Lcy11g005030 (gene) Sponge gourd (P93075) v1

Overview
NameLcy11g005030
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr11: 4820011 .. 4821986 (-)
RNA-Seq ExpressionLcy11g005030
SyntenyLcy11g005030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTAACCATGGTTAGGTCAAAGAAAAAGAAAAATAAAAAAAAGAAATTCTTACACCACACGTGTGGGCCGAAGTCAGATAAGGAGCAGAAGAACAGACTACAGAGGCAGTCTTTGTTTCTCCTTTATCTCTCTCTTTTACCTGTTGGGTATGTCTCTCTGTTCCATTCAATTTTAGAGAGAAGAACAAACACAGAGATAAAGAGAGAGAGAGTCTAAAAAAAGCTAACACTGAAAATTTGCAGAGCGCTCAGATTTGTTAGTATTAGTATTTGTAGTTTCTGTGTGTTTGTGTACAATCGTCCCCTCCCCCACCTGTTTGACAAAATTTCTCTTTCAAAAAAATTCAGACCAGAGAGAGTTTATAGAAAAACATGCCTGGAAGTGTTGTTGTTGAACCCTTGGATTTGAGCGTACACATTCCATACCATTTCAGGTGTCCGATTTCTCTCGAGCTCATGCGCGACCCGGTTACGGTCAGCACTGGTCAGACCTACGACCGCTCCAGTATTGAGTCATGGGTGGCCACCGGCAACACCACCTGTCCGGTCACTAGGGCTCCGCTCACCGACTTCACGCTCATCCCCAACCATACGCTGCGACGCCTCATTCAGGATTGGTGCGTTGCAAACCGCTCTTATGGCGTCGAGCGGATTCCGACTCCGAAGCAACCGGCCGATCCGTCTTTGGTTCGGTCTCTGCTTGCTCAAGCTTCGTCGAAATCGAGTGGTTCGAGTTCCAGAATCTCGGCTCTGCGGCGGTTGAAAGGACTCGCTCGTGACTCGGATAAGAATCGGTCGGTGATTTCCTCGCTCAACGCTCGTGAAATTCTTCTTGATATTGTGTTTTCGAATTTGGATTTGGATTTGGATTTGGATTTGGATTCGGATTCGGTTTCCCCTGATTTGAGCCGCGAATCGCTCGCGTTGCTTGTGATGTTGCCGCTCACGGAATCTGAGTGCGTTCTCGTTGCGTCTGATCCGCAACGGATTGGTTACCTGTCACATCTTCTGTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCTGCGTTGATTGAAATTGTGATTGCTGGAACTCGAGCTTCGGAGCTTCGAGGTCAAATTTGCGCTATCGATGAATTGTTCGAAGGCGTGGTTGAGATTCTCAGAGATCCAACGGCGTATCCGCGAGCGCTCAAGGTCGGAGTTAAGGCCTTGTTCGCTCTCTGTCTGGTGAAACAGACCAGGCACAAGGCGGTTTCCGCAGGCGCCGCCGAGATTCTCGTCGACAGATTCCCCGATTTGGAGAAATACGACGCGGAACGAGCCCTAGCGACGATCGAACTCATATGCAGAATTCCGGCCGGTTGCGACGCGTTTGCCGCTCACGCGCTCACCGTTCCTCTGTTAGTGAAAGTCATTCTGAAGATCTCGGATAGAGCGACGGAATCGGCGGTGGGAGCTCTGGTGTCGCTGTGCTCGGCGTCGGAGGAGAACCGGCGGGAGGCGGTGGCGGCGGGGATTTTTACACAGTTGTTGTTGCTGGTGCAAAGCGATTGTACGGACCGCGTTAAGCGGAAAACGCAGGTGCTGCTGAAGCTTCTTCGGGATTCATGGCCGCAAGATTCCGTCGGAAATTCCGACGACTATGCTTGCAGTGAAATTGTGGTGCCGTTTTGTTAATTGTGGAGTCTCACATTTTGCATCTCTGATCTCCAGTTTGTTCTGTAAGTTGAAGATTCATCCGGGAAGAAAGAAGAAGGAAGAATTGTGAACTAGAGACGAACAGACAAAGTTGCAGTAGTTTTCTGTATGTAATTTTTGTACGTAAATTTTTTTGTTCGATAGCTTTCAGAAAAAAGAAAAATCCAAAAAAAAAAGAAAAAAAGAAAGGAATTATTTTCGTCTATTGTTAAGTTTTTATTTGAAAGATTTTTGACATTTGTAACAGGATTTCCTCTTAGTGATATTTTAACATCATTAATTTTCTCTTTTA

mRNA sequence

CCTTAACCATGGTTAGGTCAAAGAAAAAGAAAAATAAAAAAAAGAAATTCTTACACCACACGTGTGGGCCGAAGTCAGATAAGGAGCAGAAGAACAGACTACAGAGGCAGTCTTTGTTTCTCCTTTATCTCTCTCTTTTACCTGTTGGGTATGTCTCTCTGTTCCATTCAATTTTAGAGAGAAGAACAAACACAGAGATAAAGAGAGAGAGAGTCTAAAAAAAGCTAACACTGAAAATTTGCAGAGCGCTCAGATTTGTTAGTATTAGTATTTGTAGTTTCTGTGTGTTTGTGTACAATCGTCCCCTCCCCCACCTGTTTGACAAAATTTCTCTTTCAAAAAAATTCAGACCAGAGAGAGTTTATAGAAAAACATGCCTGGAAGTGTTGTTGTTGAACCCTTGGATTTGAGCGTACACATTCCATACCATTTCAGGTGTCCGATTTCTCTCGAGCTCATGCGCGACCCGGTTACGGTCAGCACTGGTCAGACCTACGACCGCTCCAGTATTGAGTCATGGGTGGCCACCGGCAACACCACCTGTCCGGTCACTAGGGCTCCGCTCACCGACTTCACGCTCATCCCCAACCATACGCTGCGACGCCTCATTCAGGATTGGTGCGTTGCAAACCGCTCTTATGGCGTCGAGCGGATTCCGACTCCGAAGCAACCGGCCGATCCGTCTTTGGTTCGGTCTCTGCTTGCTCAAGCTTCGTCGAAATCGAGTGGTTCGAGTTCCAGAATCTCGGCTCTGCGGCGGTTGAAAGGACTCGCTCGTGACTCGGATAAGAATCGGTCGGTGATTTCCTCGCTCAACGCTCGTGAAATTCTTCTTGATATTGTGTTTTCGAATTTGGATTTGGATTTGGATTTGGATTTGGATTCGGATTCGGTTTCCCCTGATTTGAGCCGCGAATCGCTCGCGTTGCTTGTGATGTTGCCGCTCACGGAATCTGAGTGCGTTCTCGTTGCGTCTGATCCGCAACGGATTGGTTACCTGTCACATCTTCTGTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCTGCGTTGATTGAAATTGTGATTGCTGGAACTCGAGCTTCGGAGCTTCGAGGTCAAATTTGCGCTATCGATGAATTGTTCGAAGGCGTGGTTGAGATTCTCAGAGATCCAACGGCGTATCCGCGAGCGCTCAAGGTCGGAGTTAAGGCCTTGTTCGCTCTCTGTCTGGTGAAACAGACCAGGCACAAGGCGGTTTCCGCAGGCGCCGCCGAGATTCTCGTCGACAGATTCCCCGATTTGGAGAAATACGACGCGGAACGAGCCCTAGCGACGATCGAACTCATATGCAGAATTCCGGCCGGTTGCGACGCGTTTGCCGCTCACGCGCTCACCGTTCCTCTGTTAGTGAAAGTCATTCTGAAGATCTCGGATAGAGCGACGGAATCGGCGGTGGGAGCTCTGGTGTCGCTGTGCTCGGCGTCGGAGGAGAACCGGCGGGAGGCGGTGGCGGCGGGGATTTTTACACAGTTGTTGTTGCTGGTGCAAAGCGATTGTACGGACCGCGTTAAGCGGAAAACGCAGGTGCTGCTGAAGCTTCTTCGGGATTCATGGCCGCAAGATTCCGTCGGAAATTCCGACGACTATGCTTGCAGTGAAATTGTGGTGCCGTTTTGTTAATTGTGGAGTCTCACATTTTGCATCTCTGATCTCCAGTTTGTTCTGTAAGTTGAAGATTCATCCGGGAAGAAAGAAGAAGGAAGAATTGTGAACTAGAGACGAACAGACAAAGTTGCAGTAGTTTTCTGTATGTAATTTTTGTACGTAAATTTTTTTGTTCGATAGCTTTCAGAAAAAAGAAAAATCCAAAAAAAAAAGAAAAAAAGAAAGGAATTATTTTCGTCTATTGTTAAGTTTTTATTTGAAAGATTTTTGACATTTGTAACAGGATTTCCTCTTAGTGATATTTTAACATCATTAATTTTCTCTTTTA

Coding sequence (CDS)

ATGCCTGGAAGTGTTGTTGTTGAACCCTTGGATTTGAGCGTACACATTCCATACCATTTCAGGTGTCCGATTTCTCTCGAGCTCATGCGCGACCCGGTTACGGTCAGCACTGGTCAGACCTACGACCGCTCCAGTATTGAGTCATGGGTGGCCACCGGCAACACCACCTGTCCGGTCACTAGGGCTCCGCTCACCGACTTCACGCTCATCCCCAACCATACGCTGCGACGCCTCATTCAGGATTGGTGCGTTGCAAACCGCTCTTATGGCGTCGAGCGGATTCCGACTCCGAAGCAACCGGCCGATCCGTCTTTGGTTCGGTCTCTGCTTGCTCAAGCTTCGTCGAAATCGAGTGGTTCGAGTTCCAGAATCTCGGCTCTGCGGCGGTTGAAAGGACTCGCTCGTGACTCGGATAAGAATCGGTCGGTGATTTCCTCGCTCAACGCTCGTGAAATTCTTCTTGATATTGTGTTTTCGAATTTGGATTTGGATTTGGATTTGGATTTGGATTCGGATTCGGTTTCCCCTGATTTGAGCCGCGAATCGCTCGCGTTGCTTGTGATGTTGCCGCTCACGGAATCTGAGTGCGTTCTCGTTGCGTCTGATCCGCAACGGATTGGTTACCTGTCACATCTTCTGTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCTGCGTTGATTGAAATTGTGATTGCTGGAACTCGAGCTTCGGAGCTTCGAGGTCAAATTTGCGCTATCGATGAATTGTTCGAAGGCGTGGTTGAGATTCTCAGAGATCCAACGGCGTATCCGCGAGCGCTCAAGGTCGGAGTTAAGGCCTTGTTCGCTCTCTGTCTGGTGAAACAGACCAGGCACAAGGCGGTTTCCGCAGGCGCCGCCGAGATTCTCGTCGACAGATTCCCCGATTTGGAGAAATACGACGCGGAACGAGCCCTAGCGACGATCGAACTCATATGCAGAATTCCGGCCGGTTGCGACGCGTTTGCCGCTCACGCGCTCACCGTTCCTCTGTTAGTGAAAGTCATTCTGAAGATCTCGGATAGAGCGACGGAATCGGCGGTGGGAGCTCTGGTGTCGCTGTGCTCGGCGTCGGAGGAGAACCGGCGGGAGGCGGTGGCGGCGGGGATTTTTACACAGTTGTTGTTGCTGGTGCAAAGCGATTGTACGGACCGCGTTAAGCGGAAAACGCAGGTGCTGCTGAAGCTTCTTCGGGATTCATGGCCGCAAGATTCCGTCGGAAATTCCGACGACTATGCTTGCAGTGAAATTGTGGTGCCGTTTTGTTAA

Protein sequence

MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYACSEIVVPFC
Homology
BLAST of Lcy11g005030 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.2e-150
Identity = 277/432 (64.12%), Postives = 347/432 (80.32%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPG+  +EPLDL + IPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCPVT
Sbjct: 1   MPGN--LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVT 60

Query: 61  RAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSS-- 120
           R  L+DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPADP  VRSLL+QAS+ +   
Sbjct: 61  RVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 120

Query: 121 -GSSSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPD 180
               SR +A+RRL+GLARDS+KNR +I+  NAREIL+ I+F+        D+++ S+S +
Sbjct: 121 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFA--------DIETTSLSSE 180

Query: 181 LSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRA 240
           L  ESLALLV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAAALIE+V+ G ++
Sbjct: 181 LVSESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKS 240

Query: 241 SELRGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIL 300
            +L+  I   D +FEGV+++L++P +  RALK+G+KA+FALCLVKQTRH A+SAGA  IL
Sbjct: 241 MDLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGIL 300

Query: 301 VDRF-PDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVG 360
           +DR   D ++ D ER LAT+EL+CR+P GC AF  HALTVPL+VK IL++SDRATE A G
Sbjct: 301 IDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAG 360

Query: 361 ALVSLCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNS 420
           AL++LC+A E  R EA AAG+ TQLLLLVQSDCT+R KRK Q+LLKLLRDSWP DS  +S
Sbjct: 361 ALLALCTAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 420

Query: 421 DDYACSEIVVPF 429
           DD+  SE V PF
Sbjct: 421 DDFNRSE-VAPF 421

BLAST of Lcy11g005030 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 5.7e-146
Identity = 274/429 (63.87%), Postives = 338/429 (78.79%), Query Frame = 0

Query: 7   VEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 66
           +EPLDL + IPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 67  DFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSS---GSSS 126
           DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPADP+ VR+LL+QAS+ +       S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 127 RISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRES 186
           R +ALRRL+G ARDSDKNR +I++ NA EIL+ I+F            S++ S +L  ES
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF------------SETTSSELVSES 184

Query: 187 LALLVMLPLTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELR 246
           LALLVMLP+TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIEIV  GT++++L+
Sbjct: 185 LALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLK 244

Query: 247 GQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRF 306
           G I   + +FEGV+++LR+P +  RALK+G+K LFALC VK TRH A++AGA EIL+DR 
Sbjct: 245 GSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRL 304

Query: 307 -PDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 366
             D ++ D ERALAT+EL+CR P GC AF  HALTVPLLVK IL++SDRATE A GAL++
Sbjct: 305 AADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLA 364

Query: 367 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQ-DSVGNSDDY 426
           LC+A E  R EA  AG+  QLLL+VQS+CT+R K+K Q LLKLLRDSWP  +S  NSDD+
Sbjct: 365 LCTAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDF 421

Query: 427 ACSEIVVPF 429
            CS  VVPF
Sbjct: 425 GCSSQVVPF 421

BLAST of Lcy11g005030 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.2e-49
Identity = 134/409 (32.76%), Postives = 212/409 (51.83%), Query Frame = 0

Query: 10  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 69
           +D  + IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +TD  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 70  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSSRISALR 129
           L PNHTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+  +   +S   +++  L+
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDS---ASSHENQVKCLK 125

Query: 130 RLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRESLALLVM 189
           RL+ +  ++  N+  + +    E L +IV             +DS +  L+ E+L LL  
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIV------------SNDSENGSLTDEALNLLYH 185

Query: 190 LPLTES---ECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQIC 249
           L  +E+     +    D   +  L+ ++     E R+ A  L++ ++     ++    + 
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL---EVADPMQSMT 245

Query: 250 AIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFPD-- 309
              E+F  VV+IL D  +  +A K  +  L  +C   + RHKAV AG   ++++   D  
Sbjct: 246 LKPEVFTEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDES 305

Query: 310 --LEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSL 369
              E+   E A+  ++L+C+   G   F  H   + ++ K IL++S  A++ AV  L+S+
Sbjct: 306 FTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSV 365

Query: 370 --CSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSW 409
               A+     E +  G+  +L L++Q  C  + K K + LLKL    W
Sbjct: 366 GRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Lcy11g005030 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 7.9e-47
Identity = 137/426 (32.16%), Postives = 222/426 (52.11%), Query Frame = 0

Query: 10  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 69
           +D  + IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +T+  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 70  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSSRISALR 129
           L PNHTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ ++   SS   +++  L+
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKES---SSSHLNQVKCLK 120

Query: 130 RLKGLARDSDKNRSVISSLNAREILLDIVFSNLDL--DLDLDLDSDSVSP---------- 189
           RL+ +  ++  N+  + +    E L +IV +++D        L S +++           
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 190 -DLSRESL--ALLVMLPLTESECVLVA-----SDPQRIGYLSHLLFDSSIEVRINAAALI 249
            D SR  +  AL V+  L  SE  L +          +  L+ ++     E R  AA L+
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 250 EIVIAGTRASELRGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 309
           + ++      ++   I    ELF  V++IL D  ++ +A +  ++ L   C   + RHKA
Sbjct: 241 KKLLEVADPMQI---ILLERELFGEVIQILHDQISH-KATRSAMQILVITCPWGRNRHKA 300

Query: 310 VSAGA----AEILVDRFPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVIL 369
           V  G      E+L+D     E+ ++E A+  ++++C+   G   F  H   + ++ K IL
Sbjct: 301 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 360

Query: 370 KISDRATESAVGALVSL--CSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLK 409
           ++S   +E AV  L+S+    A+    +E +  G+  +L L++Q  C ++ K K + LLK
Sbjct: 361 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 419

BLAST of Lcy11g005030 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.6e-42
Identity = 128/396 (32.32%), Postives = 207/396 (52.27%), Query Frame = 0

Query: 14  VHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNH 73
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPNH
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNH 88

Query: 74  TLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKS-SGSSSRISA-LRRLK 133
           T+R++IQ WCV   S  ++RIPTP+ P  P  V  +  + SS +  G   +    + ++K
Sbjct: 89  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKIK 148

Query: 134 GLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRESLALLVMLPL 193
            L  +S+KNR  ++  +   +L D  F     D  L          +  E L+LL  +  
Sbjct: 149 KLGDESEKNRKCVNENSVGWVLCD-CFDKFSGDEKLTF--------MLNEILSLLTWMFP 208

Query: 194 TESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDE-L 253
              E +   +       ++ LL  +   VR NAA +++ +++     E R    A++  +
Sbjct: 209 IGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS---LDETRVHSFAVENGV 268

Query: 254 FEGVVEILRDPTAYPRALKVGVKALFALCLVK-QTRHKAVSAGAAEILVDRFPDLEKYDA 313
            E +V+++RD  +   + K  + A++ + L K +   + +  G   I V+   D E    
Sbjct: 269 AEALVKLIRDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVC 328

Query: 314 ERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEENR 373
           E+ALA ++ IC    G +    +AL +PLLVK I K+S+ AT S++  ++ L        
Sbjct: 329 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVA 388

Query: 374 -REAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLL 405
             +AV  G F ++LL++Q    +  K K   LLK++
Sbjct: 389 VEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMM 411

BLAST of Lcy11g005030 vs. ExPASy TrEMBL
Match: A0A6J1IYF7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480329 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.2e-219
Identity = 403/428 (94.16%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGS 120
           RAPLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPADPSLVRSLL QASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSR 180
           SSRISA+RRLKGLARDS+KNRS+ISSLNAREIL+D+VFSN         DSDSVSPDLSR
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--------SDSDSVSPDLSR 180

Query: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240
           ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL
Sbjct: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240

Query: 241 RGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDR 300
           RGQICAIDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEIL+DR
Sbjct: 241 RGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDR 300

Query: 301 FPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360
           FPDLEKYDAERALATIELICRIP G DAFAAHALTVPLLVKVILKISDRATESAVGALVS
Sbjct: 301 FPDLEKYDAERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360

Query: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYA 420
           LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRK+QVLLKLLRDSWPQDSVGNSDDY 
Sbjct: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYG 420

Query: 421 CSEIVVPF 429
           CSEIV+PF
Sbjct: 421 CSEIVIPF 420

BLAST of Lcy11g005030 vs. ExPASy TrEMBL
Match: A0A6J1FSF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447739 PE=4 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.4e-218
Identity = 401/428 (93.69%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGS 120
           RAPLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPADPSLVRSLL QASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSR 180
           SSRISA+RRLKGLARDS+KNRS+ISSLNAREIL+D+VFSN         DSDSVSPDLSR
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--------SDSDSVSPDLSR 180

Query: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240
           ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL
Sbjct: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240

Query: 241 RGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDR 300
           RGQICAIDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEIL+DR
Sbjct: 241 RGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDR 300

Query: 301 FPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360
           FPDLEKYDAERALATIELICRIP G DAFA+HALTVPLLVKVILKISDRATESAVGALVS
Sbjct: 301 FPDLEKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVS 360

Query: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYA 420
           LCSASEENRREAVAAGIFTQLLLLVQSDCT+RVKRK+QVLLKLLRDSWPQDSVGNSDDY 
Sbjct: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYG 420

Query: 421 CSEIVVPF 429
           CSEIV+PF
Sbjct: 421 CSEIVIPF 420

BLAST of Lcy11g005030 vs. ExPASy TrEMBL
Match: A0A5A7T4S1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001500 PE=4 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.4e-216
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0

Query: 3   GSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSS 122
           PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPA+PSLVRSLL QASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124

Query: 123 RISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRES 182
           RISALRRLKGLARDSDKNRS+ISSLNAREILLD+VFSN      LD  SDS SPDLSRES
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSN------LDSGSDSFSPDLSRES 184

Query: 183 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRG 242
           LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR 
Sbjct: 185 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRT 244

Query: 243 QICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFP 302
           QIC IDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEI++DRFP
Sbjct: 245 QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFP 304

Query: 303 DLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 362
           DLEKYDAERALATIELICR+P GCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC
Sbjct: 305 DLEKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 364

Query: 363 SASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYACS 422
           SASEENRREAVAAGI TQLLLLVQSDCT+RVKRK+QVLLKLLRDSWPQ+SVGNSDDY CS
Sbjct: 365 SASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCS 424

Query: 423 EIVVPFC 430
           EIVVPFC
Sbjct: 425 EIVVPFC 425

BLAST of Lcy11g005030 vs. ExPASy TrEMBL
Match: A0A1S3C380 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496167 PE=4 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.4e-216
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0

Query: 3   GSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSS 122
           PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPA+PSLVRSLL QASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124

Query: 123 RISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRES 182
           RISALRRLKGLARDSDKNRS+ISSLNAREILLD+VFSN      LD  SDS SPDLSRES
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSN------LDSGSDSFSPDLSRES 184

Query: 183 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRG 242
           LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR 
Sbjct: 185 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRT 244

Query: 243 QICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFP 302
           QIC IDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEI++DRFP
Sbjct: 245 QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFP 304

Query: 303 DLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 362
           DLEKYDAERALATIELICR+P GCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC
Sbjct: 305 DLEKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 364

Query: 363 SASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYACS 422
           SASEENRREAVAAGI TQLLLLVQSDCT+RVKRK+QVLLKLLRDSWPQ+SVGNSDDY CS
Sbjct: 365 SASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCS 424

Query: 423 EIVVPFC 430
           EIVVPFC
Sbjct: 425 EIVVPFC 425

BLAST of Lcy11g005030 vs. ExPASy TrEMBL
Match: A0A0A0L6F8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G203270 PE=4 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.4e-216
Identity = 398/427 (93.21%), Postives = 410/427 (96.02%), Query Frame = 0

Query: 3   GSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSS 122
           PLTDFTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLLAQASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPAEPSLVRSLLAQASSRSSGSSL 124

Query: 123 RISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRES 182
           RISALRRLKGLARDSDKNRS+ISSLNAREILLD+VFSN      LD  SDS SPDLSRES
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSN------LDSGSDSFSPDLSRES 184

Query: 183 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRG 242
           LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIE RINAAALIE VIAGTRASELR 
Sbjct: 185 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEDRINAAALIETVIAGTRASELRT 244

Query: 243 QICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFP 302
           QIC IDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEI++DRFP
Sbjct: 245 QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFP 304

Query: 303 DLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 362
           DLEKYDAERALATIELICRIP GCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC
Sbjct: 305 DLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 364

Query: 363 SASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYACS 422
           SASEENRREAVAAGI TQLLLLVQSDCT+RVKRK+QVLLKLLRDSWPQ+SVGNSDDYACS
Sbjct: 365 SASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYACS 424

Query: 423 EIVVPFC 430
           EIVVPFC
Sbjct: 425 EIVVPFC 425

BLAST of Lcy11g005030 vs. NCBI nr
Match: XP_022981075.1 (U-box domain-containing protein 26-like [Cucurbita maxima])

HSP 1 Score: 770.0 bits (1987), Expect = 1.1e-218
Identity = 403/428 (94.16%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGS 120
           RAPLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPADPSLVRSLL QASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSR 180
           SSRISA+RRLKGLARDS+KNRS+ISSLNAREIL+D+VFSN         DSDSVSPDLSR
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--------SDSDSVSPDLSR 180

Query: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240
           ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL
Sbjct: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240

Query: 241 RGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDR 300
           RGQICAIDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEIL+DR
Sbjct: 241 RGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDR 300

Query: 301 FPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360
           FPDLEKYDAERALATIELICRIP G DAFAAHALTVPLLVKVILKISDRATESAVGALVS
Sbjct: 301 FPDLEKYDAERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360

Query: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYA 420
           LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRK+QVLLKLLRDSWPQDSVGNSDDY 
Sbjct: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYG 420

Query: 421 CSEIVVPF 429
           CSEIV+PF
Sbjct: 421 CSEIVIPF 420

BLAST of Lcy11g005030 vs. NCBI nr
Match: XP_023547970.1 (U-box domain-containing protein 26-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 5.3e-218
Identity = 402/428 (93.93%), Postives = 413/428 (96.50%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGS 120
           RAPLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPADPSLVRSLL QASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSR 180
           SSRISA+RRLKGLARDS+KNR +ISSLNAREIL+D+VFSN         DSDSVSPDLSR
Sbjct: 121 SSRISAVRRLKGLARDSEKNRLLISSLNAREILIDVVFSN--------SDSDSVSPDLSR 180

Query: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240
           ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL
Sbjct: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240

Query: 241 RGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDR 300
           RGQICAIDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEIL+DR
Sbjct: 241 RGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDR 300

Query: 301 FPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360
           FPDLEKYDAERALATIELICRIP G DAFAAHALTVPLLVKVILKISDRATESAVGALVS
Sbjct: 301 FPDLEKYDAERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360

Query: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYA 420
           LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRK+QVLLKLLRDSWPQDSVGNSDDY 
Sbjct: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYG 420

Query: 421 CSEIVVPF 429
           CSEIV+PF
Sbjct: 421 CSEIVIPF 420

BLAST of Lcy11g005030 vs. NCBI nr
Match: XP_022942824.1 (U-box domain-containing protein 26-like [Cucurbita moschata] >KAG6600321.1 U-box domain-containing protein 26, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030978.1 U-box domain-containing protein 26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767.3 bits (1980), Expect = 7.0e-218
Identity = 401/428 (93.69%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGS 120
           RAPLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPADPSLVRSLL QASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSR 180
           SSRISA+RRLKGLARDS+KNRS+ISSLNAREIL+D+VFSN         DSDSVSPDLSR
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--------SDSDSVSPDLSR 180

Query: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240
           ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL
Sbjct: 181 ESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASEL 240

Query: 241 RGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDR 300
           RGQICAIDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEIL+DR
Sbjct: 241 RGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDR 300

Query: 301 FPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360
           FPDLEKYDAERALATIELICRIP G DAFA+HALTVPLLVKVILKISDRATESAVGALVS
Sbjct: 301 FPDLEKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVS 360

Query: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYA 420
           LCSASEENRREAVAAGIFTQLLLLVQSDCT+RVKRK+QVLLKLLRDSWPQDSVGNSDDY 
Sbjct: 361 LCSASEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYG 420

Query: 421 CSEIVVPF 429
           CSEIV+PF
Sbjct: 421 CSEIVIPF 420

BLAST of Lcy11g005030 vs. NCBI nr
Match: XP_038875257.1 (U-box domain-containing protein 26-like isoform X2 [Benincasa hispida] >XP_038875267.1 U-box domain-containing protein 26-like isoform X3 [Benincasa hispida])

HSP 1 Score: 765.0 bits (1974), Expect = 3.5e-217
Identity = 399/427 (93.44%), Postives = 410/427 (96.02%), Query Frame = 0

Query: 3   GSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV VEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSS 122
           PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPA+PSLVRSLL+QASS+SSG + 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQASSRSSGLNL 124

Query: 123 RISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRES 182
           RISALRRLKGLARDSDKNRS+ISSLNAREILLD+VFSN      LD DSDS SPDLSRES
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSN------LDSDSDSFSPDLSRES 184

Query: 183 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRG 242
           LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR 
Sbjct: 185 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRS 244

Query: 243 QICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFP 302
           QIC IDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEI++DRFP
Sbjct: 245 QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFP 304

Query: 303 DLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 362
           DLEKYDAERALATIELICRIP GCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC
Sbjct: 305 DLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 364

Query: 363 SASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYACS 422
           SASEENRREAVAAGI TQLLLLVQSDCT+RVKRK+QVLLKLLRDSWPQ+SVGNSDDY CS
Sbjct: 365 SASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCS 424

Query: 423 EIVVPFC 430
           EIVVPFC
Sbjct: 425 EIVVPFC 425

BLAST of Lcy11g005030 vs. NCBI nr
Match: XP_008456137.1 (PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0037126.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa] >TYK13948.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa])

HSP 1 Score: 761.9 bits (1966), Expect = 2.9e-216
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0

Query: 3   GSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSS 122
           PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPA+PSLVRSLL QASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124

Query: 123 RISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRES 182
           RISALRRLKGLARDSDKNRS+ISSLNAREILLD+VFSN      LD  SDS SPDLSRES
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSN------LDSGSDSFSPDLSRES 184

Query: 183 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRG 242
           LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR 
Sbjct: 185 LALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRT 244

Query: 243 QICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFP 302
           QIC IDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEI++DRFP
Sbjct: 245 QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFP 304

Query: 303 DLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 362
           DLEKYDAERALATIELICR+P GCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC
Sbjct: 305 DLEKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLC 364

Query: 363 SASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNSDDYACS 422
           SASEENRREAVAAGI TQLLLLVQSDCT+RVKRK+QVLLKLLRDSWPQ+SVGNSDDY CS
Sbjct: 365 SASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCS 424

Query: 423 EIVVPFC 430
           EIVVPFC
Sbjct: 425 EIVVPFC 425

BLAST of Lcy11g005030 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 533.5 bits (1373), Expect = 1.6e-151
Identity = 277/432 (64.12%), Postives = 347/432 (80.32%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPG+  +EPLDL + IPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCPVT
Sbjct: 1   MPGN--LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVT 60

Query: 61  RAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSS-- 120
           R  L+DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPADP  VRSLL+QAS+ +   
Sbjct: 61  RVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 120

Query: 121 -GSSSRISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPD 180
               SR +A+RRL+GLARDS+KNR +I+  NAREIL+ I+F+        D+++ S+S +
Sbjct: 121 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFA--------DIETTSLSSE 180

Query: 181 LSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRA 240
           L  ESLALLV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAAALIE+V+ G ++
Sbjct: 181 LVSESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKS 240

Query: 241 SELRGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIL 300
            +L+  I   D +FEGV+++L++P +  RALK+G+KA+FALCLVKQTRH A+SAGA  IL
Sbjct: 241 MDLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGIL 300

Query: 301 VDRF-PDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVG 360
           +DR   D ++ D ER LAT+EL+CR+P GC AF  HALTVPL+VK IL++SDRATE A G
Sbjct: 301 IDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAG 360

Query: 361 ALVSLCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQDSVGNS 420
           AL++LC+A E  R EA AAG+ TQLLLLVQSDCT+R KRK Q+LLKLLRDSWP DS  +S
Sbjct: 361 ALLALCTAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 420

Query: 421 DDYACSEIVVPF 429
           DD+  SE V PF
Sbjct: 421 DDFNRSE-VAPF 421

BLAST of Lcy11g005030 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 518.8 bits (1335), Expect = 4.0e-147
Identity = 274/429 (63.87%), Postives = 338/429 (78.79%), Query Frame = 0

Query: 7   VEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 66
           +EPLDL + IPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 67  DFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSS---GSSS 126
           DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPADP+ VR+LL+QAS+ +       S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 127 RISALRRLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRES 186
           R +ALRRL+G ARDSDKNR +I++ NA EIL+ I+F            S++ S +L  ES
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF------------SETTSSELVSES 184

Query: 187 LALLVMLPLTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELR 246
           LALLVMLP+TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIEIV  GT++++L+
Sbjct: 185 LALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLK 244

Query: 247 GQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRF 306
           G I   + +FEGV+++LR+P +  RALK+G+K LFALC VK TRH A++AGA EIL+DR 
Sbjct: 245 GSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRL 304

Query: 307 -PDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 366
             D ++ D ERALAT+EL+CR P GC AF  HALTVPLLVK IL++SDRATE A GAL++
Sbjct: 305 AADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLA 364

Query: 367 LCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSWPQ-DSVGNSDDY 426
           LC+A E  R EA  AG+  QLLL+VQS+CT+R K+K Q LLKLLRDSWP  +S  NSDD+
Sbjct: 365 LCTAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDF 421

Query: 427 ACSEIVVPF 429
            CS  VVPF
Sbjct: 425 GCSSQVVPF 421

BLAST of Lcy11g005030 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-50
Identity = 134/409 (32.76%), Postives = 212/409 (51.83%), Query Frame = 0

Query: 10  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 69
           +D  + IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +TD  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 70  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSSRISALR 129
           L PNHTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+  +   +S   +++  L+
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDS---ASSHENQVKCLK 125

Query: 130 RLKGLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRESLALLVM 189
           RL+ +  ++  N+  + +    E L +IV             +DS +  L+ E+L LL  
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIV------------SNDSENGSLTDEALNLLYH 185

Query: 190 LPLTES---ECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQIC 249
           L  +E+     +    D   +  L+ ++     E R+ A  L++ ++     ++    + 
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL---EVADPMQSMT 245

Query: 250 AIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEILVDRFPD-- 309
              E+F  VV+IL D  +  +A K  +  L  +C   + RHKAV AG   ++++   D  
Sbjct: 246 LKPEVFTEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDES 305

Query: 310 --LEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSL 369
              E+   E A+  ++L+C+   G   F  H   + ++ K IL++S  A++ AV  L+S+
Sbjct: 306 FTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSV 365

Query: 370 --CSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLLRDSW 409
               A+     E +  G+  +L L++Q  C  + K K + LLKL    W
Sbjct: 366 GRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Lcy11g005030 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 189.5 bits (480), Expect = 5.6e-48
Identity = 137/426 (32.16%), Postives = 222/426 (52.11%), Query Frame = 0

Query: 10  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 69
           +D  + IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +T+  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 70  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKSSGSSSRISALR 129
           L PNHTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ ++   SS   +++  L+
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKES---SSSHLNQVKCLK 120

Query: 130 RLKGLARDSDKNRSVISSLNAREILLDIVFSNLDL--DLDLDLDSDSVSP---------- 189
           RL+ +  ++  N+  + +    E L +IV +++D        L S +++           
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 190 -DLSRESL--ALLVMLPLTESECVLVA-----SDPQRIGYLSHLLFDSSIEVRINAAALI 249
            D SR  +  AL V+  L  SE  L +          +  L+ ++     E R  AA L+
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 250 EIVIAGTRASELRGQICAIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 309
           + ++      ++   I    ELF  V++IL D  ++ +A +  ++ L   C   + RHKA
Sbjct: 241 KKLLEVADPMQI---ILLERELFGEVIQILHDQISH-KATRSAMQILVITCPWGRNRHKA 300

Query: 310 VSAGA----AEILVDRFPDLEKYDAERALATIELICRIPAGCDAFAAHALTVPLLVKVIL 369
           V  G      E+L+D     E+ ++E A+  ++++C+   G   F  H   + ++ K IL
Sbjct: 301 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 360

Query: 370 KISDRATESAVGALVSL--CSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLK 409
           ++S   +E AV  L+S+    A+    +E +  G+  +L L++Q  C ++ K K + LLK
Sbjct: 361 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 419

BLAST of Lcy11g005030 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.9e-43
Identity = 128/396 (32.32%), Postives = 207/396 (52.27%), Query Frame = 0

Query: 14  VHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNH 73
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPNH
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNH 88

Query: 74  TLRRLIQDWCVANRSYGVERIPTPKQPADPSLVRSLLAQASSKS-SGSSSRISA-LRRLK 133
           T+R++IQ WCV   S  ++RIPTP+ P  P  V  +  + SS +  G   +    + ++K
Sbjct: 89  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKIK 148

Query: 134 GLARDSDKNRSVISSLNAREILLDIVFSNLDLDLDLDLDSDSVSPDLSRESLALLVMLPL 193
            L  +S+KNR  ++  +   +L D  F     D  L          +  E L+LL  +  
Sbjct: 149 KLGDESEKNRKCVNENSVGWVLCD-CFDKFSGDEKLTF--------MLNEILSLLTWMFP 208

Query: 194 TESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDE-L 253
              E +   +       ++ LL  +   VR NAA +++ +++     E R    A++  +
Sbjct: 209 IGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS---LDETRVHSFAVENGV 268

Query: 254 FEGVVEILRDPTAYPRALKVGVKALFALCLVK-QTRHKAVSAGAAEILVDRFPDLEKYDA 313
            E +V+++RD  +   + K  + A++ + L K +   + +  G   I V+   D E    
Sbjct: 269 AEALVKLIRDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVC 328

Query: 314 ERALATIELICRIPAGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEENR 373
           E+ALA ++ IC    G +    +AL +PLLVK I K+S+ AT S++  ++ L        
Sbjct: 329 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVA 388

Query: 374 -REAVAAGIFTQLLLLVQSDCTDRVKRKTQVLLKLL 405
             +AV  G F ++LL++Q    +  K K   LLK++
Sbjct: 389 VEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMM 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FXA42.2e-15064.12U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9LT795.7e-14663.87U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q84TG32.2e-4932.76E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC67.9e-4732.16E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q5PNY62.6e-4232.32U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1IYF75.2e-21994.16RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480329 P... [more]
A0A6J1FSF93.4e-21893.69RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447739... [more]
A0A5A7T4S11.4e-21692.97RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C3801.4e-21692.97RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496167 PE=4 ... [more]
A0A0A0L6F82.4e-21693.21RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G203270 PE... [more]
Match NameE-valueIdentityDescription
XP_022981075.11.1e-21894.16U-box domain-containing protein 26-like [Cucurbita maxima][more]
XP_023547970.15.3e-21893.93U-box domain-containing protein 26-like [Cucurbita pepo subsp. pepo][more]
XP_022942824.17.0e-21893.69U-box domain-containing protein 26-like [Cucurbita moschata] >KAG6600321.1 U-box... [more]
XP_038875257.13.5e-21793.44U-box domain-containing protein 26-like isoform X2 [Benincasa hispida] >XP_03887... [more]
XP_008456137.12.9e-21692.97PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0037126.1 ... [more]
Match NameE-valueIdentityDescription
AT1G49780.11.6e-15164.12plant U-box 26 [more]
AT3G19380.14.0e-14763.87plant U-box 25 [more]
AT2G35930.11.6e-5032.76plant U-box 23 [more]
AT3G52450.15.6e-4832.16plant U-box 22 [more]
AT5G37490.11.9e-4332.32ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 19..82
e-value: 3.8E-34
score: 129.4
IPR003613U box domainPFAMPF04564U-boxcoord: 16..87
e-value: 2.2E-21
score: 75.8
IPR003613U box domainPROSITEPS51698U_BOXcoord: 15..89
score: 43.876438
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 93..412
e-value: 5.7E-15
score: 56.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 15..89
e-value: 8.2E-30
score: 104.7
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 7..425
NoneNo IPR availablePANTHERPTHR22849:SF141RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 7..425
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 20..61
e-value: 3.22556E-25
score: 94.993
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 13..91
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 106..404

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy11g005030.1Lcy11g005030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity