Lcy12g009260 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g009260
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionE3 ubiquitin-protein ligase
LocationChr12: 35958489 .. 35961565 (-)
RNA-Seq ExpressionLcy12g009260
SyntenyLcy12g009260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAAGAAGAAATTGTTTTGTTTGTTGGGTTTGCGTTTTGGATTAGCTCTCCACGTTGCCATTTCAAATGGGGCGGGCCACTCTGAGCTTCCCCCTTCTTCAACAATGGTGTGTGGCCAACCGCCATTCCCTCTTTGCTTTAAATATCTGTCTATGAGGGCCACTCCGCCCGACACCCCCTTCAATCTTGCTAGTTGGATTTGGTGGCCAACCATTCACCATTGAAAACCACTTTCAATTCAATCATCTCTCTCTCTGTCTCTCTCTCTCTCTCTCTCTCTCTCTGTTTCTTTCTTTTCCTTATCCATTTGAAACCCCATGTGGGGTTTTGTTTATATGTGTGTGTTTTGAATCTTGCAGGAGTATCTGGGTTGGTCTTTAATGGGGGTTGGAAGGATTGATTGGGACAGAGGAAGATAGATATTCGCGGTTCAACTGTTGAAGAAAAACGGTTGATTATCTCTTCTTCTCTACTTGGGGATTTTGTTTTGTTTTGTATTTGAAGTTAGGCCAACGCCTTTGGCGTATTTCTAAGCCGTCTGATTGTTGTTTTCACTTGTAATCAAAGTTAGGATATCTTCTTTGGTTTCTCTGTTTTTCTTGATTTTCTGGTGACCCTCTGCGACCCAACTTCACCTTCCTGTCTTCTTCTTTTCCAACCGTTTTTTGTTGCCTTCTCTTGCTCTTTTTGCTTCTATATACACTCTACTCCCAATTCTTTGTTTGTTCTGAGATGCCCTTTTAAAGCTGCTTTCTGAATTGGCAGTTTTGGGCCTGCACGATAGGTGTTTGTTGTTTTGCCTCACTGAATTCCTTGTAGTTGGGACATGGGACTTTCATGAATTTGAATTCTTTGCTTTTGTCTCAAAGTGGTTTTCGTTTCTTCTTTAATCATTTCGGGCAAAACTTGTGTGATTTTGGTTGTCTTCGTGCTGGGACTGGCCCTTCAGTTTCAGTTATCCTTTCATGAGAACCACCCCCCTGTTCTTTGCTCATCTTCCAACTTTTTTCATGTCGGTGTAGTTTGTTCTTGATATCGCGAGCCTGGTTGGTTATAGTTGGAAAAGGTTATTTTGCAATGATTCATTCTGGTTTCTTCCTTGCGGGTCCTTCATTTAATTCTGATGCTACTACTTCTTTCATATCAACTCCCTTGTCTGGAGAAGATGGCGCTACAGCTGATGATATTAGAAACGATACCTCTGATTCCCTCCCCCCTTCTTTCTGGGTTAGAACGGCGATGAAAATATCTAGAGCCAGATGGTTCATTTTCTTGAGAAGAGTTTTCCACTACCAGAACGGATCGAGATCTGATCTTGGGCCGAATCCTTTCAATTCGGGTTCGTGGATGGCGATGGAACTTGTAGCTCTGTTGTTTCAGCTAATCATCTCAGTGCTTACTCTTGCCATTTCACAAACTGAGAGGCCAGTCTGGCCTATGAGATTGTGGATAGGTGGTTATGATCTTGGCTGTGTTCTCAGCTTACTGCTTCTCTATGGTCGCTATTGGTATCATTATCTAATACAGGGAGATGGTAACGGTCTGTTGGATATCGAACAGCAGAGGAGCAATGATTCATCTAGGTAATCTTAAACTTGCTACTTTCCAGTTTCCTCTTAATTGATTTTTAGCTGGAATCATAATCGTGAAATATGTAAGACTCTTAACCTTGTCCTTAGAATGGACAAAGATGCTTATGGTCAATCATGTTCTAACATTCTTTTCCCTTAGTGTTTTGGGGTAAAAATGCATGCAGTTCATGAGTTTTTGCTTTGCAATACTCAGAAACTGAGTAGAAGAATAGAAGGTTTTACAATGACCCACAGAATTAAAAGTTCTTATATTGTTTGAGTAACTGAATATAAGTCAAAAGCAGACTTGGGAGCTCAACTGAAAGTTGAACCCAATATTTTATTGGGTTGCTTTGTCTTAAACATTCAAAAAGTCTGCATATGCAAAACTTTACTCTTCCTGCAAAACTTTACCTCTAATCTGACTACTTTTATTCAACATAATTCAGGTACTCACATTTGATGAACAAATGCCGCACGTCTCTCGATCTTTTCTTCGCAATATGGTTTGTAATGGGCAATCTTTGGGTCTTCGACTCTCGCTTTGCCTCGTTCCAAAAAGCTCCCAAGCTACATCTACTATGCAGCTTCCTGCTAGTTTGGAATGCCATCTGCTACTCTTTCCCATTCATTCTGTTTCTGCTGCTATGTTGCTGTGTGCCATTGATTAGCAGTCTCATAGGATATAACATTAACATGGGGTCTACCGAAAAAGGAGCATTGGACGATCAGATTTCCCAGCTACCTTGCTGGAGATACAAAGCAGTCGAGGCCAACATCAACCCCGGAAGTCAACCGGATAATAACAATACAGGACTTCCAAAAGAGGATCCAGTAAGTAATACCTTTTTGGCTGGTTTAAGAAAAACTACAGAAAGAAAGTCGAAAAAGAACCATTTCTTTTTTGCCCTGATCGATCGGTAATTAAAATGCTTTCACTTGCCTTTGATTTCTCAGGAATGTTGCATTTGCCTTGCCAAGTATATAGACAAGGAAGAAGTGAGACAGTTGCCTTGTTCCCATGTGTTTCATCTCAAGTGTGTAGATAAGTGGCTTGCTATTACATCTTCATGCCCTCTCTGTAAGCAACAACTTCAAAGATAGAGAGCCATGAAAGCTGCTTTTTGTTTTTCTTTTCATTTTCCTTTTCTTTTGCCCTCCAAAAGAAAATTTTCTCATTTACACTATTTTGTGATTTAGTCATTTAGATTAGAGTCTCAACATGGTTACTTGAGTGAGTTCTTGTGCTCACCAGTTACCTAAGTATACTGAAATACTGAATGTTATATACAGCAGGAAAAATATGCCTTATTTGTAGATATTGTGAGAACTATAGTATTTGCCTAATGAATAGAAACCAAGTGTGTTTTTTTTTTCCACACTTTGTGCCTTGAACTTATTGTTAGCGTTTACTGCTGCCCGTTCCCGTCCCGTCCCCGTCCCGACCCAACTTGATTGTCTGTGTAAAATTTGTGTATGGAACAAGAAGAAAACTCGAAAAATC

mRNA sequence

GAAGAAGAAGAAATTGTTTTGTTTGTTGGGTTTGCGTTTTGGATTAGCTCTCCACGTTGCCATTTCAAATGGGGCGGGCCACTCTGAGCTTCCCCCTTCTTCAACAATGGTGTGTGGCCAACCGCCATTCCCTCTTTGCTTTAAATATCTGTCTATGAGGGCCACTCCGCCCGACACCCCCTTCAATCTTGCTAGTTGGATTTGGTGGCCAACCATTCACCATTGAAAACCACTTTCAATTCAATCATCTCTCTCTCTGAGTATCTGGGTTGGTCTTTAATGGGGGTTGGAAGGATTGATTGGGACAGAGGAAGATAGATATTCGCGGTTCAACTGTTGAAGAAAAACGGTTGATTATCTCTTCTTCTCTACTTGGGGATTTTGTTTTGTTTTGTATTTGAAGTTAGGCCAACGCCTTTGGCGTATTTCTAAGCCGTCTGATTGTTGTTTTCACTTGTAATCAAAGTTAGGATATCTTCTTTGGTTTCTCTGTTTTTCTTGATTTTCTGGTGACCCTCTGCGACCCAACTTCACCTTCCTGTCTTCTTCTTTTCCAACCGTTTTTTGTTGCCTTCTCTTGCTCTTTTTGCTTCTATATACACTCTACTCCCAATTCTTTGTTTGTTCTGAGATGCCCTTTTAAAGCTGCTTTCTGAATTGGCAGTTTTGGGCCTGCACGATAGGTGTTTGTTGTTTTGCCTCACTGAATTCCTTGTAGTTGGGACATGGGACTTTCATGAATTTGAATTCTTTGCTTTTGTCTCAAAGTGGTTTTCGTTTCTTCTTTAATCATTTCGGGCAAAACTTGTGTGATTTTGGTTGTCTTCGTGCTGGGACTGGCCCTTCAGTTTCAGTTATCCTTTCATGAGAACCACCCCCCTGTTCTTTGCTCATCTTCCAACTTTTTTCATGTCGGTGTAGTTTGTTCTTGATATCGCGAGCCTGGTTGGTTATAGTTGGAAAAGGTTATTTTGCAATGATTCATTCTGGTTTCTTCCTTGCGGGTCCTTCATTTAATTCTGATGCTACTACTTCTTTCATATCAACTCCCTTGTCTGGAGAAGATGGCGCTACAGCTGATGATATTAGAAACGATACCTCTGATTCCCTCCCCCCTTCTTTCTGGGTTAGAACGGCGATGAAAATATCTAGAGCCAGATGGTTCATTTTCTTGAGAAGAGTTTTCCACTACCAGAACGGATCGAGATCTGATCTTGGGCCGAATCCTTTCAATTCGGGTTCGTGGATGGCGATGGAACTTGTAGCTCTGTTGTTTCAGCTAATCATCTCAGTGCTTACTCTTGCCATTTCACAAACTGAGAGGCCAGTCTGGCCTATGAGATTGTGGATAGGTGGTTATGATCTTGGCTGTGTTCTCAGCTTACTGCTTCTCTATGGTCGCTATTGGTATCATTATCTAATACAGGGAGATGGTAACGGTCTGTTGGATATCGAACAGCAGAGGAGCAATGATTCATCTAGGTACTCACATTTGATGAACAAATGCCGCACGTCTCTCGATCTTTTCTTCGCAATATGGTTTGTAATGGGCAATCTTTGGGTCTTCGACTCTCGCTTTGCCTCGTTCCAAAAAGCTCCCAAGCTACATCTACTATGCAGCTTCCTGCTAGTTTGGAATGCCATCTGCTACTCTTTCCCATTCATTCTGTTTCTGCTGCTATGTTGCTGTGTGCCATTGATTAGCAGTCTCATAGGATATAACATTAACATGGGGTCTACCGAAAAAGGAGCATTGGACGATCAGATTTCCCAGCTACCTTGCTGGAGATACAAAGCAGTCGAGGCCAACATCAACCCCGGAAGTCAACCGGATAATAACAATACAGGACTTCCAAAAGAGGATCCAGAATGTTGCATTTGCCTTGCCAAGTATATAGACAAGGAAGAAGTGAGACAGTTGCCTTGTTCCCATGTGTTTCATCTCAAGTGTGTAGATAAGTGGCTTGCTATTACATCTTCATGCCCTCTCTGTAAGCAACAACTTCAAAGATAGAGAGCCATGAAAGCTGCTTTTTGTTTTTCTTTTCATTTTCCTTTTCTTTTGCCCTCCAAAAGAAAATTTTCTCATTTACACTATTTTGTGATTTAGTCATTTAGATTAGAGTCTCAACATGGTTACTTGAGTGAGTTCTTGTGCTCACCAGTTACCTAAGTATACTGAAATACTGAATGTTATATACAGCAGGAAAAATATGCCTTATTTGTAGATATTGTGAGAACTATAGTATTTGCCTAATGAATAGAAACCAAGTGTGTTTTTTTTTTCCACACTTTGTGCCTTGAACTTATTGTTAGCGTTTACTGCTGCCCGTTCCCGTCCCGTCCCCGTCCCGACCCAACTTGATTGTCTGTGTAAAATTTGTGTATGGAACAAGAAGAAAACTCGAAAAATC

Coding sequence (CDS)

ATGATTCATTCTGGTTTCTTCCTTGCGGGTCCTTCATTTAATTCTGATGCTACTACTTCTTTCATATCAACTCCCTTGTCTGGAGAAGATGGCGCTACAGCTGATGATATTAGAAACGATACCTCTGATTCCCTCCCCCCTTCTTTCTGGGTTAGAACGGCGATGAAAATATCTAGAGCCAGATGGTTCATTTTCTTGAGAAGAGTTTTCCACTACCAGAACGGATCGAGATCTGATCTTGGGCCGAATCCTTTCAATTCGGGTTCGTGGATGGCGATGGAACTTGTAGCTCTGTTGTTTCAGCTAATCATCTCAGTGCTTACTCTTGCCATTTCACAAACTGAGAGGCCAGTCTGGCCTATGAGATTGTGGATAGGTGGTTATGATCTTGGCTGTGTTCTCAGCTTACTGCTTCTCTATGGTCGCTATTGGTATCATTATCTAATACAGGGAGATGGTAACGGTCTGTTGGATATCGAACAGCAGAGGAGCAATGATTCATCTAGGTACTCACATTTGATGAACAAATGCCGCACGTCTCTCGATCTTTTCTTCGCAATATGGTTTGTAATGGGCAATCTTTGGGTCTTCGACTCTCGCTTTGCCTCGTTCCAAAAAGCTCCCAAGCTACATCTACTATGCAGCTTCCTGCTAGTTTGGAATGCCATCTGCTACTCTTTCCCATTCATTCTGTTTCTGCTGCTATGTTGCTGTGTGCCATTGATTAGCAGTCTCATAGGATATAACATTAACATGGGGTCTACCGAAAAAGGAGCATTGGACGATCAGATTTCCCAGCTACCTTGCTGGAGATACAAAGCAGTCGAGGCCAACATCAACCCCGGAAGTCAACCGGATAATAACAATACAGGACTTCCAAAAGAGGATCCAGAATGTTGCATTTGCCTTGCCAAGTATATAGACAAGGAAGAAGTGAGACAGTTGCCTTGTTCCCATGTGTTTCATCTCAAGTGTGTAGATAAGTGGCTTGCTATTACATCTTCATGCCCTCTCTGTAAGCAACAACTTCAAAGATAG

Protein sequence

MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSNDSSRYSHLMNKCRTSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR
Homology
BLAST of Lcy12g009260 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.9e-27
Identity = 79/262 (30.15%), Postives = 126/262 (48.09%), Query Frame = 0

Query: 104 ISVLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGRY----WYHYLIQGDGNGLLDI 163
           I V  L +S+ E+P  P+R+W+ GY + C L +  +   Y       +   G G+GL + 
Sbjct: 123 IGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYRRRRRRRHPEDGGGSGLTNS 182

Query: 164 EQQ------RSNDSSRYSHLMNKCRTSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLL 223
             Q      +  D    S+      ++  +F  IW+++G  WV          +P+L+ L
Sbjct: 183 SSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFYWVSAGGQTLSSDSPQLYWL 242

Query: 224 CSFLLVWN----AICYSFPFILFLLLCCCVPLISSLIGYNINMGSTEKGALDDQISQLPC 283
           C   L ++      C +   ++ L +CCC+P I +++ Y +   + ++GA  + I Q+P 
Sbjct: 243 CIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAIL-YAV---ADQEGASKNDIDQMPK 302

Query: 284 WRYKAVEANINPGSQPDNN-----------NTGLPKEDPECCICLAKYIDKEEVRQLPCS 341
           +R+     N+   S                   L  ED ECCICL +Y D  E+R+LPC+
Sbjct: 303 FRFTKT-GNVEKLSGKARGIMTECGTDSPIERSLSPEDAECCICLCEYEDGVELRELPCN 362

BLAST of Lcy12g009260 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 9.5e-27
Identity = 80/268 (29.85%), Postives = 129/268 (48.13%), Query Frame = 0

Query: 102 LIISVLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGRYWYHYLIQ----------G 161
           ++ S + L  +  ERP  P+R+WI  Y L C+  ++L++  YW     +           
Sbjct: 89  VLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVWSEYWRRNSTRRARDLESYDHE 148

Query: 162 DGNGLLDIEQQRSNDSSRYSHLMNKCRTSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLH 221
           D N   D EQ   ++S+ YS  + +C +   +   IW+++G  WV +       +AP L+
Sbjct: 149 DYNIEYDYEQDSDDNSTTYS-FVKRCESINTVISFIWWIIGFYWVVEGGDKLLGEAPNLY 208

Query: 222 LLCSFLLV----WNAICYSFPFILFLLLCCCVPLISSLIGYNINMGSTEKGALDDQISQL 281
            L    L     +   C     ++ + LCCC+P I +L+     +  TE G  + ++  L
Sbjct: 209 WLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCIIALL---YAVAGTE-GVSEAELGVL 268

Query: 282 PCWRYKAVEAN----INPGSQ---PDN-----NNTGLPKEDPECCICLAKYIDKEEVRQL 341
           P +++KA  +N      PG     P N         L  ED +CCICL+ Y D  E+  L
Sbjct: 269 PLYKFKAFHSNEKNITGPGKMVPIPINGLCLATERTLLAEDADCCICLSSYEDGAELHAL 328

Query: 342 PCSHVFHLKCVDKWLAITSSCPLCKQQL 344
           PC+H FH  C+ KWL + ++CPLCK  +
Sbjct: 329 PCNHHFHSTCIVKWLKMRATCPLCKYNI 351

BLAST of Lcy12g009260 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 1.8e-25
Identity = 82/303 (27.06%), Postives = 142/303 (46.86%), Query Frame = 0

Query: 91  MAMELVALLFQLIISVLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGRYWYH---- 150
           + ++ V  L  ++++   L +S  E P  P+R+WI GY L C++ ++ +   Y       
Sbjct: 75  VVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNSRR 134

Query: 151 -----------------YLIQGDGNGL--------LDIEQQRSNDSSRYSHLMNKCRTSL 210
                             + + +G GL        L++ Q  + ++S   HL     ++ 
Sbjct: 135 RRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHL----ESAN 194

Query: 211 DLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLC----SFLLVWNAICYSFPFILFLLLCC 270
            +   IW+V+G  WV        Q +P+L+ LC     F + +   C +   ++ + +CC
Sbjct: 195 TMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAVCC 254

Query: 271 CVPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANI-----NPGSQPDNNNT-- 330
           C+P I +++ Y +   + ++GA  + I QL  ++++ V   +         Q D+     
Sbjct: 255 CLPCIIAVL-YAV---AEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMT 314

Query: 331 ----------GLPKEDPECCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCK 344
                      LP ED ECCICL+ Y D+ E+R+LPC H FH  CVDKWL I ++CPLCK
Sbjct: 315 ECGTDSPVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCK 369

BLAST of Lcy12g009260 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-24
Identity = 77/301 (25.58%), Postives = 141/301 (46.84%), Query Frame = 0

Query: 91  MAMELVALLFQLIISVLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGRYWYHYLIQ 150
           + +++V  L  + ++   L +S+ E P+ P+R+W+ GY L CVL ++ +   Y      +
Sbjct: 101 VVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYRRRNRRR 160

Query: 151 GDGNGLLDIEQQRSNDSSRYSHLMNK-----------------------------CRTSL 210
            +        + RS+ SS  S  + +                               ++ 
Sbjct: 161 TNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAKHLESAN 220

Query: 211 DLFFAIWFVMGNLWVFDSRFASFQKAPKLHLL----CSFLLVWNAICYSFPFILFLLLCC 270
            +F  IW+++G  WV        Q++P+++ L      F + +   C +   ++ + +CC
Sbjct: 221 TMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCC 280

Query: 271 CVPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAV-EANINPGSQPDNNNTG----- 330
           C+P I +++ Y +   + ++GA  + I QL  ++++ + +AN +   +      G     
Sbjct: 281 CLPCIIAVL-YAV---ADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTEC 340

Query: 331 ---------LPKEDPECCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQ 344
                    L +ED ECCICL+ Y D  E+R+LPC H FH  CVDKWL I ++CPLCK  
Sbjct: 341 GTDSPIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLCKYN 397

BLAST of Lcy12g009260 vs. ExPASy Swiss-Prot
Match: Q9FL07 (RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.3e-11
Identity = 32/81 (39.51%), Postives = 46/81 (56.79%), Query Frame = 0

Query: 264 ISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECCICLAKYIDKEEVRQLP-CSHVFH 323
           I  LP + YK +      GS       G  +E  +C +CL ++ +K+++R LP CSH FH
Sbjct: 113 IDALPVFHYKEI-----VGSAGGGGGNGAAQEPFDCAVCLCEFSEKDKLRLLPMCSHAFH 172

Query: 324 LKCVDKWLAITSSCPLCKQQL 344
           L C+D WL   S+CPLC+  L
Sbjct: 173 LNCIDTWLQSNSTCPLCRGTL 188

BLAST of Lcy12g009260 vs. ExPASy TrEMBL
Match: A0A1S3CI94 (E3 ubiquitin-protein ligase At4g11680 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501269 PE=4 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.7e-183
Identity = 314/347 (90.49%), Postives = 328/347 (94.52%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFL+GPSFNSDATTSFIS  +SGEDGA ADDIRN+ SDS+PPSFWV+ AMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSGEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIISV TLAISQ E+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISVFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDI--EQQRSNDSSRYSHLMNKCR 180
           MRLWIGGYDLGCVLSLLLLYGR+ YHYL+QG+ N L DI  EQQRSN+SSRYSHLMN+CR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQGNDNRLSDIEHEQQRSNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLWVFDSR ASFQ+APKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPE 300
           VPLISSL GYNINMGS+EKGA DDQISQLPCWRYKAVEANINP SQPDN+NTGL KEDPE
Sbjct: 241 VPLISSLTGYNINMGSSEKGASDDQISQLPCWRYKAVEANINPRSQPDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 347

BLAST of Lcy12g009260 vs. ExPASy TrEMBL
Match: A0A0A0K4H0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G278230 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.9e-181
Identity = 310/347 (89.34%), Postives = 324/347 (93.37%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFL+GPSFNSDATTSFIS  +S EDGA ADDIRN+ SDS+PPSFWV+ AMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIIS  TLAISQ E+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDI--EQQRSNDSSRYSHLMNKCR 180
           MRLWIGGYDLGCVLSLLLLYGR+ YHYL+Q DGN L DI  EQQR+N+SSRYSHLMN+CR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLW FDSR ASFQ+APKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPE 300
           VPLISSL GYNIN GSTEKGA DDQISQLPCWRYKAVEANINP SQ DN+NTGL KEDPE
Sbjct: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           CCICLAKYIDKEEVRQLPCSHVFHL+CVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 347

BLAST of Lcy12g009260 vs. ExPASy TrEMBL
Match: A0A6J1FHW3 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC111445910 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.9e-181
Identity = 305/345 (88.41%), Postives = 323/345 (93.62%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFLAGPSFNSDATTSFIS+ +SGEDGATADD RNDTS SLPPSFWVRTAM++SRA
Sbjct: 1   MIHSGFFLAGPSFNSDATTSFISSTVSGEDGATADDTRNDTSGSLPPSFWVRTAMRVSRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISV TLAISQTE+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVFTLAISQTEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSNDSSRYSHLMNKCRTS 180
           MRLWI GYDLGCVLSLLLL GR+   YL+QGD N L DIE Q SN+SSRYSHL+N+CRT 
Sbjct: 121 MRLWIAGYDLGCVLSLLLLCGRHRCRYLMQGDSNSLSDIEHQSSNESSRYSHLINRCRTF 180

Query: 181 LDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVP 240
           LDLFFAIWFVMGNLW+FDSRFASFQ APKLHLLCSF+LVWNAICYSFPF LFLLLCCCVP
Sbjct: 181 LDLFFAIWFVMGNLWIFDSRFASFQGAPKLHLLCSFVLVWNAICYSFPFFLFLLLCCCVP 240

Query: 241 LISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECC 300
           +ISSLIGYNINMGSTEKGALDDQISQLPCW+YK V+ NIN G QPDN+NTGLPKEDPECC
Sbjct: 241 VISSLIGYNINMGSTEKGALDDQISQLPCWKYKVVDVNINLGIQPDNSNTGLPKEDPECC 300

Query: 301 ICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           ICLAKYIDKE+VRQLPCSHVFHL+CVDKWLAI SSCPLCKQQLQR
Sbjct: 301 ICLAKYIDKEDVRQLPCSHVFHLRCVDKWLAIASSCPLCKQQLQR 345

BLAST of Lcy12g009260 vs. ExPASy TrEMBL
Match: A0A6J1CCU0 (E3 ubiquitin-protein ligase At1g63170 OS=Momordica charantia OX=3673 GN=LOC111010402 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.3e-180
Identity = 309/345 (89.57%), Postives = 325/345 (94.20%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFL+GPSFNSDATTSFIS  +S +DGATADDIRN+TSDSLPPSFWVRTAMK SRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSEDDGATADDIRNETSDSLPPSFWVRTAMKASRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNS +WMAMELVALLFQLIISV TLAISQTERPVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSSAWMAMELVALLFQLIISVFTLAISQTERPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSNDSSRYSHLMNKCRTS 180
           MRLWIGGYDLGC+LSLLLLYGRY Y YL+QGDGN LLDIEQQRSN+SSRYSHL NKCRT 
Sbjct: 121 MRLWIGGYDLGCLLSLLLLYGRYRYLYLMQGDGNSLLDIEQQRSNESSRYSHLTNKCRTL 180

Query: 181 LDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVP 240
           L+LFFAIWFVMGNLWVFDSRF SFQ+APKLHLLCS LLVWNAICYSFPFILFLLLCCCVP
Sbjct: 181 LELFFAIWFVMGNLWVFDSRFGSFQRAPKLHLLCSILLVWNAICYSFPFILFLLLCCCVP 240

Query: 241 LISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECC 300
           LISSLIGYN+NMGSTEKGA DDQISQLP WRYKAV++NI PG+QPD ++TG PKEDPECC
Sbjct: 241 LISSLIGYNMNMGSTEKGASDDQISQLPSWRYKAVDSNIKPGNQPD-SHTGPPKEDPECC 300

Query: 301 ICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           ICLAKY DKEEVRQLPCSHVFH KCVDKWLAITSSCPLCKQQLQR
Sbjct: 301 ICLAKYKDKEEVRQLPCSHVFHQKCVDKWLAITSSCPLCKQQLQR 344

BLAST of Lcy12g009260 vs. ExPASy TrEMBL
Match: A0A6J1J2C9 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111480623 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.2e-179
Identity = 301/345 (87.25%), Postives = 320/345 (92.75%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFLAGPSFNSDATTSFIS+ +SGEDGATADD RNDTS SLPP FWV TAM++SRA
Sbjct: 1   MIHSGFFLAGPSFNSDATTSFISSTVSGEDGATADDTRNDTSGSLPPPFWVGTAMRVSRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALL  LIISV TLAISQTE+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLCHLIISVFTLAISQTEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSNDSSRYSHLMNKCRTS 180
           MRLWI GYDLGCVLSLLLLYGR+   YL+QGD N L DIE Q SN+SSRYSHL+N+CRT 
Sbjct: 121 MRLWIAGYDLGCVLSLLLLYGRHRCRYLMQGDSNSLSDIEHQSSNESSRYSHLINRCRTF 180

Query: 181 LDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVP 240
           LDLFFAIWFVMGNLW+FDSRFASFQ+APKLHLLCSF+LVWNAICYSFPF LFLLLCCCVP
Sbjct: 181 LDLFFAIWFVMGNLWIFDSRFASFQRAPKLHLLCSFVLVWNAICYSFPFFLFLLLCCCVP 240

Query: 241 LISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECC 300
           +ISS IGYNINMGSTEKGALDDQISQLPCW+YK V+ NIN G QPDN+NTGLPKEDPECC
Sbjct: 241 VISSFIGYNINMGSTEKGALDDQISQLPCWKYKVVDVNINLGIQPDNSNTGLPKEDPECC 300

Query: 301 ICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           ICLAKYIDKE+VRQLPCSHVFHL+CVDKWLAI SSCPLCKQQLQR
Sbjct: 301 ICLAKYIDKEDVRQLPCSHVFHLRCVDKWLAIASSCPLCKQQLQR 345

BLAST of Lcy12g009260 vs. NCBI nr
Match: XP_038899145.1 (E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida])

HSP 1 Score: 664.1 bits (1712), Expect = 6.7e-187
Identity = 318/346 (91.91%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFL+GPSFNSD TTSFIS P+SGEDGA AD+I N TSDS+PPSFWVR AMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDVTTSFISIPVSGEDGARADEISNHTSDSIPPSFWVRMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRR+FHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIIS  TLAISQ ERPVWP
Sbjct: 61  RWFIFLRRIFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISAFTLAISQAERPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDI--EQQRSNDSSRYSHLMNKCR 180
           MRLWIGGYDLGCVLSLLLLYGR+ YHYL QGDGN L DI  EQQRSN+SSRYSHLMNKCR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLTQGDGNSLSDIEHEQQRSNESSRYSHLMNKCR 180

Query: 181 TSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLWVFDSR ASFQ+APKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPE 300
           VPL SSLIGYNINMGSTEKGALDDQISQLPCWRYKAVE NINPGSQPDN+NTGLPKEDPE
Sbjct: 241 VPLTSSLIGYNINMGSTEKGALDDQISQLPCWRYKAVETNINPGSQPDNSNTGLPKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQ 345
           CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQ
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQ 346

BLAST of Lcy12g009260 vs. NCBI nr
Match: XP_008463025.1 (PREDICTED: E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis melo])

HSP 1 Score: 651.7 bits (1680), Expect = 3.4e-183
Identity = 314/347 (90.49%), Postives = 328/347 (94.52%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFL+GPSFNSDATTSFIS  +SGEDGA ADDIRN+ SDS+PPSFWV+ AMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSGEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIISV TLAISQ E+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISVFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDI--EQQRSNDSSRYSHLMNKCR 180
           MRLWIGGYDLGCVLSLLLLYGR+ YHYL+QG+ N L DI  EQQRSN+SSRYSHLMN+CR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQGNDNRLSDIEHEQQRSNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLWVFDSR ASFQ+APKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPE 300
           VPLISSL GYNINMGS+EKGA DDQISQLPCWRYKAVEANINP SQPDN+NTGL KEDPE
Sbjct: 241 VPLISSLTGYNINMGSSEKGASDDQISQLPCWRYKAVEANINPRSQPDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 347

BLAST of Lcy12g009260 vs. NCBI nr
Match: KAG7037312.1 (E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 643.7 bits (1659), Expect = 9.3e-181
Identity = 305/345 (88.41%), Postives = 323/345 (93.62%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFLAGPSFNSDATTSFIS+ +SGEDGATADD RNDTS SLPPSFWVRTAM++SRA
Sbjct: 1   MIHSGFFLAGPSFNSDATTSFISSTVSGEDGATADDTRNDTSGSLPPSFWVRTAMRVSRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISV TLAISQTE+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVFTLAISQTEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSNDSSRYSHLMNKCRTS 180
           MRLWI GYDLGCVLSLLLL GR+   YL+QGD N L DIE Q SN+SSRYSHL+N+CRT 
Sbjct: 121 MRLWIAGYDLGCVLSLLLLCGRHQCRYLMQGDSNSLSDIEHQSSNESSRYSHLINRCRTF 180

Query: 181 LDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVP 240
           LDLFFAIWFVMGNLW+FDSRFASFQ APKLHLLCSF+LVWNAICYSFPF LFLLLCCCVP
Sbjct: 181 LDLFFAIWFVMGNLWIFDSRFASFQGAPKLHLLCSFVLVWNAICYSFPFFLFLLLCCCVP 240

Query: 241 LISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECC 300
           +ISSLIGYNINMGSTEKGALDDQISQLPCW+YK V+ NIN G QPDN+NTGLPKEDPECC
Sbjct: 241 VISSLIGYNINMGSTEKGALDDQISQLPCWKYKVVDVNINLGIQPDNSNTGLPKEDPECC 300

Query: 301 ICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           ICLAKYIDKE+VRQLPCSHVFHL+CVDKWLAI SSCPLCKQQLQR
Sbjct: 301 ICLAKYIDKEDVRQLPCSHVFHLRCVDKWLAIASSCPLCKQQLQR 345

BLAST of Lcy12g009260 vs. NCBI nr
Match: XP_011659087.1 (E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis sativus])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 310/347 (89.34%), Postives = 324/347 (93.37%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFL+GPSFNSDATTSFIS  +S EDGA ADDIRN+ SDS+PPSFWV+ AMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIIS  TLAISQ E+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDI--EQQRSNDSSRYSHLMNKCR 180
           MRLWIGGYDLGCVLSLLLLYGR+ YHYL+Q DGN L DI  EQQR+N+SSRYSHLMN+CR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLW FDSR ASFQ+APKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPE 300
           VPLISSL GYNIN GSTEKGA DDQISQLPCWRYKAVEANINP SQ DN+NTGL KEDPE
Sbjct: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           CCICLAKYIDKEEVRQLPCSHVFHL+CVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 347

BLAST of Lcy12g009260 vs. NCBI nr
Match: XP_022940226.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 305/345 (88.41%), Postives = 323/345 (93.62%), Query Frame = 0

Query: 1   MIHSGFFLAGPSFNSDATTSFISTPLSGEDGATADDIRNDTSDSLPPSFWVRTAMKISRA 60
           MIHSGFFLAGPSFNSDATTSFIS+ +SGEDGATADD RNDTS SLPPSFWVRTAM++SRA
Sbjct: 1   MIHSGFFLAGPSFNSDATTSFISSTVSGEDGATADDTRNDTSGSLPPSFWVRTAMRVSRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISV TLAISQTE+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVFTLAISQTEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSNDSSRYSHLMNKCRTS 180
           MRLWI GYDLGCVLSLLLL GR+   YL+QGD N L DIE Q SN+SSRYSHL+N+CRT 
Sbjct: 121 MRLWIAGYDLGCVLSLLLLCGRHRCRYLMQGDSNSLSDIEHQSSNESSRYSHLINRCRTF 180

Query: 181 LDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVP 240
           LDLFFAIWFVMGNLW+FDSRFASFQ APKLHLLCSF+LVWNAICYSFPF LFLLLCCCVP
Sbjct: 181 LDLFFAIWFVMGNLWIFDSRFASFQGAPKLHLLCSFVLVWNAICYSFPFFLFLLLCCCVP 240

Query: 241 LISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECC 300
           +ISSLIGYNINMGSTEKGALDDQISQLPCW+YK V+ NIN G QPDN+NTGLPKEDPECC
Sbjct: 241 VISSLIGYNINMGSTEKGALDDQISQLPCWKYKVVDVNINLGIQPDNSNTGLPKEDPECC 300

Query: 301 ICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 346
           ICLAKYIDKE+VRQLPCSHVFHL+CVDKWLAI SSCPLCKQQLQR
Sbjct: 301 ICLAKYIDKEDVRQLPCSHVFHLRCVDKWLAIASSCPLCKQQLQR 345

BLAST of Lcy12g009260 vs. TAIR 10
Match: AT5G55970.1 (RING/U-box superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 2.6e-120
Identity = 202/301 (67.11%), Postives = 241/301 (80.07%), Query Frame = 0

Query: 46  PPSFWVRTAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIIS 105
           P SF++R AMK+SRARWFIFLRRVFHYQNGSRSDLG NPFNS +WM  EL+ALL QL + 
Sbjct: 47  PSSFYIRLAMKVSRARWFIFLRRVFHYQNGSRSDLGSNPFNSSTWMMSELIALLVQLTVI 106

Query: 106 VLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIE-QQRS 165
             TLAIS+ ERP+WP+RLWI GYD+GC+L+L+LLYGRY    + QG+G  L D+E QQR 
Sbjct: 107 TFTLAISKEERPIWPVRLWITGYDVGCLLNLMLLYGRYRQLDINQGNGFVLGDVEQQQRG 166

Query: 166 NDSSRYSHLMNKCRTSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAIC 225
            + +R SHLMNKCRTSL+LFFAIWFV+GN+WVFDSRF SF  APKLH+LC  LL WNAIC
Sbjct: 167 REETRSSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGSFHHAPKLHVLCVSLLAWNAIC 226

Query: 226 YSFPFILFLLLCCCVPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQ 285
           YSFPF+LFL LCC VPLISSL+GYN+NMGS+++ A DDQIS LP W++K     I+  + 
Sbjct: 227 YSFPFLLFLFLCCLVPLISSLLGYNMNMGSSDRAASDDQISSLPSWKFK----RIDDSAS 286

Query: 286 PDNNNTGLPKEDPECCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQ 345
             ++++    +DPECCICLAKY DKEEVR+LPCSH FH KCVD+WL I S CPLCKQ L 
Sbjct: 287 DSDSDSATVTDDPECCICLAKYKDKEEVRKLPCSHKFHSKCVDQWLRIISCCPLCKQDLP 343

BLAST of Lcy12g009260 vs. TAIR 10
Match: AT5G55970.2 (RING/U-box superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 2.6e-120
Identity = 202/301 (67.11%), Postives = 241/301 (80.07%), Query Frame = 0

Query: 46  PPSFWVRTAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIIS 105
           P SF++R AMK+SRARWFIFLRRVFHYQNGSRSDLG NPFNS +WM  EL+ALL QL + 
Sbjct: 47  PSSFYIRLAMKVSRARWFIFLRRVFHYQNGSRSDLGSNPFNSSTWMMSELIALLVQLTVI 106

Query: 106 VLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGRYWYHYLIQGDGNGLLDIE-QQRS 165
             TLAIS+ ERP+WP+RLWI GYD+GC+L+L+LLYGRY    + QG+G  L D+E QQR 
Sbjct: 107 TFTLAISKEERPIWPVRLWITGYDVGCLLNLMLLYGRYRQLDINQGNGFVLGDVEQQQRG 166

Query: 166 NDSSRYSHLMNKCRTSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAIC 225
            + +R SHLMNKCRTSL+LFFAIWFV+GN+WVFDSRF SF  APKLH+LC  LL WNAIC
Sbjct: 167 REETRSSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGSFHHAPKLHVLCVSLLAWNAIC 226

Query: 226 YSFPFILFLLLCCCVPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQ 285
           YSFPF+LFL LCC VPLISSL+GYN+NMGS+++ A DDQIS LP W++K     I+  + 
Sbjct: 227 YSFPFLLFLFLCCLVPLISSLLGYNMNMGSSDRAASDDQISSLPSWKFK----RIDDSAS 286

Query: 286 PDNNNTGLPKEDPECCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQ 345
             ++++    +DPECCICLAKY DKEEVR+LPCSH FH KCVD+WL I S CPLCKQ L 
Sbjct: 287 DSDSDSATVTDDPECCICLAKYKDKEEVRKLPCSHKFHSKCVDQWLRIISCCPLCKQDLP 343

BLAST of Lcy12g009260 vs. TAIR 10
Match: AT4G26580.1 (RING/U-box superfamily protein )

HSP 1 Score: 411.8 bits (1057), Expect = 5.6e-115
Identity = 205/335 (61.19%), Postives = 250/335 (74.63%), Query Frame = 0

Query: 12  SFNSDATTSFISTPLSGEDGATADDIRNDTSDSLP-PSFWVRTAMKISRARWFIFLRRVF 71
           S    +++SF ST L        DD  +   +S P  SF++R AM++SRARWFIFLRRVF
Sbjct: 16  SITVSSSSSFASTQL--------DDNTHTAPESSPSSSFFMRLAMRVSRARWFIFLRRVF 75

Query: 72  HYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWPMRLWIGGYDL 131
           HYQN SRSDLG NPFNS +WM  EL+ALL Q+ +   TLA+S+ ERPVWPMRLWI GY++
Sbjct: 76  HYQNASRSDLGTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLWITGYNV 135

Query: 132 GCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSN--DSSRYSHLMNKCRTSLDLFFAIW 191
           GC+L+L+LLYGRY        +     DIEQQ+ +  +++R SHLMNKCRTSL+LFFAIW
Sbjct: 136 GCLLNLMLLYGRYRQQDTSHENAFSFGDIEQQQRSREETTRCSHLMNKCRTSLELFFAIW 195

Query: 192 FVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLIGY 251
           FV+GN+WVFDSRF SF  AP LH+LC  LL WNA+CYSFPF+LFLLLCC VPL+SS +GY
Sbjct: 196 FVIGNVWVFDSRFGSFHYAPILHVLCISLLAWNALCYSFPFLLFLLLCCVVPLVSSFLGY 255

Query: 252 NINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECCICLAKYID 311
           N+N+GS+EKGA DDQIS LP W+YK ++   +  SQ +N        DPECCICLAKY +
Sbjct: 256 NMNVGSSEKGASDDQISSLPSWKYKLID-ETSDSSQANN--------DPECCICLAKYKE 315

Query: 312 KEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQL 344
           KEEVR+LPCSH FHLKCVD+WL I S CPLCKQ L
Sbjct: 316 KEEVRKLPCSHRFHLKCVDQWLRIISCCPLCKQDL 333

BLAST of Lcy12g009260 vs. TAIR 10
Match: AT4G26580.2 (RING/U-box superfamily protein )

HSP 1 Score: 411.8 bits (1057), Expect = 5.6e-115
Identity = 205/335 (61.19%), Postives = 250/335 (74.63%), Query Frame = 0

Query: 12  SFNSDATTSFISTPLSGEDGATADDIRNDTSDSLP-PSFWVRTAMKISRARWFIFLRRVF 71
           S    +++SF ST L        DD  +   +S P  SF++R AM++SRARWFIFLRRVF
Sbjct: 16  SITVSSSSSFASTQL--------DDNTHTAPESSPSSSFFMRLAMRVSRARWFIFLRRVF 75

Query: 72  HYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWPMRLWIGGYDL 131
           HYQN SRSDLG NPFNS +WM  EL+ALL Q+ +   TLA+S+ ERPVWPMRLWI GY++
Sbjct: 76  HYQNASRSDLGTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLWITGYNV 135

Query: 132 GCVLSLLLLYGRYWYHYLIQGDGNGLLDIEQQRSN--DSSRYSHLMNKCRTSLDLFFAIW 191
           GC+L+L+LLYGRY        +     DIEQQ+ +  +++R SHLMNKCRTSL+LFFAIW
Sbjct: 136 GCLLNLMLLYGRYRQQDTSHENAFSFGDIEQQQRSREETTRCSHLMNKCRTSLELFFAIW 195

Query: 192 FVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLIGY 251
           FV+GN+WVFDSRF SF  AP LH+LC  LL WNA+CYSFPF+LFLLLCC VPL+SS +GY
Sbjct: 196 FVIGNVWVFDSRFGSFHYAPILHVLCISLLAWNALCYSFPFLLFLLLCCVVPLVSSFLGY 255

Query: 252 NINMGSTEKGALDDQISQLPCWRYKAVEANINPGSQPDNNNTGLPKEDPECCICLAKYID 311
           N+N+GS+EKGA DDQIS LP W+YK ++   +  SQ +N        DPECCICLAKY +
Sbjct: 256 NMNVGSSEKGASDDQISSLPSWKYKLID-ETSDSSQANN--------DPECCICLAKYKE 315

Query: 312 KEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQL 344
           KEEVR+LPCSH FHLKCVD+WL I S CPLCKQ L
Sbjct: 316 KEEVRKLPCSHRFHLKCVDQWLRIISCCPLCKQDL 333

BLAST of Lcy12g009260 vs. TAIR 10
Match: AT4G32600.1 (RING/U-box superfamily protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-49
Identity = 111/305 (36.39%), Postives = 165/305 (54.10%), Query Frame = 0

Query: 83  NPFNSGSWMAMELVALLFQLIISVLTLAISQTERPVWPMRLWIGGYDLGCVLSLLLLYGR 142
           +P NSG W+++EL   + Q+I +++ L++S+ E P  P+  WI GY  GCV +L LLY R
Sbjct: 105 SPLNSGLWISIELFLTVGQIIAAIVVLSLSKHEHPRAPLFTWIVGYACGCVATLPLLYWR 164

Query: 143 YWYHYLIQGDGNGL---------------------LDIEQQRSNDS--SRYSHLMNKCR- 202
           Y++        +G                       D  Q  +  S  SRY   ++  R 
Sbjct: 165 YYHSNQASEQDSGQHRPNLNVAAGPFAFSISRTSEADGRQTNTTSSRGSRYPGFISAARL 224

Query: 203 --------TSLDLFFAIWFVMGNLWVFDSRFASFQKAPKLHLLCSFLLVWNAICYSFPFI 262
                    +LD FFA+WFV+GN+W+F    +S  +AP L+ LC   L ++ I Y+ PFI
Sbjct: 225 KVIVEYFKMALDCFFAVWFVVGNVWIFGGH-SSAAEAPNLYRLCLVFLTFSCIGYAMPFI 284

Query: 263 LFLLLCCCVPLISSLIGYNINMGSTEKGALDDQISQLPCWRYKAVEANIN---PGSQPD- 322
           L   +CCC+P I S++GY  ++ +  +GA  + I+ LP  ++K  ++  N    GS    
Sbjct: 285 LCTTICCCLPCIISILGYREDL-TQPRGATPESINALPTHKFKLKKSRSNGDDNGSSTSE 344

Query: 323 --------NNNTGLPKEDPECCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPL 344
                   +N   +  ED  CCICLAKY + EE+R+LPCSH FH +CVDKWL I +SCPL
Sbjct: 345 GGVVAAGTDNERAISGEDAVCCICLAKYANNEELRELPCSHFFHKECVDKWLKINASCPL 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z921.9e-2730.15E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q8GUU29.5e-2729.85E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q8LDB81.8e-2527.06E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q9LN711.2e-2425.58E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q9FL073.3e-1139.51RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CI941.7e-18390.49E3 ubiquitin-protein ligase At4g11680 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0K4H05.9e-18189.34RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G278230 P... [more]
A0A6J1FHW35.9e-18188.41E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1CCU01.3e-18089.57E3 ubiquitin-protein ligase At1g63170 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1J2C94.2e-17987.25E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038899145.16.7e-18791.91E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida][more]
XP_008463025.13.4e-18390.49PREDICTED: E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis melo][more]
KAG7037312.19.3e-18188.41E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_011659087.11.2e-18089.34E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis sativus][more]
XP_022940226.11.2e-18088.41E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G55970.12.6e-12067.11RING/U-box superfamily protein [more]
AT5G55970.22.6e-12067.11RING/U-box superfamily protein [more]
AT4G26580.15.6e-11561.19RING/U-box superfamily protein [more]
AT4G26580.25.6e-11561.19RING/U-box superfamily protein [more]
AT4G32600.11.1e-4936.39RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 299..339
e-value: 6.4E-7
score: 39.0
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 298..340
e-value: 5.2E-13
score: 49.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 299..340
score: 12.563043
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 248..344
e-value: 1.3E-19
score: 71.6
NoneNo IPR availablePANTHERPTHR46225:SF1RING/U-BOX SUPERFAMILY PROTEINcoord: 32..344
NoneNo IPR availablePANTHERPTHR46225C3H4 TYPE ZINC FINGER PROTEINcoord: 32..344
NoneNo IPR availableCDDcd16474RING-H2_RNF111_likecoord: 297..340
e-value: 5.0407E-19
score: 77.3517
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 294..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g009260.1Lcy12g009260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008270 zinc ion binding