Lcy12g001280 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g001280 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 1.4e-137
Identity = 230/333 (69.07%), Postives = 271/333 (81.38%), Query Frame = 0

Query: 42  MVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGL 101
           +VPA+F+FGDSLIDNGNNNN+P+FA+ANYFPYGIDF  GPTGRF NG T+VD IA+LLGL
Sbjct: 52  IVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLGL 111

Query: 102 PLIPP-NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNL- 161
           PLIP  + A     LRG+NYASAA+GIL  TG NF+GRIPF+QQI NFE TLDQ+     
Sbjct: 112 PLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKSG 171

Query: 162 SATTVAPVVAGSIFFVGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYN 221
            A  +A  V  S+FF+GMGSNDYLNNYLMPN+PTR+QY S+QF DLL Q YT QL++LYN
Sbjct: 172 GAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQLTRLYN 231

Query: 222 LGARKFIIPGIGTMGCIPNILARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRF 281
           LG RKF++ G+G MGCIP+ILA+  DG+CSEEVNQLV  FN NV+TMI NLN NLP ++F
Sbjct: 232 LGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLPDAKF 291

Query: 282 TYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFH 341
            Y+DIA M EDI+AN  AYG T  D+GCCGIG+NRGQITCLPF+ PC NRDQYVFWDAFH
Sbjct: 292 IYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFWDAFH 351

Query: 342 PTERVNIIMARRAFNGGPTVAYPFNIQQLVDLD 373
           PTE+VN+IMA++AF G  TVAYP NIQQL  L+
Sbjct: 352 PTEKVNLIMAKKAFAGDRTVAYPINIQQLASLN 384

BLAST of Lcy12g001280 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.5e-97
Identity = 176/334 (52.69%), Postives = 224/334 (67.07%), Query Frame = 0

Query: 41  AMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLG 100
           A VPAMF+ GDSL+D GNNN L T ARAN+ PYGID    PTGRFSNG T +D +A LL 
Sbjct: 37  ARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLE 96

Query: 101 LPLIPPNTAPASGP---LRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITG 160
           +P  PP   P +     L+G+NYASAA+GILD++G N+ GR   NQQ+ N E TL Q+  
Sbjct: 97  IPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRT 156

Query: 161 NLSATTVAPVVAGSIFFVGMGSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQQYTQQLSK 220
            +S       +A S+  +  GSNDY+NNYLMPN Y +  ++    FA+LL  QY +QL  
Sbjct: 157 MMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLT 216

Query: 221 LYNLGARKFIIPGIGTMGCIPNILAR--TADGRCSEEVNQLVRDFNANVRTMIGNLNANL 280
           LY+LG RK  IPG+  +GCIPN  AR  +   RC + VNQ++  FN  +++++  LN   
Sbjct: 217 LYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQRS 276

Query: 281 PGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVF 340
           PG+ + Y +      DIL NP AYGF+V DR CCGIGRN+GQITCLP Q PC NR+QYVF
Sbjct: 277 PGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNRNQYVF 336

Query: 341 WDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQL 369
           WDAFHPT+  N I+ARRAF G P+ AYP N+QQ+
Sbjct: 337 WDAFHPTQTANSILARRAFYGPPSDAYPVNVQQM 370

BLAST of Lcy12g001280 vs. ExPASy Swiss-Prot
Match: Q9FK75 (GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.8e-95
Identity = 184/374 (49.20%), Postives = 242/374 (64.71%), Query Frame = 0

Query: 5   RLSSMWLAVLVMVIMLAAAATGQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPT 64
           R+S M + ++V V M+  A +                + P  FIFGDSL+DNGNNN L +
Sbjct: 3   RMSLMIMMIMVAVTMINIAKS--------------DPIAPCYFIFGDSLVDNGNNNQLQS 62

Query: 65  FARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLP--LIPPNTAPASGPLRGLNYAS 124
            ARANYFPYGIDFA GPTGRFSNG T VD IA+LLG    + P  +A     LRG+NYAS
Sbjct: 63  LARANYFPYGIDFAAGPTGRFSNGLTTVDVIAQLLGFEDYITPYASARGQDILRGVNYAS 122

Query: 125 AASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAP-VVAGSIFFVGMGSND 184
           AA+GI D TGR   GRI F  Q++N  NT+ Q+   L     A   ++  I+ +G+GSND
Sbjct: 123 AAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVVNILGDQNEASNYLSKCIYSIGLGSND 182

Query: 185 YLNNYLMPN-YPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNIL 244
           YLNNY MP  Y T +Q++   +AD L  +YT+QL  LY  GARKF + G+G +GC PN L
Sbjct: 183 YLNNYFMPTFYSTGNQFSPESYADDLVARYTEQLRVLYTNGARKFALIGVGAIGCSPNEL 242

Query: 245 ARTA-DGR-CSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAY 304
           A+ + DGR C E +N   R FN+ + +++   N N P ++FTY++   + +DI+ NP  Y
Sbjct: 243 AQNSRDGRTCDERINSANRIFNSKLISIVDAFNQNTPDAKFTYINAYGIFQDIITNPARY 302

Query: 305 GFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAF-NGGP 364
           GF VT+ GCCG+GRN GQITCLP Q PCLNR++YVFWDAFHP E  NI++ RR+F     
Sbjct: 303 GFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEYVFWDAFHPGEAANIVIGRRSFKREAA 362

Query: 365 TVAYPFNIQQLVDL 372
           + A+P++IQQL  L
Sbjct: 363 SDAHPYDIQQLASL 362

BLAST of Lcy12g001280 vs. ExPASy Swiss-Prot
Match: Q93YW8 (GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.3e-94
Identity = 175/341 (51.32%), Postives = 229/341 (67.16%), Query Frame = 0

Query: 38  MRPAMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAE 97
           M   + P  FIFGDSL+D+GNNN L + ARANYFPYGIDF  GPTGRFSNG T VD I E
Sbjct: 21  MGDPIAPCYFIFGDSLVDSGNNNRLTSLARANYFPYGIDFQYGPTGRFSNGKTTVDVITE 80

Query: 98  LLGLP--LIPPNTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQI 157
           LLG    + P + A     LRG+NYASAA+GI + TGR    RI F  Q++N  NT+ Q+
Sbjct: 81  LLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVSQV 140

Query: 158 TGNL-SATTVAPVVAGSIFFVGMGSNDYLNNYLMP-NYPTRSQYTSRQFADLLTQQYTQQ 217
              L      A  ++  I+ +G+GSNDYLNNY MP  Y T SQY+   +A+ L  +YT+Q
Sbjct: 141 VNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYTEQ 200

Query: 218 LSKLYNLGARKFIIPGIGTMGCIPNILARTA-DG-RCSEEVNQLVRDFNANVRTMIGNLN 277
           L  +YN GARKF + GIG +GC PN LA+ + DG  C E +N   R FN+ + +++ + N
Sbjct: 201 LRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDHFN 260

Query: 278 ANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQ 337
            N PG++FTY++   + +D++ANP  YGF VT+ GCCG+GRN GQITCLP Q PCLNRD+
Sbjct: 261 QNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRDE 320

Query: 338 YVFWDAFHPTERVNIIMARRAF-NGGPTVAYPFNIQQLVDL 372
           YVFWDAFHP E  N+++  R+F     + A+P++IQQL  L
Sbjct: 321 YVFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLARL 361

BLAST of Lcy12g001280 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.2e-93
Identity = 174/337 (51.63%), Postives = 226/337 (67.06%), Query Frame = 0

Query: 41  AMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLG 100
           A VP  F+FGDSL+DNGNNN L + AR+NYFPYGIDF  GPTGRFSNG T VD IAELLG
Sbjct: 28  AQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDFG-GPTGRFSNGKTTVDVIAELLG 87

Query: 101 LPLIPP--NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGN 160
                P  NT      L G+NYASAA+GI + TGR    RI F+ Q+ N++ T+ Q+   
Sbjct: 88  FNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQVVQL 147

Query: 161 L-SATTVAPVVAGSIFFVGMGSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQQYTQQLSK 220
           L   T  A  +   I+ VG+GSNDYLNNY MP  Y +  Q+T  Q+A+ L  +Y+ QL+ 
Sbjct: 148 LGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTPEQYANDLISRYSTQLNA 207

Query: 221 LYNLGARKFIIPGIGTMGCIPNILARTADGR-CSEEVNQLVRDFNANVRTMIGNLNANLP 280
           LYN GARKF + GIG +GC PN LA + DGR C + +N   + FN  +R+++  LN N P
Sbjct: 208 LYNYGARKFALSGIGAVGCSPNALAGSPDGRTCVDRINSANQIFNNKLRSLVDQLNNNHP 267

Query: 281 GSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFW 340
            ++F Y++   + +D++ NP  +GF VT+ GCCGIGRN GQITCLP Q PC +R+ YVFW
Sbjct: 268 DAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIGRNAGQITCLPGQRPCRDRNAYVFW 327

Query: 341 DAFHPTERVNIIMARRAFNG-GPTVAYPFNIQQLVDL 372
           DAFHPTE  N+I+ARR++N    + AYP +I +L  L
Sbjct: 328 DAFHPTEAANVIIARRSYNAQSASDAYPMDISRLAQL 363

BLAST of Lcy12g001280 vs. ExPASy TrEMBL
Match: A0A6J1IA39 (GDSL esterase/lipase At1g71691-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472592 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 6.8e-191
Identity = 333/373 (89.28%), Postives = 350/373 (93.83%), Query Frame = 0

Query: 1   MAELRLSSMWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR +SM L VLV++++ AAAA  GQNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFTSMLLPVLVLLLVAAAAAAFGQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLN 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ APA+GPLRGLN
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPATGPLRGLN 120

Query: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGS 180
           YASAASGILDITGRNFIGRIPFNQQI NFENTLDQITGNL A TVAPVVA  IFFVGMGS
Sbjct: 121 YASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMGS 180

Query: 181 NDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNI 240
           NDYLNNYLMPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+
Sbjct: 181 NDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNV 240

Query: 241 LARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYG 300
           LAR++DGRCSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAYG
Sbjct: 241 LARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAYG 300

Query: 301 FTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTV 360
           FTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +V
Sbjct: 301 FTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSV 360

Query: 361 AYPFNIQQLVDLD 373
           AYPFNIQQL +LD
Sbjct: 361 AYPFNIQQLANLD 371

BLAST of Lcy12g001280 vs. ExPASy TrEMBL
Match: A0A6J1EBS6 (GDSL esterase/lipase At1g71691-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432760 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 333/373 (89.28%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MAELRLSSMWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SSM L VLV++++ AAAA   QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLN 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ AP +GPLRGLN
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPTTGPLRGLN 120

Query: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGS 180
           YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNL A TVAPVVA  IFFVGMGS
Sbjct: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLGAATVAPVVARCIFFVGMGS 180

Query: 181 NDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNI 240
           NDYLNNYLMPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+
Sbjct: 181 NDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNV 240

Query: 241 LARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYG 300
           LAR++DGRCSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAYG
Sbjct: 241 LARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAYG 300

Query: 301 FTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTV 360
           FTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +V
Sbjct: 301 FTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSV 360

Query: 361 AYPFNIQQLVDLD 373
           AYPFNIQQL +LD
Sbjct: 361 AYPFNIQQLANLD 371

BLAST of Lcy12g001280 vs. ExPASy TrEMBL
Match: A0A6J1IEV3 (GDSL esterase/lipase At1g71691-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472592 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.1e-184
Identity = 324/365 (88.77%), Postives = 340/365 (93.15%), Query Frame = 0

Query: 9   MWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPTFAR 68
           M L VLV++++ AAAA  GQNGST GGGG  RPAMVPAMFIFGDSLIDNGNNNNLPTFAR
Sbjct: 1   MLLPVLVLLLVAAAAAAFGQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNNNNLPTFAR 60

Query: 69  ANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLNYASAASGI 128
           ANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ APA+GPLRGLNYASAASGI
Sbjct: 61  ANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPATGPLRGLNYASAASGI 120

Query: 129 LDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGSNDYLNNYL 188
           LDITGRNFIGRIPFNQQI NFENTLDQITGNL A TVAPVVA  IFFVGMGSNDYLNNYL
Sbjct: 121 LDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMGSNDYLNNYL 180

Query: 189 MPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNILARTADGR 248
           MPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+LAR++DGR
Sbjct: 181 MPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNVLARSSDGR 240

Query: 249 CSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGC 308
           CSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAY   V DRGC
Sbjct: 241 CSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAY---VADRGC 300

Query: 309 CGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQ 368
           CGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +VAYPFNIQQ
Sbjct: 301 CGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSVAYPFNIQQ 360

Query: 369 LVDLD 373
           L +LD
Sbjct: 361 LANLD 360

BLAST of Lcy12g001280 vs. ExPASy TrEMBL
Match: A0A6J1CJ89 (GDSL esterase/lipase At1g71691-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012108 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.8e-184
Identity = 322/374 (86.10%), Postives = 341/374 (91.18%), Query Frame = 0

Query: 1   MAELRLSSMWLAVLVMVIMLAAAATGQNGSTY--GGGGGMRPAMVPAMFIFGDSLIDNGN 60
           MAELRLSSM +AVL MVI+LAAAA GQN STY  GGGGG R  MVPAMFIFGDSLIDNGN
Sbjct: 1   MAELRLSSM-VAVLAMVILLAAAAPGQNESTYGGGGGGGWRRPMVPAMFIFGDSLIDNGN 60

Query: 61  NNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGL 120
           NNNLPTFARANYFPYGIDF  GPTGRFSNGYTIVDEIA+LLGLPLIPP+TAP  GPLRGL
Sbjct: 61  NNNLPTFARANYFPYGIDFVGGPTGRFSNGYTIVDEIAKLLGLPLIPPSTAPVMGPLRGL 120

Query: 121 NYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMG 180
           NYASAASGILDITGRNFIGRIPFNQQI NFENTLDQITGNL A TVAPVVA  IFFVGMG
Sbjct: 121 NYASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMG 180

Query: 181 SNDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPN 240
           SNDYLNNYLMPNYPTR QYTSRQFADLL Q+YTQQL++LYNLGARKF+IPGIG MGCIPN
Sbjct: 181 SNDYLNNYLMPNYPTRFQYTSRQFADLLVQRYTQQLTRLYNLGARKFVIPGIGIMGCIPN 240

Query: 241 ILARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAY 300
           +LAR++DGRC EEVNQL RDFNANVRTMI NLN NLPGSRFTY++IAR+ +DILANP AY
Sbjct: 241 MLARSSDGRCLEEVNQLGRDFNANVRTMIDNLNTNLPGSRFTYINIARLFQDILANPMAY 300

Query: 301 GFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPT 360
           GFTV D GCCGIGRNRGQITCLPFQMPCLNR+QYVFWDAFHPT+RVNIIMARRAFNG  T
Sbjct: 301 GFTVADHGCCGIGRNRGQITCLPFQMPCLNREQYVFWDAFHPTQRVNIIMARRAFNGDQT 360

Query: 361 VAYPFNIQQLVDLD 373
           VAYPFNIQ+L  L+
Sbjct: 361 VAYPFNIQRLATLN 373

BLAST of Lcy12g001280 vs. ExPASy TrEMBL
Match: A0A6J1EF98 (GDSL esterase/lipase At1g71691-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432760 PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.1e-184
Identity = 323/365 (88.49%), Postives = 339/365 (92.88%), Query Frame = 0

Query: 9   MWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPTFAR 68
           M L VLV++++ AAAA   QNGST GGGG  RPAMVPAMFIFGDSLIDNGNNNNLPTFAR
Sbjct: 1   MLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNNNNLPTFAR 60

Query: 69  ANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLNYASAASGI 128
           ANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ AP +GPLRGLNYASAASGI
Sbjct: 61  ANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPTTGPLRGLNYASAASGI 120

Query: 129 LDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGSNDYLNNYL 188
           LDITGRNFIGRIPFNQQISNFENTLDQITGNL A TVAPVVA  IFFVGMGSNDYLNNYL
Sbjct: 121 LDITGRNFIGRIPFNQQISNFENTLDQITGNLGAATVAPVVARCIFFVGMGSNDYLNNYL 180

Query: 189 MPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNILARTADGR 248
           MPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+LAR++DGR
Sbjct: 181 MPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNVLARSSDGR 240

Query: 249 CSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGC 308
           CSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAY   V DRGC
Sbjct: 241 CSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAY---VADRGC 300

Query: 309 CGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQ 368
           CGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +VAYPFNIQQ
Sbjct: 301 CGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSVAYPFNIQQ 360

Query: 369 LVDLD 373
           L +LD
Sbjct: 361 LANLD 360

BLAST of Lcy12g001280 vs. NCBI nr
Match: XP_022973976.1 (GDSL esterase/lipase At1g71691-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 676.4 bits (1744), Expect = 1.4e-190
Identity = 333/373 (89.28%), Postives = 350/373 (93.83%), Query Frame = 0

Query: 1   MAELRLSSMWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR +SM L VLV++++ AAAA  GQNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFTSMLLPVLVLLLVAAAAAAFGQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLN 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ APA+GPLRGLN
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPATGPLRGLN 120

Query: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGS 180
           YASAASGILDITGRNFIGRIPFNQQI NFENTLDQITGNL A TVAPVVA  IFFVGMGS
Sbjct: 121 YASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMGS 180

Query: 181 NDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNI 240
           NDYLNNYLMPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+
Sbjct: 181 NDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNV 240

Query: 241 LARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYG 300
           LAR++DGRCSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAYG
Sbjct: 241 LARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAYG 300

Query: 301 FTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTV 360
           FTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +V
Sbjct: 301 FTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSV 360

Query: 361 AYPFNIQQLVDLD 373
           AYPFNIQQL +LD
Sbjct: 361 AYPFNIQQLANLD 371

BLAST of Lcy12g001280 vs. NCBI nr
Match: XP_022925467.1 (GDSL esterase/lipase At1g71691-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 675.6 bits (1742), Expect = 2.4e-190
Identity = 333/373 (89.28%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MAELRLSSMWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SSM L VLV++++ AAAA   QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLN 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ AP +GPLRGLN
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPTTGPLRGLN 120

Query: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGS 180
           YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNL A TVAPVVA  IFFVGMGS
Sbjct: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLGAATVAPVVARCIFFVGMGS 180

Query: 181 NDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNI 240
           NDYLNNYLMPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+
Sbjct: 181 NDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNV 240

Query: 241 LARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYG 300
           LAR++DGRCSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAYG
Sbjct: 241 LARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAYG 300

Query: 301 FTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTV 360
           FTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +V
Sbjct: 301 FTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSV 360

Query: 361 AYPFNIQQLVDLD 373
           AYPFNIQQL +LD
Sbjct: 361 AYPFNIQQLANLD 371

BLAST of Lcy12g001280 vs. NCBI nr
Match: XP_023535504.1 (GDSL esterase/lipase At1g71691-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674.1 bits (1738), Expect = 7.0e-190
Identity = 332/373 (89.01%), Postives = 348/373 (93.30%), Query Frame = 0

Query: 1   MAELRLSSMWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SSM L VLV++++ AAAA   QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLN 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ AP +GPLRGLN
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPTTGPLRGLN 120

Query: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGS 180
           YASAASGILDITGRNFIGRIPFNQQI NFENTLDQITGNL A TVAPVVA  IFFVGMGS
Sbjct: 121 YASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMGS 180

Query: 181 NDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNI 240
           NDYLNNYLMPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+
Sbjct: 181 NDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNV 240

Query: 241 LARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYG 300
           LAR++DGRCSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAYG
Sbjct: 241 LARSSDGRCSEEVSQLVRDFNANVRVMIGNLNANLPGSRFTYVNIARMNGDILANPRAYG 300

Query: 301 FTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTV 360
           FTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +V
Sbjct: 301 FTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSV 360

Query: 361 AYPFNIQQLVDLD 373
           AYPFNIQQL +LD
Sbjct: 361 AYPFNIQQLANLD 371

BLAST of Lcy12g001280 vs. NCBI nr
Match: KAG6592374.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.8 bits (1732), Expect = 3.5e-189
Identity = 331/373 (88.74%), Postives = 347/373 (93.03%), Query Frame = 0

Query: 1   MAELRLSSMWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SSM L VLV++++ AAAA   QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 60  MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 119

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLN 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ AP +GPLRGLN
Sbjct: 120 NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPTTGPLRGLN 179

Query: 121 YASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGS 180
           YASAASGILDITGRNFIGRIPFNQQI NFENTLDQITGNL A TVAPVVA  IFFVGMGS
Sbjct: 180 YASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMGS 239

Query: 181 NDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNI 240
           NDYLNNYLMPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+
Sbjct: 240 NDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNV 299

Query: 241 LARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYG 300
           LAR++DGRCSEEV QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAYG
Sbjct: 300 LARSSDGRCSEEVRQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAYG 359

Query: 301 FTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTV 360
           FTV DRGCCGIGRNRGQITCLP+QMPCLNRD+YVFWDAFHPTERVNIIMARRAFNG  +V
Sbjct: 360 FTVADRGCCGIGRNRGQITCLPYQMPCLNRDRYVFWDAFHPTERVNIIMARRAFNGDMSV 419

Query: 361 AYPFNIQQLVDLD 373
           AYPFNIQQL +LD
Sbjct: 420 AYPFNIQQLANLD 430

BLAST of Lcy12g001280 vs. NCBI nr
Match: XP_022973978.1 (GDSL esterase/lipase At1g71691-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 654.8 bits (1688), Expect = 4.4e-184
Identity = 324/365 (88.77%), Postives = 340/365 (93.15%), Query Frame = 0

Query: 9   MWLAVLVMVIMLAAAAT-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPTFAR 68
           M L VLV++++ AAAA  GQNGST GGGG  RPAMVPAMFIFGDSLIDNGNNNNLPTFAR
Sbjct: 1   MLLPVLVLLLVAAAAAAFGQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNNNNLPTFAR 60

Query: 69  ANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLPLIPPNTAPASGPLRGLNYASAASGI 128
           ANYFPYGIDFADGPTGRFSNGYTIVDEIA+LLGLPLIPP+ APA+GPLRGLNYASAASGI
Sbjct: 61  ANYFPYGIDFADGPTGRFSNGYTIVDEIAKLLGLPLIPPSAAPATGPLRGLNYASAASGI 120

Query: 129 LDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAPVVAGSIFFVGMGSNDYLNNYL 188
           LDITGRNFIGRIPFNQQI NFENTLDQITGNL A TVAPVVA  IFFVGMGSNDYLNNYL
Sbjct: 121 LDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMGSNDYLNNYL 180

Query: 189 MPNYPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNILARTADGR 248
           MPNYPTRSQY SRQFADLLTQQYTQQLS+LYNLGARKFIIPGIGTM CIPN+LAR++DGR
Sbjct: 181 MPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNVLARSSDGR 240

Query: 249 CSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGC 308
           CSEEV+QLVRDFNANVR MIGNLNANLPGSRFTYV+IARMN DILANPRAY   V DRGC
Sbjct: 241 CSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAY---VADRGC 300

Query: 309 CGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQ 368
           CGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +VAYPFNIQQ
Sbjct: 301 CGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSVAYPFNIQQ 360

Query: 369 LVDLD 373
           L +LD
Sbjct: 361 LANLD 360

BLAST of Lcy12g001280 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 490.7 bits (1262), Expect = 1.0e-138
Identity = 230/333 (69.07%), Postives = 271/333 (81.38%), Query Frame = 0

Query: 42  MVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGL 101
           +VPA+F+FGDSLIDNGNNNN+P+FA+ANYFPYGIDF  GPTGRF NG T+VD IA+LLGL
Sbjct: 52  IVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLGL 111

Query: 102 PLIPP-NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNL- 161
           PLIP  + A     LRG+NYASAA+GIL  TG NF+GRIPF+QQI NFE TLDQ+     
Sbjct: 112 PLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKSG 171

Query: 162 SATTVAPVVAGSIFFVGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQQLSKLYN 221
            A  +A  V  S+FF+GMGSNDYLNNYLMPN+PTR+QY S+QF DLL Q YT QL++LYN
Sbjct: 172 GAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQLTRLYN 231

Query: 222 LGARKFIIPGIGTMGCIPNILARTADGRCSEEVNQLVRDFNANVRTMIGNLNANLPGSRF 281
           LG RKF++ G+G MGCIP+ILA+  DG+CSEEVNQLV  FN NV+TMI NLN NLP ++F
Sbjct: 232 LGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLPDAKF 291

Query: 282 TYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFH 341
            Y+DIA M EDI+AN  AYG T  D+GCCGIG+NRGQITCLPF+ PC NRDQYVFWDAFH
Sbjct: 292 IYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFWDAFH 351

Query: 342 PTERVNIIMARRAFNGGPTVAYPFNIQQLVDLD 373
           PTE+VN+IMA++AF G  TVAYP NIQQL  L+
Sbjct: 352 PTEKVNLIMAKKAFAGDRTVAYPINIQQLASLN 384

BLAST of Lcy12g001280 vs. TAIR 10
Match: AT1G71691.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 396.0 bits (1016), Expect = 3.4e-110
Identity = 187/280 (66.79%), Postives = 222/280 (79.29%), Query Frame = 0

Query: 95  IAELLGLPLIPP-NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLD 154
           + +LLGLPLIP  + A     LRG+NYASAA+GIL  TG NF+GRIPF+QQI NFE TLD
Sbjct: 4   VTQLLGLPLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLD 63

Query: 155 QITGNL-SATTVAPVVAGSIFFVGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQQYTQ 214
           Q+      A  +A  V  S+FF+GMGSNDYLNNYLMPN+PTR+QY S+QF DLL Q YT 
Sbjct: 64  QVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTD 123

Query: 215 QLSKLYNLGARKFIIPGIGTMGCIPNILARTADGRCSEEVNQLVRDFNANVRTMIGNLNA 274
           QL++LYNLG RKF++ G+G MGCIP+ILA+  DG+CSEEVNQLV  FN NV+TMI NLN 
Sbjct: 124 QLTRLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQ 183

Query: 275 NLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQY 334
           NLP ++F Y+DIA M EDI+AN  AYG T  D+GCCGIG+NRGQITCLPF+ PC NRDQY
Sbjct: 184 NLPDAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQY 243

Query: 335 VFWDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQLVDLD 373
           VFWDAFHPTE+VN+IMA++AF G  TVAYP NIQQL  L+
Sbjct: 244 VFWDAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQLASLN 283

BLAST of Lcy12g001280 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 357.1 bits (915), Expect = 1.8e-98
Identity = 176/334 (52.69%), Postives = 224/334 (67.07%), Query Frame = 0

Query: 41  AMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLG 100
           A VPAMF+ GDSL+D GNNN L T ARAN+ PYGID    PTGRFSNG T +D +A LL 
Sbjct: 37  ARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLE 96

Query: 101 LPLIPPNTAPASGP---LRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITG 160
           +P  PP   P +     L+G+NYASAA+GILD++G N+ GR   NQQ+ N E TL Q+  
Sbjct: 97  IPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRT 156

Query: 161 NLSATTVAPVVAGSIFFVGMGSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQQYTQQLSK 220
            +S       +A S+  +  GSNDY+NNYLMPN Y +  ++    FA+LL  QY +QL  
Sbjct: 157 MMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLT 216

Query: 221 LYNLGARKFIIPGIGTMGCIPNILAR--TADGRCSEEVNQLVRDFNANVRTMIGNLNANL 280
           LY+LG RK  IPG+  +GCIPN  AR  +   RC + VNQ++  FN  +++++  LN   
Sbjct: 217 LYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQRS 276

Query: 281 PGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVF 340
           PG+ + Y +      DIL NP AYGF+V DR CCGIGRN+GQITCLP Q PC NR+QYVF
Sbjct: 277 PGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNRNQYVF 336

Query: 341 WDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQL 369
           WDAFHPT+  N I+ARRAF G P+ AYP N+QQ+
Sbjct: 337 WDAFHPTQTANSILARRAFYGPPSDAYPVNVQQM 370

BLAST of Lcy12g001280 vs. TAIR 10
Match: AT5G45670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.3e-96
Identity = 184/374 (49.20%), Postives = 242/374 (64.71%), Query Frame = 0

Query: 5   RLSSMWLAVLVMVIMLAAAATGQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPT 64
           R+S M + ++V V M+  A +                + P  FIFGDSL+DNGNNN L +
Sbjct: 3   RMSLMIMMIMVAVTMINIAKS--------------DPIAPCYFIFGDSLVDNGNNNQLQS 62

Query: 65  FARANYFPYGIDFADGPTGRFSNGYTIVDEIAELLGLP--LIPPNTAPASGPLRGLNYAS 124
            ARANYFPYGIDFA GPTGRFSNG T VD IA+LLG    + P  +A     LRG+NYAS
Sbjct: 63  LARANYFPYGIDFAAGPTGRFSNGLTTVDVIAQLLGFEDYITPYASARGQDILRGVNYAS 122

Query: 125 AASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLSATTVAP-VVAGSIFFVGMGSND 184
           AA+GI D TGR   GRI F  Q++N  NT+ Q+   L     A   ++  I+ +G+GSND
Sbjct: 123 AAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVVNILGDQNEASNYLSKCIYSIGLGSND 182

Query: 185 YLNNYLMPN-YPTRSQYTSRQFADLLTQQYTQQLSKLYNLGARKFIIPGIGTMGCIPNIL 244
           YLNNY MP  Y T +Q++   +AD L  +YT+QL  LY  GARKF + G+G +GC PN L
Sbjct: 183 YLNNYFMPTFYSTGNQFSPESYADDLVARYTEQLRVLYTNGARKFALIGVGAIGCSPNEL 242

Query: 245 ARTA-DGR-CSEEVNQLVRDFNANVRTMIGNLNANLPGSRFTYVDIARMNEDILANPRAY 304
           A+ + DGR C E +N   R FN+ + +++   N N P ++FTY++   + +DI+ NP  Y
Sbjct: 243 AQNSRDGRTCDERINSANRIFNSKLISIVDAFNQNTPDAKFTYINAYGIFQDIITNPARY 302

Query: 305 GFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAF-NGGP 364
           GF VT+ GCCG+GRN GQITCLP Q PCLNR++YVFWDAFHP E  NI++ RR+F     
Sbjct: 303 GFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEYVFWDAFHPGEAANIVIGRRSFKREAA 362

Query: 365 TVAYPFNIQQLVDL 372
           + A+P++IQQL  L
Sbjct: 363 SDAHPYDIQQLASL 362

BLAST of Lcy12g001280 vs. TAIR 10
Match: AT4G18970.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.4e-95
Identity = 175/341 (51.32%), Postives = 229/341 (67.16%), Query Frame = 0

Query: 38  MRPAMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIAE 97
           M   + P  FIFGDSL+D+GNNN L + ARANYFPYGIDF  GPTGRFSNG T VD I E
Sbjct: 21  MGDPIAPCYFIFGDSLVDSGNNNRLTSLARANYFPYGIDFQYGPTGRFSNGKTTVDVITE 80

Query: 98  LLGLP--LIPPNTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQI 157
           LLG    + P + A     LRG+NYASAA+GI + TGR    RI F  Q++N  NT+ Q+
Sbjct: 81  LLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVSQV 140

Query: 158 TGNL-SATTVAPVVAGSIFFVGMGSNDYLNNYLMP-NYPTRSQYTSRQFADLLTQQYTQQ 217
              L      A  ++  I+ +G+GSNDYLNNY MP  Y T SQY+   +A+ L  +YT+Q
Sbjct: 141 VNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYTEQ 200

Query: 218 LSKLYNLGARKFIIPGIGTMGCIPNILARTA-DG-RCSEEVNQLVRDFNANVRTMIGNLN 277
           L  +YN GARKF + GIG +GC PN LA+ + DG  C E +N   R FN+ + +++ + N
Sbjct: 201 LRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDHFN 260

Query: 278 ANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQ 337
            N PG++FTY++   + +D++ANP  YGF VT+ GCCG+GRN GQITCLP Q PCLNRD+
Sbjct: 261 QNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRDE 320

Query: 338 YVFWDAFHPTERVNIIMARRAF-NGGPTVAYPFNIQQLVDL 372
           YVFWDAFHP E  N+++  R+F     + A+P++IQQL  L
Sbjct: 321 YVFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLARL 361