Lcy12g003330 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g003330
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionReverse transcriptase domain-containing protein
LocationChr12: 10161264 .. 10163653 (+)
RNA-Seq ExpressionLcy12g003330
SyntenyLcy12g003330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAATTAATGCTGAAGCAGAACTCGTATTGGCGTGGCACACTTTACCTTGTGCATCGTTATTGTTAATTAAGAAAAATTATAGGAACGTAAATGAGTATTAAAAATATATGGTAAATAACTCAACTAATTAACAACGTAAAAGAAATGTAAAAGAAGTATAAACATATGCCAACACAAATATAGTTGAACTGACATGCATAAAGCTTATATTAATAATCAAAAGTATGTGGTTCAGATCTCTCTATCCCACATGTTATATCTCTCTATCCCACATGTTATAAATAAGCGAAGTATAAACATAAAAGTATAATCATGATGTCATAAAATAGATGGAACTTAAAAGCTTAACATTATCATAGCTGTTGTGGGTGATTCTGGACCACCCCAGAATCCAAGGACTAAGCAGGACAACGCAGCACCAACACGAAAGCTTGACCGGGAAGTGGACCCCAGAGGAGAGAGAACCAAAGGGATTGGGTCGTCGACCCAATCCCATGCTCGGCCTCAGCCTTGGGCCGAGGCTGACCACTCGGCCCACTTGTGTGGGCCGAGTTTCTTCTACCTCTGTTCGGTCCCTGTTGCCTCGATCTGCTCCAGTTCCACTCGGTTCAGCCCATGATCGCCTCCGAGCGCCTAGAAAACCCTAATTGAAGACACATGTATAAATGGGGTCTCCCACACTCAAAAAAGGGTCGGAAAATTAGCTACCAAAAACACTCTTTACTGTTTGATCTGACTTAGGCATCGGAGTGCAGGTGGCCTACACCACAACGGTGTGTAGCGGTTCTGACTGGTTTTGCAGGTCACGTCTTCTCCGTCTTCAACAAATTTACTGCTGGTGTCACGTGAATGTCAGGTGAGTGGTTTGGCCAGACTTTGGTATCAACAATAGCGTTGTCAAATTAAAACAATCAACCATCCCAAAACTTGATTAACCAGTCAATGATGGTATTTGGCAAGATTTGTGAATCTACTCTGAGTCTCTGACTCATGGCAATAGTGCTCAACCAATGGAGTGACCCATAGTCCAATCCAATTGATGGTAGTACGTAGTATTCAATTTATAGTGCAATATAGCACACAATGAAGCACTTCAAGAGTTCATAAACATAATTTCTGTCCACAGTTGGTGCAAACAATAACTCAACTCATGGTAATTGTTACCCCCATCCGTTGTATAAGCCATCAAGAATGCATTTTATTATAAACATTTTTTCATAAAAATGATTTAGAATTTAAACACTCATATGTCTGGTTACATATTCCTCATAAGTGTTTTAAATTTATACCCAAATTATTATATACTTTTTTAAAATTTTGGTTTCATGTGTTTCTCTCTCAAAATGGTTTATTTCTCAATCTCATTTTTTAATAAGTTATTTTTAGTGGTTGTCAAACACTAAATTTTTTTTCAAAATGATTTTTTTTTTCAAAGTTAAACACTTGAAAAGTTAAACCAAACACAACATTAATATCATTGATCAAGCGACTAAGTGAGGTAATTACCACTCCCTTTTTCATGAAAACTAAACATGTTTATGATCGTACACATATGCATGAAGCCCCAATAAAATACACATGATTTTTACATGTTATACAGGAAGAAAAACGTAACATACATCAATTCAACTATATATAATTCAATCATACAATTAAAATTTAAATCCTTTGCGAGTCATGTGACTAAACATATTCCTATGTGAGAGGAACGAATGAGATGAATGATTCCTTGTCTTAGCCGTTTACACAAGATGCATATGTGGGGTAATTTGAGCGATTAAACAGCTTGATCATCTAATTAAAGCCCCAAGCCCGAATGTTTTTTTTTGCTTTGTTCTATAAAAAATCTGAGAATATCGTGTACCTAAATACTATAGAAAGTTGTTTGGCTATCTTAAATGGAAATGCTTCGTTTCAAGATTGGAACAAAACAAATATCGTTTTGATCCCTAAGGTGTGTGATCCAAAGTTGGTGACTAATTTCCTTCCTATTAGTTTGTGTAACGTATATCCTGCAAGATTATCTCAAAGGTGGTACTGCTGGCAAATAGACTCAAATCAATTCTAGATACCATTATCCCGGAAATCCCATTCGACTTTTATCAAGGTCATTCAATTTGTGATAACCTAATAATAGGTCATGAATGTCTCCATTTATTTGAAGAAATGTCGTAAAGGGAAAGTGAGATATGCGGCCTTAAAATTAGATATGAGCAAAGCATATAATAGAGTAGAATGGTCTTTCATATGTCATGTTACGAGTAAGTTGGAATTTCGAGAAGAGTGGATTGAGCTGGTAATGAATTGTATTACTATTGTCTCTTTCTCGATTCTTATCAACAACAAATCTAAGGGCTCAATTTGTCCAACCAAAGGTCTCGGATAG

