Lcy12g005300 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g005300
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationChr12: 20544696 .. 20549290 (-)
RNA-Seq ExpressionLcy12g005300
SyntenyLcy12g005300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGGCTTAAGGAAATGTTTGGAAACGAACGACGCGTTTGGCGTCCCAAGCAAACCCCTACGGCGTCGCATCGATATAATTTTTCAACAAAATTTCTCAAACATCACACTCTGGTTGGGTCCTATCATTCTCTCTCTCTCCATCTTGATTACCTTGATTAGGTTCAGTTTCTTCATCCCCAGTCTCGCCATTCCTTTTCGAATTCTCAACTCGATCACAGCTTTCACACTTGTCGATTCTTCCTTTCGGCTTCTTATCATCCTGCAATTCGGTGGTGAAAAGGGTTTTTGTTTTGATCTTCATTCGATCTTCATCTCCATGTCGATGCTCAACTCCTTCTTCAACAAGGGCTTCAAAGCTGCCCAATGGTATCGCTTCTGTTTATTTTGATTTTCGTTTTCTTTTCATTTTCTCTTTCCTTGCTCTTTCAATTGTGTCGGTCACTGCTTTCATTGATTCATATGCGATTACAGAATTTCTGCTTTTGATTGTAATTTGCGCTTCTGTATTCAGTAAAACCCTGCTTAAATTGACAATTCCACGAATAAAGCTGCTGAGGAATCGGAGGGAGATTCAGCTTAAGCAGATGCGACGGGACATAGCTAAGCTTCTTGAAACAGGCCAAGAAGCTACGGCTCGCATTCGGGTATGTGTTACTTTCTGAAGTGCATTTCACCAATTTAATATCAAGAGGGGGAGGATAAGAAAAAAGGGAAAAAAATTTATGTCCTGAAAGTTTTTGTTGTCTTAATTAGATCTGCTTTTGCATTCAGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTTCCAATTATTGAAACACAAAGGTAAATTTTCCTAACGAAGTTATTCTTTATATGAGGATATTAAGTGTTTCTTGTGGTTATCTAAATGAACACGAGAAAGATCTGAATGCAAATAAGTTAAATAGATTACAGTATTTTCATGGACCCTTGCTCTTCAACCTGTAGGATTTGGTTTGAAACAGACAGCTCAAAAAGTCTTCACACGATATCTAATATTTGTGAATTTTTAAAAATGACAACGATAATAATGGAGAAATTGACACTAATGGGAAATGCTATGGCTTTTGTCTACTGCTTATTTCTTCTTCTTCTTCCTAATATTTTGATTAAAAATGTCTAAATGAGTATAGCTGTCCATGTTTGGCAATTATGAAACACAATTTATTCTGGGAAACATTTATGTTTTCGATATGAACATGACTGACAAAACTAGGATGTTAGATCTTTTCAATGTTTCTAAATTTGAAATCAATACTGGACTTTTCTACAAGAGTTGTATTGTATAATGGTTTTTTTTTAGTCTTTTTATGAACATTTAATTATCTAGATTAGATATGTATATTTCTTTTGGAATTTCCTTCACAAATTGTTACCAAGAAGCTGGACCATTTCATGCCTACAACCAACCGAACTTGGAACATTGGATTTGTTTGTTGGACTTATGTACAAGTATAATTTTTTAAGTATTTTATTTACGAGGATTTAATTCTATGATTAGATGTGTATATATTTGGTTCGAGATTTCCCTCATAAATTGTGACTGAGGAGCTGCATCATATAGAAATATGTAATTGATGTCAAAGTTTCTCACTGATGTTGTATCTACAGTCCATTCTTTCCCATTATTGAATGAACTGAATCTGTCCAATTGTTTTTGTCCTTACTCCTTGTCTATGAGAAAATGGCTTGTGGCTCTCTCTTTCTTGGGTTGGGTTATTGTCATGTTTGTAAAACTCATCAGTAGTATCCATATTTGTGCTCTTTTGGTTGATAAGAATGAGCATTAGAAATATGCTGGTATTTCACTTTAAAAGTTTGAACAGATATTTGCACATAAAGATAGAATATTGGAGAATATGTGCTCCTTTGAGGAATATTTTCCCATATTGAAGAATATTCTTTCATCCTAGTTTGGGATATTGGAGATCCATCCCAAAATTATGGAAGTTAAGTCTTAGATTGAAAAATTGTTAATTATAACTACAGCTAGTTGTCCAGCCTGATCATCCAACTTGTCCTGCGAATGACTCAAAACATCAAGCCTTGCAAACTGGAAATTTAGTTGCAATTGATTATGCTTAAAGGGTGGATGCTAAACTTACAAGAAACTGATTAGTTTGCACTTTCAAATTCACAAAATTTTTGGGTTAATCACCAAGCTTTCCATTTTTGGTGAAAATCCATGTCGTCTTTTGGATAACAGCTTTTGTCCCAAGTTACCTGAAATTTAAAACTACATGTCCAAATTGTATCCTTACTGTAAAATCTTTTGAATCTTACTCCATCTTTATTGTAGAGAATGCTTGCATTTCCATGCATTTCTTCCTTTGTTCAATTGGTGCTTAATTAGTTGCAGTATCTTGATATTTCATATGGTCCCATTTTTATTTCATCCTGTCCAATGATATCTTGGTATCAATATTTATAAATACCATATATCTTTCTACGTTTTTTTTTATCATTCTGATTTCACTCTGTTGCTTATGGTACTTGTATTGAGACATTTGTTCCTTCTACTTTATGCTTGTCCCAAATATCATTGTAAACTCCCAAATTTTTCAGGGAATGTCCTCTAGACTTGAAAGAAGCTATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGCGCTGATCTTACAGAGCTTCTCCAGGTTCAGATGCATTTTGCTGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAATCGTCAGGTTCTTAGAATTTATTGTTTATGTTTGATCAGAAAGAAAGCTTTACTGCAGTCGATAGGATTATATATTTCCTTTTAATTTTGGTTGCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATTGCTGAAGAGCACGACTTAGATTGGGATCCTGCTGGAACGGAAGCTGAGTTTAACAAATCTCCTGAAGATTTGCTTGTAAGTGTCGTTCCTTTTTGTCTCAACTTGTGGTGACTCTTCATGTCTTAGTTTCTGTTCTCTCTCTTCACAGAATGGATCGGCGCAGTTTGTCAGTGCATCAAAATTACCCCTTCCTAAAGAGAAACATGACGAAACGTATAATGCTACTCCTGATTTGGCTAGTACACCACAACCTGATTCTGATTCAGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATCTCCACATCCTCCTACTGTAGAGGCTGGACCTGCATCATCACTGATCCCACCTCCATCTGTGCCACTACCGCCGCCGGAATCTGATCATGATTCATTCAAGTATTCTGGTTTTCCTGAAATCCCACAAGATTTACACCTAAGACATGATGAGGCGACATTGGTGAGATCTGTTTCTCCCAGCAACCATGAAATGAATATCTCTGTTGGTGAAGATAAACAGTTTTTGCCTTTTATTACTACCCCATCATTGTCATCTTCGTTTTCTCATAGACAAACTGATTTGTCGCCACCCTCTGAAACAAGAACTCCCGAGGAGAAGTTTGGTTTTGAACCAAAGCTCGAACCCGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCCTTTCAAACCACAGTTTGAACAGGAAATAAATTCACCACCACCCTCTGCTTCAAGGACAAAGAGTGAGGTCAATGTGGATATCAGTGTGGATTTGCAGGATGTATTGGCCGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTCTTGCAAAGGTTAGAATCGACGAGCTCACAAAGAAAAAGAACGATCATGACCCTGAAATTAATTGCGAGAATCCATTCCATGGAGCTACTGCAAGTCCACCAGACCATAAAGAAACATTCCAACTAAACCAACAAGATTCTCCGGGGCATCATTCTAGTTCTATGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAGGTAAGCCGGAATTCAGATGTTTCTGATCACCACCAGCTCGAGGAACAGAAATCGGGTTTCGATTCCTCGCCCGGTGATTCACCATCTGAACGTATAGTACATCCTCATCAACCCCAGAGGCTTCCATCAATGGATGACGACCCGTACTTTTCATACCCAAATTTATTTACATCACAGAAACCAAATCTGGGATCTGATCATTCATCTGGTGGTGGTTCCTGATCTACCCATGAGGGTTGAGTTGAATTACATGCCATGCCGTCTAGGCTGGGGCCTCGTGAATTAATATTTCGTTCAGCTTTCATAACGAGTTTTCAATATCCTCTAGAGCTATAGAAGCGAGAATTCAATGTACAAAACAGTAAAGATATATTGTACAATCAGGATTGGTCTCTTGTTATGTACATTTTTCGGCCTATAGTTTGTATTTATTTGATTATTTCAGATGTCACTAATATAATGCTATAAATCATTTTAGGGCTGGATCTAAAAAGCTCGGATCCTTCTACTGTAGTTGTTAAAATGAGGATTAACTACACAGTTAGACTATTTTCAAC

mRNA sequence

TCTGGCTTAAGGAAATGTTTGGAAACGAACGACGCGTTTGGCGTCCCAAGCAAACCCCTACGGCGTCGCATCGATATAATTTTTCAACAAAATTTCTCAAACATCACACTCTGGTTGGGTCCTATCATTCTCTCTCTCTCCATCTTGATTACCTTGATTAGGTTCAGTTTCTTCATCCCCAGTCTCGCCATTCCTTTTCGAATTCTCAACTCGATCACAGCTTTCACACTTGTCGATTCTTCCTTTCGGCTTCTTATCATCCTGCAATTCGGTGGTGAAAAGGGTTTTTGTTTTGATCTTCATTCGATCTTCATCTCCATGTCGATGCTCAACTCCTTCTTCAACAAGGGCTTCAAAGCTGCCCAATGTAAAACCCTGCTTAAATTGACAATTCCACGAATAAAGCTGCTGAGGAATCGGAGGGAGATTCAGCTTAAGCAGATGCGACGGGACATAGCTAAGCTTCTTGAAACAGGCCAAGAAGCTACGGCTCGCATTCGGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTTCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCTATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGCGCTGATCTTACAGAGCTTCTCCAGGTTCAGATGCATTTTGCTGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAATCGTCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATTGCTGAAGAGCACGACTTAGATTGGGATCCTGCTGGAACGGAAGCTGAGTTTAACAAATCTCCTGAAGATTTGCTTAATGGATCGGCGCAGTTTGTCAGTGCATCAAAATTACCCCTTCCTAAAGAGAAACATGACGAAACGTATAATGCTACTCCTGATTTGGCTAGTACACCACAACCTGATTCTGATTCAGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATCTCCACATCCTCCTACTGTAGAGGCTGGACCTGCATCATCACTGATCCCACCTCCATCTGTGCCACTACCGCCGCCGGAATCTGATCATGATTCATTCAAGTATTCTGGTTTTCCTGAAATCCCACAAGATTTACACCTAAGACATGATGAGGCGACATTGGTGAGATCTGTTTCTCCCAGCAACCATGAAATGAATATCTCTGTTGGTGAAGATAAACAGTTTTTGCCTTTTATTACTACCCCATCATTGTCATCTTCGTTTTCTCATAGACAAACTGATTTGTCGCCACCCTCTGAAACAAGAACTCCCGAGGAGAAGTTTGGTTTTGAACCAAAGCTCGAACCCGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCCTTTCAAACCACAGTTTGAACAGGAAATAAATTCACCACCACCCTCTGCTTCAAGGACAAAGAGTGAGGTCAATGTGGATATCAGTGTGGATTTGCAGGATGTATTGGCCGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTCTTGCAAAGGTTAGAATCGACGAGCTCACAAAGAAAAAGAACGATCATGACCCTGAAATTAATTGCGAGAATCCATTCCATGGAGCTACTGCAAGTCCACCAGACCATAAAGAAACATTCCAACTAAACCAACAAGATTCTCCGGGGCATCATTCTAGTTCTATGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAGGTAAGCCGGAATTCAGATGTTTCTGATCACCACCAGCTCGAGGAACAGAAATCGGGTTTCGATTCCTCGCCCGGTGATTCACCATCTGAACGTATAGTACATCCTCATCAACCCCAGAGGCTTCCATCAATGGATGACGACCCGTACTTTTCATACCCAAATTTATTTACATCACAGAAACCAAATCTGGGATCTGATCATTCATCTGGTGGTGGTTCCTGATCTACCCATGAGGGTTGAGTTGAATTACATGCCATGCCGTCTAGGCTGGGGCCTCGTGAATTAATATTTCGTTCAGCTTTCATAACGAGTTTTCAATATCCTCTAGAGCTATAGAAGCGAGAATTCAATGTACAAAACAGTAAAGATATATTGTACAATCAGGATTGGTCTCTTGTTATGTACATTTTTCGGCCTATAGTTTGTATTTATTTGATTATTTCAGATGTCACTAATATAATGCTATAAATCATTTTAGGGCTGGATCTAAAAAGCTCGGATCCTTCTACTGTAGTTGTTAAAATGAGGATTAACTACACAGTTAGACTATTTTCAAC

Coding sequence (CDS)

ATGTCGATGCTCAACTCCTTCTTCAACAAGGGCTTCAAAGCTGCCCAATGTAAAACCCTGCTTAAATTGACAATTCCACGAATAAAGCTGCTGAGGAATCGGAGGGAGATTCAGCTTAAGCAGATGCGACGGGACATAGCTAAGCTTCTTGAAACAGGCCAAGAAGCTACGGCTCGCATTCGGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTTCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCTATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGCGCTGATCTTACAGAGCTTCTCCAGGTTCAGATGCATTTTGCTGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAATCGTCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATTGCTGAAGAGCACGACTTAGATTGGGATCCTGCTGGAACGGAAGCTGAGTTTAACAAATCTCCTGAAGATTTGCTTAATGGATCGGCGCAGTTTGTCAGTGCATCAAAATTACCCCTTCCTAAAGAGAAACATGACGAAACGTATAATGCTACTCCTGATTTGGCTAGTACACCACAACCTGATTCTGATTCAGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATCTCCACATCCTCCTACTGTAGAGGCTGGACCTGCATCATCACTGATCCCACCTCCATCTGTGCCACTACCGCCGCCGGAATCTGATCATGATTCATTCAAGTATTCTGGTTTTCCTGAAATCCCACAAGATTTACACCTAAGACATGATGAGGCGACATTGGTGAGATCTGTTTCTCCCAGCAACCATGAAATGAATATCTCTGTTGGTGAAGATAAACAGTTTTTGCCTTTTATTACTACCCCATCATTGTCATCTTCGTTTTCTCATAGACAAACTGATTTGTCGCCACCCTCTGAAACAAGAACTCCCGAGGAGAAGTTTGGTTTTGAACCAAAGCTCGAACCCGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCCTTTCAAACCACAGTTTGAACAGGAAATAAATTCACCACCACCCTCTGCTTCAAGGACAAAGAGTGAGGTCAATGTGGATATCAGTGTGGATTTGCAGGATGTATTGGCCGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTCTTGCAAAGGTTAGAATCGACGAGCTCACAAAGAAAAAGAACGATCATGACCCTGAAATTAATTGCGAGAATCCATTCCATGGAGCTACTGCAAGTCCACCAGACCATAAAGAAACATTCCAACTAAACCAACAAGATTCTCCGGGGCATCATTCTAGTTCTATGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAGGTAAGCCGGAATTCAGATGTTTCTGATCACCACCAGCTCGAGGAACAGAAATCGGGTTTCGATTCCTCGCCCGGTGATTCACCATCTGAACGTATAGTACATCCTCATCAACCCCAGAGGCTTCCATCAATGGATGACGACCCGTACTTTTCATACCCAAATTTATTTACATCACAGAAACCAAATCTGGGATCTGATCATTCATCTGGTGGTGGTTCCTGA

Protein sequence

MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEIPQDLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINSPPPSVSRTPFKPQFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS
Homology
BLAST of Lcy12g005300 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.4e-21
Identity = 66/176 (37.50%), Postives = 107/176 (60.80%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY KE+            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Lcy12g005300 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.4e-21
Identity = 66/176 (37.50%), Postives = 107/176 (60.80%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY KE+            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Lcy12g005300 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.7e-20
Identity = 65/176 (36.93%), Postives = 106/176 (60.23%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           G KA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY KE+            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Lcy12g005300 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.2e-20
Identity = 62/180 (34.44%), Postives = 106/180 (58.89%), Query Frame = 0

Query: 8   FNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIR 67
           F   + + + K  LKL + RI++L+N++   ++  +R++A+LL    E +ARIRVE IIR
Sbjct: 4   FGPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIR 63

Query: 68  EENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQM 127
           +E ++   +I+E+ CEL+  R+ +I    E PL++KE+I ++ +++ R   + EL Q++ 
Sbjct: 64  DEYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQR-IQIPELEQIKN 123

Query: 128 HFAAKYGKEFVSAATELMPNC----GVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW 184
              AKYGK   + A     NC     VN +++  LS   P P    + L EIAE+ ++DW
Sbjct: 124 QLKAKYGKGLENEA-----NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 177

BLAST of Lcy12g005300 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.2e-20
Identity = 65/176 (36.93%), Postives = 106/176 (60.23%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY K +            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKGYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Lcy12g005300 vs. ExPASy TrEMBL
Match: A0A6J1J0W1 (uncharacterized protein LOC111480885 OS=Cucurbita maxima OX=3661 GN=LOC111480885 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 4.2e-264
Identity = 509/615 (82.76%), Postives = 535/615 (86.99%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQ+ FAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQ 300
           LD LDFPEVPK+SVS HPPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQ
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVS-PPPETDCDSFKSSGIIPEIPPQ 300

Query: 301 DLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRT 360
           DLHLRH+E   VRSVSPSN  +NISVGEDKQFLPFIT PSLSSSFSHRQTDL P S+++T
Sbjct: 301 DLHLRHEE---VRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKT 360

Query: 361 PEEKFGFEPKLEPEINSPPPSVSRT-------------------------------PFKP 420
           PEEKFGF+P+ E EINSPP SVSRT                                FKP
Sbjct: 361 PEEKFGFKPRFEQEINSPPLSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKP 420

Query: 421 QFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480
           QFEQ ++SPPPS SRTKSEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI
Sbjct: 421 QFEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480

Query: 481 DELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEH 540
           DELTKKKND D E++CENPFHGA  +PPDHK+TF   QQDS G +  SMPYYSQDSF+EH
Sbjct: 481 DELTKKKNDRDAEVSCENPFHGAAVTPPDHKQTF---QQDSLGDY--SMPYYSQDSFKEH 540

Query: 541 HKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTS 582
           H+VS +SDV+ HHQLE QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTS
Sbjct: 541 HRVSCDSDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTS 600

BLAST of Lcy12g005300 vs. ExPASy TrEMBL
Match: A0A6J1EEM3 (uncharacterized protein LOC111433438 OS=Cucurbita moschata OX=3662 GN=LOC111433438 PE=3 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 2.0e-261
Identity = 502/615 (81.63%), Postives = 534/615 (86.83%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQ+ FAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQ 300
           LD LDFPEVPK+SVS HPPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQ
Sbjct: 241 LDPLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQ 300

Query: 301 DLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRT 360
           DLHL+ +E   VRSVSPSN  +N+SVGEDKQFLPFIT PSLSSSFSHRQTD+ P S+++T
Sbjct: 301 DLHLKREE---VRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKT 360

Query: 361 PEEKFGFEPKLEPEINSPPPSVSRT-------------------------------PFKP 420
           PEEKFGF+P+ E EINSPPPSVSRT                                FKP
Sbjct: 361 PEEKFGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEETNSLRPSVSPEEKFGFKP 420

Query: 421 QFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480
           QFEQ ++SPPPS SRTKSEVNVD+SV+LQDVLAAAQAAAETAERAAAAARSAASLAKVRI
Sbjct: 421 QFEQNLDSPPPSVSRTKSEVNVDVSVNLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480

Query: 481 DELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEH 540
           DELTKKKND D E++CENPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EH
Sbjct: 481 DELTKKKNDRDAEVSCENPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEH 540

Query: 541 HKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTS 582
           H+VS + DV+ HHQLE QKSGFDSSP +SPSERI HPHQ QRLPSMDDDPYFSYPNLFTS
Sbjct: 541 HRVSCDLDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTS 600

BLAST of Lcy12g005300 vs. ExPASy TrEMBL
Match: A0A0A0KBZ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 7.0e-259
Identity = 490/588 (83.33%), Postives = 523/588 (88.95%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL+QVQM F AKYGKEF+SAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDW+PA TEAEFNKSPEDLLNGS QFV ASKLPLP+EKH+ET N T DLAS PQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDL 300
           LDMLDFPEVPKMSV P  PT++AG A S+IPPPSV  PP E+DH SF+YSG PE  PQ+L
Sbjct: 241 LDMLDFPEVPKMSVCPQ-PTIDAGSAPSMIPPPSVS-PPRETDHASFEYSGIPESPPQNL 300

Query: 301 HLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPE 360
           H RH+E TLVRSVSPSN +MN+SVGEDKQFLPFIT PSLS SFS RQT+LSP S+  TPE
Sbjct: 301 HSRHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPE 360

Query: 361 EKFGFEPKLEPEINSPPPSVSRTP-----FKPQFEQEINSPPPSASRTKSEVNVDISVDL 420
           EKF  +P++  EINSP PSVSR       FKP+FE EINS P S SRT SEVN D+ VDL
Sbjct: 361 EKFDIKPRIREEINSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDL 420

Query: 421 QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPP 480
           QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND DPEI+CENPFHG TASP 
Sbjct: 421 QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPA 480

Query: 481 DHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGD 540
           DH ET++LNQQDS G+HSS+MPYYSQDSFQEHHK+SR+ +VSDH QL+ +KS FD SPG+
Sbjct: 481 DHTETYKLNQQDSLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGN 540

Query: 541 SPSE-RIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 582
           SPSE + V PHQPQRLPSMDDDPYFSYPNLFTSQKPN G DHSS GGS
Sbjct: 541 SPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 586

BLAST of Lcy12g005300 vs. ExPASy TrEMBL
Match: A0A1S4E4M8 (uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 5.0e-257
Identity = 492/588 (83.67%), Postives = 519/588 (88.27%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL+QVQM F AKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELIQVQMLFGAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDW+PA TEAEFNKSPEDLLNG   FV  SKLPLP+EKH+ET N T DLAS PQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNG---FVGTSKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDL 300
           LDMLDFPEVPKMSV P  PT++AG A S+I PPSV  P PE+D  SFKYSG PE  PQ+L
Sbjct: 241 LDMLDFPEVPKMSVCPQ-PTIDAGSAPSMILPPSVS-PQPETDRASFKYSGIPESPPQNL 300

Query: 301 HLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPE 360
           H RH E TLVRSVSPSN +MN+SVGEDKQFLPFIT PSLS SFSHRQTDLSPPS+  TPE
Sbjct: 301 HSRHGEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSHRQTDLSPPSDPTTPE 360

Query: 361 EKFGFEPKLEPEINSPPPSVSRTP-----FKPQFEQEINSPPPSASRTKSEVNVDISVDL 420
           EKF  +P++  EINSP PSVSR       FKP+F+ EINS P S SRT  EVN D+SVDL
Sbjct: 361 EKFDIKPRITEEINSPLPSVSRASEEQFGFKPRFDSEINSTPSSVSRTMCEVNADLSVDL 420

Query: 421 QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPP 480
           QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND DPEI+CENPFHGATASP 
Sbjct: 421 QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGATASPA 480

Query: 481 DHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGD 540
            H ET++LNQQDS GHHSSSMPYYSQDS QEHHK+SR+ +VSDH QLE +KS FDSSPG+
Sbjct: 481 GHTETYKLNQQDSLGHHSSSMPYYSQDSSQEHHKLSRDPEVSDHSQLEPEKSSFDSSPGN 540

Query: 541 SPSE-RIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 582
           SPSE + V PHQPQRLPSMDDDPYFSYPNLFTSQKPN G DHSS GGS
Sbjct: 541 SPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 583

BLAST of Lcy12g005300 vs. ExPASy TrEMBL
Match: A0A6J1EU69 (uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436655 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.3e-244
Identity = 476/586 (81.23%), Postives = 497/586 (84.81%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML SFF KGFK AQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLASFFYKGFKGAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQ+ FA KYGKEFVSAATELMPNCGVNRQL+ELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAVKYGKEFVSAATELMPNCGVNRQLVELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           +DWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLP+EKH+E YNA       PQPDSDSE
Sbjct: 181 VDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNA-------PQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDL 300
           LDMLDFPEVPK+SVSPH  T++ G ASS+IPPPS   PPPE+ HDS KYS  PE  P+DL
Sbjct: 241 LDMLDFPEVPKISVSPH-STIDPGSASSMIPPPSTS-PPPETTHDSSKYSCIPETPPEDL 300

Query: 301 HLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPE 360
            LR  E T VRSVSPS  +MNISVGEDKQFLPFI  PS                  RTPE
Sbjct: 301 QLRRGEVTPVRSVSPSKDQMNISVGEDKQFLPFIAPPS------------------RTPE 360

Query: 361 EKFGFEPKLEPEINSPPPSVSRTP---FKPQFEQEINSPPPSASRTKSEVNVDISVDLQD 420
           EKF F+P+ EPEINSPPPSVSRTP   F  ++EQEI SPPPSASRTKS    D+S+DLQD
Sbjct: 361 EKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEIISPPPSASRTKS----DVSLDLQD 420

Query: 421 VLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPPDH 480
           VLAAAQAA ETAERAAAAARSAASL KVRID LT K ND  PEI CENPFHGAT SPPDH
Sbjct: 421 VLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQGPEICCENPFHGATTSPPDH 480

Query: 481 KETFQLNQQDSPG-HHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGDS 540
           ++TF LNQQDS G HHSSSMPYYSQDSFQEHHK S  SDVSDHH+ E QKSGFDSSPGDS
Sbjct: 481 EQTFHLNQQDSIGHHHSSSMPYYSQDSFQEHHKESPGSDVSDHHRFESQKSGFDSSPGDS 540

Query: 541 PSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 582
           PSE I  PHQPQRLPSMDDDPYFSYPNLFTSQKPN G DHSS  GS
Sbjct: 541 PSEHITIPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSADGS 555

BLAST of Lcy12g005300 vs. NCBI nr
Match: XP_038884985.1 (uncharacterized protein LOC120075564 [Benincasa hispida])

HSP 1 Score: 926.0 bits (2392), Expect = 1.6e-265
Identity = 501/587 (85.35%), Postives = 527/587 (89.78%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQM FAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAE+H+
Sbjct: 121 ELLQVQMLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEDHN 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDW+PA TEAEFNKSPEDLLNGSAQFVSASKLPLP+E H+E YNATPDLAS+PQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNGSAQFVSASKLPLPQENHNEAYNATPDLASSPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDL 300
           LDMLDFPEVPK+SV P P T +AG ASS++PPPSVP P  E++H S KYS  PE  PQDL
Sbjct: 241 LDMLDFPEVPKISVCPQPNT-DAGSASSMMPPPSVP-PASETNHASLKYSDIPETPPQDL 300

Query: 301 HLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPE 360
           HLRH+E T VRSVSPS  +MNISVGEDKQFLPFIT PSLSSSFSHRQTD  PPS++  PE
Sbjct: 301 HLRHEEVTSVRSVSPSTDQMNISVGEDKQFLPFITPPSLSSSFSHRQTDFLPPSDSTAPE 360

Query: 361 EKFGFEPKLEPEINSPPPSVSRTP-----FKPQFEQEINSPPPSASRTKSEVNVDISVDL 420
           EKFGF+P++E EINSP PSVSRT      FKP+FE EIN+PP S SRT SEVN D+ VDL
Sbjct: 361 EKFGFKPRIEQEINSPLPSVSRTAEEKFGFKPRFEPEINAPPSSVSRTMSEVNEDVGVDL 420

Query: 421 QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPP 480
           QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND DPEI+CENPFHGATAS P
Sbjct: 421 QDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGATASSP 480

Query: 481 DHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGD 540
           DHKETFQLNQQD  GHHSSSMPYYSQDSFQE H VS + DVS+HHQ E +K GFDSSPGD
Sbjct: 481 DHKETFQLNQQDYLGHHSSSMPYYSQDSFQERHIVSHSLDVSEHHQPEPEKLGFDSSPGD 540

Query: 541 SPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 582
           SPS  I  PHQPQRLPSMDDDPYFSYPNLFT QKPN GSDH S GGS
Sbjct: 541 SPSAHIAVPHQPQRLPSMDDDPYFSYPNLFTPQKPNPGSDHPSAGGS 585

BLAST of Lcy12g005300 vs. NCBI nr
Match: XP_023521005.1 (uncharacterized protein LOC111784587 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.8e-264
Identity = 508/615 (82.60%), Postives = 536/615 (87.15%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQ+ FAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQ 300
           LD LDFPEVPK+SVS HPPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQ
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQ 300

Query: 301 DLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRT 360
           DLHLRH+E   VRSVSPSN  +NISVGEDKQFLPFIT PSLSSSFSHRQTDL P S+++T
Sbjct: 301 DLHLRHEE---VRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKT 360

Query: 361 PEEKFGFEPKLEPEINSPPPSVSRT-------------------------------PFKP 420
           PEEK GF+P+ E EINSPPPSVSRT                                FKP
Sbjct: 361 PEEKLGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKP 420

Query: 421 QFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480
           QFEQ ++SPPPS SRTKSEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI
Sbjct: 421 QFEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480

Query: 481 DELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEH 540
           DELTKKKND D E++CENPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EH
Sbjct: 481 DELTKKKNDRDAEVSCENPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEH 540

Query: 541 HKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTS 582
           H+VS +SDV+ HHQLE QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTS
Sbjct: 541 HRVSCDSDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTS 600

BLAST of Lcy12g005300 vs. NCBI nr
Match: XP_022981875.1 (uncharacterized protein LOC111480885 [Cucurbita maxima])

HSP 1 Score: 920.2 bits (2377), Expect = 8.7e-264
Identity = 509/615 (82.76%), Postives = 535/615 (86.99%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQ+ FAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQ 300
           LD LDFPEVPK+SVS HPPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQ
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVS-PPPETDCDSFKSSGIIPEIPPQ 300

Query: 301 DLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRT 360
           DLHLRH+E   VRSVSPSN  +NISVGEDKQFLPFIT PSLSSSFSHRQTDL P S+++T
Sbjct: 301 DLHLRHEE---VRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKT 360

Query: 361 PEEKFGFEPKLEPEINSPPPSVSRT-------------------------------PFKP 420
           PEEKFGF+P+ E EINSPP SVSRT                                FKP
Sbjct: 361 PEEKFGFKPRFEQEINSPPLSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKP 420

Query: 421 QFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480
           QFEQ ++SPPPS SRTKSEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI
Sbjct: 421 QFEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480

Query: 481 DELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEH 540
           DELTKKKND D E++CENPFHGA  +PPDHK+TF   QQDS G +  SMPYYSQDSF+EH
Sbjct: 481 DELTKKKNDRDAEVSCENPFHGAAVTPPDHKQTF---QQDSLGDY--SMPYYSQDSFKEH 540

Query: 541 HKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTS 582
           H+VS +SDV+ HHQLE QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTS
Sbjct: 541 HRVSCDSDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTS 600

BLAST of Lcy12g005300 vs. NCBI nr
Match: KAG6607619.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 917.1 bits (2369), Expect = 7.4e-263
Identity = 506/615 (82.28%), Postives = 534/615 (86.83%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQ+ FAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQ 300
           LD LDFPEVPK+SVS HPPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQ
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQ 300

Query: 301 DLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRT 360
           DLHLRH+E   VRSVSPSN  +NISVGEDKQFLPFIT PSLSSS SHRQTDL P S+++T
Sbjct: 301 DLHLRHEE---VRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSSSHRQTDLCPSSDSKT 360

Query: 361 PEEKFGFEPKLEPEINSPPPSVSRT-------------------------------PFKP 420
           PEEK GF+P+ E EINSPPPSVSRT                                FKP
Sbjct: 361 PEEKLGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKP 420

Query: 421 QFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480
           QFEQ ++SPPPS SR KSEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI
Sbjct: 421 QFEQNLDSPPPSVSRIKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480

Query: 481 DELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEH 540
           DELTKKKND D E++CENPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EH
Sbjct: 481 DELTKKKNDRDAEVSCENPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEH 540

Query: 541 HKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTS 582
           H+VS +SDV+ HHQLE QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTS
Sbjct: 541 HRVSCDSDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTS 600

BLAST of Lcy12g005300 vs. NCBI nr
Match: XP_022926259.1 (uncharacterized protein LOC111433438 [Cucurbita moschata])

HSP 1 Score: 911.4 bits (2354), Expect = 4.0e-261
Identity = 502/615 (81.63%), Postives = 534/615 (86.83%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQ+ FAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQ 300
           LD LDFPEVPK+SVS HPPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQ
Sbjct: 241 LDPLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQ 300

Query: 301 DLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRT 360
           DLHL+ +E   VRSVSPSN  +N+SVGEDKQFLPFIT PSLSSSFSHRQTD+ P S+++T
Sbjct: 301 DLHLKREE---VRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKT 360

Query: 361 PEEKFGFEPKLEPEINSPPPSVSRT-------------------------------PFKP 420
           PEEKFGF+P+ E EINSPPPSVSRT                                FKP
Sbjct: 361 PEEKFGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEETNSLRPSVSPEEKFGFKP 420

Query: 421 QFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480
           QFEQ ++SPPPS SRTKSEVNVD+SV+LQDVLAAAQAAAETAERAAAAARSAASLAKVRI
Sbjct: 421 QFEQNLDSPPPSVSRTKSEVNVDVSVNLQDVLAAAQAAAETAERAAAAARSAASLAKVRI 480

Query: 481 DELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEH 540
           DELTKKKND D E++CENPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EH
Sbjct: 481 DELTKKKNDRDAEVSCENPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEH 540

Query: 541 HKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTS 582
           H+VS + DV+ HHQLE QKSGFDSSP +SPSERI HPHQ QRLPSMDDDPYFSYPNLFTS
Sbjct: 541 HRVSCDLDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTS 600

BLAST of Lcy12g005300 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 427.6 bits (1098), Expect = 1.6e-119
Identity = 318/672 (47.32%), Postives = 383/672 (56.99%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFNKGFKAA+CKTLLKLTIPRIKL+RNRRE Q+KQMRR+IAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREE MMAAQEILELFCELI VRLPIIE QRECPLDLKEAISSVCFAAPRC+DLT
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL QVQ+ F +KYGKEFV+AA+EL P+ GVNR+L+ELLSVRAPSPE KLKLLKEIAEEH+
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHE 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSE 240
           LDWDPA TE +  KS EDLL+G  QF   SKLPLP+E++++T N T   A+  + DSDSE
Sbjct: 181 LDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKT-NLTSLSAAKEKSDSDSE 240

Query: 241 LDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEIPQDLH 300
            D+LDFPEVP + + P P       A+S+  P            D+ K + +     DL 
Sbjct: 241 YDILDFPEVPNVLLRPTP------GATSVNAP------------DAAKSASYEHTSHDLP 300

Query: 301 LRHDEATLVRSVSPSNH---EMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRT 360
              + A + ++ S  +    + + +V E +Q     ++P L  SF  +            
Sbjct: 301 FDSENAGVEKTASKRDEHPAKASKTVVEGQQ-----SSPILMESFEKK------------ 360

Query: 361 PEEKFGFEPKLEPEINSPPPSVSRTPFKPQFEQEINSPPPSASRTKSEVNVDISVDLQDV 420
                          N  PPS+      P  E         ASR       D   DLQDV
Sbjct: 361 ---------------NYSPPSIDAVGPIPTKES-------GASRDTPRKISD--GDLQDV 420

Query: 421 LAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATAS--PPD 480
           L AAQAAA++AERAA+AARSAASLA++RI+ELT+K +D  PE   ENPFH  +      D
Sbjct: 421 LMAAQAAADSAERAASAARSAASLAQLRINELTRKTSDQYPESPSENPFHAPSMGNLQFD 480

Query: 481 HK----------------ETFQL-----NQQDSPGHHSSSMP------------------ 540
           H+                ET  L     N Q    H S   P                  
Sbjct: 481 HQNSSASSSGDLTELQRAETSSLFNSEQNNQQPQTHSSMEKPQFDRQNSSFSSYGDLTPQ 540

Query: 541 -YYSQDSFQEHHKVSRNS-----------------------------DVSDHHQLEEQKS 582
            ++S +  Q  H+ S  S                             D++ H     +K 
Sbjct: 541 RFHSMEKPQFDHQNSSGSNYDDLTPQRFSSLEKPQFDHQNSSGSNYDDLTPHRFPSMEKP 600

BLAST of Lcy12g005300 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 413.3 bits (1061), Expect = 3.2e-115
Identity = 319/702 (45.44%), Postives = 383/702 (54.56%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFNKGFKAA+CKTLLKLTIPRIKL+RNRRE Q+KQMRR+IAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREE MMAAQEILELFCELI VRLPIIE QRECPLDLKEAISSVCFAAPRC+DLT
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNR---------------------------- 180
           EL QVQ+ F +KYGKEFV+AA+EL P+ GVNR                            
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNS 180

Query: 181 --QLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPEDLLNGSAQFVSAS 240
             QL+ELLSVRAPSPE KLKLLKEIAEEH+LDWDPA TE +  KS EDLL+G  QF   S
Sbjct: 181 ILQLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGS 240

Query: 241 KLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPHPPTVEAGPASSLI 300
           KLPLP+E++++T N T   A+  + DSDSE D+LDFPEVP + + P P       A+S+ 
Sbjct: 241 KLPLPEEQNEKT-NLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPTP------GATSVN 300

Query: 301 PPPSVPLPPPESDHDSFKYSGFPEIPQDLHLRHDEATLVRSVSPSNH---EMNISVGEDK 360
            P            D+ K + +     DL    + A + ++ S  +    + + +V E +
Sbjct: 301 AP------------DAAKSASYEHTSHDLPFDSENAGVEKTASKRDEHPAKASKTVVEGQ 360

Query: 361 QFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINSPPPSVSRTPFKPQ 420
           Q     ++P L  SF  +                           N  PPS+      P 
Sbjct: 361 Q-----SSPILMESFEKK---------------------------NYSPPSIDAVGPIPT 420

Query: 421 FEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID 480
            E         ASR       D   DLQDVL AAQAAA++AERAA+AARSAASLA++RI+
Sbjct: 421 KES-------GASRDTPRKISD--GDLQDVLMAAQAAADSAERAASAARSAASLAQLRIN 480

Query: 481 ELTKKKNDHDPEINCENPFHGATAS--PPDHK----------------ETFQL-----NQ 540
           ELT+K +D  PE   ENPFH  +      DH+                ET  L     N 
Sbjct: 481 ELTRKTSDQYPESPSENPFHAPSMGNLQFDHQNSSASSSGDLTELQRAETSSLFNSEQNN 540

Query: 541 QDSPGHHSSSMP-------------------YYSQDSFQEHHKVSRNS------------ 582
           Q    H S   P                   ++S +  Q  H+ S  S            
Sbjct: 541 QQPQTHSSMEKPQFDRQNSSFSSYGDLTPQRFHSMEKPQFDHQNSSGSNYDDLTPQRFSS 600

BLAST of Lcy12g005300 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 246.5 bits (628), Expect = 5.2e-65
Identity = 126/221 (57.01%), Postives = 169/221 (76.47%), Query Frame = 0

Query: 6   SFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHI 65
           S F +GF +++CKT  K+ + RIKL+RN+R + +KQMRRDIA LL++GQ+ATARIRVEH+
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 66  IREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQV 125
           IRE+N+ AA EI+ELFCELIV RL II  Q++CP+DLKE I+S+ FAAPRC+++ EL  +
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 126 QMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 185
           +  FA KYGK+FVSAAT+L P+CGVNR LI+ LSVR P  E KLK++KEIA+E  +DWD 
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDT 199

Query: 186 AGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNAT 227
             TE E  K  E+ ++G  +FVSAS LP+ +   +E  + T
Sbjct: 200 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPT 240

BLAST of Lcy12g005300 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.9e-62
Identity = 118/205 (57.56%), Postives = 160/205 (78.05%), Query Frame = 0

Query: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MS+LN  FN+G   A+CKT L L I R+KLL+N+R++QLK M+++IA  L+ GQE  ARI
Sbjct: 1   MSLLNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEH+IRE N+ AA EILELFCE I+ R+PI+E+++ECP +L+EAI+S+ FAAPRC+++ 
Sbjct: 61  RVEHVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVP 120

Query: 121 ELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           +LLQ++  F  KYGKEF+  A+EL P+ GVNR +IE LS  +PS   +LK+LKEIA+E+ 
Sbjct: 121 DLLQIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYS 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQ 206
           L+WD + TEAEF KS EDLL G+ Q
Sbjct: 181 LNWDSSATEAEFMKSHEDLLGGAKQ 205

BLAST of Lcy12g005300 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 203.0 bits (515), Expect = 6.6e-52
Identity = 101/209 (48.33%), Postives = 153/209 (73.21%), Query Frame = 0

Query: 4   LNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVE 63
           +     +GFK A+CKT L++   R+K+L+N++EIQ+KQ+RR++A+LLE+GQ  TARIRVE
Sbjct: 1   MKKVLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVE 60

Query: 64  HIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELL 123
           H++REE  +AA E++ ++CEL+VVRL +IE+Q+ CP+DLKEA++SV FA+ R +D+ EL 
Sbjct: 61  HVVREEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELS 120

Query: 124 QVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW 183
           ++   F  KYGK+F ++A EL P+ GV+R L+E LS +AP    K+K+L  IAEEH++ W
Sbjct: 121 EIFKQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVW 180

Query: 184 DPAGTEAEFNKSPEDLLNGSAQFVSASKL 213
           + A +  E +    +LLNG+  F  AS +
Sbjct: 181 E-AQSFVESDPKDTELLNGANSFQPASSM 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P539903.4e-2137.50IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX003.4e-2137.50IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q3ZBV11.7e-2036.93IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
Q54I392.2e-2034.44IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Q5R6G82.2e-2036.93IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J0W14.2e-26482.76uncharacterized protein LOC111480885 OS=Cucurbita maxima OX=3661 GN=LOC111480885... [more]
A0A6J1EEM32.0e-26181.63uncharacterized protein LOC111433438 OS=Cucurbita moschata OX=3662 GN=LOC1114334... [more]
A0A0A0KBZ07.0e-25983.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1[more]
A0A1S4E4M85.0e-25783.67uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=... [more]
A0A6J1EU691.3e-24481.23uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_038884985.11.6e-26585.35uncharacterized protein LOC120075564 [Benincasa hispida][more]
XP_023521005.11.8e-26482.60uncharacterized protein LOC111784587 [Cucurbita pepo subsp. pepo][more]
XP_022981875.18.7e-26482.76uncharacterized protein LOC111480885 [Cucurbita maxima][more]
KAG6607619.17.4e-26382.28IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022926259.14.0e-26181.63uncharacterized protein LOC111433438 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G34220.21.6e-11947.32Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.13.2e-11545.44Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.15.2e-6557.01Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.11.9e-6257.56Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.16.6e-5248.33Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 418..438
NoneNo IPR availableCOILSCoilCoilcoord: 32..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 476..503
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 450..581
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 336..353
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..294
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..280
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 336..405
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 504..525
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 383..405
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 560..581
NoneNo IPR availablePANTHERPTHR12161:SF62REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 1..574
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 18..181
e-value: 7.7E-59
score: 198.3
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 1..574
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 5..192
e-value: 6.4E-78
score: 262.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g005300.1Lcy12g005300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
molecular_function GO:0003676 nucleic acid binding