Lcy12g002610 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g002610 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 8.6e-86
Identity = 214/543 (39.41%), Postives = 313/543 (57.64%), Query Frame = 0

Query: 1   MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
           + EW +A++ +K      I++KLKISY  LE  +Q++FLDIACF + + K   ++IL+S 
Sbjct: 401 LTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESC 460

Query: 61  GFPVVLGLEILEEKSLI-TTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDIN 120
                 GL IL +KSL+  + ++++QMHDLIQ+MG+ IV   F  DP +RSRLWL  ++ 
Sbjct: 461 HIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIV--NFQKDPGERSRLWLAKEVE 520

Query: 121 LALSRDQGTEAIEGIMMDLDEKGESHLKAKSFS--AMTNLRVLKINNVYLSG---ELEYL 180
             +S + GT A+E I +       S+     FS  A+ N++ L++ N+  S     ++YL
Sbjct: 521 EVMSNNTGTMAMEAIWV------SSYSSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDYL 580

Query: 181 SDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFL 240
            + LR      YP +  PS F    L+ L+L  + + HLW   K L  L+ I+LS S+ L
Sbjct: 581 PNNLRCFVCTNYPWESFPSTFELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRL 640

Query: 241 SKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLN 300
           ++TPDF+G+PNLE + L  C  L E+H SLG    +I L L DCK L   P  +++ESL 
Sbjct: 641 TRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFPC-VNVESLE 700

Query: 301 TLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSM----GHLTGLVLLNLKNCKN 360
            L L  C SL+  P+I G M   +++H+ G+ I+ L  S+     H+T L+L N+   KN
Sbjct: 701 YLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNM---KN 760

Query: 361 LVKLPSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTN 420
           LV LPS+I  L SL SL++ GCSKL+S+PE +G++  L   D + T I   P S+  L  
Sbjct: 761 LVALPSSICRLKSLVSLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIRLNK 820

Query: 421 LEILNCQGLSRKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGD 480
           L IL  +G  +  +H  FP           ++GL          SL  LNLS CNL DG 
Sbjct: 821 LIILMFRGF-KDGVHFEFP---------PVAEGL---------HSLEYLNLSYCNLIDGG 880

Query: 481 IPSDLRSLSSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDA 534
           +P ++ SLSSL+ LDLS+N+F  LP SI+ L  L+ L L++C  L  LP+LP  + E+  
Sbjct: 881 LPEEIGSLSSLKKLDLSRNNFEHLPSSIAQLGALQSLDLKDCQRLTQLPELPPELNELHV 911

BLAST of Lcy12g002610 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 3.0e-83
Identity = 207/578 (35.81%), Postives = 297/578 (51.38%), Query Frame = 0

Query: 3   EWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGF 62
           +W   + KL      +I + LK SY  L+  ++ IFLD+ACFFK + +   + IL    F
Sbjct: 410 QWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDF 469

Query: 63  PVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINLAL 122
           P   G+  L +  LIT P++++ MHDLIQ+MG +IV + FP +P K SRLW   D   AL
Sbjct: 470 PAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERAL 529

Query: 123 SRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKI-------------------- 182
           + D+G +++E + +DL +       +  F+ MT LR+LK+                    
Sbjct: 530 TADEGIKSVETMSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEV 589

Query: 183 --------NNVYLSGELEYLSDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLW 242
                   + + L    ++ S +LR+L W GYPL  LP NF+   L+EL L  S I  LW
Sbjct: 590 YDVVMKDASKMQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLW 649

Query: 243 KGPKSLDKLKVINLSDSEFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLD 302
           +G K L++LKVI+LS S  LS+  +FS +PNLERL LSGCV L ++H S+G +K L  L 
Sbjct: 650 QGHKDLERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLS 709

Query: 303 LKDCKQLTSIPFNI-SLESLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPS 362
           L+ C +L ++P +I  LESL +L LS C   + FP+  GNM  L EL L  T+IK L  S
Sbjct: 710 LRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDS 769

Query: 363 MGHLTGLVLLNLKNCKNLVK-----------------------LPSTIGCLTSLKSLNLH 422
           +G L  L  L L NC    K                       LP +IG L SL+ LNL 
Sbjct: 770 IGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLEILNLS 829

Query: 423 GCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLSRKFLHSLFPR 482
            C+K +  PE  GN+  L++LD+  T I + P S+  L +L+ L+    S+      F +
Sbjct: 830 DCAKFEKFPEKGGNMKSLKELDLQNTAIKDLPDSIGDLKSLKYLSLSDCSK------FEK 889

Query: 483 WNNFSRNYSHSQGLKLTNCF------SFG--SSLRILNLSDCNLWDGDIPSDLRSLSSLQ 521
           +     N      L L+N        S G   SL+ L LSDC+ ++   P    ++ SL 
Sbjct: 890 FPEKGGNMKRLLQLILSNTAIKDLPDSIGDLESLKYLYLSDCSKFE-KFPEKGGNMKSLT 949

BLAST of Lcy12g002610 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 7.5e-82
Identity = 228/685 (33.28%), Postives = 341/685 (49.78%), Query Frame = 0

Query: 4    WVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGFP 63
            W   +D+LK     +I E L+ SY  L   ++ +FLDIACFF+ ++      +L S G  
Sbjct: 401  WEAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVD 460

Query: 64   VVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQ-------------KFPNDPQKRS 123
            V   ++ L +K LIT   ++++MHD++Q M ++I  +             +  N  Q   
Sbjct: 461  VSGVVKDLVDKCLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHI 520

Query: 124  RLWLRDDINLALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINN------ 183
            RLW  +DI   L+   GT+ I GI +D  +     L AK+F  M NL+ LKI +      
Sbjct: 521  RLWDSEDICDLLTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRG 580

Query: 184  ------VYLSGELEYLSDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPK 243
                  ++L   L +L ++L +L+WHGYPL+ +P +F+P NL++L+LP S ++ +W   K
Sbjct: 581  CEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEK 640

Query: 244  SLDKLKVINLSDSEFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDC 303
             +  LK ++LS S  L +    +   NLERL L GC  L +L  ++  L+ LI L+L+DC
Sbjct: 641  DVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDC 700

Query: 304  KQLTSIPFNISLESLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLT 363
              L S+P  I  +SL TL+LSGC SLK FP IS N+  LL   LDGT IK L  S+    
Sbjct: 701  TSLRSLPKGIKTQSLQTLILSGCSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFR 760

Query: 364  GLVLLNLKNCKNLVKLPSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCI 423
             L LLNLKNCK L  L S +  L  L+ L L GCS+L+  PE   ++  LE L +  T I
Sbjct: 761  RLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSI 820

Query: 424  NEAPFSLQLLTNLEILNCQGLSRKFLHSLF--PRWNNFSRNYSHSQGLKLTNCFSFGSSL 483
             E P  +  L+N++  +  G S     S+F  P     SR                   L
Sbjct: 821  TEMP-KMMHLSNIKTFSLCGTSSHVSVSMFFMPPTLGCSR-------------------L 880

Query: 484  RILNLSDCNLWDGDIPSDLRSLSSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQ 543
              L LS C+L+   +P ++  LSSLQ L LS N+   LPES + L NL+   L+ C  L+
Sbjct: 881  TDLYLSRCSLY--KLPDNIGGLSSLQSLCLSGNNIENLPESFNQLNNLKWFDLKFCKMLK 940

Query: 544  SLPKLPLSVREVDARDCVSLKEYYNQEKHIPSSE---MGMTFIRCPISTEPAGSYRIDQL 603
            SLP LP +++ +DA +C SL+   N    +   E       F  C    + A +  +   
Sbjct: 941  SLPVLPQNLQYLDAHECESLETLANPLTPLTVGERIHSMFIFSNCYKLNQDAQASLVGHA 1000

Query: 604  GL-SGIHQRTMAQRYLEVLTWQQEDYYFAFPNPSFIACFDDKRYGFSITVHCPPDYVSED 658
             + S +     A+RY       +      +P     + F  +R G S+ +  PP +   D
Sbjct: 1001 RIKSQLMANASAKRYYRGFV-PEPLVGICYPATEIPSWFCHQRLGRSLEIPLPPHWC--D 1057

BLAST of Lcy12g002610 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 6.4e-81
Identity = 223/638 (34.95%), Postives = 322/638 (50.47%), Query Frame = 0

Query: 3    EWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGF 62
            EW + + KL    + EIL  LK SY  L  +E+ IFLD+ACFFK + +    +IL +  F
Sbjct: 415  EWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDF 474

Query: 63   PVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINLAL 122
               +G++ L +K LIT  +++++MHDLIQ+MG +IV +KFP++P K SRLW   D   AL
Sbjct: 475  HAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERAL 534

Query: 123  SRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKI-NNVYLSGELEYL------- 182
            +  +G + +E I +DL +       + +F+ MT LR+LK+ +++ +  E EY+       
Sbjct: 535  TAYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVE 594

Query: 183  ---------------------SDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHL 242
                                 S +LR+L W GYPL  LPSNF+   L+EL L  S I  L
Sbjct: 595  LYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQL 654

Query: 243  WKGPKSLDKLKVINLSDSEFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQL 302
              G K L+ LKVI+LS S  LS+  +FS +PNLERL L GCV L ++H S+G +K L  L
Sbjct: 655  RLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTL 714

Query: 303  DLKDCKQLTSIPFNI-SLESLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHP 362
             LK CK+L ++P +I  LESL  L L+ C   + FP+  GNM  L EL L  T+IK L  
Sbjct: 715  SLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKDLPD 774

Query: 363  SMGHLTGLVLLNLKNCKNLVKLPSTIGCLTSLKSLNLHG--------------------- 422
            S+G L  L  L+L +C    K P   G + SL+ L+L                       
Sbjct: 775  SIGDLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNTAIKDLPDSIRDLESLERLYL 834

Query: 423  --CSKLDSIPESLGNISCLEKLDVTGTCINEAPFS---LQLLTNLEILNC--------QG 482
              CSK +  PE  GN+  L +LD+  T I + P S   L+ L  L++ NC        +G
Sbjct: 835  SYCSKFEKFPEKGGNMKSLMELDLQNTAIKDLPDSIGDLESLKYLDLSNCSKFEKFPEKG 894

Query: 483  LSRKFLHSLFPRWNNFSRNYSHSQG-------LKLTNCFSF------GSSLRILN---LS 539
             + K L  LF   N   ++   S G       L L++C  F      G +++ LN   L+
Sbjct: 895  GNMKSLTELFLE-NTAIKDLPDSIGDLESLVSLNLSDCSKFEKFPEKGGNMKSLNWLYLN 954

BLAST of Lcy12g002610 vs. ExPASy Swiss-Prot
Match: F4JT80 (Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 SV=2)

HSP 1 Score: 302.4 bits (773), Expect = 2.4e-80
Identity = 203/568 (35.74%), Postives = 307/568 (54.05%), Query Frame = 0

Query: 4   WVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGFP 63
           W   ++ L+   D E+ ++LK SY  L++ ++ +FLDIACFF+ +       IL+S    
Sbjct: 402 WKKKLEFLQVNPDKELQKELKSSYKALDDDQKSVFLDIACFFRSEKADFVSSILKSDDID 461

Query: 64  VVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINLALS 123
               +  LEEK L+T  +D+++MHDL+  MG++I  +K      +R RLW   DI   L 
Sbjct: 462 AKDVMRELEEKCLVTISYDRIEMHDLLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDILE 521

Query: 124 RDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGEL------------ 183
            + GTE + GI +++ E     L   +F+ ++ L+ LK ++ + S               
Sbjct: 522 HNTGTECVRGIFLNMSEVRRIKLFPAAFTMLSKLKFLKFHSSHCSQWCDNDHIFQCSKVP 581

Query: 184 EYLSDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDS 243
           ++  D+L +L+W GYP  CLPS+F+P  L++L L  S I  LW+  K+ + L+ ++L  S
Sbjct: 582 DHFPDELVYLHWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQS 641

Query: 244 EFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLE 303
           + L      S   NLERL L GC  L +L  S+  +  LI L+L+DC  L S+P    ++
Sbjct: 642 KDLLNLSGLSRAKNLERLDLEGCTSL-DLLGSVKQMNELIYLNLRDCTSLESLPKGFKIK 701

Query: 304 SLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNL 363
           SL TL+LSGC  LK F  IS ++     LHL+GT+I+ +   +  L  L+LLNLKNC+ L
Sbjct: 702 SLKTLILSGCLKLKDFHIISESIE---SLHLEGTAIERVVEHIESLHSLILLNLKNCEKL 761

Query: 364 VKLPSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNL 423
             LP+ +  L SL+ L L GCS L+S+P     + CLE L + GT I + P  +  L+NL
Sbjct: 762 KYLPNDLYKLKSLQELVLSGCSALESLPPIKEKMECLEILLMDGTSIKQTP-EMSCLSNL 821

Query: 424 EILN-CQGLSRKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGD 483
           +I + C+ +                     S GL +   FS  S L  L L++CN+    
Sbjct: 822 KICSFCRPV------------------IDDSTGLVVLP-FSGNSFLSDLYLTNCNI--DK 881

Query: 484 IPSDLRSLSSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDA 543
           +P    SL SL+ L LS+N+   LPESI  L +L  L L+ C  L+SLP LP +++ +DA
Sbjct: 882 LPDKFSSLRSLRCLCLSRNNIETLPESIEKLYSLLLLDLKHCCRLKSLPLLPSNLQYLDA 941

Query: 544 RDCVSLKEYYNQEKHIP--SSEMGMTFI 557
             C SL E  ++   IP  +  M  TFI
Sbjct: 942 HGCGSL-ENVSKPLTIPLVTERMHTTFI 942

BLAST of Lcy12g002610 vs. ExPASy TrEMBL
Match: A0A6J1CK08 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012131 PE=4 SV=1)

HSP 1 Score: 2127.8 bits (5512), Expect = 0.0e+00
Identity = 1052/1257 (83.69%), Postives = 1133/1257 (90.14%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            ME W NAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQA+EILQSF
Sbjct: 409  MEAWENAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAVEILQSF 468

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GFP VLGLEILEEKSLIT PHDK+QMHDLIQEMGQ+IV QKFPNDP+KRSRLWLR+DINL
Sbjct: 469  GFPAVLGLEILEEKSLITAPHDKIQMHDLIQEMGQEIVRQKFPNDPEKRSRLWLREDINL 528

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMD  EKGES L  KSFSAMTNLRVLK+NNVYL+GELEYLSDQLRF
Sbjct: 529  ALSRDQGTEAIEGIMMDSSEKGESQLNPKSFSAMTNLRVLKVNNVYLNGELEYLSDQLRF 588

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYPLKCLPSNF+P +LLELELP SCI+HLWKG KSLDKLKVINLSDS+FLSKTPD 
Sbjct: 589  LNWHGYPLKCLPSNFHPKSLLELELPCSCIEHLWKGSKSLDKLKVINLSDSQFLSKTPDL 648

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            SGVPNLERL+LSGCVRL ELHQSLGTLKHLIQLDLKDCKQLT+IPFN+SLESLN LVLSG
Sbjct: 649  SGVPNLERLILSGCVRLLELHQSLGTLKHLIQLDLKDCKQLTTIPFNLSLESLNILVLSG 708

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPK+S NMNHL ELHLD TSI++LHPS+GHLTGLVLLNLKNCK LV+LP+TIGC
Sbjct: 709  CSSLKNFPKVSANMNHLSELHLDRTSIRILHPSIGHLTGLVLLNLKNCKYLVQLPTTIGC 768

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK L+L GCSKLD IPESLGNIS LEKLD+TGTCIN+APFSLQLLT+LEILNCQGLS
Sbjct: 769  LTSLKILSLRGCSKLDRIPESLGNISSLEKLDLTGTCINQAPFSLQLLTSLEILNCQGLS 828

Query: 421  RKFLHSLFPRWNNFSRNYSH-SQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSD-LRSL 480
            R FLHSLFP    FSRNYS  SQGLKLTNCFSFGS LR+LNLSDCNLWDGDIP+D LR L
Sbjct: 829  RNFLHSLFP-CLGFSRNYSQSSQGLKLTNCFSFGSCLRVLNLSDCNLWDGDIPNDLLRGL 888

Query: 481  SSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKE 540
             SL+IL LSQNHF+ILPESIS L NLRDLFLEEC +LQSLPKLPLSVR+V+ARDCVSLKE
Sbjct: 889  CSLEILHLSQNHFTILPESISQLTNLRDLFLEECGNLQSLPKLPLSVRDVEARDCVSLKE 948

Query: 541  YYNQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDY 600
            YYNQEKHIPSSEMGMTFIRCPISTEPA SY++DQLGLS IH RTMAQRYLEVLTWQQE Y
Sbjct: 949  YYNQEKHIPSSEMGMTFIRCPISTEPAESYKVDQLGLSAIHLRTMAQRYLEVLTWQQEKY 1008

Query: 601  YFAFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRE--TNNSKV 660
            YF  P P+FIACFDDKRYGFSIT HC PDY SE+N RIGIALGAAF+VQK +   NNSK+
Sbjct: 1009 YFVIPYPNFIACFDDKRYGFSITAHCSPDYTSEENPRIGIALGAAFEVQKHQNNNNNSKL 1068

Query: 661  SCDFIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILT 720
            SCDFIIRMETDECPLKSALV DGN DELD PHGL+VFYIPM +IS WLNQCCCIDVSI+T
Sbjct: 1069 SCDFIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWLNQCCCIDVSIIT 1128

Query: 721  DNPLVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLD 780
            DNPLVKVKWCGASILYEQNAGKFIG IIK  FGSPGKY+TSI+DHILNRQ RVDVSSLLD
Sbjct: 1129 DNPLVKVKWCGASILYEQNAGKFIGRIIKSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 1188

Query: 781  GGTRYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRN 840
            GG RYKT WLNALQRTIGSF RLRPS+PPPE IEDCSTS NAS+E QENE+DS IM+KRN
Sbjct: 1189 GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1248

Query: 841  LKAALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACF 900
            LKA LLRTFE+LKLYGEY++FPQKEISRS F LQLKKPKVTIKVPPNLHKDKKWMG A F
Sbjct: 1249 LKAVLLRTFEELKLYGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAFF 1308

Query: 901  VVFAVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRL 960
            VVFAVDEKS   SHSFSYQ+ENDEY MQRESILYLN+ +F+D HQLWLF+EPRAVYPYRL
Sbjct: 1309 VVFAVDEKS-TKSHSFSYQVENDEYTMQRESILYLNKEMFNDYHQLWLFYEPRAVYPYRL 1368

Query: 961  NHWRHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEY 1020
            NHWRHLCVSF+ +NN  LK V CGARLVYKQD+EGFI  IINNVL SCP DLH FYDQ Y
Sbjct: 1369 NHWRHLCVSFL-SNNPDLKAVACGARLVYKQDLEGFIQMIINNVL-SCPPDLHGFYDQVY 1428

Query: 1021 VKSMLKMINSHKYDPKQKEEERRQD-------STPTSNTASSLVHADQNQERSHILQLKE 1080
            V++ML+MI+ HKYDPK+KEE+RRQD       +    N  S   ++ QN   +HILQLKE
Sbjct: 1429 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1488

Query: 1081 NIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNSDWIGFAVC 1140
            +IPSFLQKDLKDRFGTTFDF+IPRR IPQLFNQQS KNYT I+LPP LYTNS+WIGFAVC
Sbjct: 1489 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1548

Query: 1141 TLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLHERQFIWLY 1200
            TLFQVNKHPTAILNNLRS SRHELICQFA+++GLIEPFHIHTITED FIWLHERQF+WLY
Sbjct: 1549 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1608

Query: 1201 YSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILMEAIQSS 1247
            YSP+ TYGNIFRHKSHI A+IEADTPDLTVRCCGLQLVY +DVE IDK+LMEAIQSS
Sbjct: 1609 YSPKNTYGNIFRHKSHIWAIIEADTPDLTVRCCGLQLVYNQDVEKIDKMLMEAIQSS 1661

BLAST of Lcy12g002610 vs. ExPASy TrEMBL
Match: A0A6J1CJB7 (uncharacterized protein LOC111012131 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012131 PE=4 SV=1)

HSP 1 Score: 2127.8 bits (5512), Expect = 0.0e+00
Identity = 1052/1257 (83.69%), Postives = 1133/1257 (90.14%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            ME W NAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQA+EILQSF
Sbjct: 199  MEAWENAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAVEILQSF 258

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GFP VLGLEILEEKSLIT PHDK+QMHDLIQEMGQ+IV QKFPNDP+KRSRLWLR+DINL
Sbjct: 259  GFPAVLGLEILEEKSLITAPHDKIQMHDLIQEMGQEIVRQKFPNDPEKRSRLWLREDINL 318

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMD  EKGES L  KSFSAMTNLRVLK+NNVYL+GELEYLSDQLRF
Sbjct: 319  ALSRDQGTEAIEGIMMDSSEKGESQLNPKSFSAMTNLRVLKVNNVYLNGELEYLSDQLRF 378

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYPLKCLPSNF+P +LLELELP SCI+HLWKG KSLDKLKVINLSDS+FLSKTPD 
Sbjct: 379  LNWHGYPLKCLPSNFHPKSLLELELPCSCIEHLWKGSKSLDKLKVINLSDSQFLSKTPDL 438

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            SGVPNLERL+LSGCVRL ELHQSLGTLKHLIQLDLKDCKQLT+IPFN+SLESLN LVLSG
Sbjct: 439  SGVPNLERLILSGCVRLLELHQSLGTLKHLIQLDLKDCKQLTTIPFNLSLESLNILVLSG 498

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPK+S NMNHL ELHLD TSI++LHPS+GHLTGLVLLNLKNCK LV+LP+TIGC
Sbjct: 499  CSSLKNFPKVSANMNHLSELHLDRTSIRILHPSIGHLTGLVLLNLKNCKYLVQLPTTIGC 558

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK L+L GCSKLD IPESLGNIS LEKLD+TGTCIN+APFSLQLLT+LEILNCQGLS
Sbjct: 559  LTSLKILSLRGCSKLDRIPESLGNISSLEKLDLTGTCINQAPFSLQLLTSLEILNCQGLS 618

Query: 421  RKFLHSLFPRWNNFSRNYSH-SQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSD-LRSL 480
            R FLHSLFP    FSRNYS  SQGLKLTNCFSFGS LR+LNLSDCNLWDGDIP+D LR L
Sbjct: 619  RNFLHSLFP-CLGFSRNYSQSSQGLKLTNCFSFGSCLRVLNLSDCNLWDGDIPNDLLRGL 678

Query: 481  SSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKE 540
             SL+IL LSQNHF+ILPESIS L NLRDLFLEEC +LQSLPKLPLSVR+V+ARDCVSLKE
Sbjct: 679  CSLEILHLSQNHFTILPESISQLTNLRDLFLEECGNLQSLPKLPLSVRDVEARDCVSLKE 738

Query: 541  YYNQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDY 600
            YYNQEKHIPSSEMGMTFIRCPISTEPA SY++DQLGLS IH RTMAQRYLEVLTWQQE Y
Sbjct: 739  YYNQEKHIPSSEMGMTFIRCPISTEPAESYKVDQLGLSAIHLRTMAQRYLEVLTWQQEKY 798

Query: 601  YFAFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRE--TNNSKV 660
            YF  P P+FIACFDDKRYGFSIT HC PDY SE+N RIGIALGAAF+VQK +   NNSK+
Sbjct: 799  YFVIPYPNFIACFDDKRYGFSITAHCSPDYTSEENPRIGIALGAAFEVQKHQNNNNNSKL 858

Query: 661  SCDFIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILT 720
            SCDFIIRMETDECPLKSALV DGN DELD PHGL+VFYIPM +IS WLNQCCCIDVSI+T
Sbjct: 859  SCDFIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWLNQCCCIDVSIIT 918

Query: 721  DNPLVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLD 780
            DNPLVKVKWCGASILYEQNAGKFIG IIK  FGSPGKY+TSI+DHILNRQ RVDVSSLLD
Sbjct: 919  DNPLVKVKWCGASILYEQNAGKFIGRIIKSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 978

Query: 781  GGTRYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRN 840
            GG RYKT WLNALQRTIGSF RLRPS+PPPE IEDCSTS NAS+E QENE+DS IM+KRN
Sbjct: 979  GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1038

Query: 841  LKAALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACF 900
            LKA LLRTFE+LKLYGEY++FPQKEISRS F LQLKKPKVTIKVPPNLHKDKKWMG A F
Sbjct: 1039 LKAVLLRTFEELKLYGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAFF 1098

Query: 901  VVFAVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRL 960
            VVFAVDEKS   SHSFSYQ+ENDEY MQRESILYLN+ +F+D HQLWLF+EPRAVYPYRL
Sbjct: 1099 VVFAVDEKS-TKSHSFSYQVENDEYTMQRESILYLNKEMFNDYHQLWLFYEPRAVYPYRL 1158

Query: 961  NHWRHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEY 1020
            NHWRHLCVSF+ +NN  LK V CGARLVYKQD+EGFI  IINNVL SCP DLH FYDQ Y
Sbjct: 1159 NHWRHLCVSFL-SNNPDLKAVACGARLVYKQDLEGFIQMIINNVL-SCPPDLHGFYDQVY 1218

Query: 1021 VKSMLKMINSHKYDPKQKEEERRQD-------STPTSNTASSLVHADQNQERSHILQLKE 1080
            V++ML+MI+ HKYDPK+KEE+RRQD       +    N  S   ++ QN   +HILQLKE
Sbjct: 1219 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1278

Query: 1081 NIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNSDWIGFAVC 1140
            +IPSFLQKDLKDRFGTTFDF+IPRR IPQLFNQQS KNYT I+LPP LYTNS+WIGFAVC
Sbjct: 1279 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1338

Query: 1141 TLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLHERQFIWLY 1200
            TLFQVNKHPTAILNNLRS SRHELICQFA+++GLIEPFHIHTITED FIWLHERQF+WLY
Sbjct: 1339 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1398

Query: 1201 YSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILMEAIQSS 1247
            YSP+ TYGNIFRHKSHI A+IEADTPDLTVRCCGLQLVY +DVE IDK+LMEAIQSS
Sbjct: 1399 YSPKNTYGNIFRHKSHIWAIIEADTPDLTVRCCGLQLVYNQDVEKIDKMLMEAIQSS 1451

BLAST of Lcy12g002610 vs. ExPASy TrEMBL
Match: A0A6J1EC12 (TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432680 PE=4 SV=1)

HSP 1 Score: 2069.3 bits (5360), Expect = 0.0e+00
Identity = 1013/1264 (80.14%), Postives = 1122/1264 (88.77%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            M++W NAV KLK+VRDMEILEKLKISYYMLE+SEQKIFLDIACFFKRKSKRQAIEILQSF
Sbjct: 422  MKDWENAVAKLKDVRDMEILEKLKISYYMLEKSEQKIFLDIACFFKRKSKRQAIEILQSF 481

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GF  VLGLE LEEKSLITTPHDK+QMHDLIQEMGQ+IV Q FP++P+KRSRLWLR+D+NL
Sbjct: 482  GFLAVLGLEKLEEKSLITTPHDKIQMHDLIQEMGQEIVRQNFPDEPEKRSRLWLREDVNL 541

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMDLDE+GESHL A SF AMTNLRVLK+NNV+LS +LEYLSDQLRF
Sbjct: 542  ALSRDQGTEAIEGIMMDLDEEGESHLNANSFKAMTNLRVLKLNNVHLSQDLEYLSDQLRF 601

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYP K LPSNF+PTNLLELELPSS I  LWK  K  D LKVINLSDS+FLSKTPDF
Sbjct: 602  LNWHGYPSKFLPSNFHPTNLLELELPSSSIHQLWKDSKRFDTLKVINLSDSKFLSKTPDF 661

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            S VPNLERLVLSGCV L++LHQSLG+LKHLIQLDLKDCKQL++IPFNISLESLN LVLSG
Sbjct: 662  SRVPNLERLVLSGCVSLYQLHQSLGSLKHLIQLDLKDCKQLSNIPFNISLESLNILVLSG 721

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPKISGNMN+LLELHLDGTSIKVLH S+GHLTGLV+LNLKNC NLVKLPSTIGC
Sbjct: 722  CSSLKNFPKISGNMNNLLELHLDGTSIKVLHQSIGHLTGLVILNLKNCTNLVKLPSTIGC 781

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK LNLHGCSK+DSIPESLGNISCLEKLDVT TCI +AP SLQLLTNLEILNC+ LS
Sbjct: 782  LTSLKILNLHGCSKIDSIPESLGNISCLEKLDVTSTCITQAPLSLQLLTNLEILNCRSLS 841

Query: 421  RKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSDLRSLSS 480
            RKF+ SLFP W + SR +S+SQGLKLTNCFSFG SLR+LNLSDCNLWDGD+P DLRSLSS
Sbjct: 842  RKFIQSLFPCW-SLSRKFSNSQGLKLTNCFSFGCSLRVLNLSDCNLWDGDLPMDLRSLSS 901

Query: 481  LQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKEYY 540
            LQIL L+QNHF+ILPESISHL+NLRDLFL EC +L+SLPKLPLSVR+V+ARDCVSL+EYY
Sbjct: 902  LQILHLNQNHFTILPESISHLVNLRDLFLVECSNLRSLPKLPLSVRDVEARDCVSLEEYY 961

Query: 541  NQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDYYF 600
            NQEKHIPSSEMG+TFIRCPISTEPAGSY+ID+LGLS IH RTM+QRY+EVLTWQQE Y+F
Sbjct: 962  NQEKHIPSSEMGITFIRCPISTEPAGSYKIDKLGLSAIHLRTMSQRYIEVLTWQQEKYFF 1021

Query: 601  AFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRETN-NSKVSCD 660
              P P+FIACFDDKRYG SIT HCPPDY+SE+N RIGIALGA F++Q  + N NSK++CD
Sbjct: 1022 LIPYPNFIACFDDKRYGCSITAHCPPDYISEENARIGIALGATFEIQNNQWNENSKITCD 1081

Query: 661  FIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILTDNP 720
            FIIRMETDECPLKSALVFDGN DEL  P GL+VFY+PMRRI GWLNQCCCIDVSI+TDNP
Sbjct: 1082 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRIEGWLNQCCCIDVSIMTDNP 1141

Query: 721  LVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLDGGT 780
             VKVKWCGASI+YEQNAG FIG IIKGLFGSPGKY+TSI+DHILNRQNRVDVSSL+ GG 
Sbjct: 1142 FVKVKWCGASIIYEQNAGSFIGKIIKGLFGSPGKYHTSIVDHILNRQNRVDVSSLVYGGA 1201

Query: 781  RYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRNLKA 840
            RYKTSWLNALQRTIGSF RLR SKPPPEAIED ST M A+ E +E E+D SIM+KRNLKA
Sbjct: 1202 RYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTGMIAAAEAEETESDYSIMLKRNLKA 1261

Query: 841  ALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACFVVF 900
             LLRTFEDLKLYGE+Y+FP+KEISRS F+LQLKKPKVTIK+PPNLHKDKKWMG A FVVF
Sbjct: 1262 MLLRTFEDLKLYGEFYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFFVVF 1321

Query: 901  AVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRLNHW 960
             VDE SP N+HSFSYQ+ENDEY MQRESILYL +GLFDDSHQLW+FFEPRAVYPYRLN W
Sbjct: 1322 GVDENSP-NAHSFSYQVENDEYTMQRESILYLTKGLFDDSHQLWVFFEPRAVYPYRLNQW 1381

Query: 961  RHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEYVKS 1020
            RHLCVSFVCNNNS+LK V+CGARL YK DVEG I+T+INNV+ S PADLHEFYDQ YV+S
Sbjct: 1382 RHLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINNVMGS-PADLHEFYDQVYVES 1441

Query: 1021 MLKMINSHKYDPKQKEEERR------------------QDSTPTSNTASSLVHADQNQER 1080
            M++MI+ HKYDPKQKE E                    QDST TSN            ER
Sbjct: 1442 MIRMIHFHKYDPKQKEAEGEDDLCLEELIEEHNSNGYPQDSTLTSNA----------MER 1501

Query: 1081 SHILQLKENIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNS 1140
            +H+L+LKE IPSFLQKDLKDRFGTTFDF+IPRR+IP+ FNQQS+KN T IQLPP LYTNS
Sbjct: 1502 NHLLELKETIPSFLQKDLKDRFGTTFDFVIPRRNIPEWFNQQSEKNQTAIQLPPSLYTNS 1561

Query: 1141 DWIGFAVCTLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLH 1200
            DW+GFAVC LFQ+NKHPTAILNNLRS+SRHEL+CQFA+++G+I P HIHT+TED+FIWLH
Sbjct: 1562 DWMGFAVCALFQINKHPTAILNNLRSISRHELLCQFAVENGVIHPIHIHTVTEDRFIWLH 1621

Query: 1201 ERQFIWLYYSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILME 1246
            ERQF+W YYSPR+TYGNI RH+SHI A IEADTPD+TVR CGLQLVY +DVE IDKILME
Sbjct: 1622 ERQFLWFYYSPRQTYGNILRHRSHIWATIEADTPDMTVRGCGLQLVYNQDVERIDKILME 1672

BLAST of Lcy12g002610 vs. ExPASy TrEMBL
Match: A0A6J1IBG2 (TMV resistance protein N-like OS=Cucurbita maxima OX=3661 GN=LOC111472024 PE=4 SV=1)

HSP 1 Score: 2063.9 bits (5346), Expect = 0.0e+00
Identity = 1011/1264 (79.98%), Postives = 1121/1264 (88.69%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            M++W NAV KLKEVRD+EILEKLKISYYMLE+SEQKIFLDIACFFKRKSK++AIEILQSF
Sbjct: 406  MKDWENAVAKLKEVRDLEILEKLKISYYMLEKSEQKIFLDIACFFKRKSKKRAIEILQSF 465

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GF  VLGLE LEEKSLIT PHD++QMHDLIQEMGQ+IV Q FPN P+KRSRLWLR+D+NL
Sbjct: 466  GFLAVLGLEKLEEKSLITAPHDQIQMHDLIQEMGQEIVRQNFPNQPEKRSRLWLREDVNL 525

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMDLDE+GESHL A SF AMTNLRVLK+NNVYLS +LEYLSDQLRF
Sbjct: 526  ALSRDQGTEAIEGIMMDLDEEGESHLNANSFKAMTNLRVLKLNNVYLSQDLEYLSDQLRF 585

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYPLK LPSNF+PTNLLELELPSS I  LWK  K  D LKVINLSDS+FLSKTPDF
Sbjct: 586  LNWHGYPLKFLPSNFHPTNLLELELPSSSIHQLWKDSKRFDTLKVINLSDSKFLSKTPDF 645

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            S VPNLERLVLSGCV L++LHQSLG+L+HLIQL+LKDCKQL++IPFNISL+SL  LVLSG
Sbjct: 646  SRVPNLERLVLSGCVSLYQLHQSLGSLRHLIQLELKDCKQLSNIPFNISLQSLKILVLSG 705

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPKISGNMN+LLELHLDGTSIKVLH S+GHLTGLV+LNLKNC NLVKLPSTIGC
Sbjct: 706  CSSLKNFPKISGNMNNLLELHLDGTSIKVLHQSIGHLTGLVILNLKNCTNLVKLPSTIGC 765

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK LNLHGCSK+DSIPESLGNISCLEKLDVT TCI +AP SLQLLTNLEILNCQGLS
Sbjct: 766  LTSLKILNLHGCSKIDSIPESLGNISCLEKLDVTSTCITQAPSSLQLLTNLEILNCQGLS 825

Query: 421  RKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSDLRSLSS 480
            RKF+ SLFP W N SR +S+SQGLKLTNCFSFG SLR+LNLSDCNLWDGD+P+DLRSLSS
Sbjct: 826  RKFIQSLFPCW-NLSRKFSNSQGLKLTNCFSFGCSLRVLNLSDCNLWDGDLPNDLRSLSS 885

Query: 481  LQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKEYY 540
            LQIL L+QNHF+ILPESISHL+NLRDLFL EC +L+SLPKLPLSVR+V+ARDCV L+EYY
Sbjct: 886  LQILHLNQNHFTILPESISHLVNLRDLFLVECLNLRSLPKLPLSVRDVEARDCVLLEEYY 945

Query: 541  NQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDYYF 600
            NQEKHIPSSEMG+TFIRCPISTEPAGSY+IDQLGLS IH RTM+QRY+EVLTWQQE Y+F
Sbjct: 946  NQEKHIPSSEMGITFIRCPISTEPAGSYKIDQLGLSAIHLRTMSQRYIEVLTWQQEKYFF 1005

Query: 601  AFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRETN-NSKVSCD 660
              P P+FIACFDDKRYG SIT HCPPDY+SE+N RIGIALGA F++Q  + N NSK++CD
Sbjct: 1006 VIPYPNFIACFDDKRYGCSITAHCPPDYISEENARIGIALGATFEIQNNQWNENSKITCD 1065

Query: 661  FIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILTDNP 720
            FIIRMETDECPLKSALVFDGN DEL  P GL+VFY+PMRRI GWLNQCCCIDVSI+TDNP
Sbjct: 1066 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRIEGWLNQCCCIDVSIVTDNP 1125

Query: 721  LVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLDGGT 780
             VKVKWCGASI+YEQNAG FIG IIK LFGSPGKY+TSI+DHILNRQNRVDVSSL+DGG 
Sbjct: 1126 FVKVKWCGASIIYEQNAGSFIGKIIKALFGSPGKYHTSIVDHILNRQNRVDVSSLVDGGA 1185

Query: 781  RYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRNLKA 840
            RYKTSWLNALQRTIGSF RLR SKPPPEAIED STSM A+ E +E E+D SIM+KRNLKA
Sbjct: 1186 RYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTSMIAAAEAEETESDYSIMLKRNLKA 1245

Query: 841  ALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACFVVF 900
             LLRTFEDLKLYGEYY+FP+KEISRS F+LQLKKPKVTIK+PPNLHKDKKWMG A FVVF
Sbjct: 1246 MLLRTFEDLKLYGEYYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFFVVF 1305

Query: 901  AVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRLNHW 960
            AVDE SP N+HSFSYQ+ENDEY MQRESILYL +GLFDD HQLW+FFEPRAVYPYRLN W
Sbjct: 1306 AVDENSP-NAHSFSYQVENDEYTMQRESILYLTKGLFDDFHQLWVFFEPRAVYPYRLNQW 1365

Query: 961  RHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEYVKS 1020
            RHLCVSFVCNNNS+LK V+CGARL YK DVEG I+T+IN+V+ S PADLHEFYDQ YV+S
Sbjct: 1366 RHLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINSVMGS-PADLHEFYDQVYVES 1425

Query: 1021 MLKMINSHKYDPKQKEEERR------------------QDSTPTSNTASSLVHADQNQER 1080
            M+KMI+ HKYDPKQKE ER                   QDST TSN            ER
Sbjct: 1426 MIKMIHFHKYDPKQKEFEREDDLCLEELTEEQNSNGYPQDSTLTSNA----------MER 1485

Query: 1081 SHILQLKENIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNS 1140
            +H+L+LKE IPSFLQ DLKDRFGT FDF+IPRR+IP+ FNQ+S+KN T IQLPP LYTNS
Sbjct: 1486 NHLLELKEAIPSFLQMDLKDRFGTIFDFVIPRRNIPEWFNQRSEKNQTGIQLPPSLYTNS 1545

Query: 1141 DWIGFAVCTLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLH 1200
            DW+GFAVC LFQ+NKHPTAILNNLRS+SRHEL+CQF++++G+I P HIHTITED+FIWLH
Sbjct: 1546 DWMGFAVCALFQINKHPTAILNNLRSISRHELLCQFSVENGVIHPIHIHTITEDRFIWLH 1605

Query: 1201 ERQFIWLYYSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILME 1246
            ERQF+WLYYSPR+TYGNI RH+SHI A IEADTPD+TVR CGLQLVY +DVE ID ILME
Sbjct: 1606 ERQFLWLYYSPRQTYGNIIRHRSHIWATIEADTPDMTVRGCGLQLVYNQDVERIDNILME 1656

BLAST of Lcy12g002610 vs. ExPASy TrEMBL
Match: A0A1S3CJJ5 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103501686 PE=4 SV=1)

HSP 1 Score: 1944.5 bits (5036), Expect = 0.0e+00
Identity = 962/1265 (76.05%), Postives = 1079/1265 (85.30%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            ME+W NAV+KLKEV D +ILEKLKI YYMLE+SEQKIFLDIACFFKRKSKRQAIEIL+SF
Sbjct: 421  MEQWENAVEKLKEVCDKKILEKLKIGYYMLEKSEQKIFLDIACFFKRKSKRQAIEILESF 480

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GFP VLGLEILEEKSLIT PHDK+QMHDLIQEMGQ+IV Q FPN+P+KRSRLWLR+DINL
Sbjct: 481  GFPAVLGLEILEEKSLITVPHDKIQMHDLIQEMGQEIVRQNFPNEPEKRSRLWLREDINL 540

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRD+GTEAIEGIMMDLDE+GESHL AKSFSAMTNLRVLK+NNV+L  E+EYLSDQLRF
Sbjct: 541  ALSRDEGTEAIEGIMMDLDEEGESHLNAKSFSAMTNLRVLKVNNVHLCEEIEYLSDQLRF 600

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            +NWHGYPL  LPSNFNPTNLLELELP+S I +LW   KSL+ LKVINLSDS+FLSKTPD 
Sbjct: 601  INWHGYPLTTLPSNFNPTNLLELELPNSSIQNLWTASKSLETLKVINLSDSQFLSKTPDL 660

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            SGVP LERLVLSGCV L +LH SLG LKHL QLDLK CK+LTSIPFNI LESLNT VLSG
Sbjct: 661  SGVPYLERLVLSGCVELHQLHHSLGNLKHLTQLDLKHCKKLTSIPFNICLESLNTFVLSG 720

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C +L  FPKIS NMNHLLELHLD TSIK LH S+GHLTGLVLLNL+NC NL+KLP+TIGC
Sbjct: 721  CSNLTHFPKISANMNHLLELHLDETSIKTLHSSIGHLTGLVLLNLRNCTNLLKLPTTIGC 780

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLKSLNLHGCSKLDS+PESLGNISCLEKLD+T TC+N+AP SLQLLT LEILNCQGLS
Sbjct: 781  LTSLKSLNLHGCSKLDSLPESLGNISCLEKLDITSTCVNQAPMSLQLLTKLEILNCQGLS 840

Query: 421  RKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSDLRSLSS 480
            RKFLHSLFP W NF+R +S+SQGLK+T  F+FG SLR+LNLSDCNLWDGD+P+DL SL+S
Sbjct: 841  RKFLHSLFPTW-NFTRKFSNSQGLKVTIWFNFGCSLRVLNLSDCNLWDGDLPNDLHSLAS 900

Query: 481  LQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKEYY 540
            LQ+LDLSQNHF+ LPESI HL+NLR LFL ECFHL  LPKLPLSVR+VDARDCVSLKEYY
Sbjct: 901  LQVLDLSQNHFTKLPESIRHLVNLRGLFLVECFHLLCLPKLPLSVRDVDARDCVSLKEYY 960

Query: 541  NQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDYYF 600
            NQEK IPSSEMGMT IRCPI+ EP  SY+I Q  LS IH RT  QRYLEVLTWQQE Y+F
Sbjct: 961  NQEKQIPSSEMGMTIIRCPITNEPTQSYKIHQPALSAIHLRTTTQRYLEVLTWQQEQYFF 1020

Query: 601  AFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRETNNS---KVS 660
              P P+FIACFD+KRYGFSIT HCPPDYVSEDN RIGIALGAAF+VQK E +N+   KV 
Sbjct: 1021 VIPYPNFIACFDEKRYGFSITAHCPPDYVSEDNPRIGIALGAAFEVQKHEISNNNSPKVC 1080

Query: 661  CDFIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILTD 720
            CDFI++METDECPLKS LVFDGN DEL    GL VFYIP  RIS WLNQCCCI+VSI+TD
Sbjct: 1081 CDFIVKMETDECPLKSPLVFDGNKDELKSQMGLSVFYIPTNRISRWLNQCCCIEVSIITD 1140

Query: 721  NPLVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLDG 780
            NP VKVKWCGASILYEQNAG FIG IIK LFGSP KY+TSI+DH+LNRQNRVDVS+LLDG
Sbjct: 1141 NPFVKVKWCGASILYEQNAGSFIGKIIKALFGSPDKYHTSIVDHLLNRQNRVDVSTLLDG 1200

Query: 781  GTRYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRNL 840
            G RYKTSW NALQRTIGSF RLRPSK P EA+ DCST MNA+ E +E+E+D SIM+KRNL
Sbjct: 1201 GARYKTSWFNALQRTIGSFPRLRPSKQPREAMLDCST-MNATFEGEESESDYSIMLKRNL 1260

Query: 841  KAALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACFV 900
             A LLRTFE+LKLY EYYIFPQKE+SR  F+ QL++PK+TIK+PPNLHKDKKWMG A FV
Sbjct: 1261 TATLLRTFEELKLYAEYYIFPQKEMSRRFFNFQLEEPKITIKIPPNLHKDKKWMGLAFFV 1320

Query: 901  VFAVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRLN 960
            VF+VDE S   SHSFSYQ++NDEY+M+RES+LYLN+ L   SHQLW+FFEPRAVYPYRLN
Sbjct: 1321 VFSVDENS-QKSHSFSYQVDNDEYRMERESMLYLNKDLLVGSHQLWVFFEPRAVYPYRLN 1380

Query: 961  HWRHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEYV 1020
             WRHL  S VC NNS  K VLCGA LVYKQD+EGF++ I++NVL S PA+LHEFYD+ YV
Sbjct: 1381 QWRHLRFSIVC-NNSDFKAVLCGANLVYKQDLEGFVNIIVSNVL-SSPAELHEFYDRSYV 1440

Query: 1021 KSMLKMINSHKYDPKQKE-EERRQD--------------STPTSNTASSLVHADQNQERS 1080
            +S+L+ ++ HKYDPK+ E ++RRQD              + P  +  SS   +  N ERS
Sbjct: 1441 ESILRNVHCHKYDPKKNENDQRRQDHLRIEKWVEEQDSNAHPQEDEDSS---SSSNMERS 1500

Query: 1081 HILQLKENIPSFLQKDLKDRFGTTFDFIIPRRDI-PQLFNQQSQKNYTTIQLPPGLYTNS 1140
            H   LK++IPSFLQKDLKDR+  TFDF+IPRR+I PQL NQ S +NYT IQLPP  YTN 
Sbjct: 1501 HFSLLKQSIPSFLQKDLKDRYEMTFDFVIPRRNIRPQLINQLSPRNYTRIQLPPNSYTNI 1560

Query: 1141 DWIGFAVCTLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLH 1200
            DW+GFAV T+FQ+NKHPTAILNNL SVSRHELICQF I++GLI P HIH+I EDK IWLH
Sbjct: 1561 DWMGFAVWTVFQINKHPTAILNNLGSVSRHELICQFGIENGLINPLHIHSIIEDKVIWLH 1620

Query: 1201 ERQFIWLYYSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILME 1247
            ERQF+WLYYSPRK YG IFRH+SH+ A+IEADTPDL V CCGLQ+VYKKDV +IDKILME
Sbjct: 1621 ERQFVWLYYSPRKKYGEIFRHRSHVWAIIEADTPDLVVICCGLQVVYKKDVRVIDKILME 1677

BLAST of Lcy12g002610 vs. NCBI nr
Match: XP_022141874.1 (TMV resistance protein N-like isoform X1 [Momordica charantia])

HSP 1 Score: 2127.8 bits (5512), Expect = 0.0e+00
Identity = 1052/1257 (83.69%), Postives = 1133/1257 (90.14%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            ME W NAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQA+EILQSF
Sbjct: 409  MEAWENAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAVEILQSF 468

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GFP VLGLEILEEKSLIT PHDK+QMHDLIQEMGQ+IV QKFPNDP+KRSRLWLR+DINL
Sbjct: 469  GFPAVLGLEILEEKSLITAPHDKIQMHDLIQEMGQEIVRQKFPNDPEKRSRLWLREDINL 528

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMD  EKGES L  KSFSAMTNLRVLK+NNVYL+GELEYLSDQLRF
Sbjct: 529  ALSRDQGTEAIEGIMMDSSEKGESQLNPKSFSAMTNLRVLKVNNVYLNGELEYLSDQLRF 588

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYPLKCLPSNF+P +LLELELP SCI+HLWKG KSLDKLKVINLSDS+FLSKTPD 
Sbjct: 589  LNWHGYPLKCLPSNFHPKSLLELELPCSCIEHLWKGSKSLDKLKVINLSDSQFLSKTPDL 648

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            SGVPNLERL+LSGCVRL ELHQSLGTLKHLIQLDLKDCKQLT+IPFN+SLESLN LVLSG
Sbjct: 649  SGVPNLERLILSGCVRLLELHQSLGTLKHLIQLDLKDCKQLTTIPFNLSLESLNILVLSG 708

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPK+S NMNHL ELHLD TSI++LHPS+GHLTGLVLLNLKNCK LV+LP+TIGC
Sbjct: 709  CSSLKNFPKVSANMNHLSELHLDRTSIRILHPSIGHLTGLVLLNLKNCKYLVQLPTTIGC 768

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK L+L GCSKLD IPESLGNIS LEKLD+TGTCIN+APFSLQLLT+LEILNCQGLS
Sbjct: 769  LTSLKILSLRGCSKLDRIPESLGNISSLEKLDLTGTCINQAPFSLQLLTSLEILNCQGLS 828

Query: 421  RKFLHSLFPRWNNFSRNYSH-SQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSD-LRSL 480
            R FLHSLFP    FSRNYS  SQGLKLTNCFSFGS LR+LNLSDCNLWDGDIP+D LR L
Sbjct: 829  RNFLHSLFP-CLGFSRNYSQSSQGLKLTNCFSFGSCLRVLNLSDCNLWDGDIPNDLLRGL 888

Query: 481  SSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKE 540
             SL+IL LSQNHF+ILPESIS L NLRDLFLEEC +LQSLPKLPLSVR+V+ARDCVSLKE
Sbjct: 889  CSLEILHLSQNHFTILPESISQLTNLRDLFLEECGNLQSLPKLPLSVRDVEARDCVSLKE 948

Query: 541  YYNQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDY 600
            YYNQEKHIPSSEMGMTFIRCPISTEPA SY++DQLGLS IH RTMAQRYLEVLTWQQE Y
Sbjct: 949  YYNQEKHIPSSEMGMTFIRCPISTEPAESYKVDQLGLSAIHLRTMAQRYLEVLTWQQEKY 1008

Query: 601  YFAFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRE--TNNSKV 660
            YF  P P+FIACFDDKRYGFSIT HC PDY SE+N RIGIALGAAF+VQK +   NNSK+
Sbjct: 1009 YFVIPYPNFIACFDDKRYGFSITAHCSPDYTSEENPRIGIALGAAFEVQKHQNNNNNSKL 1068

Query: 661  SCDFIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILT 720
            SCDFIIRMETDECPLKSALV DGN DELD PHGL+VFYIPM +IS WLNQCCCIDVSI+T
Sbjct: 1069 SCDFIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWLNQCCCIDVSIIT 1128

Query: 721  DNPLVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLD 780
            DNPLVKVKWCGASILYEQNAGKFIG IIK  FGSPGKY+TSI+DHILNRQ RVDVSSLLD
Sbjct: 1129 DNPLVKVKWCGASILYEQNAGKFIGRIIKSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 1188

Query: 781  GGTRYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRN 840
            GG RYKT WLNALQRTIGSF RLRPS+PPPE IEDCSTS NAS+E QENE+DS IM+KRN
Sbjct: 1189 GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1248

Query: 841  LKAALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACF 900
            LKA LLRTFE+LKLYGEY++FPQKEISRS F LQLKKPKVTIKVPPNLHKDKKWMG A F
Sbjct: 1249 LKAVLLRTFEELKLYGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAFF 1308

Query: 901  VVFAVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRL 960
            VVFAVDEKS   SHSFSYQ+ENDEY MQRESILYLN+ +F+D HQLWLF+EPRAVYPYRL
Sbjct: 1309 VVFAVDEKS-TKSHSFSYQVENDEYTMQRESILYLNKEMFNDYHQLWLFYEPRAVYPYRL 1368

Query: 961  NHWRHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEY 1020
            NHWRHLCVSF+ +NN  LK V CGARLVYKQD+EGFI  IINNVL SCP DLH FYDQ Y
Sbjct: 1369 NHWRHLCVSFL-SNNPDLKAVACGARLVYKQDLEGFIQMIINNVL-SCPPDLHGFYDQVY 1428

Query: 1021 VKSMLKMINSHKYDPKQKEEERRQD-------STPTSNTASSLVHADQNQERSHILQLKE 1080
            V++ML+MI+ HKYDPK+KEE+RRQD       +    N  S   ++ QN   +HILQLKE
Sbjct: 1429 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1488

Query: 1081 NIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNSDWIGFAVC 1140
            +IPSFLQKDLKDRFGTTFDF+IPRR IPQLFNQQS KNYT I+LPP LYTNS+WIGFAVC
Sbjct: 1489 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1548

Query: 1141 TLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLHERQFIWLY 1200
            TLFQVNKHPTAILNNLRS SRHELICQFA+++GLIEPFHIHTITED FIWLHERQF+WLY
Sbjct: 1549 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1608

Query: 1201 YSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILMEAIQSS 1247
            YSP+ TYGNIFRHKSHI A+IEADTPDLTVRCCGLQLVY +DVE IDK+LMEAIQSS
Sbjct: 1609 YSPKNTYGNIFRHKSHIWAIIEADTPDLTVRCCGLQLVYNQDVEKIDKMLMEAIQSS 1661

BLAST of Lcy12g002610 vs. NCBI nr
Match: XP_022141875.1 (uncharacterized protein LOC111012131 isoform X2 [Momordica charantia])

HSP 1 Score: 2127.8 bits (5512), Expect = 0.0e+00
Identity = 1052/1257 (83.69%), Postives = 1133/1257 (90.14%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            ME W NAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQA+EILQSF
Sbjct: 199  MEAWENAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAVEILQSF 258

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GFP VLGLEILEEKSLIT PHDK+QMHDLIQEMGQ+IV QKFPNDP+KRSRLWLR+DINL
Sbjct: 259  GFPAVLGLEILEEKSLITAPHDKIQMHDLIQEMGQEIVRQKFPNDPEKRSRLWLREDINL 318

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMD  EKGES L  KSFSAMTNLRVLK+NNVYL+GELEYLSDQLRF
Sbjct: 319  ALSRDQGTEAIEGIMMDSSEKGESQLNPKSFSAMTNLRVLKVNNVYLNGELEYLSDQLRF 378

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYPLKCLPSNF+P +LLELELP SCI+HLWKG KSLDKLKVINLSDS+FLSKTPD 
Sbjct: 379  LNWHGYPLKCLPSNFHPKSLLELELPCSCIEHLWKGSKSLDKLKVINLSDSQFLSKTPDL 438

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            SGVPNLERL+LSGCVRL ELHQSLGTLKHLIQLDLKDCKQLT+IPFN+SLESLN LVLSG
Sbjct: 439  SGVPNLERLILSGCVRLLELHQSLGTLKHLIQLDLKDCKQLTTIPFNLSLESLNILVLSG 498

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPK+S NMNHL ELHLD TSI++LHPS+GHLTGLVLLNLKNCK LV+LP+TIGC
Sbjct: 499  CSSLKNFPKVSANMNHLSELHLDRTSIRILHPSIGHLTGLVLLNLKNCKYLVQLPTTIGC 558

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK L+L GCSKLD IPESLGNIS LEKLD+TGTCIN+APFSLQLLT+LEILNCQGLS
Sbjct: 559  LTSLKILSLRGCSKLDRIPESLGNISSLEKLDLTGTCINQAPFSLQLLTSLEILNCQGLS 618

Query: 421  RKFLHSLFPRWNNFSRNYSH-SQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSD-LRSL 480
            R FLHSLFP    FSRNYS  SQGLKLTNCFSFGS LR+LNLSDCNLWDGDIP+D LR L
Sbjct: 619  RNFLHSLFP-CLGFSRNYSQSSQGLKLTNCFSFGSCLRVLNLSDCNLWDGDIPNDLLRGL 678

Query: 481  SSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKE 540
             SL+IL LSQNHF+ILPESIS L NLRDLFLEEC +LQSLPKLPLSVR+V+ARDCVSLKE
Sbjct: 679  CSLEILHLSQNHFTILPESISQLTNLRDLFLEECGNLQSLPKLPLSVRDVEARDCVSLKE 738

Query: 541  YYNQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDY 600
            YYNQEKHIPSSEMGMTFIRCPISTEPA SY++DQLGLS IH RTMAQRYLEVLTWQQE Y
Sbjct: 739  YYNQEKHIPSSEMGMTFIRCPISTEPAESYKVDQLGLSAIHLRTMAQRYLEVLTWQQEKY 798

Query: 601  YFAFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRE--TNNSKV 660
            YF  P P+FIACFDDKRYGFSIT HC PDY SE+N RIGIALGAAF+VQK +   NNSK+
Sbjct: 799  YFVIPYPNFIACFDDKRYGFSITAHCSPDYTSEENPRIGIALGAAFEVQKHQNNNNNSKL 858

Query: 661  SCDFIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILT 720
            SCDFIIRMETDECPLKSALV DGN DELD PHGL+VFYIPM +IS WLNQCCCIDVSI+T
Sbjct: 859  SCDFIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWLNQCCCIDVSIIT 918

Query: 721  DNPLVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLD 780
            DNPLVKVKWCGASILYEQNAGKFIG IIK  FGSPGKY+TSI+DHILNRQ RVDVSSLLD
Sbjct: 919  DNPLVKVKWCGASILYEQNAGKFIGRIIKSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 978

Query: 781  GGTRYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRN 840
            GG RYKT WLNALQRTIGSF RLRPS+PPPE IEDCSTS NAS+E QENE+DS IM+KRN
Sbjct: 979  GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1038

Query: 841  LKAALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACF 900
            LKA LLRTFE+LKLYGEY++FPQKEISRS F LQLKKPKVTIKVPPNLHKDKKWMG A F
Sbjct: 1039 LKAVLLRTFEELKLYGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAFF 1098

Query: 901  VVFAVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRL 960
            VVFAVDEKS   SHSFSYQ+ENDEY MQRESILYLN+ +F+D HQLWLF+EPRAVYPYRL
Sbjct: 1099 VVFAVDEKS-TKSHSFSYQVENDEYTMQRESILYLNKEMFNDYHQLWLFYEPRAVYPYRL 1158

Query: 961  NHWRHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEY 1020
            NHWRHLCVSF+ +NN  LK V CGARLVYKQD+EGFI  IINNVL SCP DLH FYDQ Y
Sbjct: 1159 NHWRHLCVSFL-SNNPDLKAVACGARLVYKQDLEGFIQMIINNVL-SCPPDLHGFYDQVY 1218

Query: 1021 VKSMLKMINSHKYDPKQKEEERRQD-------STPTSNTASSLVHADQNQERSHILQLKE 1080
            V++ML+MI+ HKYDPK+KEE+RRQD       +    N  S   ++ QN   +HILQLKE
Sbjct: 1219 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1278

Query: 1081 NIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNSDWIGFAVC 1140
            +IPSFLQKDLKDRFGTTFDF+IPRR IPQLFNQQS KNYT I+LPP LYTNS+WIGFAVC
Sbjct: 1279 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1338

Query: 1141 TLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLHERQFIWLY 1200
            TLFQVNKHPTAILNNLRS SRHELICQFA+++GLIEPFHIHTITED FIWLHERQF+WLY
Sbjct: 1339 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1398

Query: 1201 YSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILMEAIQSS 1247
            YSP+ TYGNIFRHKSHI A+IEADTPDLTVRCCGLQLVY +DVE IDK+LMEAIQSS
Sbjct: 1399 YSPKNTYGNIFRHKSHIWAIIEADTPDLTVRCCGLQLVYNQDVEKIDKMLMEAIQSS 1451

BLAST of Lcy12g002610 vs. NCBI nr
Match: KAG6592337.1 (Disease resistance protein RUN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2072.7 bits (5369), Expect = 0.0e+00
Identity = 1015/1264 (80.30%), Postives = 1123/1264 (88.84%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            M++W NAV KLK+VRDMEILEKLKISYYMLE+SEQKIFLDIACFFKRKSKRQAIEILQSF
Sbjct: 422  MKDWENAVAKLKDVRDMEILEKLKISYYMLEKSEQKIFLDIACFFKRKSKRQAIEILQSF 481

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GF  VLGLE LEEKSLITTPHDK+QMHDLIQEMGQ+IV Q FP++P+KRSRLWLR+D+NL
Sbjct: 482  GFLAVLGLEKLEEKSLITTPHDKIQMHDLIQEMGQEIVRQNFPDEPEKRSRLWLREDVNL 541

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMDLDE+GESHL A SF AMTNLRVLK+NNV+LS +LEYLSDQLRF
Sbjct: 542  ALSRDQGTEAIEGIMMDLDEEGESHLNANSFKAMTNLRVLKLNNVHLSQDLEYLSDQLRF 601

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYP K LPSNF+PTNLLELELPSS I  LWK  K  D LKVINLSDS+FLSKTPDF
Sbjct: 602  LNWHGYPSKFLPSNFHPTNLLELELPSSSIHQLWKDSKRFDTLKVINLSDSKFLSKTPDF 661

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            S VPNLERLVLSGCV L++LHQSLG+LKHLIQLDLKDCKQL++IPFNISLESLN LVLSG
Sbjct: 662  SRVPNLERLVLSGCVSLYQLHQSLGSLKHLIQLDLKDCKQLSNIPFNISLESLNILVLSG 721

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPKISGNMN+LLELHLDGTSIKVLH S+GHLTGLV+LNLKNC NLVKLPSTIGC
Sbjct: 722  CSSLKNFPKISGNMNNLLELHLDGTSIKVLHQSIGHLTGLVILNLKNCTNLVKLPSTIGC 781

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK LNLHGCSK+DSIPESLGNISCLEKLDVT TCI +AP SLQLLTNLEILNCQ LS
Sbjct: 782  LTSLKILNLHGCSKIDSIPESLGNISCLEKLDVTSTCITQAPLSLQLLTNLEILNCQSLS 841

Query: 421  RKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSDLRSLSS 480
            RKF+ SLFP W + SR +S+SQGLKLTNCFSFG SLR+LNLSDCNLWDGD+P DLRSLSS
Sbjct: 842  RKFIQSLFPCW-SLSRKFSNSQGLKLTNCFSFGCSLRVLNLSDCNLWDGDLPMDLRSLSS 901

Query: 481  LQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKEYY 540
            LQIL L+QNHF+ILPESISHL+NLRDLFL EC +L+SLPKLPLSVR+V+ARDCVSL+EYY
Sbjct: 902  LQILHLNQNHFTILPESISHLVNLRDLFLVECSNLRSLPKLPLSVRDVEARDCVSLEEYY 961

Query: 541  NQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDYYF 600
            NQEKHIPSSEMG+TFIRCPIS EPAGSY+ID+LGLS IH RTM+QRY+EVLTWQQE Y+F
Sbjct: 962  NQEKHIPSSEMGITFIRCPISIEPAGSYKIDKLGLSAIHLRTMSQRYIEVLTWQQEKYFF 1021

Query: 601  AFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRETN-NSKVSCD 660
              P P+FIACFDDKRYG SIT HCPPDY+SE+N RIGIALGA F++Q  + N NSK++CD
Sbjct: 1022 LIPYPNFIACFDDKRYGCSITAHCPPDYISEENARIGIALGATFEIQNNQWNENSKITCD 1081

Query: 661  FIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILTDNP 720
            FIIRMETDECPLKSALVFDGN DEL  P GL+VFY+PMRRI GWLNQCCCIDVSI+TDNP
Sbjct: 1082 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRIEGWLNQCCCIDVSIMTDNP 1141

Query: 721  LVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLDGGT 780
             VKVKWCGASI+YEQNAG FIG IIK LFGSPGKY+TSI+DHILNRQNRVDVSSL+DGG 
Sbjct: 1142 FVKVKWCGASIIYEQNAGSFIGKIIKALFGSPGKYHTSIVDHILNRQNRVDVSSLVDGGA 1201

Query: 781  RYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRNLKA 840
            RYKTSWLNALQRTIGSF RLR SKPPPEAIED ST M A+ E +E E+D SIM+KRNLKA
Sbjct: 1202 RYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTGMIAAAEAEETESDYSIMLKRNLKA 1261

Query: 841  ALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACFVVF 900
             LLRTFEDLKLYGE+Y+FP+KEISRS F+LQLKKPKVTIK+PPNLHKDKKWMG A FVVF
Sbjct: 1262 MLLRTFEDLKLYGEFYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFFVVF 1321

Query: 901  AVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRLNHW 960
            AVDE SP N+HSFSYQ+ENDEY MQRESILYL +GLFDDSHQLW+FFEPRAVYPYRLN W
Sbjct: 1322 AVDENSP-NAHSFSYQVENDEYTMQRESILYLTKGLFDDSHQLWVFFEPRAVYPYRLNQW 1381

Query: 961  RHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEYVKS 1020
            RHLCVSFVCNNNS+LK V+CGARL YK DVEG I+T+INNV+ S PADLHEFYDQ YV+S
Sbjct: 1382 RHLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINNVMGS-PADLHEFYDQVYVES 1441

Query: 1021 MLKMINSHKYDPKQKEEERR------------------QDSTPTSNTASSLVHADQNQER 1080
            M++MI+ HKYDPKQKE E                    QDST TSN            ER
Sbjct: 1442 MIRMIHFHKYDPKQKEAEGEDDLCLEELIEEHNSNGYPQDSTLTSNA----------MER 1501

Query: 1081 SHILQLKENIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNS 1140
            +H+L+LKE IPSFLQKDLKDRFGTTFDF+IPRR+IP+ FNQQS+KN T IQLPP LYTNS
Sbjct: 1502 NHLLELKETIPSFLQKDLKDRFGTTFDFVIPRRNIPEWFNQQSEKNQTAIQLPPSLYTNS 1561

Query: 1141 DWIGFAVCTLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLH 1200
            DW+GFAVC LFQ+NKHPTAILNNLRS+SRHEL+CQFA+++G+I P HIHT+TED+FIWLH
Sbjct: 1562 DWMGFAVCALFQINKHPTAILNNLRSISRHELLCQFAVENGVIHPIHIHTVTEDRFIWLH 1621

Query: 1201 ERQFIWLYYSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILME 1246
            ERQF+WLYYSPR+TYGNI RH+SHI A IEADTPD+TVR CGLQLVY +DVE IDKILME
Sbjct: 1622 ERQFLWLYYSPRQTYGNILRHRSHIWATIEADTPDMTVRGCGLQLVYNQDVERIDKILME 1672

BLAST of Lcy12g002610 vs. NCBI nr
Match: XP_022925371.1 (TMV resistance protein N-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 2069.3 bits (5360), Expect = 0.0e+00
Identity = 1013/1264 (80.14%), Postives = 1122/1264 (88.77%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            M++W NAV KLK+VRDMEILEKLKISYYMLE+SEQKIFLDIACFFKRKSKRQAIEILQSF
Sbjct: 422  MKDWENAVAKLKDVRDMEILEKLKISYYMLEKSEQKIFLDIACFFKRKSKRQAIEILQSF 481

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GF  VLGLE LEEKSLITTPHDK+QMHDLIQEMGQ+IV Q FP++P+KRSRLWLR+D+NL
Sbjct: 482  GFLAVLGLEKLEEKSLITTPHDKIQMHDLIQEMGQEIVRQNFPDEPEKRSRLWLREDVNL 541

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMDLDE+GESHL A SF AMTNLRVLK+NNV+LS +LEYLSDQLRF
Sbjct: 542  ALSRDQGTEAIEGIMMDLDEEGESHLNANSFKAMTNLRVLKLNNVHLSQDLEYLSDQLRF 601

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYP K LPSNF+PTNLLELELPSS I  LWK  K  D LKVINLSDS+FLSKTPDF
Sbjct: 602  LNWHGYPSKFLPSNFHPTNLLELELPSSSIHQLWKDSKRFDTLKVINLSDSKFLSKTPDF 661

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            S VPNLERLVLSGCV L++LHQSLG+LKHLIQLDLKDCKQL++IPFNISLESLN LVLSG
Sbjct: 662  SRVPNLERLVLSGCVSLYQLHQSLGSLKHLIQLDLKDCKQLSNIPFNISLESLNILVLSG 721

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPKISGNMN+LLELHLDGTSIKVLH S+GHLTGLV+LNLKNC NLVKLPSTIGC
Sbjct: 722  CSSLKNFPKISGNMNNLLELHLDGTSIKVLHQSIGHLTGLVILNLKNCTNLVKLPSTIGC 781

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK LNLHGCSK+DSIPESLGNISCLEKLDVT TCI +AP SLQLLTNLEILNC+ LS
Sbjct: 782  LTSLKILNLHGCSKIDSIPESLGNISCLEKLDVTSTCITQAPLSLQLLTNLEILNCRSLS 841

Query: 421  RKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSDLRSLSS 480
            RKF+ SLFP W + SR +S+SQGLKLTNCFSFG SLR+LNLSDCNLWDGD+P DLRSLSS
Sbjct: 842  RKFIQSLFPCW-SLSRKFSNSQGLKLTNCFSFGCSLRVLNLSDCNLWDGDLPMDLRSLSS 901

Query: 481  LQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKEYY 540
            LQIL L+QNHF+ILPESISHL+NLRDLFL EC +L+SLPKLPLSVR+V+ARDCVSL+EYY
Sbjct: 902  LQILHLNQNHFTILPESISHLVNLRDLFLVECSNLRSLPKLPLSVRDVEARDCVSLEEYY 961

Query: 541  NQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDYYF 600
            NQEKHIPSSEMG+TFIRCPISTEPAGSY+ID+LGLS IH RTM+QRY+EVLTWQQE Y+F
Sbjct: 962  NQEKHIPSSEMGITFIRCPISTEPAGSYKIDKLGLSAIHLRTMSQRYIEVLTWQQEKYFF 1021

Query: 601  AFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRETN-NSKVSCD 660
              P P+FIACFDDKRYG SIT HCPPDY+SE+N RIGIALGA F++Q  + N NSK++CD
Sbjct: 1022 LIPYPNFIACFDDKRYGCSITAHCPPDYISEENARIGIALGATFEIQNNQWNENSKITCD 1081

Query: 661  FIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILTDNP 720
            FIIRMETDECPLKSALVFDGN DEL  P GL+VFY+PMRRI GWLNQCCCIDVSI+TDNP
Sbjct: 1082 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRIEGWLNQCCCIDVSIMTDNP 1141

Query: 721  LVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLDGGT 780
             VKVKWCGASI+YEQNAG FIG IIKGLFGSPGKY+TSI+DHILNRQNRVDVSSL+ GG 
Sbjct: 1142 FVKVKWCGASIIYEQNAGSFIGKIIKGLFGSPGKYHTSIVDHILNRQNRVDVSSLVYGGA 1201

Query: 781  RYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRNLKA 840
            RYKTSWLNALQRTIGSF RLR SKPPPEAIED ST M A+ E +E E+D SIM+KRNLKA
Sbjct: 1202 RYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTGMIAAAEAEETESDYSIMLKRNLKA 1261

Query: 841  ALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACFVVF 900
             LLRTFEDLKLYGE+Y+FP+KEISRS F+LQLKKPKVTIK+PPNLHKDKKWMG A FVVF
Sbjct: 1262 MLLRTFEDLKLYGEFYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFFVVF 1321

Query: 901  AVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRLNHW 960
             VDE SP N+HSFSYQ+ENDEY MQRESILYL +GLFDDSHQLW+FFEPRAVYPYRLN W
Sbjct: 1322 GVDENSP-NAHSFSYQVENDEYTMQRESILYLTKGLFDDSHQLWVFFEPRAVYPYRLNQW 1381

Query: 961  RHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEYVKS 1020
            RHLCVSFVCNNNS+LK V+CGARL YK DVEG I+T+INNV+ S PADLHEFYDQ YV+S
Sbjct: 1382 RHLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINNVMGS-PADLHEFYDQVYVES 1441

Query: 1021 MLKMINSHKYDPKQKEEERR------------------QDSTPTSNTASSLVHADQNQER 1080
            M++MI+ HKYDPKQKE E                    QDST TSN            ER
Sbjct: 1442 MIRMIHFHKYDPKQKEAEGEDDLCLEELIEEHNSNGYPQDSTLTSNA----------MER 1501

Query: 1081 SHILQLKENIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNS 1140
            +H+L+LKE IPSFLQKDLKDRFGTTFDF+IPRR+IP+ FNQQS+KN T IQLPP LYTNS
Sbjct: 1502 NHLLELKETIPSFLQKDLKDRFGTTFDFVIPRRNIPEWFNQQSEKNQTAIQLPPSLYTNS 1561

Query: 1141 DWIGFAVCTLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLH 1200
            DW+GFAVC LFQ+NKHPTAILNNLRS+SRHEL+CQFA+++G+I P HIHT+TED+FIWLH
Sbjct: 1562 DWMGFAVCALFQINKHPTAILNNLRSISRHELLCQFAVENGVIHPIHIHTVTEDRFIWLH 1621

Query: 1201 ERQFIWLYYSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILME 1246
            ERQF+W YYSPR+TYGNI RH+SHI A IEADTPD+TVR CGLQLVY +DVE IDKILME
Sbjct: 1622 ERQFLWFYYSPRQTYGNILRHRSHIWATIEADTPDMTVRGCGLQLVYNQDVERIDKILME 1672

BLAST of Lcy12g002610 vs. NCBI nr
Match: XP_022973475.1 (TMV resistance protein N-like [Cucurbita maxima])

HSP 1 Score: 2063.9 bits (5346), Expect = 0.0e+00
Identity = 1011/1264 (79.98%), Postives = 1121/1264 (88.69%), Query Frame = 0

Query: 1    MEEWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSF 60
            M++W NAV KLKEVRD+EILEKLKISYYMLE+SEQKIFLDIACFFKRKSK++AIEILQSF
Sbjct: 406  MKDWENAVAKLKEVRDLEILEKLKISYYMLEKSEQKIFLDIACFFKRKSKKRAIEILQSF 465

Query: 61   GFPVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINL 120
            GF  VLGLE LEEKSLIT PHD++QMHDLIQEMGQ+IV Q FPN P+KRSRLWLR+D+NL
Sbjct: 466  GFLAVLGLEKLEEKSLITAPHDQIQMHDLIQEMGQEIVRQNFPNQPEKRSRLWLREDVNL 525

Query: 121  ALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGELEYLSDQLRF 180
            ALSRDQGTEAIEGIMMDLDE+GESHL A SF AMTNLRVLK+NNVYLS +LEYLSDQLRF
Sbjct: 526  ALSRDQGTEAIEGIMMDLDEEGESHLNANSFKAMTNLRVLKLNNVYLSQDLEYLSDQLRF 585

Query: 181  LNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFLSKTPDF 240
            LNWHGYPLK LPSNF+PTNLLELELPSS I  LWK  K  D LKVINLSDS+FLSKTPDF
Sbjct: 586  LNWHGYPLKFLPSNFHPTNLLELELPSSSIHQLWKDSKRFDTLKVINLSDSKFLSKTPDF 645

Query: 241  SGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLNTLVLSG 300
            S VPNLERLVLSGCV L++LHQSLG+L+HLIQL+LKDCKQL++IPFNISL+SL  LVLSG
Sbjct: 646  SRVPNLERLVLSGCVSLYQLHQSLGSLRHLIQLELKDCKQLSNIPFNISLQSLKILVLSG 705

Query: 301  CWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKLPSTIGC 360
            C SLK+FPKISGNMN+LLELHLDGTSIKVLH S+GHLTGLV+LNLKNC NLVKLPSTIGC
Sbjct: 706  CSSLKNFPKISGNMNNLLELHLDGTSIKVLHQSIGHLTGLVILNLKNCTNLVKLPSTIGC 765

Query: 361  LTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFSLQLLTNLEILNCQGLS 420
            LTSLK LNLHGCSK+DSIPESLGNISCLEKLDVT TCI +AP SLQLLTNLEILNCQGLS
Sbjct: 766  LTSLKILNLHGCSKIDSIPESLGNISCLEKLDVTSTCITQAPSSLQLLTNLEILNCQGLS 825

Query: 421  RKFLHSLFPRWNNFSRNYSHSQGLKLTNCFSFGSSLRILNLSDCNLWDGDIPSDLRSLSS 480
            RKF+ SLFP W N SR +S+SQGLKLTNCFSFG SLR+LNLSDCNLWDGD+P+DLRSLSS
Sbjct: 826  RKFIQSLFPCW-NLSRKFSNSQGLKLTNCFSFGCSLRVLNLSDCNLWDGDLPNDLRSLSS 885

Query: 481  LQILDLSQNHFSILPESISHLINLRDLFLEECFHLQSLPKLPLSVREVDARDCVSLKEYY 540
            LQIL L+QNHF+ILPESISHL+NLRDLFL EC +L+SLPKLPLSVR+V+ARDCV L+EYY
Sbjct: 886  LQILHLNQNHFTILPESISHLVNLRDLFLVECLNLRSLPKLPLSVRDVEARDCVLLEEYY 945

Query: 541  NQEKHIPSSEMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDYYF 600
            NQEKHIPSSEMG+TFIRCPISTEPAGSY+IDQLGLS IH RTM+QRY+EVLTWQQE Y+F
Sbjct: 946  NQEKHIPSSEMGITFIRCPISTEPAGSYKIDQLGLSAIHLRTMSQRYIEVLTWQQEKYFF 1005

Query: 601  AFPNPSFIACFDDKRYGFSITVHCPPDYVSEDNERIGIALGAAFQVQKRETN-NSKVSCD 660
              P P+FIACFDDKRYG SIT HCPPDY+SE+N RIGIALGA F++Q  + N NSK++CD
Sbjct: 1006 VIPYPNFIACFDDKRYGCSITAHCPPDYISEENARIGIALGATFEIQNNQWNENSKITCD 1065

Query: 661  FIIRMETDECPLKSALVFDGNDDELDWPHGLLVFYIPMRRISGWLNQCCCIDVSILTDNP 720
            FIIRMETDECPLKSALVFDGN DEL  P GL+VFY+PMRRI GWLNQCCCIDVSI+TDNP
Sbjct: 1066 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRIEGWLNQCCCIDVSIVTDNP 1125

Query: 721  LVKVKWCGASILYEQNAGKFIGMIIKGLFGSPGKYYTSILDHILNRQNRVDVSSLLDGGT 780
             VKVKWCGASI+YEQNAG FIG IIK LFGSPGKY+TSI+DHILNRQNRVDVSSL+DGG 
Sbjct: 1126 FVKVKWCGASIIYEQNAGSFIGKIIKALFGSPGKYHTSIVDHILNRQNRVDVSSLVDGGA 1185

Query: 781  RYKTSWLNALQRTIGSFQRLRPSKPPPEAIEDCSTSMNASIEVQENENDSSIMIKRNLKA 840
            RYKTSWLNALQRTIGSF RLR SKPPPEAIED STSM A+ E +E E+D SIM+KRNLKA
Sbjct: 1186 RYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTSMIAAAEAEETESDYSIMLKRNLKA 1245

Query: 841  ALLRTFEDLKLYGEYYIFPQKEISRSCFDLQLKKPKVTIKVPPNLHKDKKWMGFACFVVF 900
             LLRTFEDLKLYGEYY+FP+KEISRS F+LQLKKPKVTIK+PPNLHKDKKWMG A FVVF
Sbjct: 1246 MLLRTFEDLKLYGEYYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFFVVF 1305

Query: 901  AVDEKSPNNSHSFSYQLENDEYKMQRESILYLNEGLFDDSHQLWLFFEPRAVYPYRLNHW 960
            AVDE SP N+HSFSYQ+ENDEY MQRESILYL +GLFDD HQLW+FFEPRAVYPYRLN W
Sbjct: 1306 AVDENSP-NAHSFSYQVENDEYTMQRESILYLTKGLFDDFHQLWVFFEPRAVYPYRLNQW 1365

Query: 961  RHLCVSFVCNNNSALKVVLCGARLVYKQDVEGFIDTIINNVLVSCPADLHEFYDQEYVKS 1020
            RHLCVSFVCNNNS+LK V+CGARL YK DVEG I+T+IN+V+ S PADLHEFYDQ YV+S
Sbjct: 1366 RHLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINSVMGS-PADLHEFYDQVYVES 1425

Query: 1021 MLKMINSHKYDPKQKEEERR------------------QDSTPTSNTASSLVHADQNQER 1080
            M+KMI+ HKYDPKQKE ER                   QDST TSN            ER
Sbjct: 1426 MIKMIHFHKYDPKQKEFEREDDLCLEELTEEQNSNGYPQDSTLTSNA----------MER 1485

Query: 1081 SHILQLKENIPSFLQKDLKDRFGTTFDFIIPRRDIPQLFNQQSQKNYTTIQLPPGLYTNS 1140
            +H+L+LKE IPSFLQ DLKDRFGT FDF+IPRR+IP+ FNQ+S+KN T IQLPP LYTNS
Sbjct: 1486 NHLLELKEAIPSFLQMDLKDRFGTIFDFVIPRRNIPEWFNQRSEKNQTGIQLPPSLYTNS 1545

Query: 1141 DWIGFAVCTLFQVNKHPTAILNNLRSVSRHELICQFAIKDGLIEPFHIHTITEDKFIWLH 1200
            DW+GFAVC LFQ+NKHPTAILNNLRS+SRHEL+CQF++++G+I P HIHTITED+FIWLH
Sbjct: 1546 DWMGFAVCALFQINKHPTAILNNLRSISRHELLCQFSVENGVIHPIHIHTITEDRFIWLH 1605

Query: 1201 ERQFIWLYYSPRKTYGNIFRHKSHICALIEADTPDLTVRCCGLQLVYKKDVEMIDKILME 1246
            ERQF+WLYYSPR+TYGNI RH+SHI A IEADTPD+TVR CGLQLVY +DVE ID ILME
Sbjct: 1606 ERQFLWLYYSPRQTYGNIIRHRSHIWATIEADTPDMTVRGCGLQLVYNQDVERIDNILME 1656

BLAST of Lcy12g002610 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 312.0 bits (798), Expect = 2.2e-84
Identity = 245/783 (31.29%), Postives = 367/783 (46.87%), Query Frame = 0

Query: 3    EWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGF 62
            EW + + +LK     +I+E L++SY  L+E E+ IFL I+CF+  K      ++L   G+
Sbjct: 399  EWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGY 458

Query: 63   PVVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINLAL 122
               +G+ IL EKSLI   +  +++HDL+++MG+++V Q+  N+P +R  LW  +DI   L
Sbjct: 459  AAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLL 518

Query: 123  SRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINNVYLSGE--------LEYL 182
            S + GT+ +EGI ++L E  E     ++F  ++NL++L   ++   GE        L YL
Sbjct: 519  SENSGTQLVEGISLNLSEISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYL 578

Query: 183  SDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPKSLDKLKVINLSDSEFL 242
              +LR+L W GYPLK +PS F P  L+EL + +S ++ LW G + L  LK ++LS  ++L
Sbjct: 579  PRKLRYLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYL 638

Query: 243  SKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSIPFNISLESLN 302
             + PD S   NLE L LS C  L E+  S+  LK L    L +C QL  IP  I L+SL 
Sbjct: 639  VEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLE 698

Query: 303  TLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLNLKNCKNLVKL 362
            T+ +SGC SLK FP+IS N      L+L  T I+ L  S+  L+ LV L++ +C+ L  L
Sbjct: 699  TVGMSGCSSLKHFPEISWNTR---RLYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTL 758

Query: 363  PSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCIN--------------- 422
            PS +G L SLKSLNL GC +L+++P++L N++ LE L+V+G C+N               
Sbjct: 759  PSYLGHLVSLKSLNLDGCRRLENLPDTLQNLTSLETLEVSG-CLNVNEFPRVSTSIEVLR 818

Query: 423  -------------------------------EAPFSLQLLTNLEILNCQGLSRKFLHSLF 482
                                             P S+  L +LE L   G S   +   F
Sbjct: 819  ISETSIEEIPARICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCS---VLESF 878

Query: 483  P----------RWNNFSR----------------------------------NYSHSQGL 542
            P          RW +  R                                    +  Q L
Sbjct: 879  PLEICQTMSCLRWFDLDRTSIKELPENIGNLVALEVLQASRTVIRRAPWSIARLTRLQVL 938

Query: 543  KLTNCF--------------SFGSSLRILNLSDCNLWDGDIPSDLRSLSSLQILDLSQNH 602
             + N F              S    LR L+LS+ N+   +IP+ + +L +L  LDLS N+
Sbjct: 939  AIGNSFFTPEGLLHSLCPPLSRFDDLRALSLSNMNM--TEIPNSIGNLWNLLELDLSGNN 998

Query: 603  FSILPESISHLINLRDLFLEECFHLQSLP-KLPLSVREVDARDCVSLKEYYNQEKHIPSS 662
            F  +P SI  L  L  L L  C  LQ+LP +LP  +  +    C SL            S
Sbjct: 999  FEFIPASIKRLTRLNRLNLNNCQRLQALPDELPRGLLYIYIHSCTSL-----------VS 1058

Query: 663  EMGMTFIRCPISTEPAGSYRIDQLGLSGIHQRTMAQRYLEVLTWQQEDYYFAFPNPSFIA 672
              G     C      +  Y++DQ     IH      R L++ + + E  Y  FP      
Sbjct: 1059 ISGCFNQYCLRKLVASNCYKLDQAAQILIH------RNLKLESAKPEHSY--FPGSDIPT 1118

BLAST of Lcy12g002610 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 307.4 bits (786), Expect = 5.3e-83
Identity = 228/685 (33.28%), Postives = 341/685 (49.78%), Query Frame = 0

Query: 4    WVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGFP 63
            W   +D+LK     +I E L+ SY  L   ++ +FLDIACFF+ ++      +L S G  
Sbjct: 401  WEAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVD 460

Query: 64   VVLGLEILEEKSLITTPHDKLQMHDLIQEMGQDIVCQ-------------KFPNDPQKRS 123
            V   ++ L +K LIT   ++++MHD++Q M ++I  +             +  N  Q   
Sbjct: 461  VSGVVKDLVDKCLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHI 520

Query: 124  RLWLRDDINLALSRDQGTEAIEGIMMDLDEKGESHLKAKSFSAMTNLRVLKINN------ 183
            RLW  +DI   L+   GT+ I GI +D  +     L AK+F  M NL+ LKI +      
Sbjct: 521  RLWDSEDICDLLTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRG 580

Query: 184  ------VYLSGELEYLSDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPK 243
                  ++L   L +L ++L +L+WHGYPL+ +P +F+P NL++L+LP S ++ +W   K
Sbjct: 581  CEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEK 640

Query: 244  SLDKLKVINLSDSEFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDC 303
             +  LK ++LS S  L +    +   NLERL L GC  L +L  ++  L+ LI L+L+DC
Sbjct: 641  DVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDC 700

Query: 304  KQLTSIPFNISLESLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLT 363
              L S+P  I  +SL TL+LSGC SLK FP IS N+  LL   LDGT IK L  S+    
Sbjct: 701  TSLRSLPKGIKTQSLQTLILSGCSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFR 760

Query: 364  GLVLLNLKNCKNLVKLPSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCI 423
             L LLNLKNCK L  L S +  L  L+ L L GCS+L+  PE   ++  LE L +  T I
Sbjct: 761  RLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSI 820

Query: 424  NEAPFSLQLLTNLEILNCQGLSRKFLHSLF--PRWNNFSRNYSHSQGLKLTNCFSFGSSL 483
             E P  +  L+N++  +  G S     S+F  P     SR                   L
Sbjct: 821  TEMP-KMMHLSNIKTFSLCGTSSHVSVSMFFMPPTLGCSR-------------------L 880

Query: 484  RILNLSDCNLWDGDIPSDLRSLSSLQILDLSQNHFSILPESISHLINLRDLFLEECFHLQ 543
              L LS C+L+   +P ++  LSSLQ L LS N+   LPES + L NL+   L+ C  L+
Sbjct: 881  TDLYLSRCSLY--KLPDNIGGLSSLQSLCLSGNNIENLPESFNQLNNLKWFDLKFCKMLK 940

Query: 544  SLPKLPLSVREVDARDCVSLKEYYNQEKHIPSSE---MGMTFIRCPISTEPAGSYRIDQL 603
            SLP LP +++ +DA +C SL+   N    +   E       F  C    + A +  +   
Sbjct: 941  SLPVLPQNLQYLDAHECESLETLANPLTPLTVGERIHSMFIFSNCYKLNQDAQASLVGHA 1000

Query: 604  GL-SGIHQRTMAQRYLEVLTWQQEDYYFAFPNPSFIACFDDKRYGFSITVHCPPDYVSED 658
             + S +     A+RY       +      +P     + F  +R G S+ +  PP +   D
Sbjct: 1001 RIKSQLMANASAKRYYRGFV-PEPLVGICYPATEIPSWFCHQRLGRSLEIPLPPHWC--D 1057

BLAST of Lcy12g002610 vs. TAIR 10
Match: AT4G12020.1 (protein kinase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 1.7e-73
Identity = 177/430 (41.16%), Postives = 243/430 (56.51%), Query Frame = 0

Query: 3    EWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGF 62
            EW     ++K    + I    + S   L+++E+ IFLDIACFF R  K     +L   GF
Sbjct: 1024 EWNKLSQEVKTTSPIYIPGIFEKSCCGLDDNERGIFLDIACFFNRIDKDNVAMLLDGCGF 1083

Query: 63   PVVLGLEILEEKSLIT-TPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINLA 122
               +G   L +KSL+T + H+ + M   IQ  G++IV Q+  + P  RSRLW  D I   
Sbjct: 1084 SAHVGFRGLVDKSLLTISQHNLVDMLSFIQATGREIVRQESADRPGDRSRLWNADYIRHV 1143

Query: 123  LSRDQGTEAIEGIMMD-LDEKGESHLKAKSFSAMTNLRVLKI--------NNVYLSGELE 182
               D GT AIEGI +D L+ K +++     F  M NLR+LK+        + V     LE
Sbjct: 1144 FINDTGTSAIEGIFLDMLNLKFDAN--PNVFEKMCNLRLLKLYCSKAEEKHGVSFPQGLE 1203

Query: 183  YLSDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPK--------SLDKLK 242
            YL  +LR L+W  YPL  LP +FNP NL+EL LPSSC   LWKG K        SL+KLK
Sbjct: 1204 YLPSKLRLLHWEYYPLSSLPKSFNPENLVELNLPSSCAKKLWKGKKARFCTTNSSLEKLK 1263

Query: 243  VINLSDSEFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSI 302
             + LS S+ L+K P  S   NLE + L GC  L  L QS+  LK L+ L+LK C +L +I
Sbjct: 1264 KMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLKGCSKLENI 1323

Query: 303  PFNISLESLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLN 362
            P  + LESL  L LSGC  L +FP+IS N+    EL++ GT I+ +  S+ +L  L  L+
Sbjct: 1324 PSMVDLESLEVLNLSGCSKLGNFPEISPNVK---ELYMGGTMIQEIPSSIKNLVLLEKLD 1383

Query: 363  LKNCKNLVKLPSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFS 415
            L+N ++L  LP++I  L  L++LNL GC  L+  P+S   + CL  LD++ T I E P S
Sbjct: 1384 LENSRHLKNLPTSIYKLKHLETLNLSGCISLERFPDSSRRMKCLRFLDLSRTDIKELPSS 1443

BLAST of Lcy12g002610 vs. TAIR 10
Match: AT4G12020.2 (protein kinase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 1.7e-73
Identity = 177/430 (41.16%), Postives = 243/430 (56.51%), Query Frame = 0

Query: 3    EWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGF 62
            EW     ++K    + I    + S   L+++E+ IFLDIACFF R  K     +L   GF
Sbjct: 1024 EWNKLSQEVKTTSPIYIPGIFEKSCCGLDDNERGIFLDIACFFNRIDKDNVAMLLDGCGF 1083

Query: 63   PVVLGLEILEEKSLIT-TPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINLA 122
               +G   L +KSL+T + H+ + M   IQ  G++IV Q+  + P  RSRLW  D I   
Sbjct: 1084 SAHVGFRGLVDKSLLTISQHNLVDMLSFIQATGREIVRQESADRPGDRSRLWNADYIRHV 1143

Query: 123  LSRDQGTEAIEGIMMD-LDEKGESHLKAKSFSAMTNLRVLKI--------NNVYLSGELE 182
               D GT AIEGI +D L+ K +++     F  M NLR+LK+        + V     LE
Sbjct: 1144 FINDTGTSAIEGIFLDMLNLKFDAN--PNVFEKMCNLRLLKLYCSKAEEKHGVSFPQGLE 1203

Query: 183  YLSDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPK--------SLDKLK 242
            YL  +LR L+W  YPL  LP +FNP NL+EL LPSSC   LWKG K        SL+KLK
Sbjct: 1204 YLPSKLRLLHWEYYPLSSLPKSFNPENLVELNLPSSCAKKLWKGKKARFCTTNSSLEKLK 1263

Query: 243  VINLSDSEFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSI 302
             + LS S+ L+K P  S   NLE + L GC  L  L QS+  LK L+ L+LK C +L +I
Sbjct: 1264 KMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLKGCSKLENI 1323

Query: 303  PFNISLESLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLN 362
            P  + LESL  L LSGC  L +FP+IS N+    EL++ GT I+ +  S+ +L  L  L+
Sbjct: 1324 PSMVDLESLEVLNLSGCSKLGNFPEISPNVK---ELYMGGTMIQEIPSSIKNLVLLEKLD 1383

Query: 363  LKNCKNLVKLPSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFS 415
            L+N ++L  LP++I  L  L++LNL GC  L+  P+S   + CL  LD++ T I E P S
Sbjct: 1384 LENSRHLKNLPTSIYKLKHLETLNLSGCISLERFPDSSRRMKCLRFLDLSRTDIKELPSS 1443

BLAST of Lcy12g002610 vs. TAIR 10
Match: AT4G12020.3 (protein kinase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 1.7e-73
Identity = 177/430 (41.16%), Postives = 243/430 (56.51%), Query Frame = 0

Query: 3    EWVNAVDKLKEVRDMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAIEILQSFGF 62
            EW     ++K    + I    + S   L+++E+ IFLDIACFF R  K     +L   GF
Sbjct: 1024 EWNKLSQEVKTTSPIYIPGIFEKSCCGLDDNERGIFLDIACFFNRIDKDNVAMLLDGCGF 1083

Query: 63   PVVLGLEILEEKSLIT-TPHDKLQMHDLIQEMGQDIVCQKFPNDPQKRSRLWLRDDINLA 122
               +G   L +KSL+T + H+ + M   IQ  G++IV Q+  + P  RSRLW  D I   
Sbjct: 1084 SAHVGFRGLVDKSLLTISQHNLVDMLSFIQATGREIVRQESADRPGDRSRLWNADYIRHV 1143

Query: 123  LSRDQGTEAIEGIMMD-LDEKGESHLKAKSFSAMTNLRVLKI--------NNVYLSGELE 182
               D GT AIEGI +D L+ K +++     F  M NLR+LK+        + V     LE
Sbjct: 1144 FINDTGTSAIEGIFLDMLNLKFDAN--PNVFEKMCNLRLLKLYCSKAEEKHGVSFPQGLE 1203

Query: 183  YLSDQLRFLNWHGYPLKCLPSNFNPTNLLELELPSSCIDHLWKGPK--------SLDKLK 242
            YL  +LR L+W  YPL  LP +FNP NL+EL LPSSC   LWKG K        SL+KLK
Sbjct: 1204 YLPSKLRLLHWEYYPLSSLPKSFNPENLVELNLPSSCAKKLWKGKKARFCTTNSSLEKLK 1263

Query: 243  VINLSDSEFLSKTPDFSGVPNLERLVLSGCVRLFELHQSLGTLKHLIQLDLKDCKQLTSI 302
             + LS S+ L+K P  S   NLE + L GC  L  L QS+  LK L+ L+LK C +L +I
Sbjct: 1264 KMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLKGCSKLENI 1323

Query: 303  PFNISLESLNTLVLSGCWSLKSFPKISGNMNHLLELHLDGTSIKVLHPSMGHLTGLVLLN 362
            P  + LESL  L LSGC  L +FP+IS N+    EL++ GT I+ +  S+ +L  L  L+
Sbjct: 1324 PSMVDLESLEVLNLSGCSKLGNFPEISPNVK---ELYMGGTMIQEIPSSIKNLVLLEKLD 1383

Query: 363  LKNCKNLVKLPSTIGCLTSLKSLNLHGCSKLDSIPESLGNISCLEKLDVTGTCINEAPFS 415
            L+N ++L  LP++I  L  L++LNL GC  L+  P+S   + CL  LD++ T I E P S
Sbjct: 1384 LENSRHLKNLPTSIYKLKHLETLNLSGCISLERFPDSSRRMKCLRFLDLSRTDIKELPSS 1443