Lcy11g015060 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy11g015060
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Reverse transcriptase domain-containing protein
Location	Chr11: 32988837 .. 32989349 (+)
RNA-Seq Expression	Lcy11g015060
Synteny	Lcy11g015060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTCAGATGTAGCTTGGTTCGAGGAATCGAGTCGCGGAGGAGTTGCTTGGGTCATTCGTGACTCCGATGGATCCTCGATCTGTTTCGGGATGAAGCATATCAAAAGAAGATGGGATATCAATTCGCTTGAAGCGCTTGCTATTTGGGAAGGGTTAAAATGCCTTTCTTACTTCAGGAAGGAGGAGCACTTACCTCTGATTATCGAGTCGGACGCCTTAGAGGTCGTCAGCTGCCTCAATGATCAATCTCAATTTCTCTCGAAGACGAAGAACATCATCGATGCTATTCGCTCCATCTGCTCCGATTGGGGCTCTGTGGAAGTTGTGCACTGCCCTAGGGTAGAAAATCGAGTCGCCCACTCCATTGCTCGCCTTGTTGCTGAGCTTTGTTTTGATTCGCCTGTGGATTTTTCCCAATCTTGGGAAGATGAGTTGCTTTTTTGGTTGTCTAGCTTTCCCAATTGGTTTCTTTCCCTCCTTTATGAGGCTAGTTGTAACCGTTCTCTTTGA

mRNA sequence

ATGAACTCAGATGTAGCTTGGTTCGAGGAATCGAGTCGCGGAGGAGTTGCTTGGGTCATTCGTGACTCCGATGGATCCTCGATCTGTTTCGGGATGAAGCATATCAAAAGAAGATGGGATATCAATTCGCTTGAAGCGCTTGCTATTTGGGAAGGGTTAAAATGCCTTTCTTACTTCAGGAAGGAGGAGCACTTACCTCTGATTATCGAGTCGGACGCCTTAGAGGTCGTCAGCTGCCTCAATGATCAATCTCAATTTCTCTCGAAGACGAAGAACATCATCGATGCTATTCGCTCCATCTGCTCCGATTGGGGCTCTGTGGAAGTTGTGCACTGCCCTAGGGTAGAAAATCGAGTCGCCCACTCCATTGCTCGCCTTGTTGCTGAGCTTTGTTTTGATTCGCCTGTGGATTTTTCCCAATCTTGGGAAGATGAGTTGCTTTTTTGGTTGTCTAGCTTTCCCAATTGGTTTCTTTCCCTCCTTTATGAGGCTAGTTGTAACCGTTCTCTTTGA

Coding sequence (CDS)

ATGAACTCAGATGTAGCTTGGTTCGAGGAATCGAGTCGCGGAGGAGTTGCTTGGGTCATTCGTGACTCCGATGGATCCTCGATCTGTTTCGGGATGAAGCATATCAAAAGAAGATGGGATATCAATTCGCTTGAAGCGCTTGCTATTTGGGAAGGGTTAAAATGCCTTTCTTACTTCAGGAAGGAGGAGCACTTACCTCTGATTATCGAGTCGGACGCCTTAGAGGTCGTCAGCTGCCTCAATGATCAATCTCAATTTCTCTCGAAGACGAAGAACATCATCGATGCTATTCGCTCCATCTGCTCCGATTGGGGCTCTGTGGAAGTTGTGCACTGCCCTAGGGTAGAAAATCGAGTCGCCCACTCCATTGCTCGCCTTGTTGCTGAGCTTTGTTTTGATTCGCCTGTGGATTTTTCCCAATCTTGGGAAGATGAGTTGCTTTTTTGGTTGTCTAGCTTTCCCAATTGGTTTCTTTCCCTCCTTTATGAGGCTAGTTGTAACCGTTCTCTTTGA

Protein sequence

MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFRKEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVAHSIARLVAELCFDSPVDFSQSWEDELLFWLSSFPNWFLSLLYEASCNRSL

Homology

[Close]

BLAST of Lcy11g015060 vs. ExPASy TrEMBL
Match: A0A6P5S2T0 (uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.0e-12
Identity = 46/129 (35.66%), Postives = 70/129 (54.26%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N DVAWF +  RGGV WVIRDS G  +C G +   R      +E LAI   L   ++F+
Sbjct: 86  VNCDVAWFAQLLRGGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHALSACAHFQ 145

Query: 61  KEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVA 120
                 +++ESD    +S LN Q+   S  + I+  I+ + + +  V  V  P+  NRVA
Sbjct: 146 LTH---VMVESDTQRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAPKSCNRVA 205

Query: 121 HSIARLVAE 130
           H++A  V++
Sbjct: 206 HTVAGFVSK 211

[Close]

BLAST of Lcy11g015060 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.6e-12
Identity = 51/161 (31.68%), Postives = 83/161 (51.55%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N+D +W + + RGG+ W+IR  DG  +  G + ++   ++  LEA AI EGL+ L+   
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 61  KEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVA 120
                PL IE+D+ EV S LN + + L+KT  +++ I ++      +      R  N  A
Sbjct: 141 VLR--PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCA 200

Query: 121 HSIARLVAELCFDSPVDFSQSWEDELLFWLSSFPNWFLSLL 162
           HS+A+  + L              E + W+  FPNW LS+L
Sbjct: 201 HSLAQRASVL-------------RESMIWVDCFPNW-LSIL 225

[Close]

BLAST of Lcy11g015060 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 4.4e-12
Identity = 42/129 (32.56%), Postives = 72/129 (55.81%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N++ AW  +++ GG+ W++RD  G  I    + I+   +I  LE +AI EGL+ +   R
Sbjct: 89  LNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAI---R 148

Query: 61  KEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVA 120
           +E   P+ +ESD+LE +  L+ Q Q  ++   +++ I  +  D   V + H  R  N+VA
Sbjct: 149 QEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVA 208

Query: 121 HSIARLVAE 130
           H +AR   E
Sbjct: 209 HGLARRAME 214

[Close]

BLAST of Lcy11g015060 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.3e-11
Identity = 42/134 (31.34%), Postives = 72/134 (53.73%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSY-- 60
           +N+D AW  +++ GG+ W++RD  G  I    + I+   +I  LE +AI EGL+ +    
Sbjct: 89  LNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEH 148

Query: 61  ---FRKEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRV 120
               ++E   P+ +ESD+LE +  L+ Q Q  ++   +++ I  +  D   V + H  R 
Sbjct: 149 CRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISRE 208

Query: 121 ENRVAHSIARLVAE 130
            N+VAH +AR   E
Sbjct: 209 ANKVAHDLARRAME 222

[Close]

BLAST of Lcy11g015060 vs. ExPASy TrEMBL
Match: A0A6P9ERQ1 (uncharacterized protein LOC118348595 OS=Juglans regia OX=51240 GN=LOC118348595 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.6e-09
Identity = 53/155 (34.19%), Postives = 72/155 (46.45%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N D A F E  R GV  V+RD  G  +    K      D   +E LA+  GL+     R
Sbjct: 109 LNIDGATFHEQCRSGVGMVLRDERGKVLFTASKPEHDVSDPIEIEFLAVLRGLQICFPLR 168

Query: 61  KEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVA 120
             E   L +ESD+L VV  LN + + +S   N++  I+ +   +  V V H  R+ N VA
Sbjct: 169 ISE---LEVESDSLLVVQELNKEQESMSVWGNLVSEIKRMLHRFPKVIVQHKGRMANAVA 228

Query: 121 HSIARLVAELCFDSPVDFSQSW-EDELLFWLSSFP 155
           H +AR              QSW  DEL+ W  SFP
Sbjct: 229 HCLAR--------------QSWCIDELVIWWDSFP 246

[Close]

BLAST of Lcy11g015060 vs. NCBI nr
Match: XP_021808158.1 (uncharacterized protein LOC110751913 [Prunus avium])

HSP 1 Score: 82.4 bits (202), Expect = 4.1e-12
Identity = 46/129 (35.66%), Postives = 70/129 (54.26%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N DVAWF +  RGGV WVIRDS G  +C G +   R      +E LAI   L   ++F+
Sbjct: 86  VNCDVAWFAQLLRGGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHALSACAHFQ 145

Query: 61  KEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVA 120
                 +++ESD    +S LN Q+   S  + I+  I+ + + +  V  V  P+  NRVA
Sbjct: 146 LTH---VMVESDTQRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAPKSCNRVA 205

Query: 121 HSIARLVAE 130
           H++A  V++
Sbjct: 206 HTVAGFVSK 211

[Close]

BLAST of Lcy11g015060 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 82.0 bits (201), Expect = 5.3e-12
Identity = 51/161 (31.68%), Postives = 83/161 (51.55%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N+D +W + + RGG+ W+IR  DG  +  G + ++   ++  LEA AI EGL+ L+   
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 61  KEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVA 120
                PL IE+D+ EV S LN + + L+KT  +++ I ++      +      R  N  A
Sbjct: 141 VLR--PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCA 200

Query: 121 HSIARLVAELCFDSPVDFSQSWEDELLFWLSSFPNWFLSLL 162
           HS+A+  + L              E + W+  FPNW LS+L
Sbjct: 201 HSLAQRASVL-------------RESMIWVDCFPNW-LSIL 225

[Close]

BLAST of Lcy11g015060 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 81.3 bits (199), Expect = 9.1e-12
Identity = 42/129 (32.56%), Postives = 72/129 (55.81%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N++ AW  +++ GG+ W++RD  G  I    + I+   +I  LE +AI EGL+ +   R
Sbjct: 89  LNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAI---R 148

Query: 61  KEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVA 120
           +E   P+ +ESD+LE +  L+ Q Q  ++   +++ I  +  D   V + H  R  N+VA
Sbjct: 149 QEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVA 208

Query: 121 HSIARLVAE 130
           H +AR   E
Sbjct: 209 HGLARRAME 214

[Close]

BLAST of Lcy11g015060 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-11
Identity = 42/134 (31.34%), Postives = 72/134 (53.73%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSY-- 60
           +N+D AW  +++ GG+ W++RD  G  I    + I+   +I  LE +AI EGL+ +    
Sbjct: 89  LNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEH 148

Query: 61  ---FRKEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRV 120
               ++E   P+ +ESD+LE +  L+ Q Q  ++   +++ I  +  D   V + H  R 
Sbjct: 149 CRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISRE 208

Query: 121 ENRVAHSIARLVAE 130
            N+VAH +AR   E
Sbjct: 209 ANKVAHDLARRAME 222

[Close]

BLAST of Lcy11g015060 vs. NCBI nr
Match: XP_037439691.1 (uncharacterized protein LOC119307722 isoform X1 [Triticum dicoccoides])

HSP 1 Score: 78.6 bits (192), Expect = 5.9e-11
Identity = 43/128 (33.59%), Postives = 65/128 (50.78%), Query Frame = 0


Query: 1   MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFR 60
           +N D ++  +   G    V+RDS G+ I       +   D+ S+EALA+ EGL      R
Sbjct: 310 LNVDASYHVDEGAGATGAVLRDSSGAFIAASCSFRQYAMDVPSMEALALLEGL------R 369

Query: 61  KEEHL---PLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVEN 120
             EHL    L++ESD+ E+V  + D S++ +    + D  R + S +     VHC R  N
Sbjct: 370 LAEHLGIYALVVESDSQEIVQAMTDPSEYRASAAVVTDDCRQVLSSFSRATTVHCARESN 429

Query: 121 RVAHSIAR 126
            VAH +AR
Sbjct: 430 GVAHWLAR 431

[Close]

BLAST of Lcy11g015060 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 2.2e-08
Identity = 44/160 (27.50%), Postives = 71/160 (44.38%), Query Frame = 0


Query: 2   NSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFRK 61
           N+D  W  E+ R G+ W++R+  G  +  G + + R  ++   E  A+   +  +S F  
Sbjct: 148 NTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSRFNY 207

Query: 62  EEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVAH 121
           +    +I ESDA  +V+ LN    F    +  ++ I+ +   +  V+    PR  N+VA 
Sbjct: 208 KR---IIFESDAQALVNLLN-SDDFWPTLQPALEDIQQLLHHFEEVKFEFTPRGGNKVAD 267

Query: 122 SIARLVAELCFDSPVDFSQSWEDELLFWLSSFPNWFLSLL 162
            IAR          + FS    D  LF  S  P W  S L
Sbjct: 268 RIAR--------ESISFSN--YDPKLF--SIVPQWLRSTL 291

[Close]

BLAST of Lcy11g015060 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 4.2e-07
Identity = 32/124 (25.81%), Postives = 59/124 (47.58%), Query Frame = 0


Query: 2   NSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFRK 61
           N+D  W  ++ R G+ WV+R+  G     G + + +   +   E  A+   +  LS F  
Sbjct: 431 NTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRF-- 490

Query: 62  EEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVAH 121
            ++  +I ESD+  ++  LN+  +     K  I  ++ + S +  V+ V  PR  N +A 
Sbjct: 491 -QYNYVIFESDSQVLIEILNN-DEIWPSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLAE 550

Query: 122 SIAR 126
            +AR
Sbjct: 551 RVAR 550

The following BLAST results are available for this feature:

BLAST of Lcy11g015060 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy11g015060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6P5S2T0	2.0e-12	35.66	uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE...	[more]
A0A6J1DNV9	2.6e-12	31.68	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1CP26	4.4e-12	32.56	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	1.3e-11	31.34	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6P9ERQ1	4.6e-09	34.19	uncharacterized protein LOC118348595 OS=Juglans regia OX=51240 GN=LOC118348595 P...	[more]

BLAST of Lcy11g015060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_021808158.1	4.1e-12	35.66	uncharacterized protein LOC110751913 [Prunus avium]	[more]
XP_022155262.1	5.3e-12	31.68	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022143535.1	9.1e-12	32.56	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	2.7e-11	31.34	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_037439691.1	5.9e-11	33.59	uncharacterized protein LOC119307722 isoform X1 [Triticum dicoccoides]	[more]

BLAST of Lcy11g015060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	2.2e-08	27.50	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	4.2e-07	25.81	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 2..125 e-value: 1.8E-21 score: 76.2
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..131 e-value: 3.8E-15 score: 58.0
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 1..137
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 1..137
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 2..125 e-value: 7.41711E-25 score: 91.2216
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 2..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy11g015060.1	Lcy11g015060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity