Lcy10g014600 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g014600
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionGDT1 family protein
LocationChr10: 42794323 .. 42797711 (-)
RNA-Seq ExpressionLcy10g014600
SyntenyLcy10g014600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGATTGGTTGTTCGGTCGCCGTTCGTTTCAACGGGGAAGAAGCTTCCCTTCTCGCCTTTGCTCGATTCTTTGCCAAGTTCTTCTGCTTCTGGCAGTGTATTCGCGAAGGCCGTATCAACATCGCGTAATCTCCTCTTGCATTTCCTCTTGTTAATTTTCTGGTTTTGCTTTTGATTTTGCAATTTCCTGAAAAGCTGAGCATCATGTGTAGATTAGTTAGTTACTCGTTGTTTAGGGAAACTGATTGTTTGAGTGTTTCTTCGAGTAATTTACGAATGATTATTTTGTTGACTGTCTTAATATTTTTGAATTGATATGCGTATTCCGTGCATTCTGTGAACGTATTGAGCGAGCAGTGAGTGTGTTGCTTACAGTGAAATGTAGAAGAGTGACGAGATTGAACGGGACGTATGGTAAAATCAGAGCACATTCGTCGAATGTAAGTATTGGATCCGATGGATACAGGCACGAGGAGGAAAAGGAGGGCCAGCATGTTATTTCCAGTAGTGCACCTGTTGATAGTAGTTCATCTAAAATGTGAGTTAAGTCATTTTTGTTTTGACGTTTCTGAGTTGAAATTGAAACTTATTGGTGTTGTATCAAATTATCGATTTCTGTGACTATGGCTTGGGTAATGCACAGTATACATTATTCTACAATTCCAATTGATTTTCCGGAGCAATGGTTATAAAATCTAATGTCTCGTCTACTGATATATTTGTTATCATCTAAAATCAATATTTCATTAATCTGGTTTTTGGGATCTCTATTCCATTAGATATTTTTGACGGAGTTGCGAAAAGTAAACAGTGACTTTTACCTGTAACCATGCTAACTTCTCCACTGAGTTGATATAGTCAACGACAACTTTGAAAATTTGTCGTGTAAAACTGATTCTCTCGCCTTGCGTTGTAATTTAGTGAATCCAGGACTTACTTGGCTCTCTCTTATGAACCAATATCTCTTCTTGTTCTTCTTTACTCATTTATGTATCTTCTATGTTTTGGCAGTGAGAAGTCTCCAAATGGATTGCCTTATCCTCTTTCTATTGCATTAGTACTAATTGGGTGCAGTTTAGTATTCTCGTTGATTGCCTTTGTGAAAGGAGGACCTTCATCAATCTTGGCAGCAGTTGCGAAGTCAGGGTTCACTGCTGCCTTCTCATTAATATTTGTCTCTGAAATTGGGGACAAGGTGACTTTTTTACATTGGAACTTCGTATATATCAAACCTGAGATTTCATCAATTCTATCCAGGTTCTCTAGTTTTGCCTAAGAGTTCAGAACCATATATTCATTGAATAAAATAAGATAAATAAAAGAAAATATATGCTATCAATCCACCGAGACAGGAAAGCTTGAAACTTGACCTTGTCTAACCACATTACAATTCAAAGAATTTCAAGTGAAGTAATCAAAATTTTCAAAATGTTCACAGTTATTTGTCTTTACTTCCTCCTGGTAAGTTGGCTTATACCTTATCTCTTTTGGACAGACGTTTTTCATTGCTGCACTCTTGGCCATGCAATATAAGAAAGGACTGGTATATCCTCTCGCACTTCTCTCTCCCTCTGCTTAAGTACTTTTGCAGTTGGTACCATGTTTTCTTTTTTCTTTTTCTTTTGTTCTCTATTACTTGATTCTGTGGGTTAGGAATTGAAAGTTCTATTCAGGTATTGAATTGTTGAACAGGTTCTGTTAGGATCTATGGGAGCACTTTCACTTATGACAGTCCTCTCAGTTATAATTGGACGGATATTTCACTCAGTACCTGCTCAATTCCAGACAAGTAAGCTGAAGCTATTTGGACTTTCTCAACTGCTCTAGAATTTGCGTCAATTTTGACTTAACTAGTACCAAGAGAATGTTGCTTGGTATTGTATTTTTTATTAGAATTAATGTAGTTTATTCTTTCTGCAATTTATCTGGCCACATAACAGAAACACTAGTGTAGTTGACGTTTTCCACAGCTTATGAATAACTTTTTTTTTTGGTCTCAACATCTATCAATATATCCAGTAATGGCTTTTAATCTCTTTGAACGTACTGTCTTCTTGCTCCTTTCTTTGTGAGGACAATAACTTTGGCCCTTCTTTCTCTATATAGTAGGATATGATTCTGCTGAGACAATCAACAACTTAATCCATATGTGCTGATATATAATATTAGCTTTATTGTTTGAAAAGTGTATTTTGTTTTATTAAACATTCAATTCTTTATTTGATATTTTCTAGTGTAACAAATAATACTTCTCCTAGACATAATTTTAGGTCCAAAATTCTAACGATATTTACTTGCTGTCATTTGTGTAGCTTTACCAATTGGAGAATATGCGGCAGTAACACTTCTGTTGTTCTTTGGTCTCAAGTCCATTAAAGATGCATGGGATCTTCCCTCGAGTGTTCCTAAACAAGGTGATGAGAGTGGCCCAGAACTAGATGAATATGTCGAAGCGGAGGAGCTTGTTAAAGAAAAGGTAGATCCCCAATGATAAGTTCCCTGTAGGTTTGGAATTTTGTTTTGACAAGGCTCTTTTGAAAACTATTGCTTCTTTGCTGAATAGTCCTCGGGTTGTATATTTCATATTTGTCTGACCCTGTGACTGAAAGTCTGACACATGCTCTTTCTGTCCACCTCTTACATGCAAATACACATGTAGCTAATTCTGCCTTGTGATAAACCTTTTATTGTTTTCAGGTTTCAAAACGGCTCTCAAATCCACTTGAAATCATCTGGAAGTCGTTCAGCCTTATATTTTTTGCTGTGAGTAGTTTTTAAATGAGTTCGTGTGTAGTTTTGAAATCATCTGGAAGTTTGTTTAGTAGTTTTAGATCGCCAAAATCTCAGGACGTCGCAATTTCATAACCTATCAACTTTGCATCATATTTTGAAATTAGACATGAATGAATACCATATTTAGTTGATCAACTTAGGGACAGTCCTGCGGTGGATTATGATATTTCAAACATTATGTTGATATTAGGAATGGGGTGATCGCTCAATGCTTGCAACCATCGCTCTTGGAGCTGCGCAGGTTCGATTTTATGTATTTATAAGCCTGCAATTCAGTCTCTGTTTTTCCCTGTTTTCATTTCAATCATATATTTCTCTTTCAATTTCCACTCTACAGTCTCCATGGGGTGTGGCCACGGGAGCTATTACTGGACACCTAATTGCTACCACCATTGCCATTCTTGGAGGAGCATTTCTTGCCAATTACATTTCTGAAAAACTGGTAGGAGCCTAACTTCTCTGTCATATAATGTGCGATTTGCTTTCTTAATCATTGATGCAACAACTCACTCTACCTTCAATCAGGTCGGCTACTTGGGCGGTGCACTCTTCTTAATTTTCGCAGTAGCAACTTTTTTTGGAGTTTTCTGA

mRNA sequence

ATGGAAGGATTGGTTGTTCGGTCGCCGTTCGTTTCAACGGGGAAGAAGCTTCCCTTCTCGCCTTTGCTCGATTCTTTGCCAAGTTCTTCTGCTTCTGGCAGTGTATTCGCGAAGGCCGTATCAACATCGCTGAAATGTAGAAGAGTGACGAGATTGAACGGGACGTATGGTAAAATCAGAGCACATTCGTCGAATGTAAGTATTGGATCCGATGGATACAGGCACGAGGAGGAAAAGGAGGGCCAGCATGTTATTTCCAGTAGTGCACCTGTTGATAGTAGTTCATCTAAAATTGAGAAGTCTCCAAATGGATTGCCTTATCCTCTTTCTATTGCATTAGTACTAATTGGGTGCAGTTTAGTATTCTCGTTGATTGCCTTTGTGAAAGGAGGACCTTCATCAATCTTGGCAGCAGTTGCGAAGTCAGGGTTCACTGCTGCCTTCTCATTAATATTTGTCTCTGAAATTGGGGACAAGACGTTTTTCATTGCTGCACTCTTGGCCATGCAATATAAGAAAGGACTGGTTCTGTTAGGATCTATGGGAGCACTTTCACTTATGACAGTCCTCTCAGTTATAATTGGACGGATATTTCACTCAGTACCTGCTCAATTCCAGACAACTTTACCAATTGGAGAATATGCGGCAGTAACACTTCTGTTGTTCTTTGGTCTCAAGTCCATTAAAGATGCATGGGATCTTCCCTCGAGTGTTCCTAAACAAGGTGATGAGAGTGGCCCAGAACTAGATGAATATGTCGAAGCGGAGGAGCTTGTTAAAGAAAAGGTTTCAAAACGGCTCTCAAATCCACTTGAAATCATCTGGAAGTCGTTCAGCCTTATATTTTTTGCTGAATGGGGTGATCGCTCAATGCTTGCAACCATCGCTCTTGGAGCTGCGCAGTCTCCATGGGGTGTGGCCACGGGAGCTATTACTGGACACCTAATTGCTACCACCATTGCCATTCTTGGAGGAGCATTTCTTGCCAATTACATTTCTGAAAAACTGGTCGGCTACTTGGGCGGTGCACTCTTCTTAATTTTCGCAGTAGCAACTTTTTTTGGAGTTTTCTGA

Coding sequence (CDS)

ATGGAAGGATTGGTTGTTCGGTCGCCGTTCGTTTCAACGGGGAAGAAGCTTCCCTTCTCGCCTTTGCTCGATTCTTTGCCAAGTTCTTCTGCTTCTGGCAGTGTATTCGCGAAGGCCGTATCAACATCGCTGAAATGTAGAAGAGTGACGAGATTGAACGGGACGTATGGTAAAATCAGAGCACATTCGTCGAATGTAAGTATTGGATCCGATGGATACAGGCACGAGGAGGAAAAGGAGGGCCAGCATGTTATTTCCAGTAGTGCACCTGTTGATAGTAGTTCATCTAAAATTGAGAAGTCTCCAAATGGATTGCCTTATCCTCTTTCTATTGCATTAGTACTAATTGGGTGCAGTTTAGTATTCTCGTTGATTGCCTTTGTGAAAGGAGGACCTTCATCAATCTTGGCAGCAGTTGCGAAGTCAGGGTTCACTGCTGCCTTCTCATTAATATTTGTCTCTGAAATTGGGGACAAGACGTTTTTCATTGCTGCACTCTTGGCCATGCAATATAAGAAAGGACTGGTTCTGTTAGGATCTATGGGAGCACTTTCACTTATGACAGTCCTCTCAGTTATAATTGGACGGATATTTCACTCAGTACCTGCTCAATTCCAGACAACTTTACCAATTGGAGAATATGCGGCAGTAACACTTCTGTTGTTCTTTGGTCTCAAGTCCATTAAAGATGCATGGGATCTTCCCTCGAGTGTTCCTAAACAAGGTGATGAGAGTGGCCCAGAACTAGATGAATATGTCGAAGCGGAGGAGCTTGTTAAAGAAAAGGTTTCAAAACGGCTCTCAAATCCACTTGAAATCATCTGGAAGTCGTTCAGCCTTATATTTTTTGCTGAATGGGGTGATCGCTCAATGCTTGCAACCATCGCTCTTGGAGCTGCGCAGTCTCCATGGGGTGTGGCCACGGGAGCTATTACTGGACACCTAATTGCTACCACCATTGCCATTCTTGGAGGAGCATTTCTTGCCAATTACATTTCTGAAAAACTGGTCGGCTACTTGGGCGGTGCACTCTTCTTAATTTTCGCAGTAGCAACTTTTTTTGGAGTTTTCTGA

Protein sequence

MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIRAHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSLVFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF
Homology
BLAST of Lcy10g014600 vs. ExPASy Swiss-Prot
Match: Q9T0H9 (Protein PAM71-homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM71-HL PE=1 SV=2)

HSP 1 Score: 436.0 bits (1120), Expect = 4.0e-121
Identity = 242/347 (69.74%), Postives = 277/347 (79.83%), Query Frame = 0

Query: 11  VSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIRAHSSNVSIGS 70
           VS+ +KLPF  L ++LP   +S     K     L+CRR  +L+  +GK R  +S+  +GS
Sbjct: 19  VSSIQKLPFLSLSETLPCPKSS----RKPTFLPLRCRRRPKLDLLWGKFRVRASDAGVGS 78

Query: 71  DGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSLVFSLIAFVKG 130
             Y   EE   Q      +P  SS S   + P   PY LSIALVL+ C LVFSLI FVKG
Sbjct: 79  GSYSGGEEDGSQSSSLDQSPATSSESLKPRGP--FPYSLSIALVLLSCGLVFSLITFVKG 138

Query: 131 GPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVL 190
           GPSS+LAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQY+K LVLLGSMGALSLMT+L
Sbjct: 139 GPSSVLAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYEKTLVLLGSMGALSLMTIL 198

Query: 191 SVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELD 250
           SV+IG+IF SVPAQFQTTLPIGEYAA+ LL+FFGLKSIKDAWDLP    K G+E+G EL 
Sbjct: 199 SVVIGKIFQSVPAQFQTTLPIGEYAAIALLMFFGLKSIKDAWDLPPVEAKNGEETGIELG 258

Query: 251 EYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGA 310
           EY EAEELVKEK SK+L+NPLEI+WKSFSL+FFAEWGDRSMLAT+ALGAAQSP GVA+GA
Sbjct: 259 EYSEAEELVKEKASKKLTNPLEILWKSFSLVFFAEWGDRSMLATVALGAAQSPLGVASGA 318

Query: 311 ITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           I GHL+AT +AI+GGAFLANYISEKLVGY+GGALFL+FA ATFFGVF
Sbjct: 319 IAGHLVATVLAIMGGAFLANYISEKLVGYVGGALFLVFAAATFFGVF 359

BLAST of Lcy10g014600 vs. ExPASy Swiss-Prot
Match: Q2R2Z4 (GDT1-like protein 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0544500 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.8e-100
Identity = 208/320 (65.00%), Postives = 251/320 (78.44%), Query Frame = 0

Query: 40  VSTSLKCRRVTRLNGTYGKIRAHSSNVSIGSDGYR-HEEEKEGQHVISSSAPVDSSSSKI 99
           V   ++C R+  L      +R  +SNV++G+  Y   E    G+H+ SS+      S+K 
Sbjct: 34  VEGQVRC-RLPWLRPIRHNVRVQTSNVNVGAGSYEGGEAGSHGEHLDSSAT---RDSNKP 93

Query: 100 EKSPNGLPYPLSIALVLIGCSLVFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGD 159
            K P+G  YP SIA VL+ C+L  + I F KG PS+++A +AKSGFTAAF+LIFVSEIGD
Sbjct: 94  TKPPSGSRYPQSIAAVLLLCALASAFIVFFKGQPSAVVAMLAKSGFTAAFTLIFVSEIGD 153

Query: 160 KTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVT 219
           KTFFIAALLAMQY++ LVLLGSM ALSLMT++SVIIGRIF SVPAQFQTTLPIGEYAA+ 
Sbjct: 154 KTFFIAALLAMQYQRALVLLGSMAALSLMTIVSVIIGRIFQSVPAQFQTTLPIGEYAAIA 213

Query: 220 LLLFFGLKSIKDAWDLPSSVPKQGDESG-PELDEYVEAEELVKEKVSKRLSNPLEIIWKS 279
           LL FFG KSIKDAW LP +    G+  G  E  E  EAEELVKEKV+K+L++PLE++WKS
Sbjct: 214 LLAFFGFKSIKDAWQLPDNA--NGNLQGNSESGELAEAEELVKEKVAKKLTSPLEVLWKS 273

Query: 280 FSLIFFAEWGDRSMLATIALGAAQSPWGVATGAITGHLIATTIAILGGAFLANYISEKLV 339
           FSL+FFAEWGDRSMLATIALGAAQSP+GVA+GAI GHL+AT +AI+GGAFLANY+SEKLV
Sbjct: 274 FSLVFFAEWGDRSMLATIALGAAQSPFGVASGAIAGHLVATFLAIVGGAFLANYLSEKLV 333

Query: 340 GYLGGALFLIFAVATFFGVF 358
           G +GG LFL+FAVATFFGVF
Sbjct: 334 GLIGGVLFLLFAVATFFGVF 347

BLAST of Lcy10g014600 vs. ExPASy Swiss-Prot
Match: Q94AX5 (Protein PAM71, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM71 PE=1 SV=2)

HSP 1 Score: 166.4 bits (420), Expect = 5.9e-40
Identity = 102/218 (46.79%), Postives = 136/218 (62.39%), Query Frame = 0

Query: 142 SGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSVIIGRIFHSV 201
           SGF +AF LIF SE+GDKTFFIAALLA +     V +G+ GAL +MT++SV++GR FH V
Sbjct: 157 SGFASAFLLIFFSELGDKTFFIAALLAARNSAATVFVGTFGALGIMTIISVVLGRTFHYV 216

Query: 202 ----PAQF-QTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELDEYVEAE 261
               P +F  T LPI + AAV LL++FG+ ++ DA            + G   +E  EAE
Sbjct: 217 DEVLPFRFGGTDLPIDDIAAVCLLVYFGVSTLLDA----------VSDEGKADEEQKEAE 276

Query: 262 ELVKEKVSK--RLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGAITGH 321
             V E       +      I  +F+L+F AEWGD+S  +TIAL AA SP GV  GA+ GH
Sbjct: 277 LAVSELSGNGAGIVAAANTIISTFALVFVAEWGDKSFFSTIALAAASSPLGVIAGALAGH 336

Query: 322 LIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVAT 353
             AT +A+LGG+ L N++SEK + Y+GG LFL+FA  T
Sbjct: 337 GAATLLAVLGGSLLGNFLSEKAIAYVGGVLFLVFAAVT 364

BLAST of Lcy10g014600 vs. ExPASy Swiss-Prot
Match: Q5NAY7 (GDT1-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0221700 PE=3 SV=2)

HSP 1 Score: 165.2 bits (417), Expect = 1.3e-39
Identity = 101/222 (45.50%), Postives = 141/222 (63.51%), Query Frame = 0

Query: 142 SGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSVIIGRIFHSV 201
           +GF +AF LIF SE+GD+TFFIAALLA +    ++ LG+ GAL++MT++SV++GR FH V
Sbjct: 126 TGFASAFLLIFFSELGDRTFFIAALLAARNSGAIIFLGTFGALAVMTIISVVLGRAFHYV 185

Query: 202 ----PAQF-QTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELDEYVEAE 261
               P  F  T  P+ ++ A  LL+++G+ ++ DA          GDE   +++E  E  
Sbjct: 186 DGIIPFSFGGTDFPVDDFLAACLLVYYGITTLLDA--------ASGDEE--KMNEEQEEA 245

Query: 262 ELVKEKVSKRLSNPLEI------IWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGA 321
           EL    VSK L N   I      I  +F L+F AEWGD+S  +TIAL AA SP GV  G+
Sbjct: 246 ELA---VSKFLGNGAGIISAASTIASTFVLVFIAEWGDKSFFSTIALAAASSPLGVIAGS 305

Query: 322 ITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVAT 353
           + GH +AT IA+LGG+ L  ++SEK+V Y+GG+LFL FA  T
Sbjct: 306 LAGHAVATLIAVLGGSLLGTFLSEKIVAYIGGSLFLAFAAVT 334

BLAST of Lcy10g014600 vs. ExPASy Swiss-Prot
Match: B8AAM2 (GDT1-like protein 1, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_00941 PE=3 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 1.7e-39
Identity = 101/222 (45.50%), Postives = 141/222 (63.51%), Query Frame = 0

Query: 142 SGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSVIIGRIFHSV 201
           +GF +AF LIF SE+GD+TFFIAALLA +    ++ LG+ GAL++MT++SV++GR FH V
Sbjct: 126 TGFASAFLLIFFSELGDRTFFIAALLAARNSGAIIFLGTFGALAVMTIISVVLGRAFHYV 185

Query: 202 ----PAQF-QTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELDEYVEAE 261
               P  F  T  P+ ++ A  LL+++G+ ++ DA          GDE   +++E  E  
Sbjct: 186 DGIIPFSFGGTDFPVDDFLAACLLVYYGVTTLLDA--------ASGDEE--KMNEEQEEA 245

Query: 262 ELVKEKVSKRLSNPLEI------IWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGA 321
           EL    VSK L N   I      I  +F L+F AEWGD+S  +TIAL AA SP GV  G+
Sbjct: 246 ELA---VSKFLGNGAGIISAASTIASTFVLVFIAEWGDKSFFSTIALAAASSPLGVIAGS 305

Query: 322 ITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVAT 353
           + GH +AT IA+LGG+ L  ++SEK+V Y+GG+LFL FA  T
Sbjct: 306 LAGHAVATLIAVLGGSLLGTFLSEKIVAYIGGSLFLAFAAVT 334

BLAST of Lcy10g014600 vs. ExPASy TrEMBL
Match: A0A6J1IBK7 (GDT1 family protein OS=Cucurbita maxima OX=3661 GN=LOC111473197 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.5e-176
Identity = 340/357 (95.24%), Postives = 344/357 (96.36%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKK PFSPLLDSLPS SASGSVFAK VS SLKCRRVTRLN  YGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKFPFSPLLDSLPSCSASGSVFAKTVSKSLKCRRVTRLNTKYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKEGQHVISSSAP D SSSK EKSPNGLPYPLSIALVLIGCSL
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEGQHVISSSAPAD-SSSKTEKSPNGLPYPLSIALVLIGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAW+LPSSVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWELPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           Q +ES PELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QDNESPPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGY+GG LFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYVGGVLFLIFAVATFFGVF 356

BLAST of Lcy10g014600 vs. ExPASy TrEMBL
Match: A0A6J1G9C7 (GDT1 family protein OS=Cucurbita moschata OX=3662 GN=LOC111452154 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.5e-176
Identity = 340/357 (95.24%), Postives = 344/357 (96.36%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKKLPFSPLLDSLPS SASGSVFAK VS SLKCRRVTRLN  YGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSCSASGSVFAKTVSKSLKCRRVTRLNTKYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSN SIGSDGY+HEEEKEGQHVISSSAP D SSSK EKSPNGLPYPLSIALVLIGCSL
Sbjct: 61  AHSSNASIGSDGYKHEEEKEGQHVISSSAPAD-SSSKTEKSPNGLPYPLSIALVLIGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAW+LPSSVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWELPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           Q +ES PELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QDNESPPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGY+GG LFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYVGGVLFLIFAVATFFGVF 356

BLAST of Lcy10g014600 vs. ExPASy TrEMBL
Match: A0A6J1IBI9 (GDT1 family protein OS=Cucurbita maxima OX=3661 GN=LOC111473663 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.7e-176
Identity = 340/357 (95.24%), Postives = 344/357 (96.36%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKK PFSPLLDSLPS SASGSVFAK VS SLKCRRVTRLN  YGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKFPFSPLLDSLPSCSASGSVFAKNVSKSLKCRRVTRLNTKYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKEGQHVISSSAP D SSSK EKSPNGLPYPLSIALVLIGCSL
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEGQHVISSSAPAD-SSSKTEKSPNGLPYPLSIALVLIGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAW+LPSSVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWELPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           Q +ES PELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QDNESPPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGY+GG LFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYVGGVLFLIFAVATFFGVF 356

BLAST of Lcy10g014600 vs. ExPASy TrEMBL
Match: A0A6J1DZQ1 (GDT1 family protein OS=Momordica charantia OX=3673 GN=LOC111024580 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 332/357 (93.00%), Postives = 344/357 (96.36%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           M+GLVV+SPFVS GKKLPFSPLLDSLPS SASGSV  K VSTS +CRRVTRL+GT+G++R
Sbjct: 1   MQGLVVQSPFVSRGKKLPFSPLLDSLPSCSASGSVPGKTVSTSQRCRRVTRLSGTFGELR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKE QHVISSSAP DSSSSK EKSPNGLPYPLSIALVL GCSL
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEDQHVISSSAPADSSSSKTEKSPNGLPYPLSIALVLFGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLL FFG+KSIKDAWDLP+SVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLFFFGIKSIKDAWDLPASVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
            G+ESGPELDEY EAEELVKEKVSK+LSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 HGNESGPELDEYAEAEELVKEKVSKQLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 357

BLAST of Lcy10g014600 vs. ExPASy TrEMBL
Match: A0A1S3AWY0 (GDT1 family protein OS=Cucumis melo OX=3656 GN=LOC103483513 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.6e-173
Identity = 335/357 (93.84%), Postives = 342/357 (95.80%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKKLPFSPLLDSLPS SASGSVF+K VSTSLK  RVT L+ TYGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSCSASGSVFSKTVSTSLKRTRVTSLSRTYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKEG HVIS SAP D SSSK EKSP+GLPYPLSIALVLIGC L
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEGHHVISGSAP-DISSSKTEKSPSGLPYPLSIALVLIGCGL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQY+KGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYEKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMT LSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLK+IKDAWDLPSSVPK
Sbjct: 181 MGALSLMTALSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKAIKDAWDLPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGYLGG LFLIFA+ATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYLGGVLFLIFAIATFFGVF 356

BLAST of Lcy10g014600 vs. NCBI nr
Match: XP_038900282.1 (protein PAM71-homolog, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 639.0 bits (1647), Expect = 2.4e-179
Identity = 341/357 (95.52%), Postives = 346/357 (96.92%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKKLPFSPLLDSLP  SASG +FAK V TSLK RRVTRLNGTYGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPCRSASGGIFAKTVPTSLKRRRVTRLNGTYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKEGQHV+SSSAPVD SSSK EKSP GLPYPLSIALVLIGC L
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEGQHVVSSSAPVDISSSKTEKSPKGLPYPLSIALVLIGCGL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSS+PK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSIPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGG LFLIFA+ATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGVLFLIFAIATFFGVF 357

BLAST of Lcy10g014600 vs. NCBI nr
Match: XP_023521675.1 (protein PAM71-homolog, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023539860.1 protein PAM71-homolog, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.5 bits (1617), Expect = 7.2e-176
Identity = 341/357 (95.52%), Postives = 345/357 (96.64%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKKLPFSPLLDSLPS SASGSVFAK VS SLKCRRVTRLN  YGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSCSASGSVFAKTVSKSLKCRRVTRLNTKYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKEGQHVISSSAP D SSSK EKSPNGLPYPLSIALVLIGCSL
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEGQHVISSSAPGD-SSSKTEKSPNGLPYPLSIALVLIGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAW+LPSSVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWELPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           Q +ES PELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QDNESPPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGY+GG LFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYVGGVLFLIFAVATFFGVF 356

BLAST of Lcy10g014600 vs. NCBI nr
Match: XP_022948496.1 (protein PAM71-homolog, chloroplastic [Cucurbita moschata] >KAG6597316.1 Protein PAM71-homolog, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 627.1 bits (1616), Expect = 9.4e-176
Identity = 340/357 (95.24%), Postives = 344/357 (96.36%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKKLPFSPLLDSLPS SASGSVFAK VS SLKCRRVTRLN  YGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSCSASGSVFAKTVSKSLKCRRVTRLNTKYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSN SIGSDGY+HEEEKEGQHVISSSAP D SSSK EKSPNGLPYPLSIALVLIGCSL
Sbjct: 61  AHSSNASIGSDGYKHEEEKEGQHVISSSAPAD-SSSKTEKSPNGLPYPLSIALVLIGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAW+LPSSVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWELPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           Q +ES PELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QDNESPPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGY+GG LFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYVGGVLFLIFAVATFFGVF 356

BLAST of Lcy10g014600 vs. NCBI nr
Match: XP_022974520.1 (protein PAM71-homolog, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022974521.1 protein PAM71-homolog, chloroplastic-like isoform X2 [Cucurbita maxima] >XP_022974522.1 protein PAM71-homolog, chloroplastic-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 627.1 bits (1616), Expect = 9.4e-176
Identity = 340/357 (95.24%), Postives = 344/357 (96.36%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKK PFSPLLDSLPS SASGSVFAK VS SLKCRRVTRLN  YGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKFPFSPLLDSLPSCSASGSVFAKTVSKSLKCRRVTRLNTKYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKEGQHVISSSAP D SSSK EKSPNGLPYPLSIALVLIGCSL
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEGQHVISSSAPAD-SSSKTEKSPNGLPYPLSIALVLIGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAW+LPSSVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWELPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           Q +ES PELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QDNESPPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGY+GG LFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYVGGVLFLIFAVATFFGVF 356

BLAST of Lcy10g014600 vs. NCBI nr
Match: XP_022974902.1 (protein PAM71-homolog, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 626.3 bits (1614), Expect = 1.6e-175
Identity = 340/357 (95.24%), Postives = 344/357 (96.36%), Query Frame = 0

Query: 1   MEGLVVRSPFVSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIR 60
           MEGLVVRSPFVSTGKK PFSPLLDSLPS SASGSVFAK VS SLKCRRVTRLN  YGKIR
Sbjct: 1   MEGLVVRSPFVSTGKKFPFSPLLDSLPSCSASGSVFAKNVSKSLKCRRVTRLNTKYGKIR 60

Query: 61  AHSSNVSIGSDGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSL 120
           AHSSNVSIGSDGY+HEEEKEGQHVISSSAP D SSSK EKSPNGLPYPLSIALVLIGCSL
Sbjct: 61  AHSSNVSIGSDGYKHEEEKEGQHVISSSAPAD-SSSKTEKSPNGLPYPLSIALVLIGCSL 120

Query: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180
           VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS
Sbjct: 121 VFSLIAFVKGGPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGS 180

Query: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPK 240
           MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAW+LPSSVPK
Sbjct: 181 MGALSLMTVLSVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWELPSSVPK 240

Query: 241 QGDESGPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300
           Q +ES PELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA
Sbjct: 241 QDNESPPELDEYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAA 300

Query: 301 QSPWGVATGAITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           QSPWGVATGAITGHLIATTIAILGGA LANYISEKLVGY+GG LFLIFAVATFFGVF
Sbjct: 301 QSPWGVATGAITGHLIATTIAILGGALLANYISEKLVGYVGGVLFLIFAVATFFGVF 356

BLAST of Lcy10g014600 vs. TAIR 10
Match: AT4G13590.1 (Uncharacterized protein family (UPF0016) )

HSP 1 Score: 436.0 bits (1120), Expect = 2.9e-122
Identity = 242/347 (69.74%), Postives = 277/347 (79.83%), Query Frame = 0

Query: 11  VSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIRAHSSNVSIGS 70
           VS+ +KLPF  L ++LP   +S     K     L+CRR  +L+  +GK R  +S+  +GS
Sbjct: 19  VSSIQKLPFLSLSETLPCPKSS----RKPTFLPLRCRRRPKLDLLWGKFRVRASDAGVGS 78

Query: 71  DGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSLVFSLIAFVKG 130
             Y   EE   Q      +P  SS S   + P   PY LSIALVL+ C LVFSLI FVKG
Sbjct: 79  GSYSGGEEDGSQSSSLDQSPATSSESLKPRGP--FPYSLSIALVLLSCGLVFSLITFVKG 138

Query: 131 GPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVL 190
           GPSS+LAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQY+K LVLLGSMGALSLMT+L
Sbjct: 139 GPSSVLAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYEKTLVLLGSMGALSLMTIL 198

Query: 191 SVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELD 250
           SV+IG+IF SVPAQFQTTLPIGEYAA+ LL+FFGLKSIKDAWDLP    K G+E+G EL 
Sbjct: 199 SVVIGKIFQSVPAQFQTTLPIGEYAAIALLMFFGLKSIKDAWDLPPVEAKNGEETGIELG 258

Query: 251 EYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGA 310
           EY EAEELVKEK SK+L+NPLEI+WKSFSL+FFAEWGDRSMLAT+ALGAAQSP GVA+GA
Sbjct: 259 EYSEAEELVKEKASKKLTNPLEILWKSFSLVFFAEWGDRSMLATVALGAAQSPLGVASGA 318

Query: 311 ITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           I GHL+AT +AI+GGAFLANYISEKLVGY+GGALFL+FA ATFFGVF
Sbjct: 319 IAGHLVATVLAIMGGAFLANYISEKLVGYVGGALFLVFAAATFFGVF 359

BLAST of Lcy10g014600 vs. TAIR 10
Match: AT4G13590.2 (Uncharacterized protein family (UPF0016) )

HSP 1 Score: 435.3 bits (1118), Expect = 4.9e-122
Identity = 242/347 (69.74%), Postives = 276/347 (79.54%), Query Frame = 0

Query: 11  VSTGKKLPFSPLLDSLPSSSASGSVFAKAVSTSLKCRRVTRLNGTYGKIRAHSSNVSIGS 70
           VS+ +KLPF  L ++LP   +S     K      KCRR  +L+  +GK R  +S+  +GS
Sbjct: 19  VSSIQKLPFLSLSETLPCPKSS----RKPTFLPCKCRRRPKLDLLWGKFRVRASDAGVGS 78

Query: 71  DGYRHEEEKEGQHVISSSAPVDSSSSKIEKSPNGLPYPLSIALVLIGCSLVFSLIAFVKG 130
             Y   EE   Q      +P  SS S   + P   PY LSIALVL+ C LVFSLI FVKG
Sbjct: 79  GSYSGGEEDGSQSSSLDQSPATSSESLKPRGP--FPYSLSIALVLLSCGLVFSLITFVKG 138

Query: 131 GPSSILAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVL 190
           GPSS+LAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQY+K LVLLGSMGALSLMT+L
Sbjct: 139 GPSSVLAAVAKSGFTAAFSLIFVSEIGDKTFFIAALLAMQYEKTLVLLGSMGALSLMTIL 198

Query: 191 SVIIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELD 250
           SV+IG+IF SVPAQFQTTLPIGEYAA+ LL+FFGLKSIKDAWDLP    K G+E+G EL 
Sbjct: 199 SVVIGKIFQSVPAQFQTTLPIGEYAAIALLMFFGLKSIKDAWDLPPVEAKNGEETGIELG 258

Query: 251 EYVEAEELVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGA 310
           EY EAEELVKEK SK+L+NPLEI+WKSFSL+FFAEWGDRSMLAT+ALGAAQSP GVA+GA
Sbjct: 259 EYSEAEELVKEKASKKLTNPLEILWKSFSLVFFAEWGDRSMLATVALGAAQSPLGVASGA 318

Query: 311 ITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFFGVF 358
           I GHL+AT +AI+GGAFLANYISEKLVGY+GGALFL+FA ATFFGVF
Sbjct: 319 IAGHLVATVLAIMGGAFLANYISEKLVGYVGGALFLVFAAATFFGVF 359

BLAST of Lcy10g014600 vs. TAIR 10
Match: AT1G64150.1 (Uncharacterized protein family (UPF0016) )

HSP 1 Score: 166.4 bits (420), Expect = 4.2e-41
Identity = 102/218 (46.79%), Postives = 136/218 (62.39%), Query Frame = 0

Query: 142 SGFTAAFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSVIIGRIFHSV 201
           SGF +AF LIF SE+GDKTFFIAALLA +     V +G+ GAL +MT++SV++GR FH V
Sbjct: 157 SGFASAFLLIFFSELGDKTFFIAALLAARNSAATVFVGTFGALGIMTIISVVLGRTFHYV 216

Query: 202 ----PAQF-QTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELDEYVEAE 261
               P +F  T LPI + AAV LL++FG+ ++ DA            + G   +E  EAE
Sbjct: 217 DEVLPFRFGGTDLPIDDIAAVCLLVYFGVSTLLDA----------VSDEGKADEEQKEAE 276

Query: 262 ELVKEKVSK--RLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGAITGH 321
             V E       +      I  +F+L+F AEWGD+S  +TIAL AA SP GV  GA+ GH
Sbjct: 277 LAVSELSGNGAGIVAAANTIISTFALVFVAEWGDKSFFSTIALAAASSPLGVIAGALAGH 336

Query: 322 LIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVAT 353
             AT +A+LGG+ L N++SEK + Y+GG LFL+FA  T
Sbjct: 337 GAATLLAVLGGSLLGNFLSEKAIAYVGGVLFLVFAAVT 364

BLAST of Lcy10g014600 vs. TAIR 10
Match: AT5G36290.1 (Uncharacterized protein family (UPF0016) )

HSP 1 Score: 133.7 bits (335), Expect = 3.0e-31
Identity = 94/224 (41.96%), Postives = 137/224 (61.16%), Query Frame = 0

Query: 136 LAAVAKSGFTA---AFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSV 195
           L A A S F A   +FS+I V+EIGD+TF IAAL+AM++ K  VL G++ AL +MT+LS 
Sbjct: 74  LDATAPSVFDALFSSFSMILVTEIGDETFIIAALMAMRHPKATVLSGALSALFVMTILST 133

Query: 196 IIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELDEY 255
            +GRI  ++ ++  T       AA  L  FFGL+ +  AW    S   Q  E   E++E 
Sbjct: 134 GLGRIVPNLISRKHT-----NSAATVLYAFFGLRLLYIAWRSTDSKSNQKKEM-EEVEEK 193

Query: 256 VEAEE--LVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGA 315
           +E+ +      ++  R   P  I  +SF L F AEWGDRS +ATIAL   ++  GVA GA
Sbjct: 194 LESGQGKTPFRRLFSRFCTP--IFLESFILTFLAEWGDRSQIATIALATHKNAIGVAIGA 253

Query: 316 ITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFF 355
             GH + T++A++GG+ LA+ IS++ V  +GG LFL F+V+++F
Sbjct: 254 SIGHTVCTSLAVVGGSMLASRISQRTVATVGGLLFLGFSVSSYF 289

BLAST of Lcy10g014600 vs. TAIR 10
Match: AT5G36290.2 (Uncharacterized protein family (UPF0016) )

HSP 1 Score: 133.7 bits (335), Expect = 3.0e-31
Identity = 94/224 (41.96%), Postives = 137/224 (61.16%), Query Frame = 0

Query: 136 LAAVAKSGFTA---AFSLIFVSEIGDKTFFIAALLAMQYKKGLVLLGSMGALSLMTVLSV 195
           L A A S F A   +FS+I V+EIGD+TF IAAL+AM++ K  VL G++ AL +MT+LS 
Sbjct: 74  LDATAPSVFDALFSSFSMILVTEIGDETFIIAALMAMRHPKATVLSGALSALFVMTILST 133

Query: 196 IIGRIFHSVPAQFQTTLPIGEYAAVTLLLFFGLKSIKDAWDLPSSVPKQGDESGPELDEY 255
            +GRI  ++ ++  T       AA  L  FFGL+ +  AW    S   Q  E   E++E 
Sbjct: 134 GLGRIVPNLISRKHT-----NSAATVLYAFFGLRLLYIAWRSTDSKSNQKKEM-EEVEEK 193

Query: 256 VEAEE--LVKEKVSKRLSNPLEIIWKSFSLIFFAEWGDRSMLATIALGAAQSPWGVATGA 315
           +E+ +      ++  R   P  I  +SF L F AEWGDRS +ATIAL   ++  GVA GA
Sbjct: 194 LESGQGKTPFRRLFSRFCTP--IFLESFILTFLAEWGDRSQIATIALATHKNAIGVAIGA 253

Query: 316 ITGHLIATTIAILGGAFLANYISEKLVGYLGGALFLIFAVATFF 355
             GH + T++A++GG+ LA+ IS++ V  +GG LFL F+V+++F
Sbjct: 254 SIGHTVCTSLAVVGGSMLASRISQRTVATVGGLLFLGFSVSSYF 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0H94.0e-12169.74Protein PAM71-homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM71-HL... [more]
Q2R2Z41.8e-10065.00GDT1-like protein 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q94AX55.9e-4046.79Protein PAM71, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM71 PE=1 SV=2[more]
Q5NAY71.3e-3945.50GDT1-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
B8AAM21.7e-3945.50GDT1-like protein 1, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI... [more]
Match NameE-valueIdentityDescription
A0A6J1IBK74.5e-17695.24GDT1 family protein OS=Cucurbita maxima OX=3661 GN=LOC111473197 PE=3 SV=1[more]
A0A6J1G9C74.5e-17695.24GDT1 family protein OS=Cucurbita moschata OX=3662 GN=LOC111452154 PE=3 SV=1[more]
A0A6J1IBI97.7e-17695.24GDT1 family protein OS=Cucurbita maxima OX=3661 GN=LOC111473663 PE=3 SV=1[more]
A0A6J1DZQ11.1e-17493.00GDT1 family protein OS=Momordica charantia OX=3673 GN=LOC111024580 PE=3 SV=1[more]
A0A1S3AWY01.6e-17393.84GDT1 family protein OS=Cucumis melo OX=3656 GN=LOC103483513 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038900282.12.4e-17995.52protein PAM71-homolog, chloroplastic isoform X1 [Benincasa hispida][more]
XP_023521675.17.2e-17695.52protein PAM71-homolog, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_02353... [more]
XP_022948496.19.4e-17695.24protein PAM71-homolog, chloroplastic [Cucurbita moschata] >KAG6597316.1 Protein ... [more]
XP_022974520.19.4e-17695.24protein PAM71-homolog, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_0229... [more]
XP_022974902.11.6e-17595.24protein PAM71-homolog, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G13590.12.9e-12269.74Uncharacterized protein family (UPF0016) [more]
AT4G13590.24.9e-12269.74Uncharacterized protein family (UPF0016) [more]
AT1G64150.14.2e-4146.79Uncharacterized protein family (UPF0016) [more]
AT5G36290.13.0e-3141.96Uncharacterized protein family (UPF0016) [more]
AT5G36290.23.0e-3141.96Uncharacterized protein family (UPF0016) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001727Gdt1 familyPFAMPF01169UPF0016coord: 276..349
e-value: 1.5E-20
score: 73.3
coord: 146..224
e-value: 1.3E-19
score: 70.3
IPR001727Gdt1 familyPANTHERPTHR12608TRANSMEMBRANE PROTEIN HTP-1 RELATEDcoord: 38..357
IPR001727Gdt1 familyPROSITEPS01214UPF0016coord: 155..164
NoneNo IPR availablePANTHERPTHR12608:SF7PROTEIN PAM71-HOMOLOG, CHLOROPLASTICcoord: 38..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g014600.1Lcy10g014600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0032468 Golgi calcium ion homeostasis
biological_process GO:0032472 Golgi calcium ion transport
biological_process GO:0071421 manganese ion transmembrane transport
cellular_component GO:0009507 chloroplast
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015085 calcium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity