Lcy12g009060 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g009060
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	DUF2431 domain-containing protein
Location	Chr12: 35652936 .. 35654014 (+)
RNA-Seq Expression	Lcy12g009060
Synteny	Lcy12g009060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGACTTGAACAGTACAGAATTGTTCAGATCCGTGGCCCGCGAGATCAAGACGATAAAGCATTACAATAGCTCCCACAGAATACTCCTCGTCGGAGAAGGCGACTTCTCCTTCTCCGTTTGCTTGGCCGTAGCTTTCGGCTCGGCTGCTAACATGGTTGCCACTTCCCTCGACTCTAAAGGTATTTAAGTTTATCTACTCTCTCAAAAAAGTATTCAAAGGGACTAGAACAATATAATTATGATCATGGTTTTACTTGATAAAATATGTCAGATAGGAGAAAAAAATCAAGATTTTGTGTTATATCGTCAATCTGCTACAATTTTTTATTTTTATCTCGTATTTAGATCTCCCATCTTGTCACATTTCAATTCTCTCGCTCATTTAAATAACTTTCACCATATTGTAGCAGCCACATTAAATAATTTTTTTTTTGTTCGGTTGATGTTTTTGAATATGCAACGTGAAAGAGTCATTACTGAGAAAGTACATTCGTGTTGGCACGAACTTGAAATTCTTGGAGGAGCATGGGTGCACCGTGGTGCATGAAGAGGATGCGACAACAATGAGCCAACACCCACTTCTCCGCTACGAGTCATTCGATCGTATAGTCTTCAACTTTCCACACGCCGGGTTTCATTTTAAAGAAAGTGAAATCGAACAAATTACGTGAGTATACATACATGCATGGTTAATTTTGTTCTGACTCGGCCCCTGTTTGAGGTGGTTTATGAGTGGGGTTTTATCGGTTTTTTGACAGACTCCACCGGGATCTAGTGAGAGGGTTTCTAAGGAATGCAAAGGAAATGGTTGGTGAAAATGGAGAAATTCACATCACCCACAAGACTACACATCCTTTCAATGAATGGAAGATTGTGGAATTGGCTCAAGAAGTGGGGCTGTTTCTCAAAGAGAGAGCTTATTTTGTGATGGGGGAATATCCATACTATCGGAATAAGAGAGGAGATGGATGGAACAGCGACGACACTTTCCCCGTTGGAAAATGTTGTACCTACAAATTTGCAAGAAATAATAATCCATCTTTCCTTAATTATGATCACCACTATGTGATCTAG

mRNA sequence

ATGGGAGACTTGAACAGTACAGAATTGTTCAGATCCGTGGCCCGCGAGATCAAGACGATAAAGCATTACAATAGCTCCCACAGAATACTCCTCGTCGGAGAAGGCGACTTCTCCTTCTCCGTTTGCTTGGCCGTAGCTTTCGGCTCGGCTGCTAACATGGTTGCCACTTCCCTCGACTCTAAAGAGTCATTACTGAGAAAGTACATTCGTGTTGGCACGAACTTGAAATTCTTGGAGGAGCATGGGTGCACCGTGGTGCATGAAGAGGATGCGACAACAATGAGCCAACACCCACTTCTCCGCTACGAGTCATTCGATCGTATAGTCTTCAACTTTCCACACGCCGGGTTTCATTTTAAAGAAAGTGAAATCGAACAAATTACACTCCACCGGGATCTAGTGAGAGGGTTTCTAAGGAATGCAAAGGAAATGGTTGGTGAAAATGGAGAAATTCACATCACCCACAAGACTACACATCCTTTCAATGAATGGAAGATTGTGGAATTGGCTCAAGAAGTGGGGCTGTTTCTCAAAGAGAGAGCTTATTTTGTGATGGGGGAATATCCATACTATCGGAATAAGAGAGGAGATGGATGGAACAGCGACGACACTTTCCCCGTTGGAAAATGTTGTACCTACAAATTTGCAAGAAATAATAATCCATCTTTCCTTAATTATGATCACCACTATGTGATCTAG

Coding sequence (CDS)

ATGGGAGACTTGAACAGTACAGAATTGTTCAGATCCGTGGCCCGCGAGATCAAGACGATAAAGCATTACAATAGCTCCCACAGAATACTCCTCGTCGGAGAAGGCGACTTCTCCTTCTCCGTTTGCTTGGCCGTAGCTTTCGGCTCGGCTGCTAACATGGTTGCCACTTCCCTCGACTCTAAAGAGTCATTACTGAGAAAGTACATTCGTGTTGGCACGAACTTGAAATTCTTGGAGGAGCATGGGTGCACCGTGGTGCATGAAGAGGATGCGACAACAATGAGCCAACACCCACTTCTCCGCTACGAGTCATTCGATCGTATAGTCTTCAACTTTCCACACGCCGGGTTTCATTTTAAAGAAAGTGAAATCGAACAAATTACACTCCACCGGGATCTAGTGAGAGGGTTTCTAAGGAATGCAAAGGAAATGGTTGGTGAAAATGGAGAAATTCACATCACCCACAAGACTACACATCCTTTCAATGAATGGAAGATTGTGGAATTGGCTCAAGAAGTGGGGCTGTTTCTCAAAGAGAGAGCTTATTTTGTGATGGGGGAATATCCATACTATCGGAATAAGAGAGGAGATGGATGGAACAGCGACGACACTTTCCCCGTTGGAAAATGTTGTACCTACAAATTTGCAAGAAATAATAATCCATCTTTCCTTAATTATGATCACCACTATGTGATCTAG

Protein sequence

MGDLNSTELFRSVAREIKTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKFLEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGDGWNSDDTFPVGKCCTYKFARNNNPSFLNYDHHYVI

Homology

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BLAST of Lcy12g009060 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.2e-56
Identity = 110/204 (53.92%), Postives = 143/204 (70.10%), Query Frame = 0


Query: 18  KTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKF 77
           K I+HY+S+H+ILLVGEG+FSFS+CLA AFGSA N+ ATSLDS++ L  KY+    N+  
Sbjct: 6   KWIRHYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKYMDAVDNINI 65

Query: 78  LEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHF--KESEIEQITLHRDLVR 137
           L+ +GC + HE D  TMS    L  + +DRIVFNFPHAG  F  +E     I  H++LVR
Sbjct: 66  LKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESHKELVR 125

Query: 138 GFLRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKR 197
           GFL NAKEM+ E+GEIHITHKTT+PF++W I +L +  GL L +++ F +  YP Y  KR
Sbjct: 126 GFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPGYITKR 185

Query: 198 GDGW-NSDDTFPVGKCCTYKFARN 219
           G G   SDD FPVG+C TY F ++
Sbjct: 186 GSGGRRSDDYFPVGECSTYMFTQS 209

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BLAST of Lcy12g009060 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.2e-55
Identity = 102/196 (52.04%), Postives = 134/196 (68.37%), Query Frame = 0


Query: 20  IKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKFLE 79
           +KHY+S+H+ILLVGEGDFSFS  LA  FGSA+N+ A+SLDS + ++RKY +  +NLK L+
Sbjct: 21  VKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKKARSNLKTLK 80

Query: 80  EHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLR 139
             G  ++H  DATT+  HP LRY  FDR++FNFPHAGFH +ES+   I  HR+LV GF  
Sbjct: 81  RLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGFFN 140

Query: 140 NAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGDGW 199
            A  ++  NGE+H++HK   PF+EW + ELA    L L +R  F    YP Y NKRGDG 
Sbjct: 141 GASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGDGR 200

Query: 200 NSDDTFPVGKCCTYKF 216
             D  F +G+C T+KF
Sbjct: 201 RCDQPFLLGECSTFKF 216

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BLAST of Lcy12g009060 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.6e-22
Identity = 62/178 (34.83%), Postives = 96/178 (53.93%), Query Frame = 0


Query: 23  YNSSHRILLVGEGDFSFSVCLAVAFGSAANMV-ATSLDSKESLLRKYIRVGTNLKFLEEH 82
           +  ++R LL+GEG+FSF+  L +   S+   V ATS DSKE L +KY      +  +E +
Sbjct: 54  FEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLATSYDSKEDLKQKYPDAAEYISKIEIN 113

Query: 83  GCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLRNA 142
           G  V+HE DAT +  H  L+ + FD I +NFPH+G   K+ +   I  ++ ++  F + +
Sbjct: 114 GGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHSGKGIKDQD-RNILDNQKMLLAFFKAS 173

Query: 143 KEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGDGW 200
           K ++ E G I IT   T P+  W +  LA++ G        F    YP Y ++R  GW
Sbjct: 174 KFLLSEKGVIVITLAETKPYTLWNLKGLAKDAGYTSLMTEKFDSSFYPEYSHRRTIGW 230

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BLAST of Lcy12g009060 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 8.9e-13
Identity = 58/205 (28.29%), Postives = 91/205 (44.39%), Query Frame = 0


Query: 28  RILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKFLEEHGCTVVH 87
           R+LLVGEG+FSF+  L      + ++ AT    + +L    + +  NLK L E G  V  
Sbjct: 5   RLLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAALEGDPVAL-ENLKRLRERGVEVRF 64

Query: 88  EEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLRNAKEMVGE 147
             D T +S         FDRI FNFPH G          +  +R+L+  F ++  +++ +
Sbjct: 65  GVDCTQLSHALPADDRDFDRIYFNFPHCG------RKAGVAKNRELLAKFFQSCADVLAK 124

Query: 148 NGEIHIT-------------HKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNK 207
            GE+H+T              +  H  N W++V +A   G  L +   F     P Y   
Sbjct: 125 AGEVHVTLCRGQGGTPADKPQREWH--NSWQVVAMAALGGFILSDVCPFSCEAVPGY--- 184

Query: 208 RGDGWNSDD-TFPVGKCCTYKFARN 219
           +  G+ S D  F +    TY F ++
Sbjct: 185 KCTGYRSQDRPFHIEGALTYIFTQS 197

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BLAST of Lcy12g009060 vs. ExPASy Swiss-Prot
Match: Q9BRP7 (Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=FDXACB1 PE=1 SV=3)

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-11
Identity = 48/165 (29.09%), Postives = 75/165 (45.45%), Query Frame = 0


Query: 28  RILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKFLEEHGCTVVH 87
           R+LLVGEG+FSF+  L+     +  + AT L     L R  +    NL+ L E G  V  
Sbjct: 5   RLLLVGEGNFSFAAALSETLDQSTQLTATCLQRPAELARDPL-AWENLQCLRERGIDVRF 64

Query: 88  EEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLRNAKEMVGE 147
             D T ++    L    FD+I F FPH G          +  +R+L+  F ++  +++ E
Sbjct: 65  GVDCTQLADVFELHEREFDQIYFIFPHCG------RKAGVAKNRELLAKFFQSCADVLAE 124

Query: 148 NGEIHIT-------------HKTTHPFNEWKIVELAQEVGLFLKE 180
            GE+H+               +  H  N W++V +A   GL L +
Sbjct: 125 EGEVHVALCRGQGGTPADKPQREWH--NSWQVVAMAALGGLILSD 160

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BLAST of Lcy12g009060 vs. ExPASy TrEMBL
Match: A0A6J1CDH1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010537 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.1e-85
Identity = 159/216 (73.61%), Postives = 175/216 (81.02%), Query Frame = 0


Query: 1   MGDLNSTELFRSVAREIKTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDS 60
           M DLN+       +R+ K IKHY+SSH ILLVGEGDFSFS CLA  FGSAANMVATSLDS
Sbjct: 1   MEDLNN-------SRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDS 60

Query: 61  KESLLRKYIRVGTNLKFLEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFK 120
           KESLL KY  V TNLK LEE GCTV H+ DAT+MSQHPLLR++SFDR+VFNFPHAGFHFK
Sbjct: 61  KESLLSKYTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFK 120

Query: 121 ESEIEQITLHRDLVRGFLRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKER 180
           ES+I QI LHR LVRGFLRNAKE +GE GEIHITHKT HPF+ W+IVELA+E GL LKE 
Sbjct: 121 ESDIRQIELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEE 180

Query: 181 AYFVMGEYPYYRNKRGDGWNSDDTFPVGKCCTYKFA 217
             F + EYP Y NKRGDGWNSD TFPVG C T+KFA
Sbjct: 181 PNFFLWEYPNYENKRGDGWNSDGTFPVGACRTFKFA 209

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BLAST of Lcy12g009060 vs. ExPASy TrEMBL
Match: A0A5D3DIB6 (DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G002400 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.6e-71
Identity = 134/198 (67.68%), Postives = 155/198 (78.28%), Query Frame = 0


Query: 18  KTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKF 77
           K+IKHY+SSH+ILLVGEGDFSFS CLA AF SA+N+VATSLDS + L+ KY     NLK 
Sbjct: 7   KSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHANRNLKI 66

Query: 78  LEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGF 137
           LEE G  V+H+ DATTMSQHPLL    FDRIVFNFPHAGF + E    QI LHRDLVRGF
Sbjct: 67  LEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRDLVRGF 126

Query: 138 LRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGD 197
           LRNAK M+ ++GEIHITHKT+HPF++W+IV+LA   GL LKE A F   +YP Y NKRGD
Sbjct: 127 LRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYENKRGD 186

Query: 198 GWNSDDTFPVGKCCTYKF 216
           GWNS+ TFPVG C T+KF
Sbjct: 187 GWNSNGTFPVGACSTFKF 204

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BLAST of Lcy12g009060 vs. ExPASy TrEMBL
Match: A0A1S3BIC4 (uncharacterized protein At4g26485-like OS=Cucumis melo OX=3656 GN=LOC103490386 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.6e-71
Identity = 134/198 (67.68%), Postives = 155/198 (78.28%), Query Frame = 0


Query: 18  KTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKF 77
           K+IKHY+SSH+ILLVGEGDFSFS CLA AF SA+N+VATSLDS + L+ KY     NLK 
Sbjct: 7   KSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHANRNLKI 66

Query: 78  LEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGF 137
           LEE G  V+H+ DATTMSQHPLL    FDRIVFNFPHAGF + E    QI LHRDLVRGF
Sbjct: 67  LEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRDLVRGF 126

Query: 138 LRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGD 197
           LRNAK M+ ++GEIHITHKT+HPF++W+IV+LA   GL LKE A F   +YP Y NKRGD
Sbjct: 127 LRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYENKRGD 186

Query: 198 GWNSDDTFPVGKCCTYKF 216
           GWNS+ TFPVG C T+KF
Sbjct: 187 GWNSNGTFPVGACSTFKF 204

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BLAST of Lcy12g009060 vs. ExPASy TrEMBL
Match: A0A6J1CDR1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010540 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.4e-69
Identity = 134/197 (68.02%), Postives = 158/197 (80.20%), Query Frame = 0


Query: 22  HYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKY-IRVGTNLKFLEE 81
           HYNSSH+ILLVGEG+FSFSVCLA A GS  N+VATSLDSK++LLRKY   + T L+ LEE
Sbjct: 2   HYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELEE 61

Query: 82  HGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLRN 141
            GC V+H  +  TMSQHPLLR E FDRIVFNFPHAGF + E E +QI LH++LVR F+RN
Sbjct: 62  EGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRN 121

Query: 142 AKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGDGWN 201
           A EMV ENGEIHITHKT+HPF+EW+IVE+A+E GLFLKE A F   +YP Y NK+GDG  
Sbjct: 122 AVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGPY 181

Query: 202 SDDTFPVGKCCTYKFAR 218
            + TFPVG CCT+KFA+
Sbjct: 182 CNRTFPVGCCCTFKFAK 198

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BLAST of Lcy12g009060 vs. ExPASy TrEMBL
Match: A0A0A0K734 (DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.2e-68
Identity = 135/213 (63.38%), Postives = 156/213 (73.24%), Query Frame = 0


Query: 3   DLNSTELFRSVAREIKTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKE 62
           D+N+TE         K+I HY+SSH ILLVGEGDFSFS CLA AF SA+N++ATSLDS++
Sbjct: 2   DINNTE---------KSIMHYSSSHNILLVGEGDFSFSACLATAFASASNIIATSLDSRD 61

Query: 63  SLLRKYIRVGTNLKFLEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKES 122
            L+ KY R   N+K LEE G TV+HE DATTMSQHPLL    FDRIVFNFPHAGF +KES
Sbjct: 62  ELVMKYARAARNVKILEELGGTVLHEVDATTMSQHPLLHDMLFDRIVFNFPHAGFVYKES 121

Query: 123 EIEQITLHRDLVRGFLRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAY 182
              QI LHR+LVR FLRNAK M+   GEIHITHKT+HPF++W IV LA + GL LKE   
Sbjct: 122 NTAQIELHRNLVRVFLRNAKSMIDVEGEIHITHKTSHPFSKWGIVNLACDEGLCLKESEI 181

Query: 183 FVMGEYPYYRNKRGDGWNSDDTFPVGKCCTYKF 216
           F   +YP Y NKRG G N D TFPVG C T+KF
Sbjct: 182 FHAWQYPNYENKRGHGQNPDGTFPVGACSTFKF 205

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BLAST of Lcy12g009060 vs. NCBI nr
Match: XP_022139691.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 326.2 bits (835), Expect = 2.2e-85
Identity = 159/216 (73.61%), Postives = 175/216 (81.02%), Query Frame = 0


Query: 1   MGDLNSTELFRSVAREIKTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDS 60
           M DLN+       +R+ K IKHY+SSH ILLVGEGDFSFS CLA  FGSAANMVATSLDS
Sbjct: 1   MEDLNN-------SRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDS 60

Query: 61  KESLLRKYIRVGTNLKFLEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFK 120
           KESLL KY  V TNLK LEE GCTV H+ DAT+MSQHPLLR++SFDR+VFNFPHAGFHFK
Sbjct: 61  KESLLSKYTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFK 120

Query: 121 ESEIEQITLHRDLVRGFLRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKER 180
           ES+I QI LHR LVRGFLRNAKE +GE GEIHITHKT HPF+ W+IVELA+E GL LKE 
Sbjct: 121 ESDIRQIELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEE 180

Query: 181 AYFVMGEYPYYRNKRGDGWNSDDTFPVGKCCTYKFA 217
             F + EYP Y NKRGDGWNSD TFPVG C T+KFA
Sbjct: 181 PNFFLWEYPNYENKRGDGWNSDGTFPVGACRTFKFA 209

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BLAST of Lcy12g009060 vs. NCBI nr
Match: XP_008448100.1 (PREDICTED: uncharacterized protein At4g26485-like [Cucumis melo])

HSP 1 Score: 278.5 bits (711), Expect = 5.3e-71
Identity = 134/198 (67.68%), Postives = 155/198 (78.28%), Query Frame = 0


Query: 18  KTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKF 77
           K+IKHY+SSH+ILLVGEGDFSFS CLA AF SA+N+VATSLDS + L+ KY     NLK 
Sbjct: 7   KSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHANRNLKI 66

Query: 78  LEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGF 137
           LEE G  V+H+ DATTMSQHPLL    FDRIVFNFPHAGF + E    QI LHRDLVRGF
Sbjct: 67  LEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRDLVRGF 126

Query: 138 LRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGD 197
           LRNAK M+ ++GEIHITHKT+HPF++W+IV+LA   GL LKE A F   +YP Y NKRGD
Sbjct: 127 LRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYENKRGD 186

Query: 198 GWNSDDTFPVGKCCTYKF 216
           GWNS+ TFPVG C T+KF
Sbjct: 187 GWNSNGTFPVGACSTFKF 204

[Close]

BLAST of Lcy12g009060 vs. NCBI nr
Match: TYK23208.1 (uncharacterized protein E5676_scaffold142G002400 [Cucumis melo var. makuwa])

HSP 1 Score: 278.5 bits (711), Expect = 5.3e-71
Identity = 134/198 (67.68%), Postives = 155/198 (78.28%), Query Frame = 0


Query: 18  KTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKF 77
           K+IKHY+SSH+ILLVGEGDFSFS CLA AF SA+N+VATSLDS + L+ KY     NLK 
Sbjct: 7   KSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHANRNLKI 66

Query: 78  LEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGF 137
           LEE G  V+H+ DATTMSQHPLL    FDRIVFNFPHAGF + E    QI LHRDLVRGF
Sbjct: 67  LEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRDLVRGF 126

Query: 138 LRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGD 197
           LRNAK M+ ++GEIHITHKT+HPF++W+IV+LA   GL LKE A F   +YP Y NKRGD
Sbjct: 127 LRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYENKRGD 186

Query: 198 GWNSDDTFPVGKCCTYKF 216
           GWNS+ TFPVG C T+KF
Sbjct: 187 GWNSNGTFPVGACSTFKF 204

[Close]

BLAST of Lcy12g009060 vs. NCBI nr
Match: KAG7032604.1 (hypothetical protein SDJN02_06653, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.9 bits (699), Expect = 1.3e-69
Identity = 134/207 (64.73%), Postives = 157/207 (75.85%), Query Frame = 0


Query: 18  KTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKF 77
           KTI HY SSH ILLVGEGDFSFS CLA AFGSA NMVATSLDS E LLRKY RV  NL+ 
Sbjct: 17  KTIMHYKSSHTILLVGEGDFSFSACLAKAFGSATNMVATSLDSNEVLLRKYSRVAANLEA 76

Query: 78  LEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGF 137
           L   G TV+HE DAT MS+H  L Y+ FDRIVFNFPHAGF F+E++  QI LH+DLVRGF
Sbjct: 77  LGLLGGTVLHEVDATAMSRHCSLCYKEFDRIVFNFPHAGFSFRETDAIQIKLHQDLVRGF 136

Query: 138 LRNAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGD 197
           LR A+++V + GEIHITHK ++P+ EW+I ELA++ GL LKE A F + +YP Y NKRG+
Sbjct: 137 LREARKLVSDKGEIHITHKISYPYCEWEIEELAKKEGLLLKETAEFSLWDYPNYENKRGE 196

Query: 198 GWNSDDTFPVGKCCTYKFARNNNPSFL 225
           G NSD TFPVG C T+KF R     F+
Sbjct: 197 GGNSDHTFPVGACATFKFVRGTKVKFI 223

[Close]

BLAST of Lcy12g009060 vs. NCBI nr
Match: XP_022139694.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 272.7 bits (696), Expect = 2.9e-69
Identity = 134/197 (68.02%), Postives = 158/197 (80.20%), Query Frame = 0


Query: 22  HYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKY-IRVGTNLKFLEE 81
           HYNSSH+ILLVGEG+FSFSVCLA A GS  N+VATSLDSK++LLRKY   + T L+ LEE
Sbjct: 2   HYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELEE 61

Query: 82  HGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLRN 141
            GC V+H  +  TMSQHPLLR E FDRIVFNFPHAGF + E E +QI LH++LVR F+RN
Sbjct: 62  EGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRN 121

Query: 142 AKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGDGWN 201
           A EMV ENGEIHITHKT+HPF+EW+IVE+A+E GLFLKE A F   +YP Y NK+GDG  
Sbjct: 122 AVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGPY 181

Query: 202 SDDTFPVGKCCTYKFAR 218
            + TFPVG CCT+KFA+
Sbjct: 182 CNRTFPVGCCCTFKFAK 198

[Close]

BLAST of Lcy12g009060 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 216.5 bits (550), Expect = 2.3e-56
Identity = 102/196 (52.04%), Postives = 134/196 (68.37%), Query Frame = 0


Query: 20  IKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKFLE 79
           +KHY+S+H+ILLVGEGDFSFS  LA  FGSA+N+ A+SLDS + ++RKY +  +NLK L+
Sbjct: 21  VKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKKARSNLKTLK 80

Query: 80  EHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLVRGFLR 139
             G  ++H  DATT+  HP LRY  FDR++FNFPHAGFH +ES+   I  HR+LV GF  
Sbjct: 81  RLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGFFN 140

Query: 140 NAKEMVGENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGDGW 199
            A  ++  NGE+H++HK   PF+EW + ELA    L L +R  F    YP Y NKRGDG 
Sbjct: 141 GASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGDGR 200

Query: 200 NSDDTFPVGKCCTYKF 216
             D  F +G+C T+KF
Sbjct: 201 RCDQPFLLGECSTFKF 216

[Close]

BLAST of Lcy12g009060 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 170.6 bits (431), Expect = 1.5e-42
Identity = 95/188 (50.53%), Postives = 122/188 (64.89%), Query Frame = 0


Query: 20  IKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTNLKFLE 79
           ++ Y++  +ILLVGEGDFSFS+ LA  FGSA N+ ATSLD++E L  KY     N++ LE
Sbjct: 9   LQQYSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKYTDGKANVEGLE 68

Query: 80  EHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHF-KESEIEQITLHRDLVRGFL 139
             GCTVVH  +  +MS     R   +DRI+FNFPH+G  F  E +I  I LH+ LVRGFL
Sbjct: 69  LFGCTVVHGVNVHSMSSD--YRLGRYDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGFL 128

Query: 140 RNAKEMV-GENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRNKRGD 199
            +A++M+  E+GEIH+THKTT PFN W I  LA E GL L     F    +P Y NK+G 
Sbjct: 129 ESARKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKGG 188

Query: 200 GWNSDDTF 206
           G N + TF
Sbjct: 189 GSNCNSTF 194

[Close]

BLAST of Lcy12g009060 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 169.9 bits (429), Expect = 2.5e-42
Identity = 85/170 (50.00%), Postives = 112/170 (65.88%), Query Frame = 0


Query: 52  NMVATSLDSKESLLRKYIRVGTNLKFLEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFN 111
           N+ ATSLDS++ L  KY+    N+  L+ +GC + HE D  TMS    L  + +DRIVFN
Sbjct: 2   NITATSLDSEDELSIKYMDAVDNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFN 61

Query: 112 FPHAGFHF--KESEIEQITLHRDLVRGFLRNAKEMVGENGEIHITHKTTHPFNEWKIVEL 171
           FPHAG  F  +E     I  H++LVRGFL NAKEM+ E+GEIHITHKTT+PF++W I +L
Sbjct: 62  FPHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKL 121

Query: 172 AQEVGLFLKERAYFVMGEYPYYRNKRGDGW-NSDDTFPVGKCCTYKFARN 219
            +  GL L +++ F +  YP Y  KRG G   SDD FPVG+C TY F ++
Sbjct: 122 GKGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPVGECSTYMFTQS 171

[Close]

BLAST of Lcy12g009060 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 165.2 bits (417), Expect = 6.1e-41
Identity = 87/207 (42.03%), Postives = 127/207 (61.35%), Query Frame = 0


Query: 15  REIKTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTN 74
           +E K ++HY +  +ILLVGEGDFSFS+ LA AFGSA+N+ ATSLD++  L +K+     N
Sbjct: 62  QETKRLRHYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKFKNGKAN 121

Query: 75  LKFLEEHGCTVVHEEDATTMSQHPLLRYES-FDRIVFNFPHAGFHFKESEIEQITLHRDL 134
           ++ LE  GC+VV+  +  +M   P +   + +DR++FNFP                  +L
Sbjct: 122 VEELERLGCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFP----------------THEL 181

Query: 135 VRGFLRNAKEMV---GENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPY 194
           VRGF+++A+ +V    + GEIH+ HKT +PF+EWK+  L ++ GL L     F +  YP 
Sbjct: 182 VRGFMKSARVLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFCLSHYPG 241

Query: 195 YRNKRGDGWNSDDTFPVGKCCTYKFAR 218
           Y NKRG G  SD +FPVGK  T+ F +
Sbjct: 242 YFNKRGSGGYSDSSFPVGKSSTFMFTK 252

[Close]

BLAST of Lcy12g009060 vs. TAIR 10
Match: AT5G25030.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 150.2 bits (378), Expect = 2.0e-36
Identity = 85/204 (41.67%), Postives = 118/204 (57.84%), Query Frame = 0


Query: 15  REIKTIKHYNSSHRILLVGEGDFSFSVCLAVAFGSAANMVATSLDSKESLLRKYIRVGTN 74
           +E K +  Y++  +IL+VGEG+FSFS+ LA A GSA N+ A SLD +E L R Y     N
Sbjct: 4   QESKRLSRYSNEQKILVVGEGEFSFSLSLAKALGSATNITAISLDIREDLGRNYNNGKGN 63

Query: 75  LKFLEEHGCTVVHEEDATTMSQHPLLRYESFDRIVFNFPHAGFHFKESEIEQITLHRDLV 134
           ++ LE  GCTVV   +  +M     L +  +D I+FNFPHAG   K            + 
Sbjct: 64  VEELERLGCTVVRGVNVHSMKSDDRLAH--YDIIIFNFPHAGKRNK------------VF 123

Query: 135 RGFLRNAKEMV-GENGEIHITHKTTHPFNEWKIVELAQEVGLFLKERAYFVMGEYPYYRN 194
            GF+ +A+EM+  E+GEIHIT  T +PFN+W +  LA+E GL L +R  F+   +P   N
Sbjct: 124 GGFMESAREMMKDEDGEIHITLNTLNPFNKWDLKALAEESGLRLIQRMQFIKWAFPSSSN 183

Query: 195 KRGDGWNSDDTFPVGKCCTYKFAR 218
           KR  G N D  +P+G   TY F +
Sbjct: 184 KRESGSNCDFIYPIGSAITYMFKK 193

The following BLAST results are available for this feature:

BLAST of Lcy12g009060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C8L4	2.2e-56	53.92	Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 P...	[more]
F4I1X0	3.2e-55	52.04	Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX...	[more]
O94480	3.6e-22	34.83	25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 9...	[more]
Q3UY23	8.9e-13	28.29	Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus mus...	[more]
Q9BRP7	2.9e-11	29.09	Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX...	[more]

BLAST of Lcy12g009060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDH1	1.1e-85	73.61	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5D3DIB6	2.6e-71	67.68	DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BIC4	2.6e-71	67.68	uncharacterized protein At4g26485-like OS=Cucumis melo OX=3656 GN=LOC103490386 P...	[more]
A0A6J1CDR1	1.4e-69	68.02	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0K734	1.2e-68	63.38	DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=...	[more]

BLAST of Lcy12g009060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139691.1	2.2e-85	73.61	uncharacterized protein At4g26485-like [Momordica charantia]	[more]
XP_008448100.1	5.3e-71	67.68	PREDICTED: uncharacterized protein At4g26485-like [Cucumis melo]	[more]
TYK23208.1	5.3e-71	67.68	uncharacterized protein E5676_scaffold142G002400 [Cucumis melo var. makuwa]	[more]
KAG7032604.1	1.3e-69	64.73	hypothetical protein SDJN02_06653, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022139694.1	2.9e-69	68.02	uncharacterized protein At4g26485-like [Momordica charantia]	[more]

BLAST of Lcy12g009060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55790.1	2.3e-56	52.04	Domain of unknown function (DUF2431)	[more]
AT5G56060.1	1.5e-42	50.53	Domain of unknown function (DUF2431)	[more]
AT4G26485.1	2.5e-42	50.00	Domain of unknown function (DUF2431)	[more]
AT5G56075.1	6.1e-41	42.03	Domain of unknown function (DUF2431)	[more]
AT5G25030.1	2.0e-36	41.67	Domain of unknown function (DUF2431)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019446	Domain of unknown function DUF2431	PFAM	PF10354	DUF2431	coord: 30..195 e-value: 1.2E-46 score: 159.0
None	No IPR available	PANTHER	PTHR11538:SF66	DUF2431 DOMAIN PROTEIN	coord: 16..217
None	No IPR available	PANTHER	PTHR11538	PHENYLALANYL-TRNA SYNTHETASE	coord: 16..217
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 78..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g009060.1	Lcy12g009060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016020	membrane
molecular_function	GO:0070042	rRNA (uridine-N3-)-methyltransferase activity