Lcy12g003210 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g003210
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationChr12: 9564865 .. 9565783 (+)
RNA-Seq ExpressionLcy12g003210
SyntenyLcy12g003210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTCCCCTTCCATGGAAGCATCGCGTATTGCTTCGACTCAGTACTGTAGTCGTCGGCGCTTGTTGCCGATCCGATTTCACCCTCAACCGTTGGCTCGCCGGAATCCTCGACCGCAAAATTCCCCCCTCTTCCAAACCTCTCCACGGCGATTCCCTCACCCTGCCGGTCATTTTCTACTATCACGGCGGAGGCTTCGCCTTCTCTTACGCCGATTCAGCTCTTTCCCACATAACCGCTCATCGATTCGCCAAACAACTACGCGCCGTTATAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTTCCCCTGCCAATACGACGATGGATTCGACGCCTTGAAATTCATCGACGGAATCGGTGACGGAGTCTTGCCGGCGAGGGCGGATCTGGGCCGTTGCTTCATTCTGGGGGAAAGCGCCGGGGGGAATCTGGGGCACCACGTGGCGGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTTGGACTCATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGGCGCCGTTGAGTCTCGGAATTTCGGATTGGTTTTGGAAGGCGTTTCTGCCGGAGGGGGAAGATCGGAACCATTGGGCGGTGAATGTGTTTGGGCCAAATGGAAGGGATGTTTCGAGATTGGAGAAGTTTCCGGCGACGGTGATTTTCGCCGGAGGATTGGATCTGCTGGTTGATCGGCAGAGACTGTATTATGAGGGGCTGAAGAGAATGGGAAAGGATGTAAAGCTGGTGGAGTTTCCCAGCGCTGTTCATGGATTCTTTATCTTCAAGGATTTGCCTGAATATTCTTCCATGATTGAGGAGATGATGGATTTCATTGCCAAGCCAATAGTCTCGCACCCTCTGATGTAA

mRNA sequence

ATGTCGTCCCCTTCCATGGAAGCATCGCTCGTCGGCGCTTGTTGCCGATCCGATTTCACCCTCAACCGTTGGCTCGCCGGAATCCTCGACCGCAAAATTCCCCCCTCTTCCAAACCTCTCCACGGCGATTCCCTCACCCTGCCGGTCATTTTCTACTATCACGGCGGAGGCTTCGCCTTCTCTTACGCCGATTCAGCTCTTTCCCACATAACCGCTCATCGATTCGCCAAACAACTACGCGCCGTTATAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTTCCCCTGCCAATACGACGATGGATTCGACGCCTTGAAATTCATCGACGGAATCGGTGACGGAGTCTTGCCGGCGAGGGCGGATCTGGGCCGTTGCTTCATTCTGGGGGAAAGCGCCGGGGGGAATCTGGGGCACCACGTGGCGGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTTGGACTCATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGGCGCCGTTGAGTCTCGGAATTTCGGATTGGTTTTGGAAGGCGTTTCTGCCGGAGGGGGAAGATCGGAACCATTGGGCGGTGAATGTGTTTGGGCCAAATGGAAGGGATGTTTCGAGATTGGAGAAGTTTCCGGCGACGGTGATTTTCGCCGGAGGATTGGATCTGCTGGTTGATCGGCAGAGACTGTATTATGAGGGGCTGAAGAGAATGGGAAAGGATGTAAAGCTGGTGGAGTTTCCCAGCGCTGTTCATGGATTCTTTATCTTCAAGGATTTGCCTGAATATTCTTCCATGATTGAGGAGATGATGGATTTCATTGCCAAGCCAATAGTCTCGCACCCTCTGATGTAA

Coding sequence (CDS)

ATGTCGTCCCCTTCCATGGAAGCATCGCTCGTCGGCGCTTGTTGCCGATCCGATTTCACCCTCAACCGTTGGCTCGCCGGAATCCTCGACCGCAAAATTCCCCCCTCTTCCAAACCTCTCCACGGCGATTCCCTCACCCTGCCGGTCATTTTCTACTATCACGGCGGAGGCTTCGCCTTCTCTTACGCCGATTCAGCTCTTTCCCACATAACCGCTCATCGATTCGCCAAACAACTACGCGCCGTTATAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTTCCCCTGCCAATACGACGATGGATTCGACGCCTTGAAATTCATCGACGGAATCGGTGACGGAGTCTTGCCGGCGAGGGCGGATCTGGGCCGTTGCTTCATTCTGGGGGAAAGCGCCGGGGGGAATCTGGGGCACCACGTGGCGGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTTGGACTCATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGGCGCCGTTGAGTCTCGGAATTTCGGATTGGTTTTGGAAGGCGTTTCTGCCGGAGGGGGAAGATCGGAACCATTGGGCGGTGAATGTGTTTGGGCCAAATGGAAGGGATGTTTCGAGATTGGAGAAGTTTCCGGCGACGGTGATTTTCGCCGGAGGATTGGATCTGCTGGTTGATCGGCAGAGACTGTATTATGAGGGGCTGAAGAGAATGGGAAAGGATGTAAAGCTGGTGGAGTTTCCCAGCGCTGTTCATGGATTCTTTATCTTCAAGGATTTGCCTGAATATTCTTCCATGATTGAGGAGATGATGGATTTCATTGCCAAGCCAATAGTCTCGCACCCTCTGATGTAA

Protein sequence

MSSPSMEASLVGACCRSDFTLNRWLAGILDRKIPPSSKPLHGDSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIAKPIVSHPLM
Homology
BLAST of Lcy12g003210 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-66
Identity = 134/304 (44.08%), Postives = 180/304 (59.21%), Query Frame = 0

Query: 16  RSDFTLNRWLAGILDRKIPPSSKPL-----------------------HGDSLTLPVIFY 75
           R D T+NR    + D + PP+ KP+                       H     +PV+ +
Sbjct: 33  RPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRDLWFRLYTPHVSGDKIPVVVF 92

Query: 76  YHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFIDG 135
           +HGGGFAF   ++        RFA++L A +ISVNYRLAPE R+P QYDDGFDALK+I+ 
Sbjct: 93  FHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEE 152

Query: 136 IGDGVLPARADLGRCFILGESAGGNLGHHVAV---RASEYEFKRVKVVGLIASQPFFGGE 195
               +LPA ADL RCF  G+SAGGN+ H+VA+   R     F  VK++GLI+ QPFFGGE
Sbjct: 153 NHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGE 212

Query: 196 ERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVI 255
           ERTE+E +L     +S   +DW WKA    G +R+H AVNV GPN  D+S L+ +P T++
Sbjct: 213 ERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDHEAVNVGGPNAVDISGLD-YPETMV 272

Query: 256 FAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIAK 294
              G D L D QR YYE LK  GK   L+E+P+  H F+IF +LPE   +I  + DF+ +
Sbjct: 273 VVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAFYIFPELPEAGQLIMRIKDFVDE 332

BLAST of Lcy12g003210 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.5e-54
Identity = 117/309 (37.86%), Postives = 170/309 (55.02%), Query Frame = 0

Query: 16  RSDFTLNRWLAGILDRKIPPSSKPLHG--------------------------------- 75
           R D T NR LA  LDRK+P ++ P++G                                 
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDL 95

Query: 76  ----DSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPC 135
               D   +PVI ++HGG FA S A+SA+      R      AV++SVNYR APE R+PC
Sbjct: 96  QNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPC 155

Query: 136 QYDDGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVG 195
            YDDG+  LK+++     +   +    R F+ G+S+GGN+ H+VAVRA E    R+ V+G
Sbjct: 156 AYDDGWAVLKWVNS-SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLG 215

Query: 196 LIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDV 255
            I   P FGG ERTESE RL  +  +++   DW+W+AFLPEGEDR H A + FGP  + +
Sbjct: 216 NILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSL 275

Query: 256 SRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSS 288
             L  FP +++   GLDL+ D Q  Y EGLK+ G++VKL+    A  GF++  +   + +
Sbjct: 276 EGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHT 335

BLAST of Lcy12g003210 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.0e-53
Identity = 122/319 (38.24%), Postives = 169/319 (52.98%), Query Frame = 0

Query: 16  RSDFTLNRWLAGILDRKIPPSSKPLHGDS------------------------------- 75
           R+D T  R L   LDR++P +++PL G S                               
Sbjct: 36  RADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGDAEEGAAA 95

Query: 76  --------LT-------LPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYR 135
                   LT        PVI ++HGG F  S A S +      RF K  + V++SVNYR
Sbjct: 96  VTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNYR 155

Query: 136 LAPEFRFPCQYDDGFDALKFIDGIGDGVLPARADL-GRCFILGESAGGNLGHHVAVRASE 195
            APE R+PC YDDG+ ALK++  +    + +  D   R F+ G+S+GGN+ HHVAVRA++
Sbjct: 156 RAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHHVAVRAAD 215

Query: 196 YEFKRVKVVGLIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAV 255
              + VKV G I     FGG ERTESE RL  +  ++L   DW+WKA+LPE  DR+H A 
Sbjct: 216 ---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDADRDHPAC 275

Query: 256 NVFGPNGRDVSRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFF 288
           N FGPNGR +  L  F  ++I   GLDL  DRQ  Y + L+  G  VK+V+  +A  GF+
Sbjct: 276 NPFGPNGRRLGGL-PFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVGFY 335

BLAST of Lcy12g003210 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.2e-51
Identity = 107/263 (40.68%), Postives = 159/263 (60.46%), Query Frame = 0

Query: 30  DRKIPPS----SKPLHGDSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIIS 89
           D++ PPS     KP+ GD   +PVI ++HGG FA S A+SA+      R     + V++S
Sbjct: 87  DQEQPPSILDLEKPVDGD--IVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVS 146

Query: 90  VNYRLAPEFRFPCQYDDGFDALKFIDGIGDGVLPARADLG-RCFILGESAGGNLGHHVAV 149
           VNYR APE  +PC YDDG+ AL +++      L ++ D     F+ G+S+GGN+ H+VA+
Sbjct: 147 VNYRRAPENPYPCAYDDGWIALNWVN--SRSWLKSKKDSKVHIFLAGDSSGGNIAHNVAL 206

Query: 150 RASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRN 209
           RA E     + V+G I   P FGG ERTESE  L  +  +++   DW+WKAFLPEGEDR 
Sbjct: 207 RAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDRE 266

Query: 210 HWAVNVFGPNGRDVSRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAV 269
           H A N F P G+ +  +  FP +++   GLDL+ D Q  Y EGLK+ G++VKL+    A 
Sbjct: 267 HPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKAT 326

Query: 270 HGFFIFKDLPEYSSMIEEMMDFI 288
            GF++  +   + ++++E+  F+
Sbjct: 327 VGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Lcy12g003210 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.4e-49
Identity = 110/311 (35.37%), Postives = 167/311 (53.70%), Query Frame = 0

Query: 16  RSDFTLNRWLAGILDRKIPPSSKPL-------HGDSLT---------------------- 75
           R D + NR LA  LDRK+P +S PL       H DS T                      
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHVDSTTNLLTRIYQPASLLHQTRHGTLE 95

Query: 76  ----------LPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRF 135
                     +PV+ ++HGG F  S A+SA+      R       V++SV+YR +PE R+
Sbjct: 96  LTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHRY 155

Query: 136 PCQYDDGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKV 195
           PC YDDG++AL ++      +   +      ++ G+S+GGN+ H+VAVRA+    + VKV
Sbjct: 156 PCAYDDGWNALNWVKS-RVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---EGVKV 215

Query: 196 VGLIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGR 255
           +G I   P FGG+ERT+SE  L  +  +++   DW+W+A+LPEGEDR+H A N FGP G+
Sbjct: 216 LGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRGQ 275

Query: 256 DVSRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEY 288
            +  +  FP +++   GLDL+ D Q  Y +GLK+ G +V L+    A  GF+   +   +
Sbjct: 276 SLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDHF 335

BLAST of Lcy12g003210 vs. ExPASy TrEMBL
Match: A0A0A0KUP3 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647370 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 5.7e-124
Identity = 217/312 (69.55%), Postives = 248/312 (79.49%), Query Frame = 0

Query: 8   ASLVGACCRSDFTLNRWLAGILDRKIPPSSKPLHG------------------------- 67
           +++  ACCRSDFT+NRW  GILD KIPPS+KP+ G                         
Sbjct: 27  STITDACCRSDFTVNRWFTGILDWKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPVIDG 86

Query: 68  ---DSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQ 127
              D  +LP+IFY+HGGGFAFSYADSALSH +AHRFAKQL AV+ISVNYRLAPEFR+PCQ
Sbjct: 87  EDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPCQ 146

Query: 128 YDDGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGL 187
           YDDGFDALKFID +G+ +LPA+ADL RCFILGESAGGNLGHHVAVRASEY  K+VK+VG 
Sbjct: 147 YDDGFDALKFIDEVGEEILPAKADLTRCFILGESAGGNLGHHVAVRASEYTLKKVKMVGF 206

Query: 188 IASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVS 247
           IASQPFFGGEERTESEIRL NQ PLSL +SDWFWKAFLPEGEDR+H A NVFGP GRDV+
Sbjct: 207 IASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHGAANVFGPKGRDVT 266

Query: 248 RLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSM 292
            + KFPAT++  G LDLL D QR YYEGLKRMGK+VK+VEF +A+HGFF F DLP+YSSM
Sbjct: 267 EVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGKEVKMVEFENAIHGFFAFWDLPQYSSM 326

BLAST of Lcy12g003210 vs. ExPASy TrEMBL
Match: A0A6J1EWT7 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 9.7e-124
Identity = 217/297 (73.06%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 15  CRSDFTLNRWLAGILDRKIPPSSKPLHGDS-----------------------LTLPVIF 74
           CRSDFT+NRWL GILDR+ PPSS P+ G S                        +LPVIF
Sbjct: 33  CRSDFTINRWLTGILDRRTPPSSIPIDGVSSSDLLIDPSRNLWVRIFTPIDAVQSLPVIF 92

Query: 75  YYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFID 134
           YYHGGGFAFSYADS+L H TAH+FAKQLRAV+ISVNYRLAPEFRFPCQYDDGFDALKFID
Sbjct: 93  YYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNYRLAPEFRFPCQYDDGFDALKFID 152

Query: 135 GIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEER 194
            I DG LPA ADLGR FILGESAGGNLGHHVAVRASEY FK++K++G IA+QPFFG EER
Sbjct: 153 EIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASEYAFKKLKLIGFIATQPFFGAEER 212

Query: 195 TESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVIFA 254
           TESEIRL NQAPLSL +SDWFWKAFLP G DR+H A NVFGPNGRD+S +E+FPATV+  
Sbjct: 213 TESEIRLCNQAPLSLRLSDWFWKAFLPVGSDRDHAAANVFGPNGRDISGVERFPATVVLV 272

Query: 255 GGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIA 289
           GG DLL D QR YYEGLKRMGK+VK+VE P+A+HGFF F DLPEYSSMI+E+ DFIA
Sbjct: 273 GGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFFCFPDLPEYSSMIQEVRDFIA 329

BLAST of Lcy12g003210 vs. ExPASy TrEMBL
Match: A0A5D3BN57 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001350 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.7e-123
Identity = 216/307 (70.36%), Postives = 246/307 (80.13%), Query Frame = 0

Query: 10  LVGACCRSDFTLNRWLAGILDRKIPPSSKPLHGDS------------------------- 69
           +  ACCRSDFT+NRWL+G LD KIPPS+KP+ G S                         
Sbjct: 30  ITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIDDED 89

Query: 70  ---LTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYD 129
               +LP+IFY+HGGGFAFSYADSALSH +AHRFAKQL AV+ISVNYRLAPEFR+PCQYD
Sbjct: 90  SNLQSLPLIFYFHGGGFAFSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPCQYD 149

Query: 130 DGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIA 189
           DGFDALKFID +G+ +LPA+ADL RCFILGESAGGNLGHHVAVRASEY  K+V++VG IA
Sbjct: 150 DGFDALKFIDEVGEEILPAKADLSRCFILGESAGGNLGHHVAVRASEYTLKKVRLVGFIA 209

Query: 190 SQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRL 249
           SQPFFGGEERTESEIRLRNQ PL+L +SDWFWKAFLPEGEDR+H A NVFGP GRDV  +
Sbjct: 210 SQPFFGGEERTESEIRLRNQRPLNLRLSDWFWKAFLPEGEDRDHAAANVFGPKGRDVREV 269

Query: 250 EKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIE 289
            KFPAT++  G LDLL D QR YYEGLKRMGK+VK+VEF +A+HGFF F DLP+YSSMI+
Sbjct: 270 MKFPATLVLVGELDLLQDGQRRYYEGLKRMGKEVKMVEFENAIHGFFAFWDLPQYSSMIK 329

BLAST of Lcy12g003210 vs. ExPASy TrEMBL
Match: A0A1S3CAT6 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498754 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.7e-123
Identity = 216/307 (70.36%), Postives = 246/307 (80.13%), Query Frame = 0

Query: 10  LVGACCRSDFTLNRWLAGILDRKIPPSSKPLHGDS------------------------- 69
           +  ACCRSDFT+NRWL+G LD KIPPS+KP+ G S                         
Sbjct: 30  ITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPIIDDED 89

Query: 70  ---LTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYD 129
               +LP+IFY+HGGGFAFSYADSALSH +AHRFAKQL AV+ISVNYRLAPEFR+PCQYD
Sbjct: 90  SNLQSLPLIFYFHGGGFAFSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPCQYD 149

Query: 130 DGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIA 189
           DGFDALKFID +G+ +LPA+ADL RCFILGESAGGNLGHHVAVRASEY  K+V++VG IA
Sbjct: 150 DGFDALKFIDEVGEEILPAKADLSRCFILGESAGGNLGHHVAVRASEYTLKKVRLVGFIA 209

Query: 190 SQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRL 249
           SQPFFGGEERTESEIRLRNQ PL+L +SDWFWKAFLPEGEDR+H A NVFGP GRDV  +
Sbjct: 210 SQPFFGGEERTESEIRLRNQRPLNLRLSDWFWKAFLPEGEDRDHAAANVFGPKGRDVREV 269

Query: 250 EKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIE 289
            KFPAT++  G LDLL D QR YYEGLKRMGK+VK+VEF +A+HGFF F DLP+YSSMI+
Sbjct: 270 MKFPATLVLVGELDLLQDGQRRYYEGLKRMGKEVKMVEFENAIHGFFAFWDLPQYSSMIK 329

BLAST of Lcy12g003210 vs. ExPASy TrEMBL
Match: A0A6J1IB87 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.8e-123
Identity = 215/297 (72.39%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 15  CRSDFTLNRWLAGILDRKIPPSSKPLHG-----------------------DSLTLPVIF 74
           CRSDFT+NRWL GILDR+ PPSSKP+ G                       D  +LPVIF
Sbjct: 33  CRSDFTINRWLTGILDRRTPPSSKPIDGVSSSDLLIDKSRNLWVRIFTPIDDVQSLPVIF 92

Query: 75  YYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFID 134
           YYHGGGFAFSYADS+L H TAH+FAKQLRAV+ISVNYRLAPEFRFPCQYDDGFDALKFID
Sbjct: 93  YYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNYRLAPEFRFPCQYDDGFDALKFID 152

Query: 135 GIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEER 194
            I DG LPA ADLGR FILGESAGGNLGHHVAVRASEY FK++K++G IA+QPF G EER
Sbjct: 153 EIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASEYAFKKLKLIGFIATQPFIGAEER 212

Query: 195 TESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVIFA 254
           TESEIRL NQ PLSL ++DWFWKAFLP G DR+H A NVFGPNGRD+S +EKFPATV+  
Sbjct: 213 TESEIRLCNQPPLSLKLTDWFWKAFLPVGSDRDHAAANVFGPNGRDISGVEKFPATVVLV 272

Query: 255 GGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIA 289
           GG DLL D QR YYEGLKRMGK+VK+VE P+A+HGFF F DLPEYSSMI+ + DFIA
Sbjct: 273 GGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFFCFPDLPEYSSMIQAVRDFIA 329

BLAST of Lcy12g003210 vs. NCBI nr
Match: XP_004141616.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52614.1 hypothetical protein Csa_009173 [Cucumis sativus])

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-123
Identity = 217/312 (69.55%), Postives = 248/312 (79.49%), Query Frame = 0

Query: 8   ASLVGACCRSDFTLNRWLAGILDRKIPPSSKPLHG------------------------- 67
           +++  ACCRSDFT+NRW  GILD KIPPS+KP+ G                         
Sbjct: 27  STITDACCRSDFTVNRWFTGILDWKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPVIDG 86

Query: 68  ---DSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQ 127
              D  +LP+IFY+HGGGFAFSYADSALSH +AHRFAKQL AV+ISVNYRLAPEFR+PCQ
Sbjct: 87  EDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPCQ 146

Query: 128 YDDGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGL 187
           YDDGFDALKFID +G+ +LPA+ADL RCFILGESAGGNLGHHVAVRASEY  K+VK+VG 
Sbjct: 147 YDDGFDALKFIDEVGEEILPAKADLTRCFILGESAGGNLGHHVAVRASEYTLKKVKMVGF 206

Query: 188 IASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVS 247
           IASQPFFGGEERTESEIRL NQ PLSL +SDWFWKAFLPEGEDR+H A NVFGP GRDV+
Sbjct: 207 IASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHGAANVFGPKGRDVT 266

Query: 248 RLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSM 292
            + KFPAT++  G LDLL D QR YYEGLKRMGK+VK+VEF +A+HGFF F DLP+YSSM
Sbjct: 267 EVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGKEVKMVEFENAIHGFFAFWDLPQYSSM 326

BLAST of Lcy12g003210 vs. NCBI nr
Match: KAG6607539.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-123
Identity = 217/297 (73.06%), Postives = 240/297 (80.81%), Query Frame = 0

Query: 15  CRSDFTLNRWLAGILDRKIPPSSKPLHG-----------------------DSLTLPVIF 74
           CRSDFT+NRWL GILDR+ PPSS P+ G                       D  +LPVIF
Sbjct: 33  CRSDFTINRWLTGILDRRTPPSSIPIDGISSSDLLIDPSRNLWVRIFTPIDDVQSLPVIF 92

Query: 75  YYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFID 134
           YYHGGGFAFSYADS+L H TAH+FAKQLRAV+ISVNYRLAPEFRFPCQYDDGFDALKFID
Sbjct: 93  YYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNYRLAPEFRFPCQYDDGFDALKFID 152

Query: 135 GIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEER 194
            I DG LPA ADLGR FILGESAGGNLGHHVAVRASEY FK++K++G IA+QPFFG EER
Sbjct: 153 EIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASEYAFKKLKLIGFIATQPFFGAEER 212

Query: 195 TESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVIFA 254
           TESEIRL NQAPL L +SDWFWKAFLP G DR+H A NVFGPNGRD+S +E+FPATV+  
Sbjct: 213 TESEIRLCNQAPLRLRLSDWFWKAFLPVGSDRDHAAANVFGPNGRDISGVERFPATVVLV 272

Query: 255 GGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIA 289
           GG DLL D QR YYEGLKRMGK+VK+VE P+A+HGFF F DLPEYSSMI+EM DFIA
Sbjct: 273 GGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFFCFPDLPEYSSMIQEMRDFIA 329

BLAST of Lcy12g003210 vs. NCBI nr
Match: XP_022932482.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 453.0 bits (1164), Expect = 2.0e-123
Identity = 217/297 (73.06%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 15  CRSDFTLNRWLAGILDRKIPPSSKPLHGDS-----------------------LTLPVIF 74
           CRSDFT+NRWL GILDR+ PPSS P+ G S                        +LPVIF
Sbjct: 33  CRSDFTINRWLTGILDRRTPPSSIPIDGVSSSDLLIDPSRNLWVRIFTPIDAVQSLPVIF 92

Query: 75  YYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFID 134
           YYHGGGFAFSYADS+L H TAH+FAKQLRAV+ISVNYRLAPEFRFPCQYDDGFDALKFID
Sbjct: 93  YYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNYRLAPEFRFPCQYDDGFDALKFID 152

Query: 135 GIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEER 194
            I DG LPA ADLGR FILGESAGGNLGHHVAVRASEY FK++K++G IA+QPFFG EER
Sbjct: 153 EIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASEYAFKKLKLIGFIATQPFFGAEER 212

Query: 195 TESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVIFA 254
           TESEIRL NQAPLSL +SDWFWKAFLP G DR+H A NVFGPNGRD+S +E+FPATV+  
Sbjct: 213 TESEIRLCNQAPLSLRLSDWFWKAFLPVGSDRDHAAANVFGPNGRDISGVERFPATVVLV 272

Query: 255 GGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIA 289
           GG DLL D QR YYEGLKRMGK+VK+VE P+A+HGFF F DLPEYSSMI+E+ DFIA
Sbjct: 273 GGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFFCFPDLPEYSSMIQEVRDFIA 329

BLAST of Lcy12g003210 vs. NCBI nr
Match: KAG7037181.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 452.2 bits (1162), Expect = 3.4e-123
Identity = 217/297 (73.06%), Postives = 240/297 (80.81%), Query Frame = 0

Query: 15  CRSDFTLNRWLAGILDRKIPPSSKPLHG-----------------------DSLTLPVIF 74
           CRSDFT+NRWL GILDR+ PPSS P+ G                       D  +LPVIF
Sbjct: 33  CRSDFTINRWLTGILDRRTPPSSIPIDGISSSDLLIDPSRNLWVRIFTPIDDVQSLPVIF 92

Query: 75  YYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFID 134
           YYHGGGFAFSYADS+L H TAH+FAKQLRAV+ISVNYRLAPEFRFPCQYDDGFDALKFID
Sbjct: 93  YYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNYRLAPEFRFPCQYDDGFDALKFID 152

Query: 135 GIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEER 194
            I DG LPA ADLGR FILGESAGGNLGHHVAVRASE  FK++K++G IA+QPFFG EER
Sbjct: 153 EIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASECAFKKLKLIGFIATQPFFGAEER 212

Query: 195 TESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVIFA 254
           TESEIRL NQAPLSL +SDWFWKAFLP G DR+H A NVFGPNGRD+S +E+FPATV+  
Sbjct: 213 TESEIRLCNQAPLSLRLSDWFWKAFLPVGSDRDHVAANVFGPNGRDISGVERFPATVVLV 272

Query: 255 GGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIA 289
           GG DLL D QR YYEGLKRMGK+VK+VE P+A+HGFF F DLPEYSSMI+EM DFIA
Sbjct: 273 GGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFFCFPDLPEYSSMIQEMRDFIA 329

BLAST of Lcy12g003210 vs. NCBI nr
Match: KAA0039342.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00525.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 452.2 bits (1162), Expect = 3.4e-123
Identity = 216/307 (70.36%), Postives = 246/307 (80.13%), Query Frame = 0

Query: 10  LVGACCRSDFTLNRWLAGILDRKIPPSSKPLHGDS------------------------- 69
           +  ACCRSDFT+NRWL+G LD KIPPS+KP+ G S                         
Sbjct: 30  ITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIDDED 89

Query: 70  ---LTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYD 129
               +LP+IFY+HGGGFAFSYADSALSH +AHRFAKQL AV+ISVNYRLAPEFR+PCQYD
Sbjct: 90  SNLQSLPLIFYFHGGGFAFSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPCQYD 149

Query: 130 DGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGLIA 189
           DGFDALKFID +G+ +LPA+ADL RCFILGESAGGNLGHHVAVRASEY  K+V++VG IA
Sbjct: 150 DGFDALKFIDEVGEEILPAKADLSRCFILGESAGGNLGHHVAVRASEYTLKKVRLVGFIA 209

Query: 190 SQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRL 249
           SQPFFGGEERTESEIRLRNQ PL+L +SDWFWKAFLPEGEDR+H A NVFGP GRDV  +
Sbjct: 210 SQPFFGGEERTESEIRLRNQRPLNLRLSDWFWKAFLPEGEDRDHAAANVFGPKGRDVREV 269

Query: 250 EKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIE 289
            KFPAT++  G LDLL D QR YYEGLKRMGK+VK+VEF +A+HGFF F DLP+YSSMI+
Sbjct: 270 MKFPATLVLVGELDLLQDGQRRYYEGLKRMGKEVKMVEFENAIHGFFAFWDLPQYSSMIK 329

BLAST of Lcy12g003210 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 254.6 bits (649), Expect = 9.8e-68
Identity = 134/304 (44.08%), Postives = 180/304 (59.21%), Query Frame = 0

Query: 16  RSDFTLNRWLAGILDRKIPPSSKPL-----------------------HGDSLTLPVIFY 75
           R D T+NR    + D + PP+ KP+                       H     +PV+ +
Sbjct: 33  RPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRDLWFRLYTPHVSGDKIPVVVF 92

Query: 76  YHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPCQYDDGFDALKFIDG 135
           +HGGGFAF   ++        RFA++L A +ISVNYRLAPE R+P QYDDGFDALK+I+ 
Sbjct: 93  FHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEE 152

Query: 136 IGDGVLPARADLGRCFILGESAGGNLGHHVAV---RASEYEFKRVKVVGLIASQPFFGGE 195
               +LPA ADL RCF  G+SAGGN+ H+VA+   R     F  VK++GLI+ QPFFGGE
Sbjct: 153 NHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGE 212

Query: 196 ERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDVSRLEKFPATVI 255
           ERTE+E +L     +S   +DW WKA    G +R+H AVNV GPN  D+S L+ +P T++
Sbjct: 213 ERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDHEAVNVGGPNAVDISGLD-YPETMV 272

Query: 256 FAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSSMIEEMMDFIAK 294
              G D L D QR YYE LK  GK   L+E+P+  H F+IF +LPE   +I  + DF+ +
Sbjct: 273 VVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAFYIFPELPEAGQLIMRIKDFVDE 332

BLAST of Lcy12g003210 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 213.4 bits (542), Expect = 2.5e-55
Identity = 117/309 (37.86%), Postives = 170/309 (55.02%), Query Frame = 0

Query: 16  RSDFTLNRWLAGILDRKIPPSSKPLHG--------------------------------- 75
           R D T NR LA  LDRK+P ++ P++G                                 
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDL 95

Query: 76  ----DSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRFPC 135
               D   +PVI ++HGG FA S A+SA+      R      AV++SVNYR APE R+PC
Sbjct: 96  QNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPC 155

Query: 136 QYDDGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKVVG 195
            YDDG+  LK+++     +   +    R F+ G+S+GGN+ H+VAVRA E    R+ V+G
Sbjct: 156 AYDDGWAVLKWVNS-SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLG 215

Query: 196 LIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGRDV 255
            I   P FGG ERTESE RL  +  +++   DW+W+AFLPEGEDR H A + FGP  + +
Sbjct: 216 NILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSL 275

Query: 256 SRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEYSS 288
             L  FP +++   GLDL+ D Q  Y EGLK+ G++VKL+    A  GF++  +   + +
Sbjct: 276 EGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHT 335

BLAST of Lcy12g003210 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 4.4e-52
Identity = 107/263 (40.68%), Postives = 159/263 (60.46%), Query Frame = 0

Query: 30  DRKIPPS----SKPLHGDSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIIS 89
           D++ PPS     KP+ GD   +PVI ++HGG FA S A+SA+      R     + V++S
Sbjct: 87  DQEQPPSILDLEKPVDGD--IVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVS 146

Query: 90  VNYRLAPEFRFPCQYDDGFDALKFIDGIGDGVLPARADLG-RCFILGESAGGNLGHHVAV 149
           VNYR APE  +PC YDDG+ AL +++      L ++ D     F+ G+S+GGN+ H+VA+
Sbjct: 147 VNYRRAPENPYPCAYDDGWIALNWVN--SRSWLKSKKDSKVHIFLAGDSSGGNIAHNVAL 206

Query: 150 RASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRN 209
           RA E     + V+G I   P FGG ERTESE  L  +  +++   DW+WKAFLPEGEDR 
Sbjct: 207 RAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDRE 266

Query: 210 HWAVNVFGPNGRDVSRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAV 269
           H A N F P G+ +  +  FP +++   GLDL+ D Q  Y EGLK+ G++VKL+    A 
Sbjct: 267 HPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKAT 326

Query: 270 HGFFIFKDLPEYSSMIEEMMDFI 288
            GF++  +   + ++++E+  F+
Sbjct: 327 VGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Lcy12g003210 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.4e-50
Identity = 110/311 (35.37%), Postives = 167/311 (53.70%), Query Frame = 0

Query: 16  RSDFTLNRWLAGILDRKIPPSSKPL-------HGDSLT---------------------- 75
           R D + NR LA  LDRK+P +S PL       H DS T                      
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHVDSTTNLLTRIYQPASLLHQTRHGTLE 95

Query: 76  ----------LPVIFYYHGGGFAFSYADSALSHITAHRFAKQLRAVIISVNYRLAPEFRF 135
                     +PV+ ++HGG F  S A+SA+      R       V++SV+YR +PE R+
Sbjct: 96  LTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHRY 155

Query: 136 PCQYDDGFDALKFIDGIGDGVLPARADLGRCFILGESAGGNLGHHVAVRASEYEFKRVKV 195
           PC YDDG++AL ++      +   +      ++ G+S+GGN+ H+VAVRA+    + VKV
Sbjct: 156 PCAYDDGWNALNWVKS-RVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---EGVKV 215

Query: 196 VGLIASQPFFGGEERTESEIRLRNQAPLSLGISDWFWKAFLPEGEDRNHWAVNVFGPNGR 255
           +G I   P FGG+ERT+SE  L  +  +++   DW+W+A+LPEGEDR+H A N FGP G+
Sbjct: 216 LGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRGQ 275

Query: 256 DVSRLEKFPATVIFAGGLDLLVDRQRLYYEGLKRMGKDVKLVEFPSAVHGFFIFKDLPEY 288
            +  +  FP +++   GLDL+ D Q  Y +GLK+ G +V L+    A  GF+   +   +
Sbjct: 276 SLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDHF 335

BLAST of Lcy12g003210 vs. TAIR 10
Match: AT2G45600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.1e-39
Identity = 91/247 (36.84%), Postives = 136/247 (55.06%), Query Frame = 0

Query: 20  TLNRWLAGILDRKIPPSSKPLHGDSLTLPVIFYYHGGGFAFSYADSALSHITAHRFAKQL 79
           T N ++     R IPP SK        LP++ Y+HGGGF    A SA  H +  + A +L
Sbjct: 47  TNNTFIRIFKPRNIPPESK--------LPILVYFHGGGFILYSAASAPFHESCTKMADRL 106

Query: 80  RAVIISVNYRLAPEFRFPCQYDDGFDALKFI---------DGIGDGVLPARADLGRCFIL 139
           + +I+SV YRLAPE R P  Y+D  +A+ ++          G  D  L    D  +C+++
Sbjct: 107 QTIILSVEYRLAPEHRLPAAYEDAVEAILWLRDQARGPINGGDCDTWLKDGVDFSKCYVM 166

Query: 140 GESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQAPLSLGISD 199
           G S+GGN+ ++VA+R  + +   VK+ GLI +Q FFGG E ++SE RL++     L  + 
Sbjct: 167 GSSSGGNIVYNVALRVVDTDLSPVKIQGLIMNQAFFGGVEPSDSESRLKDDKICPLPATH 226

Query: 200 WFWKAFLPEGEDRNHWAVNVFGPNG-RDVSRLEKFPATVIFAGGLDLLVDRQRLYYEGLK 257
             W   LP+G DR+H   N    +G ++  ++ +FP+T+I   G D LVDRQR   E LK
Sbjct: 227 LLWSLCLPDGVDRDHVYSNPIKSSGPQEKDKMGRFPSTLINGYGGDPLVDRQRHVAEMLK 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT101.4e-6644.08Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G63.5e-5437.86Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5453.0e-5338.24Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9MAA76.2e-5140.68Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC13.4e-4935.37Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KUP35.7e-12469.55Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6473... [more]
A0A6J1EWT79.7e-12473.06probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 ... [more]
A0A5D3BN571.7e-12370.36Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CAT61.7e-12370.36probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498754 PE=4 SV=1[more]
A0A6J1IB874.8e-12372.39probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_004141616.11.2e-12369.55probable carboxylesterase 18 [Cucumis sativus] >KGN52614.1 hypothetical protein ... [more]
KAG6607539.11.2e-12373.06putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022932482.12.0e-12373.06probable carboxylesterase 18 [Cucurbita moschata][more]
KAG7037181.13.4e-12373.06putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAA0039342.13.4e-12370.36putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00525.1 putative car... [more]
Match NameE-valueIdentityDescription
AT5G23530.19.8e-6844.08carboxyesterase 18 [more]
AT5G27320.12.5e-5537.86alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.14.4e-5240.68alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.12.4e-5035.37alpha/beta-Hydrolases superfamily protein [more]
AT2G45600.11.1e-3936.84alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 3..291
e-value: 1.4E-70
score: 240.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 46..289
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 50..268
e-value: 6.0E-49
score: 166.8
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 44..290
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 44..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g003210.1Lcy12g003210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity