Lcy10g014960 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g014960
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionCalcium-binding EF-hand
LocationChr10: 43191588 .. 43194503 (+)
RNA-Seq ExpressionLcy10g014960
SyntenyLcy10g014960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGTCAAAGAAACCCATAATGGCATCTCCACTTTAGACCAATGCGCAAACCAGTTCCATTTTCCTTTTCTCTTTTTCTTTTGTGTTTCCCTTAATACTTTATGTCGCCCATTTCCTCCGCTGAAATTTGCAAGAGCTTTCTTCTCTGTGTTTCCATGGAAGTTTCCCCTGGTGAGTACAACTTTCGGCTTTATAGTTCACGTCTTCTTGTTCTTGCTACTTGTTTCTCATTCAATTCATCGCCAGAGGAAGAAACGGAGATTTTTTGTTTCCCTGTACTTGCGCGTAAGAGAATTTAATATGTTCCTCGTTTGGGAATCTATTTTGCGTGAAATTTTCAGTTTGAGTTTCGAAGTTTGTTGTGTGTTCAAATAAAATTGTCTGCTCGTTGAATGTTGAGCGTCGTTTGCGTCGATTTTGATGTTAATCGTGCCGATTGGATTGACGGTGCAATTTAGGGGTTAACTTCGATAATCAATAGCCATTTTGATGATTGCTTTGTATTTACGCTAAGTTAATCTTGGAATCTGTTGTGAACGTATACCTGGTTGAAACCCCAAACTGAATTGTAAAGGCAAAAGTTTGAGGGTAAGAAAGATGCCAAAGGATAGATAGCTTGAAATTTGGGAAGAAGGCGATTTACAAGAAGCTGTGCAGTCTAGAAATTGGAGGAAATCTTGTGTCTGGGGCTTTGAGAAAGGATGGATAACTGGAAATTTGTTCTACTACTTGCTAGTCGGAACTTGGAAGAATCAAGTTCTTTTTAGGTTTTTTTCTTTACTAAGCAATGACCTGTAAGAAACTTTGGAGCATATCCAAAGTACATTTCCGGGGTTTAAAGGAAATATGAAGTAAATGATTAGTGAAAGAGAGGAAACTACTTCAGAAATGCTTCCATATATAGCATTTTTGTTTCCATTTTTATAGACAAGTAACAATAAACATTACAACTTGTATTGTTCTTTAAATGCTTATTTTCCATTCTCATGTCTTTTTTTGGCTGTTTGATATCTTCTGGAATTGCTATCTTGCTCATAGGATGCACTGATCTTGTTTTCATTTGATTTGTCAGGCACACTTTGATTTTCTCTATCTTTTATTCTAAAGGAACATCTCATTCCTGGCCTTCTGTTCAAAGTTGGAAGCATGTTTTTCTGCTGCAGTTCATCAAATAAATATAAGAGATTGGATGCAAAGCTTGGAAGGAAAATGGTTGAAGTCAAAAGAAATTTAGCTGGACATGACAATTTCAAGTCATTAAATGGGATAATCTTAAGATTTCCCCAATTCAAAGAGGGATTACAAAACATCAGAGGTGTATTTGAACAGTATGGTGAGCTTCATTTTAGCTGCATCAACTCTTTTTGAGCTTCTCCTGCTCAAGATTTTCTCACATACTCTCTCTTTCTTTCTCTCACAGATGAAGATTCCAATGGAAGCATTGATCGCGAAGAACTCAAAAAATGCTTAAAACAGCTGCAAATGCATATGACCGAAGAAGAAGTCGAGGATCTTTTCCACTCTTGTGACATTGATGGAAGTGCTGGAATACAATTCAACGAGTTTATTGTTCTCCTATGTCTCATTTATCTCCTCAAGGACCAGCATTCGCCTACTGTACGCACAAACAAACTTTTATCCCTCCATTTTCGTTCAGTTTTCAATCTGTTTATCATTCTTGGGCATAACGTAACCATTCTAGAGGTTTATTACAGAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACCTTTGACACAATCATCCAAGCTTTCATATTTCTCGATAAAAATGGCGATGGCAAGCTCAACAAAAAAGAAATGGTCAAGGCTCTGAATGAGGCTTCTCCATGCGAGAGATCTCCTGCACGCATTACACAGACTAGATTCAGTATTGCTCCCTTCACTAAAGATACTCCATATCTGTTTTTGTTTAACGAATCGTTCTAGAAAGTTCAAATATGTCGAACATATCTGATTAAAGTCAAATACATATTCATTCGAGCTAAACACTATTTTTCACCTATAAGTTTCGTTTTTTTGTTATCTACTTTTAGAAGATTTTAAAAATCCAACCATCAAATTTGAAAACTTAAAAAAGTAGTTTATAAAAACATGTTCTTGTTTTTAGAATTTGGCTAAGAATTCAAACTTTTCATCCAGAAATGTGAAAATTATAGTAAAGAAATTATGAGGAAATAAATACAACTTTCAAAAACATAAAACTAAAAACCAAAGTCTCGTTTGATAACCATTTGATTTTTGAAAATTATGCTTGTTTTCTCCAAATTTTATTACAATGGTTTTCGTCTTTGTTGATGAAATATTTGAATTCTTAACCAAATTCCAAAAACAGAAACAAGTTTTCGAAAACTACTTGAGGTGGAAGTAGTGTTTATATTTAGTTTTCAAAAACCAAATATTTATCAAACGGGTCGAAATTATTATCAAATATTCTTAAACTTTCACCTATTTGAACTGTTCTGCTCTTCTATTATGTATTGATATTGATTTCCCTAGTTTTCTTAATGGATGCAGAAGAAATGGATTGGAACAACAGTGGAAAGGTCAATTTCAGGGAGTTTCTATTTGGTTTCATTAACTGGGTTGGAATTGACACTGATGATGACATTTCTCTTACATGAAGTTCAAACAGGACAGAGGACATCAAGACAGACATGAAGACATTGACTTAAAAAATAAGGTCAGACTGATAAGTCGTATTCACAACTGAAACACTTGCTGATGATTATAGTGAGATACTACTCTTGTTTGTTTATTTCATTTGGCGAATTGTAATGTTCGGACAACCTTGTGAATTAGATTACATAATGCTATTCTTATAATATCGTCGTTGTTGTTACTATATCGACTCGTAGAATTTGATACAGTGAAATCTAGCTCGAGAGAGTTTATA

mRNA sequence

CTTGTCAAAGAAACCCATAATGGCATCTCCACTTTAGACCAATGCGCAAACCAGTTCCATTTTCCTTTTCTCTTTTTCTTTTGTGTTTCCCTTAATACTTTATGTCGCCCATTTCCTCCGCTGAAATTTGCAAGAGCTTTCTTCTCTGTGTTTCCATGGAAGTTTCCCCTGGCACACTTTGATTTTCTCTATCTTTTATTCTAAAGGAACATCTCATTCCTGGCCTTCTGTTCAAAGTTGGAAGCATGTTTTTCTGCTGCAGTTCATCAAATAAATATAAGAGATTGGATGCAAAGCTTGGAAGGAAAATGGTTGAAGTCAAAAGAAATTTAGCTGGACATGACAATTTCAAGTCATTAAATGGGATAATCTTAAGATTTCCCCAATTCAAAGAGGGATTACAAAACATCAGAGGTGTATTTGAACAGTATGATGAAGATTCCAATGGAAGCATTGATCGCGAAGAACTCAAAAAATGCTTAAAACAGCTGCAAATGCATATGACCGAAGAAGAAGTCGAGGATCTTTTCCACTCTTGTGACATTGATGGAAGTGCTGGAATACAATTCAACGAGTTTATTGTTCTCCTATGTCTCATTTATCTCCTCAAGGACCAGCATTCGCCTACTAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACCTTTGACACAATCATCCAAGCTTTCATATTTCTCGATAAAAATGGCGATGGCAAGCTCAACAAAAAAGAAATGGTCAAGGCTCTGAATGAGGCTTCTCCATGCGAGAGATCTCCTGCACGCATTACACAGACTAGATTCAAAGAAATGGATTGGAACAACAGTGGAAAGGTCAATTTCAGGGAGTTTCTATTTGGTTTCATTAACTGGGTTGGAATTGACACTGATGATGACATTTCTCTTACATGAAGTTCAAACAGGACAGAGGACATCAAGACAGACATGAAGACATTGACTTAAAAAATAAGGTCAGACTGATAAGTCGTATTCACAACTGAAACACTTGCTGATGATTATAGTGAGATACTACTCTTGTTTGTTTATTTCATTTGGCGAATTGTAATGTTCGGACAACCTTGTGAATTAGATTACATAATGCTATTCTTATAATATCGTCGTTGTTGTTACTATATCGACTCGTAGAATTTGATACAGTGAAATCTAGCTCGAGAGAGTTTATA

Coding sequence (CDS)

ATGTTTTTCTGCTGCAGTTCATCAAATAAATATAAGAGATTGGATGCAAAGCTTGGAAGGAAAATGGTTGAAGTCAAAAGAAATTTAGCTGGACATGACAATTTCAAGTCATTAAATGGGATAATCTTAAGATTTCCCCAATTCAAAGAGGGATTACAAAACATCAGAGGTGTATTTGAACAGTATGATGAAGATTCCAATGGAAGCATTGATCGCGAAGAACTCAAAAAATGCTTAAAACAGCTGCAAATGCATATGACCGAAGAAGAAGTCGAGGATCTTTTCCACTCTTGTGACATTGATGGAAGTGCTGGAATACAATTCAACGAGTTTATTGTTCTCCTATGTCTCATTTATCTCCTCAAGGACCAGCATTCGCCTACTAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACCTTTGACACAATCATCCAAGCTTTCATATTTCTCGATAAAAATGGCGATGGCAAGCTCAACAAAAAAGAAATGGTCAAGGCTCTGAATGAGGCTTCTCCATGCGAGAGATCTCCTGCACGCATTACACAGACTAGATTCAAAGAAATGGATTGGAACAACAGTGGAAAGGTCAATTTCAGGGAGTTTCTATTTGGTTTCATTAACTGGGTTGGAATTGACACTGATGATGACATTTCTCTTACATGA

Protein sequence

MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYLLKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISLT
Homology
BLAST of Lcy10g014960 vs. ExPASy Swiss-Prot
Match: Q9LRN6 (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.3e-66
Identity = 126/224 (56.25%), Postives = 165/224 (73.66%), Query Frame = 0

Query: 1   MFFCCSSS---NKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRG 60
           M  CC +S    KY  LDAKL RKMVE +    GH + KS++ II++FP+ +EGL+NIR 
Sbjct: 1   MLCCCVNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRS 60

Query: 61  VFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCL 120
           VFE YD D+NG+ID EELKKCL++L++ +++EEV+ L+  CD+DGS GIQFNEFIVLLCL
Sbjct: 61  VFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCL 120

Query: 121 IYLL--KDQHSPTKSKLGSPQL-EATFDTIIQAFIFLDKNGDGKLNKKEMVKAL-NEASP 180
           IYLL      S T+S+   P+L E+ FD I++ F+FLDK+G GKLNK +++K L NE  P
Sbjct: 121 IYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYP 180

Query: 181 CERSPARITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDD 218
            ERSP+ +T  RF+EMDW   GKV FREFLF F++WVG+D  DD
Sbjct: 181 LERSPSHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADD 224

BLAST of Lcy10g014960 vs. ExPASy Swiss-Prot
Match: Q52K82 (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 4.8e-56
Identity = 102/206 (49.51%), Postives = 149/206 (72.33%), Query Frame = 0

Query: 15  DAKLGRKMVE-VKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRE 74
           + KL  K++E V+R  +     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 75  ELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYLLKDQHSPTKSK-- 134
           ELK C+++L++   EEE+ DLF +CDI+   GI F EFIVLLCL+YLLKD  S  + K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 135 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARITQTRFKEMDW 194
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E+   ERS  RI   RF+EMDW
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG--ERSSGRIAMKRFEEMDW 197

Query: 195 NNSGKVNFREFLFGFINWVGIDTDDD 218
           + +G VNF+EFLF F  WVGID +++
Sbjct: 198 DKNGMVNFKEFLFAFTQWVGIDENEE 221

BLAST of Lcy10g014960 vs. ExPASy Swiss-Prot
Match: P41210 (Caltractin OS=Atriplex nummularia OX=3553 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.2e-12
Identity = 45/151 (29.80%), Postives = 74/151 (49.01%), Query Frame = 0

Query: 53  QNIRGVFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFI 112
           Q I+  FE +D D +G+ID +EL   ++ L   MTEE++  +    D DGS  I F+EF 
Sbjct: 25  QEIKEAFELFDTDGSGTIDAKELNVAMRALGFEMTEEQINQMIADVDKDGSGAIDFDEF- 84

Query: 113 VLLCLIYLLKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEAS 172
              C +   K     TK +L            ++AF  +D++ +GK++ +++ +   E  
Sbjct: 85  ---CHMMTAKIGERDTKEEL------------MKAFRIIDQDNNGKISPEDIQRIAKELG 144

Query: 173 PCERSPARITQTRFKEMDWNNSGKVNFREFL 204
             E    +  Q   +E D +  G+VN  EFL
Sbjct: 145 --ENFTVKDIQDMIEEADRDRDGEVNVEEFL 157

BLAST of Lcy10g014960 vs. ExPASy Swiss-Prot
Match: Q9ZSA3 (Calcium-dependent protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CPK22 PE=3 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-11
Identity = 46/155 (29.68%), Postives = 78/155 (50.32%), Query Frame = 0

Query: 49  KEGLQNIRGVFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQF 108
           +E ++ ++ +FE  D D +GSI  EELK  L +    ++E EV+ L  + D+DG+  I +
Sbjct: 345 EEEIKGLKTMFENMDMDKSGSITYEELKMGLNRHGSKLSETEVKQLMEAADVDGNGTIDY 404

Query: 109 NEFIVLLCLIYLL-KDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKA 168
            EFI      + L +D+H                  + +AF + DK+G G + K+E+  A
Sbjct: 405 IEFISATMHRHRLERDEH------------------LYKAFQYFDKDGSGHITKEEVEIA 464

Query: 169 LNEASPCERSPARITQTRFKEMDWNNSGKVNFREF 203
           + E    + + A+       E D NN GK+++ EF
Sbjct: 465 MKEHGMGDEANAK---DLISEFDKNNDGKIDYEEF 478

BLAST of Lcy10g014960 vs. ExPASy Swiss-Prot
Match: P02589 (Troponin C, skeletal muscle OS=Pelophylax lessonae OX=45623 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 42/155 (27.10%), Postives = 74/155 (47.74%), Query Frame = 0

Query: 49  KEGLQNIRGVFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQF 108
           +E +   +  F+ +D D  G I  +EL   ++ L    T+EE++ +    D DGS  I F
Sbjct: 16  EEMIAEFKAAFDMFDTDGGGDISTKELGTVMRMLGQTPTKEELDAIIEEVDEDGSGTIDF 75

Query: 109 NEFIVLLCLIYLLKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKAL 168
            EF+V++ +  + +D    ++ +L             + F   DKN DG ++ +E+ + L
Sbjct: 76  EEFLVMM-VRQMKEDAQGKSEEELA------------ECFRIFDKNADGYIDSEELGEIL 135

Query: 169 NEASPCERSPARITQTRFKEMDWNNSGKVNFREFL 204
             +   E       +   K+ D NN GK++F EFL
Sbjct: 136 RSSG--ESITDEEIEELMKDGDKNNDGKIDFDEFL 155

BLAST of Lcy10g014960 vs. ExPASy TrEMBL
Match: A0A0A0L6R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130890 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 2.9e-117
Identity = 209/221 (94.57%), Postives = 216/221 (97.74%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           +FFCCSSSNKY RLDAKLGRKMVEVKRN AGHDNFKSLNGIILRFPQFKEGLQNIRGVFE
Sbjct: 7   IFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL+QLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKD+HS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP ERSPAR
Sbjct: 127 LKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPYERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISLT 222
           IT+TRFKEMDWNNSGKVNFREFLFGFINWVG+DTDDD+S T
Sbjct: 187 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLSPT 227

BLAST of Lcy10g014960 vs. ExPASy TrEMBL
Match: A0A6J1EBV7 (probable calcium-binding protein CML22 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431775 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 3.9e-117
Identity = 207/220 (94.09%), Postives = 215/220 (97.73%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           MF CCSSSNKY R+D KLGRKMVEVKRNLAGHDNFKSLNGIILRFPQF+EGLQNIRGVFE
Sbjct: 7   MFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQNIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL+QLQMHMTE+EVEDLFHSCDIDGS+GIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSSGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP ERSPAR
Sbjct: 127 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPFERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISL 221
           IT+TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDD+SL
Sbjct: 187 ITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 226

BLAST of Lcy10g014960 vs. ExPASy TrEMBL
Match: A0A6J1ITH1 (probable calcium-binding protein CML22 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479770 PE=4 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 6.6e-117
Identity = 207/221 (93.67%), Postives = 215/221 (97.29%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           MF CCSSSNKY R+D KLGRKMVEVKRNLAGHDNFKSLNGIILRFPQF+EGLQNIRGVF 
Sbjct: 7   MFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQNIRGVFA 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL+QLQMHMTE+EVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP ERSPAR
Sbjct: 127 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPFERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISLT 222
           IT+TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDD+SL+
Sbjct: 187 ITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLS 227

BLAST of Lcy10g014960 vs. ExPASy TrEMBL
Match: A0A1S3AVV2 (probable calcium-binding protein CML22 OS=Cucumis melo OX=3656 GN=LOC103483480 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.5e-116
Identity = 207/219 (94.52%), Postives = 215/219 (98.17%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           +FFCCSSSNKY RLDAKLGRKMVEVKRN AGHDNFKSLNGIILRFPQFKEGLQNIRGVFE
Sbjct: 7   IFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL++LQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQKLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKD+HS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP ERSPAR
Sbjct: 127 LKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPYERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDIS 220
           IT+TRFKEMDWNNSGKVNFREFLFGFINWVG+DTDDD+S
Sbjct: 187 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLS 225

BLAST of Lcy10g014960 vs. ExPASy TrEMBL
Match: A0A6J1G1T7 (probable calcium-binding protein CML22 OS=Cucurbita moschata OX=3662 GN=LOC111449869 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 8.9e-114
Identity = 204/221 (92.31%), Postives = 213/221 (96.38%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           MFFCCSSSNKYKRLD KLGRKM+EVK+NLAGHDNFKSLNGIILRFPQFKEGLQ+IRGVFE
Sbjct: 7   MFFCCSSSNKYKRLDEKLGRKMLEVKKNLAGHDNFKSLNGIILRFPQFKEGLQDIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSID EELKKCL+QLQMHMTEEEV+DLFHSCDIDGSAGIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDPEELKKCLQQLQMHMTEEEVKDLFHSCDIDGSAGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKDQH P  SKLGSP+L ATFDTIIQAFIFLDKNGDGKLNKKEMVKALN  +PCERSPAR
Sbjct: 127 LKDQHLPNISKLGSPRLAATFDTIIQAFIFLDKNGDGKLNKKEMVKALN--APCERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISLT 222
           ITQTRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDD+SLT
Sbjct: 187 ITQTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLT 225

BLAST of Lcy10g014960 vs. NCBI nr
Match: XP_011650876.1 (probable calcium-binding protein CML22 [Cucumis sativus] >XP_031737636.1 probable calcium-binding protein CML22 [Cucumis sativus] >KGN56734.1 hypothetical protein Csa_010293 [Cucumis sativus])

HSP 1 Score: 431.0 bits (1107), Expect = 6.1e-117
Identity = 209/221 (94.57%), Postives = 216/221 (97.74%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           +FFCCSSSNKY RLDAKLGRKMVEVKRN AGHDNFKSLNGIILRFPQFKEGLQNIRGVFE
Sbjct: 7   IFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL+QLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKD+HS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP ERSPAR
Sbjct: 127 LKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPYERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISLT 222
           IT+TRFKEMDWNNSGKVNFREFLFGFINWVG+DTDDD+S T
Sbjct: 187 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLSPT 227

BLAST of Lcy10g014960 vs. NCBI nr
Match: XP_022924247.1 (probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_022924248.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_022924249.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_022924250.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_023526231.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo] >XP_023526232.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo] >XP_023526233.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo] >XP_023526234.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 430.6 bits (1106), Expect = 8.0e-117
Identity = 207/220 (94.09%), Postives = 215/220 (97.73%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           MF CCSSSNKY R+D KLGRKMVEVKRNLAGHDNFKSLNGIILRFPQF+EGLQNIRGVFE
Sbjct: 7   MFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQNIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL+QLQMHMTE+EVEDLFHSCDIDGS+GIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSSGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP ERSPAR
Sbjct: 127 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPFERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISL 221
           IT+TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDD+SL
Sbjct: 187 ITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 226

BLAST of Lcy10g014960 vs. NCBI nr
Match: XP_022980390.1 (probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_022980391.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_022980392.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_022980393.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima])

HSP 1 Score: 429.9 bits (1104), Expect = 1.4e-116
Identity = 207/221 (93.67%), Postives = 215/221 (97.29%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           MF CCSSSNKY R+D KLGRKMVEVKRNLAGHDNFKSLNGIILRFPQF+EGLQNIRGVF 
Sbjct: 7   MFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQNIRGVFA 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL+QLQMHMTE+EVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP ERSPAR
Sbjct: 127 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPFERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISLT 222
           IT+TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDD+SL+
Sbjct: 187 ITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLS 227

BLAST of Lcy10g014960 vs. NCBI nr
Match: KAG6582474.1 (putative calcium-binding protein CML22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 428.7 bits (1101), Expect = 3.0e-116
Identity = 206/220 (93.64%), Postives = 214/220 (97.27%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           MF CCSSSNKY R+D KLGRKMVEVKRNLAGHDNFKSLNGIILRFPQF+EGLQNIRGVFE
Sbjct: 7   MFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQNIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL+QLQMHM E+EVEDLFHSCDIDGS+GIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQQLQMHMNEDEVEDLFHSCDIDGSSGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP ERSPAR
Sbjct: 127 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPFERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDISL 221
           IT+TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDD+SL
Sbjct: 187 ITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 226

BLAST of Lcy10g014960 vs. NCBI nr
Match: XP_008438356.1 (PREDICTED: probable calcium-binding protein CML22 [Cucumis melo] >XP_008438357.1 PREDICTED: probable calcium-binding protein CML22 [Cucumis melo])

HSP 1 Score: 428.7 bits (1101), Expect = 3.0e-116
Identity = 207/219 (94.52%), Postives = 215/219 (98.17%), Query Frame = 0

Query: 1   MFFCCSSSNKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60
           +FFCCSSSNKY RLDAKLGRKMVEVKRN AGHDNFKSLNGIILRFPQFKEGLQNIRGVFE
Sbjct: 7   IFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 66

Query: 61  QYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 120
           QYDEDSNGSIDREELKKCL++LQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL
Sbjct: 67  QYDEDSNGSIDREELKKCLQKLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYL 126

Query: 121 LKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPAR 180
           LKD+HS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP ERSPAR
Sbjct: 127 LKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPYERSPAR 186

Query: 181 ITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDIS 220
           IT+TRFKEMDWNNSGKVNFREFLFGFINWVG+DTDDD+S
Sbjct: 187 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLS 225

BLAST of Lcy10g014960 vs. TAIR 10
Match: AT3G24110.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 254.2 bits (648), Expect = 9.5e-68
Identity = 126/224 (56.25%), Postives = 165/224 (73.66%), Query Frame = 0

Query: 1   MFFCCSSS---NKYKRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRG 60
           M  CC +S    KY  LDAKL RKMVE +    GH + KS++ II++FP+ +EGL+NIR 
Sbjct: 1   MLCCCVNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRS 60

Query: 61  VFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCL 120
           VFE YD D+NG+ID EELKKCL++L++ +++EEV+ L+  CD+DGS GIQFNEFIVLLCL
Sbjct: 61  VFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCL 120

Query: 121 IYLL--KDQHSPTKSKLGSPQL-EATFDTIIQAFIFLDKNGDGKLNKKEMVKAL-NEASP 180
           IYLL      S T+S+   P+L E+ FD I++ F+FLDK+G GKLNK +++K L NE  P
Sbjct: 121 IYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYP 180

Query: 181 CERSPARITQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDD 218
            ERSP+ +T  RF+EMDW   GKV FREFLF F++WVG+D  DD
Sbjct: 181 LERSPSHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADD 224

BLAST of Lcy10g014960 vs. TAIR 10
Match: AT4G26470.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 219.2 bits (557), Expect = 3.4e-57
Identity = 102/206 (49.51%), Postives = 149/206 (72.33%), Query Frame = 0

Query: 15  DAKLGRKMVE-VKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRE 74
           + KL  K++E V+R  +     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 75  ELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYLLKDQHSPTKSK-- 134
           ELK C+++L++   EEE+ DLF +CDI+   GI F EFIVLLCL+YLLKD  S  + K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 135 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARITQTRFKEMDW 194
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E+   ERS  RI   RF+EMDW
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG--ERSSGRIAMKRFEEMDW 197

Query: 195 NNSGKVNFREFLFGFINWVGIDTDDD 218
           + +G VNF+EFLF F  WVGID +++
Sbjct: 198 DKNGMVNFKEFLFAFTQWVGIDENEE 221

BLAST of Lcy10g014960 vs. TAIR 10
Match: AT4G26470.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 175.3 bits (443), Expect = 5.6e-44
Identity = 84/175 (48.00%), Postives = 124/175 (70.86%), Query Frame = 0

Query: 15  DAKLGRKMVE-VKRNLAGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRE 74
           + KL  K++E V+R  +     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 75  ELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLLCLIYLLKDQHSPTKSK-- 134
           ELK C+++L++   EEE+ DLF +CDI+   GI F EFIVLLCL+YLLKD  S  + K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 135 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARITQTRF 187
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E+   ERS  RI   RF
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG--ERSSGRIAMKRF 190

BLAST of Lcy10g014960 vs. TAIR 10
Match: AT4G04710.1 (calcium-dependent protein kinase 22 )

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-11
Identity = 47/157 (29.94%), Postives = 78/157 (49.68%), Query Frame = 0

Query: 49  KEGLQNIRGVFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFH--SCDIDGSAGI 108
           +E ++ ++ +FE  D D +GSI  EELK  L +    ++E EV+ L    S D+DG+  I
Sbjct: 331 EEEIKGLKTMFENMDMDKSGSITYEELKMGLNRHGSKLSETEVKQLMEAVSADVDGNGTI 390

Query: 109 QFNEFIVLLCLIYLL-KDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMV 168
            + EFI      + L +D+H                  + +AF + DK+G G + K+E+ 
Sbjct: 391 DYIEFISATMHRHRLERDEH------------------LYKAFQYFDKDGSGHITKEEVE 450

Query: 169 KALNEASPCERSPARITQTRFKEMDWNNSGKVNFREF 203
            A+ E    + + A+       E D NN GK+++ EF
Sbjct: 451 IAMKEHGMGDEANAK---DLISEFDKNNDGKIDYEEF 466

BLAST of Lcy10g014960 vs. TAIR 10
Match: AT2G41100.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.8e-11
Identity = 43/154 (27.92%), Postives = 74/154 (48.05%), Query Frame = 0

Query: 56  RGVFEQYDEDSNGSIDREELKKCLKQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVLL 115
           R  F  +D++ +GSI ++EL+  +  L  + T+ +++D+ +  D+DG   I F EF+ L+
Sbjct: 103 RESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTIDFPEFLYLM 162

Query: 116 CLIYLLKDQ---HSPTKSKLGSPQLEATFDTII---QAFIFLDKNGDGKLNKKEMVKALN 175
                 K+Q    +P  +K      + T D I+   +AF   DKNGDG +   E+   + 
Sbjct: 163 A-----KNQGHDQAPRHTKKTMVDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTMR 222

Query: 176 EASPCERSPARITQTRFKEMDWNNSGKVNFREFL 204
                E       Q    E D +  G ++F EF+
Sbjct: 223 SLG--ETQTKAELQDMINEADADGDGTISFSEFV 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LRN61.3e-6656.25Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 ... [more]
Q52K824.8e-5649.51Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 ... [more]
P412107.2e-1229.80Caltractin OS=Atriplex nummularia OX=3553 PE=2 SV=1[more]
Q9ZSA31.6e-1129.68Calcium-dependent protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CPK22 PE=... [more]
P025891.4e-1027.10Troponin C, skeletal muscle OS=Pelophylax lessonae OX=45623 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L6R72.9e-11794.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130890 PE=4 SV=1[more]
A0A6J1EBV73.9e-11794.09probable calcium-binding protein CML22 isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1ITH16.6e-11793.67probable calcium-binding protein CML22 isoform X1 OS=Cucurbita maxima OX=3661 GN... [more]
A0A1S3AVV21.5e-11694.52probable calcium-binding protein CML22 OS=Cucumis melo OX=3656 GN=LOC103483480 P... [more]
A0A6J1G1T78.9e-11492.31probable calcium-binding protein CML22 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
XP_011650876.16.1e-11794.57probable calcium-binding protein CML22 [Cucumis sativus] >XP_031737636.1 probabl... [more]
XP_022924247.18.0e-11794.09probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_02292... [more]
XP_022980390.11.4e-11693.67probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_0229803... [more]
KAG6582474.13.0e-11693.64putative calcium-binding protein CML22, partial [Cucurbita argyrosperma subsp. s... [more]
XP_008438356.13.0e-11694.52PREDICTED: probable calcium-binding protein CML22 [Cucumis melo] >XP_008438357.1... [more]
Match NameE-valueIdentityDescription
AT3G24110.19.5e-6856.25Calcium-binding EF-hand family protein [more]
AT4G26470.13.4e-5749.51Calcium-binding EF-hand family protein [more]
AT4G26470.25.6e-4448.00Calcium-binding EF-hand family protein [more]
AT4G04710.11.6e-1129.94calcium-dependent protein kinase 22 [more]
AT2G41100.14.8e-1127.92Calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 72..92
NoneNo IPR availableGENE3D1.10.238.10coord: 18..115
e-value: 2.3E-19
score: 71.1
NoneNo IPR availableGENE3D1.10.238.10coord: 116..210
e-value: 7.6E-12
score: 47.0
NoneNo IPR availablePANTHERPTHR23064TROPONINcoord: 1..219
NoneNo IPR availablePANTHERPTHR23064:SF53CALCIUM-BINDING PROTEIN CML22-RELATEDcoord: 1..219
IPR002048EF-hand domainSMARTSM00054efh_1coord: 181..209
e-value: 13.0
score: 8.6
coord: 143..171
e-value: 0.027
score: 23.6
coord: 54..82
e-value: 0.0049
score: 26.1
coord: 90..118
e-value: 2.6
score: 14.4
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 142..203
e-value: 8.2E-8
score: 32.6
coord: 54..113
e-value: 6.1E-11
score: 42.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 86..121
score: 9.603815
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 139..174
score: 10.663839
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 50..85
score: 13.676544
IPR002048EF-hand domainCDDcd00051EFhcoord: 55..115
e-value: 1.69685E-14
score: 63.7209
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 63..75
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 152..164
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 50..216

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g014960.1Lcy10g014960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding