Lcy10g017060 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g017060
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMechanosensitive ion channel protein 1
LocationChr10: 45143518 .. 45146804 (+)
RNA-Seq ExpressionLcy10g017060
SyntenyLcy10g017060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTTTTCATTCTGAAGTCGATTTGTAGTTCATCAAGCACTTGTTCCTCCTTTCATTCTTACAAGTCTTACACAAAGATTTCCGAGTACATTGACCGGGTTCTTAGACCAAGTGCTGTTGTAAATAAAAAATACTATGAAGATGAATCTCACAGCTTGAAGAACACCCCACGTACTGGTTTACTAAGAACAACCTCTGTATGTCGTTTTCATATCCCCATTTATGGTACACATAGCATTGGTACAATTTTGCCCGCATCGTCAGTGAAATTTTGGAACACTATCCCTCTTACGTCACTCGACCATTTGTCAAACCCTCGATTATATTCATCTGCCTCTAGCAGTAAAGTGGATGTAACTAGAGATGTTCCAGCAGCTTCTTCTGGTGTTGAGGCGGATATCAGTAATCCCCCTGATCTTGGAAGGGACTGGCTTGAAAGAGTTAAGGATGTCTGGCAAAGTGTTGTAGATGCAACATCTTTTACTGGGCAAAAGGCTAGAGAAGTGTCTGATGAATTGTCTCCTCATGCAGATAAGCTATTTGATTCACATCCATATCTAAAAAATGTAATAGTTCCTCTAAGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCATAAGTATTCAATGCGAGGTCCTGTTGCGATACTCTCCATACCTGTTGAAGAAATTCCTTATGAGAAAAGTTTTTGGGGTGCCTTAGAGGATCCTTTGCTATATCTTGTCACATTTTTTGCAGTTTCTCAAATGTTAGTGACTCAAACTTCAACATAAATCTCTTGATCGCTCAATGGTTTTGATCTTCCCTAACAATATTTGTCTAAATTTTGATTTTGCAGTGGTACGATGGTTGCACCAACATCGATGACATCAGAGTTTGTTACACAGGCGTGCAGAGGGGCTGTCATTCTTTCCCTTGTCTGGTTTATTTATCGCTGGAAAAGAAATGTGTTATCTCGAGCTTTAGCCTCCCAGACTTTTGCAGGGCTTAATCGGAAATGGTTACTTACACTAGACAAGGTTTCATCTGTTGGCCTTTTTGCAATTGGATTAATGGGTTTGGCCGAGGCATCTGGGGTGGCTGTTCAATCTATGTTAACTGTTGGTGGCATTGGAGGTGAGAGCTTGCTGCCAATTGTCTTTGCAGTTTTAATTATTGATTCCTAGTTTGTGGTCATGTGTGAACTTGGATTTGTTTGTTGGTAGGGTTGAAGATTTAGCATTATTCATTTGCTTCTGTTTTGGTTTTTTTCTTCTTCTTGAAAAAAGAGAACTCTCCTCTTGATAATTAAACATTATTCATGTAGCCACTTTTGTATTGTTGGTAAGTTGAGATATGCTTGAGATAACAATCATGTTCCTTCTTGGCAATGTTATGCTTGCTTGTTGTTAATAATGTGGAACATGTGTAGCATACTTTAAATATCTTTTCCTTTTAAGGAGTGACCATTGCTTTTGCTGCGAGGGATATACGTGGGAATATTTTAAGTGGCTTAAGAATGCGGTTTTCACAACACTTCTCACTTGGAGATACGATTAAGGTGTGTGTAAATCATCTTTTTCCTGCATTTTTGGGTGAGGAGAGGAACATTATGAATTTATAATATGGTCGCATCCATTATGGTGTAAATCATCTTTTTCTTCTTTTAGTACAACATTCTTCAAATAAAAACATTACTGAAGTTGAGACCTCAATGGTGCTTAGACTTTGTATCTAGATGATCTTTTCATGCTACATTGTCATACTGTCATACATTATGGTGGAGTATTTTCTCTCTACATCTTTTTCCCTAGTTGTCTGTTCTCGAACCTGTAATCAAATGAATTGAAATTTTCTATTTGTAGGCTGGTGCTATTGAAGGTAAAGTTGTGGAAATGGGACTCATGACAACATCATTATTAAGTGCTGAACAATTTTCAATCGTTGTTCCAAATTCTCTCCTTTATGGCCAGGTGGATTTTTTTTTTTTTTTTAAAAAAAATCTGTCCTGAATATTTATTATTCTAGGCCTTTATTTTATTCCTATATTATCACTGTACCCTATTGGTCACTTGACATATTGAACTACATGCTTGCCTTGTTTAGATTTTTTAATAAAAGTCGAGCCATGTTTGATTAATGGTCTCCGTAGTTGAAGGATGCATTTGTAGACAATAATTTGATTAAGTACATTTGTTATCCTGGGAGATTTGAAGGGTTATTCGATTTCTATTTTCAATTAGGGTACAAGTTTGGGTAAAATTGTGATCCTGTAGAAGAGCATGTATGTTATGTTCAAATTCATTTTTCTGGAAGTCTATATCTGTGCATGTGTTGGCAATACTTTTCTTTGTTTTACTAATTTTGGAATTTTTCAACCAGACTTCTAAATTGAGTTAATTATTCATTTTAAATCTCTATAACTTTTTCTAAAAATATCGCATTTCTAACTCCCTTGAGGAGAAAATTCAAGCAATAATCATGACTCAAGTACTGGTGATGTTCTCGTCTTTTAACTCTGAAAACTTTCTTGCAATGCTATCTTCTATCTTCTATGTTTGAGTCTTGAATTTATGCGGAGATCCGCTTGCTATGCCATGTAGTAACTCAATACTTTCGCATGAATTTCTTAGCTTGATTATGCCCTCGTGGGATTTGCTCATTCAGTCTCATATGCTTCAACCCAATTTTCATGCAGTTATGTGTTGAGGATTGCGTTGAAGTTCTTGGATTAAGATAGTGGTTTAATCAATTTATTTACGGTGTAGTTACTCTAAAATGAGATGATTATATTTGCAGGAAATTATAAATGAGTCGCGGGCGCAGTGTCATGCAATCACCAAGAAAATTCCTTTGCAAGTAGATGATCTTAGTACCATTTCTCAGATATCAGATAATATTAAGAGTATGCTAAGATCTCACCCTCAAGTGTTCTTGGGCAAGGAGGCTCCTGATTGCTTCCCCTCTCAAGTTGAAAGTACTTGGGCAGAATTGACAATTAGTTGCAACCTTAAGAAGATGGTAAGTTTGACATTTTTAGTTACCTGTTAATGGTTTATGGTCGTGTTGTAACTTCTTGACGTGTCGTCTTTCAAACTCTACATTTACATTTCTTAAAATTGTATTTATTTAGGGTAAAGAAGAATTTTTCTCTACCCAGCAAGATATTCTTCTTCAGTCGATAAAGATCATCAAGGAAAATGGTCATGTCAGATTGGACGAACCAGTGATGATGAACAAGAGTGATTTATGCATGAAAGGGACTGAAGAGTAA

mRNA sequence

ATGGCGCTTTTCATTCTGAAGTCGATTTGTAGTTCATCAAGCACTTGTTCCTCCTTTCATTCTTACAAGTCTTACACAAAGATTTCCGAGTACATTGACCGGGTTCTTAGACCAAGTGCTGTTGTAAATAAAAAATACTATGAAGATGAATCTCACAGCTTGAAGAACACCCCACGTACTGGTTTACTAAGAACAACCTCTGTATGTCGTTTTCATATCCCCATTTATGGTACACATAGCATTGGTACAATTTTGCCCGCATCGTCAGTGAAATTTTGGAACACTATCCCTCTTACGTCACTCGACCATTTGTCAAACCCTCGATTATATTCATCTGCCTCTAGCAGTAAAGTGGATGTAACTAGAGATGTTCCAGCAGCTTCTTCTGGTGTTGAGGCGGATATCAGTAATCCCCCTGATCTTGGAAGGGACTGGCTTGAAAGAGTTAAGGATGTCTGGCAAAGTGTTGTAGATGCAACATCTTTTACTGGGCAAAAGGCTAGAGAAGTGTCTGATGAATTGTCTCCTCATGCAGATAAGCTATTTGATTCACATCCATATCTAAAAAATGTAATAGTTCCTCTAAGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCATAAGTATTCAATGCGAGGTCCTGTTGCGATACTCTCCATACCTGTTGAAGAAATTCCTTATGAGAAAAGTTTTTGGGGTGCCTTAGAGGATCCTTTGCTATATCTTGTCACATTTTTTGCAGTTTCTCAAATTGGTACGATGGTTGCACCAACATCGATGACATCAGAGTTTGTTACACAGGCGTGCAGAGGGGCTGTCATTCTTTCCCTTGTCTGGTTTATTTATCGCTGGAAAAGAAATGTGTTATCTCGAGCTTTAGCCTCCCAGACTTTTGCAGGGCTTAATCGGAAATGGTTACTTACACTAGACAAGGTTTCATCTGTTGGCCTTTTTGCAATTGGATTAATGGGTTTGGCCGAGGCATCTGGGGTGGCTGTTCAATCTATGTTAACTGTTGGTGGCATTGGAGGAGTGACCATTGCTTTTGCTGCGAGGGATATACGTGGGAATATTTTAAGTGGCTTAAGAATGCGGTTTTCACAACACTTCTCACTTGGAGATACGATTAAGGCTGGTGCTATTGAAGGTAAAGTTGTGGAAATGGGACTCATGACAACATCATTATTAAGTGCTGAACAATTTTCAATCGTTGTTCCAAATTCTCTCCTTTATGGCCAGGAAATTATAAATGAGTCGCGGGCGCAGTGTCATGCAATCACCAAGAAAATTCCTTTGCAAGTAGATGATCTTAGTACCATTTCTCAGATATCAGATAATATTAAGAGTATGCTAAGATCTCACCCTCAAGTGTTCTTGGGCAAGGAGGCTCCTGATTGCTTCCCCTCTCAAGTTGAAAGTACTTGGGCAGAATTGACAATTAGTTGCAACCTTAAGAAGATGGGTAAAGAAGAATTTTTCTCTACCCAGCAAGATATTCTTCTTCAGTCGATAAAGATCATCAAGGAAAATGGTCATGTCAGATTGGACGAACCAGTGATGATGAACAAGAGTGATTTATGCATGAAAGGGACTGAAGAGTAA

Coding sequence (CDS)

ATGGCGCTTTTCATTCTGAAGTCGATTTGTAGTTCATCAAGCACTTGTTCCTCCTTTCATTCTTACAAGTCTTACACAAAGATTTCCGAGTACATTGACCGGGTTCTTAGACCAAGTGCTGTTGTAAATAAAAAATACTATGAAGATGAATCTCACAGCTTGAAGAACACCCCACGTACTGGTTTACTAAGAACAACCTCTGTATGTCGTTTTCATATCCCCATTTATGGTACACATAGCATTGGTACAATTTTGCCCGCATCGTCAGTGAAATTTTGGAACACTATCCCTCTTACGTCACTCGACCATTTGTCAAACCCTCGATTATATTCATCTGCCTCTAGCAGTAAAGTGGATGTAACTAGAGATGTTCCAGCAGCTTCTTCTGGTGTTGAGGCGGATATCAGTAATCCCCCTGATCTTGGAAGGGACTGGCTTGAAAGAGTTAAGGATGTCTGGCAAAGTGTTGTAGATGCAACATCTTTTACTGGGCAAAAGGCTAGAGAAGTGTCTGATGAATTGTCTCCTCATGCAGATAAGCTATTTGATTCACATCCATATCTAAAAAATGTAATAGTTCCTCTAAGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCATAAGTATTCAATGCGAGGTCCTGTTGCGATACTCTCCATACCTGTTGAAGAAATTCCTTATGAGAAAAGTTTTTGGGGTGCCTTAGAGGATCCTTTGCTATATCTTGTCACATTTTTTGCAGTTTCTCAAATTGGTACGATGGTTGCACCAACATCGATGACATCAGAGTTTGTTACACAGGCGTGCAGAGGGGCTGTCATTCTTTCCCTTGTCTGGTTTATTTATCGCTGGAAAAGAAATGTGTTATCTCGAGCTTTAGCCTCCCAGACTTTTGCAGGGCTTAATCGGAAATGGTTACTTACACTAGACAAGGTTTCATCTGTTGGCCTTTTTGCAATTGGATTAATGGGTTTGGCCGAGGCATCTGGGGTGGCTGTTCAATCTATGTTAACTGTTGGTGGCATTGGAGGAGTGACCATTGCTTTTGCTGCGAGGGATATACGTGGGAATATTTTAAGTGGCTTAAGAATGCGGTTTTCACAACACTTCTCACTTGGAGATACGATTAAGGCTGGTGCTATTGAAGGTAAAGTTGTGGAAATGGGACTCATGACAACATCATTATTAAGTGCTGAACAATTTTCAATCGTTGTTCCAAATTCTCTCCTTTATGGCCAGGAAATTATAAATGAGTCGCGGGCGCAGTGTCATGCAATCACCAAGAAAATTCCTTTGCAAGTAGATGATCTTAGTACCATTTCTCAGATATCAGATAATATTAAGAGTATGCTAAGATCTCACCCTCAAGTGTTCTTGGGCAAGGAGGCTCCTGATTGCTTCCCCTCTCAAGTTGAAAGTACTTGGGCAGAATTGACAATTAGTTGCAACCTTAAGAAGATGGGTAAAGAAGAATTTTTCTCTACCCAGCAAGATATTCTTCTTCAGTCGATAAAGATCATCAAGGAAAATGGTCATGTCAGATTGGACGAACCAGTGATGATGAACAAGAGTGATTTATGCATGAAAGGGACTGAAGAGTAA

Protein sequence

MALFILKSICSSSSTCSSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNTPRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSKVDVTRDVPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELSPHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSIPVEEIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYRWKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIGGVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSIVVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEAPDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENGHVRLDEPVMMNKSDLCMKGTEE
Homology
BLAST of Lcy10g017060 vs. ExPASy Swiss-Prot
Match: Q8VZL4 (Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MSL1 PE=1 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.0e-136
Identity = 252/438 (57.53%), Postives = 333/438 (76.03%), Query Frame = 0

Query: 87  ASSVKFWNTI-PLTSLDHLSNPRLYSSASSSKVDVTRDVPAASSGVEADISNPPDLGRDW 146
           +S+++  N   P   L H S  R +SS S     +       +SGV     +    G DW
Sbjct: 55  SSNIRLHNDAKPFNYLGHSSYARAFSSKSDDFGSI------VASGVTGS-GDGNGNGNDW 114

Query: 147 LERVKDVWQSVVDATSFTGQKAREVSDELSPHADKLFDSHPYLKNVIVPLSMTLTATLLA 206
           +E+ KDV Q+ VDA + T +K ++VSDE+ PH  +  DS+PYLK+VIVP+S+T+T TL A
Sbjct: 115 VEKAKDVLQTSVDAVTETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFA 174

Query: 207 WVVMPRLLRRFHKYSMRGPVAILSIPV--EEIPYEKSFWGALEDPLLYLVTFFAVSQIGT 266
           WVVMPR+LRRFH Y+M+    +L +    E++PYEKSFWGALEDP  YLVTF A +QI  
Sbjct: 175 WVVMPRILRRFHTYAMQSSAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAA 234

Query: 267 MVAPTSMTSEFVTQACRGAVILSLVWFIYRWKRNVLSRALASQTFAGLNRKWLLTLDKVS 326
           MVAPT++ +++ +   +GAVILSLVWF+YRWK NV++R L++++F GL+R+ +LTLDKVS
Sbjct: 235 MVAPTTIAAQYFSPTVKGAVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVS 294

Query: 327 SVGLFAIGLMGLAEASGVAVQSMLTVGGIGGVTIAFAARDIRGNILSGLRMRFSQHFSLG 386
           SVGLFAIGLM  AEA GVAVQS+LTVGG+GGV  AFAARDI GN+LSGL M+FS+ FS+G
Sbjct: 295 SVGLFAIGLMASAEACGVAVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMG 354

Query: 387 DTIKAGAIEGKVVEMGLMTTSLLSAEQFSIVVPNSLLYGQEIINESRAQCHAITKKIPLQ 446
           DTIKAG++EG+V+EMGL TTSLL+AE+F ++VPNSL   Q I+N+SRAQ  AI  KIPLQ
Sbjct: 355 DTIKAGSVEGQVIEMGLTTTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQ 414

Query: 447 VDDLSTISQISDNIKSMLRSHPQVFLGKEAPDCFPSQVESTWAELTISCNLKKMGKEEFF 506
           +DDL  I QIS+ IK MLRS+ +VFLGKEAP C+ S+VE ++AELTI CNL +MGKEE +
Sbjct: 415 IDDLDMIPQISNEIKEMLRSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELY 474

Query: 507 STQQDILLQSIKIIKENG 522
           +TQQ++LL+++KIIK++G
Sbjct: 475 NTQQEVLLEAVKIIKKHG 485

BLAST of Lcy10g017060 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-18
Identity = 73/296 (24.66%), Postives = 139/296 (46.96%), Query Frame = 0

Query: 240 SFW---------GALEDPLLYLVTFFAVSQIGTMV--APTSMTSEFVTQACRGAVILSLV 299
           SFW         G L   + +L+    +S +G+++     +   +F+T      +  SL+
Sbjct: 27  SFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLI 86

Query: 300 WFIYRWKRNVLSRALASQTF-AGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSML 359
             I   K  +L      Q    G +      + ++  + +  + ++   E  G+++  +L
Sbjct: 87  AVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLL 146

Query: 360 TVGGIGGVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKA--GAIEGKVVEMGLMTTSL 419
           T GGIGG+ +  A +DI  N  SG+ + F + FS+GD I++    IEG V E+G   T +
Sbjct: 147 TFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKI 206

Query: 420 LSAEQFSIVVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHP 479
            + +   + VPNSL     + N  R     IT  I L+ +D + +  I + ++ ML++HP
Sbjct: 207 TTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHP 266

Query: 480 QVFLGKEAPDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
            +   ++    + +Q   +   + + C  K     E+ + QQD+ L+ I I++ +G
Sbjct: 267 AID-QRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHG 321

BLAST of Lcy10g017060 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-18
Identity = 73/296 (24.66%), Postives = 139/296 (46.96%), Query Frame = 0

Query: 240 SFW---------GALEDPLLYLVTFFAVSQIGTMV--APTSMTSEFVTQACRGAVILSLV 299
           SFW         G L   + +L+    +S +G+++     +   +F+T      +  SL+
Sbjct: 27  SFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLI 86

Query: 300 WFIYRWKRNVLSRALASQTF-AGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSML 359
             I   K  +L      Q    G +      + ++  + +  + ++   E  G+++  +L
Sbjct: 87  AVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLL 146

Query: 360 TVGGIGGVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKA--GAIEGKVVEMGLMTTSL 419
           T GGIGG+ +  A +DI  N  SG+ + F + FS+GD I++    IEG V E+G   T +
Sbjct: 147 TFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKI 206

Query: 420 LSAEQFSIVVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHP 479
            + +   + VPNSL     + N  R     IT  I L+ +D + +  I + ++ ML++HP
Sbjct: 207 TTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHP 266

Query: 480 QVFLGKEAPDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
            +   ++    + +Q   +   + + C  K     E+ + QQD+ L+ I I++ +G
Sbjct: 267 AID-QRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHG 321

BLAST of Lcy10g017060 vs. ExPASy Swiss-Prot
Match: O25170 (Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=HP_0415 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-14
Identity = 69/277 (24.91%), Postives = 130/277 (46.93%), Query Frame = 0

Query: 281 GAV-ILSLVWFI---YRWKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLA 340
           GAV I+ L W +   ++     L   +A+++     ++ +  + KV    +F + L+G+ 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNMATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 341 EASGVAVQSMLTVGGIGGVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVV 400
           +  G  V +++   GIGG+ +A A +D+  N  + + +     FS GD I  G +EG VV
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 401 EMGLMTTSLLSAEQFSIVVPNSLLYGQEIINESRAQCHAITKKIPLQV-----DDLSTIS 460
           EMGL  T++ + +   + VPNS L G+ I N SR +   + ++I +++        S + 
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSRRK---VGRRIKMEIGLTYSSSQSALQ 502

Query: 461 QISDNIKSMLRSHPQV---------------------------FLG-KEAPDCFPSQVES 520
               +IK ML +HP++                           FLG K     F  Q   
Sbjct: 503 LCVKDIKEMLENHPKIANGADSALQNVSDYRYMFKKDIVSIDDFLGYKNNLFVFLDQFAD 562

BLAST of Lcy10g017060 vs. ExPASy Swiss-Prot
Match: Q9ZKG7 (Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 / ATCC 700824) OX=85963 GN=jhp_0969 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.7e-14
Identity = 69/277 (24.91%), Postives = 130/277 (46.93%), Query Frame = 0

Query: 281 GAV-ILSLVWFI---YRWKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLA 340
           GAV I+ L W +   ++     L   +A+++     ++ +  + KV    +F + L+G+ 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNVATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 341 EASGVAVQSMLTVGGIGGVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVV 400
           +  G  V +++   GIGG+ +A A +D+  N  + + +     FS GD I  G +EG VV
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 401 EMGLMTTSLLSAEQFSIVVPNSLLYGQEIINESRAQCHAITKKIPLQV-----DDLSTIS 460
           EMGL  T++ + +   + VPNS L G+ I N SR +   + ++I +++        S + 
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSRRK---VGRRIKMEIGLTYSSSQSALQ 502

Query: 461 QISDNIKSMLRSHPQV---------------------------FLG-KEAPDCFPSQVES 520
               +IK ML +HP++                           FLG K     F  Q   
Sbjct: 503 LCVKDIKEMLENHPKIANGADSALQNASDYRYMFKKDIVSIDDFLGYKNNLFVFLDQFAD 562

BLAST of Lcy10g017060 vs. ExPASy TrEMBL
Match: A0A6J1I1Q4 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111468640 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 7.5e-223
Identity = 410/528 (77.65%), Postives = 450/528 (85.23%), Query Frame = 0

Query: 1   MALFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNT 60
           M LFIL SI S+S +     SF   KSYTKISEY++RVLRPS V+NK+YY+DESHSLKN 
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNV 60

Query: 61  PRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSK 120
            RTG +R  SVCR H P YGTH I T  PAS VK+WNTIP TSL +LSNPR YSS SS K
Sbjct: 61  SRTGFIRAPSVCRLHTPSYGTHPIATFSPASPVKYWNTIPPTSLAYLSNPRFYSSTSSRK 120

Query: 121 VDVTRD--VPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 180
            D+T D  VP ASSGVEADISNPPD+GRDWLERVKD WQS VDA SFTGQKAREVSDE S
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFS 180

Query: 181 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 240
           PH DK+ DSHPYLKN I P+ MTLTATLLAWVVMPRLLRRFHKYSMR PVAIL  S  +E
Sbjct: 181 PHVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPL YLVTF+A SQIG MVAPT++ SEFV+QACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 360
           WK NV SRALASQ F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 420
           GV  AFA+RDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+V+EMGLMTTSLLSAE+F I
Sbjct: 361 GVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 480
           VVPNSL   Q IIN+SRAQ  AITKKIP QV D ST+S+ISD+IKSMLRSHP+VFLGKEA
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEA 480

Query: 481 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+ES++AELTI CNLK+MGKEE FST+QDILLQS++IIKENG
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIIKENG 528

BLAST of Lcy10g017060 vs. ExPASy TrEMBL
Match: A0A6J1FHY5 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111444110 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 8.3e-222
Identity = 412/528 (78.03%), Postives = 449/528 (85.04%), Query Frame = 0

Query: 1   MALFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNT 60
           M LFIL SI S+S +     SF   KSYTKISEY++RVLR S V+NK+YY+DESHSLKN 
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNV 60

Query: 61  PRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSK 120
            RTG LR  SVC  H P YGTH   T  PAS VK+WNTIP TSL +LSNPR YSS SS K
Sbjct: 61  SRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120

Query: 121 VDVTRD--VPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 180
            D+T D  VP ASSGVEADISNPPD+GRDWLERVKD WQSVVDA SFTGQKAREVSDE S
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS 180

Query: 181 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 240
           PH DKL DSHPYLKN I P+ MTLTATLLAWVVMPRLLRRFHKYSMR PVAIL  S  +E
Sbjct: 181 PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPL YLVTF+A SQIG MVAPT++ SEFV+QACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 360
           WK NV SRALASQ F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 420
           GV  AFAARDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+VVEMGLMTTSLLSAE+F I
Sbjct: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 480
           VVPNSL   Q IIN+SRAQ  AITKKIP QV DLST+S+ISD+IKSMLRSHP+VFLGKEA
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPKVFLGKEA 480

Query: 481 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+ES++AELTI CNLK+MGKEE FST+QDILLQS++I+KENG
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENG 528

BLAST of Lcy10g017060 vs. ExPASy TrEMBL
Match: A0A6J1D280 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111016329 PE=3 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 3.4e-215
Identity = 404/528 (76.52%), Postives = 450/528 (85.23%), Query Frame = 0

Query: 3   LFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNTPR 62
           +FILKSI +SS +     S  SYK YTKISEY+DR LRPS V+NKK + D S   KNT  
Sbjct: 6   IFILKSIYTSSRSYLNPYSSQSYKPYTKISEYVDRGLRPSTVLNKKCHNDNSRRTKNTSG 65

Query: 63  TGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSKVD 122
           TG  R  SVC  +I  +G + I T LPA SV++W T+PLTS  HLSNPRLYSSASS K D
Sbjct: 66  TGFPRAASVCGLNISSHGAYPIDTFLPAWSVRYWRTVPLTSPGHLSNPRLYSSASSRKAD 125

Query: 123 VTRD--VPAASSGVEADISNPPDL--GRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 182
           +T D  V AAS+GVEADISN  D+  G++WLERVKDVWQS VDA SFT QKA+EVSDE +
Sbjct: 126 ITGDTGVSAASAGVEADISNTQDVGTGKEWLERVKDVWQSAVDAASFTEQKAKEVSDEFT 185

Query: 183 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 242
           PH DKLFDSHPYLKNVIVP+SMTLTATLLAW+VMPRLLRRFHKYSMRGPVA+L  SI VE
Sbjct: 186 PHVDKLFDSHPYLKNVIVPVSMTLTATLLAWLVMPRLLRRFHKYSMRGPVALLSGSISVE 245

Query: 243 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 302
           EIPYEKSFWGALEDPL YLVTFFA SQIG MVAPT++ SE+VTQACRGAVILSLVWFIYR
Sbjct: 246 EIPYEKSFWGALEDPLRYLVTFFAFSQIGMMVAPTTVASEYVTQACRGAVILSLVWFIYR 305

Query: 303 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 362
           WK NVLSRALASQ+F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 306 WKTNVLSRALASQSFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 365

Query: 363 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 422
           GV  AFAARDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+VVEMGLMTTSLLSAE+F I
Sbjct: 366 GVAAAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPI 425

Query: 423 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 482
           VVPNSL   Q I+N+SRAQ  AITKKIPLQVD+LST+SQISDNIKSMLRSHP+VFLGKEA
Sbjct: 426 VVPNSLFSSQVIVNKSRAQWRAITKKIPLQVDNLSTVSQISDNIKSMLRSHPKVFLGKEA 485

Query: 483 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+EST+AELTI CNL+KM K+E FST+QDILLQS++IIKE+G
Sbjct: 486 PYCFLSQIESTYAELTIGCNLRKMSKDELFSTEQDILLQSVQIIKEHG 533

BLAST of Lcy10g017060 vs. ExPASy TrEMBL
Match: A0A5A7U5P8 (Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001760 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.5e-210
Identity = 384/508 (75.59%), Postives = 429/508 (84.45%), Query Frame = 0

Query: 18  SFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNTPRTGLLRTTSVCRFHIPIYG 77
           SF S+K YTKIS+Y+DRVLRP+ V+NK+YY DESHS K           +VC  HIP YG
Sbjct: 41  SFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKR---------ATVCGLHIPSYG 100

Query: 78  THSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSKVDVTRD--VPAASSGVEADI 137
           TH I T LPASS+K+WNTIPLTS+ +LSN R YSS S  K D+T D  VPAAS+GVE DI
Sbjct: 101 THRIDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPDI 160

Query: 138 SNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELSPHADKLFDSHPYLKNVIVPL 197
           SNPPD+GRD LE+VKDVWQS VDA SFTGQKA+EVSDELSPH DKL DSHPYLKNVIVP+
Sbjct: 161 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 220

Query: 198 SMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILS--IPVEEIPYEKSFWGALEDPLLYLV 257
           SMTL  TLLAW VMPRLLRRFHKYSMR PVAI+S  +P EEIPYEKSFWGALEDPL YLV
Sbjct: 221 SMTLAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYLV 280

Query: 258 TFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYRWKRNVLSRALASQTFAGLNR 317
           TFFA SQIG +VAPT++  EFV+QACRGAVILSLVWFIYRWK NV SRALA+QT AGL+R
Sbjct: 281 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLDR 340

Query: 318 KWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIGGVTIAFAARDIRGNILSGLR 377
             LLTLDKVSSV LFAIGLM LAEASGVAVQSMLTVGGIGGV  AFAARD+ GNILSGL 
Sbjct: 341 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGLT 400

Query: 378 MRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSIVVPNSLLYGQEIINESRAQC 437
           M+FSQ FSLGDTIKAG IEG+VVEMGLMTTSLLSAE+F +VVPNSL   Q IIN+SRAQ 
Sbjct: 401 MQFSQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 460

Query: 438 HAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEAPDCFPSQVESTWAELTISCN 497
            AI K++PLQVDD+ST+SQI+D+IK ML SHP+VFLGKEAP CF SQ+EST+AELT+ CN
Sbjct: 461 RAIVKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGCN 520

Query: 498 LKKMGKEEFFSTQQDILLQSIKIIKENG 522
           LKKMGK+E FST+QDILLQS++IIK  G
Sbjct: 521 LKKMGKDESFSTEQDILLQSVQIIKARG 539

BLAST of Lcy10g017060 vs. ExPASy TrEMBL
Match: A0A0A0L685 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121740 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.5e-210
Identity = 389/516 (75.39%), Postives = 436/516 (84.50%), Query Frame = 0

Query: 10  CSSSSTCSSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNTPRTGLLRTTSVC 69
           C   + C SF S+K YTKISEY DRVLRP+ V+NK+YY+DESHS K     GL       
Sbjct: 3   CRRFAAC-SFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGL------- 62

Query: 70  RFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSKVDVTRD--VPAA 129
             HIP YGTH I T LPASS+K+WNTIPLT   +LSN R YSSAS  K D++ D  VPAA
Sbjct: 63  --HIPSYGTHRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAA 122

Query: 130 SSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELSPHADKLFDSHPY 189
           SSGVE DISNPPD+GRD LE+VKDVWQS VDA SFTGQKA+EVSDELSPH DKL DSHPY
Sbjct: 123 SSGVEPDISNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPY 182

Query: 190 LKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVEEIPYEKSFWGAL 249
           LKNVIVP+SMTL ATLLAW+VMPRLL+RFHKYSMR PVAI+  S+P EEIPYEKSFWGAL
Sbjct: 183 LKNVIVPVSMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGAL 242

Query: 250 EDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYRWKRNVLSRALAS 309
           EDPL YLVTFFA SQIG +VAPT++  EFV+QACRGAVILSLVWFIYRWK NVLSRALA+
Sbjct: 243 EDPLRYLVTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALAT 302

Query: 310 QTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIGGVTIAFAARDIR 369
           +TFAGL+R  LLTLDKVSSV LFAIGLM LAEASGVAVQSMLTVGGIGGV  AFAARDI 
Sbjct: 303 KTFAGLDRDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDIL 362

Query: 370 GNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSIVVPNSLLYGQEI 429
           GNILSGL M+FSQ FSLGDTIKAGAIEG+VVEMGLMTTSLLSAE+F +VVPNSL   Q I
Sbjct: 363 GNILSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVI 422

Query: 430 INESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEAPDCFPSQVESTW 489
           IN+SRAQ  AI K++PLQVDD+ST+SQI+D+IK+ML SHP+VFLGKEAP CF SQ+EST+
Sbjct: 423 INKSRAQWRAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTY 482

Query: 490 AELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           AELT+ CNL+KMGK+E FST+QDILLQS++IIK  G
Sbjct: 483 AELTLGCNLRKMGKDESFSTEQDILLQSVQIIKAKG 508

BLAST of Lcy10g017060 vs. NCBI nr
Match: KAG6586069.1 (Mechanosensitive ion channel protein 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 784.3 bits (2024), Expect = 7.0e-223
Identity = 412/528 (78.03%), Postives = 451/528 (85.42%), Query Frame = 0

Query: 1   MALFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNT 60
           M LFIL SI S+S +     SF   KSYTKISEY++RVLRPS V+NK+YY+DESHSLKN 
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNV 60

Query: 61  PRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSK 120
            RTG LR  SVC  H P YGTH  GT  PAS VK+WNTIP TSL +LSNPR YSS SS K
Sbjct: 61  SRTGFLRAPSVCCLHTPSYGTHPAGTFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120

Query: 121 VDVTRD--VPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 180
            D+T D  VP ASSGVEADISNPPD+GRDWLERVKD WQSVVDA SFTGQKAREVSDE S
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS 180

Query: 181 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 240
           PH DKL DSHPYLKN I P+ MTLTATLLAWVVMPRLLRRFHKYSMR PVAIL  S  +E
Sbjct: 181 PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPL YLVTF+A SQIG MVAPT++ SEFV+QACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 360
           WK NV SRALASQ F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 420
           GV  AFAARDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+V+EMGLMTTSLLSAE+F I
Sbjct: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 480
           VVPNSL   Q IIN+SRAQ  AITKKIP QV DLST+S+ISD+IKSML+SHP+VFLGKEA
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLKSHPKVFLGKEA 480

Query: 481 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+ES++AELTI CNLK+MGKEE FST+QDILLQS++I+KENG
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENG 528

BLAST of Lcy10g017060 vs. NCBI nr
Match: XP_022969688.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 783.1 bits (2021), Expect = 1.6e-222
Identity = 410/528 (77.65%), Postives = 450/528 (85.23%), Query Frame = 0

Query: 1   MALFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNT 60
           M LFIL SI S+S +     SF   KSYTKISEY++RVLRPS V+NK+YY+DESHSLKN 
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNV 60

Query: 61  PRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSK 120
            RTG +R  SVCR H P YGTH I T  PAS VK+WNTIP TSL +LSNPR YSS SS K
Sbjct: 61  SRTGFIRAPSVCRLHTPSYGTHPIATFSPASPVKYWNTIPPTSLAYLSNPRFYSSTSSRK 120

Query: 121 VDVTRD--VPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 180
            D+T D  VP ASSGVEADISNPPD+GRDWLERVKD WQS VDA SFTGQKAREVSDE S
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFS 180

Query: 181 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 240
           PH DK+ DSHPYLKN I P+ MTLTATLLAWVVMPRLLRRFHKYSMR PVAIL  S  +E
Sbjct: 181 PHVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPL YLVTF+A SQIG MVAPT++ SEFV+QACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 360
           WK NV SRALASQ F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 420
           GV  AFA+RDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+V+EMGLMTTSLLSAE+F I
Sbjct: 361 GVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 480
           VVPNSL   Q IIN+SRAQ  AITKKIP QV D ST+S+ISD+IKSMLRSHP+VFLGKEA
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEA 480

Query: 481 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+ES++AELTI CNLK+MGKEE FST+QDILLQS++IIKENG
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIIKENG 528

BLAST of Lcy10g017060 vs. NCBI nr
Match: XP_023538305.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 782.3 bits (2019), Expect = 2.6e-222
Identity = 410/528 (77.65%), Postives = 449/528 (85.04%), Query Frame = 0

Query: 1   MALFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNT 60
           M LFIL SI S+S +     SF   KSYTKISEY++RVLRPS V+NK+YY+DESHSLKN 
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNV 60

Query: 61  PRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSK 120
            RTG LR  SVCR H P YGTH   T  PAS VK+WNTIP TSL +LSNPR YSS SS K
Sbjct: 61  SRTGFLRAPSVCRLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120

Query: 121 VDVTRD--VPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 180
            D+T D  VP ASSGVEADISNPPD+GRDWLERVKD WQS VDA SFTGQKAREVSDE S
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFS 180

Query: 181 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 240
           PH DK+ DSHPYLKN I P+ MTLTATLLAWVVMPRLLRRFHKYSMR PVAIL  S  +E
Sbjct: 181 PHVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPL YLVTF+A SQIG MVAPT++ SEFV+QACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 360
           WK NV SRALASQ F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 420
           GV  AFAARDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+V+EMGLMTTSLLSAE+F I
Sbjct: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 480
           VVPNSL   Q IIN+SRAQ  AITKKIP QV D ST+S+ISD+IKSMLRSHP+VFLGKEA
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEA 480

Query: 481 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+ES++AELTI CNLK+MGKEE FST+QDILLQS++I+KENG
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENG 528

BLAST of Lcy10g017060 vs. NCBI nr
Match: XP_022937845.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 779.6 bits (2012), Expect = 1.7e-221
Identity = 412/528 (78.03%), Postives = 449/528 (85.04%), Query Frame = 0

Query: 1   MALFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNT 60
           M LFIL SI S+S +     SF   KSYTKISEY++RVLR S V+NK+YY+DESHSLKN 
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNV 60

Query: 61  PRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSK 120
            RTG LR  SVC  H P YGTH   T  PAS VK+WNTIP TSL +LSNPR YSS SS K
Sbjct: 61  SRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120

Query: 121 VDVTRD--VPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 180
            D+T D  VP ASSGVEADISNPPD+GRDWLERVKD WQSVVDA SFTGQKAREVSDE S
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS 180

Query: 181 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 240
           PH DKL DSHPYLKN I P+ MTLTATLLAWVVMPRLLRRFHKYSMR PVAIL  S  +E
Sbjct: 181 PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPL YLVTF+A SQIG MVAPT++ SEFV+QACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 360
           WK NV SRALASQ F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 420
           GV  AFAARDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+VVEMGLMTTSLLSAE+F I
Sbjct: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 480
           VVPNSL   Q IIN+SRAQ  AITKKIP QV DLST+S+ISD+IKSMLRSHP+VFLGKEA
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPKVFLGKEA 480

Query: 481 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+ES++AELTI CNLK+MGKEE FST+QDILLQS++I+KENG
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENG 528

BLAST of Lcy10g017060 vs. NCBI nr
Match: KAG7020846.1 (Mechanosensitive ion channel protein 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 777.7 bits (2007), Expect = 6.5e-221
Identity = 409/528 (77.46%), Postives = 449/528 (85.04%), Query Frame = 0

Query: 1   MALFILKSICSSSSTC---SSFHSYKSYTKISEYIDRVLRPSAVVNKKYYEDESHSLKNT 60
           M LFIL SI S+S +     SF   KSYTKISEY+++VLRPS V+NK+YY+DESHSLKN 
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNQVLRPSVVLNKQYYKDESHSLKNV 60

Query: 61  PRTGLLRTTSVCRFHIPIYGTHSIGTILPASSVKFWNTIPLTSLDHLSNPRLYSSASSSK 120
            RTG L   SVC  H P YGTH   T  PAS VK+WNTIP TSL +LSNPR YSS SS K
Sbjct: 61  SRTGFLGAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120

Query: 121 VDVTRD--VPAASSGVEADISNPPDLGRDWLERVKDVWQSVVDATSFTGQKAREVSDELS 180
            D+T D  VP ASSGVEADISNPPD+GRDWLERVKD WQSVVDA SFTGQKAREVSDE S
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS 180

Query: 181 PHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAIL--SIPVE 240
           PH DKL DSHPYLKN I P+ MTLTATLLAWVVMPRLLRRFHKYSMR PVAIL  S  +E
Sbjct: 181 PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEFVTQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPL YLVTF+A SQIG MVAPT++ SEFV+QACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMGLAEASGVAVQSMLTVGGIG 360
           WK NV SRALASQ F+GL+R+ LLTLDKVSSVGLFAIGLM LAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVTIAFAARDIRGNILSGLRMRFSQHFSLGDTIKAGAIEGKVVEMGLMTTSLLSAEQFSI 420
           GV  AFAARDI GN+LSGL M+FSQ FSLGDTIKAGAIEG+V+EMGLMTTSLLSAE+F I
Sbjct: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLLYGQEIINESRAQCHAITKKIPLQVDDLSTISQISDNIKSMLRSHPQVFLGKEA 480
           VVPNSL   Q IIN+SRAQ  AITKKIP QV DLST+S+ISD+IKSML+SHP+VFLGKEA
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLKSHPKVFLGKEA 480

Query: 481 PDCFPSQVESTWAELTISCNLKKMGKEEFFSTQQDILLQSIKIIKENG 522
           P CF SQ+ES++AELTI CNLK+MGKEE FST+QDILLQS++I+KENG
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENG 528

BLAST of Lcy10g017060 vs. TAIR 10
Match: AT4G00290.1 (Mechanosensitive ion channel protein )

HSP 1 Score: 488.4 bits (1256), Expect = 7.4e-138
Identity = 252/438 (57.53%), Postives = 333/438 (76.03%), Query Frame = 0

Query: 87  ASSVKFWNTI-PLTSLDHLSNPRLYSSASSSKVDVTRDVPAASSGVEADISNPPDLGRDW 146
           +S+++  N   P   L H S  R +SS S     +       +SGV     +    G DW
Sbjct: 55  SSNIRLHNDAKPFNYLGHSSYARAFSSKSDDFGSI------VASGVTGS-GDGNGNGNDW 114

Query: 147 LERVKDVWQSVVDATSFTGQKAREVSDELSPHADKLFDSHPYLKNVIVPLSMTLTATLLA 206
           +E+ KDV Q+ VDA + T +K ++VSDE+ PH  +  DS+PYLK+VIVP+S+T+T TL A
Sbjct: 115 VEKAKDVLQTSVDAVTETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFA 174

Query: 207 WVVMPRLLRRFHKYSMRGPVAILSIPV--EEIPYEKSFWGALEDPLLYLVTFFAVSQIGT 266
           WVVMPR+LRRFH Y+M+    +L +    E++PYEKSFWGALEDP  YLVTF A +QI  
Sbjct: 175 WVVMPRILRRFHTYAMQSSAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAA 234

Query: 267 MVAPTSMTSEFVTQACRGAVILSLVWFIYRWKRNVLSRALASQTFAGLNRKWLLTLDKVS 326
           MVAPT++ +++ +   +GAVILSLVWF+YRWK NV++R L++++F GL+R+ +LTLDKVS
Sbjct: 235 MVAPTTIAAQYFSPTVKGAVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVS 294

Query: 327 SVGLFAIGLMGLAEASGVAVQSMLTVGGIGGVTIAFAARDIRGNILSGLRMRFSQHFSLG 386
           SVGLFAIGLM  AEA GVAVQS+LTVGG+GGV  AFAARDI GN+LSGL M+FS+ FS+G
Sbjct: 295 SVGLFAIGLMASAEACGVAVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMG 354

Query: 387 DTIKAGAIEGKVVEMGLMTTSLLSAEQFSIVVPNSLLYGQEIINESRAQCHAITKKIPLQ 446
           DTIKAG++EG+V+EMGL TTSLL+AE+F ++VPNSL   Q I+N+SRAQ  AI  KIPLQ
Sbjct: 355 DTIKAGSVEGQVIEMGLTTTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQ 414

Query: 447 VDDLSTISQISDNIKSMLRSHPQVFLGKEAPDCFPSQVESTWAELTISCNLKKMGKEEFF 506
           +DDL  I QIS+ IK MLRS+ +VFLGKEAP C+ S+VE ++AELTI CNL +MGKEE +
Sbjct: 415 IDDLDMIPQISNEIKEMLRSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELY 474

Query: 507 STQQDILLQSIKIIKENG 522
           +TQQ++LL+++KIIK++G
Sbjct: 475 NTQQEVLLEAVKIIKKHG 485

BLAST of Lcy10g017060 vs. TAIR 10
Match: AT4G00234.1 (BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein (TAIR:AT4G00290.1); Has 57 Blast hits to 57 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 56; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 260.0 bits (663), Expect = 4.3e-69
Identity = 138/256 (53.91%), Postives = 183/256 (71.48%), Query Frame = 0

Query: 97  PLTSLDHLSNPRLYSSASSSKVDVTRDVPAASSGVEADISNPPDLGRDWLERVKDVWQSV 156
           P   L H S    Y+ A SSK D    + A+      D +     G DW+E+ KDV Q+ 
Sbjct: 16  PFNYLSHSS----YARAFSSKSDDFGSIVASGVTGSGDGN-----GNDWVEKAKDVVQTS 75

Query: 157 VDATSFTGQKAREVSDELSPHADKLFDSHPYLKNVIVPLSMTLTATLLAWVVMPRLLRRF 216
           V+A + T +K ++VSDE+ PH  +  DS+PYLK+VIVP+S+T+T TL AW+VMPR+LRRF
Sbjct: 76  VNAVAETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTVTGTLFAWIVMPRILRRF 135

Query: 217 HKYSMRGPVAILSIPV--EEIPYEKSFWGALEDPLLYLVTFFAVSQIGTMVAPTSMTSEF 276
           H Y+M+    +L +    E+IPYEKSFWGALEDP  YLVTF A +QI  MVAPT++ +++
Sbjct: 136 HTYAMQSSAKLLPVGFSNEDIPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQY 195

Query: 277 VTQACRGAVILSLVWFIYRWKRNVLSRALASQTFAGLNRKWLLTLDKVSSVGLFAIGLMG 336
            +   +GAVILS+VWF+YRWK NV++R L++++F GL+R  +LTLDKVSSVGLFAIGLM 
Sbjct: 196 FSPTVKGAVILSVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLDKVSSVGLFAIGLMA 255

Query: 337 LAEASGVAVQSMLTVG 351
            AEA GVAVQS+LTVG
Sbjct: 256 SAEACGVAVQSILTVG 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZL41.0e-13657.53Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX... [more]
P0AEB61.5e-1824.66Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB51.5e-1824.66Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
O251701.3e-1424.91Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC ... [more]
Q9ZKG71.7e-1424.91Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 ... [more]
Match NameE-valueIdentityDescription
A0A6J1I1Q47.5e-22377.65mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima O... [more]
A0A6J1FHY58.3e-22278.03mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita moschata... [more]
A0A6J1D2803.4e-21576.52mechanosensitive ion channel protein 1, mitochondrial-like OS=Momordica charanti... [more]
A0A5A7U5P82.5e-21075.59Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0L6852.5e-21075.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121740 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6586069.17.0e-22378.03Mechanosensitive ion channel protein 1, mitochondrial, partial [Cucurbita argyro... [more]
XP_022969688.11.6e-22277.65mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita maxima][more]
XP_023538305.12.6e-22277.65mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita pepo subsp... [more]
XP_022937845.11.7e-22178.03mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita moschata][more]
KAG7020846.16.5e-22177.46Mechanosensitive ion channel protein 1, mitochondrial [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
AT4G00290.17.4e-13857.53Mechanosensitive ion channel protein [more]
AT4G00234.14.3e-6953.91BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.287.1260coord: 274..376
e-value: 7.7E-12
score: 47.3
NoneNo IPR availablePANTHERPTHR30566YNAI-RELATED MECHANOSENSITIVE ION CHANNELcoord: 88..521
NoneNo IPR availablePANTHERPTHR30566:SF5MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATEDcoord: 88..521
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 380..427
e-value: 1.3E-10
score: 43.1
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 321..521
e-value: 1.3E-31
score: 109.8
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 364..428
IPR011014Mechanosensitive ion channel MscS, transmembrane-2SUPERFAMILY82861Mechanosensitive channel protein MscS (YggB), transmembrane regioncoord: 279..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g017060.1Lcy10g017060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane