Lcy11g012580 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy11g012580
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	dihydrofolate synthetase isoform X1
Location	Chr11: 18197533 .. 18202604 (-)
RNA-Seq Expression	Lcy11g012580
Synteny	Lcy11g012580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATATCTTCAAACATCATCACCACTGTCGCCCACAGATTCTCGGAAGGCTTCTCCTAAATTATTTTGCTGGTGAAAGGCCTTCTATCAGTCGAAGAATAGGGTCCAAACAATGCTTCGGCACTCCCTCAGAAGATCAAGAGATGACGGAGTTCATGGAATACTTAGATTCCCGGAAGAATTATGAGAAATTGGGTGTGCCCAGAGGCGCAGGGACGGAGTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGATGATGGAGCGGCTGGGCAATCCACAATCGAAGTTTAAGGTTGGTTCATTATGAAGATGAAATTTTTAGTTTTCTATTTTTAGTGTTAAGTAAATCTTGCAGTTTTGGACTGACCCATTTCCTCTGCAATTCAACTGAGTTTAATTAGACTTGAAAGCGTATGGTAAATCAGGCTTGGCTACCGTAAGTTAATCTTTTATCCATGTACTTAACATTCTTCCATTGGGTGTAAATTAGCATTTTTTAGTTCAACTACCACCTAGGAGCAGAGATTCAAACCTTTTGGTCAAGGAAAAAAAGGATATAATGTTTTAACTAGTTGTGCTATGATCAGACTAAATCCAAACATATATAATGTGTTAACTTGTTGGTTTATGCTCTGAGCTAAATCCATATGTGTAAATTAGTACGTGACCCTAGTGATTATCCATATACAATATCAATGGGAAAGAACGACATTGAACCTAAAAGTTCGAGTTGGTAGGCATAGTAAACTTAATTGCTTATTCATTCATATTCTTGTATTTGCACCTAGAGACTAATGGTAGAATAATTATAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCCACTGCTGCCTTCCTATCTAACATTTTACGGGCAGAGGGATACTCTGTTGGCTGTTATACTAGGTACACCCTTGTTGCCATGATTATCGAAATTTATGTACACTCTTACTTGGGAGTGATTTGAGAAGGCATAACTTTGTGTCCGTGCATTTCTTTATGTGCAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGAAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAATTTCTTGACCAGTCAGTTGAACTTGAAAATGGACATATTAGTCACTTTGAGGTATTAATTCATGGGGGTCTGTTGTTGTGATTGAAAAATCTCTAAGAACCCAAATGGCATATATGCCGCTTCTGATTCTACCTGATTAATTTAAGCAAAATGATGTTCCAACTAGATTTTCACTTTGTAAATTTGTCCGATGATCTTTTATTTCCCACTGGATATGGACTTCATACATGTTTGGTATATCCTGCATCCTTTTAGGCTTGGTTTCACCTAGGAAGGCATAAATTGTTCCTGTCAACATTTTGGACCCCTTAAAAGTTCATTGGACCTATGCAGTCTTTAATGTTTCCTTTGATCTTTTAAACGAGCTGATGAAATTCTCCCAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCGCAAGAGAAAGTTGATGTTGCAGTCATTGAGGTTCGCTTTCTTTCAGACTAAATTTTTCTTTGCCAAGTATTTTTTATTTCTCTAGCAAAAGCTTTTAACACCATTGACAATATGCTCTTATTTTAACCGAAACAGGCTGGACTTGGTGGTGCACGAGATGCAACGAACATAATTTGCAGCTCCAAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCCGCGCTGGGTGGCTCTTTAGAAAGCATTGCAACAGCAAAGGCTGGAATAATTAAACATGGTTGTCCAGTTAGGTTGTTTTCTTGATGTAATTTTGATGCTGATTGACTTTGTTGATCCTTCGATATCTATTATCCATGCATTAGTTAAGTTTTTCCTTTTCTAATGAGTTGACATGATCTACAAGAAGAGAAGAGGAAACTTGAAGTCAACCTGAACTTAGTGCATGTTTGGGAGTGATTTTAAAATGGTCAAAATCACTTTTATTATATTCAAAATCACTCCGAAACATACCTTTAATAATTCAAAATTAATTTTGATGGTATGAAATCACATTTAAAAGTGTAAAATCAAACATAAAATTGATTTTGAATAATTAAAAGTATGTTTCGGGGTAATTTTGAACATGACAAAAGTGATTTTAGTCATTTTAAAATCACTTCCAAACATGCATTTACATAAGACAACGGAGATCACAAACATGCCCTCCAGTTAGCCTTAATTCTTGTTTCAAGTAATAATAATTGTTGAAAGATTAAATTGCAGTTTTTGTCATAATTGACTTTAGGCATAAGAAAACTGAATTGCGAAGGTATCAAATTGGTTTATTGCTGCAAACGACCTAAAAGCAACCTGCATGAAAAGTTCTTAGAGACTTCCAAAGTCCATTCCCTGGTATTCGGCCTCTAGGGGCATGGAGTTTTCTTTTCTGAAGTTGATGTATATGTATGTGTTTGTGTGTCTGTGTCTATGTGTGAGAGAGAAGCTTATTCTCCAACTTCTATTGTGATAATATTGTCCTTTTCATTTATGTGATTTCTCTTCTGAAATTTATTCCTAAAATGCTCCTTACATCTGATTTCAGACCATTCTGGGTGGTCCTTTCCTTCCAAGCATTGAGTGCATCCTTCACGATAAAGCATTGTCCATGTCTTCGTCTGTATTATATCAGCCTCAGATCCTGGAAATAAAAGTACCATAAAAGGTGTAAACCTGCTCAATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAAGTATGGCTTATGTATTCTATTAAACAAATATAAATTTCACTCCTTCAACTTCTAGCAAGATGAGTTCCTTAATCTCATTGTCAATGCAGTTCATAGAGTTATTGGATGTCCACCTCCGCATGCTTGGAAGTCATCAACTTCAGAATGCAGCAACCGCAACTTGTGTTATTCTTACTCTCCGCAATTTAGGTATAATATCATTTTGCATATTTGATTTGATATCCTACCTTCATTTAGAGAATAAACATGTGATGTGGTGTGATGATTTCCCTGGCACGTTTACTGAATATAATAGCCAGTGTGTGTTGCATCTATACTTTCTTTTAAAAAATTTCGAATGATTGATGCTGTTTGCATGCAAAAGCTCTCTGCTGACGCTTTAATTAGTGTCTGACATCAGTTGTTCTGCTAAAGTATAAGAGAAAGATGTAGTTACTTACTATTGGGTGCTTTAGATGATACATGATGCAAGTTACGTTGTAGTTGATTTCACTGAATACTCTCTCTTTGTATTTTCCTACTCTGTTAAATAATTCAGGTTGGAGAATTTCAGAGGCATCAATTAGGAATGGACTAGAGCAAACATTCTTGGTTGGTAGGAGTCATTTCTTATCAGCTGAGGAAGCTGAGATTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTTCATTCATATTTCTTTTTATTAACGTCAAGTTTGGTCTGTTGGGATCTGGGCATCGTACTCATGCCGCATCTCCTCTACTGTATTTTACTTGAGACTTTTGACAATTTTCTGTTAAGTTCAAACGTGGGTGCTGTTGGTACATGTTTCTAGTTCATGGATTGTTGATTGCTTAAATATTTGTGAATGTTCTAGATCTTCTTTGTGTTAATCTAATGGATTTTAGCTGATAAAATTTCAACTTGTGTCGGCTGATATGTAGTGGAGTTTACACTGTTCTGTTCTGGTCTGGATGTTAGTCCCAAGTCTTACATTCTTTAGATAGCATTTCTGACTGTTGCTTGAATGTTTTCTCAGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGACACCATTCAGATGACTTTTCCAGAGGCACGATTGGCTCTTGTGGTTGCAATGGCTAGTGATAAGGATCACATTGGTTTTGCCAGAGAATTTCTTAAAGGTATAGCCTTCCACTTCAAAGACTAGGCCCCATATTTTCTTGGATGTAAAAGTTTCTTCTTTCTATTCACCGAGTTTGAAACTGAAGATGAAGAATATTGCTTGATTGTAAAATCTTCTATACTCGTGAATTTTTGAATTATATAGGACTTGTTTGATAACCATGTTGCTTTTAGATCATATGAGTGTGTGTGTCTATAGGTTATCTTAGATTGAAATAGATGACCAAAAGAAAGTGTGTAAACAGAAATTGGCAAGAGAAAATACATGTTTAAATAGAGTAAATAAATTAGAACGGTTAGCTTTAGTTTTATGTAATTTGGGTTTTAGATTTTTTTCTTTTTTTTTTTTTCTTAAATATTAGTTAGTGTTTCAAAATTTTTATTAAACAAAGCTTAAAAAACAGAAACGGAAACAAAATTATCTTCTTTATATCTTGGGCAGGAGGCCTAAGAGAGATCTCAAGTCTTTTATCAAATTACAGGCTTCCTATTCTATCAATTCTGCTTTATTTTTTTTAACAAGACTCGAAAACTTTCATTGAAGTAGTGAAAAGAAACAGTTGTTTAGAGAAAAAGAAATAAACTAAATGAGGTCTATGAAAGCACCCCAATTAATACAAATATCCCAAGAAGCGTAATAAAAAACACTTTAGAAAGAGAACACCGTCGAGAAGCTTTTAATATAGAAATATCTTGTTTTTCTATATCTTCTCTAATGTTGTAAGATGTAGCAATGAAATGTTGACAGTCAGCAATTAACTGAATTTCAGGTGGAAAATTGGAGTCCATCGTCTTAACTGAAGCCAATATTGGTGGAGCCAAATCAAGGACAACTTCAGCTGCTCTTCTAAGGGACTGCTGGATTCAAGCATCCAATGAACTGGGGATCCCTGTTTCTCTAGAAACTAAAGATGCATCGGTATCCTCCATGAGCAAACTCGAAAACAGGCCGATATTGACCACCGAGACCTCGTTATTACATGCCATAAAAATCACTGTTGAGAATCTCAAGCAAAGAACGGAAGGGCAGCAACGAGGTCTAATCGTAGTAACTGGATCGCTGCACGCGGTTTCAATGGTGTTATCTTCTATTCATTCTTGA

mRNA sequence

ATGAATATCTTCAAACATCATCACCACTGTCGCCCACAGATTCTCGGAAGGCTTCTCCTAAATTATTTTGCTGGTGAAAGGCCTTCTATCAGTCGAAGAATAGGGTCCAAACAATGCTTCGGCACTCCCTCAGAAGATCAAGAGATGACGGAGTTCATGGAATACTTAGATTCCCGGAAGAATTATGAGAAATTGGGTGTGCCCAGAGGCGCAGGGACGGAGTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGATGATGGAGCGGCTGGGCAATCCACAATCGAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCCACTGCTGCCTTCCTATCTAACATTTTACGGGCAGAGGGATACTCTGTTGGCTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGAAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAATTTCTTGACCAGTCAGTTGAACTTGAAAATGGACATATTAGTCACTTTGAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCGCAAGAGAAAGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACGAACATAATTTGCAGCTCCAAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCCGCGCTGGGTGGCTCTTTAGAAAGCATTGCAACAGCAAAGGCTGGAATAATTAAACATGGTTGTCCATTCATAGAGTTATTGGATGTCCACCTCCGCATGCTTGGAAGTCATCAACTTCAGAATGCAGCAACCGCAACTTGTGTTATTCTTACTCTCCGCAATTTAGGTTGGAGAATTTCAGAGGCATCAATTAGGAATGGACTAGAGCAAACATTCTTGGTTGGTAGGAGTCATTTCTTATCAGCTGAGGAAGCTGAGATTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGACACCATTCAGATGACTTTTCCAGAGGCACGATTGGCTCTTGTGGTTGCAATGGCTAGTGATAAGGATCACATTGGTTTTGCCAGAGAATTTCTTAAAGGTGGAAAATTGGAGTCCATCGTCTTAACTGAAGCCAATATTGGTGGAGCCAAATCAAGGACAACTTCAGCTGCTCTTCTAAGGGACTGCTGGATTCAAGCATCCAATGAACTGGGGATCCCTGTTTCTCTAGAAACTAAAGATGCATCGGTATCCTCCATGAGCAAACTCGAAAACAGGCCGATATTGACCACCGAGACCTCGTTATTACATGCCATAAAAATCACTGTTGAGAATCTCAAGCAAAGAACGGAAGGGCAGCAACGAGGTCTAATCGTAGTAACTGGATCGCTGCACGCGGTTTCAATGGTGTTATCTTCTATTCATTCTTGA

Coding sequence (CDS)

ATGAATATCTTCAAACATCATCACCACTGTCGCCCACAGATTCTCGGAAGGCTTCTCCTAAATTATTTTGCTGGTGAAAGGCCTTCTATCAGTCGAAGAATAGGGTCCAAACAATGCTTCGGCACTCCCTCAGAAGATCAAGAGATGACGGAGTTCATGGAATACTTAGATTCCCGGAAGAATTATGAGAAATTGGGTGTGCCCAGAGGCGCAGGGACGGAGTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGATGATGGAGCGGCTGGGCAATCCACAATCGAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCCACTGCTGCCTTCCTATCTAACATTTTACGGGCAGAGGGATACTCTGTTGGCTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGAAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAATTTCTTGACCAGTCAGTTGAACTTGAAAATGGACATATTAGTCACTTTGAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCGCAAGAGAAAGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACGAACATAATTTGCAGCTCCAAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCCGCGCTGGGTGGCTCTTTAGAAAGCATTGCAACAGCAAAGGCTGGAATAATTAAACATGGTTGTCCATTCATAGAGTTATTGGATGTCCACCTCCGCATGCTTGGAAGTCATCAACTTCAGAATGCAGCAACCGCAACTTGTGTTATTCTTACTCTCCGCAATTTAGGTTGGAGAATTTCAGAGGCATCAATTAGGAATGGACTAGAGCAAACATTCTTGGTTGGTAGGAGTCATTTCTTATCAGCTGAGGAAGCTGAGATTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGACACCATTCAGATGACTTTTCCAGAGGCACGATTGGCTCTTGTGGTTGCAATGGCTAGTGATAAGGATCACATTGGTTTTGCCAGAGAATTTCTTAAAGGTGGAAAATTGGAGTCCATCGTCTTAACTGAAGCCAATATTGGTGGAGCCAAATCAAGGACAACTTCAGCTGCTCTTCTAAGGGACTGCTGGATTCAAGCATCCAATGAACTGGGGATCCCTGTTTCTCTAGAAACTAAAGATGCATCGGTATCCTCCATGAGCAAACTCGAAAACAGGCCGATATTGACCACCGAGACCTCGTTATTACATGCCATAAAAATCACTGTTGAGAATCTCAAGCAAAGAACGGAAGGGCAGCAACGAGGTCTAATCGTAGTAACTGGATCGCTGCACGCGGTTTCAATGGTGTTATCTTCTATTCATTCTTGA

Protein sequence

MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRKNYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPFIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASIRNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIPVSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAVSMVLSSIHS

Homology

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BLAST of Lcy11g012580 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 8.2e-133
Identity = 269/509 (52.85%), Postives = 341/509 (66.99%), Query Frame = 0


Query: 44  SEDQEMTEFMEYLDSRKNYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHI 103
           +ED E+ +F+ +L+S KNYEK GVP+GAGT+SDDGFDLGRM+R+M RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +E VD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIATAKAGIIKHG---------------------------- 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG                            
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 --------------------------C-----------PFIELLDVHLRMLGSHQLQNAA 343
                                     C           P +EL DV+LRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCVILTLRNLG-WRISEASIRNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHT 403
           TATCV L LR+ G  R+++ +IR GLE T L+GRS FL+ +EAE L LPGAT+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399

Query: 404 KDSAKALVDTIQMTFPEARLALVVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKS 463
           K+SA+AL + I+  FPE RL  VVAMASDKDH+ FA+E L G K E+++LTEA+IGG K 
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459

Query: 464 RTTSAALLRDCWIQASNELGIPVSLETKDASVSSMSKLENRPILTTETSLLHAIKITVEN 487
           R+T +++L++ WI+A++ELG             SM   EN+       ++L ++K+  + 
Sbjct: 460 RSTESSVLKESWIKAADELG-----------SRSMEASENK-------TVLGSLKLAYKI 519

[Close]

BLAST of Lcy11g012580 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 1.9e-41
Identity = 111/343 (32.36%), Postives = 173/343 (50.44%), Query Frame = 0


Query: 81  LGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRM+++M RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQ-EKVDV 200
           RIS+  +G  +S +   +L  + K  ++   + E G  + FE++TA AF  FA+  KVD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 201 AVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPFI 260
            + E GLGG  D+TN++   +    VITSIG +HM  LG ++E IA  KAGIIK G P +
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIV 203

Query: 261 ELL---------------------------------------------------DVHLRM 320
             +                                                   D+   +
Sbjct: 204 TAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGEQFSFKTEEKCYEDIRTSL 263

Query: 321 LGSHQLQNAATATCVI--LTLRNLGWRISEASIRNGLEQTFLVGRSHFLSAEEAEILGLP 370
           +G+HQ QNAA +      L   N+   IS+ ++R+GL +    GR   +           
Sbjct: 264 IGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGRLELVQEH-------- 323

[Close]

BLAST of Lcy11g012580 vs. ExPASy Swiss-Prot
Match: D4GW05 (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=folCP PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.2e-28
Identity = 102/351 (29.06%), Postives = 151/351 (43.02%), Query Frame = 0


Query: 72  GTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYT 131
           GTES        +RR++  LG+P      + +AG+ GKGSTA  +  +LR  G  VG YT
Sbjct: 21  GTES--------IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYT 80

Query: 132 SPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSL 191
           SPH + +RER+ ++  G  +   AL++     K +L +    +   ++ FE +TA+A   
Sbjct: 81  SPHFDDVRERVRVD--GRKIPKSALSAFVAEAKPYLVERA-ADGEPLTFFETVTALALWY 140

Query: 192 FAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGI 251
           F +  VDVAV+E G+GG  DAT+ +      A+ +T++  EH A LG ++  IA  KA +
Sbjct: 141 FDRAGVDVAVLEVGMGGELDATSAV---DPVASAVTNVSLEHTAVLGDTVAEIAKTKAAV 200

Query: 252 IKHGCPFI---------------------------------------------------- 311
                P +                                                    
Sbjct: 201 APADAPLVTGTTGEALSVIREEAGDVLTVGDADADSDADVRVSYGGRVNHQEAAVTVETD 260

Query: 312 -ELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASIRNGLEQTFLVGRSHFLSAE 370
            E LDV + +LG++Q +NA  A  +   +R     I   +I  GL      GR   +  E
Sbjct: 261 AETLDVRIPLLGAYQARNAGIAVSLARQVRP---DIDAEAIHRGLRNAHWPGRFEVMGTE 320

[Close]

BLAST of Lcy11g012580 vs. ExPASy Swiss-Prot
Match: P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)

HSP 1 Score: 127.1 bits (318), Expect = 5.4e-28
Identity = 110/338 (32.54%), Postives = 155/338 (45.86%), Query Frame = 0


Query: 75  SDDGFDLGRMRRMMERLG---NPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYT 134
           SD    L  M   + R G      ++   IH+ GTKGKGST AF   ILR  G   G ++
Sbjct: 70  SDPQAQLEAMEMYLARSGLQVEDLNRLNIIHVTGTKGKGSTCAFTERILRNYGLKTGFFS 129

Query: 135 SPHIETIRERISLERSGEMVSGKALNS----LFKRNKEFLDQSVELENGHI---SHFEVL 194
           SPH+  +RERI +  +G+ +S +        L+ + +EF D S      H+   S+F  L
Sbjct: 130 SPHMVQVRERIRI--NGKPISPELFTKHFWCLYNQLEEFKDDS------HVSMPSYFRFL 189

Query: 195 TAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESI 254
           T MAF +F QEKVD+AV+E G+GGA D TNII   K     ++S+G +H + LG ++E I
Sbjct: 190 TLMAFHVFLQEKVDLAVVEVGIGGAFDCTNII--RKPVVCGVSSLGIDHTSLLGDTVEKI 249

Query: 255 ATAKAGIIKHG-----------------------------CPFIELLD-----VHLRMLG 314
           A  K GI K G                             CP +E L+     + L + G
Sbjct: 250 AWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCPPLEALEEVGLPLSLGLEG 309

Query: 315 SHQLQNAATAT----CVI------------LTLRNLGWRISEASI-------RNGLEQTF 346
           +HQ  NAA A     C +            ++  ++ W++  A +       R GL  T 
Sbjct: 310 AHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAPVFRPTPHMRRGLRDTV 369

[Close]

BLAST of Lcy11g012580 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.4e-28
Identity = 96/347 (27.67%), Postives = 161/347 (46.40%), Query Frame = 0


Query: 80  DLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIR 139
           D  R+  ++  LGNPQ + + IH+ GT GKGS A  ++++L A G +VG YTSP I    
Sbjct: 21  DHRRILTLLHALGNPQQQGRYIHVTGTNGKGSAANAIAHVLEASGLTVGLYTSPFIMRFN 80

Query: 140 ERISLERSGEMVSGKALNSLFKRNKEFLDQSVELE-NGHISHFEVLTAMAFSLFAQEKVD 199
           ERI ++   E +   AL +     +  L++  + + + +++ FE +TA+ +  F Q +VD
Sbjct: 81  ERIMIDH--EPIPDAALVNAVAFVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVD 140

Query: 200 VAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPF 259
           VAVIE G+GG  D+TN+I       +V+T +  +H   LG ++ +IA  KAGIIK G P 
Sbjct: 141 VAVIEVGIGGDTDSTNVITP---VVSVLTEVALDHQKLLGHTITAIAKHKAGIIKRGIPV 200

Query: 260 I---------------------------------------------------ELLDVHLR 319
           +                                                    + D+ + 
Sbjct: 201 VTGNLVPDAAAVVAAKVATTGSQWLRFDRDFSVPKAKLHGWGQRFTYEDQDGRISDLEVP 260

Query: 320 MLGSHQLQNAATA-TCVILTLRNLGWRISEASIRNGLEQTFLVGRSHFLSAEEAEILGLP 374
           ++G +Q +N A A     +  +   W ++  +IR GL        SH+  A   +I   P
Sbjct: 261 LVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGL------AASHW-PARLEKISDTP 320

[Close]

