Lcy11g014650 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy11g014650
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily
Location	Chr11: 30584449 .. 30586318 (-)
RNA-Seq Expression	Lcy11g014650
Synteny	Lcy11g014650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTACTCACCTACGTGGTTATGTTTTCCATACTATTTTCATACGCCAACTTCGTATGTAGCATTGTTTTTAATGCTACAGTTTCTCAAGATGGAACGGGAGACTTTCGAAGTATTGGTGCTGCTATTGCAGCAGCACCAAATAATAGCAACTCTAGGTTTTACATTCATGTAAAACCCGGAATCTACAAAGAACACGTGGAGGTTGAAAGTCAAAAGACTTTCATCGCCTTAATAGGAGAGGATGCCGCCAAAACAGTCATTGTGGATAACAGGAGCAATGGCACGGGTTTTGGAACCATCGAATCCTCCACATTCGGTAAGTATGAGCAACCCAACCTTTTCTTTTGTCTTCTCTTTTGAATTCGTTCATTTAAAAAATATTAATTATTGGTCTATGAGCCGAATGAAAAATCCTCTTTCTTTCTGTTGATTTTTTAATTTTTAGTTTGTTCCAATCGACACTGTTGATGGTCGATTTTGGTCAGGGGAAACTCACCTGACCTTCACGTGCCACCAACGGTAAATTTGTAGATGAGGGGGAAGATGTGGCCTGCGAAAGAGAAAACCTGCACACCGGTGTGGTGCTAGCCACACCGCCTCCGATGCTTAAGTCAGAAAACGGAAGGGAGAGCTAGAGAGCAAGGGAAAGAGAGTCGAGAATCGAGTTCGGGTCCCCTTCTTCAGTGGCGAAGAGGGGTATAAATACCTGCTCGTGCTCCTAGGGTTTTTAGGAATCCGGAGGCGTTTCGGGAAAAACCAGGCGAAATCGGGGCGGCTAGAGGCATCAGGGACCGAGAGGAGGTGACCGAGCTCGGCCCGCGCAAGCGGGCCGAATGGTCGGCCTCGGCCTTGGGCCGAGGCCGACCATATGGGTCGGTTTTTCCTCGGGCTTGGTCTCCAAGCCCGATTTTCGCCCGGACGTCCTCGTCAGCTCCTTGTGCGTCGGGTGGTCCAAAATTGCCTATAACAGACACCATAAGTTAACTTCATTAATTATTAGAACCTTCACTATTTTACTCTGTTCTCTGTTTCTTCAATTTTTGGCTTAATTGTCTAGAAATTTTCATGGGTTACCCAAATGTTTTTCTCTCTGTGCAGAATTAATATTTAGAATTTCTTTTGTTTCCTTAAATATATATATACTAATTATTGTATTGTACTTTTTGACATTTTGTTTGCAGTTGTGAGCGATAACAACTTTATGGCACAGTCTATAACATTTGAAAATTCTGCTGGACCTAGAAATGGCCAAGCAGTTGCCCTTTTCAATGTTGCCAATTTTACTGTATACTATGAGTGCATATTTCTCGGTTTTCAAGACACGCTTTTTGTAAATAGAATGAAATCATTCTTTAAAGGTTGTGATATCTACGGAACGATAGATTTTATATTTGGGGATGGACTGGCAGTGTTTCAGGATTGCAAAATATATGCAAGATTACCCAACAATCTTGTTACCATCACTGCAGAGTCCAAAGAGTCCCAGAATGAACAAAGTGGCTTCGTCTTCCAAAATTTCAGTGTAATGGCCTCTCCTGAACTTGGGTCAAAAGAAGTAAATAGCTCTCAAGTTTATCTTGGTCGGCCATGGAGAGAGTATTCAACGGTTATTTTTATGCAATCATTTTTAGACCATGTGGTCAATCCAGCAGGATGGTTAGCTTGGTCGACCCCTCTAAATTTTTTATTCTATGCAGAATTTAATAATAGAGGCCCAGAAGCTGATTTAAGTAGAAGAGTCAAGTGGCCAGGATTTCATGTGCTAAACAATCTAGTAGAAGCCACTAATTTTACCGTTAGAATTTTCATTGATGGAAACACATGGTTGCCCGAGACGGGTGTGCCATATAGGTCAAGCTTATAG

mRNA sequence

ATGGCCTTACTCACCTACGTGGTTATGTTTTCCATACTATTTTCATACGCCAACTTCGTATGTAGCATTGTTTTTAATGCTACAGTTTCTCAAGATGGAACGGGAGACTTTCGAAGTATTGGTGCTGCTATTGCAGCAGCACCAAATAATAGCAACTCTAGGTTTTACATTCATGTAAAACCCGGAATCTACAAAGAACACGTGGAGGTTGAAAGTCAAAAGACTTTCATCGCCTTAATAGGAGAGGATGCCGCCAAAACAGTCATTGTGGATAACAGGAGCAATGGCACGGGTTTTGGAACCATCGAATCCTCCACATTCGTTGTGAGCGATAACAACTTTATGGCACAGTCTATAACATTTGAAAATTCTGCTGGACCTAGAAATGGCCAAGCAGTTGCCCTTTTCAATGTTGCCAATTTTACTGTATACTATGAGTGCATATTTCTCGGTTTTCAAGACACGCTTTTTGTAAATAGAATGAAATCATTCTTTAAAGGTTGTGATATCTACGGAACGATAGATTTTATATTTGGGGATGGACTGGCAGTGTTTCAGGATTGCAAAATATATGCAAGATTACCCAACAATCTTGTTACCATCACTGCAGAGTCCAAAGAGTCCCAGAATGAACAAAGTGGCTTCGTCTTCCAAAATTTCAGTGTAATGGCCTCTCCTGAACTTGGGTCAAAAGAAGTAAATAGCTCTCAAGTTTATCTTGGTCGGCCATGGAGAGAGTATTCAACGGTTATTTTTATGCAATCATTTTTAGACCATGTGGTCAATCCAGCAGGATGGTTAGCTTGGTCGACCCCTCTAAATTTTTTATTCTATGCAGAATTTAATAATAGAGGCCCAGAAGCTGATTTAAGTAGAAGAGTCAAGTGGCCAGGATTTCATGTGCTAAACAATCTAGTAGAAGCCACTAATTTTACCGTTAGAATTTTCATTGATGGAAACACATGGTTGCCCGAGACGGGTGTGCCATATAGGTCAAGCTTATAG

Coding sequence (CDS)

ATGGCCTTACTCACCTACGTGGTTATGTTTTCCATACTATTTTCATACGCCAACTTCGTATGTAGCATTGTTTTTAATGCTACAGTTTCTCAAGATGGAACGGGAGACTTTCGAAGTATTGGTGCTGCTATTGCAGCAGCACCAAATAATAGCAACTCTAGGTTTTACATTCATGTAAAACCCGGAATCTACAAAGAACACGTGGAGGTTGAAAGTCAAAAGACTTTCATCGCCTTAATAGGAGAGGATGCCGCCAAAACAGTCATTGTGGATAACAGGAGCAATGGCACGGGTTTTGGAACCATCGAATCCTCCACATTCGTTGTGAGCGATAACAACTTTATGGCACAGTCTATAACATTTGAAAATTCTGCTGGACCTAGAAATGGCCAAGCAGTTGCCCTTTTCAATGTTGCCAATTTTACTGTATACTATGAGTGCATATTTCTCGGTTTTCAAGACACGCTTTTTGTAAATAGAATGAAATCATTCTTTAAAGGTTGTGATATCTACGGAACGATAGATTTTATATTTGGGGATGGACTGGCAGTGTTTCAGGATTGCAAAATATATGCAAGATTACCCAACAATCTTGTTACCATCACTGCAGAGTCCAAAGAGTCCCAGAATGAACAAAGTGGCTTCGTCTTCCAAAATTTCAGTGTAATGGCCTCTCCTGAACTTGGGTCAAAAGAAGTAAATAGCTCTCAAGTTTATCTTGGTCGGCCATGGAGAGAGTATTCAACGGTTATTTTTATGCAATCATTTTTAGACCATGTGGTCAATCCAGCAGGATGGTTAGCTTGGTCGACCCCTCTAAATTTTTTATTCTATGCAGAATTTAATAATAGAGGCCCAGAAGCTGATTTAAGTAGAAGAGTCAAGTGGCCAGGATTTCATGTGCTAAACAATCTAGTAGAAGCCACTAATTTTACCGTTAGAATTTTCATTGATGGAAACACATGGTTGCCCGAGACGGGTGTGCCATATAGGTCAAGCTTATAG

Protein sequence

MALLTYVVMFSILFSYANFVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVNPAGWLAWSTPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGNTWLPETGVPYRSSL

Homology

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BLAST of Lcy11g014650 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.5e-80
Identity = 147/315 (46.67%), Postives = 205/315 (65.08%), Query Frame = 0


Query: 24  VFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGED 83
           V +A V+ DGTG+F +I  A+ AAP+ S  R+ IHVK G+Y E+VE++ +K  I ++G+ 
Sbjct: 212 VADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDG 271

Query: 84  AAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTV 143
              TVI  NRS   G+ T  S+TF VS   F+A+ ITF+N+AGP   QAVA+ +  +  V
Sbjct: 272 IDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGV 331

Query: 144 YYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYAR--LPNNLVTI 203
           +Y C   G+QDTL+ + M+ FF+ C I GT+DFIFGD  AVFQ C+I A+  LPN   +I
Sbjct: 332 FYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSI 391

Query: 204 TAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVV 263
           TA+ ++  NE +GF  Q  ++ A  +L    +N++  YLGRPW+ YS  +FMQ+++   +
Sbjct: 392 TAQGRKDPNEPTGFTIQFSNIAADTDL-LLNLNTTATYLGRPWKLYSRTVFMQNYMSDAI 451

Query: 264 NPAGWLAW--STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDG 323
           NP GWL W  +  L+ L+Y E+ N GP A L RRVKWPG+HVLN   EA NFTV   I G
Sbjct: 452 NPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQG 511

Query: 324 NTWLPETGVPYRSSL 335
           N WLP TG+ + + L
Sbjct: 512 NLWLPSTGITFIAGL 525

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BLAST of Lcy11g014650 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.2e-80
Identity = 146/309 (47.25%), Postives = 204/309 (66.02%), Query Frame = 0


Query: 26  NATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDAA 85
           +A V+QDGTG+F ++  A+ AAP+ S  R+ I++K G YKE+VE++ +K  + +IG+   
Sbjct: 208 DAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMD 267

Query: 86  KTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVYY 145
            T+I  NRS   G+ T  S+TF VS   F+A+ ITFEN+AGP   QAVAL + ++ +V+Y
Sbjct: 268 ATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFY 327

Query: 146 ECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYAR--LPNNLVTITA 205
            C   G+QDTL+ + M+ F++ C I GT+DFIFGD   VFQ+C+I A+  LPN   +ITA
Sbjct: 328 RCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITA 387

Query: 206 ESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVNP 265
           + ++  NE +G   Q  ++ A  +L +  VNS+  YLGRPW+ YS  + MQSFL +V+ P
Sbjct: 388 QGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRP 447

Query: 266 AGWLAWS--TPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGNT 325
            GWL W+    LN LFY E+ N GP A L  RVKWPG+ V N   +A N+TV  FI+GN 
Sbjct: 448 EGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNL 507

Query: 326 WLPETGVPY 331
           WLP TGV Y
Sbjct: 508 WLPSTGVKY 516

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BLAST of Lcy11g014650 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 292.7 bits (748), Expect = 5.1e-78
Identity = 145/314 (46.18%), Postives = 208/314 (66.24%), Query Frame = 0


Query: 25  FNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDA 84
           ++ +V+ DGTG+F  I  AI  AP+ S++RF I++K G+Y E+VE++ +K  I ++G+  
Sbjct: 211 YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGI 270

Query: 85  AKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVY 144
             TVI  NRS   G+ T  S+TF VS   F+A+ ITF+N+AGP   QAVAL + ++ +V+
Sbjct: 271 DVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSVF 330

Query: 145 YECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYAR--LPNNLVTIT 204
           + C   G+QDTL+ + M+ F++ C I GT+DFIFGDG  VFQ+C+I A+  LPN   TIT
Sbjct: 331 FRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTIT 390

Query: 205 AESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVN 264
           A+ ++  N+ SGF  Q  ++ A  +L    +N+++ YLGRPW+ YS  +F+++ +  VV 
Sbjct: 391 AQGRKDVNQPSGFSIQFSNISADADL-VPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVR 450

Query: 265 PAGWLAWST--PLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGN 324
           P GWL W+    L+ LFY EF N GP + LS RVKWPG+HV NN  +A NFTV  FI GN
Sbjct: 451 PEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGN 510

Query: 325 TWLPETGVPYRSSL 335
            WLP TGV +   L
Sbjct: 511 LWLPSTGVTFSDGL 523

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BLAST of Lcy11g014650 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.3e-77
Identity = 141/315 (44.76%), Postives = 212/315 (67.30%), Query Frame = 0


Query: 28  TVSQDGTGDFRSIGAAIAAAPNNSNSR---FYIHVKPGIYKEHVEVESQKTFIALIGEDA 87
           TVSQDGTG+F +I AA+AAAPNN++     F I+V  GIY+E++ +   K ++ +IG+  
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 88  AKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVY 147
            +TV+  NRS   G+ T  S+TF V+  NF+A +ITF N+AGP   QAVAL + A+F+++
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 148 YECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYAR--LPNNLVTIT 207
           Y C F  +QDTL+ + ++ F++ CD+YGT+DFIFG+   VFQ+C +Y R  +PN    IT
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 208 AESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVN 267
           A+ +   N+ +G   QN ++  + +L S    + + YLGRPW+EYS  ++MQS++D  V 
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNY-TVKTYLGRPWKEYSRTVYMQSYIDGFVE 505

Query: 268 PAGWLAWS--TPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVR-IFIDG 327
           P GW  W+    L+ L+YAE+NN GP ++ + RV WPG+HV+N+  +A NFTV  +FI+ 
Sbjct: 506 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINS-TDAANFTVTGLFIEA 565

Query: 328 NTWLPETGVPYRSSL 335
           + W+ +TGVPY S L
Sbjct: 566 D-WIWKTGVPYTSGL 577

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BLAST of Lcy11g014650 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.7e-76
Identity = 139/313 (44.41%), Postives = 208/313 (66.45%), Query Frame = 0


Query: 26  NATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDAA 85
           N  V+QDG+G+ ++I  A+AAA     SR+ I++K G Y E++EV+ +   I  +G+   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 86  KTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVYY 145
           KT+I  ++S G G  T +S+T  V  +NF+A+ IT  N+AGP N QAVAL + ++ +V+Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 146 ECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYARL-PNNLVTITAE 205
            C F G+QDTL+V+  + F++ CDIYGT+DFIFG+   V Q+C I+AR  PN   T+TA+
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 206 SKESQNEQSGFVFQNFSVMASPELGSKEVNSS-QVYLGRPWREYSTVIFMQSFLDHVVNP 265
            +   N+ +G +  N  V A+ +L  K V SS + +LGRPW++YS  +++++FLD ++NP
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDL--KPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINP 441

Query: 266 AGWLAWS--TPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGNT 325
           AGW+ WS    LN L+YAE+ N GP +  + RVKW G+HVL +  + + FTV  FI GN+
Sbjct: 442 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 501

Query: 326 WLPETGVPYRSSL 335
           WLP T VP+ S L
Sbjct: 502 WLPATNVPFTSGL 510

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BLAST of Lcy11g014650 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 7.4e-112
Identity = 196/329 (59.57%), Postives = 247/329 (75.08%), Query Frame = 0


Query: 7   VVMFSILFSYANFVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKE 66
           V +  ++  +  F    +FNATVS DG+G+F  +  AIAAAPN S +RFYIHVKPGIYKE
Sbjct: 11  VSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKPGIYKE 70

Query: 67  HVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAG 126
           HVEV  QKTFIALIGEDA+ T+IVDNRSNG+GF T  ++T +V   NFMAQ +TFENSA 
Sbjct: 71  HVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFENSAE 130

Query: 127 PRNGQAVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQ 186
            + GQA+A+ + A  T YY+C+FL +QDTL+   +  FFK CDIYG IDFIFG+GL VFQ
Sbjct: 131 QKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLVVFQ 190

Query: 187 DCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWRE 246
           DC IYAR+P   + +TA+SK +  +QSGF+FQN +V  SPE+ S + N + ++LGRPWR+
Sbjct: 191 DCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVT-MFLGRPWRD 250

Query: 247 YSTVIFMQSFLDHVVNPAGWLAW-STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNL 306
           YS VIFM+SFLD VV P GWL W   P+N LFYAE+NNRGP AD S+RV WPG+HVL+N 
Sbjct: 251 YSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHVLHNA 310

Query: 307 VEATNFTVRIFIDGNTWLPETGVPYRSSL 335
           +E T FTV  FI+G  WLPETG+P+R+ L
Sbjct: 311 MEVTKFTVENFINGTKWLPETGIPFRAGL 338

[Close]

BLAST of Lcy11g014650 vs. ExPASy TrEMBL
Match: A0A6J1D863 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 9.0e-110
Identity = 197/329 (59.88%), Postives = 241/329 (73.25%), Query Frame = 0


Query: 7   VVMFSILFSYANFVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKE 66
           V +  IL  +  F  S  FN TVS DG G+F  +  AIAAAPN S SRFYIHVKPG Y E
Sbjct: 11  VTLLVILDHFVEFSYSFTFNITVSLDGMGNFFRVNDAIAAAPNFSTSRFYIHVKPGTYNE 70

Query: 67  HVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAG 126
           HVEV +QKTFIALIG+DA+ T+IVDNRSNGTGF T  S+T  VS  NFMAQS+TF+NSAG
Sbjct: 71  HVEVPAQKTFIALIGDDASTTIIVDNRSNGTGFSTATSATLTVSGENFMAQSLTFQNSAG 130

Query: 127 PRNGQAVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQ 186
           P  GQAVA+ + A  TVYY+C+FLG+QDTLF   +  FFK CDIYG++DFIFG+GLA+FQ
Sbjct: 131 PDKGQAVAVLDQAKHTVYYKCVFLGYQDTLFAGAVPQFFKECDIYGSVDFIFGNGLAIFQ 190

Query: 187 DCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWRE 246
           DC IYARL +  +T+TA+SK++ +  SGF+FQ  +V  SPE+ S + N   V+LGRPWR 
Sbjct: 191 DCNIYARLSDIHITVTAQSKDNVDNHSGFIFQACNVTVSPEIVSSK-NKVTVFLGRPWRT 250

Query: 247 YSTVIFMQSFLDHVVNPAGWLAW-STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNL 306
           YS V F++SFLD VV P GWL W  TP+N LFYAE+ N G  ++ S RV WPGFH+ +  
Sbjct: 251 YSMVAFIESFLDDVVQPKGWLEWPGTPVNLLFYAEYKNSGTGSETSNRVDWPGFHLFSYA 310

Query: 307 VEATNFTVRIFIDGNTWLPETGVPYRSSL 335
            E  NFTV  FIDG  WLPETG+P+R+ L
Sbjct: 311 AEVANFTVENFIDGTQWLPETGIPFRTGL 338

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BLAST of Lcy11g014650 vs. ExPASy TrEMBL
Match: A0A6J1D381 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.7e-108
Identity = 198/332 (59.64%), Postives = 249/332 (75.00%), Query Frame = 0


Query: 4   LTYVVMFSILFSYANFVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGI 63
           L ++++ S+ FS+    C I FNATVS DG+GD+  I  AIAAAPNN + RFYIHVKPGI
Sbjct: 12  LVFLLLHSVEFSH----CLITFNATVSLDGSGDYVHISDAIAAAPNNGSIRFYIHVKPGI 71

Query: 64  YKEHVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFEN 123
           YKEH+EV   KTFI+LIG+DA+ TVIVD+RSN  GF T  S+TF V   +F+AQ +TF+N
Sbjct: 72  YKEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTFQN 131

Query: 124 SAGPRNGQAVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLA 183
           SAGP NGQAVA+ + AN+T YY+C+F+G+QDTL+V      FK CD+YG+IDFIFGDGLA
Sbjct: 132 SAGPENGQAVAVLSEANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDGLA 191

Query: 184 VFQDCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRP 243
           +FQDC +YAR+     TITA+SK  ++++SGF FQN +V  SPE+ S + +   V LGRP
Sbjct: 192 IFQDCNVYARILG--TTITAQSKSYKDQRSGFTFQNCNVTVSPEIQSIK-DQVTVSLGRP 251

Query: 244 WREYSTVIFMQSFLDHVVNPAGWLAW-STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVL 303
           WR YSTVIFM+SFLD +V P GW  W  TP+  LFYAE+NN G  AD S+RVKWPG+HVL
Sbjct: 252 WRNYSTVIFMESFLDDIVEPGGWSEWPRTPVYLLFYAEYNNSGSGADTSQRVKWPGYHVL 311

Query: 304 NNLVEATNFTVRIFIDGNTWLPETGVPYRSSL 335
           +N  EA+ FTV IFIDG  WL ETG+P+R  L
Sbjct: 312 HNEDEASRFTVEIFIDGLEWLSETGIPFRRGL 336

[Close]

BLAST of Lcy11g014650 vs. ExPASy TrEMBL
Match: A0A6J1GM54 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 3.8e-108
Identity = 199/327 (60.86%), Postives = 248/327 (75.84%), Query Frame = 0


Query: 12  ILFSYAN---FVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHV 71
           + F +AN    V SI FNA VS DG+GDF+SI  AIAAAPNNSN+RFYI V PGIY+ H+
Sbjct: 10  LFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRVTPGIYRGHL 69

Query: 72  EVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPR 131
           E+   K FIALIG+ A  T+IVDNRS+GTGF T ES+T VV+ +NFMAQ ++FENSA P+
Sbjct: 70  EIPPAKKFIALIGDSALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQHLSFENSAEPQ 129

Query: 132 NGQAVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDC 191
           NGQAVA+ + A  +VYY+C F+ FQDTL+      FF+ CDIYGT+DFIFG+GLAVFQDC
Sbjct: 130 NGQAVAVLDYAKHSVYYDCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIFGNGLAVFQDC 189

Query: 192 KIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYS 251
            IYARLP + +T+TA+SKE    QSGF FQN +V  SPE+ + +    ++YLGRPWREYS
Sbjct: 190 NIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANK-TKVKIYLGRPWREYS 249

Query: 252 TVIFMQSFLDHVVNPAGWLAW-STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVE 311
           TV+FM+SFLD  V P GW++W   P+N LFYAEFNNRGP A+ + RV WP FHVL+ L+ 
Sbjct: 250 TVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWPTFHVLDKLL- 309

Query: 312 ATNFTVRIFIDGNTWLPETGVPYRSSL 335
           A NFTV  FI+G+ WLPET VP+R+ L
Sbjct: 310 AKNFTVENFINGSDWLPETHVPFRTGL 334

[Close]

BLAST of Lcy11g014650 vs. ExPASy TrEMBL
Match: A0A6J1KPG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 3.5e-106
Identity = 187/324 (57.72%), Postives = 241/324 (74.38%), Query Frame = 0


Query: 12  ILFSYANFVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVE 71
           +L  +  F  +  FN+TVS DGTG+F  I  AI AAPN + +RFYIH+KPGIY EH+E+ 
Sbjct: 14  LLLHFLKFSSTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEIP 73

Query: 72  SQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQ 131
           SQKTFI+LIG++A+ T+IVDNRS  TGF T  S+T  V+  NFMA+ +TF+NSAGP+ GQ
Sbjct: 74  SQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNSAGPKMGQ 133

Query: 132 AVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIY 191
           AVA+ + A  T YY+C+FLG+QDTL+   +  FFK CDIYGT+DFIFG+GL VFQDC IY
Sbjct: 134 AVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIY 193

Query: 192 ARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVI 251
           ARL +   T+TA+SK + +EQSGFVFQN +V  SPE+ S   +   V+LGRPWR YS V 
Sbjct: 194 ARLVDIQSTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSR-DKVLVFLGRPWRAYSMVA 253

Query: 252 FMQSFLDHVVNPAGWLAW-STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATN 311
           F++SFLD+VV P GWL W   P+N L+Y E+NNRG  A+ S+RV WPGFHVL++L +   
Sbjct: 254 FVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVAK 313

Query: 312 FTVRIFIDGNTWLPETGVPYRSSL 335
           FTV  FI+G TWLP+T +P+RS L
Sbjct: 314 FTVENFINGTTWLPQTTIPFRSGL 336

[Close]

BLAST of Lcy11g014650 vs. NCBI nr
Match: KAG6584282.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 430.3 bits (1105), Expect = 1.6e-116
Identity = 219/335 (65.37%), Postives = 260/335 (77.61%), Query Frame = 0


Query: 10  FSILFSYANFVCS----IVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYK 69
           F ILF +  F C+    I +NATVS DG GD+ +IGAAIAAAPNNS+SRF IHVKPG+Y 
Sbjct: 4   FFILF-FVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYF 63

Query: 70  EHVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENS- 129
           E++ +ES KTFI L G+DA+ T IV NRS  TGFGT +S+TFVV  N+F+A SITF NS 
Sbjct: 64  EYIRIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSA 123

Query: 130 -AGPRNGQAVALFNV--ANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDG 189
            AGP +GQAVAL N    N+TV+Y+C+FLG+QDTLF+N    FFK C+IYGT+DFIFG+ 
Sbjct: 124 GAGPNHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNA 183

Query: 190 LAVFQDCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLG 249
           L VFQDC+IYARL NN VT+TA+SKE+Q + SGF FQN SVMASPELG   VN + V+LG
Sbjct: 184 LVVFQDCRIYARLLNNTVTVTAQSKENQLQLSGFSFQNCSVMASPELG--PVNETIVFLG 243

Query: 250 RPWREYSTVIFMQSFLDHVVNPAGWLAW--STPLNFLFYAEFNNRGPEADLSRRVKWPGF 309
           RPWR+YSTVIFM+SFLD VVNPAGW  W  S+ L  LFYAE+ NRGP A+LS RVKWPGF
Sbjct: 244 RPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSGRVKWPGF 303

Query: 310 HVLNNLVEATNFTVRIFIDGNTWLPETGVPYRSSL 335
           H + N  EAT F+VR FI+G TWLPETGVPYR  L
Sbjct: 304 HAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

[Close]

BLAST of Lcy11g014650 vs. NCBI nr
Match: KAG6584281.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429.5 bits (1103), Expect = 2.7e-116
Identity = 218/335 (65.07%), Postives = 261/335 (77.91%), Query Frame = 0


Query: 10  FSILFSYANFVCS----IVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYK 69
           F ILF +  F C+    I +NATVS DG GD+ +IGAAIAAAPNNS+SRF IHVKPG+Y 
Sbjct: 4   FFILF-FVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYF 63

Query: 70  EHVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENS- 129
           E++++ES KTFI L G+DA+ T IV NRS  TGFGT +S+TFVV  N+F+A SITF NS 
Sbjct: 64  EYIKIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSA 123

Query: 130 -AGPRNGQAVALFNV--ANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDG 189
            AGP++GQAVAL N    N+TV+Y+C+FLG+QDTLF+N    FFK C+IYGT+DFIFG+ 
Sbjct: 124 GAGPKHGQAVALLNSNNENYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNA 183

Query: 190 LAVFQDCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLG 249
           L VFQDC+IYARL NN VT+TA+SKE + + SGF FQN SVMASPELG   VN + V+LG
Sbjct: 184 LVVFQDCRIYARLLNNTVTVTAQSKEYKRQLSGFSFQNCSVMASPELG--PVNETIVFLG 243

Query: 250 RPWREYSTVIFMQSFLDHVVNPAGWLAW--STPLNFLFYAEFNNRGPEADLSRRVKWPGF 309
           RPWR+YSTVIFM+SFLD VVNPAGW  W  S+ L  LFYAE+ NRGP A+LS RVKWPGF
Sbjct: 244 RPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSGRVKWPGF 303

Query: 310 HVLNNLVEATNFTVRIFIDGNTWLPETGVPYRSSL 335
           H + N  EAT F+VR FI+G TWLPETGVPYR  L
Sbjct: 304 HAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

[Close]

BLAST of Lcy11g014650 vs. NCBI nr
Match: KAG7019877.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414.1 bits (1063), Expect = 1.2e-111
Identity = 203/307 (66.12%), Postives = 245/307 (79.80%), Query Frame = 0


Query: 34  TGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDAAKTVIVDNR 93
           TGD+ +IGAAIAAAPNNS+SRF IHVKPG+Y E++++ES KTFI L G+DA+ T IV NR
Sbjct: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145

Query: 94  SNGTGFGTIESSTFVVSDNNFMAQSITFENS--AGPRNGQAVALFNV--ANFTVYYECIF 153
           S  TGFGT +S+TFVV  N+F+A SITF NS  AGP++GQAVAL N    N+TV+Y+C+F
Sbjct: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205

Query: 154 LGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYARLPNNLVTITAESKESQ 213
           LG+QDTLF+N    FFK C+IYGT+DFIFG+ L VFQDC+IYARL NN VT+TA+SK++ 
Sbjct: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265

Query: 214 NEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVNPAGWLAW 273
            + SGF FQN SVM SPELG   VN + V+LGRPWR+YSTVIFM+SFLD VVNPAGW  W
Sbjct: 266 KQLSGFSFQNCSVMVSPELG--PVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEW 325

Query: 274 --STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGNTWLPETG 333
             S+ L  LFYAE++NRGP A+LS RVKWPGFH + N  EAT F+VR FI+G TWLPETG
Sbjct: 326 SNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETG 385

Query: 334 VPYRSSL 335
           VPYR  L
Sbjct: 386 VPYRPDL 390

[Close]

BLAST of Lcy11g014650 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 413.7 bits (1062), Expect = 1.5e-111
Identity = 196/329 (59.57%), Postives = 247/329 (75.08%), Query Frame = 0


Query: 7   VVMFSILFSYANFVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKE 66
           V +  ++  +  F    +FNATVS DG+G+F  +  AIAAAPN S +RFYIHVKPGIYKE
Sbjct: 11  VSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKPGIYKE 70

Query: 67  HVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAG 126
           HVEV  QKTFIALIGEDA+ T+IVDNRSNG+GF T  ++T +V   NFMAQ +TFENSA 
Sbjct: 71  HVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFENSAE 130

Query: 127 PRNGQAVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQ 186
            + GQA+A+ + A  T YY+C+FL +QDTL+   +  FFK CDIYG IDFIFG+GL VFQ
Sbjct: 131 QKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLVVFQ 190

Query: 187 DCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWRE 246
           DC IYAR+P   + +TA+SK +  +QSGF+FQN +V  SPE+ S + N + ++LGRPWR+
Sbjct: 191 DCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVT-MFLGRPWRD 250

Query: 247 YSTVIFMQSFLDHVVNPAGWLAW-STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNL 306
           YS VIFM+SFLD VV P GWL W   P+N LFYAE+NNRGP AD S+RV WPG+HVL+N 
Sbjct: 251 YSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHVLHNA 310

Query: 307 VEATNFTVRIFIDGNTWLPETGVPYRSSL 335
           +E T FTV  FI+G  WLPETG+P+R+ L
Sbjct: 311 MEVTKFTVENFINGTKWLPETGIPFRAGL 338

[Close]

BLAST of Lcy11g014650 vs. NCBI nr
Match: XP_023511664.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.1 bits (1045), Expect = 1.4e-109
Identity = 207/338 (61.24%), Postives = 254/338 (75.15%), Query Frame = 0


Query: 1   MALLTYVVMFSILFSYAN---FVCSIVFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYI 60
           MA L  V    + F +AN    V  I FNATVS DG+GDF+SI  AIAAAPNNSN+RFYI
Sbjct: 1   MAFLQLVA--CLFFFFANVLALVDPIRFNATVSLDGSGDFKSISEAIAAAPNNSNTRFYI 60

Query: 61  HVKPGIYKEHVEVESQKTFIALIGEDAAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQ 120
            V PGIY+ H+E+   K FIALIG++A  T+IVDNRS+GTGF T ES+T VV+ +NFMAQ
Sbjct: 61  RVTPGIYRGHLEIPPAKKFIALIGDNALTTIIVDNRSHGTGFKTSESTTLVVNGDNFMAQ 120

Query: 121 SITFENSAGPRNGQAVALFNVANFTVYYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFI 180
            ++FENSA P+NGQAVA+ + A  TVYY+C FL FQDTL+      FF+ CDIYGT+DFI
Sbjct: 121 HLSFENSAEPQNGQAVAVLDYAKHTVYYDCKFLSFQDTLYSKGEFQFFRECDIYGTVDFI 180

Query: 181 FGDGLAVFQDCKIYARLPNNLVTITAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQ 240
           FG+GLAVFQDC IYARLP + +T+TA+SKE    QSGF FQN +V  SPE+ + + N  +
Sbjct: 181 FGNGLAVFQDCNIYARLPMDEITVTAQSKEQSMSQSGFSFQNCTVTVSPEITANKTN-VK 240

Query: 241 VYLGRPWREYSTVIFMQSFLDHVVNPAGWLAW-STPLNFLFYAEFNNRGPEADLSRRVKW 300
           +YLGRPWREYSTV+FM+SFLD  V P GW++W   P+N LFYAEFNNRGP AD + RV W
Sbjct: 241 IYLGRPWREYSTVVFMESFLDDNVQPQGWISWGGVPINNLFYAEFNNRGPGADTTHRVNW 300

Query: 301 PGFHVLNNLVEATNFTVRIFIDGNTWLPETGVPYRSSL 335
           P FHVL+ L+ A NFTV  FI+G+ WLPET VP+R+ L
Sbjct: 301 PTFHVLDKLL-AKNFTVENFINGSDWLPETHVPFRTGL 334

[Close]

BLAST of Lcy11g014650 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 300.4 bits (768), Expect = 1.7e-81
Identity = 147/315 (46.67%), Postives = 205/315 (65.08%), Query Frame = 0


Query: 24  VFNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGED 83
           V +A V+ DGTG+F +I  A+ AAP+ S  R+ IHVK G+Y E+VE++ +K  I ++G+ 
Sbjct: 212 VADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDG 271

Query: 84  AAKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTV 143
              TVI  NRS   G+ T  S+TF VS   F+A+ ITF+N+AGP   QAVA+ +  +  V
Sbjct: 272 IDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGV 331

Query: 144 YYECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYAR--LPNNLVTI 203
           +Y C   G+QDTL+ + M+ FF+ C I GT+DFIFGD  AVFQ C+I A+  LPN   +I
Sbjct: 332 FYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSI 391

Query: 204 TAESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVV 263
           TA+ ++  NE +GF  Q  ++ A  +L    +N++  YLGRPW+ YS  +FMQ+++   +
Sbjct: 392 TAQGRKDPNEPTGFTIQFSNIAADTDL-LLNLNTTATYLGRPWKLYSRTVFMQNYMSDAI 451

Query: 264 NPAGWLAW--STPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDG 323
           NP GWL W  +  L+ L+Y E+ N GP A L RRVKWPG+HVLN   EA NFTV   I G
Sbjct: 452 NPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQG 511

Query: 324 NTWLPETGVPYRSSL 335
           N WLP TG+ + + L
Sbjct: 512 NLWLPSTGITFIAGL 525

[Close]

BLAST of Lcy11g014650 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 292.7 bits (748), Expect = 3.6e-79
Identity = 145/314 (46.18%), Postives = 208/314 (66.24%), Query Frame = 0


Query: 25  FNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDA 84
           ++ +V+ DGTG+F  I  AI  AP+ S++RF I++K G+Y E+VE++ +K  I ++G+  
Sbjct: 211 YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGI 270

Query: 85  AKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVY 144
             TVI  NRS   G+ T  S+TF VS   F+A+ ITF+N+AGP   QAVAL + ++ +V+
Sbjct: 271 DVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSVF 330

Query: 145 YECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYAR--LPNNLVTIT 204
           + C   G+QDTL+ + M+ F++ C I GT+DFIFGDG  VFQ+C+I A+  LPN   TIT
Sbjct: 331 FRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTIT 390

Query: 205 AESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVN 264
           A+ ++  N+ SGF  Q  ++ A  +L    +N+++ YLGRPW+ YS  +F+++ +  VV 
Sbjct: 391 AQGRKDVNQPSGFSIQFSNISADADL-VPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVR 450

Query: 265 PAGWLAWST--PLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGN 324
           P GWL W+    L+ LFY EF N GP + LS RVKWPG+HV NN  +A NFTV  FI GN
Sbjct: 451 PEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGN 510

Query: 325 TWLPETGVPYRSSL 335
            WLP TGV +   L
Sbjct: 511 LWLPSTGVTFSDGL 523

[Close]

BLAST of Lcy11g014650 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 289.7 bits (740), Expect = 3.1e-78
Identity = 141/315 (44.76%), Postives = 212/315 (67.30%), Query Frame = 0


Query: 28  TVSQDGTGDFRSIGAAIAAAPNNSNSR---FYIHVKPGIYKEHVEVESQKTFIALIGEDA 87
           TVSQDGTG+F +I AA+AAAPNN++     F I+V  GIY+E++ +   K ++ +IG+  
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 88  AKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVY 147
            +TV+  NRS   G+ T  S+TF V+  NF+A +ITF N+AGP   QAVAL + A+F+++
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 148 YECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYAR--LPNNLVTIT 207
           Y C F  +QDTL+ + ++ F++ CD+YGT+DFIFG+   VFQ+C +Y R  +PN    IT
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 208 AESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVN 267
           A+ +   N+ +G   QN ++  + +L S    + + YLGRPW+EYS  ++MQS++D  V 
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNY-TVKTYLGRPWKEYSRTVYMQSYIDGFVE 505

Query: 268 PAGWLAWS--TPLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVR-IFIDG 327
           P GW  W+    L+ L+YAE+NN GP ++ + RV WPG+HV+N+  +A NFTV  +FI+ 
Sbjct: 506 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINS-TDAANFTVTGLFIEA 565

Query: 328 NTWLPETGVPYRSSL 335
           + W+ +TGVPY S L
Sbjct: 566 D-WIWKTGVPYTSGL 577

[Close]

BLAST of Lcy11g014650 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 139/310 (44.84%), Postives = 203/310 (65.48%), Query Frame = 0


Query: 25  FNATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDA 84
           +N +V+ DGTG+F +I  A+ AAPN S +RF I++K G Y E+VE+  +KT I  IG+  
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 85  AKTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVY 144
            KTVI  NRS   G+ T ++ T  V    ++A+ I+F NSAGP   QAVA  + ++ + +
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAF 339

Query: 145 YECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYARLPN--NLVTIT 204
           Y C F G+QDTL+V+  K F++ CDIYGTIDFIFG+   VFQ+  +YAR PN  + +  T
Sbjct: 340 YRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFT 399

Query: 205 AESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVN 264
           A+S+   ++ +G    N  ++A+P+L   + N  + YLGRPWR+YS  + ++SF+D +++
Sbjct: 400 AQSRNQSDQPTGISILNCRILAAPDLIPVKEN-FKAYLGRPWRKYSRTVIIKSFIDDLIH 459

Query: 265 PAGWLAWST--PLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGN 324
           PAGWL       L  L+Y E+ N GP A++++RV WPGF  + N  EAT FTV  FIDG+
Sbjct: 460 PAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGS 519

Query: 325 TWLPETGVPY 331
           TWL  TG+P+
Sbjct: 520 TWLNSTGIPF 528

[Close]

BLAST of Lcy11g014650 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 136/313 (43.45%), Postives = 203/313 (64.86%), Query Frame = 0


Query: 26  NATVSQDGTGDFRSIGAAIAAAPNNSNSRFYIHVKPGIYKEHVEVESQKTFIALIGEDAA 85
           N  V+Q+GTG++ +IG AI+AAPN+S +RF I++K G Y E++E+  +KT I  IG+   
Sbjct: 207 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 266

Query: 86  KTVIVDNRSNGTGFGTIESSTFVVSDNNFMAQSITFENSAGPRNGQAVALFNVANFTVYY 145
           +TVI  NRS   G+    S+T  V  + F+A+ ++F N AGP   QAVAL + ++ + YY
Sbjct: 267 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 326

Query: 146 ECIFLGFQDTLFVNRMKSFFKGCDIYGTIDFIFGDGLAVFQDCKIYARL--PNNLVTITA 205
            C F  +QDT++V+  K F++ CDIYGT+DFIFGD   VFQ+C +YAR   PN  +  TA
Sbjct: 327 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 386

Query: 206 ESKESQNEQSGFVFQNFSVMASPELGSKEVNSSQVYLGRPWREYSTVIFMQSFLDHVVNP 265
           + +E+  E +G    +  ++A+P+L   + N  + YLGRPW+ YS  + M+SF+D +V+P
Sbjct: 387 QGRENSREPTGISIISSRILAAPDLIPVQAN-FKAYLGRPWQLYSRTVIMKSFIDDLVDP 446

Query: 266 AGWLAWST--PLNFLFYAEFNNRGPEADLSRRVKWPGFHVLNNLVEATNFTVRIFIDGNT 325
           AGWL W     L  L+Y E+ N GP ++++ RV+WPGF  +  + EA+ F+V  FIDGN 
Sbjct: 447 AGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNK 506

Query: 326 WLPETGVPYRSSL 335
           WL  T +P+   L
Sbjct: 507 WLNSTRIPFTLDL 518

The following BLAST results are available for this feature:

BLAST of Lcy11g014650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXK7	2.5e-80	46.67	Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3...	[more]
Q43062	3.2e-80	47.25	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]
Q9SMY7	5.1e-78	46.18	Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	4.3e-77	44.76	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
O04887	3.7e-76	44.41	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]

BLAST of Lcy11g014650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAG5	7.4e-112	59.57	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1	[more]
A0A6J1D863	9.0e-110	59.88	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1	[more]
A0A6J1D381	1.7e-108	59.64	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1	[more]
A0A6J1GM54	3.8e-108	60.86	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1	[more]
A0A6J1KPG6	3.5e-106	57.72	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1	[more]

BLAST of Lcy11g014650 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6584282.1	1.6e-116	65.37	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG6584281.1	2.7e-116	65.07	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG7019877.1	1.2e-111	66.12	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
XP_022150332.1	1.5e-111	59.57	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_023511664.1	1.4e-109	61.24	probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy11g014650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G43270.1	1.7e-81	46.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33220.1	3.6e-79	46.18	pectin methylesterase 44	[more]
AT1G02810.1	3.1e-78	44.76	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	1.7e-76	44.84	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02320.1	1.7e-76	43.45	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 26..320 e-value: 9.5E-104 score: 346.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 24..334 e-value: 7.2E-110 score: 369.2
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 24..332
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 24..332
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 51..70
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 170..179
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 26..331

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy11g014650.1	Lcy11g014650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity