Lcy12g008640 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g008640
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr12: 34843868 .. 34844686 (+)
RNA-Seq Expression	Lcy12g008640
Synteny	Lcy12g008640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATATTAACCCGTTGTGTTTTATGTGCAGGAAAAAGAGAGAAACAACTGAGCATTTATTTTGGGAGAGTAAGATTACCAAAGGTATATGGGACAAATTTTATCCTTCCTCTGACTTAACTTGTTTGGATGACAGGAAAGGTTGGGGAGCAAAGGATTACTGTGAGTTCCTGTGGAGAAGGTGTGATGGAGGCAGGATAGAAGAGAAGAGTTTGAATAAAAGTCTTATAATATGTTGGCAGATTTGGAAGCATACGAACGATGTGTACCATAAAAAGCAGCAGCCCAACCAAGAGGCGATGAAACATCAAATAGAGAAATACCTTGAGGAGTTCAAATGTGGGGACGAGGAGATTATGTCGGTCACGAGATGGATCCTGCCGCCGGAAGAAATTTGCAAGCTCAATTGTGACGCAACTTGGAACGATGAACGGAAACAAGGCGGCATCGGTTGGATCCTTCGGCATTGGAATGGATCCCCGTTGTCGGCAGGTTTCAGATGCATCAAGAGACAATGGAAGGTGCAATGGCTGGAGACATTGGCGATCAGTGACGGATTAAGAGCGCTGGAGATAATGTCTGGAGACATTCACGATCTGGCGCTTCAGGTGAGGGTGGAAACGGATGCTTTGCAAACCGTGAAACTCTTACTCTCCCAGGATCACGATGAGACAGATTGGGTCCACTTCATTCAAGAAGCAAAAGCCCTAATGGAAAGATGCAATATTGAAGCAGAAGTTCACATTAATAGATCTCACAATCAGTTGGCCCACCAGTTAGCCCAAAAAGCTAGTCTAGGTGGGGAGTCAGGGAGTTAG

mRNA sequence

ATGGATATTAACCCGTTGTGTTTTATGTGCAGGAAAAAGAGAGAAACAACTGAGCATTTATTTTGGGAGAGTAAGATTACCAAAGGTATATGGGACAAATTTTATCCTTCCTCTGACTTAACTTGTTTGGATGACAGGAAAGGTTGGGGAGCAAAGGATTACTGTGAGTTCCTGTGGAGAAGGTGTGATGGAGGCAGGATAGAAGAGAAGAGTTTGAATAAAAGTCTTATAATATGTTGGCAGATTTGGAAGCATACGAACGATGTGTACCATAAAAAGCAGCAGCCCAACCAAGAGGCGATGAAACATCAAATAGAGAAATACCTTGAGGAGTTCAAATGTGGGGACGAGGAGATTATGTCGGTCACGAGATGGATCCTGCCGCCGGAAGAAATTTGCAAGCTCAATTGTGACGCAACTTGGAACGATGAACGGAAACAAGGCGGCATCGGTTGGATCCTTCGGCATTGGAATGGATCCCCGTTGTCGGCAGGTTTCAGATGCATCAAGAGACAATGGAAGGTGCAATGGCTGGAGACATTGGCGATCAGTGACGGATTAAGAGCGCTGGAGATAATGTCTGGAGACATTCACGATCTGGCGCTTCAGGTGAGGGTGGAAACGGATGCTTTGCAAACCGTGAAACTCTTACTCTCCCAGGATCACGATGAGACAGATTGGGTCCACTTCATTCAAGAAGCAAAAGCCCTAATGGAAAGATGCAATATTGAAGCAGAAGTTCACATTAATAGATCTCACAATCAGTTGGCCCACCAGTTAGCCCAAAAAGCTAGTCTAGGTGGGGAGTCAGGGAGTTAG

Coding sequence (CDS)

ATGGATATTAACCCGTTGTGTTTTATGTGCAGGAAAAAGAGAGAAACAACTGAGCATTTATTTTGGGAGAGTAAGATTACCAAAGGTATATGGGACAAATTTTATCCTTCCTCTGACTTAACTTGTTTGGATGACAGGAAAGGTTGGGGAGCAAAGGATTACTGTGAGTTCCTGTGGAGAAGGTGTGATGGAGGCAGGATAGAAGAGAAGAGTTTGAATAAAAGTCTTATAATATGTTGGCAGATTTGGAAGCATACGAACGATGTGTACCATAAAAAGCAGCAGCCCAACCAAGAGGCGATGAAACATCAAATAGAGAAATACCTTGAGGAGTTCAAATGTGGGGACGAGGAGATTATGTCGGTCACGAGATGGATCCTGCCGCCGGAAGAAATTTGCAAGCTCAATTGTGACGCAACTTGGAACGATGAACGGAAACAAGGCGGCATCGGTTGGATCCTTCGGCATTGGAATGGATCCCCGTTGTCGGCAGGTTTCAGATGCATCAAGAGACAATGGAAGGTGCAATGGCTGGAGACATTGGCGATCAGTGACGGATTAAGAGCGCTGGAGATAATGTCTGGAGACATTCACGATCTGGCGCTTCAGGTGAGGGTGGAAACGGATGCTTTGCAAACCGTGAAACTCTTACTCTCCCAGGATCACGATGAGACAGATTGGGTCCACTTCATTCAAGAAGCAAAAGCCCTAATGGAAAGATGCAATATTGAAGCAGAAGTTCACATTAATAGATCTCACAATCAGTTGGCCCACCAGTTAGCCCAAAAAGCTAGTCTAGGTGGGGAGTCAGGGAGTTAG

Protein sequence

MDINPLCFMCRKKRETTEHLFWESKITKGIWDKFYPSSDLTCLDDRKGWGAKDYCEFLWRRCDGGRIEEKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIMSVTRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKRQWKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETDWVHFIQEAKALMERCNIEAEVHINRSHNQLAHQLAQKASLGGESGS

Homology

[Close]

BLAST of Lcy12g008640 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-20
Identity = 66/222 (29.73%), Postives = 99/222 (44.59%), Query Frame = 0


Query: 11  RKKRETTEHLFWESKITKGIWDKFYPSSDLTCLDDRKGWGAKDYCEFLWRRCDGGRIEEK 70
           RKK ETT+H+ WE K+ K IW    P        DR  W  K+Y E+L  +       E+
Sbjct: 131 RKKEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWEWLMDKAG-----EE 190

Query: 71  SLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIM---------- 130
              +S+II  QIW+  N    K        ++  I++Y+      D  +           
Sbjct: 191 ERRRSMIIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSAGQDTNLKRKSKDFHPIR 250

Query: 131 -----SVTRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKRQWKV 190
                +  RW  P     KLN DA W  +    GIGWILR   G  +  G R I+ +  +
Sbjct: 251 RIGDNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNI 310

Query: 191 QWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLL 218
            +LE +AI +GLRA+        +    + +E+D+L+ + LL
Sbjct: 311 TYLEVMAICEGLRAIR------QEHCRPIHLESDSLEAIHLL 341

[Close]

BLAST of Lcy12g008640 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-20
Identity = 71/215 (33.02%), Postives = 108/215 (50.23%), Query Frame = 0


Query: 66  RIEEKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIMSV--- 125
           +  ++ L+  LI  W IW H N V  + +  +   M  Q+ K++ E     E  +S+   
Sbjct: 4   KASDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQSETSLSMLHK 63

Query: 126 -----TRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKRQWKVQW 185
                 +W  PP  I  LN DA+W+D   +GGIGWI+R W+G  + AG R ++    V+ 
Sbjct: 64  TLNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKL 123

Query: 186 LETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLL--LSQDHDETDWVHFIQEAK 245
           LE  AI +GLR L  +      +   + +ETD+ +   LL    +D  +T WV  ++E  
Sbjct: 124 LEASAILEGLRNLTNLG-----VLRPLHIETDSAEVESLLNRKREDLTKTGWV--VEEIL 183

Query: 246 ALMERCNIEAEVHINRSHNQLAHQLAQKASLGGES 271
            L + C I A   + R  N  AH LAQ+AS+  ES
Sbjct: 184 NLRDSCEILAFAKVGRETNGCAHSLAQRASVLRES 211

[Close]

BLAST of Lcy12g008640 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 7.8e-19
Identity = 64/215 (29.77%), Postives = 103/215 (47.91%), Query Frame = 0


Query: 69  EKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIMSVT----- 128
           E+   +S+II WQIW+  N    K        ++  I++Y+      D  +   +     
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDL 65

Query: 129 ------------RWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKR 188
                       RW  P     KLN DA W  +   GGIGWILR   G  + A  R I+ 
Sbjct: 66  HLIRRIGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRT 125

Query: 189 QWKVQWLETLAISDGLRAL--EIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETDWVH 248
           +  + +LE +AI +GLRA+  E       +    + +E+D+L+ + LL  Q  D+T+ + 
Sbjct: 126 ERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIW 185

Query: 249 FIQEAKALMERCNIEAEVHINRSHNQLAHQLAQKA 265
            ++E   +ME   I +  HI+R  N++AH LA++A
Sbjct: 186 LLEEIWQMMEDMKIVSMRHISREANKVAHDLARRA 220

[Close]

BLAST of Lcy12g008640 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.0e-18
Identity = 62/213 (29.11%), Postives = 107/213 (50.23%), Query Frame = 0


Query: 69  EKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYL---------EEFKCGDEEI 128
           E+   +S+II WQIW+  N    K   P    ++  I++Y+          + K  ++++
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKSTNKDL 65

Query: 129 MSVTR--------WILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKR 188
             + R        W  P     KLN +A W  +   GGIGWILR   G  + A  R I+ 
Sbjct: 66  HLIRRIEDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRA 125

Query: 189 QWKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETDWVHFI 248
           +  + +LE +AI +GLRA+        +    + +E+D+L+ + LL  Q  D+T+ +  +
Sbjct: 126 ERNITYLEVMAICEGLRAIR------QEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLL 185

Query: 249 QEAKALMERCNIEAEVHINRSHNQLAHQLAQKA 265
           +E   +M+   I +  HI+R  N++AH LA++A
Sbjct: 186 EEIWQMMKDMEIVSMRHISREANKVAHGLARRA 212

[Close]

BLAST of Lcy12g008640 vs. ExPASy TrEMBL
Match: A0A7J6ERF5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020551 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 8.1e-16
Identity = 70/277 (25.27%), Postives = 114/277 (41.16%), Query Frame = 0


Query: 1   MDINPLCFMCRKKRETTEHLFWESKITKGIWDKFYPSSDLTCLDDRKGWGAKDYCEFLWR 60
           M I+P C +C  + ET  H  W     K IW K  P            W    +CE+L +
Sbjct: 103 MTIHPSCDLCGNQAETLTHALWSCSKIKPIW-KLVP------------W--YKHCEYLNQ 162

Query: 61  RCD-------GGRIEEKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQI-------E 120
                       ++       ++ I W IW++ N  ++     N   +   +        
Sbjct: 163 GSMFDILVSLKAKLNMNEFEDAIKIMWAIWENRNRKWNNLHVMNGAQLLDWVFSAYPGSS 222

Query: 121 KYLEEFKCGDEEIMSVTRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGF 180
             ++     + E+   T WI PP     +NCDA  N      G G+I R+W+G  L AG 
Sbjct: 223 TPVKNDMTTNMELSKTTHWIRPPAGYIAVNCDAAMNSGAAGIGTGFIWRNWDGKLLIAGM 282

Query: 181 RCIKRQWKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETD 240
               R   V   E  AI      LE ++   +     + +++D    V  +++QD+  T 
Sbjct: 283 NYSNRCCSVDMAEAWAI------LEALNNMPNTTDSPMEIQSDCKHLVDKIMNQDNHLTA 342

Query: 241 WVHFIQEAKALMERCNIEAEVHINRSHNQLAHQLAQK 264
               I + +  ME+ N  + VH+NR++N+ AH LA+K
Sbjct: 343 VNTIIHQIRMQMEKLNCNSIVHVNRNNNECAHMLARK 358

[Close]

BLAST of Lcy12g008640 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 110.5 bits (275), Expect = 2.2e-20
Identity = 66/222 (29.73%), Postives = 99/222 (44.59%), Query Frame = 0


Query: 11  RKKRETTEHLFWESKITKGIWDKFYPSSDLTCLDDRKGWGAKDYCEFLWRRCDGGRIEEK 70
           RKK ETT+H+ WE K+ K IW    P        DR  W  K+Y E+L  +       E+
Sbjct: 131 RKKEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWEWLMDKAG-----EE 190

Query: 71  SLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIM---------- 130
              +S+II  QIW+  N    K        ++  I++Y+      D  +           
Sbjct: 191 ERRRSMIIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSAGQDTNLKRKSKDFHPIR 250

Query: 131 -----SVTRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKRQWKV 190
                +  RW  P     KLN DA W  +    GIGWILR   G  +  G R I+ +  +
Sbjct: 251 RIGDNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNI 310

Query: 191 QWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLL 218
            +LE +AI +GLRA+        +    + +E+D+L+ + LL
Sbjct: 311 TYLEVMAICEGLRAIR------QEHCRPIHLESDSLEAIHLL 341

[Close]

BLAST of Lcy12g008640 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-20
Identity = 71/215 (33.02%), Postives = 108/215 (50.23%), Query Frame = 0


Query: 66  RIEEKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIMSV--- 125
           +  ++ L+  LI  W IW H N V  + +  +   M  Q+ K++ E     E  +S+   
Sbjct: 4   KASDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQSETSLSMLHK 63

Query: 126 -----TRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKRQWKVQW 185
                 +W  PP  I  LN DA+W+D   +GGIGWI+R W+G  + AG R ++    V+ 
Sbjct: 64  TLNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKL 123

Query: 186 LETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLL--LSQDHDETDWVHFIQEAK 245
           LE  AI +GLR L  +      +   + +ETD+ +   LL    +D  +T WV  ++E  
Sbjct: 124 LEASAILEGLRNLTNLG-----VLRPLHIETDSAEVESLLNRKREDLTKTGWV--VEEIL 183

Query: 246 ALMERCNIEAEVHINRSHNQLAHQLAQKASLGGES 271
            L + C I A   + R  N  AH LAQ+AS+  ES
Sbjct: 184 NLRDSCEILAFAKVGRETNGCAHSLAQRASVLRES 211

[Close]

BLAST of Lcy12g008640 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-18
Identity = 64/215 (29.77%), Postives = 103/215 (47.91%), Query Frame = 0


Query: 69  EKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIMSVT----- 128
           E+   +S+II WQIW+  N    K        ++  I++Y+      D  +   +     
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDL 65

Query: 129 ------------RWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKR 188
                       RW  P     KLN DA W  +   GGIGWILR   G  + A  R I+ 
Sbjct: 66  HLIRRIGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRT 125

Query: 189 QWKVQWLETLAISDGLRAL--EIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETDWVH 248
           +  + +LE +AI +GLRA+  E       +    + +E+D+L+ + LL  Q  D+T+ + 
Sbjct: 126 ERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIW 185

Query: 249 FIQEAKALMERCNIEAEVHINRSHNQLAHQLAQKA 265
            ++E   +ME   I +  HI+R  N++AH LA++A
Sbjct: 186 LLEEIWQMMEDMKIVSMRHISREANKVAHDLARRA 220

[Close]

BLAST of Lcy12g008640 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-18
Identity = 62/213 (29.11%), Postives = 107/213 (50.23%), Query Frame = 0


Query: 69  EKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYL---------EEFKCGDEEI 128
           E+   +S+II WQIW+  N    K   P    ++  I++Y+          + K  ++++
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKSTNKDL 65

Query: 129 MSVTR--------WILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKR 188
             + R        W  P     KLN +A W  +   GGIGWILR   G  + A  R I+ 
Sbjct: 66  HLIRRIEDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRA 125

Query: 189 QWKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETDWVHFI 248
           +  + +LE +AI +GLRA+        +    + +E+D+L+ + LL  Q  D+T+ +  +
Sbjct: 126 ERNITYLEVMAICEGLRAIR------QEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLL 185

Query: 249 QEAKALMERCNIEAEVHINRSHNQLAHQLAQKA 265
           +E   +M+   I +  HI+R  N++AH LA++A
Sbjct: 186 EEIWQMMKDMEIVSMRHISREANKVAHGLARRA 212

[Close]

BLAST of Lcy12g008640 vs. NCBI nr
Match: KAF4360260.1 (hypothetical protein F8388_020551 [Cannabis sativa] >KAF4393916.1 hypothetical protein G4B88_025885 [Cannabis sativa])

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-15
Identity = 70/277 (25.27%), Postives = 114/277 (41.16%), Query Frame = 0


Query: 1   MDINPLCFMCRKKRETTEHLFWESKITKGIWDKFYPSSDLTCLDDRKGWGAKDYCEFLWR 60
           M I+P C +C  + ET  H  W     K IW K  P            W    +CE+L +
Sbjct: 103 MTIHPSCDLCGNQAETLTHALWSCSKIKPIW-KLVP------------W--YKHCEYLNQ 162

Query: 61  RCD-------GGRIEEKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQI-------E 120
                       ++       ++ I W IW++ N  ++     N   +   +        
Sbjct: 163 GSMFDILVSLKAKLNMNEFEDAIKIMWAIWENRNRKWNNLHVMNGAQLLDWVFSAYPGSS 222

Query: 121 KYLEEFKCGDEEIMSVTRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGF 180
             ++     + E+   T WI PP     +NCDA  N      G G+I R+W+G  L AG 
Sbjct: 223 TPVKNDMTTNMELSKTTHWIRPPAGYIAVNCDAAMNSGAAGIGTGFIWRNWDGKLLIAGM 282

Query: 181 RCIKRQWKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETD 240
               R   V   E  AI      LE ++   +     + +++D    V  +++QD+  T 
Sbjct: 283 NYSNRCCSVDMAEAWAI------LEALNNMPNTTDSPMEIQSDCKHLVDKIMNQDNHLTA 342

Query: 241 WVHFIQEAKALMERCNIEAEVHINRSHNQLAHQLAQK 264
               I + +  ME+ N  + VH+NR++N+ AH LA+K
Sbjct: 343 VNTIIHQIRMQMEKLNCNSIVHVNRNNNECAHMLARK 358

[Close]

BLAST of Lcy12g008640 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 5.7e-14
Identity = 66/272 (24.26%), Postives = 117/272 (43.01%), Query Frame = 0


Query: 7   CFMCRKKRETTEHLFWESKITKGIWDKFYPSSDLTCLDDRKG--WGAKDYCEFLW--RRC 66
           C  C   +ET  HL ++    +  W        ++ +    G  W    Y    W     
Sbjct: 295 CIRCPSCKETVNHLLFKCTFARLTW-------AISSIPIPLGGEWADSIYVNLYWVFNLG 354

Query: 67  DGGRIEEKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFK-------CG 126
           +G    EK+      + W++WK+ N++  + ++ N + +  + E  LEE++       CG
Sbjct: 355 NGNPQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCG 414

Query: 127 DEEIM---SVTRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCIKRQ 186
            +  +   S  RW  PP +  K N DATWN + ++ GIGW+LR+  G     G R + + 
Sbjct: 415 TKPQVNRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKL 474

Query: 187 WKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQDHDETDWVHFIQ 246
             V   E  A+   + +L     +       V  E+D+ Q +  +L+ D         IQ
Sbjct: 475 KSVLEAELEAMRWAVLSLSRFQYN------YVIFESDS-QVLIEILNNDEIWPSLKPTIQ 534

Query: 247 EAKALMERCNIEAEVHINRSHNQLAHQLAQKA 265
           + + L+ +      V I R  N LA ++A+++
Sbjct: 535 DLQRLLSQFTEVKFVFIPREGNTLAERVARES 552

[Close]

BLAST of Lcy12g008640 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-12
Identity = 61/232 (26.29%), Postives = 95/232 (40.95%), Query Frame = 0


Query: 7   CFMCRKKRETTEHLFWESKITKGIWD----KFYPSSDLTCLDDRKGWGAKDYCEFLWRRC 66
           C  C   RET  HL ++    + +W       YP  +         W    Y    W   
Sbjct: 12  CVRCPDSRETVNHLLFKCCFARLVWAISPIPAYPEGE---------WTDSLYANLYWVLN 71

Query: 67  DGGRIEEKSLNKSLI--ICWQIWKHTNDVYHK-KQQPNQEAMKHQIEKYLE-------EF 126
               I +     +L+  + W++WK  N++  K K+    E ++  +E + E       E 
Sbjct: 72  LEVEIPKLGKIGNLVPWLLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEG 131

Query: 127 KCGDEEI---MSVTRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAGFRCI 186
           K    ++   +SV +W  PP +  K N DATW  E  + GIGWILR+ +G  L  G R +
Sbjct: 132 KASGPQVERNLSV-QWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARAL 191

Query: 187 KRQWKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQD 222
            R   V   E  A+   +  +   +        ++  E+DA   V LL S D
Sbjct: 192 PRTKNVLEAELEALRWAVLTMSRFN------YKRIIFESDAQALVNLLNSDD 227

[Close]

BLAST of Lcy12g008640 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 38/179 (21.23%), Postives = 77/179 (43.02%), Query Frame = 0


Query: 4   NPLCFMCRKKRETTEHLFWESKITKGIW-DKFYPSSDLTCLDDRKGWGAKDYCEFLWRRC 63
           +P C  C ++ ET++HLF++    + +W     P  +L       G   +   E L   C
Sbjct: 53  HPQCHRCCQEDETSQHLFFDCFYAQQVWRASGIPHQELR----TTGITMETKMELLLSSC 112

Query: 64  DGGRIEEKSLNKSLIICWQIWKHTNDVYHKKQQPNQEAMKHQIEKYLEEFKCGDEEIMSV 123
              R + +  N ++ I W++WK  N +  +++  + +    +    ++E++  +  + S+
Sbjct: 113 LANR-QPQLFNLAIWILWRLWKSRNQLVFQQKSISWQNTLQRARNDVQEWEDTNTYVQSL 172

Query: 124 ----------------TRWILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAG 166
                           T+W  PP    K N D  +N + +    GW++R  NG  + +G
Sbjct: 173 NQQVHSSRHQQPTMARTKWQRPPSTWIKYNYDGAFNHQTRNAKAGWLMRDENGVYMGSG 226

[Close]

BLAST of Lcy12g008640 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 2.2e-05
Identity = 63/277 (22.74%), Postives = 112/277 (40.43%), Query Frame = 0


Query: 1   MDINPLCFMCRKKRETTEHLFWESKITKGIWDKFYPSSDLTCLDDRKGWGAKDYCEFLWR 60
           M I+P C  C ++ E+  H  +        W      SD + +  R    + D+ E +  
Sbjct: 194 MRIDPSCPRCHRENESINHALFTCPFATMAW----RLSDSSLI--RNQLMSNDFEENISN 253

Query: 61  RCDGGRIEEKSLNKSLI---ICWQIWKHTNDVYHKK--QQPNQEAM--KHQIEKYLEEFK 120
             +  +    S    L+   + W+IWK  N+V   K  + P++  +  K +   +L   +
Sbjct: 254 ILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQ 313

Query: 121 CGDEEIMSVTR--------WILPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAG 180
              ++  S TR        W  PP    K N DA ++ ++ +   GWI+R+  G+P+S G
Sbjct: 314 -SHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWG 373

Query: 181 FRCIKRQWKVQWLETLAISDGLRALEIMSGDIHDLALQVRVETDALQTVKLLLSQDHDET 240
              +         ET A+   L+   I          QV +E D  QT+  L++     +
Sbjct: 374 SMKLAHTSNPLEAETKALLAALQQTWIRG------YTQVFMEGDC-QTLINLINGISFHS 433

Query: 241 DWVHFIQEAKALMERCNIEAEVHINRSHNQLAHQLAQ 263
              + +++      +        I R  N+LAH LA+
Sbjct: 434 SLANHLEDISFWANKFASIQFGFIRRKGNKLAHVLAK 456

[Close]

BLAST of Lcy12g008640 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.4e-04
Identity = 25/99 (25.25%), Postives = 45/99 (45.45%), Query Frame = 0


Query: 78  ICWQIWKHTNDVY----HKKQQPNQEAMKHQIEKYLEEFKCGDE-------EIMSVTRWI 137
           + W+IWK  ND+       K Q   E   +  +++L+     ++       +    T+W 
Sbjct: 47  LMWRIWKSGNDLVFNHTRTKFQTTVEMALNDTKEWLDNTMTNEQQNGNRNADPSRNTKWS 106

Query: 138 LPPEEICKLNCDATWNDERKQGGIGWILRHWNGSPLSAG 166
            P  +  K N DA+ ++     G+GWILR+  G+ +  G
Sbjct: 107 PPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECG 145

The following BLAST results are available for this feature:

BLAST of Lcy12g008640 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy12g008640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DL64	1.1e-20	29.73	uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DNV9	1.4e-20	33.02	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DSV1	7.8e-19	29.77	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CP26	1.0e-18	29.11	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A7J6ERF5	8.1e-16	25.27	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020551 PE=4 SV=1	[more]

BLAST of Lcy12g008640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154990.1	2.2e-20	29.73	uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]	[more]
XP_022155262.1	2.9e-20	33.02	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022156777.1	1.6e-18	29.77	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143535.1	2.1e-18	29.11	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
KAF4360260.1	1.7e-15	25.27	hypothetical protein F8388_020551 [Cannabis sativa] >KAF4393916.1 hypothetical p...	[more]

BLAST of Lcy12g008640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	5.7e-14	24.26	Ribonuclease H-like superfamily protein	[more]
AT2G34320.1	3.1e-12	26.29	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G25270.1	1.9e-09	21.23	Ribonuclease H-like superfamily protein	[more]
AT3G09510.1	2.2e-05	22.74	Ribonuclease H-like superfamily protein	[more]
AT5G65005.1	2.4e-04	25.25	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 132..265 e-value: 5.4E-10 score: 41.4
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 136..264 e-value: 1.2E-14 score: 54.1
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 77..258
None	No IPR available	PANTHER	PTHR47074:SF3	OS07G0513450 PROTEIN	coord: 77..258
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 135..261 e-value: 8.2824E-14 score: 64.6428
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 133..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g008640.1	Lcy12g008640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity