Lcy11g009010 (gene) Sponge gourd (P93075) v1

Overview
NameLcy11g009010
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectinesterase
LocationChr11: 9617436 .. 9623633 (+)
RNA-Seq ExpressionLcy11g009010
SyntenyLcy11g009010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAATACCATTTAAACTGAGAAGTCACAGATGGGTCTTAAATTTCCATGGCCGTTAATGGCGAAACAGACAATTTGAATACAGACACACAGCTGCAAGATTCCTCCAATGTTGGTCTCTCTCTTCCCCATTTCTGCATTTTTTTCAACTCTTTTAAGCCTCTCATTCTCTCAAACGCTTATCTACACTGCAATCGCAGATATCACGACGCACTTGCTGGTTTAACCATGGCTGCCCAGAGCTGCTTCGAGAAGATGAGGGTGGTGAAGCTTTTGGTTCTGGTGGTGATGATACTGCCGGGCGCCGCCGTTTCTTACGGCCGAAACTGGACGGTCGGCGGCGGCGGGGGAATGAGAAAGGTTGAAGATGAGTACTTGAAGTGGATAAGGCATATGGGTTCTTTCAAGCACTCGCTTTTTCAGAAGACGAAGAACAAGTTCAAGCCTTGTTTGACATTGAAGGTTTCGAAGAAACCCAAGGCGGGAGGTTTCCGGTCGGTGCAGAAGGCTATAAACTCTCTGCCGGTGGTCAACCGGTGCCGGGTCGTCATCCATGTCGCCGCCGGAATCTACAGGTGAGCTTCCTTTGATTCATTTTAAAAGCTTTTGTTGGAATGGCTTTTAAAAAGTATATACATATTTTTTAGGGATGGTATTAAAAAGAAAAAAAGATTGATAAAATATTTTGGTTAAATTATAATTTTATTTTCTTTCCTTTTGAAATTTTAATTTTGTATATATTAGGTTTTTAACTTTCTATAAACTAATAAGTCAATGATATATTTAATATATTTTTTAATATTTTCGAACTTATTAAATACAAAATTGAAAATTTATGAATATTAATGGATAGTATCTCAATGATCACATTTTTCAAAGTTTGTAAAATAAGAAAAAAAGACATGCTAGATAATTCAAGAATTTAACTTGTAAAAATATTTATTCTTTTTCCTAGTATTTTTGGAGTTTTCAAAATTATTATCTAACCAAAAATCTTGAATTTGTTTTTAATGGACTAAAAAAAACCACTTTAATTTTTACCTTTTGTTCCAAACTCGAGTCTTTTGATACTAAATTTGGATTGAGAAGAGGAAAAATTGAACTTTTATATCATAATGGAAAATGGATCGAAGAAGTTAATTAAGACATATACTTTTGGACAAGATATCAAATCAAATGTACAAATCTCTCACCTGTACATGTTGTTCAGAGAGAAGGTTGAGATTCCGGCAACGATGAGTTACATATGGGTGGAGGGGGAAGGAGCGGAGAAGACAATCATTGAATGGGGCGACACGGCTGATCATATGGGCGACAATGGCCGTCCCATGGGCACTTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCTTTCTTCATTGCCAGGAACATCACCTTCAAGGTAAACCTTTTCAATCGATTTGCAAGCAAACAAAAAGTTAAAATCCGTTCTACCGGACTATTTCTTTTTGAAAATTATGCTTTTTTAAAAAAAAAAATTAGTTTTTATTTTTATGTTAAGGACACATTTTAATTATTTTAACAAAATTCCAGAAGATAAACGAGTTTATCTATCATTTTTAGATTTAAATTCTATTCATCTCTTTAGAATTTTAAATATTTTTGTTGGAATTTTATTACTTTAATATATATATATATATATATATATATATATATATTAAATAACACAAAAGAGAATATCTTTCATGGACTGATTTTTTTTTTTTTTTTTTTTTGATAAAGTTACATGTTTTGTTCAGGACAAATATTTGCAGCCACATCTCATTAGATACTGAATTTTTAAATGATTAATGAATCTTTTTTTTTTTTAAAGCGATCAATAAAGAATTAACGATAAAGACTCAACCATTTTTAATGCAAAGTTTGAAGGACCAAAATAAAATTTTGAAAATTTAGAATCCAAAATAAAATAATTAAGCATGAAAGTGTAAAGTTCAAAGTGACTTAATTAAATGCTCATTTTCATTTTTTCTATCACTAGTCACAAATACTTACGCCATGATTTTTATACATTTTAAAAATATAATAAATCTAAATTAAAATGGTATATCAATATAATTGGTGAAGTTGAAGACAACGTATTGTTTTTACTATTTTTTTTAATGTATAAAACACTTTATCCATTTTTTATTGACGAAAAGAATTGAAAAAACTTTTGTTGCATATCGATGTGTAGAGGTCTCCTTATATATTACCATTAAAAAAATTTAAATGTTGACAACTTGGAGAGATAATTTTTTTTTTCTCATTTTCTACCTTTATCTTTATTAGTTGTCAAGATTTTAATGGGATATGTAAGGAGATTCCTACATTACAGCCAAGTATTGTTCTAAAATGCATGAATATAATTAGAGATTTCCTGATTTTTTTTTTCTCTTTCAAGTTACTCATATCTCTTTCAACTAAAAATGAAAAAGTTAAAAAAAAAAAAAAAAGAAAAAAAGAAAAAAAAAATGGAGTTAGGACAGTATATTTTATTTGACCTAAATATGCGAAGGAATAAAGTTTGAGTGTCGTATGTAAATGGTAATTTGCTTGTGACTTCCGATGACCAAACTGACATGCTTGTATATTTTTTTGTAATACAATGACCAAACTGACATATAGTTCTTTTTTTTTTTTTTTTTTTTTTGAGACGACTGACATATAGTTCTTGGCAGCACTTGACCATTAAAAATATCATAATCATCGAGGTGAAATTGAAAATCTATTCTTTTCACCCAAAAAAAAAGATTATACAAAAGTTTTAATTTCATTATAATATATTTAAGTCCAGTTTTGTACTTTTGTTTTCTTTGTTTATTTGAGAGAGCATAAATTAATATTTATTTAAATGTAGTTTAAAATCTTTATCTTTTTATTTATTTTAGGAAAAAATACATTTTTAGTCCCTGAAGTTTGGATTAGGTTTCATTTTGGTTCCCGAGTTTTCAAACTCGACAATTTTGGATTTCGTTTCATTTTAGTCCATCCATCCATTTGTTCGTTAAATGTTTTGTTGACGTGGCACCTATTATTATTTTTTAAAAAAAATTGTTTTTTTTTAATTTTTAAAATAAAATATTATTAAATTAATTTTTTAAAAAATCTTATTTCTTCCTTCTTTATCTCTCTCTCTTCTTCTTATTCTTCTTCTTCATCATCAACCACCAATGACCGTCACCTGTCGGCTGCCGCCGTCACCGTGGTCGGAGAATTTTTTTTTTTTTTTTCCTCTTCCTTTCTTTTTCTTTTCTTTCTTCTCATTTCCCTTCTTTCTTTCACGTTAAAAAAAAAAAACGAAGGGGGTTAACCCCCTTCGAATCGGGACTGAAACGATCCCCCTTCATTCCAGATCAGTCCCAAGCCTTTTTTTTTAAAACGTGAAAGAAACTCACAAAGGGAAGAGATCCTTGCCGACGAGAAGGTTTCTTTCCCTTCATGAGTTTTTTTTTAAAAAAAAACAATTCATTCCATATCTGGTAGAATCGTTCCAGTCCCGATTCGAAGGGGGTTCAACCCCCTTCTTTTTTTTTTTTTTAAATAAACGTGAAAGAAAAAAATGGAAAGGAGAAGAAAGAAAAGAAGAAGAAAGGAGAGGGAAAAAAAAAAAAAAAAGAAAAAGAAAAAAGAATCTTCGGCAACGGCAGCGACGATGGCCGACGGGTGACGGTCATCGGCGGGTGGTGGCTGATGATGATGAAGAAGAGGAGGGAAAGAAGGAAAAAATATTTTTTTATTAATTTAATAATATTCGTTAGGATTTAACGGAAAAATGGATGGAGAGACTAAAATGAAACGAAATCCAAACTCCATGGTTTAAATTGTCGAATTTGAAAACTCAGAGACCAAAATGAAACCTAACTCAAACCTCAGTGCTAAAAATGTATTTTTCTCTTTTTTTTAGTGTATTTTTTCCTTTATTTTATATTCAATTTCCGTACATTTTTAAAGATCAATTGGTCCTCAACTCTTATCTAGATACTTTTTATGTTGATACTCCTCCTTTTAAATTTTTAAATTTAACCTTTTAACTTTTTATTGAAATTTAGATTTAAATCATAGTTTGGTACTTGTCCAGACTTTCGAATGTTGTATTTTCCCTCTTCAATTTTTAATGTTTTATTTCACTCCTTAATGTGTGTAAGAAATTATTTTAATACCCATCATCATTTTGTTGTTAATGTAATATAGATCTATTTTTCTAAAAAAAAAATAGTGTGTTATCTTTAAATATACTTGTTTACATATACATATATTCTTCAAGTAAATGGACAAAATATATCCTATTTCAAAATTTTATTAAATAGTTTTAAAAAGTAATAGAAAAGGCTAAAATGGTTTATGCAAAGATGATAAAATAAAAAAAGTTGTGGTGATCAAAAAGTTTATAATAGGTCTCTTAACTTTTCTGACAGGTGTAGAAAACATATTTTGGTGTCAAGTTAAAATTTTAATTAATTTTTGAACTTTGTTAATTTTGTTTAAAAGGTTTTTGAGCTCTTAGAGTATTTAATAAATTTCCTAAACTTTCAATTTTATTCTAATAGATTTTTGAACTTTTAATTTTGTGTTTAAGAGATATTTTGACCTATTAGAATTTTTTAAAATTCACAAACCTCTACTAGTCAGACATAGAATTGAAAGGTTAAGGGTGTGTTTGGTTGGTGATTTGAAAACAATGGATTAAATAAAAACAGAGATGTATTTCATGTTTTTCGATATGATATGTGTTTGGTAGCATACTTAGAAATTGGATCCGAATTTAAACAATTGTTCAAAATGTGTTTAATAATATATTTATAAATCATAAGTTATCAACTAATTATTAAGTTGAATATAAACTATTATCAATTATTTAAACATGTTATATGTTATAAATTTTGTATAATTATTATCTTACACTATTATACAATTTATTAAAAAAAAAAAAAAGTTGTTTTCAGAATTTTCACTATTTGAATTACACAATTCGAAAACAATGTTTAAAAACACATCTCCCAAAACACATATTAATTAATTAATGAATTAAGTGAATCTAAAAACATAAAACAAAATTCACATCTGAATTTGTATTACCAACCAAATAGGTGTTAAGATTCTAGTTAGAGACTTAATAGAGACAAAATCAAAGTCTTTTATAGTTCTATTTGTACATTTTTTCAACTTAAGTGATCAATTTTAAACTAGAAGGAGAAATGAGAGTAATTAAAAGGAAACCGACGCATTCCCCGAAACAGAACAAGGCGAGGCTGCCGCCGTCGGGGGCGCTGGGGAAGCAGGCGGTGGCGTTCAGAATATCGGGGGACGCGGCGGCGTTCATAAGCTGCAGGTTTATCGGCGCACAGGACACGCTGTACGACCACATGGGGCGGCACTACTTCAAGAATTGCTACATCGAGGGGTCAGTGGACTTCGTGTTCGGCGACGGGCTGTCCCTCTACGACGGCTGCCACTTGCACGCCATCACCGACAGCTACGGGGCTCTGACCGCCCAGAAGAGGAACAGCCTGCTGGAGGAGACCGGCTTCTCCTTCCTCAACTGCAAGGTTTCCGGGTCGGGCGCTCTCTATTTGGGTCGGGCATGGGGCAGCTTCTCCAGAGTCGTCTTCGCTTATACGTTCATGGACAAAATCATCACTCCCACTGGTTGGTACAACTGGGGCGACAAAAACCGAGAGCTGTACGTATATATATATATATCCTTTTTCTTTCTTTATTGCTATTCTTTTTTTTATATACATAATTTATTGAGAAGAAAGAAGAGAGAAAAAGGTAATTAAAAAGAAAATAATGATGGGGTTTTTGTTGTTTTGGATGTAGGACTGTGTTTTATGGGCAATACAGGTGCTCGGGACCAGGAGCCGATTATGGAGGACGAGTTTCATGGTCCAGAGAACTCACTGAGTCACAAGCCAACCCTTTTCTCTCTCTTGATTTTATTAATGCAAACGACTGGCTTCCAAAGCATACTCGTCTTGCCTTTAAACCTCATTGAAATCGTGACAATTACTAGAGCGAAAAAAAGTTGAAAATGAGAAAGAAATTTTTTTTTTATCTATTCTAATTGTCAACATTTCAACGATGGTGTGTAGGAAGACGTCTATATATTGAGTATGTAGTTAAGTATTGTTCTTTATTATCACTTGCACAAGTTTTTGACAAAACGAGTTTTTTATATTATTTATTTATTTTCAAAT

mRNA sequence

GAAAAATACCATTTAAACTGAGAAGTCACAGATGGGTCTTAAATTTCCATGGCCGTTAATGGCGAAACAGACAATTTGAATACAGACACACAGCTGCAAGATTCCTCCAATGTTGGTCTCTCTCTTCCCCATTTCTGCATTTTTTTCAACTCTTTTAAGCCTCTCATTCTCTCAAACGCTTATCTACACTGCAATCGCAGATATCACGACGCACTTGCTGGTTTAACCATGGCTGCCCAGAGCTGCTTCGAGAAGATGAGGGTGGTGAAGCTTTTGGTTCTGGTGGTGATGATACTGCCGGGCGCCGCCGTTTCTTACGGCCGAAACTGGACGGTCGGCGGCGGCGGGGGAATGAGAAAGGTTGAAGATGAGTACTTGAAGTGGATAAGGCATATGGGTTCTTTCAAGCACTCGCTTTTTCAGAAGACGAAGAACAAGTTCAAGCCTTGTTTGACATTGAAGGTTTCGAAGAAACCCAAGGCGGGAGGTTTCCGGTCGGTGCAGAAGGCTATAAACTCTCTGCCGGTGGTCAACCGGTGCCGGGTCGTCATCCATGTCGCCGCCGGAATCTACAGAGAGAAGGTTGAGATTCCGGCAACGATGAGTTACATATGGGTGGAGGGGGAAGGAGCGGAGAAGACAATCATTGAATGGGGCGACACGGCTGATCATATGGGCGACAATGGCCGTCCCATGGGCACTTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCTTTCTTCATTGCCAGGAACATCACCTTCAAGAACAAGGCGAGGCTGCCGCCGTCGGGGGCGCTGGGGAAGCAGGCGGTGGCGTTCAGAATATCGGGGGACGCGGCGGCGTTCATAAGCTGCAGGTTTATCGGCGCACAGGACACGCTGTACGACCACATGGGGCGGCACTACTTCAAGAATTGCTACATCGAGGGGTCAGTGGACTTCGTGTTCGGCGACGGGCTGTCCCTCTACGACGGCTGCCACTTGCACGCCATCACCGACAGCTACGGGGCTCTGACCGCCCAGAAGAGGAACAGCCTGCTGGAGGAGACCGGCTTCTCCTTCCTCAACTGCAAGGTTTCCGGGTCGGGCGCTCTCTATTTGGGTCGGGCATGGGGCAGCTTCTCCAGAGTCGTCTTCGCTTATACGTTCATGGACAAAATCATCACTCCCACTGGTTGGTACAACTGGGGCGACAAAAACCGAGAGCTGACTGTGTTTTATGGGCAATACAGGTGCTCGGGACCAGGAGCCGATTATGGAGGACGAGTTTCATGGTCCAGAGAACTCACTGAGTCACAAGCCAACCCTTTTCTCTCTCTTGATTTTATTAATGCAAACGACTGGCTTCCAAAGCATACTCGTCTTGCCTTTAAACCTCATTGAAATCGTGACAATTACTAGAGCGAAAAAAAGTTGAAAATGAGAAAGAAATTTTTTTTTTATCTATTCTAATTGTCAACATTTCAACGATGGTGTGTAGGAAGACGTCTATATATTGAGTATGTAGTTAAGTATTGTTCTTTATTATCACTTGCACAAGTTTTTGACAAAACGAGTTTTTTATATTATTTATTTATTTTCAAAT

Coding sequence (CDS)

ATGGCCGTTAATGGCGAAACAGACAATTTGAATACAGACACACAGCTGCAAGATTCCTCCAATGTTGGTCTCTCTCTTCCCCATTTCTGCATTTTTTTCAACTCTTTTAAGCCTCTCATTCTCTCAAACGCTTATCTACACTGCAATCGCAGATATCACGACGCACTTGCTGGTTTAACCATGGCTGCCCAGAGCTGCTTCGAGAAGATGAGGGTGGTGAAGCTTTTGGTTCTGGTGGTGATGATACTGCCGGGCGCCGCCGTTTCTTACGGCCGAAACTGGACGGTCGGCGGCGGCGGGGGAATGAGAAAGGTTGAAGATGAGTACTTGAAGTGGATAAGGCATATGGGTTCTTTCAAGCACTCGCTTTTTCAGAAGACGAAGAACAAGTTCAAGCCTTGTTTGACATTGAAGGTTTCGAAGAAACCCAAGGCGGGAGGTTTCCGGTCGGTGCAGAAGGCTATAAACTCTCTGCCGGTGGTCAACCGGTGCCGGGTCGTCATCCATGTCGCCGCCGGAATCTACAGAGAGAAGGTTGAGATTCCGGCAACGATGAGTTACATATGGGTGGAGGGGGAAGGAGCGGAGAAGACAATCATTGAATGGGGCGACACGGCTGATCATATGGGCGACAATGGCCGTCCCATGGGCACTTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCTTTCTTCATTGCCAGGAACATCACCTTCAAGAACAAGGCGAGGCTGCCGCCGTCGGGGGCGCTGGGGAAGCAGGCGGTGGCGTTCAGAATATCGGGGGACGCGGCGGCGTTCATAAGCTGCAGGTTTATCGGCGCACAGGACACGCTGTACGACCACATGGGGCGGCACTACTTCAAGAATTGCTACATCGAGGGGTCAGTGGACTTCGTGTTCGGCGACGGGCTGTCCCTCTACGACGGCTGCCACTTGCACGCCATCACCGACAGCTACGGGGCTCTGACCGCCCAGAAGAGGAACAGCCTGCTGGAGGAGACCGGCTTCTCCTTCCTCAACTGCAAGGTTTCCGGGTCGGGCGCTCTCTATTTGGGTCGGGCATGGGGCAGCTTCTCCAGAGTCGTCTTCGCTTATACGTTCATGGACAAAATCATCACTCCCACTGGTTGGTACAACTGGGGCGACAAAAACCGAGAGCTGACTGTGTTTTATGGGCAATACAGGTGCTCGGGACCAGGAGCCGATTATGGAGGACGAGTTTCATGGTCCAGAGAACTCACTGAGTCACAAGCCAACCCTTTTCTCTCTCTTGATTTTATTAATGCAAACGACTGGCTTCCAAAGCATACTCGTCTTGCCTTTAAACCTCATTGA

Protein sequence

MAVNGETDNLNTDTQLQDSSNVGLSLPHFCIFFNSFKPLILSNAYLHCNRRYHDALAGLTMAAQSCFEKMRVVKLLVLVVMILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKTKNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYDGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFINANDWLPKHTRLAFKPH
Homology
BLAST of Lcy11g009010 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 2.8e-132
Identity = 212/328 (64.63%), Postives = 265/328 (80.79%), Query Frame = 0

Query: 106 EDEYLKWIRHMGSFKHSLFQKTKNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCR 165
           EDE++KW+R +GS KHS+F+  KNK  P  TL V KK   G F  +Q AI+SLP++N  R
Sbjct: 54  EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113

Query: 166 VVIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFA 225
           VVI V AG+Y+EKV IP   ++I +EGEGAEKT +EWGDTA      G PMGT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173

Query: 226 VNSPFFIARNITFKNKARLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRH 285
           VNSPFF+A+NITF+N   +P  GA+GKQAVA R+S D AAF  CR +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233

Query: 286 YFKNCYIEGSVDFVFGDGLSLYDGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSG 345
           Y+K+CYIEGSVDF+FG+ LSLY+GCH+HAI D  GA+TAQ R+S+LE+TGFSF+ CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293

Query: 346 SGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGG 405
           +G LYLGRAWG FSRVVFAYT+MD II P GWYNWGD +RE+TVFYGQY+C+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353

Query: 406 RVSWSRELTESQANPFLSLDFINANDWL 434
           RV+W+RELT+ +A PFLSL FI+ ++W+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of Lcy11g009010 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 9.5e-80
Identity = 141/287 (49.13%), Postives = 188/287 (65.51%), Query Frame = 0

Query: 148 FRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 207
           FRSVQ A++S+P  N   + I +A G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 208 HMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVAFRISGDAAAFI 267
            +G NG+ + T+ +A+  V + +F ARNI+F N A  P  G  G QAVAFRISGD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 268 SCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYDGCHLHAITDSYGALTAQKR 327
            C F GAQDTL D  GRHYFK CYIEGS+DF+FG+G S+Y  C LH+I   +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 328 NSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDK-NRE 387
               E+TGF+F+ C+V+G+G LY+GRA G +SR+V+AYT+ D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 388 LTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFINANDWL 434
            T F+G Y C GPGA     VSW+R L    A+PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of Lcy11g009010 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 4.9e-76
Identity = 144/319 (45.14%), Postives = 206/319 (64.58%), Query Frame = 0

Query: 126 KTKNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATM 185
           + ++++K  L L V      G F +VQ AI+ +P ++  + +I V +G YREKV +    
Sbjct: 80  RLRHQYKTSLVLTVDLH-GCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139

Query: 186 SYIWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLP 245
           + + ++G G + T IEW DTA   G+      +F+   FA N   F A NI+FKN A  P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199

Query: 246 PSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLS 305
             G    QAVA RI GD AAF  C F GAQDTL D  GRH+FK C+I+GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259

Query: 306 LYDGCHLHAITDS-----YGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSR 365
           LY  C +++I         G++TAQ R S  E++GFSF+NCK+ GSG + LGRAWG+++ 
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319

Query: 366 VVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANP 425
           VVF+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++LT+S+A+ 
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379

Query: 426 FLSLDFINANDWLPKHTRL 440
           F+ + FI+ ++WL +HT +
Sbjct: 380 FIDVSFIDGDEWL-RHTNI 390

BLAST of Lcy11g009010 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 4.6e-74
Identity = 136/297 (45.79%), Postives = 187/297 (62.96%), Query Frame = 0

Query: 139 VSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEG--AE 198
           V  K   G   +VQ A++ +P  N  RV I +  GIYREKV +P +  YI   G    A 
Sbjct: 83  VVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAG 142

Query: 199 KTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVA 258
            T+I W D A  +G +G+ +GT+ +A+ ++ S FF A  ITF+N   +  +G  G+QAVA
Sbjct: 143 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVA 202

Query: 259 FRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYDGCHLHAIT 318
            RI GD A F   R +G+QDTL+D  G HYF  CYI+G+VDF+FG+  SLY  C +H+  
Sbjct: 203 LRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTA 262

Query: 319 DSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTG 378
             YGA+ A  R+S  E+TGFSF+NC +SG+G +YLGRAWG++SR V++  F+  IITP G
Sbjct: 263 KRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVG 322

Query: 379 WYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFINANDWL 434
           W +W    R+  V +G+Y C G GA+ GGRV WS+ LT  +  PFL  +FI  + WL
Sbjct: 323 WSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

BLAST of Lcy11g009010 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.5e-72
Identity = 141/317 (44.48%), Postives = 190/317 (59.94%), Query Frame = 0

Query: 131 FKPCLTLKVSKKPKAGGFRSVQKAINSLP--VVNRCRVVIHVAAGIYREKVEIPATMSYI 190
           F   + ++V +  K G F  +Q+AI S+P  + N     I V  GIYREKV IPA   YI
Sbjct: 45  FSTAILIRVDQSGK-GDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 104

Query: 191 WVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSG 250
            + G  A  T + W D  D             S T  + +  F+ R +T +NK      G
Sbjct: 105 TLSGTQASNTFLIWSDGED----------ILESPTLTIFASDFVCRFLTIQNK-----FG 164

Query: 251 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYD 310
             G+ AVA R++ D AAF  C     QDTL D  G HYFKNCYIEG+ DF+ G   SLY+
Sbjct: 165 TAGR-AVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYE 224

Query: 311 GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM 370
            CHLH+++ + G++TAQ R S  E++GF+FL CK++GSG+ +LGR WG++SRVVFAY+F 
Sbjct: 225 RCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFF 284

Query: 371 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFIN 430
             ++ P GW  WGD  +E TV+YG+Y+C GPGAD   RV WS++L++ +A  FLS DFI 
Sbjct: 285 SNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIG 344

Query: 431 ANDWL---PKHTRLAFK 443
             DWL   P H + A K
Sbjct: 345 GKDWLRPAPSHFKNAPK 344

BLAST of Lcy11g009010 vs. ExPASy TrEMBL
Match: A0A6J1J245 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.3e-201
Identity = 342/379 (90.24%), Postives = 359/379 (94.72%), Query Frame = 0

Query: 70  MRVVKLLVLVVMI--LPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKT 129
           MR++   VLVVMI  LPGAAVSYG+NWTV GGGGMR+VEDEYLKWIRHMGSFKHSLFQ T
Sbjct: 1   MRMLNFFVLVVMIMLLPGAAVSYGQNWTV-GGGGMRRVEDEYLKWIRHMGSFKHSLFQNT 60

Query: 130 KNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSY 189
           KNKFKPCLTL+VSKKPK GGFRSVQKAINSLP++NRCRVVIHVAAGIYREKVEIPA MSY
Sbjct: 61  KNKFKPCLTLRVSKKPKPGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSY 120

Query: 190 IWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPS 249
           IWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SATFAVNSPFFIARNITFKNKAR PPS
Sbjct: 121 IWVEGEGAEKTVIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPS 180

Query: 250 GALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLY 309
           GALGKQAVAFRI+GDAAAFISCRFIGAQDTLYDH+GRHYFK+CYIEGSVDFVFGDGLS+Y
Sbjct: 181 GALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIY 240

Query: 310 DGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 369
           +GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF
Sbjct: 241 EGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 300

Query: 370 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFI 429
           MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTE +ANPF+SLDFI
Sbjct: 301 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTEPEANPFVSLDFI 360

Query: 430 NANDWLPKHTRLAFK--PH 445
            A +WLPK T LA K  PH
Sbjct: 361 GAKEWLPKRTHLAIKSPPH 378

BLAST of Lcy11g009010 vs. ExPASy TrEMBL
Match: A0A6J1FW15 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 7.3e-200
Identity = 341/378 (90.21%), Postives = 358/378 (94.71%), Query Frame = 0

Query: 70  MRVVKLLVLVV-MILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKTK 129
           MR++   VLVV M+L GAAVS+G+NWTV GGG MRKVEDEYLKWIRHMGSFKHSLFQ TK
Sbjct: 1   MRMLNFFVLVVMMLLSGAAVSHGQNWTV-GGGRMRKVEDEYLKWIRHMGSFKHSLFQNTK 60

Query: 130 NKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYI 189
           NKFKPCLTL+VSKKPKAGGFRSVQKAINSLP++NRCRVVIHVAAGIYREKVEIPA MSYI
Sbjct: 61  NKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSYI 120

Query: 190 WVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSG 249
           WVEGEGAEKTIIEW DTADHMGDNGRP+GTF SATFAVNSPFFIARNITFKNKAR PPSG
Sbjct: 121 WVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPSG 180

Query: 250 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYD 309
           ALGKQAVAFRI+GDAAAFISCRFIGAQDTLYDH+GRHYFK+CYIEGSVDFVFGDGLS+Y+
Sbjct: 181 ALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIYE 240

Query: 310 GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM 369
           GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM
Sbjct: 241 GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM 300

Query: 370 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFIN 429
           DKIITPTGWYNWGDKN ELTVFYGQYRCSGPGADYGGRVSWSRELTES+ANPF+SLDFI 
Sbjct: 301 DKIITPTGWYNWGDKNLELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDFIG 360

Query: 430 ANDWLPKHTRLAFK--PH 445
           A +WLPK T LA K  PH
Sbjct: 361 AKEWLPKRTHLAIKSPPH 377

BLAST of Lcy11g009010 vs. ExPASy TrEMBL
Match: A0A1S3CNF2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.4e-195
Identity = 330/371 (88.95%), Postives = 348/371 (93.80%), Query Frame = 0

Query: 75  LLVLVVMILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKTKNKFKPC 134
           L +L+ +ILP AA    +NWT GGG GMR V+DEYLKWIRHMGSFKHSLFQ TKNKFKPC
Sbjct: 9   LFLLIAIILPAAA----QNWTAGGGRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKPC 68

Query: 135 LTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEG 194
           LTLKVSK  K GGFRS+QKA+NSLP++NRCRV IHVAAGIYREKVEIPATMSYIWVEGEG
Sbjct: 69  LTLKVSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEG 128

Query: 195 AEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQA 254
           AEKTIIEWGDTADHMG+NGRPMGTFASATFAVNSPFFIA NITFKNKARLPPSGALGKQA
Sbjct: 129 AEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQA 188

Query: 255 VAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYDGCHLHA 314
           VAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFK+CYIEGSVDFVFGDGLS+YD CHLHA
Sbjct: 189 VAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHA 248

Query: 315 ITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITP 374
           IT+SYGALTAQKRNS+LEETGFSFL+CKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITP
Sbjct: 249 ITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITP 308

Query: 375 TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFINANDWLP 434
           TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELT+S+ANPFLSLDFINA  WLP
Sbjct: 309 TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAKQWLP 368

Query: 435 K-HTRLAFKPH 445
             HT L+ KPH
Sbjct: 369 NYHTHLSHKPH 375

BLAST of Lcy11g009010 vs. ExPASy TrEMBL
Match: A0A0A0KWM1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.6e-194
Identity = 329/370 (88.92%), Postives = 347/370 (93.78%), Query Frame = 0

Query: 77  VLVVMILPGAAVSYGRNWTVGGGGGMRK-VEDEYLKWIRHMGSFKHSLFQKTKNKFKPCL 136
           +L+ +ILP AA    +NWT GGG GMR  V+DEY KWIRHMGSFKHSLFQ TKNKFKPCL
Sbjct: 12  LLIAIILPAAA----QNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTKNKFKPCL 71

Query: 137 TLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEGA 196
           TLKVSK  KAGGFRS+QKA+NSLP++NRCRV IHVAAGIYREKVEIPATMSYIWVEGEGA
Sbjct: 72  TLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGA 131

Query: 197 EKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAV 256
           EKTIIEWGDTADHMG+NGRPMGTFASATFAVNSPFFIA NITFKNKARLPPSGALGKQAV
Sbjct: 132 EKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAV 191

Query: 257 AFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYDGCHLHAI 316
           AFRISGDAAAFISCRFIGAQDTLYDHMGRHYFK+CYIEGSVDFVFGDGLS+YD CHLHAI
Sbjct: 192 AFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAI 251

Query: 317 TDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPT 376
           T+SYGALTAQKRNS+LEETGFSFL+CKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPT
Sbjct: 252 TNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPT 311

Query: 377 GWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFINANDWLPK 436
           GWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELT+S+ANPFLSLDFINAN WLP 
Sbjct: 312 GWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWLPN 371

Query: 437 -HTRLAFKPH 445
            HT L+ KPH
Sbjct: 372 YHTHLSLKPH 377

BLAST of Lcy11g009010 vs. ExPASy TrEMBL
Match: A0A5A7TMQ1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 PE=4 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 4.2e-187
Identity = 321/379 (84.70%), Postives = 341/379 (89.97%), Query Frame = 0

Query: 75  LLVLVVMILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKTKNKFKPC 134
           L +L+ +ILP AA    +NWT GGG GMR V+DEYLKWIRHMGSFKHSLFQ TKNKFKPC
Sbjct: 9   LFLLIAIILPAAA----QNWTAGGGRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKPC 68

Query: 135 LTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEG 194
           LTLKVSK  K GGFRS+QKA+NSLP++NRCRV IHVAAGIYREKVEIPATMSYIWVEGEG
Sbjct: 69  LTLKVSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEG 128

Query: 195 AEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFK--------NKARLPP 254
           AEKTIIEWGDTADHMG+NGRPMGTFASATFAVNSPFFIA NITFK          + LP 
Sbjct: 129 AEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKVLTFSSFFLSSLLPL 188

Query: 255 SGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSL 314
             +LGKQAVAFRISGDAA FISCRFIGAQDTLYDHMGRHYFK+CYIEGSVDFVFGDGLS+
Sbjct: 189 KFSLGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSI 248

Query: 315 YDGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 374
           YD CHLHAIT+SYGALTAQKRNS+LEETGFSFL+CKVSGSGALYLGRAWGSFSRVVFAYT
Sbjct: 249 YDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYT 308

Query: 375 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDF 434
           FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELT+S+ANPFLSLDF
Sbjct: 309 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDF 368

Query: 435 INANDWLPK-HTRLAFKPH 445
           INA  WLP  HT L+ KPH
Sbjct: 369 INAKQWLPNYHTHLSHKPH 383

BLAST of Lcy11g009010 vs. NCBI nr
Match: XP_023527781.1 (probable pectinesterase 53 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 725.3 bits (1871), Expect = 3.1e-205
Identity = 363/447 (81.21%), Postives = 383/447 (85.68%), Query Frame = 0

Query: 1   MAVNGETDNLNTDTQLQDSSNVGLSLPHFCIFFNSFKPLILSNAYLHCNRRYHDALAGLT 60
           MAVNG TDNLNTDTQLQDS +V                                      
Sbjct: 1   MAVNGNTDNLNTDTQLQDSPDV-------------------------------------- 60

Query: 61  MAAQSCFEKMRVVKLLVLVV-MILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSF 120
           +AA    +KMRV+   VLVV M+L GAAVS+G+NWTV GGG MRKVEDEYLKWIRHMGSF
Sbjct: 61  VAA----DKMRVLNFFVLVVMMLLSGAAVSHGQNWTV-GGGRMRKVEDEYLKWIRHMGSF 120

Query: 121 KHSLFQKTKNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKV 180
           KHSLFQ TKNKFKPCLTL+VSKKPKAGGFRSVQKAINSLP++NRCRVVIHVAAGIYREKV
Sbjct: 121 KHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKV 180

Query: 181 EIPATMSYIWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFK 240
           EIPA MSYIWVEGEGAEKTIIEW DTADHMGDNGRP+GTF SATFAVNSPFF+ARNITFK
Sbjct: 181 EIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFVARNITFK 240

Query: 241 NKARLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFV 300
           NKAR PPSGALGKQAVAFRI+GDAAAFISCRFIGAQDTLYDH+GRHYFK+CYIEGSVDFV
Sbjct: 241 NKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFV 300

Query: 301 FGDGLSLYDGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFS 360
           FGDGLS+Y+GCHLHAITDSYGALTAQKRNSLLEETGFSFL CKVSGSGALYLGRAWGSFS
Sbjct: 301 FGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLKCKVSGSGALYLGRAWGSFS 360

Query: 361 RVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQAN 420
           RVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTES+AN
Sbjct: 361 RVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEAN 404

Query: 421 PFLSLDFINANDWLPKHTRLAFK--PH 445
           PF+SLDFI A +WLPK T LA K  PH
Sbjct: 421 PFVSLDFIGAKEWLPKRTHLAIKSPPH 404

BLAST of Lcy11g009010 vs. NCBI nr
Match: KAG6600598.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714.1 bits (1842), Expect = 7.2e-202
Identity = 345/380 (90.79%), Postives = 362/380 (95.26%), Query Frame = 0

Query: 68  EKMRVVKLLVLVV-MILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQK 127
           +KMR++  LVLVV M+L GAAVS+G+NWTV GGG MRKVEDEYLKWIRHMGSFKHSLFQ 
Sbjct: 36  DKMRMLNFLVLVVTMLLSGAAVSHGQNWTV-GGGRMRKVEDEYLKWIRHMGSFKHSLFQN 95

Query: 128 TKNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMS 187
           TKNKFKPCLTL+VSKKPKAGGFRSVQKAINSLP+VNRCRVVIHVAAGIYREKVEIPA MS
Sbjct: 96  TKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNRCRVVIHVAAGIYREKVEIPAMMS 155

Query: 188 YIWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPP 247
           YIWVEGEGAEKTIIEW DTADHMGDNGRP+GTF SATFAVNSPFFIARNITFKNKAR PP
Sbjct: 156 YIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPP 215

Query: 248 SGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSL 307
           SGALGKQAVAFRI+GDAAAFISCRFIGAQDTLYDH+GRHYFK+CYIEGSVDFVFGDGLS+
Sbjct: 216 SGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSI 275

Query: 308 YDGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 367
           Y+GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT
Sbjct: 276 YEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 335

Query: 368 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDF 427
           FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTES+ANPF+SLDF
Sbjct: 336 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDF 395

Query: 428 INANDWLPKHTRLAFK--PH 445
           I A +WLPK T LA K  PH
Sbjct: 396 IGAKEWLPKRTHLAIKSPPH 414

BLAST of Lcy11g009010 vs. NCBI nr
Match: XP_022981448.1 (probable pectinesterase 53 [Cucurbita maxima])

HSP 1 Score: 712.2 bits (1837), Expect = 2.8e-201
Identity = 342/379 (90.24%), Postives = 359/379 (94.72%), Query Frame = 0

Query: 70  MRVVKLLVLVVMI--LPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKT 129
           MR++   VLVVMI  LPGAAVSYG+NWTV GGGGMR+VEDEYLKWIRHMGSFKHSLFQ T
Sbjct: 1   MRMLNFFVLVVMIMLLPGAAVSYGQNWTV-GGGGMRRVEDEYLKWIRHMGSFKHSLFQNT 60

Query: 130 KNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSY 189
           KNKFKPCLTL+VSKKPK GGFRSVQKAINSLP++NRCRVVIHVAAGIYREKVEIPA MSY
Sbjct: 61  KNKFKPCLTLRVSKKPKPGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSY 120

Query: 190 IWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPS 249
           IWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SATFAVNSPFFIARNITFKNKAR PPS
Sbjct: 121 IWVEGEGAEKTVIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPS 180

Query: 250 GALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLY 309
           GALGKQAVAFRI+GDAAAFISCRFIGAQDTLYDH+GRHYFK+CYIEGSVDFVFGDGLS+Y
Sbjct: 181 GALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIY 240

Query: 310 DGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 369
           +GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF
Sbjct: 241 EGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 300

Query: 370 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFI 429
           MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTE +ANPF+SLDFI
Sbjct: 301 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTEPEANPFVSLDFI 360

Query: 430 NANDWLPKHTRLAFK--PH 445
            A +WLPK T LA K  PH
Sbjct: 361 GAKEWLPKRTHLAIKSPPH 378

BLAST of Lcy11g009010 vs. NCBI nr
Match: KAG7031236.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 709.5 bits (1830), Expect = 1.8e-200
Identity = 343/378 (90.74%), Postives = 359/378 (94.97%), Query Frame = 0

Query: 70  MRVVKLLVLVV-MILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKTK 129
           MR++   VLVV M+L GAAVS+G+NWTV GGG MRKVEDEYLKWIRHMGSFKHSLFQ TK
Sbjct: 1   MRMLNFFVLVVTMLLSGAAVSHGQNWTV-GGGRMRKVEDEYLKWIRHMGSFKHSLFQNTK 60

Query: 130 NKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYI 189
           NKFKPCLTL+VSKKPKAGGFRSVQKAINSLP+VNRCRVVIHVAAGIYREKVEIPA MSYI
Sbjct: 61  NKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNRCRVVIHVAAGIYREKVEIPAMMSYI 120

Query: 190 WVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSG 249
           WVEGEGAEKTIIEW DTADHMGDNGRP+GTF SATFAVNSPFFIARNITFKNKAR PPSG
Sbjct: 121 WVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPSG 180

Query: 250 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYD 309
           ALGKQAVAFRI+GDAAAFISCRFIGAQDTLYDH+GRHYFK+CYIEGSVDFVFGDGLS+Y+
Sbjct: 181 ALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIYE 240

Query: 310 GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM 369
           GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM
Sbjct: 241 GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM 300

Query: 370 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFIN 429
           DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTES+ANPF+SLDFI 
Sbjct: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDFIG 360

Query: 430 ANDWLPKHTRLAFK--PH 445
           A +WLPK T LA K  PH
Sbjct: 361 AKEWLPKRTHLAIKSPPH 377

BLAST of Lcy11g009010 vs. NCBI nr
Match: XP_023527790.1 (probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 707.6 bits (1825), Expect = 6.8e-200
Identity = 341/378 (90.21%), Postives = 358/378 (94.71%), Query Frame = 0

Query: 70  MRVVKLLVLVV-MILPGAAVSYGRNWTVGGGGGMRKVEDEYLKWIRHMGSFKHSLFQKTK 129
           MRV+   VLVV M+L GAAVS+G+NWTV GGG MRKVEDEYLKWIRHMGSFKHSLFQ TK
Sbjct: 1   MRVLNFFVLVVMMLLSGAAVSHGQNWTV-GGGRMRKVEDEYLKWIRHMGSFKHSLFQNTK 60

Query: 130 NKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYI 189
           NKFKPCLTL+VSKKPKAGGFRSVQKAINSLP++NRCRVVIHVAAGIYREKVEIPA MSYI
Sbjct: 61  NKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSYI 120

Query: 190 WVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSG 249
           WVEGEGAEKTIIEW DTADHMGDNGRP+GTF SATFAVNSPFF+ARNITFKNKAR PPSG
Sbjct: 121 WVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFVARNITFKNKARAPPSG 180

Query: 250 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYD 309
           ALGKQAVAFRI+GDAAAFISCRFIGAQDTLYDH+GRHYFK+CYIEGSVDFVFGDGLS+Y+
Sbjct: 181 ALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIYE 240

Query: 310 GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM 369
           GCHLHAITDSYGALTAQKRNSLLEETGFSFL CKVSGSGALYLGRAWGSFSRVVFAYTFM
Sbjct: 241 GCHLHAITDSYGALTAQKRNSLLEETGFSFLKCKVSGSGALYLGRAWGSFSRVVFAYTFM 300

Query: 370 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFIN 429
           DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTES+ANPF+SLDFI 
Sbjct: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDFIG 360

Query: 430 ANDWLPKHTRLAFK--PH 445
           A +WLPK T LA K  PH
Sbjct: 361 AKEWLPKRTHLAIKSPPH 377

BLAST of Lcy11g009010 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 473.4 bits (1217), Expect = 2.0e-133
Identity = 212/328 (64.63%), Postives = 265/328 (80.79%), Query Frame = 0

Query: 106 EDEYLKWIRHMGSFKHSLFQKTKNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCR 165
           EDE++KW+R +GS KHS+F+  KNK  P  TL V KK   G F  +Q AI+SLP++N  R
Sbjct: 54  EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113

Query: 166 VVIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFA 225
           VVI V AG+Y+EKV IP   ++I +EGEGAEKT +EWGDTA      G PMGT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173

Query: 226 VNSPFFIARNITFKNKARLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRH 285
           VNSPFF+A+NITF+N   +P  GA+GKQAVA R+S D AAF  CR +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233

Query: 286 YFKNCYIEGSVDFVFGDGLSLYDGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSG 345
           Y+K+CYIEGSVDF+FG+ LSLY+GCH+HAI D  GA+TAQ R+S+LE+TGFSF+ CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293

Query: 346 SGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGG 405
           +G LYLGRAWG FSRVVFAYT+MD II P GWYNWGD +RE+TVFYGQY+C+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353

Query: 406 RVSWSRELTESQANPFLSLDFINANDWL 434
           RV+W+RELT+ +A PFLSL FI+ ++W+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of Lcy11g009010 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 6.8e-81
Identity = 141/287 (49.13%), Postives = 188/287 (65.51%), Query Frame = 0

Query: 148 FRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 207
           FRSVQ A++S+P  N   + I +A G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 208 HMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVAFRISGDAAAFI 267
            +G NG+ + T+ +A+  V + +F ARNI+F N A  P  G  G QAVAFRISGD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 268 SCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYDGCHLHAITDSYGALTAQKR 327
            C F GAQDTL D  GRHYFK CYIEGS+DF+FG+G S+Y  C LH+I   +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 328 NSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDK-NRE 387
               E+TGF+F+ C+V+G+G LY+GRA G +SR+V+AYT+ D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 388 LTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFINANDWL 434
            T F+G Y C GPGA     VSW+R L    A+PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of Lcy11g009010 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 3.5e-77
Identity = 144/319 (45.14%), Postives = 206/319 (64.58%), Query Frame = 0

Query: 126 KTKNKFKPCLTLKVSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATM 185
           + ++++K  L L V      G F +VQ AI+ +P ++  + +I V +G YREKV +    
Sbjct: 80  RLRHQYKTSLVLTVDLH-GCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139

Query: 186 SYIWVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLP 245
           + + ++G G + T IEW DTA   G+      +F+   FA N   F A NI+FKN A  P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199

Query: 246 PSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLS 305
             G    QAVA RI GD AAF  C F GAQDTL D  GRH+FK C+I+GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259

Query: 306 LYDGCHLHAITDS-----YGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSR 365
           LY  C +++I         G++TAQ R S  E++GFSF+NCK+ GSG + LGRAWG+++ 
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319

Query: 366 VVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANP 425
           VVF+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++LT+S+A+ 
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379

Query: 426 FLSLDFINANDWLPKHTRL 440
           F+ + FI+ ++WL +HT +
Sbjct: 380 FIDVSFIDGDEWL-RHTNI 390

BLAST of Lcy11g009010 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 3.3e-75
Identity = 136/297 (45.79%), Postives = 187/297 (62.96%), Query Frame = 0

Query: 139 VSKKPKAGGFRSVQKAINSLPVVNRCRVVIHVAAGIYREKVEIPATMSYIWVEGEG--AE 198
           V  K   G   +VQ A++ +P  N  RV I +  GIYREKV +P +  YI   G    A 
Sbjct: 83  VVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAG 142

Query: 199 KTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVA 258
            T+I W D A  +G +G+ +GT+ +A+ ++ S FF A  ITF+N   +  +G  G+QAVA
Sbjct: 143 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVA 202

Query: 259 FRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYDGCHLHAIT 318
            RI GD A F   R +G+QDTL+D  G HYF  CYI+G+VDF+FG+  SLY  C +H+  
Sbjct: 203 LRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTA 262

Query: 319 DSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTG 378
             YGA+ A  R+S  E+TGFSF+NC +SG+G +YLGRAWG++SR V++  F+  IITP G
Sbjct: 263 KRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVG 322

Query: 379 WYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFINANDWL 434
           W +W    R+  V +G+Y C G GA+ GGRV WS+ LT  +  PFL  +FI  + WL
Sbjct: 323 WSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

BLAST of Lcy11g009010 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 274.2 bits (700), Expect = 1.8e-73
Identity = 141/317 (44.48%), Postives = 190/317 (59.94%), Query Frame = 0

Query: 131 FKPCLTLKVSKKPKAGGFRSVQKAINSLP--VVNRCRVVIHVAAGIYREKVEIPATMSYI 190
           F   + ++V +  K G F  +Q+AI S+P  + N     I V  GIYREKV IPA   YI
Sbjct: 45  FSTAILIRVDQSGK-GDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 104

Query: 191 WVEGEGAEKTIIEWGDTADHMGDNGRPMGTFASATFAVNSPFFIARNITFKNKARLPPSG 250
            + G  A  T + W D  D             S T  + +  F+ R +T +NK      G
Sbjct: 105 TLSGTQASNTFLIWSDGED----------ILESPTLTIFASDFVCRFLTIQNK-----FG 164

Query: 251 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKNCYIEGSVDFVFGDGLSLYD 310
             G+ AVA R++ D AAF  C     QDTL D  G HYFKNCYIEG+ DF+ G   SLY+
Sbjct: 165 TAGR-AVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYE 224

Query: 311 GCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFM 370
            CHLH+++ + G++TAQ R S  E++GF+FL CK++GSG+ +LGR WG++SRVVFAY+F 
Sbjct: 225 RCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFF 284

Query: 371 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESQANPFLSLDFIN 430
             ++ P GW  WGD  +E TV+YG+Y+C GPGAD   RV WS++L++ +A  FLS DFI 
Sbjct: 285 SNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIG 344

Query: 431 ANDWL---PKHTRLAFK 443
             DWL   P H + A K
Sbjct: 345 GKDWLRPAPSHFKNAPK 344

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYZ32.8e-13264.63Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q8LPF39.5e-8049.13Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q9ZQA34.9e-7645.14Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q9FM794.6e-7445.79Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Q9SIJ92.5e-7244.48Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J2451.3e-20190.24Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1[more]
A0A6J1FW157.3e-20090.21Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1[more]
A0A1S3CNF21.4e-19588.95Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1[more]
A0A0A0KWM11.6e-19488.92Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1[more]
A0A5A7TMQ14.2e-18784.70Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 ... [more]
Match NameE-valueIdentityDescription
XP_023527781.13.1e-20581.21probable pectinesterase 53 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6600598.17.2e-20290.79putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022981448.12.8e-20190.24probable pectinesterase 53 [Cucurbita maxima][more]
KAG7031236.11.8e-20090.74putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023527790.16.8e-20090.21probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G19730.12.0e-13364.63Pectin lyase-like superfamily protein [more]
AT5G47500.16.8e-8149.13Pectin lyase-like superfamily protein [more]
AT2G36710.13.5e-7745.14Pectin lyase-like superfamily protein [more]
AT5G55590.13.3e-7545.79Pectin lyase-like superfamily protein [more]
AT2G21610.11.8e-7344.48pectinesterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 136..441
e-value: 2.0E-100
score: 338.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 144..429
e-value: 1.5E-71
score: 240.6
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 102..433
NoneNo IPR availablePANTHERPTHR31321:SF103PECTINESTERASEcoord: 102..433
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 292..301
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 136..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy11g009010.1Lcy11g009010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity