Lcy12g006250 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g006250 vs. ExPASy Swiss-Prot
Match: B7SY83 (FHA domain-containing protein PS1 OS=Arabidopsis thaliana OX=3702 GN=PS1 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.2e-99
Identity = 435/1482 (29.35%), Postives = 646/1482 (43.59%), Query Frame = 0

Query: 14   EMKIPLFTVLKNGAILKNIFIVN--------------NVADRENEEVIILGRHPDCNIML 73
            E  IP+FTVLKNGAILKNIF+VN              +  D E EE++++GRHPDC+I+L
Sbjct: 17   EKTIPVFTVLKNGAILKNIFVVNSRDFSSPERNGSTVSDDDGEVEEILVVGRHPDCDILL 76

Query: 74   THPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSR 133
            THPSISRFHL+I S  S QKL V DLSSVHGTWV   RIE    VE+ EGDT+R+GGS+R
Sbjct: 77   THPSISRFHLEIRSISSRQKLFVTDLSSVHGTWVRDLRIEPHGCVEVEEGDTIRIGGSTR 136

Query: 134  VYKLHWVPLSCAYDFQCP---------KEKKEEKVAIIEEEAVEDCEK------------ 193
            +Y+LHW+PLS AYD   P           ++EE+  ++E E +E  +             
Sbjct: 137  IYRLHWIPLSRAYDLDNPFVSPLDASTVLEQEEENRMLEAENLEVAQHQSLENTTSGDEG 196

Query: 194  ----DI------------------------------SLLDENKETVEDSVLENIEPLYSD 253
                D+                              S+L   +++VE   L+  E L + 
Sbjct: 197  VLHLDVTSEGTGSSVPSEDEDTYVTTREMSMPVASPSVLTLVRDSVETQKLQFNEDLQTS 256

Query: 254  ENWNMEMKEVPLAAPL------------GEVEGMVMSSV-------------DGGESVND 313
              W++++ E  +A  L            G+VEG+  S +             DGG  +N 
Sbjct: 257  PKWDLDVIE-SVAEKLSGSFVRSTQQSGGDVEGLGCSELFDAAEADECDVRGDGGLHLNV 316

Query: 314  LRNECKK--------------EEISLLSKPFGDE------------------------LK 373
            +  + +               +  SL +   GD+                        + 
Sbjct: 317  ISEKMESSVPNMIEAENLEVAQHQSLANTALGDDEDLHLDVTSEGTGSSVPSEDEDTYIT 376

Query: 374  SLEMSPQPPFLPLSAENL------SFNVESIIMSSFF-------------GSESKSSS-- 433
            ++E+S     +PL++ N+      S   + +  +  F              +E  SSS  
Sbjct: 377  TMEIS-----VPLASPNVLTLARDSIKTQKLQSTQDFQTPTMWDLDVVEAAAEKPSSSCV 436

Query: 434  --------------------SSTFEQNDSSSIWNIPLENVS---RSSLSEKQQCQSKIES 493
                                ++  ++ D     ++ L  +S    SS+S K+     + +
Sbjct: 437  LGKKLSGGYVEELGCFELFVAAEADKCDVRGDGSLHLNEISERMESSMSNKED-DPFLAA 496

Query: 494  PQPPSLPLSAENLS----FNVENIIMSSFF-------DAETKSSSANVFERE-----ETS 553
             +  SLPLS + ++    + VE++  S  F       +AE  SS  +    +     ETS
Sbjct: 497  KETSSLPLSTDFINPETLWLVEDVQASPEFCTSSVKANAENPSSGCSPSTEQIDGCFETS 556

Query: 554  SIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAMIA 613
                  L  +   LS   +++  +E   +++   +  P  ++ +   E + + ED+  + 
Sbjct: 557  GCSAFDLAAEVESLSLHQEVSEETEFVTKEVMGVSSEPLGKADIRSHEENGESEDSRQVI 616

Query: 614  FA---------------GGECDSS------SINEFEQKE-----INNVMTIPLGHELSNV 673
                              GE + S      S   F + E     +       +G E  + 
Sbjct: 617  EVSAEPVAKADIQSHEENGETEGSRQVIEVSPKSFSEAEPTIEILTGEAQGIIGSEFPSE 676

Query: 674  ALVDEEKLILDHERQQFDNENGQLLAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLAL 733
              V+ E   L H++   + +N ++ + +++    +  E   + P +    SS + P   L
Sbjct: 677  LAVETESENLLHQKSIGETKN-EIRSHEDYGETEDYGETECSWPDIAVSPSSVSPPEPTL 736

Query: 734  QSKTVTEK--------KEFMVEHELHPLEDDHSSVTTEKGI------QEMEILNK----- 793
            +  T   +         E  VE E+  L    S+V T+  I       E E+  +     
Sbjct: 737  EILTDEARGLLGSEFLSEVTVETEIENLLHQKSNVETKADILIHEDYGETEVSRQIITVS 796

Query: 794  ----SQAE---------------VGEDCVDKGNEELYQVTEC------------------ 853
                S+AE               VG D   +   E+   TEC                  
Sbjct: 797  PNSFSKAEPTLETEDSRQQARGLVGSD--SEFQSEVAMKTECENLLNQKRNGETKVSSRQ 856

Query: 854  ---LLDCQSQYK---------------NDSSMKTTSEVTPNSTMNQNVEYCIE-EKYSTS 913
               + DC S  K               + S   + SEV P +  +Q      E EK  T 
Sbjct: 857  ASPVSDCLSTPKDRLSSINTDDIQSLCSSSQPPSESEVNPATDQDQESGIISETEKPKTE 916

Query: 914  L----ETIEPSKSVPEKDYVHKEQLSEPSFVPCATEFVYSSLPD----EEVSPKIDVQIE 973
            L       E   S+ E +      +   S  P  +     +  D    EE+S       E
Sbjct: 917  LLIGSGRSEKYYSLSEIEGEENTDIGRLSRCPIPSALAAKTSEDTKLIEELSSSDSGSQE 976

Query: 974  NQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVD--RANRVGTTLMDEIN 1033
            NQTP+ +  V   +  +  S+   +IWSRRGK  S  +I T+    +  + G    D+++
Sbjct: 977  NQTPETHA-VRDDVLCDMDSSSTCNIWSRRGKAASVLKIRTNKSQGKQKQTGRQPKDKLH 1036

Query: 1034 HEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKANIEDS-GNFSRSS 1093
             +    ++  +  + H     E EIFTPDKEN TP++ ++K L+   +++DS  +   S 
Sbjct: 1037 RKQALSDKSISLTIHHGAEILEPEIFTPDKENLTPSSHMLKRLQDIGDVKDSKSSLKLSG 1096

Query: 1094 KSQTSIFKSRHKVKQEEELSEES----DKENQTP-------------------------- 1137
            KS +S+  S   V   E  +E      DKEN TP                          
Sbjct: 1097 KSCSSLVHSSIAVLASEAFTEPEIFTPDKENLTPSSHMLKRLREFGDIKDTKGSSSKATR 1156

BLAST of Lcy12g006250 vs. ExPASy Swiss-Prot
Match: Q9FIK2 (Protein phosphatase 1 regulatory inhibitor subunit PPP1R8 homolog OS=Arabidopsis thaliana OX=3702 GN=At5g47790 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.8e-08
Identity = 40/105 (38.10%), Postives = 57/105 (54.29%), Query Frame = 0

Query: 22  VLKNGAILKNIFIVNNVADRENEEVIILGR-HPDCNIMLTHPSISRFHLQI--HSNPSSQ 81
           V+K+G IL  I +     DR      I GR H  C+ +L H S+SR H  +  H N S  
Sbjct: 70  VVKDGQILDRIHL-----DRRRH---IFGRQHQTCDFVLDHQSVSRQHAAVVPHKNGS-- 129

Query: 82  KLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKL 124
            + V+DL S HGT+V+ +R+     VE+  G +LR   S+R+Y L
Sbjct: 130 -IFVIDLGSAHGTFVANERLTKDTPVELEVGQSLRFAASTRIYLL 163

BLAST of Lcy12g006250 vs. ExPASy Swiss-Prot
Match: P34648 (Uncharacterized protein ZK632.2 OS=Caenorhabditis elegans OX=6239 GN=ZK632.2 PE=4 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 5.2e-05
Identity = 41/160 (25.62%), Postives = 77/160 (48.12%), Query Frame = 0

Query: 20  FTVLKNGAILKNIFIVNNVADRENEEVIILGR-HPDCNIMLTHPSISRFH--LQIHSNPS 79
           F +LK G ++ +     ++++R+N   +++GR  P C++++ HPSISR+H  LQ  ++  
Sbjct: 85  FEILKEGKLIASY----DLSNRKNSTFVVIGRIKPGCDLLMEHPSISRYHCILQYGNDKM 144

Query: 80  SQK---LCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKLHWVPLSCAYD 139
           S+      + +L S HG+ ++ KR+     +  R G   + G S+R+            +
Sbjct: 145 SKTGKGWHIFELGSTHGSRMNKKRLPPKQYIRTRVGFIFQFGESTRILNFVGPEEDSEPE 204

Query: 140 FQC-PKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVED 173
           + C P E K  K     E  +        + DE +E  E+
Sbjct: 205 WDCSPTEMKLRKHKKELEAKLRAAAAQEMIDDEKREKEEE 240

BLAST of Lcy12g006250 vs. ExPASy Swiss-Prot
Match: Q28147 (Nuclear inhibitor of protein phosphatase 1 OS=Bos taurus OX=9913 GN=PPP1R8 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 6.8e-05
Identity = 28/107 (26.17%), Postives = 55/107 (51.40%), Query Frame = 0

Query: 22  VLKNGAILKNIFIVNNVADRENEEVIILGRHPD-CNIMLTHPSISRFHLQIHSNPSSQKL 81
           V+K   +++ + I       + ++  + GR+PD C+  + H S SR H  +  +   +++
Sbjct: 31  VVKGDKLIEKLII-------DEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRV 90

Query: 82  CVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKLHWVP 128
            ++DL+S HGT++   R+E     ++    T+  G S+R Y L   P
Sbjct: 91  FLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKP 130

BLAST of Lcy12g006250 vs. ExPASy Swiss-Prot
Match: Q12972 (Nuclear inhibitor of protein phosphatase 1 OS=Homo sapiens OX=9606 GN=PPP1R8 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 6.8e-05
Identity = 28/107 (26.17%), Postives = 55/107 (51.40%), Query Frame = 0

Query: 22  VLKNGAILKNIFIVNNVADRENEEVIILGRHPD-CNIMLTHPSISRFHLQIHSNPSSQKL 81
           V+K   +++ + I       + ++  + GR+PD C+  + H S SR H  +  +   +++
Sbjct: 31  VVKGDKLIEKLII-------DEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRV 90

Query: 82  CVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKLHWVP 128
            ++DL+S HGT++   R+E     ++    T+  G S+R Y L   P
Sbjct: 91  FLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKP 130

BLAST of Lcy12g006250 vs. ExPASy TrEMBL
Match: A0A6J1C011 (FHA domain-containing protein PS1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007231 PE=4 SV=1)

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 817/1154 (70.80%), Postives = 909/1154 (78.77%), Query Frame = 0

Query: 1    MADR-EGQSNPDQNEMKIPLFTVLKNGAILKNIFIVNNVADREN-EEVIILGRHPDCNIM 60
            MADR E +S PD N+ KIP+FTVLKNGAILKNIFI+NN  +  N EEVIILGRHPDCNIM
Sbjct: 1    MADREEEESKPDLNDKKIPVFTVLKNGAILKNIFILNNFPNPGNEEEVIILGRHPDCNIM 60

Query: 61   LTHPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSS 120
            LTHPSISRFHLQIHSNPSS KLC+VDLSSVHGTWVSGK+IEAG RVEMREG+TLRVGGSS
Sbjct: 61   LTHPSISRFHLQIHSNPSSLKLCLVDLSSVHGTWVSGKKIEAGARVEMREGNTLRVGGSS 120

Query: 121  RVYKLHWVPLSCAYDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENI 180
            R+Y+LHWVPLS  YDF+   EKKEE+VAI EE AVE+CEK+ SLLDE KETV+D    NI
Sbjct: 121  RIYRLHWVPLSRVYDFEI--EKKEEEVAIAEERAVENCEKENSLLDEKKETVKDLFFGNI 180

Query: 181  -EPLYSDENWNME-MKEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPF 240
             EPLYSDE+W++E MKEV  A P+GE EGMV+S VDGGES + LRNEC +EE  LLSKPF
Sbjct: 181  VEPLYSDEDWSIEMMKEVTSAPPMGEFEGMVVSPVDGGESESHLRNECPQEETCLLSKPF 240

Query: 241  GDELKSLEMSPQPPFLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIP 300
            G+E KSL       F PLSAENLSFNVE+IIMSSFFGSE+KS          +SSIW +P
Sbjct: 241  GNESKSL-------FAPLSAENLSFNVENIIMSSFFGSENKS----------TSSIWTMP 300

Query: 301  LENVSRSSLSEKQQ-CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVF 360
            LEN SR S S  QQ      ESPQ  SLPLSAENLSFNVENIIMSSFF +E+KSSS+N F
Sbjct: 301  LENESRISSSGNQQLLHHGKESPQSVSLPLSAENLSFNVENIIMSSFFSSESKSSSSNTF 360

Query: 361  EREETSSIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVE 420
            ++EET   WTI LENKS  LS VDD      NGY+Q DNEN+SPQ  S  LCKE+SS+VE
Sbjct: 361  DQEETDRSWTIPLENKSKNLSVVDDRKPAPANGYRQFDNENQSPQTHSVPLCKENSSNVE 420

Query: 421  DTAMIAFAGGECDSSSINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNE 480
            D  M               FEQKEI+++MTIPLGHEL++ A+ D+ K I  HE Q FD E
Sbjct: 421  DNDM---------------FEQKEISSIMTIPLGHELTDEAVADDAKPI-SHESQNFDLE 480

Query: 481  N----GQLLAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHE 540
            N      LL+ KN SS+ E     QNL  + EV S        ++  T+TEK+E MVEHE
Sbjct: 481  NESPQSVLLSGKNLSSDVET----QNL-SICEVNSLIYDVGNVVE--TITEKEELMVEHE 540

Query: 541  LHPLEDDHSSVTTEKGIQEMEILNKSQAEVGEDCVDKGNEEL---YQVTECLLDCQSQYK 600
            LHPLE +HSSV+TE+ I E E L+K + EV E  VD+GN +    YQV E LL+ QSQY 
Sbjct: 541  LHPLEYNHSSVSTEERIHETETLDKIETEVEEVNVDEGNGDFYHTYQVKESLLNWQSQYI 600

Query: 601  NDSSMKTTSEVTPNSTMNQNVEYCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEPSFVP 660
            N++SMK+T EV PN  MNQNV  C+EEK +   ET++P+KSV EKD+  KE LSEP  V 
Sbjct: 601  NETSMKSTPEVIPNLPMNQNVGCCVEEKDTAGYETLKPTKSVQEKDHEQKE-LSEPGTVS 660

Query: 661  CATEFVYSSLPDEEVSPKIDVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSF 720
             + EFV SSLPDE+VS +I V+ E QTP          RS+N+SA+G SIW RRGKP SF
Sbjct: 661  SSMEFVNSSLPDEDVSLEISVEKEFQTP----------RSDNVSAMGDSIWLRRGKPNSF 720

Query: 721  PRIETDVDRANRVGTTLMDEINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTL 780
            PRIET V+RANRVGT+L DEINHEI  DE V NTLL  L GEEEEEIFTPDKENFTPNTL
Sbjct: 721  PRIETGVNRANRVGTSLTDEINHEIAEDETVVNTLLPIL-GEEEEEIFTPDKENFTPNTL 780

Query: 781  LMKSLKKKANIEDS-GNFSRSSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKL 840
            LMKSLKKKANIEDS  N SR SKSQ+SIFKSR K+K  EELSEESDKENQTPR LQEQKL
Sbjct: 781  LMKSLKKKANIEDSVKNSSRPSKSQSSIFKSRQKIK-PEELSEESDKENQTPRFLQEQKL 840

Query: 841  AKQMSKNRTFGKEKIVIKKGGAERAPFQSLQSNLAEKKRLEASIVKSARKSNISVSTGAM 900
            AK +SKNR FGK+K V+K+GG ERAPFQSLQSNLA KKR E ++V SARKSNISV TGAM
Sbjct: 841  AKSISKNRRFGKDKKVMKRGGGERAPFQSLQSNLAGKKRPEVTVVNSARKSNISVCTGAM 900

Query: 901  KNKFTEEGKKRWTMVVDINSLLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSL 960
            KNKFT EGKKRWTMVVDINSLLNKESMKSL+LLQGLQGTHLI+PRMVIRELDCLRRH SL
Sbjct: 901  KNKFT-EGKKRWTMVVDINSLLNKESMKSLKLLQGLQGTHLIVPRMVIRELDCLRRHGSL 960

Query: 961  FRKTTDAASILQWIEDCIVKTRWWIHVQNSGE-GEPPATPPATPQSPCTEGSSQSLFWRT 1020
            FRKTT+AASILQWIEDC+V+TRWWIHVQ+S E G PPAT PATP+SP TEG SQSL WRT
Sbjct: 961  FRKTTEAASILQWIEDCMVQTRWWIHVQSSDEGGRPPATSPATPRSPYTEG-SQSLLWRT 1020

Query: 1021 ASSIQSITQRSFMEALSPTPEDHILDSALHFRRGVKHGQLVLLSDDVTLKIKAMAEGLIC 1080
            ASSIQSITQRSFME LSPT EDHIL+ ALHFRRGV HGQLVLLSDDVTLKIKAMAEGLIC
Sbjct: 1021 ASSIQSITQRSFMEVLSPTAEDHILECALHFRRGVNHGQLVLLSDDVTLKIKAMAEGLIC 1080

Query: 1081 ETAKEFRESLVNPFSERFLWADSSPRGLTWSCSDDVVLRERYDRCPSRSSKG-AEGAKGL 1140
            ETAKEFRESLVNPFSERFLWADSSPRGLTWSC DDVVLRERYDRC S S KG AEGAKGL
Sbjct: 1081 ETAKEFRESLVNPFSERFLWADSSPRGLTWSCPDDVVLRERYDRCSSWSLKGTAEGAKGL 1097

BLAST of Lcy12g006250 vs. ExPASy TrEMBL
Match: A0A6J1C0J1 (FHA domain-containing protein PS1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007231 PE=4 SV=1)

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 817/1158 (70.55%), Postives = 909/1158 (78.50%), Query Frame = 0

Query: 1    MADR-EGQSNPDQNEMKIPLFTVLKNGAILKNIFIVNNVADREN-EEVIILGRHPDCNIM 60
            MADR E +S PD N+ KIP+FTVLKNGAILKNIFI+NN  +  N EEVIILGRHPDCNIM
Sbjct: 1    MADREEEESKPDLNDKKIPVFTVLKNGAILKNIFILNNFPNPGNEEEVIILGRHPDCNIM 60

Query: 61   LTHPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSS 120
            LTHPSISRFHLQIHSNPSS KLC+VDLSSVHGTWVSGK+IEAG RVEMREG+TLRVGGSS
Sbjct: 61   LTHPSISRFHLQIHSNPSSLKLCLVDLSSVHGTWVSGKKIEAGARVEMREGNTLRVGGSS 120

Query: 121  RVYKLHWVPLSCAYDFQCPKEKKEEKVAIIEEEAVEDCE----KDISLLDENKETVEDSV 180
            R+Y+LHWVPLS  YDF+   EKKEE+VAI EE AVE+CE    K+ SLLDE KETV+D  
Sbjct: 121  RIYRLHWVPLSRVYDFEI--EKKEEEVAIAEERAVENCEIVLQKENSLLDEKKETVKDLF 180

Query: 181  LENI-EPLYSDENWNME-MKEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLL 240
              NI EPLYSDE+W++E MKEV  A P+GE EGMV+S VDGGES + LRNEC +EE  LL
Sbjct: 181  FGNIVEPLYSDEDWSIEMMKEVTSAPPMGEFEGMVVSPVDGGESESHLRNECPQEETCLL 240

Query: 241  SKPFGDELKSLEMSPQPPFLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSI 300
            SKPFG+E KSL       F PLSAENLSFNVE+IIMSSFFGSE+KS          +SSI
Sbjct: 241  SKPFGNESKSL-------FAPLSAENLSFNVENIIMSSFFGSENKS----------TSSI 300

Query: 301  WNIPLENVSRSSLSEKQQ-CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSS 360
            W +PLEN SR S S  QQ      ESPQ  SLPLSAENLSFNVENIIMSSFF +E+KSSS
Sbjct: 301  WTMPLENESRISSSGNQQLLHHGKESPQSVSLPLSAENLSFNVENIIMSSFFSSESKSSS 360

Query: 361  ANVFEREETSSIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESS 420
            +N F++EET   WTI LENKS  LS VDD      NGY+Q DNEN+SPQ  S  LCKE+S
Sbjct: 361  SNTFDQEETDRSWTIPLENKSKNLSVVDDRKPAPANGYRQFDNENQSPQTHSVPLCKENS 420

Query: 421  SDVEDTAMIAFAGGECDSSSINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQ 480
            S+VED  M               FEQKEI+++MTIPLGHEL++ A+ D+ K I  HE Q 
Sbjct: 421  SNVEDNDM---------------FEQKEISSIMTIPLGHELTDEAVADDAKPI-SHESQN 480

Query: 481  FDNEN----GQLLAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFM 540
            FD EN      LL+ KN SS+ E     QNL  + EV S        ++  T+TEK+E M
Sbjct: 481  FDLENESPQSVLLSGKNLSSDVET----QNL-SICEVNSLIYDVGNVVE--TITEKEELM 540

Query: 541  VEHELHPLEDDHSSVTTEKGIQEMEILNKSQAEVGEDCVDKGNEEL---YQVTECLLDCQ 600
            VEHELHPLE +HSSV+TE+ I E E L+K + EV E  VD+GN +    YQV E LL+ Q
Sbjct: 541  VEHELHPLEYNHSSVSTEERIHETETLDKIETEVEEVNVDEGNGDFYHTYQVKESLLNWQ 600

Query: 601  SQYKNDSSMKTTSEVTPNSTMNQNVEYCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEP 660
            SQY N++SMK+T EV PN  MNQNV  C+EEK +   ET++P+KSV EKD+  KE LSEP
Sbjct: 601  SQYINETSMKSTPEVIPNLPMNQNVGCCVEEKDTAGYETLKPTKSVQEKDHEQKE-LSEP 660

Query: 661  SFVPCATEFVYSSLPDEEVSPKIDVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGK 720
              V  + EFV SSLPDE+VS +I V+ E QTP          RS+N+SA+G SIW RRGK
Sbjct: 661  GTVSSSMEFVNSSLPDEDVSLEISVEKEFQTP----------RSDNVSAMGDSIWLRRGK 720

Query: 721  PTSFPRIETDVDRANRVGTTLMDEINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFT 780
            P SFPRIET V+RANRVGT+L DEINHEI  DE V NTLL  L GEEEEEIFTPDKENFT
Sbjct: 721  PNSFPRIETGVNRANRVGTSLTDEINHEIAEDETVVNTLLPIL-GEEEEEIFTPDKENFT 780

Query: 781  PNTLLMKSLKKKANIEDS-GNFSRSSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQ 840
            PNTLLMKSLKKKANIEDS  N SR SKSQ+SIFKSR K+K  EELSEESDKENQTPR LQ
Sbjct: 781  PNTLLMKSLKKKANIEDSVKNSSRPSKSQSSIFKSRQKIK-PEELSEESDKENQTPRFLQ 840

Query: 841  EQKLAKQMSKNRTFGKEKIVIKKGGAERAPFQSLQSNLAEKKRLEASIVKSARKSNISVS 900
            EQKLAK +SKNR FGK+K V+K+GG ERAPFQSLQSNLA KKR E ++V SARKSNISV 
Sbjct: 841  EQKLAKSISKNRRFGKDKKVMKRGGGERAPFQSLQSNLAGKKRPEVTVVNSARKSNISVC 900

Query: 901  TGAMKNKFTEEGKKRWTMVVDINSLLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRR 960
            TGAMKNKFT EGKKRWTMVVDINSLLNKESMKSL+LLQGLQGTHLI+PRMVIRELDCLRR
Sbjct: 901  TGAMKNKFT-EGKKRWTMVVDINSLLNKESMKSLKLLQGLQGTHLIVPRMVIRELDCLRR 960

Query: 961  HSSLFRKTTDAASILQWIEDCIVKTRWWIHVQNSGE-GEPPATPPATPQSPCTEGSSQSL 1020
            H SLFRKTT+AASILQWIEDC+V+TRWWIHVQ+S E G PPAT PATP+SP TEG SQSL
Sbjct: 961  HGSLFRKTTEAASILQWIEDCMVQTRWWIHVQSSDEGGRPPATSPATPRSPYTEG-SQSL 1020

Query: 1021 FWRTASSIQSITQRSFMEALSPTPEDHILDSALHFRRGVKHGQLVLLSDDVTLKIKAMAE 1080
             WRTASSIQSITQRSFME LSPT EDHIL+ ALHFRRGV HGQLVLLSDDVTLKIKAMAE
Sbjct: 1021 LWRTASSIQSITQRSFMEVLSPTAEDHILECALHFRRGVNHGQLVLLSDDVTLKIKAMAE 1080

Query: 1081 GLICETAKEFRESLVNPFSERFLWADSSPRGLTWSCSDDVVLRERYDRCPSRSSKG-AEG 1140
            GLICETAKEFRESLVNPFSERFLWADSSPRGLTWSC DDVVLRERYDRC S S KG AEG
Sbjct: 1081 GLICETAKEFRESLVNPFSERFLWADSSPRGLTWSCPDDVVLRERYDRCSSWSLKGTAEG 1101

BLAST of Lcy12g006250 vs. ExPASy TrEMBL
Match: A0A6J1I652 (FHA domain-containing protein PS1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471434 PE=4 SV=1)

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 761/1131 (67.29%), Postives = 842/1131 (74.45%), Query Frame = 0

Query: 12   QNEMKIPLFTVLKNGAILKNIFIVNNVADRENEEVIILGRHPDCNIMLTHPSISRFHLQI 71
            + E KIP+FTVLKNGAILKN+F+VNNV DRENEE I+LGRHPDCNIMLTHPSISRFHLQI
Sbjct: 4    KEEEKIPVFTVLKNGAILKNLFLVNNVTDRENEETIVLGRHPDCNIMLTHPSISRFHLQI 63

Query: 72   HSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKLHWVPLSCA 131
            H NPS  K+ VVDLSSVHGTWVSGKRIEAG RVEMREGDTL VGGSSRVYKLHWVPL  A
Sbjct: 64   HLNPSLLKVFVVDLSSVHGTWVSGKRIEAGARVEMREGDTLSVGGSSRVYKLHWVPLRDA 123

Query: 132  YDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENIEPLYSDENWNMEM 191
            YDF+CPKEKKEE++AIIEE+AVEDCEK+ISL DENKETVEDSV ++I P YSDENWNME+
Sbjct: 124  YDFECPKEKKEEELAIIEEKAVEDCEKEISLSDENKETVEDSVSDSIVPFYSDENWNMEL 183

Query: 192  -KEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPFGDELKSLEMSPQPP 251
             KEV +A   GEVEGMV+SS           NEC KEE SL S PFG+ELKSLEM     
Sbjct: 184  IKEVSVAPLSGEVEGMVVSSF----------NECGKEETSLFSIPFGNELKSLEM----- 243

Query: 252  FLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIPLENVSRSSLSEKQQ 311
                                                                        
Sbjct: 244  ------------------------------------------------------------ 303

Query: 312  CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVFEREETSSIWTIRLEN 371
                  S Q P LPLSA+NLSFNVENIIMSS FD+ET+SSS N+FE EET+         
Sbjct: 304  ------SLQSPCLPLSADNLSFNVENIIMSSIFDSETESSSCNMFEWEETN--------- 363

Query: 372  KSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAMIAFAGGECDSS 431
             SNI   VD IT TSENGYQQLDNE +S QP S   C+ + SD+EDTAMIAFAGG+ +SS
Sbjct: 364  -SNIFPVVDVIT-TSENGYQQLDNEKESLQPPSMSPCEGNFSDIEDTAMIAFAGGKYESS 423

Query: 432  SINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNENGQLLAEKNFSSNAE 491
            S NEFEQ EI            SNVA+VD+EK       Q FD E+ Q+LAE+N SSN  
Sbjct: 424  SRNEFEQIEI------------SNVAVVDDEK------PQPFDTESRQVLAERNSSSNVG 483

Query: 492  VVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHELHPLEDDHSSVTTEKGIQE 551
               EIQ LPP  +VK           S+T TEK EFM EHELHPL+D+ SS++TEK +QE
Sbjct: 484  DGVEIQYLPPDQKVK-----------SETSTEKIEFMEEHELHPLDDNRSSISTEKCLQE 543

Query: 552  MEILNKSQAEVGEDCVDKGNEELYQVTECLLDCQSQYKNDSSMKTTSEVTPNSTMNQNVE 611
            +E+L+K Q ++ ED  D+ NEELY+VTECLLDCQ+QYKNDSSMKTTSEVTPN    QNVE
Sbjct: 544  VEMLSKIQTKIEEDYADERNEELYKVTECLLDCQAQYKNDSSMKTTSEVTPNLPTKQNVE 603

Query: 612  YCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEPSFVPCATEFVYSSLPDEEVSPKIDVQ 671
             C+EEKY+  LET EPSKSV EKDY HKE LSEPSFVP AT++            KI V+
Sbjct: 604  RCVEEKYNARLETFEPSKSVAEKDYEHKE-LSEPSFVPSATDW-----------SKIAVK 663

Query: 672  IENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVDRANRVGTTLMDEIN 731
             E +T             EN+SA+GGSIW RRGKPT FPR+E  + R NR GT+LMDE++
Sbjct: 664  NEIRT-----------LHENVSAMGGSIWLRRGKPTGFPRVEIRMSRENRAGTSLMDEMS 723

Query: 732  HEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKANIEDSGNFSRSSK 791
             EITGDE V +TLLS L GEEEEE+FTPDKENFTPNTLLMKSLKKKA IE SG+  RSSK
Sbjct: 724  SEITGDETVTSTLLSDLGGEEEEEMFTPDKENFTPNTLLMKSLKKKATIESSGDSFRSSK 783

Query: 792  SQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKLAKQMSKNRTFGKEKIVIKKGGAE 851
            SQTSIFKSRH VKQEEELSEESDKENQT R LQEQKLAKQ+SKNR FGKEK++IK+GG E
Sbjct: 784  SQTSIFKSRHNVKQEEELSEESDKENQTSRALQEQKLAKQISKNRKFGKEKLLIKRGGTE 843

Query: 852  RAPFQSLQSNLAEKKRLEASIVKSARKSNISVSTGAMKNKFTEEGKKRWTMVVDINSLLN 911
            RAPFQSLQSNLAEKK  EA+   SARKSNI+VSTGAMK K T  GKKRWTMVVDINSLLN
Sbjct: 844  RAPFQSLQSNLAEKKSPEAT---SARKSNIAVSTGAMKKKLT-VGKKRWTMVVDINSLLN 903

Query: 912  KESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSLFRKTTDAASILQWIEDCIVKTRW 971
            KESMKSLQ LQGLQGTHLI+PR+VIRELDCLRRH  LFRKTT+AAS+L+WIEDC+VKTRW
Sbjct: 904  KESMKSLQRLQGLQGTHLIVPRIVIRELDCLRRHGRLFRKTTEAASVLEWIEDCMVKTRW 963

Query: 972  WIHVQNSGEGEPPATPPATPQSPCTEGSSQSLFWRTASSIQSI-TQRSFMEALSPTPEDH 1031
            WI V+   EG P ATPPATPQ+P TE SS+SL WRT+S IQSI  QRS MEALSPTPEDH
Sbjct: 964  WIDVECWEEGRPAATPPATPQAPYTEESSESLLWRTSSKIQSIAAQRSLMEALSPTPEDH 986

Query: 1032 ILDSALHF-RRGVKHGQLVLLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFLWAD 1091
            ILD ALH  RR V  GQLV+LSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFLWAD
Sbjct: 1024 ILDCALHARRRAVNRGQLVVLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFLWAD 986

Query: 1092 SSPRGLTWSCSDDVVLRERYDRCPSRSSKGAEGAKGLKLILLHNSHYGMLR 1140
            SSPRGLTWSC DDVVLRERYDRC SRSSKG EGAKGLKLILLHNSHYG  R
Sbjct: 1084 SSPRGLTWSCPDDVVLRERYDRCSSRSSKGLEGAKGLKLILLHNSHYGASR 986

BLAST of Lcy12g006250 vs. ExPASy TrEMBL
Match: A0A6J1EV89 (FHA domain-containing protein PS1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438377 PE=4 SV=1)

HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 738/1137 (64.91%), Postives = 824/1137 (72.47%), Query Frame = 0

Query: 12   QNEMKIPLFTVLKNGAILKNIFIVNNVADRENEEVIILGRHPDCNIMLTHPSISRFHLQI 71
            + E KIP+F+VLKNGAILKN+F+VNNV DRENEE I+LGRHPDCNIMLTHPSISRFHLQI
Sbjct: 4    REEEKIPVFSVLKNGAILKNLFLVNNVTDRENEETIVLGRHPDCNIMLTHPSISRFHLQI 63

Query: 72   HSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKLHWVPLSCA 131
            H NPSS K+ VVDLSSVHGTWVSGKRIEAG RVEMREGDTL VGGSSRVYKLHWVPL  A
Sbjct: 64   HLNPSSHKVFVVDLSSVHGTWVSGKRIEAGARVEMREGDTLSVGGSSRVYKLHWVPLRDA 123

Query: 132  YDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENIEPLYSDENWNMEM 191
            YDF+CPKE KEE++AII+E+AVEDCEK+ISL DENKETVEDSV ++I PLYSDE+WNME+
Sbjct: 124  YDFECPKENKEEELAIIDEKAVEDCEKEISLSDENKETVEDSVSDSIVPLYSDESWNMEL 183

Query: 192  -KEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPFGDELKSLEMSPQPP 251
             KEV +A P GEVEGMV+SSV          NEC KEE SL S PFG+ELKSLEM     
Sbjct: 184  IKEVSVAPPSGEVEGMVVSSV----------NECGKEETSLFSIPFGNELKSLEM----- 243

Query: 252  FLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIPLENVSRSSLSEKQQ 311
                                                                        
Sbjct: 244  ------------------------------------------------------------ 303

Query: 312  CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVFEREETSSIWTIRLEN 371
                  S Q P LPLSA+NLSFNVENIIMSS FD+ET+SSS N+FE EET+         
Sbjct: 304  ------SLQSPCLPLSADNLSFNVENIIMSSIFDSETESSSCNMFEWEETN--------- 363

Query: 372  KSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAMIAFAGGEC--- 431
             SNIL  VD IT TSENGYQQLDNE +S QP S  +CK + SD+EDT++   A  E    
Sbjct: 364  -SNILPVVDVIT-TSENGYQQLDNEKESLQPPSMSVCKGNVSDIEDTSIEMRAHLEMSLN 423

Query: 432  --DSSSINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNENGQLLAEKNF 491
              +SSS NEFEQ EI            SNVA+VD+EK       Q FD E+ Q+LAE+N 
Sbjct: 424  IYESSSRNEFEQIEI------------SNVAVVDDEK------PQPFDIESRQVLAERNS 483

Query: 492  SSNAEVVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHE-LHPLEDDHSSVTT 551
            SSN   V EIQ + P  EVK           S+T TEK EFM E E LHPLED+HSS++T
Sbjct: 484  SSNVGDVVEIQYVAPDQEVK-----------SETATEKNEFMEERELLHPLEDNHSSIST 543

Query: 552  EKGIQEMEILNKSQAEVGEDCVDKGNEELYQVTECLLDCQSQYKNDSSMKTTSEVTPNST 611
            E+ +Q+ME+L+KSQAE+GED VDK NEEL                               
Sbjct: 544  EQCLQDMEMLSKSQAEIGEDYVDKRNEEL------------------------------- 603

Query: 612  MNQNVEYCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEPSFVPCATEFVYSSLPDEEVS 671
                        Y+  LET EPS+SV EKDY HKE LSEPSF+PCAT+ V       EV 
Sbjct: 604  ------------YTARLETFEPSESVAEKDYEHKE-LSEPSFLPCATDLV----DVVEVC 663

Query: 672  PKIDVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVDRANRVGTT 731
             KI V+ E QT  ENV+ T+P RSEN+SA+GGSIW RRG+PT FPR+E    R NR GT+
Sbjct: 664  SKIAVKKEIQTLHENVEATSPTRSENVSAMGGSIWLRRGRPTGFPRVEIGASRENRAGTS 723

Query: 732  LMDEINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKANIEDSGN 791
            LMDE++ EITGDE V NTLLS L GEEEEE+FTPDKENFTPNTLLMKSL KKA IE SG+
Sbjct: 724  LMDEMSSEITGDETVTNTLLSDLGGEEEEEMFTPDKENFTPNTLLMKSLNKKATIESSGD 783

Query: 792  FSRSSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKLAKQMSKNRTFGKEKIVI 851
              RSSKSQTS+FKSRH VKQEEELSEESDKENQTPR LQEQKLAKQ+SKNR FGKEK++I
Sbjct: 784  SFRSSKSQTSMFKSRHNVKQEEELSEESDKENQTPRALQEQKLAKQISKNRKFGKEKLLI 843

Query: 852  KKGGAERAPFQSLQSNLAEKKRLEASIVKSARKSNISVSTGAMKNKFTEEGKKRWTMVVD 911
            K+GG ERAPFQSLQ NLAEKKR EA+   SARKSNI++STGAMK K T EGKKRWTMVVD
Sbjct: 844  KRGGMERAPFQSLQLNLAEKKRPEAT---SARKSNIAMSTGAMKKKLTVEGKKRWTMVVD 903

Query: 912  INSLLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSLFRKTTDAASILQWIEDC 971
            INSLLNKESMKSLQ LQGLQGTHLI+PR+VIRELDCLRRH  LFRKTT+AAS+L+WIEDC
Sbjct: 904  INSLLNKESMKSLQRLQGLQGTHLIVPRIVIRELDCLRRHGRLFRKTTEAASVLEWIEDC 963

Query: 972  IVKTRWWIHVQNSGEGEPPATPPATPQSPCTEGSSQSLFWRTASSIQSI-TQRSFMEALS 1031
            +VKTRWWI V++  EG P ATPPATPQ+P TE SS+SL WRT+S IQSI  QRS MEALS
Sbjct: 964  MVKTRWWIDVESWEEGRPAATPPATPQAPYTEESSESLLWRTSSKIQSIAAQRSLMEALS 968

Query: 1032 PTPEDHILDSALHF-RRGVKHGQLVLLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSE 1091
            PTPEDHILD ALH  RR V HGQLV+LSDDVTLKIKAMAEGLICETAKEFRESLVNPFSE
Sbjct: 1024 PTPEDHILDCALHARRRAVNHGQLVVLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSE 968

Query: 1092 RFLWADSSPRGLTWSCSDDVVLRERYDRCPSRSSKGAEGAKGLKLILLHNSHYGMLR 1140
            RFLWADSSPRGLTWSC DDVVLRERYDRC SRSSKG+EGAKGLKLILLHNSHYG+ R
Sbjct: 1084 RFLWADSSPRGLTWSCPDDVVLRERYDRCSSRSSKGSEGAKGLKLILLHNSHYGVSR 968

BLAST of Lcy12g006250 vs. ExPASy TrEMBL
Match: A0A0A0K684 (FHA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G290470 PE=4 SV=1)

HSP 1 Score: 1257.7 bits (3253), Expect = 0.0e+00
Identity = 721/1143 (63.08%), Postives = 810/1143 (70.87%), Query Frame = 0

Query: 1    MADREGQSNPDQNEMKIPLFTVLKNGAILKNIFIVNNVADRENEEVIILGRHPDCNIMLT 60
            M  RE +  PD+NE+K+P+FTVLKNGA LKNIFIVNNVADRE EEVI LGRHPDCNIMLT
Sbjct: 1    MPGRE-EETPDRNELKVPVFTVLKNGATLKNIFIVNNVADREKEEVITLGRHPDCNIMLT 60

Query: 61   HPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRV 120
            HPSISRFHLQIHSNPSSQK+ VVDLSSVHGTWVSGKRIE G+ VEM+EGDTLRVGGSSRV
Sbjct: 61   HPSISRFHLQIHSNPSSQKIFVVDLSSVHGTWVSGKRIETGDEVEMKEGDTLRVGGSSRV 120

Query: 121  YKLHWVPLSCAYDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENIEP 180
            Y+LHWVPLSCAYDF+ PKE KE +VAI+EE+ V+DCEK+ISLLDENKE   DSV ++IEP
Sbjct: 121  YRLHWVPLSCAYDFEGPKEMKEHEVAIVEEKDVKDCEKEISLLDENKERAVDSVFDSIEP 180

Query: 181  LYSDENWNME-MKEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPFGDE 240
            LY DENWN E MKEVPLA PL EV+ M +  V+  ESV+DLR EC++ E SLLSKPFG+E
Sbjct: 181  LYPDENWNTEMMKEVPLAPPLSEVKEMAVPLVNRVESVSDLRIECEQVETSLLSKPFGNE 240

Query: 241  LKSLEMSPQPPFLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIPLEN 300
            LK LEMS QPP LPLSAENLSFNVE+IIMSSFFG+++K SSSS FE ND+S IWNIP+EN
Sbjct: 241  LKGLEMSLQPPSLPLSAENLSFNVENIIMSSFFGNDNKISSSSMFEWNDTSGIWNIPMEN 300

Query: 301  VSRSSLSEKQQCQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVFEREE 360
            +S +SL  +Q C SK ESPQ PSL LSAENLSFNVENIIMSSFFD E+KSSS N+     
Sbjct: 301  ISSNSLYGRQLCHSKTESPQQPSLHLSAENLSFNVENIIMSSFFDGESKSSSCNM----- 360

Query: 361  TSSIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAM 420
                    LENKSNILS VDD  AT +                 ++LC+           
Sbjct: 361  ------PALENKSNILSVVDDTEATPD-------------YATFNILCQ----------- 420

Query: 421  IAFAGGECDSSSINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNENGQL 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  LAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHELHPLEDDH 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SSVTTEKGIQEMEILNKSQAEVGEDCVDKGNEELYQVTECLLDCQSQYKNDSSMKTTSEV 600
                                               QVTE  LDC  + KNDS +K+TSEV
Sbjct: 541  -----------------------------------QVTESFLDCLLEPKNDSPVKSTSEV 600

Query: 601  TPNSTMNQNVEYCIEEKYSTSLETIEPSK-SVPEKDYVHKEQLSEPSFVPCATEFVYSSL 660
            T NS M  NVE C+EE Y+  LE +EPSK S+P +D  H+E+LSE SF+ CA E+VYSSL
Sbjct: 601  TSNSPMTHNVECCVEETYNGRLEMLEPSKSSIPGEDNEHREELSESSFISCALEYVYSSL 660

Query: 661  PDEEVSPKIDVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVDRA 720
            PDEEV P+I V+ E QTP EN+D+T PIRSE+ SA+GG+I  R+GKPTSFP+IET V + 
Sbjct: 661  PDEEVPPEIAVEKECQTPHENLDLTLPIRSESASAMGGNISLRKGKPTSFPQIETGVSQT 720

Query: 721  NRVGTTLMDEINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKAN 780
            NR GT L DE NHEI G++   NT L+HLD +EEEEIFTPDKENFTPNTLLMKSLKKKA+
Sbjct: 721  NRAGTLLTDEFNHEIVGEKSGTNT-LAHLD-DEEEEIFTPDKENFTPNTLLMKSLKKKAS 780

Query: 781  IEDSGNFSRSSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKLAKQMSKNRTFG 840
            IEDSGN  RSSKSQTSIFKSRHK+K EEELSEESDKEN+TPRVLQEQKL+KQ  + R   
Sbjct: 781  IEDSGNCFRSSKSQTSIFKSRHKIKLEEELSEESDKENRTPRVLQEQKLSKQFCQRR-LE 840

Query: 841  KEKIVIKKGGAERAPFQSLQSNLAEKKRLEASIV-KSARKSNISVSTGAMKNKFTEEGKK 900
            +E  + KKGG  RAPFQSLQSN+A KKRLEA++V KSARKSN SV TGA+KNKFT E KK
Sbjct: 841  QENTMTKKGGGGRAPFQSLQSNVAGKKRLEAALVKKSARKSNTSVCTGAVKNKFTVEEKK 900

Query: 901  RWTMVVDINSLLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSLFRKTTDAASI 960
             WTMVVD +SLL+KESMKSLQLLQGLQGT LI+PR+VIRELD LRRH SLFRK T+AASI
Sbjct: 901  CWTMVVDTDSLLSKESMKSLQLLQGLQGTQLIVPRIVIRELDSLRRHGSLFRKRTEAASI 948

Query: 961  LQWIEDCIVKTRWWIHVQNSGEGEPPATPPATPQSPCTEGSSQSLFWRTASSIQSITQRS 1020
            LQWIEDC+V+TRWWIHVQ+S EG  P TPPATPQSP TEG SQSLFWRT SSIQSI QRS
Sbjct: 961  LQWIEDCMVQTRWWIHVQSSEEGVAPVTPPATPQSPYTEGRSQSLFWRT-SSIQSIKQRS 948

Query: 1021 FMEALSPTPEDHILDSALHFRRGVKHGQ-LVLLSDDVTLKIKAMAEGLICETAKEFRESL 1080
            FMEALSPTPEDHILD AL+FRRGVKHGQ LVL+SDDVTLKIK+MAEGLICETAKEFRESL
Sbjct: 1021 FMEALSPTPEDHILDCALYFRRGVKHGQELVLISDDVTLKIKSMAEGLICETAKEFRESL 948

Query: 1081 VNPFSERFLWADSSPRGLTWSCSDDVVLRERYDRCPSRSSKGAEGAKGLKLILLHNSHYG 1140
            VNPFSERFLWA+SSPRGLTWSC DD+VLRERYDRC SRSSKGAEGAKGLKLILLHNSHYG
Sbjct: 1081 VNPFSERFLWAESSPRGLTWSCPDDIVLRERYDRCWSRSSKGAEGAKGLKLILLHNSHYG 948

BLAST of Lcy12g006250 vs. NCBI nr
Match: XP_038897274.1 (FHA domain-containing protein PS1 [Benincasa hispida])

HSP 1 Score: 1595.5 bits (4130), Expect = 0.0e+00
Identity = 875/1144 (76.49%), Postives = 969/1144 (84.70%), Query Frame = 0

Query: 1    MADREGQSNPDQNEMKIPLFTVLKNGAILKNIFIVNNVADRENEEVIILGRHPDCNIMLT 60
            MADRE +  PDQ EMKIP+FTVLKNGAILKNIFIVNNV DR NEEVI LGRHPDCNIMLT
Sbjct: 1    MADRE-EETPDQTEMKIPVFTVLKNGAILKNIFIVNNVGDRVNEEVITLGRHPDCNIMLT 60

Query: 61   HPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRV 120
            HPSISRFHLQIHSN SSQKLCVVDLSSVHGT +SGKRIE G+RVEMREGDTLRVGGSSRV
Sbjct: 61   HPSISRFHLQIHSNLSSQKLCVVDLSSVHGTCISGKRIETGDRVEMREGDTLRVGGSSRV 120

Query: 121  YKLHWVPLSCAYDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENIEP 180
            Y+LHWVPLSCAYDF+ PKEKKE +VAI+EE+AV+DCEK+ISLLDE KETV DSV  +IEP
Sbjct: 121  YRLHWVPLSCAYDFEGPKEKKEHEVAIVEEKAVQDCEKEISLLDETKETVVDSVFGSIEP 180

Query: 181  LYSDENWNME-MKEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPFGDE 240
            LYSDENWNME MKE PLA PL EVE  V SSVD  +  +DLR EC++ E SLLS PFG+E
Sbjct: 181  LYSDENWNMEMMKEAPLAPPLSEVEEAV-SSVDHVKCASDLRTECEQVETSLLSIPFGNE 240

Query: 241  LKSLEMSPQPPFLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIPLEN 300
            +  LEM+ QPP L LSAEN  FNVE+IIMSSFFGS+S+SSSSST   ND+SSIWNIPLEN
Sbjct: 241  MNGLEMTFQPPSLSLSAENFPFNVENIIMSSFFGSDSQSSSSST---NDTSSIWNIPLEN 300

Query: 301  VSRSSLSEKQQCQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVFEREE 360
            +S SS   KQQCQ+KIESPQ PSLPLSAENLSFNVENIIMSSFFD E+K+SS N+FE +E
Sbjct: 301  IS-SSQYGKQQCQTKIESPQQPSLPLSAENLSFNVENIIMSSFFDGESKNSSCNMFEWKE 360

Query: 361  TSSIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAM 420
            T +I TI LEN SNILS VDDI AT E  YQ L +ENKS +  S  L KE+SSDV+DTAM
Sbjct: 361  TGNILTIPLENNSNILSVVDDIEATPEYEYQLLVDENKSLRLHSMSLYKENSSDVQDTAM 420

Query: 421  IAFAGGECDSSSINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNENGQL 480
             AF+  E  S S N+FEQKEI+N+MT+PLGHELS+V +VD EK I D+E +QF       
Sbjct: 421  TAFSDVEWGSPSRNKFEQKEISNIMTMPLGHELSHVTVVDNEKPISDNESRQF------- 480

Query: 481  LAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHELHPLEDDH 540
            +AE+N SSN   V+E Q+L PVHEVKSSSTQP LALQS+TVTEK EF+ EHELHPL+D+H
Sbjct: 481  VAERNLSSNFADVDETQDL-PVHEVKSSSTQPLLALQSETVTEKNEFIAEHELHPLQDNH 540

Query: 541  SSVTTEKGIQEMEILNKSQAEVGEDCVDKGNEELY-QVTECLLDCQSQYKNDSSMKTTSE 600
            SS++ EKGIQE+E+  K QAE GE  VDKGN ELY Q+TECLLDC S+YKNDSS+K T E
Sbjct: 541  SSISKEKGIQEIEMHTKIQAEDGEGYVDKGNGELYHQITECLLDCLSEYKNDSSVKATFE 600

Query: 601  VTPNSTMNQNVEYCIEEKYSTSLETIEPSK-SVPEKDYVHKEQLSEPSFVPCATEFVYSS 660
            +T NS M QNVE CIEE Y+  L+T+EPSK S+   DY HKE LSEPSF+ CA E+VYSS
Sbjct: 601  ITTNSLMKQNVECCIEETYNARLKTLEPSKSSMAWGDYEHKE-LSEPSFISCALEYVYSS 660

Query: 661  LPDEEVSPKIDVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVDR 720
            LP EEV P+I V+ E QTP  N DVT PIRSE+ SA+GG+IW RRGKPTSFPRIET + R
Sbjct: 661  LPGEEVPPEIAVEKECQTPHGNSDVTLPIRSESASAMGGNIWLRRGKPTSFPRIETGLIR 720

Query: 721  ANRVGTTLMDEINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKA 780
             NR GT+LMDEINHEI GD+ V NT LSHL  EEEEE+FTPDKENFTPNTLLMKSLKKKA
Sbjct: 721  TNRAGTSLMDEINHEIAGDKSVTNT-LSHLGEEEEEEMFTPDKENFTPNTLLMKSLKKKA 780

Query: 781  NIEDSGNFSRSSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKLAKQMSKNRTF 840
            +IEDSGN  RSSKSQTSIFKSRHKVK EEELSEESDKENQTPRVLQEQKL+KQ+SKNR F
Sbjct: 781  SIEDSGNCFRSSKSQTSIFKSRHKVKLEEELSEESDKENQTPRVLQEQKLSKQISKNRRF 840

Query: 841  GKEKIVIKKGGAERAPFQSLQSNLAEKKRLEASIV-KSARKSNISVSTGAMKNKFTEEGK 900
            G+EK + KKGG ER PFQSLQSN+A KKRLEA++V KSARKSNI V TGAMKNKFT EGK
Sbjct: 841  GQEKTMTKKGGVERTPFQSLQSNIAGKKRLEATVVKKSARKSNIYVCTGAMKNKFTVEGK 900

Query: 901  KRWTMVVDINSLLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSLFRKTTDAAS 960
            KRWTMVVD NSLLNKESMKSLQLL+GLQGTHLIIPR+VI+ELD L+RH+SLFRKTT+AAS
Sbjct: 901  KRWTMVVDTNSLLNKESMKSLQLLEGLQGTHLIIPRIVIKELDSLKRHASLFRKTTEAAS 960

Query: 961  ILQWIEDCIVKTRWWIHVQNSGEGEPPATPPATPQSPCTEGSSQSLFWRTASSIQSITQR 1020
            +LQWIEDC+V+TRWWIHVQ+  EG PP TPPATPQSP TEGSSQSLFWRT SSIQS TQR
Sbjct: 961  VLQWIEDCMVQTRWWIHVQSLEEGVPPVTPPATPQSPYTEGSSQSLFWRTTSSIQSTTQR 1020

Query: 1021 SFMEALSPTPEDHILDSALHF-RRGVKHGQLVLLSDDVTLKIKAMAEGLICETAKEFRES 1080
              MEA SPTPE HILD ALHF RRGV HGQLVL+SDDVTLKIKAMAEGLICETAKEFRES
Sbjct: 1021 -VMEAPSPTPEGHILDCALHFRRRGVNHGQLVLISDDVTLKIKAMAEGLICETAKEFRES 1080

Query: 1081 LVNPFSERFLWADSSPRGLTWSCSDDVVLRERYDRCPSRSSKGAEGAKGLKLILLHNSHY 1140
            LVNPFSERFLWADSSPRGLTWSC DD+VLRERYDRC  RSSKGAEGAKGLKLILLHNSHY
Sbjct: 1081 LVNPFSERFLWADSSPRGLTWSCPDDIVLRERYDRCSLRSSKGAEGAKGLKLILLHNSHY 1127

BLAST of Lcy12g006250 vs. NCBI nr
Match: XP_022135216.1 (FHA domain-containing protein PS1 isoform X2 [Momordica charantia])

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 817/1154 (70.80%), Postives = 909/1154 (78.77%), Query Frame = 0

Query: 1    MADR-EGQSNPDQNEMKIPLFTVLKNGAILKNIFIVNNVADREN-EEVIILGRHPDCNIM 60
            MADR E +S PD N+ KIP+FTVLKNGAILKNIFI+NN  +  N EEVIILGRHPDCNIM
Sbjct: 1    MADREEEESKPDLNDKKIPVFTVLKNGAILKNIFILNNFPNPGNEEEVIILGRHPDCNIM 60

Query: 61   LTHPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSS 120
            LTHPSISRFHLQIHSNPSS KLC+VDLSSVHGTWVSGK+IEAG RVEMREG+TLRVGGSS
Sbjct: 61   LTHPSISRFHLQIHSNPSSLKLCLVDLSSVHGTWVSGKKIEAGARVEMREGNTLRVGGSS 120

Query: 121  RVYKLHWVPLSCAYDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENI 180
            R+Y+LHWVPLS  YDF+   EKKEE+VAI EE AVE+CEK+ SLLDE KETV+D    NI
Sbjct: 121  RIYRLHWVPLSRVYDFEI--EKKEEEVAIAEERAVENCEKENSLLDEKKETVKDLFFGNI 180

Query: 181  -EPLYSDENWNME-MKEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPF 240
             EPLYSDE+W++E MKEV  A P+GE EGMV+S VDGGES + LRNEC +EE  LLSKPF
Sbjct: 181  VEPLYSDEDWSIEMMKEVTSAPPMGEFEGMVVSPVDGGESESHLRNECPQEETCLLSKPF 240

Query: 241  GDELKSLEMSPQPPFLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIP 300
            G+E KSL       F PLSAENLSFNVE+IIMSSFFGSE+KS          +SSIW +P
Sbjct: 241  GNESKSL-------FAPLSAENLSFNVENIIMSSFFGSENKS----------TSSIWTMP 300

Query: 301  LENVSRSSLSEKQQ-CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVF 360
            LEN SR S S  QQ      ESPQ  SLPLSAENLSFNVENIIMSSFF +E+KSSS+N F
Sbjct: 301  LENESRISSSGNQQLLHHGKESPQSVSLPLSAENLSFNVENIIMSSFFSSESKSSSSNTF 360

Query: 361  EREETSSIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVE 420
            ++EET   WTI LENKS  LS VDD      NGY+Q DNEN+SPQ  S  LCKE+SS+VE
Sbjct: 361  DQEETDRSWTIPLENKSKNLSVVDDRKPAPANGYRQFDNENQSPQTHSVPLCKENSSNVE 420

Query: 421  DTAMIAFAGGECDSSSINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNE 480
            D  M               FEQKEI+++MTIPLGHEL++ A+ D+ K I  HE Q FD E
Sbjct: 421  DNDM---------------FEQKEISSIMTIPLGHELTDEAVADDAKPI-SHESQNFDLE 480

Query: 481  N----GQLLAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHE 540
            N      LL+ KN SS+ E     QNL  + EV S        ++  T+TEK+E MVEHE
Sbjct: 481  NESPQSVLLSGKNLSSDVET----QNL-SICEVNSLIYDVGNVVE--TITEKEELMVEHE 540

Query: 541  LHPLEDDHSSVTTEKGIQEMEILNKSQAEVGEDCVDKGNEEL---YQVTECLLDCQSQYK 600
            LHPLE +HSSV+TE+ I E E L+K + EV E  VD+GN +    YQV E LL+ QSQY 
Sbjct: 541  LHPLEYNHSSVSTEERIHETETLDKIETEVEEVNVDEGNGDFYHTYQVKESLLNWQSQYI 600

Query: 601  NDSSMKTTSEVTPNSTMNQNVEYCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEPSFVP 660
            N++SMK+T EV PN  MNQNV  C+EEK +   ET++P+KSV EKD+  KE LSEP  V 
Sbjct: 601  NETSMKSTPEVIPNLPMNQNVGCCVEEKDTAGYETLKPTKSVQEKDHEQKE-LSEPGTVS 660

Query: 661  CATEFVYSSLPDEEVSPKIDVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSF 720
             + EFV SSLPDE+VS +I V+ E QTP          RS+N+SA+G SIW RRGKP SF
Sbjct: 661  SSMEFVNSSLPDEDVSLEISVEKEFQTP----------RSDNVSAMGDSIWLRRGKPNSF 720

Query: 721  PRIETDVDRANRVGTTLMDEINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTL 780
            PRIET V+RANRVGT+L DEINHEI  DE V NTLL  L GEEEEEIFTPDKENFTPNTL
Sbjct: 721  PRIETGVNRANRVGTSLTDEINHEIAEDETVVNTLLPIL-GEEEEEIFTPDKENFTPNTL 780

Query: 781  LMKSLKKKANIEDS-GNFSRSSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKL 840
            LMKSLKKKANIEDS  N SR SKSQ+SIFKSR K+K  EELSEESDKENQTPR LQEQKL
Sbjct: 781  LMKSLKKKANIEDSVKNSSRPSKSQSSIFKSRQKIK-PEELSEESDKENQTPRFLQEQKL 840

Query: 841  AKQMSKNRTFGKEKIVIKKGGAERAPFQSLQSNLAEKKRLEASIVKSARKSNISVSTGAM 900
            AK +SKNR FGK+K V+K+GG ERAPFQSLQSNLA KKR E ++V SARKSNISV TGAM
Sbjct: 841  AKSISKNRRFGKDKKVMKRGGGERAPFQSLQSNLAGKKRPEVTVVNSARKSNISVCTGAM 900

Query: 901  KNKFTEEGKKRWTMVVDINSLLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSL 960
            KNKFT EGKKRWTMVVDINSLLNKESMKSL+LLQGLQGTHLI+PRMVIRELDCLRRH SL
Sbjct: 901  KNKFT-EGKKRWTMVVDINSLLNKESMKSLKLLQGLQGTHLIVPRMVIRELDCLRRHGSL 960

Query: 961  FRKTTDAASILQWIEDCIVKTRWWIHVQNSGE-GEPPATPPATPQSPCTEGSSQSLFWRT 1020
            FRKTT+AASILQWIEDC+V+TRWWIHVQ+S E G PPAT PATP+SP TEG SQSL WRT
Sbjct: 961  FRKTTEAASILQWIEDCMVQTRWWIHVQSSDEGGRPPATSPATPRSPYTEG-SQSLLWRT 1020

Query: 1021 ASSIQSITQRSFMEALSPTPEDHILDSALHFRRGVKHGQLVLLSDDVTLKIKAMAEGLIC 1080
            ASSIQSITQRSFME LSPT EDHIL+ ALHFRRGV HGQLVLLSDDVTLKIKAMAEGLIC
Sbjct: 1021 ASSIQSITQRSFMEVLSPTAEDHILECALHFRRGVNHGQLVLLSDDVTLKIKAMAEGLIC 1080

Query: 1081 ETAKEFRESLVNPFSERFLWADSSPRGLTWSCSDDVVLRERYDRCPSRSSKG-AEGAKGL 1140
            ETAKEFRESLVNPFSERFLWADSSPRGLTWSC DDVVLRERYDRC S S KG AEGAKGL
Sbjct: 1081 ETAKEFRESLVNPFSERFLWADSSPRGLTWSCPDDVVLRERYDRCSSWSLKGTAEGAKGL 1097

BLAST of Lcy12g006250 vs. NCBI nr
Match: XP_022135215.1 (FHA domain-containing protein PS1 isoform X1 [Momordica charantia])

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 817/1158 (70.55%), Postives = 909/1158 (78.50%), Query Frame = 0

Query: 1    MADR-EGQSNPDQNEMKIPLFTVLKNGAILKNIFIVNNVADREN-EEVIILGRHPDCNIM 60
            MADR E +S PD N+ KIP+FTVLKNGAILKNIFI+NN  +  N EEVIILGRHPDCNIM
Sbjct: 1    MADREEEESKPDLNDKKIPVFTVLKNGAILKNIFILNNFPNPGNEEEVIILGRHPDCNIM 60

Query: 61   LTHPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSS 120
            LTHPSISRFHLQIHSNPSS KLC+VDLSSVHGTWVSGK+IEAG RVEMREG+TLRVGGSS
Sbjct: 61   LTHPSISRFHLQIHSNPSSLKLCLVDLSSVHGTWVSGKKIEAGARVEMREGNTLRVGGSS 120

Query: 121  RVYKLHWVPLSCAYDFQCPKEKKEEKVAIIEEEAVEDCE----KDISLLDENKETVEDSV 180
            R+Y+LHWVPLS  YDF+   EKKEE+VAI EE AVE+CE    K+ SLLDE KETV+D  
Sbjct: 121  RIYRLHWVPLSRVYDFEI--EKKEEEVAIAEERAVENCEIVLQKENSLLDEKKETVKDLF 180

Query: 181  LENI-EPLYSDENWNME-MKEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLL 240
              NI EPLYSDE+W++E MKEV  A P+GE EGMV+S VDGGES + LRNEC +EE  LL
Sbjct: 181  FGNIVEPLYSDEDWSIEMMKEVTSAPPMGEFEGMVVSPVDGGESESHLRNECPQEETCLL 240

Query: 241  SKPFGDELKSLEMSPQPPFLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSI 300
            SKPFG+E KSL       F PLSAENLSFNVE+IIMSSFFGSE+KS          +SSI
Sbjct: 241  SKPFGNESKSL-------FAPLSAENLSFNVENIIMSSFFGSENKS----------TSSI 300

Query: 301  WNIPLENVSRSSLSEKQQ-CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSS 360
            W +PLEN SR S S  QQ      ESPQ  SLPLSAENLSFNVENIIMSSFF +E+KSSS
Sbjct: 301  WTMPLENESRISSSGNQQLLHHGKESPQSVSLPLSAENLSFNVENIIMSSFFSSESKSSS 360

Query: 361  ANVFEREETSSIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESS 420
            +N F++EET   WTI LENKS  LS VDD      NGY+Q DNEN+SPQ  S  LCKE+S
Sbjct: 361  SNTFDQEETDRSWTIPLENKSKNLSVVDDRKPAPANGYRQFDNENQSPQTHSVPLCKENS 420

Query: 421  SDVEDTAMIAFAGGECDSSSINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQ 480
            S+VED  M               FEQKEI+++MTIPLGHEL++ A+ D+ K I  HE Q 
Sbjct: 421  SNVEDNDM---------------FEQKEISSIMTIPLGHELTDEAVADDAKPI-SHESQN 480

Query: 481  FDNEN----GQLLAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFM 540
            FD EN      LL+ KN SS+ E     QNL  + EV S        ++  T+TEK+E M
Sbjct: 481  FDLENESPQSVLLSGKNLSSDVET----QNL-SICEVNSLIYDVGNVVE--TITEKEELM 540

Query: 541  VEHELHPLEDDHSSVTTEKGIQEMEILNKSQAEVGEDCVDKGNEEL---YQVTECLLDCQ 600
            VEHELHPLE +HSSV+TE+ I E E L+K + EV E  VD+GN +    YQV E LL+ Q
Sbjct: 541  VEHELHPLEYNHSSVSTEERIHETETLDKIETEVEEVNVDEGNGDFYHTYQVKESLLNWQ 600

Query: 601  SQYKNDSSMKTTSEVTPNSTMNQNVEYCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEP 660
            SQY N++SMK+T EV PN  MNQNV  C+EEK +   ET++P+KSV EKD+  KE LSEP
Sbjct: 601  SQYINETSMKSTPEVIPNLPMNQNVGCCVEEKDTAGYETLKPTKSVQEKDHEQKE-LSEP 660

Query: 661  SFVPCATEFVYSSLPDEEVSPKIDVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGK 720
              V  + EFV SSLPDE+VS +I V+ E QTP          RS+N+SA+G SIW RRGK
Sbjct: 661  GTVSSSMEFVNSSLPDEDVSLEISVEKEFQTP----------RSDNVSAMGDSIWLRRGK 720

Query: 721  PTSFPRIETDVDRANRVGTTLMDEINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFT 780
            P SFPRIET V+RANRVGT+L DEINHEI  DE V NTLL  L GEEEEEIFTPDKENFT
Sbjct: 721  PNSFPRIETGVNRANRVGTSLTDEINHEIAEDETVVNTLLPIL-GEEEEEIFTPDKENFT 780

Query: 781  PNTLLMKSLKKKANIEDS-GNFSRSSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQ 840
            PNTLLMKSLKKKANIEDS  N SR SKSQ+SIFKSR K+K  EELSEESDKENQTPR LQ
Sbjct: 781  PNTLLMKSLKKKANIEDSVKNSSRPSKSQSSIFKSRQKIK-PEELSEESDKENQTPRFLQ 840

Query: 841  EQKLAKQMSKNRTFGKEKIVIKKGGAERAPFQSLQSNLAEKKRLEASIVKSARKSNISVS 900
            EQKLAK +SKNR FGK+K V+K+GG ERAPFQSLQSNLA KKR E ++V SARKSNISV 
Sbjct: 841  EQKLAKSISKNRRFGKDKKVMKRGGGERAPFQSLQSNLAGKKRPEVTVVNSARKSNISVC 900

Query: 901  TGAMKNKFTEEGKKRWTMVVDINSLLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRR 960
            TGAMKNKFT EGKKRWTMVVDINSLLNKESMKSL+LLQGLQGTHLI+PRMVIRELDCLRR
Sbjct: 901  TGAMKNKFT-EGKKRWTMVVDINSLLNKESMKSLKLLQGLQGTHLIVPRMVIRELDCLRR 960

Query: 961  HSSLFRKTTDAASILQWIEDCIVKTRWWIHVQNSGE-GEPPATPPATPQSPCTEGSSQSL 1020
            H SLFRKTT+AASILQWIEDC+V+TRWWIHVQ+S E G PPAT PATP+SP TEG SQSL
Sbjct: 961  HGSLFRKTTEAASILQWIEDCMVQTRWWIHVQSSDEGGRPPATSPATPRSPYTEG-SQSL 1020

Query: 1021 FWRTASSIQSITQRSFMEALSPTPEDHILDSALHFRRGVKHGQLVLLSDDVTLKIKAMAE 1080
             WRTASSIQSITQRSFME LSPT EDHIL+ ALHFRRGV HGQLVLLSDDVTLKIKAMAE
Sbjct: 1021 LWRTASSIQSITQRSFMEVLSPTAEDHILECALHFRRGVNHGQLVLLSDDVTLKIKAMAE 1080

Query: 1081 GLICETAKEFRESLVNPFSERFLWADSSPRGLTWSCSDDVVLRERYDRCPSRSSKG-AEG 1140
            GLICETAKEFRESLVNPFSERFLWADSSPRGLTWSC DDVVLRERYDRC S S KG AEG
Sbjct: 1081 GLICETAKEFRESLVNPFSERFLWADSSPRGLTWSCPDDVVLRERYDRCSSWSLKGTAEG 1101

BLAST of Lcy12g006250 vs. NCBI nr
Match: XP_023523475.1 (FHA domain-containing protein PS1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1388.6 bits (3593), Expect = 0.0e+00
Identity = 784/1131 (69.32%), Postives = 861/1131 (76.13%), Query Frame = 0

Query: 12   QNEMKIPLFTVLKNGAILKNIFIVNNVADRENEEVIILGRHPDCNIMLTHPSISRFHLQI 71
            + E KIP+FTVLKNGAILKN+F+VNNV DRENEE I+LGRHPDCNIMLTHPSISRFHLQI
Sbjct: 4    REEEKIPVFTVLKNGAILKNLFLVNNVTDRENEETIVLGRHPDCNIMLTHPSISRFHLQI 63

Query: 72   HSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKLHWVPLSCA 131
            H NPSS K+ VVDLSSVHGTWVSGKRIEAG RVEMREGDTL VGGSSRVYKLHWVPL  A
Sbjct: 64   HLNPSSHKVFVVDLSSVHGTWVSGKRIEAGARVEMREGDTLSVGGSSRVYKLHWVPLRDA 123

Query: 132  YDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENIEPLYSDENWNMEM 191
            YDF+CPKEKKEE++AIIEE+AVEDCEK+ISL DENKETVEDSV ++I PLYSDENWNME+
Sbjct: 124  YDFECPKEKKEEELAIIEEKAVEDCEKEISLSDENKETVEDSVSDSIGPLYSDENWNMEL 183

Query: 192  -KEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPFGDELKSLEMSPQPP 251
             KEV +A   GEVEGMV+SSV          NEC KEE SLLS PFG+ELKSLEM     
Sbjct: 184  IKEVSVAPLAGEVEGMVVSSV----------NECGKEETSLLSIPFGNELKSLEM----- 243

Query: 252  FLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIPLENVSRSSLSEKQQ 311
                                                                        
Sbjct: 244  ------------------------------------------------------------ 303

Query: 312  CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVFEREETSSIWTIRLEN 371
                  S Q P LPLSA+NLSFNVENIIMSS FD+ET+SSS N+FE EE           
Sbjct: 304  ------SLQSPCLPLSADNLSFNVENIIMSSIFDSETESSSCNMFEWEEI---------- 363

Query: 372  KSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAMIAFAGGECDSS 431
            KSNIL  VD IT TSENGYQQLDNE +S QP S   CK + SD+EDT MIAFAGG+ +SS
Sbjct: 364  KSNILPVVDVIT-TSENGYQQLDNEKESLQPPSMSPCKGNFSDIEDTDMIAFAGGKYESS 423

Query: 432  SINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNENGQLLAEKNFSSNAE 491
            S NEFEQ EI            SNVA+VD+EK       Q FD E+ Q+LAE+N SSN  
Sbjct: 424  SRNEFEQIEI------------SNVAVVDDEK------PQPFDIESRQVLAERNSSSNVG 483

Query: 492  VVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHELHPLEDDHSSVTTEKGIQE 551
               EIQ LPP  EVK           S+T TEK EFM EHELHPLED+HSS++TEK +QE
Sbjct: 484  YGVEIQYLPPDQEVK-----------SETATEKNEFMEEHELHPLEDNHSSISTEKCLQE 543

Query: 552  MEILNKSQAEVGEDCVDKGNEELYQVTECLLDCQSQYKNDSSMKTTSEVTPNSTMNQNVE 611
            +E+L+KSQAE+GED  D+ NEELY+VTECLL CQ+QYKNDSSMKTTSEVTPN   NQNVE
Sbjct: 544  VEMLSKSQAEIGEDYADERNEELYKVTECLLYCQAQYKNDSSMKTTSEVTPNLPTNQNVE 603

Query: 612  YCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEPSFVPCATEFVYSSLPDEEVSPKIDVQ 671
             C+EEKY+  LET EPSKSV EKDY HKE LSEPSFVP AT+             KI V+
Sbjct: 604  RCVEEKYNARLETFEPSKSVAEKDYEHKE-LSEPSFVPSATD-----------CSKIAVK 663

Query: 672  IENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVDRANRVGTTLMDEIN 731
             E +T  ENVD  +P RSEN+SA+GGSIW RRGKPT FPR+E  V R NR GT+LMDE++
Sbjct: 664  NEIRTLHENVDAASPTRSENVSAMGGSIWLRRGKPTGFPRVEIGVSRENRAGTSLMDEMS 723

Query: 732  HEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKANIEDSGNFSRSSK 791
             EITGDE V NTLLS L GEEEEE+FTPDKENFTPNTLL KSL KKA IE SG+  RSSK
Sbjct: 724  SEITGDETVTNTLLSDLGGEEEEEMFTPDKENFTPNTLLKKSLNKKATIESSGDSFRSSK 783

Query: 792  SQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKLAKQMSKNRTFGKEKIVIKKGGAE 851
            SQTSIFKSRH VKQEEELSEESDKENQTPR LQEQKLAKQ+SKNR FGKEK++IK+GG E
Sbjct: 784  SQTSIFKSRHNVKQEEELSEESDKENQTPRALQEQKLAKQISKNRKFGKEKLLIKRGGME 843

Query: 852  RAPFQSLQSNLAEKKRLEASIVKSARKSNISVSTGAMKNKFTEEGKKRWTMVVDINSLLN 911
            RAPFQSLQSNLAEKKR EA+   SARKSNI+VSTGAMK K T EGKKRWTMVVDINSLLN
Sbjct: 844  RAPFQSLQSNLAEKKRPEAT---SARKSNIAVSTGAMKKKLTVEGKKRWTMVVDINSLLN 903

Query: 912  KESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSLFRKTTDAASILQWIEDCIVKTRW 971
            KESMKSLQ LQGLQGTHLI+PR+VIRELDCLRRH  LFRKTT+AAS+L+WIEDC+VKTRW
Sbjct: 904  KESMKSLQRLQGLQGTHLIVPRIVIRELDCLRRHGRLFRKTTEAASVLEWIEDCMVKTRW 963

Query: 972  WIHVQNSGEGEPPATPPATPQSPCTEGSSQSLFWRTASSIQSI-TQRSFMEALSPTPEDH 1031
            WI V++  EG P ATPPATPQ+P TE SS+SL WRT+S IQSI  QRS MEALSPTPEDH
Sbjct: 964  WIDVESWEEGRPAATPPATPQAPYTEESSESLLWRTSSKIQSIAAQRSLMEALSPTPEDH 998

Query: 1032 ILDSALHF-RRGVKHGQLVLLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFLWAD 1091
            ILD ALH  RR V HGQLV+LSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFLWAD
Sbjct: 1024 ILDCALHARRRAVNHGQLVVLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFLWAD 998

Query: 1092 SSPRGLTWSCSDDVVLRERYDRCPSRSSKGAEGAKGLKLILLHNSHYGMLR 1140
            SSPRGLTWSC DDVVLRERYDRC SRSSKG+EGAKGLKLILLHNSHYG+ R
Sbjct: 1084 SSPRGLTWSCPDDVVLRERYDRCSSRSSKGSEGAKGLKLILLHNSHYGVSR 998

BLAST of Lcy12g006250 vs. NCBI nr
Match: KAG6607593.1 (FHA domain-containing protein PS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1372.1 bits (3550), Expect = 0.0e+00
Identity = 774/1134 (68.25%), Postives = 860/1134 (75.84%), Query Frame = 0

Query: 12   QNEMKIPLFTVLKNGAILKNIFIVNNVADRENEEVIILGRHPDCNIMLTHPSISRFHLQI 71
            + E KIP+FTVLKNGAILKN+F+VNNV DRENEE I+LGRHPDCNIMLTHPSISRFHLQI
Sbjct: 4    REEEKIPVFTVLKNGAILKNLFLVNNVTDRENEETIVLGRHPDCNIMLTHPSISRFHLQI 63

Query: 72   HSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKLHWVPLSCA 131
            H NPSS K+ VVDLSSVHGTWVSGKRIEAG RVEMREGDTL VGGSSRVYKLHWVPL  A
Sbjct: 64   HLNPSSHKVFVVDLSSVHGTWVSGKRIEAGARVEMREGDTLSVGGSSRVYKLHWVPLRDA 123

Query: 132  YDFQCPKEKKEEKVAIIEEEAVEDCEKDISLLDENKETVEDSVLENIEPLYSDENWNMEM 191
            YDF+CPKEKKEE++AIIEE+AVEDCEK+ISL DENKETVEDSV ++I PLYSDENWNME+
Sbjct: 124  YDFECPKEKKEEELAIIEEKAVEDCEKEISLSDENKETVEDSVSDSIVPLYSDENWNMEL 183

Query: 192  -KEVPLAAPLGEVEGMVMSSVDGGESVNDLRNECKKEEISLLSKPFGDELKSLEMSPQPP 251
             KEV +A P  EVE MV+SSV          NEC KEE +LLS PFG+ELK LEM     
Sbjct: 184  IKEVSVAPPSEEVEAMVVSSV----------NECGKEETNLLSIPFGNELKRLEM----- 243

Query: 252  FLPLSAENLSFNVESIIMSSFFGSESKSSSSSTFEQNDSSSIWNIPLENVSRSSLSEKQQ 311
                                                                        
Sbjct: 244  ------------------------------------------------------------ 303

Query: 312  CQSKIESPQPPSLPLSAENLSFNVENIIMSSFFDAETKSSSANVFEREETSSIWTIRLEN 371
                  S Q P LPLSA+NLSFNVENIIMSS FD+ET+SSS N+FE EET          
Sbjct: 304  ------SLQSPCLPLSADNLSFNVENIIMSSIFDSETESSSCNMFEWEET---------- 363

Query: 372  KSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAMIAFAGGECDSS 431
            KSNIL+ VD +T T ENGYQQLDNE +S QP S  +CK + SD+ DT+MIAFAGG+ +SS
Sbjct: 364  KSNILAVVDVMT-TPENGYQQLDNEKESLQPPSMSVCKGNVSDIIDTSMIAFAGGKYESS 423

Query: 432  SINEFEQKEINNVMTIPLGHELSNVALVDEEKLILDHERQQFDNENGQLLAEKNFSSNAE 491
            S NEFEQ EI            SN+ +VD+EK       Q FD E+ Q+LAE+N SSN  
Sbjct: 424  SRNEFEQIEI------------SNIGVVDDEK------PQPFDIESRQVLAERNSSSNVG 483

Query: 492  VVEEIQNLPPVHEVKSSSTQPPLALQSKTVTEKKEFMVEHE---LHPLEDDHSSVTTEKG 551
               EIQ LPP  +VK           S+T TEK EFM EHE   LHPLED+HSS++TE+ 
Sbjct: 484  DGVEIQYLPPDQKVK-----------SETSTEKNEFMEEHELLLLHPLEDNHSSISTEQC 543

Query: 552  IQEMEILNKSQAEVGEDCVDKGNEELYQVTECLLDCQSQYKNDSSMKTTSEVTPNSTMNQ 611
            +QEME+L+KSQAE+GED  ++ NEELY+VTECLLDCQ+QYKNDSSMKTTSEVTPN   NQ
Sbjct: 544  LQEMEMLSKSQAEIGEDYAEERNEELYKVTECLLDCQAQYKNDSSMKTTSEVTPNLPTNQ 603

Query: 612  NVEYCIEEKYSTSLETIEPSKSVPEKDYVHKEQLSEPSFVPCATEFVYSSLPDEEVSPKI 671
            NVE C+EEKY+  LET EPSKSV EKDY HKE LSEPSFVP AT+             KI
Sbjct: 604  NVERCVEEKYNARLETFEPSKSVAEKDYEHKE-LSEPSFVPSATD-----------CSKI 663

Query: 672  DVQIENQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVDRANRVGTTLMD 731
             V+ E +T  ENVD  +P RSEN+SA+GGSIW RRGKPT FPR+E  V R NR GT+LMD
Sbjct: 664  AVKNEIRTLHENVDAASPTRSENVSAMGGSIWLRRGKPTGFPRVEIGVSRENRAGTSLMD 723

Query: 732  EINHEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKANIEDSGNFSR 791
            E++ EITGDE V NTLLS L GEEEEE+FTPD ENFTPNTLLMKSL KK  IE SG+  R
Sbjct: 724  EMSSEITGDETVTNTLLSDLGGEEEEEMFTPDNENFTPNTLLMKSLNKKVTIESSGDSFR 783

Query: 792  SSKSQTSIFKSRHKVKQEEELSEESDKENQTPRVLQEQKLAKQMSKNRTFGKEKIVIKKG 851
            SSKSQTS+FKSRH VKQEEELSEESDKENQTPR LQEQKLAKQ+SKNR FGKEK++IK+G
Sbjct: 784  SSKSQTSMFKSRHDVKQEEELSEESDKENQTPRALQEQKLAKQISKNRKFGKEKLLIKRG 843

Query: 852  GAERAPFQSLQSNLAEKKRLEASIVKSARKSNISVSTGAMKNKFTEEGKKRWTMVVDINS 911
            G ERAPFQSLQSNLAEKKR EA+   SARKSNI+VSTGAMK K T EGKKRWTMVVDINS
Sbjct: 844  GMERAPFQSLQSNLAEKKRPEAT---SARKSNIAVSTGAMKKKLTVEGKKRWTMVVDINS 903

Query: 912  LLNKESMKSLQLLQGLQGTHLIIPRMVIRELDCLRRHSSLFRKTTDAASILQWIEDCIVK 971
            LLNKESMKSLQ LQGLQGTHLI+PR+VIRELDCLRRH  LFRKTT+AAS+L+WIEDC+VK
Sbjct: 904  LLNKESMKSLQRLQGLQGTHLIVPRIVIRELDCLRRHGRLFRKTTEAASVLEWIEDCMVK 963

Query: 972  TRWWIHVQNSGEGEPPATPPATPQSPCTEGSSQSLFWRTASSIQSI-TQRSFMEALSPTP 1031
            TRWWI V++  EG P ATPPATPQ+P TE SS+SL WRT+S IQSI  QRS MEALSPTP
Sbjct: 964  TRWWIDVESWEEGRPAATPPATPQAPYTEESSESLLWRTSSKIQSIAAQRSLMEALSPTP 1001

Query: 1032 EDHILDSALHF-RRGVKHGQLVLLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFL 1091
            EDHILD ALH  RR V HGQLV+LSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFL
Sbjct: 1024 EDHILDCALHARRRAVNHGQLVVLSDDVTLKIKAMAEGLICETAKEFRESLVNPFSERFL 1001

Query: 1092 WADSSPRGLTWSCSDDVVLRERYDRCPSRSSKGAEGAKGLKLILLHNSHYGMLR 1140
            WADSSPRGLTWSC DDVVLRERYDRC SRSSKG+EGAKGLKLILLHNSHYG+ R
Sbjct: 1084 WADSSPRGLTWSCPDDVVLRERYDRCSSRSSKGSEGAKGLKLILLHNSHYGVSR 1001

BLAST of Lcy12g006250 vs. TAIR 10
Match: AT1G34355.1 (forkhead-associated (FHA) domain-containing protein )

HSP 1 Score: 366.3 bits (939), Expect = 8.8e-101
Identity = 435/1482 (29.35%), Postives = 646/1482 (43.59%), Query Frame = 0

Query: 14   EMKIPLFTVLKNGAILKNIFIVN--------------NVADRENEEVIILGRHPDCNIML 73
            E  IP+FTVLKNGAILKNIF+VN              +  D E EE++++GRHPDC+I+L
Sbjct: 17   EKTIPVFTVLKNGAILKNIFVVNSRDFSSPERNGSTVSDDDGEVEEILVVGRHPDCDILL 76

Query: 74   THPSISRFHLQIHSNPSSQKLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSR 133
            THPSISRFHL+I S  S QKL V DLSSVHGTWV   RIE    VE+ EGDT+R+GGS+R
Sbjct: 77   THPSISRFHLEIRSISSRQKLFVTDLSSVHGTWVRDLRIEPHGCVEVEEGDTIRIGGSTR 136

Query: 134  VYKLHWVPLSCAYDFQCP---------KEKKEEKVAIIEEEAVEDCEK------------ 193
            +Y+LHW+PLS AYD   P           ++EE+  ++E E +E  +             
Sbjct: 137  IYRLHWIPLSRAYDLDNPFVSPLDASTVLEQEEENRMLEAENLEVAQHQSLENTTSGDEG 196

Query: 194  ----DI------------------------------SLLDENKETVEDSVLENIEPLYSD 253
                D+                              S+L   +++VE   L+  E L + 
Sbjct: 197  VLHLDVTSEGTGSSVPSEDEDTYVTTREMSMPVASPSVLTLVRDSVETQKLQFNEDLQTS 256

Query: 254  ENWNMEMKEVPLAAPL------------GEVEGMVMSSV-------------DGGESVND 313
              W++++ E  +A  L            G+VEG+  S +             DGG  +N 
Sbjct: 257  PKWDLDVIE-SVAEKLSGSFVRSTQQSGGDVEGLGCSELFDAAEADECDVRGDGGLHLNV 316

Query: 314  LRNECKK--------------EEISLLSKPFGDE------------------------LK 373
            +  + +               +  SL +   GD+                        + 
Sbjct: 317  ISEKMESSVPNMIEAENLEVAQHQSLANTALGDDEDLHLDVTSEGTGSSVPSEDEDTYIT 376

Query: 374  SLEMSPQPPFLPLSAENL------SFNVESIIMSSFF-------------GSESKSSS-- 433
            ++E+S     +PL++ N+      S   + +  +  F              +E  SSS  
Sbjct: 377  TMEIS-----VPLASPNVLTLARDSIKTQKLQSTQDFQTPTMWDLDVVEAAAEKPSSSCV 436

Query: 434  --------------------SSTFEQNDSSSIWNIPLENVS---RSSLSEKQQCQSKIES 493
                                ++  ++ D     ++ L  +S    SS+S K+     + +
Sbjct: 437  LGKKLSGGYVEELGCFELFVAAEADKCDVRGDGSLHLNEISERMESSMSNKED-DPFLAA 496

Query: 494  PQPPSLPLSAENLS----FNVENIIMSSFF-------DAETKSSSANVFERE-----ETS 553
             +  SLPLS + ++    + VE++  S  F       +AE  SS  +    +     ETS
Sbjct: 497  KETSSLPLSTDFINPETLWLVEDVQASPEFCTSSVKANAENPSSGCSPSTEQIDGCFETS 556

Query: 554  SIWTIRLENKSNILSSVDDITATSENGYQQLDNENKSPQPRSSLLCKESSSDVEDTAMIA 613
                  L  +   LS   +++  +E   +++   +  P  ++ +   E + + ED+  + 
Sbjct: 557  GCSAFDLAAEVESLSLHQEVSEETEFVTKEVMGVSSEPLGKADIRSHEENGESEDSRQVI 616

Query: 614  FA---------------GGECDSS------SINEFEQKE-----INNVMTIPLGHELSNV 673
                              GE + S      S   F + E     +       +G E  + 
Sbjct: 617  EVSAEPVAKADIQSHEENGETEGSRQVIEVSPKSFSEAEPTIEILTGEAQGIIGSEFPSE 676

Query: 674  ALVDEEKLILDHERQQFDNENGQLLAEKNFSSNAEVVEEIQNLPPVHEVKSSSTQPPLAL 733
              V+ E   L H++   + +N ++ + +++    +  E   + P +    SS + P   L
Sbjct: 677  LAVETESENLLHQKSIGETKN-EIRSHEDYGETEDYGETECSWPDIAVSPSSVSPPEPTL 736

Query: 734  QSKTVTEK--------KEFMVEHELHPLEDDHSSVTTEKGI------QEMEILNK----- 793
            +  T   +         E  VE E+  L    S+V T+  I       E E+  +     
Sbjct: 737  EILTDEARGLLGSEFLSEVTVETEIENLLHQKSNVETKADILIHEDYGETEVSRQIITVS 796

Query: 794  ----SQAE---------------VGEDCVDKGNEELYQVTEC------------------ 853
                S+AE               VG D   +   E+   TEC                  
Sbjct: 797  PNSFSKAEPTLETEDSRQQARGLVGSD--SEFQSEVAMKTECENLLNQKRNGETKVSSRQ 856

Query: 854  ---LLDCQSQYK---------------NDSSMKTTSEVTPNSTMNQNVEYCIE-EKYSTS 913
               + DC S  K               + S   + SEV P +  +Q      E EK  T 
Sbjct: 857  ASPVSDCLSTPKDRLSSINTDDIQSLCSSSQPPSESEVNPATDQDQESGIISETEKPKTE 916

Query: 914  L----ETIEPSKSVPEKDYVHKEQLSEPSFVPCATEFVYSSLPD----EEVSPKIDVQIE 973
            L       E   S+ E +      +   S  P  +     +  D    EE+S       E
Sbjct: 917  LLIGSGRSEKYYSLSEIEGEENTDIGRLSRCPIPSALAAKTSEDTKLIEELSSSDSGSQE 976

Query: 974  NQTPDENVDVTTPIRSENISAVGGSIWSRRGKPTSFPRIETDVD--RANRVGTTLMDEIN 1033
            NQTP+ +  V   +  +  S+   +IWSRRGK  S  +I T+    +  + G    D+++
Sbjct: 977  NQTPETHA-VRDDVLCDMDSSSTCNIWSRRGKAASVLKIRTNKSQGKQKQTGRQPKDKLH 1036

Query: 1034 HEITGDERVANTLLSHLDGEEEEEIFTPDKENFTPNTLLMKSLKKKANIEDS-GNFSRSS 1093
             +    ++  +  + H     E EIFTPDKEN TP++ ++K L+   +++DS  +   S 
Sbjct: 1037 RKQALSDKSISLTIHHGAEILEPEIFTPDKENLTPSSHMLKRLQDIGDVKDSKSSLKLSG 1096

Query: 1094 KSQTSIFKSRHKVKQEEELSEES----DKENQTP-------------------------- 1137
            KS +S+  S   V   E  +E      DKEN TP                          
Sbjct: 1097 KSCSSLVHSSIAVLASEAFTEPEIFTPDKENLTPSSHMLKRLREFGDIKDTKGSSSKATR 1156

BLAST of Lcy12g006250 vs. TAIR 10
Match: AT5G38840.1 (SMAD/FHA domain-containing protein )

HSP 1 Score: 64.7 bits (156), Expect = 5.5e-10
Identity = 37/100 (37.00%), Postives = 53/100 (53.00%), Query Frame = 0

Query: 22  VLKNGAILKNIFIVNNVADRENEEVIILGRHPDCNIMLTHPSISRFHLQIHSNPSSQKLC 81
           VLK GAI++ +       D   +   + GR   C+  L HPSISRFH  I    S     
Sbjct: 107 VLKEGAIVEKL-------DVYKKGAYLFGRDGICDFALEHPSISRFHAVIQYKRSGAAY- 166

Query: 82  VVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVY 122
           + DL S HGT V+  +++    V++  GD +R GGS+R+Y
Sbjct: 167 IFDLGSTHGTTVNKNKVDKKVFVDLNVGDVIRFGGSTRLY 198

BLAST of Lcy12g006250 vs. TAIR 10
Match: AT5G47790.1 (SMAD/FHA domain-containing protein )

HSP 1 Score: 62.4 bits (150), Expect = 2.7e-09
Identity = 40/105 (38.10%), Postives = 57/105 (54.29%), Query Frame = 0

Query: 22  VLKNGAILKNIFIVNNVADRENEEVIILGR-HPDCNIMLTHPSISRFHLQI--HSNPSSQ 81
           V+K+G IL  I +     DR      I GR H  C+ +L H S+SR H  +  H N S  
Sbjct: 70  VVKDGQILDRIHL-----DRRRH---IFGRQHQTCDFVLDHQSVSRQHAAVVPHKNGS-- 129

Query: 82  KLCVVDLSSVHGTWVSGKRIEAGNRVEMREGDTLRVGGSSRVYKL 124
            + V+DL S HGT+V+ +R+     VE+  G +LR   S+R+Y L
Sbjct: 130 -IFVIDLGSAHGTFVANERLTKDTPVELEVGQSLRFAASTRIYLL 163