mRNA sequence

ATGGAAATTAATGCTGAAGCAGAACTCGTATTGGCGTGGCACACTTTACCTTGTGCATCGTTATTGTTAATTAAGAAAAATTATAGGAACGTCATGAATGTCTCCATTTATTTGAAGAAATGTCGTAAAGGGAAAGTGAGATATGCGGCCTTAAAATTAGATATGAGCAAAGCATATAATAGAGTAGAATGGTCTTTCATATGTCATGTTACGAGTAAGTTGGAATTTCGAGAAGAGTGGATTGAGCTGGTAATGAATTGTATTACTATTGTCTCTTTCTCGATTCTTATCAACAACAAATCTAAGGGCTCAATTTGTCCAACCAAAGGTCTCGGATAG

Coding sequence (CDS)

ATGGAAATTAATGCTGAAGCAGAACTCGTATTGGCGTGGCACACTTTACCTTGTGCATCGTTATTGTTAATTAAGAAAAATTATAGGAACGTCATGAATGTCTCCATTTATTTGAAGAAATGTCGTAAAGGGAAAGTGAGATATGCGGCCTTAAAATTAGATATGAGCAAAGCATATAATAGAGTAGAATGGTCTTTCATATGTCATGTTACGAGTAAGTTGGAATTTCGAGAAGAGTGGATTGAGCTGGTAATGAATTGTATTACTATTGTCTCTTTCTCGATTCTTATCAACAACAAATCTAAGGGCTCAATTTGTCCAACCAAAGGTCTCGGATAG

Protein sequence

MEINAEAELVLAWHTLPCASLLLIKKNYRNVMNVSIYLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIELVMNCITIVSFSILINNKSKGSICPTKGLG
Homology
BLAST of Lcy12g003330 vs. ExPASy TrEMBL
Match: A0A2P6SDG4 (Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0340391 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.8e-15
Identity = 45/89 (50.56%), Postives = 60/89 (67.42%), Query Frame = 0

Query: 23  LIKKNYRNVMNVSIYLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIE 82
           LI  N      VS  LK+ R GKV Y ALKLDMSKAY+RVEW F+  V  K+ F EEWI 
Sbjct: 564 LISDNSLMAFEVSHCLKRRRSGKVGYCALKLDMSKAYDRVEWVFLEAVMRKMGFGEEWIR 623

Query: 83  LVMNCITIVSFSILINNKSKGSICPTKGL 112
            +M+C++ VS+S +IN + +G + P++GL
Sbjct: 624 WIMDCVSTVSYSFIINGEPRGRVIPSRGL 652

BLAST of Lcy12g003330 vs. ExPASy TrEMBL
Match: A0A2N9GM07 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS28341 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 4.8e-15
Identity = 43/75 (57.33%), Postives = 54/75 (72.00%), Query Frame = 0

Query: 37  YLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIELVMNCITIVSFSIL 96
           Y ++ R GK  Y ALKLDMSKAY+RVEW FI  V  KL F E+WI L+MNCIT V +S+ 
Sbjct: 320 YFQQKRSGKDTYMALKLDMSKAYDRVEWVFIEQVMKKLGFCEKWISLIMNCITTVQYSVF 379

Query: 97  INNKSKGSICPTKGL 112
           +N  + G+I PT+GL
Sbjct: 380 VNGDTCGNIIPTRGL 394

BLAST of Lcy12g003330 vs. ExPASy TrEMBL
Match: A0A5B7BN08 (Reverse transcriptase domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_039618 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 6.3e-15
Identity = 41/74 (55.41%), Postives = 58/74 (78.38%), Query Frame = 0

Query: 38  LKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIELVMNCITIVSFSILI 97
           LK  RKGK+  +ALKLDMSKAY+RVEWSF+  V  ++ F ++W++L+M+C++ VSFS+LI
Sbjct: 150 LKNKRKGKMGQSALKLDMSKAYDRVEWSFLEAVMLRMGFHQKWVDLIMHCVSTVSFSVLI 209

Query: 98  NNKSKGSICPTKGL 112
           N   +G I PT+GL
Sbjct: 210 NGDPRGCIKPTRGL 223

BLAST of Lcy12g003330 vs. ExPASy TrEMBL
Match: A0A2N9INH4 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS54999 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.4e-14
Identity = 44/89 (49.44%), Postives = 56/89 (62.92%), Query Frame = 0

Query: 23  LIKKNYRNVMNVSIYLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIE 82
           LI  N         Y+   R+GKV   ALKLDMSKAY+RVEW F+ HV  K+ F   W+ 
Sbjct: 712 LITDNVLIAFETLHYMHNLRQGKVGSMALKLDMSKAYDRVEWGFLRHVMIKMGFHSRWVS 771

Query: 83  LVMNCITIVSFSILINNKSKGSICPTKGL 112
           L+M CIT VS+S+LIN +  G I P++GL
Sbjct: 772 LMMECITTVSYSLLINGEPTGHITPSRGL 800

BLAST of Lcy12g003330 vs. ExPASy TrEMBL
Match: A0A5B7AER2 (Reverse transcriptase domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_022934 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.8e-14
Identity = 43/89 (48.31%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 23  LIKKNYRNVMNVSIYLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIE 82
           LI  N      +   LK  RKGK+ + ALKLDMSKAY+RVEW F+  +  K+ F  +W+ 
Sbjct: 126 LISVNILVAFEIIHCLKNRRKGKMGHLALKLDMSKAYDRVEWGFLEAIMLKMGFHPQWVS 185

Query: 83  LVMNCITIVSFSILINNKSKGSICPTKGL 112
           LVM+C++ VSFS+L+N   +G I PT+GL
Sbjct: 186 LVMHCVSTVSFSVLVNGDPRGCINPTRGL 214

BLAST of Lcy12g003330 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 95.5 bits (236), Expect = 3.1e-16
Identity = 48/89 (53.93%), Postives = 63/89 (70.79%), Query Frame = 0

Query: 23  LIKKNYRNVMNVSIYLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIE 82
           LI  N      +  YL+  ++GK  +AA+KLDMSKAY+RVEW FI  V  K+ F E+WI+
Sbjct: 385 LITDNVLVAFELMHYLEHKKEGKEGFAAIKLDMSKAYDRVEWGFIKQVMEKMGFHEKWIK 444

Query: 83  LVMNCITIVSFSILINNKSKGSICPTKGL 112
           LVM+CIT VS+SIL+N  + GSI PT+GL
Sbjct: 445 LVMHCITSVSYSILVNGGAYGSITPTRGL 473

BLAST of Lcy12g003330 vs. NCBI nr
Match: XP_023902041.1 (uncharacterized protein LOC112013897 [Quercus suber])

HSP 1 Score: 92.8 bits (229), Expect = 2.0e-15
Identity = 44/75 (58.67%), Postives = 58/75 (77.33%), Query Frame = 0

Query: 37  YLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIELVMNCITIVSFSIL 96
           YL+  ++GK  +AA+KLDMSKAY+RVEW FI  V  K+ F E+WI+LVM+CI  VS+SIL
Sbjct: 215 YLEHKKEGKEGFAAIKLDMSKAYDRVEWGFIKQVMEKMGFHEKWIKLVMHCINSVSYSIL 274

Query: 97  INNKSKGSICPTKGL 112
           +N  + GSI PT+GL
Sbjct: 275 VNGGAYGSITPTRGL 289

BLAST of Lcy12g003330 vs. NCBI nr
Match: PRQ56718.1 (putative RNA-directed DNA polymerase [Rosa chinensis])

HSP 1 Score: 91.3 bits (225), Expect = 5.8e-15
Identity = 45/89 (50.56%), Postives = 60/89 (67.42%), Query Frame = 0

Query: 23  LIKKNYRNVMNVSIYLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIE 82
           LI  N      VS  LK+ R GKV Y ALKLDMSKAY+RVEW F+  V  K+ F EEWI 
Sbjct: 564 LISDNSLMAFEVSHCLKRRRSGKVGYCALKLDMSKAYDRVEWVFLEAVMRKMGFGEEWIR 623

Query: 83  LVMNCITIVSFSILINNKSKGSICPTKGL 112
            +M+C++ VS+S +IN + +G + P++GL
Sbjct: 624 WIMDCVSTVSYSFIINGEPRGRVIPSRGL 652

BLAST of Lcy12g003330 vs. NCBI nr
Match: XP_030939696.1 (uncharacterized protein LOC115964548 [Quercus lobata])

HSP 1 Score: 90.1 bits (222), Expect = 1.3e-14
Identity = 41/76 (53.95%), Postives = 55/76 (72.37%), Query Frame = 0

Query: 37  YLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIELVMNCITIVSFSIL 96
           ++K C   K  + ALKLDMSKAY+RVEW+F+  +  K+ F+  W+ L+M CIT VS+SIL
Sbjct: 456 HMKTCCSRKTGFMALKLDMSKAYDRVEWAFLEKILLKMGFQNSWVSLIMECITTVSYSIL 515

Query: 97  INNKSKGSICPTKGLG 113
           IN + +G I PTKGLG
Sbjct: 516 INGEPQGMIVPTKGLG 531

BLAST of Lcy12g003330 vs. NCBI nr
Match: XP_042972888.1 (uncharacterized protein LOC122304688 [Carya illinoinensis])

HSP 1 Score: 90.1 bits (222), Expect = 1.3e-14
Identity = 45/89 (50.56%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 23  LIKKNYRNVMNVSIYLKKCRKGKVRYAALKLDMSKAYNRVEWSFICHVTSKLEFREEWIE 82
           LI  N      +  YL++ RKGK  Y +LKLDMSKAY+RVEW F+  +  K+ F E W +
Sbjct: 11  LITDNVLVAYELVHYLRQKRKGKKGYMSLKLDMSKAYDRVEWGFLDTIMRKMGFNERWRK 70

Query: 83  LVMNCITIVSFSILINNKSKGSICPTKGL 112
           LVM+C+  VS+SIL+N   KG I PT+GL
Sbjct: 71  LVMSCVQTVSYSILVNGSPKGPIRPTRGL 99

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2P6SDG42.8e-1550.56Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1... [more]
A0A2N9GM074.8e-1557.33Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A5B7BN086.3e-1555.41Reverse transcriptase domain-containing protein OS=Davidia involucrata OX=16924 ... [more]
A0A2N9INH41.4e-1449.44Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A5B7AER21.8e-1448.31Reverse transcriptase domain-containing protein OS=Davidia involucrata OX=16924 ... [more]
Match NameE-valueIdentityDescription
XP_023881891.13.1e-1653.93uncharacterized protein LOC111994244 [Quercus suber][more]
XP_023902041.12.0e-1558.67uncharacterized protein LOC112013897 [Quercus suber][more]
PRQ56718.15.8e-1550.56putative RNA-directed DNA polymerase [Rosa chinensis][more]
XP_030939696.11.3e-1453.95uncharacterized protein LOC115964548 [Quercus lobata][more]
XP_042972888.11.3e-1450.56uncharacterized protein LOC122304688 [Carya illinoinensis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 33..111
e-value: 1.6E-7
score: 31.1
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 30..99
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 30..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g003330.1Lcy12g003330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090304 nucleic acid metabolic process
molecular_function GO:0016740 transferase activity