0

Query: 13  SCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKILAEYAKSNRST 72
           +CS   + SSL + PTSP  +   FS   +HF+  F Q PPMS SSLKI+AEYAKSNRST
Sbjct: 2   TCSNLILLSSLPKFPTSPFIYSLSFS---SHFTPFFLQRPPMSSSSLKIVAEYAKSNRST 61

Query: 73  CKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEINGFALLKGSDQ 132
           CK CSK IPA  LRLGFV+RDGRGFDMTRWFHS CASFGS P  SAE+INGFALLK SDQ
Sbjct: 62  CKNCSKTIPASTLRLGFVTRDGRGFDMTRWFHSHCASFGSHPVSSAEDINGFALLKDSDQ 121

Query: 133 EALKKLISRSHEAKACKRDRDEEDGIEERDQKK---------------------VELSTS 192
           EALKK++ RSHE+K  KRD DE+DG+EERDQKK                     V+LSTS
Sbjct: 122 EALKKMVCRSHESKVHKRDGDEKDGVEERDQKKVKVHQKDGDEEYGVEERDQKNVKLSTS 181

Query: 193 SLQPKLDLVITASDIKNKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN 252
           SLQPKLDLV+TASDIK KYKDATLL KWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN
Sbjct: 182 SLQPKLDLVLTASDIKTKYKDATLLAKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN 241

Query: 253 TSVKRVGAEAWSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLN 312
           TSVKRVGAEAWSLMYPSIPQKLQSL++DGYKLVIFTNESNI+RWKNKRQVAVDSKLGRLN
Sbjct: 242 TSVKRVGAEAWSLMYPSIPQKLQSLHDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLN 301

Query: 313 NFISQVNVPIQVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVG 372
           NFISQV VP+QVFVACGIGS SGKS+ED FRKPKPGMWHIME+HFN G+PID+DQCFYVG
Sbjct: 302 NFISQVKVPVQVFVACGIGSKSGKSEEDTFRKPKPGMWHIMESHFNGGVPIDIDQCFYVG 361

Query: 373 DAAGRAKDHSDADIKFAQAIGLKFYVPEEFFV 384
           DAAGRAKDHSDADIKFAQAIGLKFYVPEEFFV
Sbjct: 362 DAAGRAKDHSDADIKFAQAIGLKFYVPEEFFV 390

BLAST of Lcy12g012080 vs. ExPASy TrEMBL
Match: A0A6J1IFZ5 (polynucleotide 3'-phosphatase ZDP isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476896 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.2e-171
Identity = 296/332 (89.16%), Postives = 315/332 (94.88%), Query Frame = 0

Query: 52  PPMSPSSLKILAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFG 111
           P MS S++KI+AEYAKSNRSTCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFG
Sbjct: 5   PTMSTSAVKIVAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFG 64

Query: 112 SDPACSAEEINGFALLKGSDQEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTS 171
           SDP  SAE+INGFALLKG DQEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS S
Sbjct: 65  SDPVSSAEQINGFALLKGDDQEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSAS 124

Query: 172 SLQPKLDLVITASDIKNKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN 231
           SL PKLDLV++AS+IK KYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN
Sbjct: 125 SLLPKLDLVLSASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLAN 184

Query: 232 TSVKRVGAEAWSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLN 291
           TSVKRVGAEAWSLMYPSIPQKLQSLYN+GYKLVIFTNESNI+RWKNKRQVAVDSKLGRL+
Sbjct: 185 TSVKRVGAEAWSLMYPSIPQKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLD 244

Query: 292 NFISQVNVPIQVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVG 351
           +FIS+VNVP+QVFVACGIGSGSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVG
Sbjct: 245 SFISRVNVPVQVFVACGIGSGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVG 304

Query: 352 DAAGRAKDHSDADIKFAQAIGLKFYVPEEFFV 384
           DAAGR KDHSDAD++FAQAIGLKFYVPEE FV
Sbjct: 305 DAAGRGKDHSDADLRFAQAIGLKFYVPEEIFV 336

BLAST of Lcy12g012080 vs. NCBI nr
Match: XP_038898753.1 (polynucleotide 3'-phosphatase ZDP [Benincasa hispida])

HSP 1 Score: 656.0 bits (1691), Expect = 2.1e-184
Identity = 326/385 (84.68%), Postives = 349/385 (90.65%), Query Frame = 0

Query: 1   MLIAS-TFRTSQTSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSL 60
           MLI + TFRT+  +C CKHI SSL Q  TSP NHFS FS   +HF+  FFQ PPMS SS 
Sbjct: 1   MLIGTFTFRTNHMTC-CKHILSSLTQISTSPFNHFSSFS---SHFTLFFFQHPPMSSSSP 60

Query: 61  KILAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMT-RWFHSDCASFGSDPACSA 120
           KI+AEYAKSNRSTCK CSK IPA  LRLGFV RDGRGFDMT RWFHSDCASFGSDP  SA
Sbjct: 61  KIVAEYAKSNRSTCKNCSKTIPANTLRLGFVIRDGRGFDMTPRWFHSDCASFGSDPVSSA 120

Query: 121 EEINGFALLKGSDQEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQPKLD 180
           E+INGFALLK  DQEALKKL+ RSHE+K  K DRDEEDG+EERDQKKV+LSTSSLQPKL+
Sbjct: 121 EDINGFALLKDIDQEALKKLVCRSHESKVYKTDRDEEDGVEERDQKKVKLSTSSLQPKLN 180

Query: 181 LVITASDIKNKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVG 240
           LV+TAS+IK KYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVG
Sbjct: 181 LVLTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVG 240

Query: 241 AEAWSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVN 300
           AEAWSLMYPSIPQKL+SLYNDGYKLVIFTNESNI+RWKNKRQVAVDSKLGRLNNFISQVN
Sbjct: 241 AEAWSLMYPSIPQKLRSLYNDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQVN 300

Query: 301 VPIQVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAK 360
           VP+QVFVACGIGSGSGKS+EDPFRKPKPGMWH+ME HFNDGIP+++DQCFYVGDAAGRAK
Sbjct: 301 VPVQVFVACGIGSGSGKSEEDPFRKPKPGMWHVMEEHFNDGIPVNIDQCFYVGDAAGRAK 360

Query: 361 DHSDADIKFAQAIGLKFYVPEEFFV 384
           DHSD D+KFAQAIGLKFYVPEEFFV
Sbjct: 361 DHSDTDLKFAQAIGLKFYVPEEFFV 381

BLAST of Lcy12g012080 vs. NCBI nr
Match: XP_023535258.1 (polynucleotide 3'-phosphatase ZDP isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.2 bits (1676), Expect = 1.1e-182
Identity = 322/382 (84.29%), Postives = 345/382 (90.31%), Query Frame = 0

Query: 2   LIASTFRTSQTSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKI 61
           LI ST RTS  +    HI SSL Q P SPL+HFS FS   +H  RIFFQ   MS S++KI
Sbjct: 67  LIGSTVRTSHMTYCNNHILSSLLQIPNSPLSHFSRFS---SHSRRIFFQGSSMSTSAVKI 126

Query: 62  LAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEI 121
           +AEYAKSNRSTCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP  SA++I
Sbjct: 127 VAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPVSSADKI 186

Query: 122 NGFALLKGSDQEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQPKLDLVI 181
           NGFALLKG DQEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS SSL PKLDLV+
Sbjct: 187 NGFALLKGDDQEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSASSLLPKLDLVL 246

Query: 182 TASDIKNKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEA 241
           TAS+IK KYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEA
Sbjct: 247 TASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEA 306

Query: 242 WSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPI 301
           WSLMYPSIPQKLQSLYN+GYKLV+FTNESNI+RWKNKRQVAVDSKLGRL++FIS+VNVP+
Sbjct: 307 WSLMYPSIPQKLQSLYNEGYKLVVFTNESNIERWKNKRQVAVDSKLGRLDSFISRVNVPV 366

Query: 302 QVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHS 361
           QVFVACGIG GSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVGDAAGRAKDHS
Sbjct: 367 QVFVACGIGGGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRAKDHS 426

Query: 362 DADIKFAQAIGLKFYVPEEFFV 384
           DAD++FAQ IGLKFYVPEEFFV
Sbjct: 427 DADLRFAQVIGLKFYVPEEFFV 445

BLAST of Lcy12g012080 vs. NCBI nr
Match: XP_022937206.1 (polynucleotide 3'-phosphatase ZDP isoform X1 [Cucurbita moschata])

HSP 1 Score: 647.1 bits (1668), Expect = 9.6e-182
Identity = 320/372 (86.02%), Postives = 340/372 (91.40%), Query Frame = 0

Query: 12  TSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKILAEYAKSNRS 71
           T C+  HI SSL Q P SPL+HFS FS   +H  RIFFQ PPMS S++KI+AEYAKSNRS
Sbjct: 2   TYCN-NHILSSLLQIPKSPLSHFSRFS---SHSRRIFFQGPPMSTSAVKIVAEYAKSNRS 61

Query: 72  TCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEINGFALLKGSD 131
           TCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP  SAEEINGFALLKG D
Sbjct: 62  TCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPVSSAEEINGFALLKGDD 121

Query: 132 QEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQPKLDLVITASDIKNKYK 191
           QEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS SSL PKLDLV+TAS+IK KYK
Sbjct: 122 QEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSASSLLPKLDLVLTASNIKTKYK 181

Query: 192 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ 251
           DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYP IP 
Sbjct: 182 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPLIPH 241

Query: 252 KLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGS 311
           KLQSLYN+GYKLVIFTNESNI+RWKNKRQVAVDSKLGRL++FIS+VNVP+QVFVACGIG 
Sbjct: 242 KLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRVNVPVQVFVACGIGG 301

Query: 312 GSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAI 371
           GSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVGDAAGRAKDHSD D++FAQAI
Sbjct: 302 GSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRAKDHSDTDLRFAQAI 361

Query: 372 GLKFYVPEEFFV 384
           GLKFYVPEEFFV
Sbjct: 362 GLKFYVPEEFFV 369

BLAST of Lcy12g012080 vs. NCBI nr
Match: XP_022976526.1 (polynucleotide 3'-phosphatase ZDP isoform X1 [Cucurbita maxima])

HSP 1 Score: 645.2 bits (1663), Expect = 3.7e-181
Identity = 319/372 (85.75%), Postives = 341/372 (91.67%), Query Frame = 0

Query: 12  TSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKILAEYAKSNRS 71
           T C+  HI SSL Q P SPL+HFS FS   +H  RIFFQ P MS S++KI+AEYAKSNRS
Sbjct: 2   TYCN-NHILSSLLQIPNSPLSHFSRFS---SHSRRIFFQGPTMSTSAVKIVAEYAKSNRS 61

Query: 72  TCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEINGFALLKGSD 131
           TCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP  SAE+INGFALLKG D
Sbjct: 62  TCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPVSSAEQINGFALLKGDD 121

Query: 132 QEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQPKLDLVITASDIKNKYK 191
           QEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS SSL PKLDLV++AS+IK KYK
Sbjct: 122 QEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSASSLLPKLDLVLSASNIKTKYK 181

Query: 192 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ 251
           DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ
Sbjct: 182 DATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQ 241

Query: 252 KLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGS 311
           KLQSLYN+GYKLVIFTNESNI+RWKNKRQVAVDSKLGRL++FIS+VNVP+QVFVACGIGS
Sbjct: 242 KLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRVNVPVQVFVACGIGS 301

Query: 312 GSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAI 371
           GSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVGDAAGR KDHSDAD++FAQAI
Sbjct: 302 GSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRGKDHSDADLRFAQAI 361

Query: 372 GLKFYVPEEFFV 384
           GLKFYVPEE FV
Sbjct: 362 GLKFYVPEEIFV 369

BLAST of Lcy12g012080 vs. NCBI nr
Match: KAG7024794.1 (Polynucleotide 3'-phosphatase ZDP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 634.4 bits (1635), Expect = 6.5e-178
Identity = 322/389 (82.78%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 12  TSCSCKHIFSSLAQNPTSPLNHFSLFSHKPNHFSRIFFQSPPMSPSSLKILAEYAKSNRS 71
           T C+  HI SSL Q P SPL+HFS FS   +H  RIFFQ PPMS S++KI+AEYAKSNRS
Sbjct: 2   TYCN-NHILSSLLQIPKSPLSHFSRFS---SHSRRIFFQGPPMSTSAVKIVAEYAKSNRS 61

Query: 72  TCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPACSAEEING-------- 131
           TCK CSK IPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP  SAEEING        
Sbjct: 62  TCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDPVSSAEEINGVHYLFDKM 121

Query: 132 --------FALLK-GSDQEALKKLISRSHEAKACKRDRDEEDGIEERDQKKVELSTSSLQ 191
                   F LL  G DQEALKKL+SRSHE+K  KRD DEEDGIEERDQKKVELS SSL 
Sbjct: 122 LLRFKLCSFHLLGFGDDQEALKKLVSRSHESKVRKRDEDEEDGIEERDQKKVELSASSLL 181

Query: 192 PKLDLVITASDIKNKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSV 251
           PKLDLV+TAS+IK KYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSV
Sbjct: 182 PKLDLVLTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSV 241

Query: 252 KRVGAEAWSLMYPSIPQKLQSLYNDGYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFI 311
           KRVGAEAWSLMYPSIPQKLQSLYN+GYKLVIFTNESNI+RWKNKRQVAVDSKLGRL++FI
Sbjct: 242 KRVGAEAWSLMYPSIPQKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFI 301

Query: 312 SQVNVPIQVFVACGIGSGSGKSKEDPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAA 371
           S+VNVP+QVFVACGIGSGSGKS+EDPFRKPKPGMW IMENHFN GIPI+LDQCFYVGDAA
Sbjct: 302 SRVNVPVQVFVACGIGSGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAA 361

Query: 372 GRAKDHSDADIKFAQAIGLKFYVPEEFFV 384
           GRAKDHSDAD++FAQAIGLKFYVPEEFFV
Sbjct: 362 GRAKDHSDADLRFAQAIGLKFYVPEEFFV 386

BLAST of Lcy12g012080 vs. TAIR 10
Match: AT3G14890.1 (phosphoesterase )

HSP 1 Score: 418.7 bits (1075), Expect = 5.2e-117
Identity = 211/367 (57.49%), Postives = 264/367 (71.93%), Query Frame = 0

Query: 55  SPSSLKILAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP 114
           SP S K+++EYAKS+RSTCK CS+ I AK LRLG V+R+ RGFDM +W H  C    SDP
Sbjct: 322 SPDSSKVISEYAKSSRSTCKKCSQTIAAKELRLGLVTRNFRGFDMKQWHHLGCFPVDSDP 381

Query: 115 ACSAEEINGFALLKGSDQEALKKLISR-SHEAKACKRDRDEEDG------IEERDQKK-- 174
             S E+I GF+ L+  DQ+ALK+L+ +   +    K D D +D        EE +++K  
Sbjct: 382 IVSVEDIGGFSELQSGDQDALKELVQQCGKQTLVDKMDEDNDDTEAKIKLTEETNKRKHS 441

Query: 175 --------------------------VELSTSSLQPKLDLVITASDIKNKYKDATLLPKW 234
                                     +  STS ++ + ++ ++ASD+K+KY+DA LLPKW
Sbjct: 442 EVGEMVEEDESLTKAKQQMAKTHKVNMSESTSQVEVEAEITLSASDVKDKYRDANLLPKW 501

Query: 235 KAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQKLQSLYND 294
           KAF+T+IFLE+DDGL+ S KIAAFDFDGCLA TSVK VGA+AWSLMYPSIP+KLQSL++ 
Sbjct: 502 KAFETVIFLERDDGLNDSEKIAAFDFDGCLAKTSVKIVGADAWSLMYPSIPEKLQSLHDQ 561

Query: 295 GYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGSGK-SKE 354
           GYKLVIFTNESNIDRWKNKRQ AVDSK+GRLN+FI +V VPIQVF+ACG+ S  GK  K+
Sbjct: 562 GYKLVIFTNESNIDRWKNKRQAAVDSKIGRLNSFIERVKVPIQVFIACGVSSSGGKGGKD 621

Query: 355 DPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAIGLKFYVP 386
           D +RKPK GMW +M+ HFN GI ID+D+ FYVGDAAGR  DHSDADIKFAQA GLKF+ P
Sbjct: 622 DLYRKPKAGMWQLMKKHFNSGIAIDMDKSFYVGDAAGRKMDHSDADIKFAQASGLKFFTP 681

BLAST of Lcy12g012080 vs. TAIR 10
Match: AT3G14890.2 (phosphoesterase )

HSP 1 Score: 418.7 bits (1075), Expect = 5.2e-117
Identity = 211/367 (57.49%), Postives = 264/367 (71.93%), Query Frame = 0

Query: 55  SPSSLKILAEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSDP 114
           SP S K+++EYAKS+RSTCK CS+ I AK LRLG V+R+ RGFDM +W H  C    SDP
Sbjct: 312 SPDSSKVISEYAKSSRSTCKKCSQTIAAKELRLGLVTRNFRGFDMKQWHHLGCFPVDSDP 371

Query: 115 ACSAEEINGFALLKGSDQEALKKLISR-SHEAKACKRDRDEEDG------IEERDQKK-- 174
             S E+I GF+ L+  DQ+ALK+L+ +   +    K D D +D        EE +++K  
Sbjct: 372 IVSVEDIGGFSELQSGDQDALKELVQQCGKQTLVDKMDEDNDDTEAKIKLTEETNKRKHS 431

Query: 175 --------------------------VELSTSSLQPKLDLVITASDIKNKYKDATLLPKW 234
                                     +  STS ++ + ++ ++ASD+K+KY+DA LLPKW
Sbjct: 432 EVGEMVEEDESLTKAKQQMAKTHKVNMSESTSQVEVEAEITLSASDVKDKYRDANLLPKW 491

Query: 235 KAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKRVGAEAWSLMYPSIPQKLQSLYND 294
           KAF+T+IFLE+DDGL+ S KIAAFDFDGCLA TSVK VGA+AWSLMYPSIP+KLQSL++ 
Sbjct: 492 KAFETVIFLERDDGLNDSEKIAAFDFDGCLAKTSVKIVGADAWSLMYPSIPEKLQSLHDQ 551

Query: 295 GYKLVIFTNESNIDRWKNKRQVAVDSKLGRLNNFISQVNVPIQVFVACGIGSGSGK-SKE 354
           GYKLVIFTNESNIDRWKNKRQ AVDSK+GRLN+FI +V VPIQVF+ACG+ S  GK  K+
Sbjct: 552 GYKLVIFTNESNIDRWKNKRQAAVDSKIGRLNSFIERVKVPIQVFIACGVSSSGGKGGKD 611

Query: 355 DPFRKPKPGMWHIMENHFNDGIPIDLDQCFYVGDAAGRAKDHSDADIKFAQAIGLKFYVP 386
           D +RKPK GMW +M+ HFN GI ID+D+ FYVGDAAGR  DHSDADIKFAQA GLKF+ P
Sbjct: 612 DLYRKPKAGMWQLMKKHFNSGIAIDMDKSFYVGDAAGRKMDHSDADIKFAQASGLKFFTP 671

BLAST of Lcy12g012080 vs. TAIR 10
Match: AT2G31320.1 (poly(ADP-ribose) polymerase 2 )

HSP 1 Score: 48.9 bits (115), Expect = 1.1e-05
Identity = 27/79 (34.18%), Postives = 42/79 (53.16%), Query Frame = 0

Query: 63  AEYAKSNRSTCKGCSKAIPAKALRLGFVSRDGRGFD--MTRWFHSDCASFGSDPACSAEE 122
           AEYAKS+RS+CK C   I  +  RLG + +    FD  M  W H+ C    +    S ++
Sbjct: 10  AEYAKSSRSSCKTCKSVINKENFRLGKLVQSTH-FDGIMPMWNHASCILKKTKQIKSVDD 69

Query: 123 INGFALLKGSDQEALKKLI 140
           + G   L+  DQ+ ++K +
Sbjct: 70  VEGIESLRWEDQQKIRKYV 87

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84JE87.3e-11657.49Polynucleotide 3'-phosphatase ZDP OS=Arabidopsis thaliana OX=3702 GN=ZDP PE=1 SV... [more]
Q9JLV62.0e-2838.31Bifunctional polynucleotide phosphatase/kinase OS=Mus musculus OX=10090 GN=Pnkp ... [more]
Q96T605.7e-2836.95Bifunctional polynucleotide phosphatase/kinase OS=Homo sapiens OX=9606 GN=PNKP P... [more]
O139118.6e-2436.46Bifunctional polynucleotide phosphatase/kinase OS=Schizosaccharomyces pombe (str... [more]
Q196831.9e-2337.93Uncharacterized protein F21D5.5 OS=Caenorhabditis elegans OX=6239 GN=F21D5.5 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1F9Q94.7e-18286.02polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IH481.8e-18185.75polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FAH64.4e-17289.46polynucleotide 3'-phosphatase ZDP isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3CLI91.7e-17178.83polynucleotide 3'-phosphatase ZDP OS=Cucumis melo OX=3656 GN=LOC103501815 PE=4 S... [more]
A0A6J1IFZ52.2e-17189.16polynucleotide 3'-phosphatase ZDP isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_038898753.12.1e-18484.68polynucleotide 3'-phosphatase ZDP [Benincasa hispida][more]
XP_023535258.11.1e-18284.29polynucleotide 3'-phosphatase ZDP isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022937206.19.6e-18286.02polynucleotide 3'-phosphatase ZDP isoform X1 [Cucurbita moschata][more]
XP_022976526.13.7e-18185.75polynucleotide 3'-phosphatase ZDP isoform X1 [Cucurbita maxima][more]
KAG7024794.16.5e-17882.78Polynucleotide 3'-phosphatase ZDP, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G14890.15.2e-11757.49phosphoesterase [more]
AT3G14890.25.2e-11757.49phosphoesterase [more]
AT2G31320.11.1e-0534.18poly(ADP-ribose) polymerase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001510Zinc finger, PARP-typeSMARTSM01336zf_PARP_3coord: 64..140
e-value: 4.4E-21
score: 86.0
IPR001510Zinc finger, PARP-typePFAMPF00645zf-PARPcoord: 64..139
e-value: 7.3E-13
score: 49.1
IPR001510Zinc finger, PARP-typePROSITEPS50064PARP_ZN_FINGER_2coord: 61..139
score: 19.31599
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 197..389
e-value: 4.1E-56
score: 191.2
IPR006551Polynucleotide 3'-phosphataseTIGRFAMTIGR01664TIGR01664coord: 207..382
e-value: 1.2E-57
score: 192.4
IPR036957Zinc finger, PARP-type superfamilyGENE3D3.30.1740.10coord: 55..170
e-value: 9.6E-21
score: 76.2
IPR013954Polynucleotide kinase 3 phosphatasePFAMPF08645PNK3Pcoord: 219..382
e-value: 3.7E-45
score: 153.5
IPR006549HAD-superfamily hydrolase,subfamily IIIATIGRFAMTIGR01662TIGR01662coord: 219..375
e-value: 2.2E-35
score: 119.8
NoneNo IPR availablePIRSRPIRSR004682-4PIRSR004682-4coord: 221..365
e-value: 5.1E-10
score: 37.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..167
NoneNo IPR availablePANTHERPTHR12083BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASEcoord: 53..386
NoneNo IPR availableCDDcd01625HAD_PNPcoord: 219..382
e-value: 5.18977E-71
score: 217.988
NoneNo IPR availableSUPERFAMILY57716Glucocorticoid receptor-like (DNA-binding domain)coord: 60..145
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 201..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g012080.1Lcy12g012080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
biological_process GO:0046939 nucleotide phosphorylation
biological_process GO:0098506 polynucleotide 3' dephosphorylation
cellular_component GO:0005634 nucleus
molecular_function GO:0003690 double-stranded DNA binding
molecular_function GO:0046404 polydeoxyribonucleotide 5'-hydroxyl-kinase activity
molecular_function GO:0046403 polynucleotide 3'-phosphatase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003677 DNA binding