Lcy11g019460 (gene) Sponge gourd (P93075) v1

Overview
NameLcy11g019460
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr11: 46911816 .. 46912301 (-)
RNA-Seq ExpressionLcy11g019460
SyntenyLcy11g019460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGCAAGGAACCAAGCGAGTCAGGCTCGGTGGGAGAGACCAATCGCGAACCAGTGGAAAATGAACACGGATGCCGCCTGGAACGAGAGAGAAGGCCGTGACGGGATTGGATGGGTCGTGCGTGACTCGAATGGATCCCTGATTTGCTTCAGAATGAAACCAATCAACAAAAATTGGTCTATAAAAATGCTAGAAACAGAGCCGATTCTAGAAGGACTCAAATCAATTATTGATACCTGCAAACAATCTCTCATCCCTTTGATTGTAGAATCGGACGTGTTGGAAGTTGTTAGGTTGCTGAACGGAGAAGATGAGGACCTGTCGGACGTGAAGACCATTTCAGACGCCATCTCCGCCCTCGCCTCCAATGCTCCAGCCGTCAATTTTGTTCATTGTAGTAGATTTTTTAACACAGTAGCGCACTGTGTTGCCAAGGAGGCTTGTAATCCCTATTTTTTTTGTCGATCGAACAATGGATTCTAG

mRNA sequence

ATGTCAGCAAGGAACCAAGCGAGTCAGGCTCGGTGGGAGAGACCAATCGCGAACCAGTGGAAAATGAACACGGATGCCGCCTGGAACGAGAGAGAAGGCCGTGACGGGATTGGATGGGTCGTGCGTGACTCGAATGGATCCCTGATTTGCTTCAGAATGAAACCAATCAACAAAAATTGGTCTATAAAAATGCTAGAAACAGAGCCGATTCTAGAAGGACTCAAATCAATTATTGATACCTGCAAACAATCTCTCATCCCTTTGATTGTAGAATCGGACGTGTTGGAAGTTGTTAGGTTGCTGAACGGAGAAGATGAGGACCTGTCGGACGTGAAGACCATTTCAGACGCCATCTCCGCCCTCGCCTCCAATGCTCCAGCCGTCAATTTTGTTCATTGTAGTAGATTTTTTAACACAGTAGCGCACTGTGTTGCCAAGGAGGCTTGTAATCCCTATTTTTTTTGTCGATCGAACAATGGATTCTAG

Coding sequence (CDS)

ATGTCAGCAAGGAACCAAGCGAGTCAGGCTCGGTGGGAGAGACCAATCGCGAACCAGTGGAAAATGAACACGGATGCCGCCTGGAACGAGAGAGAAGGCCGTGACGGGATTGGATGGGTCGTGCGTGACTCGAATGGATCCCTGATTTGCTTCAGAATGAAACCAATCAACAAAAATTGGTCTATAAAAATGCTAGAAACAGAGCCGATTCTAGAAGGACTCAAATCAATTATTGATACCTGCAAACAATCTCTCATCCCTTTGATTGTAGAATCGGACGTGTTGGAAGTTGTTAGGTTGCTGAACGGAGAAGATGAGGACCTGTCGGACGTGAAGACCATTTCAGACGCCATCTCCGCCCTCGCCTCCAATGCTCCAGCCGTCAATTTTGTTCATTGTAGTAGATTTTTTAACACAGTAGCGCACTGTGTTGCCAAGGAGGCTTGTAATCCCTATTTTTTTTGTCGATCGAACAATGGATTCTAG

Protein sequence

MSARNQASQARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETEPILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPAVNFVHCSRFFNTVAHCVAKEACNPYFFCRSNNGF
Homology
BLAST of Lcy11g019460 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.0e-14
Identity = 51/144 (35.42%), Postives = 80/144 (55.56%), Query Frame = 0

Query: 10  ARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETEP 69
           ARW+ P +N WK+NTDAAW       GIGW++RD  G +I    + I    +I  LE   
Sbjct: 77  ARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMA 136

Query: 70  ILEGLKSI-IDTCK----QSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASN 129
           I EGL++I  + C+    +   P+ +ESD LE + LL+ + +D +++  + + I  +  +
Sbjct: 137 ICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMED 196

Query: 130 APAVNFVHCSRFFNTVAHCVAKEA 149
              V+  H SR  N VAH +A+ A
Sbjct: 197 MKIVSMRHISREANKVAHDLARRA 220

BLAST of Lcy11g019460 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 5.8e-14
Identity = 48/139 (34.53%), Postives = 78/139 (56.12%), Query Frame = 0

Query: 10  ARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETEP 69
           A+W+ P +N WK+NT+AAW       GIGW++RD  G +I    + I    +I  LE   
Sbjct: 77  AQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMA 136

Query: 70  ILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPAVN 129
           I EGL++I    ++   P+ +ESD LE + LL+ + +D +++  + + I  +  +   V+
Sbjct: 137 ICEGLRAI---RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVS 196

Query: 130 FVHCSRFFNTVAHCVAKEA 149
             H SR  N VAH +A+ A
Sbjct: 197 MRHISREANKVAHGLARRA 212

BLAST of Lcy11g019460 vs. ExPASy TrEMBL
Match: A0A5C7H0P0 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.9e-12
Identity = 46/140 (32.86%), Postives = 79/140 (56.43%), Query Frame = 0

Query: 9   QARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETE 68
           Q  W+ P   ++K+N DA++  R G+ G+G ++RD  GS I  R  P+    S++MLE +
Sbjct: 270 QPSWKTPKRGEFKINCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLEAQ 329

Query: 69  PILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPAV 128
             LEG+   ID     +  +I+ESD   V++LL+ +    +++  I     AL ++   +
Sbjct: 330 ACLEGIHLAIDI---GVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLL 389

Query: 129 NFVHCSRFFNTVAHCVAKEA 149
           ++V   R  N+VAHC+A+ A
Sbjct: 390 SYVAVRREANSVAHCIAQHA 406

BLAST of Lcy11g019460 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.2e-12
Identity = 46/141 (32.62%), Postives = 77/141 (54.61%), Query Frame = 0

Query: 8   SQARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLET 67
           ++ +WE P  + W +N DA+W++   R GIGW++R  +G ++    + +    ++K+LE 
Sbjct: 67  NKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEA 126

Query: 68  EPILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPA 127
             ILEGL+++  T    L PL +E+D  EV  LLN + EDL+    + + I  L  +   
Sbjct: 127 SAILEGLRNL--TNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEI 186

Query: 128 VNFVHCSRFFNTVAHCVAKEA 149
           + F    R  N  AH +A+ A
Sbjct: 187 LAFAKVGRETNGCAHSLAQRA 205

BLAST of Lcy11g019460 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 7.9e-11
Identity = 39/95 (41.05%), Postives = 57/95 (60.00%), Query Frame = 0

Query: 8   SQARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLET 67
           ++ARW+ P +N WK+NTDAAW      DGIGW++RD  G +I    + I    +I  LE 
Sbjct: 251 TRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEV 310

Query: 68  EPILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLN 103
             I EGL++I    ++   P+ +ESD LE + LL+
Sbjct: 311 MAICEGLRAI---RQEHCRPIHLESDSLEAIHLLH 342

BLAST of Lcy11g019460 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 89.0 bits (219), Expect = 4.1e-14
Identity = 51/144 (35.42%), Postives = 80/144 (55.56%), Query Frame = 0

Query: 10  ARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETEP 69
           ARW+ P +N WK+NTDAAW       GIGW++RD  G +I    + I    +I  LE   
Sbjct: 77  ARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMA 136

Query: 70  ILEGLKSI-IDTCK----QSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASN 129
           I EGL++I  + C+    +   P+ +ESD LE + LL+ + +D +++  + + I  +  +
Sbjct: 137 ICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMED 196

Query: 130 APAVNFVHCSRFFNTVAHCVAKEA 149
              V+  H SR  N VAH +A+ A
Sbjct: 197 MKIVSMRHISREANKVAHDLARRA 220

BLAST of Lcy11g019460 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-13
Identity = 48/139 (34.53%), Postives = 78/139 (56.12%), Query Frame = 0

Query: 10  ARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETEP 69
           A+W+ P +N WK+NT+AAW       GIGW++RD  G +I    + I    +I  LE   
Sbjct: 77  AQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMA 136

Query: 70  ILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPAVN 129
           I EGL++I    ++   P+ +ESD LE + LL+ + +D +++  + + I  +  +   V+
Sbjct: 137 ICEGLRAI---RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVS 196

Query: 130 FVHCSRFFNTVAHCVAKEA 149
             H SR  N VAH +A+ A
Sbjct: 197 MRHISREANKVAHGLARRA 212

BLAST of Lcy11g019460 vs. NCBI nr
Match: TXG50387.1 (hypothetical protein EZV62_022911 [Acer yangbiense])

HSP 1 Score: 82.4 bits (202), Expect = 3.9e-12
Identity = 46/140 (32.86%), Postives = 79/140 (56.43%), Query Frame = 0

Query: 9   QARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETE 68
           Q  W+ P   ++K+N DA++  R G+ G+G ++RD  GS I  R  P+    S++MLE +
Sbjct: 270 QPSWKTPKRGEFKINCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLEAQ 329

Query: 69  PILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPAV 128
             LEG+   ID     +  +I+ESD   V++LL+ +    +++  I     AL ++   +
Sbjct: 330 ACLEGIHLAIDI---GVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLL 389

Query: 129 NFVHCSRFFNTVAHCVAKEA 149
           ++V   R  N+VAHC+A+ A
Sbjct: 390 SYVAVRREANSVAHCIAQHA 406

BLAST of Lcy11g019460 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 81.6 bits (200), Expect = 6.6e-12
Identity = 46/141 (32.62%), Postives = 77/141 (54.61%), Query Frame = 0

Query: 8   SQARWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLET 67
           ++ +WE P  + W +N DA+W++   R GIGW++R  +G ++    + +    ++K+LE 
Sbjct: 67  NKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEA 126

