Lcy12g012050 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g012050 vs. ExPASy Swiss-Prot
Match: P81898 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Prunus dulcis OX=3755 PE=1 SV=2)

HSP 1 Score: 793.9 bits (2049), Expect = 1.3e-228
Identity = 394/559 (70.48%), Postives = 447/559 (79.96%), Query Frame = 0

Query: 50  TPPTVFFEVTKPIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLE 109
           TPPTVFFEVTKPI  PK K CS L LQHDF YTYG++PV ANY+PPS C    FS IVLE
Sbjct: 8   TPPTVFFEVTKPIEVPKTKPCSQLILQHDFAYTYGQAPVFANYTPPSDCPSQTFSTIVLE 67

Query: 110 WKATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVY 169
           WKATCR RQFDRIFGVWLGGVEILRSCTAEPR  GIVWTV+KDITRYYSLL  NQTLAVY
Sbjct: 68  WKATCRRRQFDRIFGVWLGGVEILRSCTAEPRPNGIVWTVEKDITRYYSLLKSNQTLAVY 127

Query: 170 LGNLIDKTYTGIYHVKIHIHFYPEEKGFGDNGLYSEKLASGYGPGADLILPISRNLPLND 229
           LGNLIDKTYTGIYHV I +HFYP ++         + LASGY   ADLILPISRNLPLND
Sbjct: 128 LGNLIDKTYTGIYHVNISLHFYPAKEKLNSFQQKLDNLASGYHSWADLILPISRNLPLND 187

Query: 230 GLWFEVQNSSDVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPG 289
           GLWFEVQNS+D ++KEF+IPQNAYRAVLEVYVSFHENDEFWYSNLPN+YI ANNL+ TPG
Sbjct: 188 GLWFEVQNSNDTELKEFKIPQNAYRAVLEVYVSFHENDEFWYSNLPNEYIAANNLSGTPG 247

Query: 290 NGPFREVLVSLDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHL 349
           NGPFREV+VSLDGE+V AVWPFTVI+TGG+NPLLWRPIT IGSF+LP+YDIE+TPFLG +
Sbjct: 248 NGPFREVVVSLDGEVVGAVWPFTVIFTGGINPLLWRPITAIGSFDLPTYDIEITPFLGKI 307

Query: 350 LDGKVHNFDFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPD 409
           LDGK H F F VT+ALNVWY++ANLHLWLD  S KTEGKL  H   PL  S  S+F G +
Sbjct: 308 LDGKSHKFGFNVTNALNVWYVDANLHLWLDKQSTKTEGKLSKHSSLPLVVSLVSDFKGLN 367

Query: 410 GTFLTKVTRSVSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVY 469
           GTFLT+ +RSVSS+GWVKSS G ITT+S QDF YSNSMV+G DGN QIVNQ+I FN+SVY
Sbjct: 368 GTFLTRTSRSVSSTGWVKSSYGNITTRSIQDFYYSNSMVLGKDGNMQIVNQKIIFNDSVY 427

Query: 470 AKQDSSHVYSFKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKN 529
               SS+V+S  S KTFPLY+Y+D   +GNG+Y  + N+ LGF EKK+ GLG   SSL+N
Sbjct: 428 INLPSSYVHSLTSHKTFPLYLYTDFLGQGNGTYLLITNVDLGFIEKKS-GLGFSNSSLRN 487

Query: 530 LQNGQGIMVVKGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKK-L 589
           L++ +G MVVK +LV  GL STQQ YRY   K CY RNISSSNYTILYD + S C+KK L
Sbjct: 488 LRSAEGNMVVKNNLVVSGLESTQQIYRYDGGKFCYFRNISSSNYTILYDKVGSKCNKKSL 547

Query: 590 ESPESLRFRRWPIPARRAF 608
            + + +  R WP  AR  F
Sbjct: 548 SNLDFVLSRLWPFGARMNF 565

BLAST of Lcy12g012050 vs. ExPASy TrEMBL
Match: A0A6J1CHN7 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Momordica charantia OX=3673 GN=LOC111011511 PE=4 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 534/620 (86.13%), Postives = 572/620 (92.26%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTK 60
           MASSLS  L ISLL +L PF+SDANL KTR RRPDLLSE   TA    T PPT+FFEVTK
Sbjct: 1   MASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTK 60

Query: 61  PIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120
           PI TPKAK CS+LALQHDFGYTYGKSPVLA+Y+PPSHCSF+KFSKIVLEW ATCRGRQFD
Sbjct: 61  PIETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180

Query: 181 IYHVKIHIHFYPEEKGFGDN-GLYSEKLASGYGPGADLILPISRNLPLNDGLWFEVQNSS 240
           IYHV+IHIHFYPEEK FGDN G + EK ASGY  GADLI+PISR+LPLNDGLWFEVQ+S+
Sbjct: 181 IYHVRIHIHFYPEEKSFGDNGGRFLEKSASGYDSGADLIVPISRSLPLNDGLWFEVQSST 240

Query: 241 DVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVS 300
           DVQVKEFEIPQN YRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNGPFREVLVS
Sbjct: 241 DVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVS 300

Query: 301 LDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDF 360
           LDG++V AVWPFTV+YTGGVNPLLWRPITGIGSFNLPSYDIE+TPFLG+LLDGK HNF F
Sbjct: 301 LDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNFGF 360

Query: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRS 420
           RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIV+PL NSE+SNFTGPDGTF TKVTRS
Sbjct: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVTRS 420

Query: 421 VSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYS 480
           VSS GWVKSS GIITTQSNQDFSYSNSM +G++G+SQ+VNQ+IQFN+SVYAKQ SSHVYS
Sbjct: 421 VSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHVYS 480

Query: 481 FKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 540
           FKS+KTFPLY+YSDTKDEGNG+YQSLANLTLGFNEKKTDG GSFISSLKNLQNGQG+MVV
Sbjct: 481 FKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLMVV 540

Query: 541 KGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRW 600
           K HLVT+GLGSTQQDYRYHDDKLCY RNISSSNYTIL+DN+K+ C KK ESP SLRF RW
Sbjct: 541 KDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFGRW 600

Query: 601 PIPARRAFLDSNLLANSGHV 620
           PIPARRAFLDS+L  NS HV
Sbjct: 601 PIPARRAFLDSSLQVNSVHV 620

BLAST of Lcy12g012050 vs. ExPASy TrEMBL
Match: A0A6J1IYD9 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A-like OS=Cucurbita maxima OX=3661 GN=LOC111481042 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 532/609 (87.36%), Postives = 566/609 (92.94%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTK 60
           MASSLS  LLISLLFIL PFHS+A+L + RLR PDL SESEL ATG+ TTPPT+FFEVTK
Sbjct: 1   MASSLSSPLLISLLFILLPFHSNADLHRARLRGPDLFSESELAATGNHTTPPTLFFEVTK 60

Query: 61  PIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120
           PI TP+A+ CSLL LQHDFGYTYGKSPVLA+YSPPSHCSFSKFSKIVLEWKATCRGRQFD
Sbjct: 61  PIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRYYSLLSKNQTLAVYLGNLIDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180

Query: 181 IYHVKIHIHFYPEEKGFGDNGLYSEKLASG-YGPGADLILPISRNLPLNDGLWFEVQNSS 240
           IYHVKIHIHFYPEEK F     YSEKL SG Y   ADLILPISR+LPLNDGLWFEVQNS+
Sbjct: 181 IYHVKIHIHFYPEEKRF----QYSEKLDSGYYDSRADLILPISRSLPLNDGLWFEVQNST 240

Query: 241 DVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVS 300
           D+QVKEFEIPQN+YRAVLEVYVSFHENDEFWYSNLPNDYI ANNLT TPGNGPFREVLV+
Sbjct: 241 DIQVKEFEIPQNSYRAVLEVYVSFHENDEFWYSNLPNDYIMANNLTQTPGNGPFREVLVN 300

Query: 301 LDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDF 360
           LDGE+V A+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+F F
Sbjct: 301 LDGEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKPHHFGF 360

Query: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRS 420
            VTHALNVWYINANLHLWLDD S KTEGKLLNHI++PL NSEDSNFTGPDGTFLTK +RS
Sbjct: 361 SVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDSNFTGPDGTFLTKASRS 420

Query: 421 VSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYS 480
           VSSSGWVKSS GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFN+SVYAK +S+ VYS
Sbjct: 421 VSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKGESADVYS 480

Query: 481 FKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 540
           FKS+KTFPLYMYSDTKDEGNGS+QS+ANLTLGFNEKK DGLGSFISSLKN+QNGQG+M V
Sbjct: 481 FKSLKTFPLYMYSDTKDEGNGSFQSIANLTLGFNEKKVDGLGSFISSLKNVQNGQGMMAV 540

Query: 541 KGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRW 600
           KGHLVT+GLGSTQQDYRYHDDKLCYSRNISSSNYTILYD++KS+CDKK ESPESLRF +W
Sbjct: 541 KGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVKSLCDKKPESPESLRFGKW 600

Query: 601 PIPARRAFL 609
           P  +RR FL
Sbjct: 601 PFLSRRVFL 605

BLAST of Lcy12g012050 vs. ExPASy TrEMBL
Match: A0A6J1FLZ6 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A-like OS=Cucurbita moschata OX=3662 GN=LOC111446428 PE=4 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 527/608 (86.68%), Postives = 566/608 (93.09%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTK 60
           MASSLS  LLISLLFIL PFHS+A+L +T LRRPDL SESEL ATG+ TTPPT+FFEVTK
Sbjct: 1   MASSLSSPLLISLLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTK 60

Query: 61  PIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120
           PI TP+A+ CSLL LQHDFGYTYGKSPVLA+YSPPSHCSFSKFSKIVLEWKATCRGRQFD
Sbjct: 61  PIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180
           RIFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRYYSLLSKNQTLAVYLGNLIDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180

Query: 181 IYHVKIHIHFYPEEKGFGDNGLYSEKLASGYGPGADLILPISRNLPLNDGLWFEVQNSSD 240
           IYHVKIHIHFYPEEK F     YSEKLA+GY   ADLILPISR+LPLNDGLWFEVQNS+D
Sbjct: 181 IYHVKIHIHFYPEEKRF----QYSEKLAAGYDSRADLILPISRSLPLNDGLWFEVQNSTD 240

Query: 241 VQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVSL 300
           +QVKEFEIPQN+YRAVLEVY+SFHENDEFWYSNLPNDYI ANNL+ TPGNGPFREVLV+L
Sbjct: 241 IQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYILANNLSQTPGNGPFREVLVNL 300

Query: 301 DGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDFR 360
           D E+V A+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+F F 
Sbjct: 301 DDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGFS 360

Query: 361 VTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRSV 420
           VTHALNVWYINANLHLWLDD S KTEGKLLNHI++PL NSED NFTGPDGTFLTK +RSV
Sbjct: 361 VTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRSV 420

Query: 421 SSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYSF 480
           SSSGWVKSS GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFN+SVYAK++S HVYSF
Sbjct: 421 SSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESGHVYSF 480

Query: 481 KSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVVK 540
           KS+KTFPLYMYS+T DEGNGS+QS+ANLTLGFNEKK DGLGSFISSLKN+QNGQG+M VK
Sbjct: 481 KSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTVK 540

Query: 541 GHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRWP 600
           GHLVT+GLGSTQQDYRYHDDKLCYSRNISSSNYTILYD++ S+CDKK ESPESLRFR+WP
Sbjct: 541 GHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKWP 600

Query: 601 IPARRAFL 609
             +RR FL
Sbjct: 601 FLSRRVFL 604

BLAST of Lcy12g012050 vs. ExPASy TrEMBL
Match: A0A0A0L222 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G627190 PE=4 SV=1)

HSP 1 Score: 1062.0 bits (2745), Expect = 9.6e-307
Identity = 517/619 (83.52%), Postives = 558/619 (90.15%), Query Frame = 0

Query: 6   SYFLLIS---LLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTKPI 65
           S+ LLIS   LL +L PFHS+ANL KTRLRR DL SE ++T T D +TPPTVFFEV+KPI
Sbjct: 4   SFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKPI 63

Query: 66  VTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFDRI 125
           VTPK+K CSLL L HDF +TYGK PVLA+Y+PPSHCSF+KFSKIVLEWKATCRGRQFDRI
Sbjct: 64  VTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRI 123

Query: 126 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTGIY 185
           FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY SLLSKNQTLAVYLGNL+DKTYTGIY
Sbjct: 124 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIY 183

Query: 186 HVKIHIHFYPEEKGFGDNGLYSEKLASGYGPG--ADLILPISRNLPLNDGLWFEVQNSSD 245
           +VKI IHFYPEE+GFG NG+YS+KL S Y     ADLILPISRNLPLNDGLWFEVQNS+D
Sbjct: 184 NVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTD 243

Query: 246 VQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVSL 305
           V+ KEFEIPQN YRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNGPFREVLV L
Sbjct: 244 VEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDL 303

Query: 306 DGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDFR 365
           DGE+V AVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGKVHNF FR
Sbjct: 304 DGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFR 363

Query: 366 VTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRSV 425
           VTHALNVW+INANLHLWLDDNS KTE KLLNHIV+    S+D NFTGPDGTFLTKVTRSV
Sbjct: 364 VTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSV 423

Query: 426 SSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYSF 485
           SS+GWVKSS GIITT SNQD SYSNSM MGN+G+SQ VNQEIQFN SVYAK++SSHVYS 
Sbjct: 424 SSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSV 483

Query: 486 KSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVVK 545
           KS+KTFPLYMYS+TKDE NGSYQS+ANLTLGFNEKKTDGLG  ISSLKN+QNGQG++VVK
Sbjct: 484 KSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVVK 543

Query: 546 GHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRWP 605
           GHLVT GLGSTQQDYRYHDD  CYSRNISSSNYTIL+DN+K+ C +K  SPESLRFRRWP
Sbjct: 544 GHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRWP 603

Query: 606 IPARRAFLDSNLLANSGHV 620
           IPARRAFLDSNL  N+G V
Sbjct: 604 IPARRAFLDSNLFVNNGRV 622

BLAST of Lcy12g012050 vs. ExPASy TrEMBL
Match: A0A5D3DX64 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001000 PE=4 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 1.1e-305
Identity = 516/621 (83.09%), Postives = 564/621 (90.82%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTK 60
           MASS S+ LLISLL +L PFHSDA+L KTRLR+ DL SES ++ T D +TPPTVFFEVTK
Sbjct: 1   MASS-SFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 60

Query: 61  PIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120
           PIVTPKAK CSLL L HDF YTYGK PVLA+Y+PPSHCSF+KFSKIVLEWKATCRGRQFD
Sbjct: 61  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY SLLSKNQTLAVYLGNL+DKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 180

Query: 181 IYHVKIHIHFYPEEKGFGDNGLYSEKLASGYGPG--ADLILPISRNLPLNDGLWFEVQNS 240
           IY+VKIHIHFYPEE+GFG NG+YSEKL S Y     ADLILPISRNLPLNDGLWFEVQNS
Sbjct: 181 IYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 240

Query: 241 SDVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLV 300
           +DVQ+KEFEIPQN YRA+LEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNGPFREVLV
Sbjct: 241 TDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLV 300

Query: 301 SLDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFD 360
            LDG++VAAVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+F 
Sbjct: 301 DLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFG 360

Query: 361 FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTR 420
           FRVTHALNVW+INANLHLWLDDNS KTE KLLNHIV+    S+D NFTG DGTFLTKVTR
Sbjct: 361 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTR 420

Query: 421 SVSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVY 480
           SVSS+GWVKSS G ITT SNQD SYSN M+MGN+G+SQ VNQEI FN+SVYAKQ+SSHVY
Sbjct: 421 SVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVY 480

Query: 481 SFKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMV 540
           S K++KTFPLYMYS+T D+GNGSY+S+ANLTLGFNEKKTDGLGSFISSLKN+QNGQG+MV
Sbjct: 481 SSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMV 540

Query: 541 VKGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRR 600
           VKG+LVT GLGSTQQDYRY+DD  CYSR+ISSSNYTIL+DN+K++C +K  S ESLRFRR
Sbjct: 541 VKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRR 600

Query: 601 WPIPARRAFLDSNLLANSGHV 620
           WPIPARRA LDSNL  N+GHV
Sbjct: 601 WPIPARRASLDSNLFVNNGHV 620

BLAST of Lcy12g012050 vs. NCBI nr
Match: XP_022140996.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Momordica charantia])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 534/620 (86.13%), Postives = 572/620 (92.26%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTK 60
           MASSLS  L ISLL +L PF+SDANL KTR RRPDLLSE   TA    T PPT+FFEVTK
Sbjct: 1   MASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTK 60

Query: 61  PIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120
           PI TPKAK CS+LALQHDFGYTYGKSPVLA+Y+PPSHCSF+KFSKIVLEW ATCRGRQFD
Sbjct: 61  PIETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180

Query: 181 IYHVKIHIHFYPEEKGFGDN-GLYSEKLASGYGPGADLILPISRNLPLNDGLWFEVQNSS 240
           IYHV+IHIHFYPEEK FGDN G + EK ASGY  GADLI+PISR+LPLNDGLWFEVQ+S+
Sbjct: 181 IYHVRIHIHFYPEEKSFGDNGGRFLEKSASGYDSGADLIVPISRSLPLNDGLWFEVQSST 240

Query: 241 DVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVS 300
           DVQVKEFEIPQN YRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNGPFREVLVS
Sbjct: 241 DVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVS 300

Query: 301 LDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDF 360
           LDG++V AVWPFTV+YTGGVNPLLWRPITGIGSFNLPSYDIE+TPFLG+LLDGK HNF F
Sbjct: 301 LDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNFGF 360

Query: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRS 420
           RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIV+PL NSE+SNFTGPDGTF TKVTRS
Sbjct: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVTRS 420

Query: 421 VSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYS 480
           VSS GWVKSS GIITTQSNQDFSYSNSM +G++G+SQ+VNQ+IQFN+SVYAKQ SSHVYS
Sbjct: 421 VSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHVYS 480

Query: 481 FKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 540
           FKS+KTFPLY+YSDTKDEGNG+YQSLANLTLGFNEKKTDG GSFISSLKNLQNGQG+MVV
Sbjct: 481 FKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLMVV 540

Query: 541 KGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRW 600
           K HLVT+GLGSTQQDYRYHDDKLCY RNISSSNYTIL+DN+K+ C KK ESP SLRF RW
Sbjct: 541 KDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFGRW 600

Query: 601 PIPARRAFLDSNLLANSGHV 620
           PIPARRAFLDS+L  NS HV
Sbjct: 601 PIPARRAFLDSSLQVNSVHV 620

BLAST of Lcy12g012050 vs. NCBI nr
Match: XP_022982101.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A-like [Cucurbita maxima])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 532/609 (87.36%), Postives = 566/609 (92.94%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTK 60
           MASSLS  LLISLLFIL PFHS+A+L + RLR PDL SESEL ATG+ TTPPT+FFEVTK
Sbjct: 1   MASSLSSPLLISLLFILLPFHSNADLHRARLRGPDLFSESELAATGNHTTPPTLFFEVTK 60

Query: 61  PIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120
           PI TP+A+ CSLL LQHDFGYTYGKSPVLA+YSPPSHCSFSKFSKIVLEWKATCRGRQFD
Sbjct: 61  PIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRYYSLLSKNQTLAVYLGNLIDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180

Query: 181 IYHVKIHIHFYPEEKGFGDNGLYSEKLASG-YGPGADLILPISRNLPLNDGLWFEVQNSS 240
           IYHVKIHIHFYPEEK F     YSEKL SG Y   ADLILPISR+LPLNDGLWFEVQNS+
Sbjct: 181 IYHVKIHIHFYPEEKRF----QYSEKLDSGYYDSRADLILPISRSLPLNDGLWFEVQNST 240

Query: 241 DVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVS 300
           D+QVKEFEIPQN+YRAVLEVYVSFHENDEFWYSNLPNDYI ANNLT TPGNGPFREVLV+
Sbjct: 241 DIQVKEFEIPQNSYRAVLEVYVSFHENDEFWYSNLPNDYIMANNLTQTPGNGPFREVLVN 300

Query: 301 LDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDF 360
           LDGE+V A+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+F F
Sbjct: 301 LDGEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKPHHFGF 360

Query: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRS 420
            VTHALNVWYINANLHLWLDD S KTEGKLLNHI++PL NSEDSNFTGPDGTFLTK +RS
Sbjct: 361 SVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDSNFTGPDGTFLTKASRS 420

Query: 421 VSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYS 480
           VSSSGWVKSS GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFN+SVYAK +S+ VYS
Sbjct: 421 VSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKGESADVYS 480

Query: 481 FKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 540
           FKS+KTFPLYMYSDTKDEGNGS+QS+ANLTLGFNEKK DGLGSFISSLKN+QNGQG+M V
Sbjct: 481 FKSLKTFPLYMYSDTKDEGNGSFQSIANLTLGFNEKKVDGLGSFISSLKNVQNGQGMMAV 540

Query: 541 KGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRW 600
           KGHLVT+GLGSTQQDYRYHDDKLCYSRNISSSNYTILYD++KS+CDKK ESPESLRF +W
Sbjct: 541 KGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVKSLCDKKPESPESLRFGKW 600

Query: 601 PIPARRAFL 609
           P  +RR FL
Sbjct: 601 PFLSRRVFL 605

BLAST of Lcy12g012050 vs. NCBI nr
Match: XP_022941014.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A-like [Cucurbita moschata])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 527/608 (86.68%), Postives = 566/608 (93.09%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVTK 60
           MASSLS  LLISLLFIL PFHS+A+L +T LRRPDL SESEL ATG+ TTPPT+FFEVTK
Sbjct: 1   MASSLSSPLLISLLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTK 60

Query: 61  PIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120
           PI TP+A+ CSLL LQHDFGYTYGKSPVLA+YSPPSHCSFSKFSKIVLEWKATCRGRQFD
Sbjct: 61  PIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180
           RIFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRYYSLLSKNQTLAVYLGNLIDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180

Query: 181 IYHVKIHIHFYPEEKGFGDNGLYSEKLASGYGPGADLILPISRNLPLNDGLWFEVQNSSD 240
           IYHVKIHIHFYPEEK F     YSEKLA+GY   ADLILPISR+LPLNDGLWFEVQNS+D
Sbjct: 181 IYHVKIHIHFYPEEKRF----QYSEKLAAGYDSRADLILPISRSLPLNDGLWFEVQNSTD 240

Query: 241 VQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVSL 300
           +QVKEFEIPQN+YRAVLEVY+SFHENDEFWYSNLPNDYI ANNL+ TPGNGPFREVLV+L
Sbjct: 241 IQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYILANNLSQTPGNGPFREVLVNL 300

Query: 301 DGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDFR 360
           D E+V A+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+F F 
Sbjct: 301 DDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGFS 360

Query: 361 VTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRSV 420
           VTHALNVWYINANLHLWLDD S KTEGKLLNHI++PL NSED NFTGPDGTFLTK +RSV
Sbjct: 361 VTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRSV 420

Query: 421 SSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYSF 480
           SSSGWVKSS GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFN+SVYAK++S HVYSF
Sbjct: 421 SSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESGHVYSF 480

Query: 481 KSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVVK 540
           KS+KTFPLYMYS+T DEGNGS+QS+ANLTLGFNEKK DGLGSFISSLKN+QNGQG+M VK
Sbjct: 481 KSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTVK 540

Query: 541 GHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRWP 600
           GHLVT+GLGSTQQDYRYHDDKLCYSRNISSSNYTILYD++ S+CDKK ESPESLRFR+WP
Sbjct: 541 GHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKWP 600

Query: 601 IPARRAFL 609
             +RR FL
Sbjct: 601 FLSRRVFL 604

BLAST of Lcy12g012050 vs. NCBI nr
Match: XP_023525341.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 530/609 (87.03%), Postives = 565/609 (92.78%), Query Frame = 0

Query: 1   MASSLSY-FLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVT 60
           MASSLS   LLISLLFIL PF SDA+L + RLRRPDL SESEL ATGD TTPPT+FFEVT
Sbjct: 1   MASSLSSPLLLISLLFILLPFLSDADLHRARLRRPDLFSESELAATGDHTTPPTLFFEVT 60

Query: 61  KPIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQF 120
           KPI TP+A+ CSLL LQHDFGYTYGKSPVLA+YSPPSHCSFSKFSKIVLEWKATCRGRQF
Sbjct: 61  KPIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQF 120

Query: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYT 180
           DRIFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRYYSLLSKNQTLAVYLGNLIDKTYT
Sbjct: 121 DRIFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYT 180

Query: 181 GIYHVKIHIHFYPEEKGFGDNGLYSEKLASGYGPGADLILPISRNLPLNDGLWFEVQNSS 240
           GIYHVKIHIHFYPEEK F     YSEKLA+GY   ADLILPISR+LPLNDGLWFEVQNS+
Sbjct: 181 GIYHVKIHIHFYPEEKRF----QYSEKLAAGYDSRADLILPISRSLPLNDGLWFEVQNST 240

Query: 241 DVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVS 300
           D+QVKEFEIPQN+YRAVLEVYVSFHENDEFWYSNLPNDYI ANNLT TPGNGPFREVLV+
Sbjct: 241 DIQVKEFEIPQNSYRAVLEVYVSFHENDEFWYSNLPNDYILANNLTQTPGNGPFREVLVN 300

Query: 301 LDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDF 360
           LD E+V A+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+F F
Sbjct: 301 LDDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKPHHFGF 360

Query: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRS 420
            VTHALNVWYINANLHLWLDD S KTEGKLLNHI++PL NSED NFTGPDGTFLTK +RS
Sbjct: 361 SVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRS 420

Query: 421 VSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYS 480
           VSSSGWVKSS GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFN+SVYAK++S HVYS
Sbjct: 421 VSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESDHVYS 480

Query: 481 FKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 540
           FKS+KTFPLYMYS+T DEGNGS+QS+ANLTLGFNEKK DGLGSFISSLKN+QNGQG+MVV
Sbjct: 481 FKSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKVDGLGSFISSLKNVQNGQGMMVV 540

Query: 541 KGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRW 600
           KGHLVT+GLGSTQQDYRYHDDKLCYSRNISSSNYTILYD++ S+CDKK ES ESLRFR+W
Sbjct: 541 KGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESHESLRFRKW 600

Query: 601 PIPARRAFL 609
           P  +RR FL
Sbjct: 601 PFLSRRVFL 605

BLAST of Lcy12g012050 vs. NCBI nr
Match: KAG6607915.1 (hypothetical protein SDJN03_01257, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037442.1 hypothetical protein SDJN02_01069, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 526/609 (86.37%), Postives = 566/609 (92.94%), Query Frame = 0

Query: 1   MASSLSY-FLLISLLFILFPFHSDANLRKTRLRRPDLLSESELTATGDRTTPPTVFFEVT 60
           MASSLS   LLISLLFIL PFHS+A+L +T LRRPDL SESEL ATG+ TTPPT+FFEVT
Sbjct: 1   MASSLSSPLLLISLLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVT 60

Query: 61  KPIVTPKAKSCSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQF 120
           KPI TP+A+ CSLL LQHDFGYTYGKSPVLA+YSPPSHCSFS+FS+IVLEWKATCRGRQF
Sbjct: 61  KPIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSRFSRIVLEWKATCRGRQF 120

Query: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQTLAVYLGNLIDKTYT 180
           DRIFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRYYSLLSKNQTLAVYLGNLIDKTYT
Sbjct: 121 DRIFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYT 180

Query: 181 GIYHVKIHIHFYPEEKGFGDNGLYSEKLASGYGPGADLILPISRNLPLNDGLWFEVQNSS 240
           GIYHVKIHIHFYPEEK F     YSEKLA+GY   ADLILPISR+LPLNDGLWFEVQNS+
Sbjct: 181 GIYHVKIHIHFYPEEKRF----QYSEKLAAGYDSRADLILPISRSLPLNDGLWFEVQNST 240

Query: 241 DVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVS 300
           D+QVKEFEIPQN+YRAVLEVY+SFHENDEFWYSNLPNDYI ANNLT TPGNGPFREVLV+
Sbjct: 241 DIQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYIIANNLTQTPGNGPFREVLVN 300

Query: 301 LDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDF 360
           LD E+V A+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+F F
Sbjct: 301 LDDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGF 360

Query: 361 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRS 420
            VTHALNVWYINANLHLWLDD S KTEGKLLNHI++PL NSED NFTGPDGTFLTK +RS
Sbjct: 361 SVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRS 420

Query: 421 VSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSHVYS 480
           VSSSGWVKSS GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFN+SVYAK++S HVYS
Sbjct: 421 VSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESDHVYS 480

Query: 481 FKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 540
           FKS+KTFPLYMYS+T DEGNGS+QS+ANLTLGFNEKK DGLGSFISSLKN+QNGQG+M V
Sbjct: 481 FKSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTV 540

Query: 541 KGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKKLESPESLRFRRW 600
           KGHLVT+GLGSTQQDYRYHDDKLCYSRNISSSNYTILYD++ S+CDKK ESPESLRFR+W
Sbjct: 541 KGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKW 600

Query: 601 PIPARRAFL 609
           P  +RR FL
Sbjct: 601 PFLSRRVFL 605

BLAST of Lcy12g012050 vs. TAIR 10
Match: AT3G14920.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 675.2 bits (1741), Expect = 4.8e-194
Identity = 348/599 (58.10%), Postives = 434/599 (72.45%), Query Frame = 0

Query: 1   MASSLSYFLLISLLFILFPFHSDANLRKTRLR-RPDLLSESELTATGDRTT-PPTVFFEV 60
           M SSL   +    +F +    + ++L +TR R +P   S    ++     T  PT +FEV
Sbjct: 1   MTSSLPPLIFFFTVFFVHSLSAVSDLHETRSRFKPPQFSPLFFSSLPQNVTKSPTRYFEV 60

Query: 61  TKPIV--TPKAKS-CSLLALQHDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCR 120
            KP V   P A+  CS   L HDFGYTY K PVL+NY+ PSHCS  +FSKIVLE+K+T +
Sbjct: 61  QKPPVPNLPTAQQPCSYQILHHDFGYTYAKPPVLSNYTLPSHCSSREFSKIVLEFKSTSQ 120

Query: 121 GRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYYSLLSKNQT--LAVYLGNL 180
           GRQFDRIFGVWL GVEILRSCTAEPR  GIVW+V+KD+T+Y+SLL KN+T  L+VYLGNL
Sbjct: 121 GRQFDRIFGVWLDGVEILRSCTAEPRPNGIVWSVEKDVTKYHSLLVKNETQILSVYLGNL 180

Query: 181 IDKTYTGIYHVKIHIHFYPEEKGFGDNGLYSEKLASGYGPG-ADLILPISRNLPLNDGLW 240
           IDKTYTG+YHV +  HFY  E    D         SGY    AD+ILPISRNLPLNDGLW
Sbjct: 181 IDKTYTGVYHVDVIFHFYQSESNLQD--------VSGYSSSKADMILPISRNLPLNDGLW 240

Query: 241 FEVQNSSDVQVKEFEIPQNAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGP 300
           FE+ NS+D + KEFEIP+N YRAVLEVYVSFHENDEFWY NLPNDY+TANNL+   GNGP
Sbjct: 241 FEIVNSNDTKYKEFEIPRNVYRAVLEVYVSFHENDEFWYGNLPNDYVTANNLS-VAGNGP 300

Query: 301 FREVLVSLDGELVAAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDG 360
           FREV+VSLDG++  AVWPF V++TGG+NPLLWRPIT IGSF+LPSYDIE+TPFLG LLDG
Sbjct: 301 FREVVVSLDGDIAGAVWPFPVVFTGGINPLLWRPITAIGSFDLPSYDIEITPFLGSLLDG 360

Query: 361 KVHNFDFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTF 420
           K H   F VT+ALNVWYI+ANLHLWLD      EGK+L+   + L  S  S+F G +G F
Sbjct: 361 KTHKVGFSVTNALNVWYIDANLHLWLDQEKEIVEGKVLDFSRSSLEISSVSDFKGLNGNF 420

Query: 421 LTKVTRSVSSSGWVKSSLGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQ 480
            TK  RS++S G VKSS G I T +NQ+FSY N MV+G DGN QI++Q IQ ++ ++AK+
Sbjct: 421 TTKAKRSITSVGLVKSSHGDIITNANQEFSYENKMVLGKDGNLQIIDQLIQADDRIHAKR 480

Query: 481 DSSHVYSFKSVKTFPLYMYSDTKDEGNGSYQSLANLTLGFNEKKTDG----LGSFISSLK 540
            S  +Y+ KS+K+FP Y+ SDT ++ N +Y ++AN+++ FNE++++     + +F S L+
Sbjct: 481 ASREIYAAKSIKSFPFYLDSDTLEQQNNTYLAVANVSMAFNEERSESDKGLMRTFKSKLE 540

Query: 541 NLQNGQGIMVVKGHLVTKGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSICDKK 588
           N Q GQG+MVVK +LV  G GSTQQ Y Y     CY RNISS NYTILYD ++S+C KK
Sbjct: 541 NKQEGQGVMVVKNNLVVSGYGSTQQVYNYVGSDQCYFRNISSYNYTILYDKVESVCKKK 590

BLAST of Lcy12g012050 vs. TAIR 10
Match: AT5G05480.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.8e-100
Identity = 214/578 (37.02%), Postives = 306/578 (52.94%), Query Frame = 0

Query: 20  FHSDANLRKTRLRRPDLLSESELTATGDRTTP--PTVFFEVTKPIVTPK-AKSCSLLALQ 79
           F + A L  T + RP        +A+    +P  P  + E+ +P+ + +   SCS +  +
Sbjct: 12  FFTLAFLTATAVSRPSSPDRFLRSASTCLRSPVEPQEYEELRRPLPSDQLTPSCSHVLFR 71

Query: 80  HDFGYTYGKSPVLANYSPPSHCSFSKFSKIVLEWKATCRGRQFDRIFGVWLGGVEILRSC 139
           H F  T  + P    Y+PPS C    +S +VL+ +A   G Q+DRI G+WLGGVE+LR+ 
Sbjct: 72  HSFANTINRPPFTTPYTPPSSCISPPWSYVVLDLRAASSGDQYDRISGLWLGGVELLRTS 131

Query: 140 TAEPRATGIVWTVQKDITRYYSLLSKNQ-TLAVYLGNLIDKTYTGIYHVKIHIHFYPEEK 199
           TAEP  +GI W V+KD++RY SL  ++   + + L N+++  YTGIYH+ + + FY E  
Sbjct: 132 TAEPSPSGIFWNVRKDVSRYSSLFMRSDLNVTMMLENIVNDVYTGIYHINVTLIFY-EFN 191

Query: 200 GFGDNGLYSEKLA-----SGYGPGADLILPISRNLPLNDGLWFEVQNSSDVQVKEFEIPQ 259
               N   S +L            ADLI+P+      N G WF ++N  +   K  +IP 
Sbjct: 192 PIASNLKISNRLGFVDSQKRDQTPADLIIPVCDG--GNRGFWFMIENPRETYSKGIQIPS 251

Query: 260 NAYRAVLEVYVSFHENDEFWYSNLPNDYITANNLTDTPGNGPFREVLVSLDGELVAAVWP 319
           N  + VLE+YVSFH NDEFWYSN PN YI  NNLT   GNG +REV V +DG  V +  P
Sbjct: 252 NTRQIVLELYVSFHGNDEFWYSNPPNSYIRTNNLTTGRGNGAYREVFVKIDGRYVGSEVP 311

Query: 320 FTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFDFRVTHALNVWYI 379
           F VI+TGG+NPL W P+  IG+FNLPSYD++LTPFLG LLDGK H F F V   ++ W +
Sbjct: 312 FPVIFTGGINPLFWEPVVAIGAFNLPSYDMDLTPFLGLLLDGKSHEFAFGVNDGISYWLV 371

Query: 380 NANLHLWLDDNSAKTEGKLLNHIVTPLFNSEDSNFTGPDGTFLTKVTRSVSSSGWVKSSL 439
           +ANLHLWLD  S+  E     +                DG+F  +   S    GWV+SS 
Sbjct: 372 DANLHLWLDHGSSNVEAGSRLYDSPSRHMMRQEQLEQLDGSFKVEAEVSSEYDGWVRSSK 431

Query: 440 GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNNSVYAKQDSSH-VYSFKSVKTFPLY 499
           G +TT     F   + +    +G  + V Q ++    V    +S   V      +T+PL 
Sbjct: 432 GNLTTMVKSMFKVDSLVRFEKNGTYKRVEQRVKTERIVEVTSESGKPVNRVVHQRTYPLT 491

Query: 500 MYSDT---KDEGNGSYQSLANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVVKGHLVTK 559
           + + T         +Y  + N++   NE  +D  G  +  + + Q+ +G M V+ H V  
Sbjct: 492 VITSTLRGLTNDKDTYMLVTNVSHALNETHSD--GEALIQVYSRQDSEGWMEVEDHNVLA 551

Query: 560 GLGSTQQDYRYHDDKLCYSRNISSSNYTILYDNIKSIC 585
           G  ST+Q   Y D+  CYSR I ++N  I  D+    C
Sbjct: 552 GEASTKQSLSYIDEFSCYSRTIVAANGEIAQDSSSDSC 584