BLAST of Lcy11g012580 vs. ExPASy TrEMBL
Match: A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 1.0e-231
Identity = 435/549 (79.23%), Postives = 458/549 (83.42%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+H   RPQILG LLLN+  GE PSIS RIGSKQCF T SED E+TEFMEYLDS K
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGT+SDDGFDLGRMRR+MERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISL RSGEMVSGKALNSLFKRNKEFLDQSVE ENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIA AKAGIIKHGCP                                           
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIELLDV+LRMLG HQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           + GLEQTFLVGRSHFLSA+EA +LGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH+GFAREFL+GGKLESI+LTEANIGG KSRTTSAA+LRDCWIQA+NELGIP
Sbjct: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA VSS SKLE+RPILTTETSLLHA+KI  E LKQRTEG + GL VVTGSLHAV
Sbjct: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEG-RAGLTVVTGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. ExPASy TrEMBL
Match: A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.4e-230
Identity = 426/549 (77.60%), Postives = 458/549 (83.42%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+HHHCRPQILG LLLN+F GE PSIS R GSKQCF T SEDQ MT+FMEYLDS K
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GT+SDDGFDLGRMRR+MERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           R EGYSVGCYTSPHIETIRERISL RSG+MVSGKALNSLFKRNKE  DQSVELENGH+SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIATAKAGIIK GCP                                           
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIELLDV+LRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLEQTFL+GRSHFL+A EAE+LGLPGATILLDGAHTKDSAKAL+DTIQM FPEA+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDK+H+GFAREFL+GGKLES+VLTEA IGG KSRTTSAA LRDCWIQASNELGIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA V   SKL NRP+LTTETSLLHAIKI  E LKQRT+G+Q GL+VV+GSLHAV
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQ-GLVVVSGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. ExPASy TrEMBL
Match: A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.7e-229
Identity = 431/549 (78.51%), Postives = 457/549 (83.24%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+HHH RPQI GRLLLNYF GE PSIS RIGSKQCF   SEDQ+MTEFMEYLDS K
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+R+MERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISL RSGEMVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIA AKAGIIKHGCP                                           
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIEL DV+LRMLG HQLQNAATATCVILTLRNLGWRISEASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLE+TFLVGRSHFLSA+EA ILGLPG  ILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+GGKLESIVL+EANIGG+KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA VSS SKLENRP+LTTETSLL AIKI  E LKQR +G +RGL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDG-RRGLVVVTGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. ExPASy TrEMBL
Match: A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.8e-229
Identity = 428/549 (77.96%), Postives = 455/549 (82.88%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+HHHCRPQILG LLLN+F GE PSIS R GSKQCF T SEDQ MTEFMEYLDS K
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGT+SDDGFDLGRMRR+MERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIET+RERISL RSGEMVSGKALNSLFKRNKE  DQSVELE+G ISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIA AKAGIIK GCP                                           
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             F+ELLDV+LRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLEQTFL+GRSHFL+A+EAE+LGLPGATILLDGAHTKDSAKALVDTIQM FPEARLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           V+AMASDK+H+ FAREFL+GGKLE +VLTEA+IGG KSRTTSAA LRDCWIQAS ELGIP
Sbjct: 421 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA VS  SKLENRP+LTTETSLLHAIKI  E LKQRT+GQQ  L+VV+GSLHAV
Sbjct: 481 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQ-SLVVVSGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. ExPASy TrEMBL
Match: A0A6J1J6Z7 (dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.6e-216
Identity = 415/549 (75.59%), Postives = 439/549 (79.96%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+HHH R QILGRLLLNYF GE PSIS RIGSKQCFG  SEDQ+MTEFMEYLDS K
Sbjct: 1   MNVFKYHHHFRLQILGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+R+MERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISL RSGEMVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSILAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIA AKAGIIKHGCP                                           
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIEL DV+LRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGRLCQCCDLIIQTDDEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLE+TFLVGRSHFLSA+EA +LGLPG TILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+ GKLESIVL+EANIGG KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHNGFATEFLQCGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
                               +LTTETSLL AIKI  E LKQRTEG +RGL+VVTGSLHAV
Sbjct: 481 TQTS----------------LLTTETSLLRAIKIAAEILKQRTEG-RRGLVVVTGSLHAV 532

[Close]

BLAST of Lcy11g012580 vs. NCBI nr
Match: XP_022141068.1 (dihydrofolate synthetase isoform X1 [Momordica charantia])

HSP 1 Score: 812.4 bits (2097), Expect = 2.1e-231
Identity = 435/549 (79.23%), Postives = 458/549 (83.42%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+H   RPQILG LLLN+  GE PSIS RIGSKQCF T SED E+TEFMEYLDS K
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGT+SDDGFDLGRMRR+MERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISL RSGEMVSGKALNSLFKRNKEFLDQSVE ENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIA AKAGIIKHGCP                                           
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIELLDV+LRMLG HQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           + GLEQTFLVGRSHFLSA+EA +LGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH+GFAREFL+GGKLESI+LTEANIGG KSRTTSAA+LRDCWIQA+NELGIP
Sbjct: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA VSS SKLE+RPILTTETSLLHA+KI  E LKQRTEG + GL VVTGSLHAV
Sbjct: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEG-RAGLTVVTGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. NCBI nr
Match: XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])

HSP 1 Score: 811.2 bits (2094), Expect = 4.8e-231
Identity = 434/549 (79.05%), Postives = 454/549 (82.70%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           M IFK+HHHCRPQILG LLLN+F GE PSIS RIGSKQCF T  EDQ MTEFMEYLDS K
Sbjct: 1   MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP+GAGT+SDDGFDLGRMRR+MERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYS GCYTSPHIETIRERISL RSGEMVSGKALNSLFKRNKEF DQSVELENG ISH
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAF LFAQE VD+AVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIATAKAGIIK GCP                                           
Sbjct: 241 LESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVISASGPGNRSTIKGVSL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIELLDV+LRMLG HQLQNAATATCVIL LRNLGWRIS+AS+
Sbjct: 301 LNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCVILALRNLGWRISDASV 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLEQTFLVGRSHFL A+EA +LGLPGATILLDGAHTKDSAKALVDTIQM FPEARLAL
Sbjct: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDHIGFAREFL+GGKLESIVLTEA+IGG KSRTTSA  LRDCWIQASNELGIP
Sbjct: 421 VVAMASDKDHIGFAREFLQGGKLESIVLTEAHIGGGKSRTTSAVFLRDCWIQASNELGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA VS  SKLENRP+LTTETSLLHAIK+  E LKQRTEG+Q GL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSFTSKLENRPVLTTETSLLHAIKVAAEILKQRTEGRQ-GLVVVTGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. NCBI nr
Match: KAG6576673.1 (Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808.9 bits (2088), Expect = 2.4e-230
Identity = 433/549 (78.87%), Postives = 457/549 (83.24%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+HHH RPQI GRLLLNYF GE PSIS RIGSKQCFG  SEDQ+MTEFMEYLDS K
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTES+DGFDLGRM+R+MERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISL RSGEMVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIA AKAGIIKHGCP                                           
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIEL DV+LRMLG HQLQNAATATCVILTLRNLGWRISEASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLE+TFLVGRSHFLSA+EA ILGLPG  ILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+GGKLESIVL+EANIGG KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA VSS SKLENRP+LTTETSLL AIKI  E LKQR +G +RGL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDG-RRGLVVVTGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. NCBI nr
Match: XP_004149457.1 (dihydrofolate synthetase isoform X2 [Cucumis sativus])

HSP 1 Score: 807.0 bits (2083), Expect = 9.0e-230
Identity = 426/549 (77.60%), Postives = 458/549 (83.42%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+HHHCRPQILG LLLN+F GE PSIS R GSKQCF T SEDQ MT+FMEYLDS K
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GT+SDDGFDLGRMRR+MERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           R EGYSVGCYTSPHIETIRERISL RSG+MVSGKALNSLFKRNKE  DQSVELENGH+SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIATAKAGIIK GCP                                           
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIELLDV+LRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLEQTFL+GRSHFL+A EAE+LGLPGATILLDGAHTKDSAKAL+DTIQM FPEA+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDK+H+GFAREFL+GGKLES+VLTEA IGG KSRTTSAA LRDCWIQASNELGIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA V   SKL NRP+LTTETSLLHAIKI  E LKQRT+G+Q GL+VV+GSLHAV
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQ-GLVVVSGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.8 bits (2080), Expect = 2.0e-229
Identity = 431/549 (78.51%), Postives = 458/549 (83.42%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSISRRIGSKQCFGTPSEDQEMTEFMEYLDSRK 60
           MN+FK+HHH RPQI GRLLLNYF GE PSIS RIGSKQCFG  SEDQ+MTEFMEYLDS K
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+R+MERLGNPQSKFKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISL RSGEMVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCP------------------------------------------- 300
           LESIA AKAGIIKHGCP                                           
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 ------------------FIELLDVHLRMLGSHQLQNAATATCVILTLRNLGWRISEASI 360
                             FIEL DV+LRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           R+GLE+TFLVGRSHFLSA+EA +LGLPG TILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+GGKLESIVL+EANIGG KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 VSLETKDASVSSMSKLENRPILTTETSLLHAIKITVENLKQRTEGQQRGLIVVTGSLHAV 489
           +SLETKDA VSS SKLENRP+LTTETSLL AIKI  E LKQR EG +RGL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIEG-RRGLVVVTGSLHAV 540

[Close]

BLAST of Lcy11g012580 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 475.3 bits (1222), Expect = 5.8e-134
Identity = 269/509 (52.85%), Postives = 341/509 (66.99%), Query Frame = 0


Query: 44  SEDQEMTEFMEYLDSRKNYEKLGVPRGAGTESDDGFDLGRMRRMMERLGNPQSKFKAIHI 103
           +ED E+ +F+ +L+S KNYEK GVP+GAGT+SDDGFDLGRM+R+M RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLERSGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSKLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +E VD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIATAKAGIIKHG---------------------------- 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG                            
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 --------------------------C-----------PFIELLDVHLRMLGSHQLQNAA 343
                                     C           P +EL DV+LRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCVILTLRNLG-WRISEASIRNGLEQTFLVGRSHFLSAEEAEILGLPGATILLDGAHT 403
           TATCV L LR+ G  R+++ +IR GLE T L+GRS FL+ +EAE L LPGAT+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399

Query: 404 KDSAKALVDTIQMTFPEARLALVVAMASDKDHIGFAREFLKGGKLESIVLTEANIGGAKS 463
           K+SA+AL + I+  FPE RL  VVAMASDKDH+ FA+E L G K E+++LTEA+IGG K 
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459

Query: 464 RTTSAALLRDCWIQASNELGIPVSLETKDASVSSMSKLENRPILTTETSLLHAIKITVEN 487
           R+T +++L++ WI+A++ELG             SM   EN+       ++L ++K+  + 
Sbjct: 460 RSTESSVLKESWIKAADELG-----------SRSMEASENK-------TVLGSLKLAYKI 519

[Close]

BLAST of Lcy11g012580 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 125.9 bits (315), Expect = 8.6e-29
Identity = 98/282 (34.75%), Postives = 138/282 (48.94%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLERSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E+VDV ++E GLGG  DATN+
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNV 175

Query: 216 ICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPFIEL-------------- 275
           I   K     I+S+G +HM  LG +L  IA  KAGI K G P   +              
Sbjct: 176 I--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKA 235

Query: 276 --LDVHLRML------------GSHQLQNAATATCVILT-LRNLGWRISEA-SIRNGLEQ 335
             L+V+L+++            G HQ  NA  A  +  T L+ +G          NGL +
Sbjct: 236 SKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPE 295

Query: 336 TFLVGRSHFLSAEEAEIL---GLPGATI-LLDGAHTKDSAKA 344
            F+ G S+      A I+    LP   +  LDGAH+ +S +A
Sbjct: 296 KFISGLSNAYLMGRAMIVPDSELPEEIVYYLDGAHSPESMEA 333

[Close]

BLAST of Lcy11g012580 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 124.8 bits (312), Expect = 1.9e-28
Identity = 98/282 (34.75%), Postives = 137/282 (48.58%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLERSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E+VDV ++E GLGG  DATNI
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNI 175

Query: 216 ICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPFIEL-------------- 275
               K     I+S+G +HM  LG +L  IA  KAGI K G P   +              
Sbjct: 176 ---QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKA 235

Query: 276 --LDVHLRML------------GSHQLQNAATATCVILT-LRNLGWRISEA-SIRNGLEQ 335
             L+V+L+++            G HQ  NA  A  +  T L+ +G          NGL +
Sbjct: 236 SKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPE 295

Query: 336 TFLVGRSHFLSAEEAEIL---GLPGATI-LLDGAHTKDSAKA 344
            F+ G S+      A I+    LP   +  LDGAH+ +S +A
Sbjct: 296 KFISGLSNAYLMGRAMIVPDSELPEEIVYYLDGAHSPESMEA 332

[Close]

BLAST of Lcy11g012580 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 113.2 bits (282), Expect = 5.8e-25
Identity = 68/165 (41.21%), Postives = 94/165 (56.97%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLERSGEMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L+  G  +S +  
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLD--GVDISEEKF 170

Query: 157 NSLF----KRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDA 216
              F     R KE  ++ + +     ++F  L  +AF +FA E+VD A++E GLGG  DA
Sbjct: 171 LGYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDA 230

Query: 217 TNIICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCP 258
           TN +   K     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 231 TNAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 267

[Close]

BLAST of Lcy11g012580 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 113.2 bits (282), Expect = 5.8e-25
Identity = 68/165 (41.21%), Postives = 94/165 (56.97%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLERSGEMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L+  G  +S +  
Sbjct: 53  KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLD--GVDISEEKF 112

Query: 157 NSLF----KRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDA 216
              F     R KE  ++ + +     ++F  L  +AF +FA E+VD A++E GLGG  DA
Sbjct: 113 LGYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDA 172

Query: 217 TNIICSSKLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCP 258
           TN +   K     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 173 TNAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 209

The following BLAST results are available for this feature:

BLAST of Lcy11g012580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	8.2e-133	52.85	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	1.9e-41	32.36	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
D4GW05	3.2e-28	29.06	Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha...	[more]
P48760	5.4e-28	32.54	Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1...	[more]
P15925	5.4e-28	27.67	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]

BLAST of Lcy11g012580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJG1	1.0e-231	79.23	dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115...	[more]
A0A0A0L7A4	4.4e-230	77.60	Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454...	[more]
A0A6J1E819	1.7e-229	78.51	dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A1S3CNR7	2.8e-229	77.96	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3...	[more]
A0A6J1J6Z7	1.6e-216	75.59	dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 ...	[more]

BLAST of Lcy11g012580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141068.1	2.1e-231	79.23	dihydrofolate synthetase isoform X1 [Momordica charantia]	[more]
XP_038905573.1	4.8e-231	79.05	dihydrofolate synthetase [Benincasa hispida]	[more]
KAG6576673.1	2.4e-230	78.87	Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_004149457.1	9.0e-230	77.60	dihydrofolate synthetase isoform X2 [Cucumis sativus]	[more]
XP_023553540.1	2.0e-229	78.51	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy11g012580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	5.8e-134	52.85	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	8.6e-29	34.75	DHFS-FPGS homolog D	[more]
AT3G55630.2	1.9e-28	34.75	DHFS-FPGS homolog D	[more]
AT5G05980.1	5.8e-25	41.21	DHFS-FPGS homolog B	[more]
AT5G05980.2	5.8e-25	41.21	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 103..254 e-value: 7.8E-9 score: 35.7
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 81..259 e-value: 1.2E-65 score: 220.4
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 258..484
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..259
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 320..488 e-value: 6.7E-18 score: 67.3
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 321..486
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 41..258 e-value: 1.6E-72 score: 246.1
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 259..308 e-value: 5.1E-7 score: 30.9
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 78..307
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 258..484
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..259
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 201..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy11g012580.1	Lcy11g012580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006761	dihydrofolate biosynthetic process
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008841	dihydrofolate synthase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity
molecular_function	GO:0016874	ligase activity