Query: 68  EPILEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPA 127
             ILEGL+++  T    L PL +E+D  EV  LLN + EDL+    + + I  L  +   
Sbjct: 127 SAILEGLRNL--TNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEI 186

Query: 128 VNFVHCSRFFNTVAHCVAKEA 149
           + F    R  N  AH +A+ A
Sbjct: 187 LAFAKVGRETNGCAHSLAQRA 205

BLAST of Lcy11g019460 vs. NCBI nr
Match: KAG6599977.1 (hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-10
Identity = 42/113 (37.17%), Postives = 65/113 (57.52%), Query Frame = 0

Query: 21  KMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETEPILEGLKS---I 80
           K+N+ A+W+E  G+ G+ WV+ DS GS IC   K + + W +KMLE + ++EGLK+   I
Sbjct: 7   KLNSYASWSEEAGKGGVSWVIHDSLGSSICIECKELRRKWLVKMLEGKAMIEGLKTYLLI 66

Query: 81  IDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPAVNF 131
            +T   S  P+I     LE+ R+LN    DL ++  + D I  L   A  ++F
Sbjct: 67  RETVSYSHRPII----FLELARILNNTHMDLIELSNVVDEIFDLELLAGIISF 115

BLAST of Lcy11g019460 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 7.8e-11
Identity = 52/146 (35.62%), Postives = 77/146 (52.74%), Query Frame = 0

Query: 5   NQASQARWERPIANQW-KMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIK 64
           N++S  RW RP  +QW K NTDA WN    R GIGWV+R+  G +     + + K  S+ 
Sbjct: 413 NRSSCGRW-RPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSV- 472

Query: 65  MLETEPILEGLK-SIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALA 124
            LE E  LE ++ +++   +     +I ESD   ++ +LN ++   S   TI D +  L 
Sbjct: 473 -LEAE--LEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNNDEIWPSLKPTIQD-LQRLL 532

Query: 125 SNAPAVNFVHCSRFFNTVAHCVAKEA 149
           S    V FV   R  NT+A  VA+E+
Sbjct: 533 SQFTEVKFVFIPREGNTLAERVARES 552

BLAST of Lcy11g019460 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-07
Identity = 45/141 (31.91%), Postives = 69/141 (48.94%), Query Frame = 0

Query: 11  RWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICF--RMKPINKNWSIKMLETE 70
           +W+ P     K NTDA W     R GIGW++R+ +G ++    R  P  KN    +LE E
Sbjct: 136 QWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKN----VLEAE 195

Query: 71  PILEGLK-SIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPA 130
             LE L+ +++   + +   +I ESD   +V LLN +D   +    + D I  L  +   
Sbjct: 196 --LEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDFWPTLQPALED-IQQLLHHFEE 255

Query: 131 VNFVHCSRFFNTVAHCVAKEA 149
           V F    R  N VA  +A+E+
Sbjct: 256 VKFEFTPRGGNKVADRIARES 269

BLAST of Lcy11g019460 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 41.2 bits (95), Expect = 9.2e-04
Identity = 31/138 (22.46%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 11  RWERPIANQWKMNTDAAWNEREGRDGIGWVVRDSNGSLICFRMKPINKNWSIKMLETEPI 70
           +W  P  ++ K N DA+ +ER    G+GW++R+S G++I   M       + +  E   +
Sbjct: 104 KWSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTL 163

Query: 71  LEGLKSIIDTCKQSLIPLIVESDVLEVVRLLNGEDEDLSDVKTISDAISALASNAPAVNF 130
           +  +++      +    +I E D   + R++N +  +   ++   D I +   +  ++ F
Sbjct: 164 IWAIQASYGFGHKK---VIFEGDNQTITRMINTKSSN-PRLQHFLDTIQSWIPSFESIEF 223

Query: 131 VHCSRFFNTVAHCVAKEA 149
               R  N  A  +AK+A
Sbjct: 224 SFKHREQNGCADFLAKQA 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DSV12.0e-1435.42uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1CP265.8e-1434.53uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A5C7H0P01.9e-1232.86Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1[more]
A0A6J1DNV93.2e-1232.62uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DL647.9e-1141.05uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_022156777.14.1e-1435.42uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_022143535.11.2e-1334.53uncharacterized protein LOC111013412 [Momordica charantia][more]
TXG50387.13.9e-1232.86hypothetical protein EZV62_022911 [Acer yangbiense][more]
XP_022155262.16.6e-1232.62uncharacterized protein LOC111022403 [Momordica charantia][more]
KAG6599977.11.2e-1037.17hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT4G29090.17.8e-1135.62Ribonuclease H-like superfamily protein [more]
AT2G34320.11.4e-0731.91Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G65005.19.2e-0422.46Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 23..148
e-value: 2.7E-21
score: 75.7
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 17..150
e-value: 6.5E-13
score: 50.9
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 9..148
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 9..148
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 23..146
e-value: 2.92207E-18
score: 73.8876
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 18..151

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy11g019460.1Lcy11g019460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity