Lcy12g010040 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g010040
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionindole-3-acetate O-methyltransferase 1-like
LocationChr12: 37139919 .. 37145412 (+)
RNA-Seq ExpressionLcy12g010040
SyntenyLcy12g010040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCAGGGATTCAGAGGGGACTTAGCTGCACTCGAGTTCTCTTGAAGGATTGTTGTATCTTTTGGAGTTGAGTATCAAATAACTAAGTGCTAGAGGGAGTACACCTTCTCTTTGAACAACGTTTATTAACGTGCCTTGATACGGGTATTACAACCTTTTTCTTATCTGTATTTTATTTGGTAATGTATTTACGACGATTAATTATTTTCGACATTTTTATTCCCTAAATTTATTTCCAACAATAATTGCATTATTTTTAGGCCAAATAAAGCTTACTTGCACCCTAACTAATCTCGAAGAAAATTGATACGACATTATATGCGAAAGTTGAATCCATATTTCCAAAGTTTTTCTTTTACCTTTTTTCATTGTCTTAATGATTATCATGGTCAATGAGGTGATTAGTGCCTTATTCACATCTCATTAGCTAGATTCTTTTTCAATCAAGTCTTAATGTAGAATTTAACTCAAACATTTAAATTTCTTTACATTTCTAAAAATATAAAAAGAACTAAATCATTACAAATAGAATATCACAAAGACCAAGATGTCACAAGAGCGAAAGCATAATAACTAATAACTATACTTTAACCAAATAATTATCCCATTCTAGATATTAAGAAATTAAATTTGTACCTTTAGCTAGGGGTGTGTGTGGTCACTATTACTCGAAACTCGTGATGTCAAAAGTCATTCTAAATTCTTTATAGCTCATAACTTTGACTATTAGGCTAACCTATGATATAATTTATCAACCCGAGCATCATTTTGCTTCTACGTTTTAAACTCATGCCATTAACCTTCCCTATTCTATTAAGTTTGATTAACAAATAATTAAATTAACCAACCCTATATATAAATATTCTTAGAGAAACAGTTAATTAGTTGATGATTAGCTTTTTAGTTTGCATTAGCAGCCAAAACTATTGTTAATTAATAATATTAATTAAGAAGAGATATACAAAATATCAAAATCCGGCGGAAATCTAGGACTTTTTTTCTCTTATGCATGTGAGCCCCAATATTTATATAGATTTGTATTTTCTGCCCATCAATTTTACCTTTTTCTTATTATAATTTTTTTGTGATAAACTAATAATGCATAAAATAAAAATCTTTTAATTATTTAAAAAAAAGGAAAAAAAAGTTGACATATTAATGTACAAAAAAAAAAATATTATGATTTTTAGCCCTTTTGTTCTTTATAAATATCCAACTCTCTTAAGCCAACCACTTTTCATCCATAACTTAGCAACCTCTTGCACAAAAGCTTTCTCACCAAAAACTCTCCCTTCTCTCCAAGCTTAGAGATAAAGCTCAAGCCCTTCCCATGGCTCCCAAGGGCGACAACGTCGTCGTGTCGAACATGAAACTCGAGCGAATGCTCAGCATGAAAGGCGGCAAAGGCGAAGGAAGCTACGCCAACAACTCCCAAGCTCAGGTACTATATCCATTACTTGGCTGCATGCTGGTACGTAGGAATCTCCCTACACATCGGTATCAAATCTTGATACCCTATAACGTATTGATTTTCGTGAAATAATCTATTTTTCAAAACATACTTAAAAAGATAATGTCTTTGATACAAGATAAAATCTCAATTTTTACAACTCAATCACGAAAATAAACAATTGGACGAACACCGACCTAATAATTATAGGGCTAAATTTGGTGTGTGAAAAAAAAAAAAACAAAAACAAAAACCGCAGGCTCAACATGCGAGATCGGTGCTTCATCTTCTAAAAGAAACGTTGGACGGAGTTCATCTGAACTCGCCGGAAGTGCCGTTCGTGGTGGTGGATCTCGGCTGCAGCAGCGGCAGCAACACCGTTTACATCGTCGACGTCATCATAAAGCACATCATAAAGCGCTTCGAGGCCTTGTCCGTCGACCCGCCGGAGTTCACGGCGTTTTTCTCCGATCTCCCAGGAAATGACTTCAATACCCTCTTCCAGCTCCTCCCGCCGTTGGCCACTTACGGCGGCAGCATGGAGGAGTGCCTCGCCGCCGACAACCACCGCTCCTACTTCGCTGCCGGCGTTCCGGGCTCCTTCTACCGGAGGCTTTTCCCGGCGAGGTCCATCGACGTTTTTCACTCCGCGTTTTCCTTGCATTGGCTCTCTCAGGTTTGCCATTTAAGTTCCAAAAATTTTGCTTTTTTGTGTTTATTTTTATTTTTGTTTTTTTTTTTTTTAGTTATATATGCATGGCGATTGACATTTTAAATTAAAAAGTTTGACTTTCTTTTCTTTTCTTTTTTTTTTTTGGTTTAATTTTAATATTCCCGAGGAGTATTCAAGAAAAGCTCTTTAATTATTGGAATCTTTGGAATTTTGAAGAAAGAGAAGAGAAAAGGAAGAAAATTTTTATATGTTAATGCATGCAACGATCAATTAAATTAAATTAAATTAAATTACTTTTCCTTCTTTTCTTGATAACATTTTTTATTCATATTTTTTTAAACAATATTTGAATTTTATTATGTTTTTTTGGCTTCTGTTAGTCCCTTAATTATATTTTCAAAAAAAATTGTTTGTAACGAGAAATTTGTAATAAATTAGATTTTATTTTAGTTCTTATAATTAGTTTGCTGATATCTAGTTTCGAACTATTATCGATTCTACCACCACTAATTTTAAAGGTACTTTTAAAATTACCAATAATAGTTCTAGTTCAAATTATATTGTTAGGGAGTAACATATAATATATACTTAAAAGTGTTTAGCAAAGAAAATACGTCTGAAAAGTGCTTTTTAAGCTCGAAACTTAAAAAGCACACAAAAACACTTTAAACTTCAACAAACATCGTCACAAGCTATAGACTTCAACAAACATAGTTGAAGATACATATATATATATATATATATATGCTAAAAGCACTATTCTCGTACAAATAAACTCATATCTGTCAATTAAATAAAAACTTTGTCGATGTTTAGAGATCAAAATAGAATAAATTTCGAGACAATTTGATTTGATCTGAGTGCGTGTGGGGTCCACGACACGCAGGTACCGGAGAGCGTGGTGGATGCGAGATCGACGGCATACAACCGAGGGAGGGTGTCCATCCACGGCGCAAACGAGGCTGCGGCGGCGGCTTACCGGAAGCAGTTTCAGACGGACCTGGCTGGATTCCTCCGAGCCAGGGCCCACGAGCTGAAGAGAGGTGGGTCCATGTTCCTTGTCTGCTTGGGCCGGACCTCACTTGACCCAGCCGACCAAGGTGGGGCCGGCCTCCTCTTTGGGACCCACTTTCAGGATGCTTGGGATGATCTTGTCCAAGAGGTACCATATATTTCTAAATATTATTTAACAATCTTTTTCTTTTTAATACTTTCTTTTTTTTCTTTTTTGGAGTTTTTGTGTGTGAATTTGATTTGGGGTTATTTAAGGAAAAAAAAATTTATGTAGTTTCTAGAGTATTAATAGAACACGTTAGCTCGTTGTCTGATCATGTCATATATTCAATGGCGATGATAATTAAGATGATTTGTGTGGAATGATTAAATTGAAACTATACATAAACTGTCAACTTGAGTATAACTCAATTATTCAAGTCATATATGAATCTCTACGACCTTCACATGTTGAATTTATTAATTGAAAAAAACAAAAGCTTAAACTATAGGAACCATATTAAACTAAACTTAAACTATCTATCTAAGTATAGTTCAACTAGTAAGATATATCTCTAACTAAAAGGTAGCAAGTTTAAAACTGAGAGTAAACCTCGAGGCTAGATTAAAAAAATTTAAATTAAAGGTATCAAATTGAAATTAAGCTTAAATCATAAGAACTAAATTGAACTAAGTCTAAACCAGATAAAACATGTTCAATTACATAGGGACCAAATCAAAACTAATTAAGCCTAAACTATAAGGATTAAGTTTGCAACTTTATTATTTAGTTTGTACAAAATTCTAAAAACACTTGATAGTCCTAAAAAAAAATCTATTTAAATCAAAGAAACATTATATTCAATGAATGTTGTTTTGTTTGTTGTCTTTCTAAAAATGTTGTGCATCTTACTTCTTCCTTGAATCAAATTTAGAAGGAAAAAAAAAAAACCGACAAGTATTACAAAACAAAGAGACATTTTTGCGTGTAAGAAATTAAAAAAAAAAAAAAAAGAAAAGAAAAAAAAAATCCTCGACATAATAATCACAGCCGTTGATTGTTTTTGTTTCATTCAGAACTTTCTTACGTAAAAATGCATCTGAATAATTCCCCCACCACCTGTTTGTATCTGTCCTTTAACAGACCAAAAAGCAAAGCTCACATTTTAAAAAGCACATTAGCTAGGAGTAATGATTCGGTGTTGCCGGTCTCACTGAACCCCTTTACCACTTTTTTGATCATGGATTTCTATTGGTCATCATTAAACCCCATCATCGTTTCTAGCCGTCTGATGTCCCATCGACAACTCCATCCGTCCGTCCACTTGTCGGAAGGAGAATGATTGTGTTGGCTAGTCAAGTGGCTCCTCCCTCAATATTGTATTATTGTTCATGGAATTGCATGCATTTCCAATGCCAAGTTCATCATTTTGAATTTAGATAAGATTTTCATTACATGATTAAGGTATTTATCTTCGATTAAGAGATCATTGATTCGAATATCTAATGAATTGTTTGTTTTACAGGGTTTGATAACCAGTGAGAAACGTGATAGTTTTAACATTCCAGTATATGCACCGAGCCTGCAAGACTTCAAGGAGATGGTAGAAGCAGACGGTTCATTTTCCATAAACAAGCTTGAGGTTTTCAAAGGAGGAAGTCCATTGGTAGTGAACCAGCCAGACGACGCAGCAGAGGTCGGTCGAGCTTTGGCCAACAGTTGTCGAAGCGTTTCTGGTGTCCTCGTCGACGCCCACATCGGCGATCGGCTCAGCGAGGAGCTCTTCTCTCGAGTCGAGCGTAGAGCCACAAACCATGCCAAAGATCTCCTTGAGAAGCTTCAATTCTTTCACATTGTGGCATCACTTTCCCTTGCATAAACAAGAGACAACAAAAACAAAAACAAACAAAAAAATGCAAAATCTCTCTCTCTCTCTTTAGGGCTTTCTTTCTTCCTTTCTTCTCTCTTTTGGTGTTTGTTCTTGTCTTTTAAGGATATGTTACATAGAAAAGAAAAAGAATCAAACAGGCAAAAGAGAGAGGTCAAAGGGACTGCCATTTTCTTTGCCTTTTTTTCTTTTTTGTTCATTAATTATTGTAACTTTGTAGTGTGATGTTGTGAGTCATTTGACTGACTATGTTAGTGTAGTTATATATATATATATATGTGTTTACAGTTGATGGGCTTTATTGTTCCCCTAATTGGCTTTTTGGAATCCCCAAAAGAAATATAGAAGAAATAGAGAGATAAAGCCAAGTGTAACCCCTCAATTAGTTTCAACTTCCCTTTAGCTTACTTTCATATATAATGTCTATAAATGTCAAAAAGTCTCTTTGTATGCAATAGAAGACTTTATTATAAAAGCAAATTACTTTTCA

mRNA sequence

CGCAGGGATTCAGAGGGGACTTAGCTGCACTCGAGTTCTCTTGAAGGATTGTTGTATCTTTTGGAGTTGAGTATCAAATAACTAAGTGCTAGAGGGAGTACACCTTCTCTTTGAACAACGTTTATTAACGTGCCTTGATACGGAGATAAAGCTCAAGCCCTTCCCATGGCTCCCAAGGGCGACAACGTCGTCGTGTCGAACATGAAACTCGAGCGAATGCTCAGCATGAAAGGCGGCAAAGGCGAAGGAAGCTACGCCAACAACTCCCAAGCTCAGGCTCAACATGCGAGATCGGTGCTTCATCTTCTAAAAGAAACGTTGGACGGAGTTCATCTGAACTCGCCGGAAGTGCCGTTCGTGGTGGTGGATCTCGGCTGCAGCAGCGGCAGCAACACCGTTTACATCGTCGACGTCATCATAAAGCACATCATAAAGCGCTTCGAGGCCTTGTCCGTCGACCCGCCGGAGTTCACGGCGTTTTTCTCCGATCTCCCAGGAAATGACTTCAATACCCTCTTCCAGCTCCTCCCGCCGTTGGCCACTTACGGCGGCAGCATGGAGGAGTGCCTCGCCGCCGACAACCACCGCTCCTACTTCGCTGCCGGCGTTCCGGGCTCCTTCTACCGGAGGCTTTTCCCGGCGAGGTCCATCGACGTTTTTCACTCCGCGTTTTCCTTGCATTGGCTCTCTCAGGTACCGGAGAGCGTGGTGGATGCGAGATCGACGGCATACAACCGAGGGAGGGTGTCCATCCACGGCGCAAACGAGGCTGCGGCGGCGGCTTACCGGAAGCAGTTTCAGACGGACCTGGCTGGATTCCTCCGAGCCAGGGCCCACGAGCTGAAGAGAGGTGGGTCCATGTTCCTTGTCTGCTTGGGCCGGACCTCACTTGACCCAGCCGACCAAGGTGGGGCCGGCCTCCTCTTTGGGACCCACTTTCAGGATGCTTGGGATGATCTTGTCCAAGAGGGTTTGATAACCAGTGAGAAACGTGATAGTTTTAACATTCCAGTATATGCACCGAGCCTGCAAGACTTCAAGGAGATGGTAGAAGCAGACGGTTCATTTTCCATAAACAAGCTTGAGGTTTTCAAAGGAGGAAGTCCATTGGTAGTGAACCAGCCAGACGACGCAGCAGAGGTCGGTCGAGCTTTGGCCAACAGTTGTCGAAGCGTTTCTGGTGTCCTCGTCGACGCCCACATCGGCGATCGGCTCAGCGAGGAGCTCTTCTCTCGAGTCGAGCGTAGAGCCACAAACCATGCCAAAGATCTCCTTGAGAAGCTTCAATTCTTTCACATTGTGGCATCACTTTCCCTTGCATAAACAAGAGACAACAAAAACAAAAACAAACAAAAAAATGCAAAATCTCTCTCTCTCTCTTTAGGGCTTTCTTTCTTCCTTTCTTCTCTCTTTTGGTGTTTGTTCTTGTCTTTTAAGGATATGTTACATAGAAAAGAAAAAGAATCAAACAGGCAAAAGAGAGAGGTCAAAGGGACTGCCATTTTCTTTGCCTTTTTTTCTTTTTTGTTCATTAATTATTGTAACTTTGTAGTGTGATGTTGTGAGTCATTTGACTGACTATGTTAGTGTAGTTATATATATATATATATGTGTTTACAGTTGATGGGCTTTATTGTTCCCCTAATTGGCTTTTTGGAATCCCCAAAAGAAATATAGAAGAAATAGAGAGATAAAGCCAAGTGTAACCCCTCAATTAGTTTCAACTTCCCTTTAGCTTACTTTCATATATAATGTCTATAAATGTCAAAAAGTCTCTTTGTATGCAATAGAAGACTTTATTATAAAAGCAAATTACTTTTCA

Coding sequence (CDS)

ATGGCTCCCAAGGGCGACAACGTCGTCGTGTCGAACATGAAACTCGAGCGAATGCTCAGCATGAAAGGCGGCAAAGGCGAAGGAAGCTACGCCAACAACTCCCAAGCTCAGGCTCAACATGCGAGATCGGTGCTTCATCTTCTAAAAGAAACGTTGGACGGAGTTCATCTGAACTCGCCGGAAGTGCCGTTCGTGGTGGTGGATCTCGGCTGCAGCAGCGGCAGCAACACCGTTTACATCGTCGACGTCATCATAAAGCACATCATAAAGCGCTTCGAGGCCTTGTCCGTCGACCCGCCGGAGTTCACGGCGTTTTTCTCCGATCTCCCAGGAAATGACTTCAATACCCTCTTCCAGCTCCTCCCGCCGTTGGCCACTTACGGCGGCAGCATGGAGGAGTGCCTCGCCGCCGACAACCACCGCTCCTACTTCGCTGCCGGCGTTCCGGGCTCCTTCTACCGGAGGCTTTTCCCGGCGAGGTCCATCGACGTTTTTCACTCCGCGTTTTCCTTGCATTGGCTCTCTCAGGTACCGGAGAGCGTGGTGGATGCGAGATCGACGGCATACAACCGAGGGAGGGTGTCCATCCACGGCGCAAACGAGGCTGCGGCGGCGGCTTACCGGAAGCAGTTTCAGACGGACCTGGCTGGATTCCTCCGAGCCAGGGCCCACGAGCTGAAGAGAGGTGGGTCCATGTTCCTTGTCTGCTTGGGCCGGACCTCACTTGACCCAGCCGACCAAGGTGGGGCCGGCCTCCTCTTTGGGACCCACTTTCAGGATGCTTGGGATGATCTTGTCCAAGAGGGTTTGATAACCAGTGAGAAACGTGATAGTTTTAACATTCCAGTATATGCACCGAGCCTGCAAGACTTCAAGGAGATGGTAGAAGCAGACGGTTCATTTTCCATAAACAAGCTTGAGGTTTTCAAAGGAGGAAGTCCATTGGTAGTGAACCAGCCAGACGACGCAGCAGAGGTCGGTCGAGCTTTGGCCAACAGTTGTCGAAGCGTTTCTGGTGTCCTCGTCGACGCCCACATCGGCGATCGGCTCAGCGAGGAGCTCTTCTCTCGAGTCGAGCGTAGAGCCACAAACCATGCCAAAGATCTCCTTGAGAAGCTTCAATTCTTTCACATTGTGGCATCACTTTCCCTTGCATAA

Protein sequence

MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSPEVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRTSLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA
Homology
BLAST of Lcy12g010040 vs. ExPASy Swiss-Prot
Match: Q9FLN8 (Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 2.4e-172
Identity = 299/385 (77.66%), Postives = 336/385 (87.27%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNS- 60
           M  KGDNV V NMKLER+LSMKGGKG+ SYANNSQAQA HARS+LHLL+ETL+ VHLNS 
Sbjct: 1   MGSKGDNVAVCNMKLERLLSMKGGKGQDSYANNSQAQAMHARSMLHLLEETLENVHLNSS 60

Query: 61  -PEVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLF 120
               PF  VDLGCSSG+NTV+I+D I+KHI KRF+A  +DPPEFTAFFSDLP NDFNTLF
Sbjct: 61  ASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGIDPPEFTAFFSDLPSNDFNTLF 120

Query: 121 QLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVP 180
           QLLPPL +    MEECLAAD +RSYF AGVPGSFYRRLFPAR+ID FHSAFSLHWLSQVP
Sbjct: 121 QLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLFPARTIDFFHSAFSLHWLSQVP 180

Query: 181 ESVVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLG 240
           ESV D RS AYNRGRV IHGA E    AY++QFQ DLA FLRARA E+KRGG+MFLVCLG
Sbjct: 181 ESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAEFLRARAAEVKRGGAMFLVCLG 240

Query: 241 RTSLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEAD 300
           RTS+DP DQGGAGLLFGTHFQDAWDDLV+EGL+ +EKRD FNIPVYAPSLQDFKE+V+A+
Sbjct: 241 RTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRDGFNIPVYAPSLQDFKEVVDAN 300

Query: 301 GSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRV 360
           GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV+GVLV+AHIG+ LS +LFSRV
Sbjct: 301 GSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSVAGVLVEAHIGEELSNKLFSRV 360

Query: 361 ERRATNHAKDLLEKLQFFHIVASLS 384
           E RAT+HAKD+L  LQFFHIVASLS
Sbjct: 361 ESRATSHAKDVLVNLQFFHIVASLS 384

BLAST of Lcy12g010040 vs. ExPASy Swiss-Prot
Match: Q0J998 (Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 5.6e-145
Identity = 260/395 (65.82%), Postives = 312/395 (78.99%), Query Frame = 0

Query: 4   KGDNVVVS------NMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDG-VH 63
           KG+NV VS        KL  ML MKGG G+GSY NNSQAQA HAR +LH L+ETLD  + 
Sbjct: 9   KGENVTVSAAAAPRMKKLASMLCMKGGNGDGSYLNNSQAQALHARRMLHFLEETLDAMME 68

Query: 64  LNSPEVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNT 123
            +S +  F   DLGCS GSN+++IVDVI++ + + +E+   D PEF  FFSDLP NDFNT
Sbjct: 69  RSSSDKLFTAADLGCSCGSNSLFIVDVIVRRVSEAYESRGRDAPEFQVFFSDLPSNDFNT 128

Query: 124 LFQLLPP-LATYGGSMEECLAADN-----HRSYFAAGVPGSFYRRLFPARSIDVFHSAFS 183
           LFQLLPP LA   GS+EECLAA        R Y AAGVPG+FY RLFP  SIDVF S FS
Sbjct: 129 LFQLLPPLLAPVAGSLEECLAAGEGAATATRPYHAAGVPGTFYGRLFPGESIDVFTSTFS 188

Query: 184 LHWLSQVPESVVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGG 243
           LHWLSQVPE V D+ S AYN GRV +H A EA AAAY++QFQ DLA FLR+RA E+KRGG
Sbjct: 189 LHWLSQVPEEVGDSASPAYNGGRVFVHRATEAVAAAYKRQFQADLARFLRSRAREMKRGG 248

Query: 244 SMFLVCLGRTSLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQD 303
           +MFL CLGR+S DPADQGGAGLLFGTHFQDAWDDLVQEG++  EKRDSFNIPVYAPSLQ+
Sbjct: 249 AMFLACLGRSSGDPADQGGAGLLFGTHFQDAWDDLVQEGVVEGEKRDSFNIPVYAPSLQE 308

Query: 304 FKEMVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRL 363
           F+++V ADG+F+I++LE+ +GGSPLVV++PDDAAEVGRA+ANSC++V+GVLVDAHIG+R 
Sbjct: 309 FRDVVRADGAFAIDRLELVRGGSPLVVDRPDDAAEVGRAMANSCKAVAGVLVDAHIGERR 368

Query: 364 SEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 386
             +LF R+ERRA  HA++L+EK+ FFH+V SLSLA
Sbjct: 369 GAQLFERLERRAARHARELVEKMHFFHVVCSLSLA 403

BLAST of Lcy12g010040 vs. ExPASy Swiss-Prot
Match: Q5XF78 (Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.2e-59
Identity = 141/379 (37.20%), Postives = 200/379 (52.77%), Query Frame = 0

Query: 15  LERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSPEVPFVVVDLGCSSG 74
           L R+ +M+GG+ + SY NNS +QA        +L  +L  + L S + P  + DLGC++G
Sbjct: 18  LHRVFAMQGGEDDLSYVNNSDSQALAITLSKPILISSLQSIKLFSDQTPIKITDLGCATG 77

Query: 75  SNTVYIVDVIIKHIIKRFEAL--SVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSME 134
           SNT   VD +++ + +R+ A       PEF AFF DLP NDFN LF+LL           
Sbjct: 78  SNTFTTVDTVVETLQRRYTARCGGGGSPEFEAFFCDLPSNDFNMLFKLL----------A 137

Query: 135 ECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRG 194
           E    D+   YFA GV GSFY RLFP  +I V  S  +LHWLSQ+PE V++  S  +N+G
Sbjct: 138 EKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWLSQIPEKVLEKESRTWNKG 197

Query: 195 RVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRTSLDPADQGG--- 254
           +  I GA +    AY +Q   DL  F+  R  E+ +GG +F++  GR S   +  G    
Sbjct: 198 KTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFVLMAGRPSGSSSQFGDQDT 257

Query: 255 -AGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGSFSINKLEV 314
            A   F T  + AW DL++EGLI  E RD FNIP Y  S ++    ++  G F I K++ 
Sbjct: 258 RAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEEVTAGIDRCGGFKIGKMDF 317

Query: 315 FKGGSPLVVNQPD--------DAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 374
            K     +V   D        D    GRA  N  ++    +VDA++G  LS ELF R E 
Sbjct: 318 LK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMVDAYLGPDLSHELFKRYEN 377

Query: 375 RATNHAKDLLEKLQFFHIV 380
           R + + ++ L    F+ +V
Sbjct: 378 RVSTN-QEFLHITCFYGVV 380

BLAST of Lcy12g010040 vs. ExPASy Swiss-Prot
Match: A0A061DFA8 (Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 PE=3 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 2.2e-56
Identity = 137/355 (38.59%), Postives = 194/355 (54.65%), Query Frame = 0

Query: 15  LERMLSMKGGKGEGSYANNSQAQAQ-HARSVLHLLKETLDGVHLNSPEVPFVVVDLGCSS 74
           +E++LSM GG GE SYANNS  Q+   +RS+  L +  LD    N PE    + DLGCSS
Sbjct: 1   MEQILSMNGGSGEQSYANNSIGQSTCLSRSMPVLKQAVLDSCCTNLPET-VTMADLGCSS 60

Query: 75  GSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEE 134
           G NT Y V  I   I KR   L    PEF  F +DLPGNDFNT+FQ LP    Y G + E
Sbjct: 61  GPNTFYAVSEITSIIYKRCCQLGRSAPEFRVFLNDLPGNDFNTVFQSLP---AYQGKLRE 120

Query: 135 CLAADNHRSY---FAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYN 194
               +N   +   + AGVPGSFY RLFP +++   HS+ SLHWLSQVP  + D  +   N
Sbjct: 121 ----ENGPGFGPLYVAGVPGSFYGRLFPLKTLHFVHSSSSLHWLSQVPPELTDKANPLIN 180

Query: 195 RGRVSIHGAN-EAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRTSLDPADQGG 254
           + ++ I   +  A  ++Y  QF+ D + FL+ R+ E+   G M L   GRT+ DP     
Sbjct: 181 KWKIFISKTSPPAVISSYLTQFRKDFSLFLKLRSEEVAPEGRMVLTFRGRTTADPTSD-- 240

Query: 255 AGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGSFSINKLEVF 314
              L   +   A+ DLV EGL+  E+ D++N P Y P  ++ +  +E +GSF++++LEV 
Sbjct: 241 ESCLLWDYLGQAFQDLVAEGLVEEERLDTYNTPYYEPHPEEIQAEIEKEGSFTLDRLEVI 300

Query: 315 -------KGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSR 358
                   GG        DD A   + +A + R+V+  +  +H G+ + + LF R
Sbjct: 301 ALPWDSVNGGI------KDDRATTAKRMAKAIRAVNESMFRSHFGEDILDPLFHR 339

BLAST of Lcy12g010040 vs. ExPASy Swiss-Prot
Match: A4ZDG8 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 OS=Petunia hybrida OX=4102 GN=BSMT3 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.5e-52
Identity = 132/375 (35.20%), Postives = 203/375 (54.13%), Query Frame = 0

Query: 13  MKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSPEVPFVVVDLGCS 72
           M++  +L M GG G+ SYANNS  Q +       + ++ +  ++ +       + DLGCS
Sbjct: 1   MEVVEVLHMNGGNGDSSYANNSLVQQKVILMTKPITEQAMIDLYSSLFPETLCIADLGCS 60

Query: 73  SGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSME 132
            G+NT  +V  I+K + K  +      PEF   F+DLPGNDFNTLFQ L       G+ +
Sbjct: 61  LGANTFLVVSQIVKIVEKERKKHGFKSPEFYFHFNDLPGNDFNTLFQSL-------GAFQ 120

Query: 133 ECLAADNHRSY---FAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAY 192
           E L      S+   F +GVPGSFY RLFP++S+   +S++SL WLSQVP  + +      
Sbjct: 121 EDLRKHIGESFGPCFFSGVPGSFYTRLFPSKSLHFVYSSYSLMWLSQVPNGIEN------ 180

Query: 193 NRGRVSIHGANE-AAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRTSLDPADQG 252
           N+G + +   +  +   AY KQ++ D + FL+ R+ EL +GG M L  LGR S DP  + 
Sbjct: 181 NKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEELMKGGKMVLTLLGRESEDPTSK- 240

Query: 253 GAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGSFSINKLEV 312
                       A ++LV+EGLI  EK D+FNIP Y PS  + K +VE +GSF+IN+LE 
Sbjct: 241 -ECCYIWELLAMALNELVKEGLIKEEKVDAFNIPQYTPSPAEVKYLVEKEGSFTINRLET 300

Query: 313 FKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVERRATNHAKD 372
               S +  N  ++    G  ++   R+V+  L+ +H    L + +F + E   ++    
Sbjct: 301 ----SRVHWNASNNVKNGGYNVSRCMRAVAEPLLVSHFDKELMDLVFHKYEEIISDCMSK 354

Query: 373 LLEKLQFFHIVASLS 384
             EK +F +++ SL+
Sbjct: 361 --EKTEFINVIVSLT 354

BLAST of Lcy12g010040 vs. ExPASy TrEMBL
Match: A0A6J1F711 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442937 PE=4 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 7.2e-212
Identity = 372/385 (96.62%), Postives = 380/385 (98.70%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYANNSQAQAQHARSVLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCS GSNT+YI+DVIIKHIIKRFEAL+VDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFL ARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI+SEKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. ExPASy TrEMBL
Match: A0A6J1IFI9 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476788 PE=4 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 8.8e-210
Identity = 368/385 (95.58%), Postives = 379/385 (98.44%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNV+VSN+KLERMLSMKGGKGE SYANNSQAQAQHARSVLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVIVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCS GSNT+YIVDVIIKHI+KRFEAL++DPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIVKRFEALALDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAA+NHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAANNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFL ARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI+SEKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALA SCRSVSGVLVDAHIGDRLSEELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALAISCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. ExPASy TrEMBL
Match: A0A5D3DHL8 (Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00180 PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 5.4e-207
Identity = 366/385 (95.06%), Postives = 373/385 (96.88%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGE SYANNSQAQAQHARS+LHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           EVPFVVVDLGCS GSNT+YIVDVIIKHIIKRFEAL+VDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPE+
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRV IHGANEAAA AYRKQFQTDLA FL ARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAEFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI++EKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELF RVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. ExPASy TrEMBL
Match: A0A6J1FQ01 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445917 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 7.0e-207
Identity = 364/385 (94.55%), Postives = 376/385 (97.66%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSNMKLERMLSMKGGKGEGSYA+NSQAQAQHARS+LHLLKETLDGVHL+S 
Sbjct: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           +VPFVVVDLGCS GSNT+YIVDVIIKHIIKRFEAL+VDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLA+YG SMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVDARSTAYNRGRV IHGANEAAAAAYRKQFQTDLAGFL+ARA ELKR GSMFLVCLGRT
Sbjct: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI++EKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGR LANSCRSVSGVLVDAHIGDRL +ELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRVLANSCRSVSGVLVDAHIGDRLGDELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. ExPASy TrEMBL
Match: A0A0A0K2W3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 9.1e-207
Identity = 364/385 (94.55%), Postives = 373/385 (96.88%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           M PKGDNVVVSNMKLERMLSMKGGKGE SYANNSQAQAQHARS+LHLLKETLDGVHLNSP
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E PFVVVDLGCS GSNT+YI+DVIIKHIIKRFEAL+VDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EEPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSID+FHSAFSLHWLSQVPE+
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDLFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RS AYNRGRV IHGANEAAA AYRKQFQTDLAGFL ARA ELKRGGSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAGFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI++EKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELF RVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. NCBI nr
Match: XP_022936266.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 746.1 bits (1925), Expect = 1.5e-211
Identity = 372/385 (96.62%), Postives = 380/385 (98.70%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYANNSQAQAQHARSVLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCS GSNT+YI+DVIIKHIIKRFEAL+VDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFL ARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI+SEKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. NCBI nr
Match: KAG7024938.1 (Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 746.1 bits (1925), Expect = 1.5e-211
Identity = 372/385 (96.62%), Postives = 380/385 (98.70%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYANNSQAQAQHARSVLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCS GSNT+YIVDVIIKHI+KRFEAL+VDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIVKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFL ARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI+SEKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. NCBI nr
Match: XP_023536087.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 745.7 bits (1924), Expect = 1.9e-211
Identity = 371/385 (96.36%), Postives = 380/385 (98.70%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNV+VSN+KLERMLSMKGGKGE SYANNSQAQAQHARSVLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVIVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCS GSNT+YI+DVIIKHIIKRFEAL+VDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFL ARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI+SEKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. NCBI nr
Match: KAG6592061.1 (Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 745.7 bits (1924), Expect = 1.9e-211
Identity = 371/385 (96.36%), Postives = 380/385 (98.70%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYANNSQAQAQHARSVLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCS GSNT+YI+DVIIKHI+KRFEAL+VDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIVKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFL ARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI+SEKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. NCBI nr
Match: XP_022976362.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 739.2 bits (1907), Expect = 1.8e-209
Identity = 368/385 (95.58%), Postives = 379/385 (98.44%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60
           MAPKGDNV+VSN+KLERMLSMKGGKGE SYANNSQAQAQHARSVLHLLKETLDGVHLNSP
Sbjct: 1   MAPKGDNVIVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  EVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQL 120
           E+PFVVVDLGCS GSNT+YIVDVIIKHI+KRFEAL++DPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIVDVIIKHIVKRFEALALDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLATYGGSMEECLAA+NHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAANNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRT 240
           VVD RSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFL ARAHELKRGGSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGS 300
           SLDP DQGGAGLLFGTHFQDAWDDLVQEGLI+SEKRDSFNIPVYAPSLQDFKE+VEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALA SCRSVSGVLVDAHIGDRLSEELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALAISCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 386
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Lcy12g010040 vs. TAIR 10
Match: AT5G55250.1 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 606.3 bits (1562), Expect = 1.7e-173
Identity = 299/385 (77.66%), Postives = 336/385 (87.27%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNS- 60
           M  KGDNV V NMKLER+LSMKGGKG+ SYANNSQAQA HARS+LHLL+ETL+ VHLNS 
Sbjct: 1   MGSKGDNVAVCNMKLERLLSMKGGKGQDSYANNSQAQAMHARSMLHLLEETLENVHLNSS 60

Query: 61  -PEVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLF 120
               PF  VDLGCSSG+NTV+I+D I+KHI KRF+A  +DPPEFTAFFSDLP NDFNTLF
Sbjct: 61  ASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGIDPPEFTAFFSDLPSNDFNTLF 120

Query: 121 QLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVP 180
           QLLPPL +    MEECLAAD +RSYF AGVPGSFYRRLFPAR+ID FHSAFSLHWLSQVP
Sbjct: 121 QLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLFPARTIDFFHSAFSLHWLSQVP 180

Query: 181 ESVVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLG 240
           ESV D RS AYNRGRV IHGA E    AY++QFQ DLA FLRARA E+KRGG+MFLVCLG
Sbjct: 181 ESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAEFLRARAAEVKRGGAMFLVCLG 240

Query: 241 RTSLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEAD 300
           RTS+DP DQGGAGLLFGTHFQDAWDDLV+EGL+ +EKRD FNIPVYAPSLQDFKE+V+A+
Sbjct: 241 RTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRDGFNIPVYAPSLQDFKEVVDAN 300

Query: 301 GSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRV 360
           GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV+GVLV+AHIG+ LS +LFSRV
Sbjct: 301 GSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSVAGVLVEAHIGEELSNKLFSRV 360

Query: 361 ERRATNHAKDLLEKLQFFHIVASLS 384
           E RAT+HAKD+L  LQFFHIVASLS
Sbjct: 361 ESRATSHAKDVLVNLQFFHIVASLS 384

BLAST of Lcy12g010040 vs. TAIR 10
Match: AT5G55250.2 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 547.7 bits (1410), Expect = 7.2e-156
Identity = 268/346 (77.46%), Postives = 303/346 (87.57%), Query Frame = 0

Query: 40  HARSVLHLLKETLDGVHLNS--PEVPFVVVDLGCSSGSNTVYIVDVIIKHIIKRFEALSV 99
           HARS+LHLL+ETL+ VHLNS     PF  VDLGCSSG+NTV+I+D I+KHI KRF+A  +
Sbjct: 2   HARSMLHLLEETLENVHLNSSASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGI 61

Query: 100 DPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 159
           DPPEFTAFFSDLP NDFNTLFQLLPPL +    MEECLAAD +RSYF AGVPGSFYRRLF
Sbjct: 62  DPPEFTAFFSDLPSNDFNTLFQLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLF 121

Query: 160 PARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAG 219
           PAR+ID FHSAFSLHWLSQVPESV D RS AYNRGRV IHGA E    AY++QFQ DLA 
Sbjct: 122 PARTIDFFHSAFSLHWLSQVPESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAE 181

Query: 220 FLRARAHELKRGGSMFLVCLGRTSLDPADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRD 279
           FLRARA E+KRGG+MFLVCLGRTS+DP DQGGAGLLFGTHFQDAWDDLV+EGL+ +EKRD
Sbjct: 182 FLRARAAEVKRGGAMFLVCLGRTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRD 241

Query: 280 SFNIPVYAPSLQDFKEMVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 339
            FNIPVYAPSLQDFKE+V+A+GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV
Sbjct: 242 GFNIPVYAPSLQDFKEVVDANGSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSV 301

Query: 340 SGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLS 384
           +GVLV+AHIG+ LS +LFSRVE RAT+HAKD+L  LQFFHIVASLS
Sbjct: 302 AGVLVEAHIGEELSNKLFSRVESRATSHAKDVLVNLQFFHIVASLS 346

BLAST of Lcy12g010040 vs. TAIR 10
Match: AT5G56300.1 (gibberellic acid methyltransferase 2 )

HSP 1 Score: 231.9 bits (590), Expect = 8.8e-61
Identity = 141/379 (37.20%), Postives = 200/379 (52.77%), Query Frame = 0

Query: 15  LERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSPEVPFVVVDLGCSSG 74
           L R+ +M+GG+ + SY NNS +QA        +L  +L  + L S + P  + DLGC++G
Sbjct: 18  LHRVFAMQGGEDDLSYVNNSDSQALAITLSKPILISSLQSIKLFSDQTPIKITDLGCATG 77

Query: 75  SNTVYIVDVIIKHIIKRFEAL--SVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSME 134
           SNT   VD +++ + +R+ A       PEF AFF DLP NDFN LF+LL           
Sbjct: 78  SNTFTTVDTVVETLQRRYTARCGGGGSPEFEAFFCDLPSNDFNMLFKLL----------A 137

Query: 135 ECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRG 194
           E    D+   YFA GV GSFY RLFP  +I V  S  +LHWLSQ+PE V++  S  +N+G
Sbjct: 138 EKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWLSQIPEKVLEKESRTWNKG 197

Query: 195 RVSIHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRTSLDPADQGG--- 254
           +  I GA +    AY +Q   DL  F+  R  E+ +GG +F++  GR S   +  G    
Sbjct: 198 KTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFVLMAGRPSGSSSQFGDQDT 257

Query: 255 -AGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGSFSINKLEV 314
            A   F T  + AW DL++EGLI  E RD FNIP Y  S ++    ++  G F I K++ 
Sbjct: 258 RAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEEVTAGIDRCGGFKIGKMDF 317

Query: 315 FKGGSPLVVNQPD--------DAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 374
            K     +V   D        D    GRA  N  ++    +VDA++G  LS ELF R E 
Sbjct: 318 LK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMVDAYLGPDLSHELFKRYEN 377

Query: 375 RATNHAKDLLEKLQFFHIV 380
           R + + ++ L    F+ +V
Sbjct: 378 RVSTN-QEFLHITCFYGVV 380

BLAST of Lcy12g010040 vs. TAIR 10
Match: AT5G38020.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.2e-52
Identity = 133/375 (35.47%), Postives = 193/375 (51.47%), Query Frame = 0

Query: 18  MLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSPEVP--FVVVDLGCSSGS 77
           +LSMKGG GE SYANNS+ Q + A     ++ ET+  + + + + P    V DLGCSSG 
Sbjct: 3   VLSMKGGDGEHSYANNSEGQKRLASDAKPVVVETVKEMIVKT-DFPGCIKVADLGCSSGE 62

Query: 78  NTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECL 137
           NT+ ++  I+  II  ++    + PE     +DLP NDFNT F+L+P       +  + L
Sbjct: 63  NTLLVMSEIVNTIITSYQQKGKNLPEINCCLNDLPDNDFNTTFKLVP-------AFHKLL 122

Query: 138 AADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRGRVS 197
             D     F +GVPGSFY RLFP++S+   HS+  LHWLS+VP+ + D +   Y R    
Sbjct: 123 KMDVKGKCFISGVPGSFYSRLFPSKSLHFVHSSLCLHWLSKVPDGLEDNKKNVYLRSPCP 182

Query: 198 IHGANEAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRTSLDPADQGGAGLLFG 257
            +        +Y  QF+ D + FLR RA E    G M L  +GR SLDP  +        
Sbjct: 183 PN-----VYKSYLTQFKNDFSLFLRLRADETVPNGRMALTFVGRKSLDPLSKD--CFQNW 242

Query: 258 THFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGSFSINKLEV------- 317
           +   D+  DLV EG++     DSFN+P Y P   + +E++E++GSF I+  E        
Sbjct: 243 SSISDSLLDLVSEGIVKESDVDSFNLPFYNPDESEVREVIESEGSFKISNFETIFGLLFS 302

Query: 318 FKGGSPLVVNQPDD-----AAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVERRAT 377
           +K G   V +  D+       EV R  A+  RS++  ++ AH GD + + LF R      
Sbjct: 303 YKTGRTEVKDDDDNLDQSCRFEVIRKRASIIRSITEPMLGAHFGDAIMDRLFERYTYHLA 362

BLAST of Lcy12g010040 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 200.3 bits (508), Expect = 2.8e-51
Identity = 134/364 (36.81%), Postives = 195/364 (53.57%), Query Frame = 0

Query: 13  MKLERMLSMKGGKGEGSYANNSQAQAQHARSVLHLLKETLDGVHLNSPEVPFV-VVDLGC 72
           M++ R+L M  G GE SYA NS AQ+        ++ E L  + +++ E+  + + DLGC
Sbjct: 1   MEVMRVLHMNKGNGETSYAKNSTAQSNIISLGRRVMDEALKKLMMSNSEISSIGIADLGC 60

Query: 73  SSGSNTVYIVDVIIKHIIKRFEALSVDPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSM 132
           SSG N++  +  I+  I      L    PE     +DLP NDFN +   LP       + 
Sbjct: 61  SSGPNSLLSISNIVDTIHNLCPDLDRPVPELRVSLNDLPSNDFNYICASLPEFYDRVNNN 120

Query: 133 EECL--AADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVP--ESVVDARST 192
           +E L        S F + VPGSFY RLFP RS+   HS+ SLHWLSQVP  E+  + R+ 
Sbjct: 121 KEGLGFGRGGGESCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQVPCREAEKEDRTI 180

Query: 193 A---YNRGRVSIHGAN-EAAAAAYRKQFQTDLAGFLRARAHELKRGGSMFLVCLGRTSLD 252
                N G++ I   + ++A  AY  QFQTD   FLR+R+ EL  GG M L  LGR SLD
Sbjct: 181 TADLENMGKIYISKTSPKSAHKAYALQFQTDFWVFLRSRSEELVPGGRMVLSFLGRRSLD 240

Query: 253 PADQGGAGLLFGTHFQDAWDDLVQEGLITSEKRDSFNIPVYAPSLQDFKEMVEADGSFSI 312
           P  +             A   + +EG+I  EK D+FN P YA S ++ K ++E +GSFSI
Sbjct: 241 PTTE--ESCYQWELLAQALMSMAKEGIIEEEKIDAFNAPYYAASSEELKMVIEKEGSFSI 300

Query: 313 NKLEV----FKGGS------PLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEE 358
           ++LE+    ++GGS       LV+    +A   GR ++N+ R+V   +++   G+ + +E
Sbjct: 301 DRLEISPIDWEGGSISEESYDLVIRSKPEALASGRRVSNTIRAVVEPMLEPTFGENVMDE 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLN82.4e-17277.66Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 ... [more]
Q0J9985.6e-14565.82Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5XF781.2e-5937.20Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE... [more]
A0A061DFA82.2e-5638.59Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 P... [more]
A4ZDG82.5e-5235.20S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3... [more]
Match NameE-valueIdentityDescription
A0A6J1F7117.2e-21296.62indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IFI98.8e-21095.58indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A5D3DHL85.4e-20795.06Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1FQ017.0e-20794.55indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A0A0K2W39.1e-20794.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022936266.11.5e-21196.62indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata][more]
KAG7024938.11.5e-21196.62Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. a... [more]
XP_023536087.11.9e-21196.36indole-3-acetate O-methyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6592061.11.9e-21196.36Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022976362.11.8e-20995.58indole-3-acetate O-methyltransferase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G55250.11.7e-17377.66IAA carboxylmethyltransferase 1 [more]
AT5G55250.27.2e-15677.46IAA carboxylmethyltransferase 1 [more]
AT5G56300.18.8e-6137.20gibberellic acid methyltransferase 2 [more]
AT5G38020.11.2e-5235.47S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G19640.12.8e-5136.81jasmonic acid carboxyl methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005299SAM dependent carboxyl methyltransferasePFAMPF03492Methyltransf_7coord: 62..383
e-value: 5.6E-105
score: 351.3
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 10..384
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 33..312
e-value: 2.0E-122
score: 410.3
NoneNo IPR availablePANTHERPTHR31009:SF42INDOLE-3-ACETATE O-METHYLTRANSFERASE 1coord: 10..384
IPR042086Methyltransferase, alpha-helical capping domainGENE3D1.10.1200.270coord: 18..367
e-value: 2.0E-122
score: 410.3
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 13..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g010040.1Lcy12g010040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity