Lcy12g005490 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g005490
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	ATP-dependent DNA helicase
Location	Chr12: 21875199 .. 21876263 (-)
RNA-Seq Expression	Lcy12g005490
Synteny	Lcy12g005490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTACTATAAGATCGAAAAGGATGATAGCAATTGCAACAGCAACATCAGGTGTTGCTGCTTCGATTCTGCCAGTAGGACGGACTGCTCATTTTAGATTTAACACACCACTTCAAGCAAATGAATCATCAATATGCAATATTTCTAAGCAAAGTCATAAAGTAGAATTAATTAGACAAGCAAAAGTCATTATTTGGGATAAGACACCAATGGCCAAAAAAGCCACAATAGAGGTTGTTGACAAGACTTTTCGAGATATAATGGATAGTTCAGAGCCATTTGGTGGAAAAGTTGTCGTCTTTGAAGGAGATTTTCGACAAGTACTACCTGTTGTCCCACGAGCAACAAGACAACAGACTATCAATGAAAGTTTGGTAAAGTCATACCTTTGGGAACAAATGGAAAAACTACAACTAACCAAAAATATGAGAGCCCAACATGACAGAAATTTTTGCAGAATATCTATTAAGGATTGATAATGGAACAAAACCATTTGTAGAAGAAGACTATATTTGCCTCCCAAATGATGTTGTCATTACGAGTGAAAGCGAAAATGATGCAGTATTAAAATTATTGAATATTGTTTATCCTGATCTTGAAAAAAATGCTACTTTGATAGAGTATATTACAAGTAGGGCAATTTTGTCAACCACAAATGAATATGTTGATCAAATGTATGAGAAAATGATTCATCTATTTCCTGGAAAAATAGAAGAATTCATGAGTTTTGATGAGGCGATTGACAACACACATAATTACTACCAAGAAGAATTCCTCAATTCATTATTGCCCAATGGTGTCCCTCCTCACAAATTGATATTGAAAAAACATTGTCCAGTCATGCTATTGAGAAACTTAGACCCACCAAACGGTCTATGTAATGGAACTCGAATGGTTTGTCATGAATTTCACAAAAATGTCATTTATACAGAAATTGCAGCTGGACAACATGCAAGAAAGAAAGTTTTTCTTCCACGCATACCAATGAGCCCTACAAATGATGAAGGATATCCATTTAAATTTAAAAGAAAACAATTTCCCGTACGACTCTGCTTTGCCATGACTTAA

mRNA sequence

CTACTATAAGATCGAAAAGGATGATAGCAATTGCAACAGCAACATCAGGTGTTGCTGCTTCGATTCTGCCAGTAGGACGGACTGCTCATTTTAGATTTAACACACCACTTCAAGCAAATGAATCATCAATATGCAATATTTCTAAGCAAAGTCATAAAGTAGAATTAATTAGACAAGCAAAAGTCATTATTTGGGATAAGACACCAATGGCCAAAAAAGCCACAATAGAGGTTGTTGACAAGACTTTTCGAGATATAATGGATAGTTCAGAGCCATTTGGTGGAAAAGTTGTCGTCTTTGAAGGAGATTTTCGACAAGTACTACCTGTTGTCCCACGAGCAACAAGACAACAGACTATCAATGAAAGTTTGGTAAAGATTGATAATGGAACAAAACCATTTGTAGAAGAAGACTATATTTGCCTCCCAAATGATGTTGTCATTACGAGTGAAAGCGAAAATGATGCAGTATTAAAATTATTGAATATTGTTTATCCTGATCTTGAAAAAAATGCTACTTTGATAGAGTATATTACAAGTAGGGCAATTTTGTCAACCACAAATGAATATGTTGATCAAATGTATGAGAAAATGATTCATCTATTTCCTGGAAAAATAGAAGAATTCATGAGTTTTGATGAGGCGATTGACAACACACATAATTACTACCAAGAAGAATTCCTCAATTCATTATTGCCCAATGGTGTCCCTCCTCACAAATTGATATTGAAAAAACATTGTCCAGTCATGCTATTGAGAAACTTAGACCCACCAAACGGTCTATGTAATGGAACTCGAATGGTTTGTCATGAATTTCACAAAAATGTCATTTATACAGAAATTGCAGCTGGACAACATGCAAGAAAGAAAGTTTTTCTTCCACGCATACCAATGAGCCCTACAAATGATGAAGGATATCCATTTAAATTTAAAAGAAAACAATTTCCCGTACGACTCTGCTTTGCCATGACTTAA

Coding sequence (CDS)

CTACTATAAGATCGAAAAGGATGATAGCAATTGCAACAGCAACATCAGGTGTTGCTGCTTCGATTCTGCCAGTAGGACGGACTGCTCATTTTAGATTTAACACACCACTTCAAGCAAATGAATCATCAATATGCAATATTTCTAAGCAAAGTCATAAAGTAGAATTAATTAGACAAGCAAAAGTCATTATTTGGGATAAGACACCAATGGCCAAAAAAGCCACAATAGAGGTTGTTGACAAGACTTTTCGAGATATAATGGATAGTTCAGAGCCATTTGGTGGAAAAGTTGTCGTCTTTGAAGGAGATTTTCGACAAGTACTACCTGTTGTCCCACGAGCAACAAGACAACAGACTATCAATGAAAGTTTGGTAAAGATTGATAATGGAACAAAACCATTTGTAGAAGAAGACTATATTTGCCTCCCAAATGATGTTGTCATTACGAGTGAAAGCGAAAATGATGCAGTATTAAAATTATTGAATATTGTTTATCCTGATCTTGAAAAAAATGCTACTTTGATAGAGTATATTACAAGTAGGGCAATTTTGTCAACCACAAATGAATATGTTGATCAAATGTATGAGAAAATGATTCATCTATTTCCTGGAAAAATAGAAGAATTCATGAGTTTTGATGAGGCGATTGACAACACACATAATTACTACCAAGAAGAATTCCTCAATTCATTATTGCCCAATGGTGTCCCTCCTCACAAATTGATATTGAAAAAACATTGTCCAGTCATGCTATTGAGAAACTTAGACCCACCAAACGGTCTATGTAATGGAACTCGAATGGTTTGTCATGAATTTCACAAAAATGTCATTTATACAGAAATTGCAGCTGGACAACATGCAAGAAAGAAAGTTTTTCTTCCACGCATACCAATGAGCCCTACAAATGATGAAGGATATCCATTTAAATTTAAAAGAAAACAATTTCCCGTACGACTCTGCTTTGCCATGACTTAA

Protein sequence

TIRSKRMIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKVIIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINESLVKIDNGTKPFVEEDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVIYTEIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT

Homology

[Close]

BLAST of Lcy12g005490 vs. ExPASy Swiss-Prot
Match: Q9UUA2 (ATP-dependent DNA helicase pfh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pfh1 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 8.6e-06
Identity = 72/268 (26.87%), Postives = 118/268 (44.03%), Query Frame = 0


Query: 12  TATSGVAASILPVGRTAHFRFNTPL--QANESSICNISKQSHKVELIRQAKVIIWDKTPM 71
           TA++G+AA  +  G T H      L  ++ +  +  I K    V    + +V+I D+  M
Sbjct: 363 TASTGLAACNIG-GVTLHSFAGVGLARESVDLLVSKIKKNKKCVNRWLRTRVLIIDEVSM 422

Query: 72  AKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINESLVKIDNG 131
                ++ +++  R I   S+PFGG  +V  GDF Q LP VP     +   ES    ++ 
Sbjct: 423 VDAELMDKLEEVARVIRKDSKPFGGIQLVLTGDFFQ-LPPVP-----ENGKESKFCFESQ 482

Query: 132 TKPFVEEDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTTNEY 191
           T     +  I L +        +++  +K+LN    +L       E +    +L+ T EY
Sbjct: 483 TWKSALDFTIGLTH----VFRQKDEEFVKMLN----ELRLGKLSDESVRKFKVLNRTIEY 542

Query: 192 VDQMYEKMIHLFPGKIEEFMSFD-------------EAIDNTHNYYQEEFLNSLLPNGVP 251
            D +      LFP + E   S D              AID +     +EF + LL   + 
Sbjct: 543 EDGLLP--TELFPTRYEVERSNDMRMQQINQNPVTFTAID-SGTVRDKEFRDRLLQGCMA 602

Query: 252 PHKLILKKHCPVMLLRNLDPPNGLCNGT 265
           P  L+LK +  VML++N+D  + L NG+
Sbjct: 603 PATLVLKVNAQVMLIKNID--DQLVNGS 610

[Close]

BLAST of Lcy12g005490 vs. ExPASy Swiss-Prot
Match: P38766 (ATP-dependent DNA helicase RRM3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RRM3 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.9e-05
Identity = 68/283 (24.03%), Postives = 122/283 (43.11%), Query Frame = 0


Query: 1   TIRSKRMIAIATATSGVAASILPVGRTAHFRFNTPL--QANESSICNISKQSHKVELIRQ 60
           ++  K  IAI TA++G+AA  +  G T H      +  +  +  +  I  Q   +   R 
Sbjct: 273 SLYGKESIAI-TASTGLAAVTIG-GSTLHKWSGIGIGNKTIDQLVKKIQSQKDLLAAWRY 332

Query: 61  AKVIIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQT 120
            KV+I D+  M     ++ +++  R I  + +PFGG  +V  GDF Q+ PV         
Sbjct: 333 TKVLIIDEISMVDGNLLDKLEQIARRIRKNDDPFGGIQLVLTGDFFQLPPVAK------- 392

Query: 121 INESLVKIDNGTKPFVEEDYI---CLPNDVVITS--ESENDAVLKLLNIV---------- 180
                 K ++    F  E  +   C+   +++T     +++ ++ +LN +          
Sbjct: 393 ------KDEHNVVKFCFESEMWKRCIQKTILLTKVFRQQDNKLIDILNAIRYGELTVDIA 452

Query: 181 --YPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNTHNY 240
               +L ++    + I    + +T  E V+    K +   PG + EF    +A+DN    
Sbjct: 453 KTIRNLNRDIDYADGIAPTELYATRRE-VELSNVKKLQSLPGDLYEF----KAVDNAPER 512

Query: 241 YQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGT 265
           YQ    +SL+   V    + LK+   VM+L+N  P   L NG+
Sbjct: 513 YQAILDSSLMVEKV----VALKEDAQVMMLKN-KPDVELVNGS 530

[Close]

BLAST of Lcy12g005490 vs. ExPASy Swiss-Prot
Match: A5WFR0 (ATP-dependent DNA helicase pif1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 GN=pif1 PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.5e-05
Identity = 69/280 (24.64%), Postives = 122/280 (43.57%), Query Frame = 0


Query: 2   IRSKRMIAIATATSGVAASILPVGRTAHFRFNTPL--QANESSICNISKQSHKVELIRQA 61
           +R++R+    TA++G+AA+ +  G T H      +  +  E  + N+S++    + +++ 
Sbjct: 37  LRARRVPVATTASTGIAATHMN-GITIHSWSGIGIKDELTERDLVNLSRKKVLKDRLQET 96

Query: 62  KVIIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPR--ATRQQ 121
            V+I D+  M     + +V++  + +  + +PFGG  VV  GDF Q+ PV  R  + R +
Sbjct: 97  AVLIIDEISMLHAKQLNLVNQVLKHMRQNDKPFGGIQVVVAGDFFQLPPVGSRGESNRDK 156

Query: 122 TINESLVKIDNGTKPFVEEDYICLPNDVVITSESE-NDAVLKLLNIVYPDLEKNATLIEY 181
               S   +D G K       IC  ++       E  DA + L NI+     +N      
Sbjct: 157 FAFMSQAWLDAGFK-------ICYLSEQHRQQSGEGEDAQITLDNILNQIRGENGVSAAA 216

Query: 182 I------------TSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNTHNYYQE 241
           I             +R  L T N  V+++ E  + L  G+   +     AI +  N   E
Sbjct: 217 IAALQNTFYQDVDVNRTRLYTHNVNVNKINEHELALLEGETVTY----NAIAHGDNKLVE 276

Query: 242 EFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGT 265
               S+  +     +L LK    VM ++N +   G+ NGT
Sbjct: 277 TLKKSVRTS----DELTLKTGAKVMFIKN-NSELGVSNGT 299

[Close]

BLAST of Lcy12g005490 vs. ExPASy Swiss-Prot
Match: Q0R4F1 (ATP-dependent DNA helicase PIF1 OS=Xenopus laevis OX=8355 GN=pif1 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.3e-05
Identity = 67/272 (24.63%), Postives = 118/272 (43.38%), Query Frame = 0


Query: 11  ATATSGVAASILPVGRTAH-FRFNTPLQANESSICNISKQSHKVELIRQAKVIIWDKTPM 70
           ATA++GVAA  +  G T H F      +A+      ++K+    +     K +I D+  M
Sbjct: 253 ATASTGVAACHIG-GTTLHAFAGIGSGKASLEQCIELAKRPGVRQHWTSCKHLIIDEISM 312

Query: 71  AKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTI--NESLVKID 130
            +    + ++   R +    EPFGG  ++  GDF Q+ PV   +++ +     +S  K  
Sbjct: 313 VEGEFFDKLEAVARAVRGKDEPFGGIQLIVCGDFLQLPPVTQASSQTKFCFQGKSWRKCI 372

Query: 131 NGTKPFVE------EDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNATLIEYITSRA 190
           + T    E      +++I L   + +     +D   +LL      +E++  L        
Sbjct: 373 HLTMELTEVRRQTDKNFISLLQAIRL-GRCTDDVARQLLQTTNHKVERDGIL------AT 432

Query: 191 ILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNTHNYYQEEFLNSLLPNGVPPHKLI 250
            L T  + V+   E+ +   PG+   +    EA+D+     +   +N+  P      ++ 
Sbjct: 433 RLCTHKDDVEITNERRLQQLPGESHSY----EALDSDPMLVKT--INAQCPVN---QQIQ 492

Query: 251 LKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHK 274
           LKK   VML +NLD   GL NG R V  +F +
Sbjct: 493 LKKGAQVMLAKNLDVSRGLVNGARGVVIKFEE 507

[Close]

BLAST of Lcy12g005490 vs. ExPASy Swiss-Prot
Match: Q7ZV90 (ATP-dependent DNA helicase PIF1 OS=Danio rerio OX=7955 GN=pif1 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 4.7e-04
Identity = 68/275 (24.73%), Postives = 114/275 (41.45%), Query Frame = 0


Query: 11  ATATSGVAASILPVGRTAH-FRFNTPLQANESSICNISKQSHKVELIRQAKVIIWDKTPM 70
           ATA++GVAA  +  G T H F       A       ++++   +      K +I D+  M
Sbjct: 252 ATASTGVAACHIG-GTTLHSFAGIGSGSAPLEQCIELAQRPGVLRHWTSCKHLIIDEISM 311

Query: 71  AKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQ------------ 130
            +    + ++   R I  S+EPFGG  ++  GDF Q+ PV     +              
Sbjct: 312 VEAEFFDKLEAIARSIRRSTEPFGGIQLIVCGDFLQLPPVTKGKEKANFCFQSRSWRKCI 371

Query: 131 TINESLVKIDNGTKPFVEEDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNATLIEYI 190
            +N  L+++   T    ++ +I L   V +   +E +   +LL      +E++  L    
Sbjct: 372 HMNMELMEVRRQT----DKTFISLLQAVRVGRVTE-EVTAQLLKSANHCIERDGIL---- 431

Query: 191 TSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNTHNYYQEEFLNSLLPNGVPP 250
                L T  + V+   E  +   PG +  +    EA+D+      +  L   +    P 
Sbjct: 432 --ATRLCTHKDDVELTNENKLKQLPGVVRMY----EAVDS------DPMLVQTIDAQSPV 491

Query: 251 HKLI-LKKHCPVMLLRNLDPPNGLCNGTRMVCHEF 272
            +L+ LK    VML +NLD   GL NG R V  +F
Sbjct: 492 SRLLQLKVGAQVMLTKNLDVQRGLVNGARGVVVDF 504

[Close]

BLAST of Lcy12g005490 vs. ExPASy TrEMBL
Match: A0A6J1DMF2 (ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111022374 PE=3 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 2.0e-122
Identity = 225/327 (68.81%), Postives = 258/327 (78.90%), Query Frame = 0


Query: 7   MIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKVIIWDK 66
           MIAIATATSGVAA I+P GRTAH RFN PLQANESS+CNISKQS++ EL+RQAK+IIWD+
Sbjct: 1   MIAIATATSGVAAXIMPGGRTAHSRFNIPLQANESSMCNISKQSNRAELVRQAKLIIWDE 60

Query: 67  TPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINESLV-- 126
            P AKK TIE VD+TF+DIM++S+PFGGKV+VF GDFRQVLPVVPRATRQQT++ESLV  
Sbjct: 61  APXAKKVTIEAVDRTFQDIMENSKPFGGKVIVFGGDFRQVLPVVPRATRQQTVHESLVKS 120

Query: 127 ----------------------------KIDNGTKPFVEEDYICLPNDVVITSESENDAV 186
                                       KI NGT+P + EDY+ LP D+ I  E   D+V
Sbjct: 121 YLWKQMEKLQLKKNMRAQNDYQFAEYLLKIGNGTEPSIVEDYVRLPKDIAIIDEGHEDSV 180

Query: 187 LKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAID 246
           LKLLNIVYPDL +NA   EYIT+RAILSTTNEYVD++  KMI LF G IEEFMSFDE ID
Sbjct: 181 LKLLNIVYPDLPQNAMSAEYITNRAILSTTNEYVDEINNKMIRLFLGNIEEFMSFDETID 240

Query: 247 NTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVI 304
           +TH+YYQEEFLNSLLPNG+PPHKLILK +CP++LLRNLDP NGLCNGTRMVC +F KNVI
Sbjct: 241 DTHSYYQEEFLNSLLPNGIPPHKLILKINCPIILLRNLDPSNGLCNGTRMVCRKFQKNVI 300

[Close]

BLAST of Lcy12g005490 vs. ExPASy TrEMBL
Match: A0A5B7A9C3 (ATP-dependent DNA helicase OS=Davidia involucrata OX=16924 GN=Din_021512 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 3.6e-116
Identity = 208/352 (59.09%), Postives = 262/352 (74.43%), Query Frame = 0


Query: 2    IRSKRMIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKV 61
            +RS++MIA+ATATSGVAA+I+P GRTAH RF  P+  NE+SICNI KQS   EL+R+A++
Sbjct: 1074 LRSRKMIALATATSGVAAAIMPGGRTAHSRFKIPISVNETSICNIPKQSGTAELLRKARL 1133

Query: 62   IIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINE 121
            IIWD+TPMAK+  IE +D+T RDIM++ E FGGKV+VF GDFRQVLPVVPRATR++T+N 
Sbjct: 1134 IIWDETPMAKRWAIETLDRTLRDIMNNQESFGGKVIVFGGDFRQVLPVVPRATREETVNA 1193

Query: 122  SLVK------------------------------IDNGTKPFVEEDYICLPNDVVITSES 181
            SLVK                              + NG +P   +D I +P+ +VI  E+
Sbjct: 1194 SLVKSHIWSKMEKLTLTRNMRARADPNFSDFLLRVGNGEEPTNNQDMILIPDQMVIKYEN 1253

Query: 182  ENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSF 241
            +  +  KL++ +YP LE+NA   EY+T RAIL+T NEYVD++ EK+I +FPG +  F SF
Sbjct: 1254 DEVSEQKLIDDIYPSLEQNAKSAEYMTKRAILATKNEYVDKLNEKLIEMFPGDVRTFNSF 1313

Query: 242  DEAIDNTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEF 301
            DEAID+THNYYQEEFLNSL PNG+PPH+L+LK + P+MLLRNLDP NGLCNGTRMVC  F
Sbjct: 1314 DEAIDDTHNYYQEEFLNSLTPNGLPPHRLVLKVNSPIMLLRNLDPSNGLCNGTRMVCRNF 1373

Query: 302  HKNVIYTEIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
              NVI+ EI  GQHA K+V LPRIP+SP  +EGYPF+FKRKQFP+RLCFAMT
Sbjct: 1374 ESNVIHAEITVGQHAGKQVLLPRIPLSPAENEGYPFQFKRKQFPIRLCFAMT 1425

[Close]

BLAST of Lcy12g005490 vs. ExPASy TrEMBL
Match: A0A5B7A5M4 (ATP-dependent DNA helicase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_021511 PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.4e-115
Identity = 207/352 (58.81%), Postives = 262/352 (74.43%), Query Frame = 0


Query: 2   IRSKRMIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKV 61
           +RS++MIA+ATATSGVAA+I+P GRTAH RF  P+  NE+SICNI KQS   EL+R+A++
Sbjct: 48  LRSRKMIALATATSGVAAAIMPGGRTAHSRFKIPISVNETSICNIPKQSGTAELLRKARL 107

Query: 62  IIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINE 121
           IIWD+TPMAK+  IE +D+T +DIM++ E FGGKV+VF GDFRQVLPVVPRATR++T+N 
Sbjct: 108 IIWDETPMAKRWAIETLDRTLKDIMNNQESFGGKVIVFGGDFRQVLPVVPRATREETVNA 167

Query: 122 SLVK------------------------------IDNGTKPFVEEDYICLPNDVVITSES 181
           SLVK                              + NG +P   +D I +P+ +VI  E+
Sbjct: 168 SLVKSHIWSKMEKLTLTRNMRARADPNFSDFLLRVGNGEEPTNNQDMILIPDQMVIKYEN 227

Query: 182 ENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSF 241
           +  +  KL++ +YP LE+NA   EY+T RAIL+T NEYVD++ EK+I +FPG +  F SF
Sbjct: 228 DEVSEQKLIDDIYPFLEQNAKSAEYMTKRAILATKNEYVDKLNEKLIEMFPGDVRTFNSF 287

Query: 242 DEAIDNTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEF 301
           DEAID+THNYYQEEFLNSL PNG+PPH+L+LK + P+MLLRNLDP NGLCNGTRMVC  F
Sbjct: 288 DEAIDDTHNYYQEEFLNSLTPNGLPPHRLVLKVNSPIMLLRNLDPSNGLCNGTRMVCRNF 347

Query: 302 HKNVIYTEIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
             NVI+ EI  GQHA K+V LPRIP+SP  +EGYPF+FKRKQFP+RLCFAMT
Sbjct: 348 ESNVIHAEITIGQHAGKQVLLPRIPLSPAENEGYPFQFKRKQFPIRLCFAMT 399

[Close]

BLAST of Lcy12g005490 vs. ExPASy TrEMBL
Match: A0A6J1DFU4 (ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111020055 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.9e-113
Identity = 204/285 (71.58%), Postives = 229/285 (80.35%), Query Frame = 0


Query: 69  MAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINESLV---- 128
           MAKK TIE VD+TFRDIMDS+EPFGGK+VVF GDFRQVLPVVPRATRQQTINESLV    
Sbjct: 1   MAKKTTIEAVDRTFRDIMDSTEPFGGKIVVFGGDFRQVLPVVPRATRQQTINESLVKSYL 60

Query: 129 --------------------------KIDNGTKPFVEEDYICLPNDVVITSESENDAVLK 188
                                     KI NGT+P VE+DYICL +D+ IT E++  A++K
Sbjct: 61  WKQMEKLQLTKNMRAHSDSQFAEYLLKIGNGTEPSVEDDYICLTDDITITGENDEGAIMK 120

Query: 189 LLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNT 248
           LLN+VYPDLE+NAT  EY+T+R ILSTTNEYVDQ+ EKMI LFPGK  EFMSFDEAID+T
Sbjct: 121 LLNVVYPDLEQNATSSEYMTNRVILSTTNEYVDQINEKMIQLFPGKFFEFMSFDEAIDDT 180

Query: 249 HNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVIYT 308
           HNYYQEEFLNSLLPNG+PPHKL+LK +CPV+LLRNLDP NGLCNGTRMVC EF KN+IY 
Sbjct: 181 HNYYQEEFLNSLLPNGIPPHKLMLKANCPVILLRNLDPANGLCNGTRMVCREFRKNIIYA 240

Query: 309 EIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
           EI  GQ+A K+VFLPRIPMSP NDEGYPFKFKRKQFP+RLCFAMT
Sbjct: 241 EITTGQNAGKQVFLPRIPMSPANDEGYPFKFKRKQFPIRLCFAMT 285

[Close]

BLAST of Lcy12g005490 vs. ExPASy TrEMBL
Match: A0A1U7WW73 (ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104231538 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 4.3e-101
Identity = 186/317 (58.68%), Postives = 236/317 (74.45%), Query Frame = 0


Query: 7   MIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKVIIWDK 66
           MI +ATATSG+A +ILP GRTAH RF+ PLQ +E++I N+SKQS   +LIR+AK+IIWD+
Sbjct: 1   MIVLATATSGIATTILPGGRTAHSRFDIPLQTSETTITNMSKQSGGAKLIRKAKLIIWDE 60

Query: 67  TPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINESLVKI 126
            PMA++ TIE +D++FRDIMD  EPFGGKV+VF GDFRQVLPVVP+ATR +T+N SL  I
Sbjct: 61  APMARRQTIETIDRSFRDIMDIDEPFGGKVMVFGGDFRQVLPVVPKATRAETVNASLHTI 120

Query: 127 DNGTKPFVEEDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTT 186
                   ++D I LP  +VI          +L+  ++P L +N +  +Y+T RAIL++ 
Sbjct: 121 --------KDDMILLPEQLVIKPNGNISGEDRLITKIFPSLNENGSCAKYMTERAILASR 180

Query: 187 NEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNTHNYYQEEFLNSLLPNGVPPHKLILKKHC 246
           NEYVDQ+ E +I  FPG+ + F SFD A D+T+NYYQEE+LN+L PNG+PPH+LILKK+ 
Sbjct: 181 NEYVDQLNEMLIDKFPGESKIFHSFDSAEDDTNNYYQEEYLNTLTPNGLPPHRLILKKNA 240

Query: 247 PVMLLRNLDPPNGLCNGTRMVCHEFHKNVIYTEIAAGQHARKKVFLPRIPMSPTNDEGYP 306
           P+MLLRNLDP N LCN TRMVC  F  N I+ EI  GQ   K VF+PRI +SP  +EGYP
Sbjct: 241 PIMLLRNLDPSNSLCNSTRMVCRGFDNNAIHAEIMMGQCTSKHVFIPRIQLSPPENEGYP 300

Query: 307 FKFKRKQFPVRLCFAMT 324
           FKF RKQFPVRLCFAMT
Sbjct: 301 FKFVRKQFPVRLCFAMT 309

[Close]

BLAST of Lcy12g005490 vs. NCBI nr
Match: XP_022155233.1 (ATP-dependent DNA helicase PIF1-like [Momordica charantia])

HSP 1 Score: 448.7 bits (1153), Expect = 4.1e-122
Identity = 225/327 (68.81%), Postives = 258/327 (78.90%), Query Frame = 0


Query: 7   MIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKVIIWDK 66
           MIAIATATSGVAA I+P GRTAH RFN PLQANESS+CNISKQS++ EL+RQAK+IIWD+
Sbjct: 1   MIAIATATSGVAAXIMPGGRTAHSRFNIPLQANESSMCNISKQSNRAELVRQAKLIIWDE 60

Query: 67  TPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINESLV-- 126
            P AKK TIE VD+TF+DIM++S+PFGGKV+VF GDFRQVLPVVPRATRQQT++ESLV  
Sbjct: 61  APXAKKVTIEAVDRTFQDIMENSKPFGGKVIVFGGDFRQVLPVVPRATRQQTVHESLVKS 120

Query: 127 ----------------------------KIDNGTKPFVEEDYICLPNDVVITSESENDAV 186
                                       KI NGT+P + EDY+ LP D+ I  E   D+V
Sbjct: 121 YLWKQMEKLQLKKNMRAQNDYQFAEYLLKIGNGTEPSIVEDYVRLPKDIAIIDEGHEDSV 180

Query: 187 LKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAID 246
           LKLLNIVYPDL +NA   EYIT+RAILSTTNEYVD++  KMI LF G IEEFMSFDE ID
Sbjct: 181 LKLLNIVYPDLPQNAMSAEYITNRAILSTTNEYVDEINNKMIRLFLGNIEEFMSFDETID 240

Query: 247 NTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVI 304
           +TH+YYQEEFLNSLLPNG+PPHKLILK +CP++LLRNLDP NGLCNGTRMVC +F KNVI
Sbjct: 241 DTHSYYQEEFLNSLLPNGIPPHKLILKINCPIILLRNLDPSNGLCNGTRMVCRKFQKNVI 300

[Close]

BLAST of Lcy12g005490 vs. NCBI nr
Match: XP_022152307.1 (ATP-dependent DNA helicase PIF1-like [Momordica charantia])

HSP 1 Score: 418.3 bits (1074), Expect = 6.0e-113
Identity = 204/285 (71.58%), Postives = 229/285 (80.35%), Query Frame = 0


Query: 69  MAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINESLV---- 128
           MAKK TIE VD+TFRDIMDS+EPFGGK+VVF GDFRQVLPVVPRATRQQTINESLV    
Sbjct: 1   MAKKTTIEAVDRTFRDIMDSTEPFGGKIVVFGGDFRQVLPVVPRATRQQTINESLVKSYL 60

Query: 129 --------------------------KIDNGTKPFVEEDYICLPNDVVITSESENDAVLK 188
                                     KI NGT+P VE+DYICL +D+ IT E++  A++K
Sbjct: 61  WKQMEKLQLTKNMRAHSDSQFAEYLLKIGNGTEPSVEDDYICLTDDITITGENDEGAIMK 120

Query: 189 LLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFDEAIDNT 248
           LLN+VYPDLE+NAT  EY+T+R ILSTTNEYVDQ+ EKMI LFPGK  EFMSFDEAID+T
Sbjct: 121 LLNVVYPDLEQNATSSEYMTNRVILSTTNEYVDQINEKMIQLFPGKFFEFMSFDEAIDDT 180

Query: 249 HNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVIYT 308
           HNYYQEEFLNSLLPNG+PPHKL+LK +CPV+LLRNLDP NGLCNGTRMVC EF KN+IY 
Sbjct: 181 HNYYQEEFLNSLLPNGIPPHKLMLKANCPVILLRNLDPANGLCNGTRMVCREFRKNIIYA 240

Query: 309 EIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
           EI  GQ+A K+VFLPRIPMSP NDEGYPFKFKRKQFP+RLCFAMT
Sbjct: 241 EITTGQNAGKQVFLPRIPMSPANDEGYPFKFKRKQFPIRLCFAMT 285

[Close]

BLAST of Lcy12g005490 vs. NCBI nr
Match: XP_020242961.1 (uncharacterized protein LOC109821182 [Asparagus officinalis])

HSP 1 Score: 398.7 bits (1023), Expect = 4.9e-107
Identity = 192/352 (54.55%), Postives = 256/352 (72.73%), Query Frame = 0


Query: 2   IRSKRMIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKV 61
           +RS++MIA+ATATSGVAA I+P GRTAH RF  P+ ANE+++CNISKQ    +LIR A +
Sbjct: 576 LRSRKMIALATATSGVAAVIMPGGRTAHSRFKIPITANETTMCNISKQDGTSKLIRLASL 635

Query: 62  IIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINE 121
           IIWD+ PMAK+  IE VD+T RD+M     FGGKVVVF GDFRQVLPVVPR TR +T+N 
Sbjct: 636 IIWDEAPMAKRFAIETVDRTLRDVMGDKRIFGGKVVVFGGDFRQVLPVVPRGTRAETVNA 695

Query: 122 SLVK------------------------------IDNGTKPFVEEDYICLPNDVVITSES 181
           SLVK                              + NG +P ++E+ I LP+++V+  ++
Sbjct: 696 SLVKSYLWEKMEKLSLTINMRARTDQVFGEFLLRVGNGEEPTIDENLILLPHEMVVEYDN 755

Query: 182 ENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSF 241
           + ++   L++ ++P L+ N++  EY+T RAILST NEYVD + EK+I LFPG+ + F SF
Sbjct: 756 DENSEKNLIDAIFPSLQSNSSSAEYMTQRAILSTRNEYVDGLNEKLIALFPGEAKTFFSF 815

Query: 242 DEAIDNTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEF 301
            EA+D+T+NYYQEEFLN+L+PNG+PPHKLILK++C +MLLRNLDP NG+CNGTRM+   +
Sbjct: 816 VEAVDDTNNYYQEEFLNTLMPNGLPPHKLILKRNCLIMLLRNLDPSNGMCNGTRMIYRGY 875

Query: 302 HKNVIYTEIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
             NVI+ EI  GQH+ + V +PRIP+SP  +EGYPF+F+RKQFP+RLCFAMT
Sbjct: 876 EHNVIHAEITIGQHSGQNVMIPRIPLSPAENEGYPFQFRRKQFPIRLCFAMT 927

[Close]

BLAST of Lcy12g005490 vs. NCBI nr
Match: XP_012841137.1 (PREDICTED: uncharacterized protein LOC105961450 [Erythranthe guttata])

HSP 1 Score: 397.5 bits (1020), Expect = 1.1e-106
Identity = 199/352 (56.53%), Postives = 250/352 (71.02%), Query Frame = 0


Query: 2    IRSKRMIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAKV 61
            +RS++ IAIATATSGVAA+I+P GRTAH RF  P+ ANESS CNISKQS    L+R  K+
Sbjct: 906  LRSRKKIAIATATSGVAAAIMPGGRTAHSRFKIPIDANESSDCNISKQSGVANLLRTTKL 965

Query: 62   IIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTINE 121
            IIWD+ PMAK+  IE VDK F+DIM + E FGGKVVVF GDFRQVLPVVPR T  QTI+ 
Sbjct: 966  IIWDEAPMAKRWAIENVDKLFKDIMGNDEHFGGKVVVFGGDFRQVLPVVPRGTIHQTISA 1025

Query: 122  SLVK------------------------------IDNGTKPFVEEDYICLPNDVVITSES 181
            SLVK                              + NG +    E  I +P ++V+T ++
Sbjct: 1026 SLVKSRLWHKMIKFSLSKNMRAQKDPEFGDFLLRVGNGEETSDIEGNIIIPEEMVVTYDN 1085

Query: 182  ENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSF 241
            E D++ +L++ ++P L  NA    Y+T+RAIL++ NE VD++ +K+I +FPG    F SF
Sbjct: 1086 EEDSMARLIHSIFPSLSSNAESSTYMTTRAILASKNEEVDKLNDKLISMFPGDARTFQSF 1145

Query: 242  DEAIDNTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEF 301
            DEAID+T+N Y+E+FLNSL PNG+PPHKL+LK++CP++LLRNLDP NGLCNGTRMVC  F
Sbjct: 1146 DEAIDDTNNNYEEDFLNSLTPNGLPPHKLVLKRNCPIILLRNLDPSNGLCNGTRMVCRNF 1205

Query: 302  HKNVIYTEIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
              NVI  EI  GQH  K VF+PRIP+SP  +EGYPFKFKRKQFP+RLCFAMT
Sbjct: 1206 KSNVIDAEIVFGQHVGKHVFIPRIPLSPAENEGYPFKFKRKQFPIRLCFAMT 1257

[Close]

BLAST of Lcy12g005490 vs. NCBI nr
Match: XP_020275823.1 (ATP-dependent DNA helicase PIF1-like [Asparagus officinalis])

HSP 1 Score: 396.7 bits (1018), Expect = 1.9e-106
Identity = 196/353 (55.52%), Postives = 255/353 (72.24%), Query Frame = 0


Query: 1   TIRSKRMIAIATATSGVAASILPVGRTAHFRFNTPLQANESSICNISKQSHKVELIRQAK 60
           T+RS+ MIA+ATATSGVAA I+P GRTAH RF   +   +SS+C+ISKQ    +L+R A+
Sbjct: 276 TVRSQCMIALATATSGVAAVIMPGGRTAHSRFKIKIPTTDSSMCSISKQDGTSKLLRIAR 335

Query: 61  VIIWDKTPMAKKATIEVVDKTFRDIMDSSEPFGGKVVVFEGDFRQVLPVVPRATRQQTIN 120
           +IIWD+  MAK+  IE +D+T RD+MD  EPFGGKV+VF GDFRQVLPVVPR+TR QTI 
Sbjct: 336 LIIWDEAAMAKRVAIETLDRTLRDLMDKDEPFGGKVIVFGGDFRQVLPVVPRSTRSQTIK 395

Query: 121 ESLV------------------------------KIDNGTKPFVEEDYICLPNDVVITSE 180
           ESLV                              +I NG +P +  D+I LPN ++I ++
Sbjct: 396 ESLVSSHLWRKMIKLQLSKNMRAKSDTAFSEFLLRIGNGDEPTMTRDFIKLPNAMLIQNQ 455

Query: 181 SENDAVLKLLNIVYPDLEKNATLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMS 240
            +      L+N ++P L++N   I+Y+  RAIL+T NEYVD + +++I  FPG+ +EF S
Sbjct: 456 GDELPEDSLINSIFPSLDENFKSIDYMKDRAILATKNEYVDMLNDRLIKRFPGEGKEFYS 515

Query: 241 FDEAIDNTHNYYQEEFLNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHE 300
           FDEA+D+T+++YQEEFLN+LLPNG+PPHKL+LKK+CP++LLRNLDP N LCNGTRMVC +
Sbjct: 516 FDEAVDDTNHHYQEEFLNTLLPNGLPPHKLLLKKYCPIILLRNLDPTNRLCNGTRMVCQD 575

Query: 301 FHKNVIYTEIAAGQHARKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
           F+ NVI  +I   QHA K+VFLPRIP+SP  DEG PFKFKRKQFP+RLCFAMT
Sbjct: 576 FNYNVIQVKITMRQHAGKEVFLPRIPLSPAEDEGLPFKFKRKQFPIRLCFAMT 628

[Close]

BLAST of Lcy12g005490 vs. TAIR 10
Match: AT5G28780.1 (PIF1 helicase )

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-26
Identity = 68/205 (33.17%), Postives = 106/205 (51.71%), Query Frame = 0


Query: 126 IDNGTKPFVE-EDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNATLIEYITSRAILS 185
           + NG  P  E +D   +  D     +   + + ++    Y     +    +Y+T R IL+
Sbjct: 63  VGNGNAPTCETDDGEVVEMDTSFFLKHNGNRLQQVTKGAYVQFSVSQPNFQYLTERGILT 122

Query: 186 TTNEYVDQMYEKMIHLFPGKIEEFMS------FDEAIDNTHNYYQEEFLNSLLPNGVPPH 245
             NEYVD++   M+    G  +E++S       D    +    Y  ++LNSL    +P H
Sbjct: 123 PHNEYVDEINAYMLSQVGGDSKEYLSSYSIGKADTIGADYEALYHVKYLNSLEFPSLPKH 182

Query: 246 KLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVIYTEIAAGQHARKKVFLPRIPMS 305
           K+ LKK  P+M +RN +   GLCNGTR++     + VI  +I  G HA K V +PR  +S
Sbjct: 183 KISLKKGVPIMQMRNFNQKEGLCNGTRLIVTNLGEQVIEAQIVTGTHAGKMVSIPRFILS 242

Query: 306 PTNDEGYPFKFKRKQFPVRLCFAMT 324
           P   E +PF  +R+QFP+R+C+AMT
Sbjct: 243 PPQSE-HPFTLRRQQFPMRVCYAMT 266

[Close]

BLAST of Lcy12g005490 vs. TAIR 10
Match: AT3G51690.1 (PIF1 helicase )

HSP 1 Score: 104.0 bits (258), Expect = 2.3e-22
Identity = 73/214 (34.11%), Postives = 109/214 (50.93%), Query Frame = 0


Query: 118 TINESL------VKIDNGTKPFVEEDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNA 177
           TI+E+L      +  DN   P   E  I +  D++IT     D +  LL  VY +    +
Sbjct: 54  TIDEALSRWIMNISGDNINNPNDGETEIDISKDLLIT--ESKDPIKTLLKEVYGEYFAKS 113

Query: 178 TLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFD--EAIDNTHNYYQEEFLNS 237
              ++    AIL   ++ VDQ+ + M+ L PG+ +E +S D      N   +   E LNS
Sbjct: 114 YNPDFCHDSAILCHRDDDVDQINDYMLSLLPGEEKECLSTDSISPSPNDDMFVPLEVLNS 173

Query: 238 LLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVIYTEIAAGQHARKK 297
           +   G+P  KL LK   PVMLLR+LDP  G   GTR+        ++   I AG    KK
Sbjct: 174 IKVPGLPDFKLRLKVGAPVMLLRDLDPSRGFFTGTRLQITRLCGFLLEAMIIAGNKHGKK 233

Query: 298 VFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
           +++PRI   PT +  +P + +R Q+P++L FAMT
Sbjct: 234 IWIPRIASYPT-ETNFPLQMRRTQYPLKLAFAMT 264

[Close]

BLAST of Lcy12g005490 vs. TAIR 10
Match: AT3G51700.1 (PIF1 helicase )

HSP 1 Score: 97.4 bits (241), Expect = 2.2e-20
Identity = 73/217 (33.64%), Postives = 106/217 (48.85%), Query Frame = 0


Query: 118 TINESLVKI------DNGTKPFVEEDYICLPNDVVITSESENDAVLKLLNIVYPDLEKNA 177
           TI+E+  K       +N  KP   E  I +  D++IT     D +  +++ VY +    +
Sbjct: 55  TIDEAFTKWITNIGGENINKPNDGETKIDIHEDLLIT--ECKDPIKTIVDEVYGESFTES 114

Query: 178 TLIEYITSRAILSTTNEYVDQMYEKMIHLFPGKIEEFMSFD-----EAIDNTHNYYQEEF 237
              ++   RAIL  TN+  D++ + M+    G+  +    D      A  N    Y  EF
Sbjct: 115 YNPDFYQERAILCHTNDVADEINDYMLSQLQGEETKCYGADTIYPTHASPNDKMLYPLEF 174

Query: 238 LNSLLPNGVPPHKLILKKHCPVMLLRNLDPPNGLCNGTRMVCHEFHKNVIYTEIAAGQHA 297
           LNS+   G P  KL LK   PVMLLR+L P   L  GTR+        V+   I  G + 
Sbjct: 175 LNSIKIPGFPDFKLRLKVGAPVMLLRDLAPYGWLRKGTRLQITRVETFVLEAMIITGNNH 234

Query: 298 RKKVFLPRIPMSPTNDEGYPFKFKRKQFPVRLCFAMT 324
            +KV +PRIP S   +  +P K +R+QFPV+L FAMT
Sbjct: 235 GEKVLIPRIP-SDLREAKFPIKMRRRQFPVKLAFAMT 268

The following BLAST results are available for this feature:

BLAST of Lcy12g005490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UUA2	8.6e-06	26.87	ATP-dependent DNA helicase pfh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC ...	[more]
P38766	1.9e-05	24.03	ATP-dependent DNA helicase RRM3 OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]
A5WFR0	2.5e-05	24.64	ATP-dependent DNA helicase pif1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 G...	[more]
Q0R4F1	4.3e-05	24.63	ATP-dependent DNA helicase PIF1 OS=Xenopus laevis OX=8355 GN=pif1 PE=2 SV=1	[more]
Q7ZV90	4.7e-04	24.73	ATP-dependent DNA helicase PIF1 OS=Danio rerio OX=7955 GN=pif1 PE=2 SV=1	[more]

BLAST of Lcy12g005490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DMF2	2.0e-122	68.81	ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111022374 PE=3 S...	[more]
A0A5B7A9C3	3.6e-116	59.09	ATP-dependent DNA helicase OS=Davidia involucrata OX=16924 GN=Din_021512 PE=3 SV...	[more]
A0A5B7A5M4	2.4e-115	58.81	ATP-dependent DNA helicase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_021...	[more]
A0A6J1DFU4	2.9e-113	71.58	ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111020055 PE=3 S...	[more]
A0A1U7WW73	4.3e-101	58.68	ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104231538 PE=3 ...	[more]

BLAST of Lcy12g005490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155233.1	4.1e-122	68.81	ATP-dependent DNA helicase PIF1-like [Momordica charantia]	[more]
XP_022152307.1	6.0e-113	71.58	ATP-dependent DNA helicase PIF1-like [Momordica charantia]	[more]
XP_020242961.1	4.9e-107	54.55	uncharacterized protein LOC109821182 [Asparagus officinalis]	[more]
XP_012841137.1	1.1e-106	56.53	PREDICTED: uncharacterized protein LOC105961450 [Erythranthe guttata]	[more]
XP_020275823.1	1.9e-106	55.52	ATP-dependent DNA helicase PIF1-like [Asparagus officinalis]	[more]

BLAST of Lcy12g005490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G28780.1	2.7e-26	33.17	PIF1 helicase	[more]
AT3G51690.1	2.3e-22	34.11	PIF1 helicase	[more]
AT3G51700.1	2.2e-20	33.64	PIF1 helicase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010285	DNA helicase Pif1-like	PFAM	PF05970	PIF1	coord: 2..124 e-value: 1.0E-40 score: 140.1
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 2..127 e-value: 3.0E-11 score: 45.1
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 90..323
None	No IPR available	PANTHER	PTHR10492	UNCHARACTERIZED	coord: 1..323
None	No IPR available	PANTHER	PTHR10492:SF76	ATP-DEPENDENT DNA HELICASE	coord: 1..323

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g005490.1	Lcy12g005490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
biological_process	GO:0000723	telomere maintenance
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003678	DNA helicase activity