Lcy10g014150 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g014150
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionLong-chain-alcohol oxidase
LocationChr10: 42270915 .. 42276510 (+)
RNA-Seq ExpressionLcy10g014150
SyntenyLcy10g014150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGGCGGTACTGGTAGGGCTGAGCATGATGAGAGAATGGGAATCAAAAAACAGAAAGGTAATCAAATTTACCAAATAGCCCTTGAATTTCCAAACCATTCCTATATATAGAGAGCTCACGTCTTCCGGCAAAGGCATATCGAGTTCATCTGCAGAGAAACACGATGCGGAGATCGGAGTGTCATCCTCTGTTGAGAGGAACAAGGAGCGATAAGTTCAGCCATGGCTTCTCTGCGGCGGAGATGGACTCATTGGCCTCCATCTGCGACGCCGTTCTTCCTCCTCTGCCGTTCTATTCACCAAACAACGGCCGCGATGAAATCGATGCTTCCGAGGCCGCCGCCGTGAAGGCGTTTTACGCGGCGTCCGGTTCGCAATCTCCGATTCCAGACGAGGTGCGTTGATTGACGGATGGAGTTTTCTTCATCTTCTTCTTCTTTAATTTTGTGATGTGTTTTTGGAAATTAAAAATTAGGTTGCGGAGACGGTGACGAAGAGAGGATTGATTGAAATTGTGATAGTTATAAGGGCGGTGCTGTGGATTCTGGCTACGAGGTTAGGGACGTTGTTGCTCTGTGGATCGCTTTGTTTCTGCGAGAAATGGCCTTTCGTCTGCAATTTCTCGGCTATGCCATTGGAGAAGAGAGAGGAAGCTTTGAGGAGATGGTTTAAGAATAAGGTTTTCACGCCCATTAGAACTGCTCTTGTTGGCGTCAGAGTTCTTTCCCTCTATATCTTCTTTTCTCGGGTAATCCCTCTCTCTCTCCCTTAACTTTTGCCATTTTTCCATTTTTCCCTCTTAATTTTTAGAATGCTTAATTTTTAAAAAGTAAAGATAAAGATTGGAAGGGATATATGTTTTTTAATTTGTGTCCAAAAACACATTTTAAGAGTTAAGTTCAAGAAATCCTCTTAAAAACCCACAGAGCCACATGCTATGCTAATATATATTATTTTTATAAGTTATAAATTTATAAACCAATATTTAAAACTTTTTTTTTTATAAATAAATTCTCATTTTCAAGTTCATACCCTAAATTTTACATACCCAAATTTGTTGAAAGTAGTAACCAAATATCCCTTCAATAAATTCGAACTTTACAAGTTCTGTCAAAGTAAATAAATTAAAAGTAAAATTGTACCATCTAACACTGAATATTATATTATATTTACATCAAAGACCTCTGTAATTAATAATCAATTTTTGTTTCTGTTAATATTATTCAAATTTTTAGTCTACCTTTCACAAATATTTTGTAATCCTAAACAAATCTTAAAAATGAATTTAGTACAAAATTTCGAGTTGCTTCTTCTAATCAACTTTCACTTTTTTTAGCTCATTTGCTACTCCTATAATAGGAAAAAAAAAAAAAAAAAGAGAATATATTGATATAAGATTTTTATATAAGGGCTGAAATCATTTATATGTGTGAGTATTAGAAAGAGCAATAAAGATGGGACAGAGACGTTAACTGGATACCTACTTAAATAAAGTATGGATTTAATGCCAAACTTGTGAGTGTAGCTGCTGAATACATATAAAGTAAAGTTATTAAATGCAAACATAAAGAAGGAAGAAAGGTAAGTGAAAAGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATAAAAATAATGTAGGTTTTATTGACAATAATATAAAAAGTACTTTTATTCAATACGAAGTGTAAATTATTTATCCTAAACATATTTTTTCTTTATGATTATTTCCAGGAATTTTTTTTGGGGGAGGAAATTAATTGTCCAAAATTGTCTATCTTATAAAAGATGCATGATTTATCATATTTCAAAAAAAAAAAAGATGCAAGATTTATCATGTTTAGAAATTAATGACCAACATTTTCATTTTTTAAAATTGTTTTTGAAAAAAAAAACATTTAAAAATATTATCCTTTTTCTATAAAAGATTCACAAGATCCCAAAGAAACGAAGAGATTTGTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAATCGAGAGATCATAAAGTCAAAAAAAGGAAAATATAAAAGTCAAACATGATACCATTTAGAAGGGTAGGGCCCTTGGCCTGCAGCAAAATCGCAGCCAGAGCACGTGCTGTTTGATTTGTCTGTTTTGTAATTTGGCAAGGACAAACTAAAAGAGGAAAGAAAAAAGGCATACAAAATAGAAAACAAATATATTTTGATATTTTATTTTAAAAAAAAACTAAAGTTATTGTTCATGTTTTATGATTAAACGTTAAACTAATAAAAATGTAGACATTTTGACTTGGGGTACAATTTGAGATTATTCTTGGACTGGTACAATTTTTTTTTAGCACACCAAAAGTATTTAAAAATTTTCCCAATGTTTGGTTATATAATGAAAAGTAATTTTAAAAATATTTAAAACATTAGTAATTTTATGAGAAATATTTAATTAGTGTGCAATTGGGCATGGAAGAAGAAAATTTCTAGTACCACAACAATACATACTTTCTTTGTTCACATTGCAACTTTGTCTTCGTAAATATATTTTTAATGTTGGTGGACCTCACATCATTTTTCAAATGATGATGATATGACAAGTAATAAATGGATAGAGCATCTTGCATGAAAGAAATACTTTTTATTTGTTTGAGTATTTCTTGTTCTAAATTTTTTTCATACAATAAAGTTGAAGTGAAATTAATTGTGTGTATATATATAAAAAAAAAGGAGATATTAAACGATCTAACCACTCCAACTATACTCAATAAATCAAATCAACCATTTAAATCATGTTTGCAATTTGCATGAACAAGTAAGACCACAGTTTTTTCTTTTTCTTTTTTTTTTTGGGAAACCAGTAAGTCCACAATTTTATTTATTTATTTTTTAAAAAGAAAAGTGATCCATAAATGTGTAACCAGCAAATGACGAGAATGGCCTTTTTTGGATTTACTGTAATATGAATATAGGATATGAAAATAAAAATTCATATGTTATTTTTTTTTTATTTCTTTTTTGAGTTCTAATGTCATATTTTATTTATTGTTTTTTTAAAATAATATTTTATTTATTGTTAACAAAAAAGTGTTTGGTTTTCAAATTTGTGGTCTATTATAATTCAATTTCTTTTCTGTTATAAGCCACGTCCTTTTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATTTAATACATTTCCCTTTCCATTCATCAAATTCAATAGGTTTTTTTTTTTTTCACTTAAAATATAGGTCATTTACTTCATTCAATCACTTTTTATCTTCCCTTTCGTATTCATTTATTGATTCATTTAGTCTTCATTTATTCATTTTTCAAAAATTAAAGAAATTCATTTTTCATGTGAAGTATCATATATACAATCAAGAAGTCAAAAGTTCAAATCTTTCTACCACCACGTATTGTTAGGTTCATGACAAACATCTTATCCACTAAACTATGTTCAAATTGACAAAAAAAAAAAAAAAAAAAAAAAAATCCTCCTTTGACAACATTCAATTTTTGACAAATAGTTTTTTTTTTCCTACTCTAAATTATATCAATTTAACTCATGCAATGATTCAAAAAGGATGAGAAAGATTTTACTTCATCTCAGTTTCAAAAAAAAAAAAAAAATTATTTATCGTGTTTGAATGTATATGCCTTGACTATATTCAAATTAGTTGTCATAGTGTTTTCTCATTACAAATTTTTGTGTAAAATTTATAATTAAAAATACAATCTTTTAAAGTAGAGAGACAAGAACAAAGAAAGAGTTGAAGTTCAAGAGTGAAATTAGTAAACAAAAAAAGAATCCTTATTTTCAGGGGAAATGGCATCATCATTGAATAATTTTTCGTTGCTTTGACACAGGTTGATGTAAATGGCGATAACTCAAGTTGGGAAGCCATAAAATACCGGCCGGCCATTGATGAAAATCCGTCCAAATCCTCCGACGAGAGGCCTCTCCAGAAGGGAATCATAGAACCCAACAACGTAACCGAATCATCCTTTCGAAAATCCCTCTTACAGAAAGGTCTCAAAGTCACCGGAGACGACGGCCACACCCTTTCCATCGAATGCGATGTGGTGGTTGTTGGGTCTGGTTGCGGCGGCGGCGTTGCCGCGGCGACTCTCGCAAGTTCTGGCCAGAAAGTGGTGGTTCTCGAGAAAGGGAACTATTTCACTGCTTCTGATTATTCATCTTTTGAAGGCCCTTCCCTCGATCAACTGTACGAAGCTGGTGGGGTTTTCACTAGTGATGATGGGAAAATGTTGATTCTGGCTGGTTCGACCGTCGGCGGCGGCTCCGCCGTTAATTGGTCGGCGAGCATTAGAACCCCAAATGCTGTTTTGCAGGAATGGGCTGAGAGTCACAAGATTCCGTTCTTTGGGAGCTCTGAATATCAGGTCGCCATGGATGCTGTGTGCAAGAGGATTGGGGTTTCAGAGGATTGTGAAGAAGAGGGGTTTCAGAATCAAGTTCTTCGAAAAGGGTGTGAAAATTTGGGACTTAAAGTTGAAAAAGTACCAAGAAATTCCCCAAATGGTCATTATTGTGGATCATGTGGTTATGGTTGTAGAAGAGGGGAAAAACGAGGGACTGATTCTACTTGGCTTGTTGATGCTGTGAATCATGGGGCAGTAATCATAACAAATTGCAAAGCTGAGAGATTCATATTGGAAAAGAACAGAACTGGAAGTGTGAGGAAGAGCAAGTGCTTGGGAGTCATTGCAAATGTTTGGAGTGATAACATTACCAAGAAACTGCAAATCAAGGCCAAAGCTACTGTCTCAGCTTGTGGGGCTCTTTTGACACCACCATTGATGATATCAAGTGGGCTGAGGAACAAACACATTGGAAGAAATCTTCATCTCCACCCTGTTTTGATGACTTGGGGATACTTTCCAGAGTCAAATTCAGAGTTCAAAGGGAAATCCTATGAAGGTGGAATAATCACATCACTCCACAAGGTTGTCTCTGAAGAATCCAAATCAAATCCCAAAACCATCATAGAGACGCCTTTATTAGGACCAGGATCATTTGCTGTTTTGTTTCCTTGGATATCTGGACTTGACACTAGGAAAAGAATGCTCCAGTATTCAAGAATTGCCCATTTCATAACGATAGTCCGAGATTCGGGATCCGGGGTAGTGAGGTCAGAGGGAAGGGTGAGCTATAATTTGAGTGAAGAAGACAGGGAAAACCTCAAGAGGGGGCTGAGGCAGGCATTGAGGATCTTGGTAGCTGCAGGAGCTACTGAAGTTGGCACACATAGAAGTGATGGACAGAGACTTGAATGTAAAGGGATTGGGAAGGAGGAATTGGAAGAGTTTTTGGATACGGTATGTGCAGAGAGAGGGCAACTATCAATGACAGAAAACTGGAATATATATACCTCAGCTCATCAGATGGGGAGCTGCAAAATGGGAGTTTCTGAAAAAGAAGGCGCTGTCGATGAAAACGGAGAGAGCTGGGAAGCTGAAGGCCTCTTTGTTTGTGATGCTAGTGTTCTTCCAACTGCTGTTGGTGTTAATCCCATGATTACCATTCAATCCACTGCTTACTGTATTTCAAACAGATTAGCAGCATTGTTAAGAAAGTAG

mRNA sequence

CAGGCGGTACTGGTAGGGCTGAGCATGATGAGAGAATGGGAATCAAAAAACAGAAAGGTAATCAAATTTACCAAATAGCCCTTGAATTTCCAAACCATTCCTATATATAGAGAGCTCACGTCTTCCGGCAAAGGCATATCGAGTTCATCTGCAGAGAAACACGATGCGGAGATCGGAGTGTCATCCTCTGTTGAGAGGAACAAGGAGCGATAAGTTCAGCCATGGCTTCTCTGCGGCGGAGATGGACTCATTGGCCTCCATCTGCGACGCCGTTCTTCCTCCTCTGCCGTTCTATTCACCAAACAACGGCCGCGATGAAATCGATGCTTCCGAGGCCGCCGCCGTGAAGGCGTTTTACGCGGCGTCCGGTTCGCAATCTCCGATTCCAGACGAGGTTGCGGAGACGGTGACGAAGAGAGGATTGATTGAAATTGTGATAGTTATAAGGGCGGTGCTGTGGATTCTGGCTACGAGGTTAGGGACGTTGTTGCTCTGTGGATCGCTTTGTTTCTGCGAGAAATGGCCTTTCGTCTGCAATTTCTCGGCTATGCCATTGGAGAAGAGAGAGGAAGCTTTGAGGAGATGGTTTAAGAATAAGGTTTTCACGCCCATTAGAACTGCTCTTGTTGGCGTCAGAGTTCTTTCCCTCTATATCTTCTTTTCTCGGGTTGATGTAAATGGCGATAACTCAAGTTGGGAAGCCATAAAATACCGGCCGGCCATTGATGAAAATCCGTCCAAATCCTCCGACGAGAGGCCTCTCCAGAAGGGAATCATAGAACCCAACAACGTAACCGAATCATCCTTTCGAAAATCCCTCTTACAGAAAGGTCTCAAAGTCACCGGAGACGACGGCCACACCCTTTCCATCGAATGCGATGTGGTGGTTGTTGGGTCTGGTTGCGGCGGCGGCGTTGCCGCGGCGACTCTCGCAAGTTCTGGCCAGAAAGTGGTGGTTCTCGAGAAAGGGAACTATTTCACTGCTTCTGATTATTCATCTTTTGAAGGCCCTTCCCTCGATCAACTGTACGAAGCTGGTGGGGTTTTCACTAGTGATGATGGGAAAATGTTGATTCTGGCTGGTTCGACCGTCGGCGGCGGCTCCGCCGTTAATTGGTCGGCGAGCATTAGAACCCCAAATGCTGTTTTGCAGGAATGGGCTGAGAGTCACAAGATTCCGTTCTTTGGGAGCTCTGAATATCAGGTCGCCATGGATGCTGTGTGCAAGAGGATTGGGGTTTCAGAGGATTGTGAAGAAGAGGGGTTTCAGAATCAAGTTCTTCGAAAAGGGTGTGAAAATTTGGGACTTAAAGTTGAAAAAGTACCAAGAAATTCCCCAAATGGTCATTATTGTGGATCATGTGGTTATGGTTGTAGAAGAGGGGAAAAACGAGGGACTGATTCTACTTGGCTTGTTGATGCTGTGAATCATGGGGCAGTAATCATAACAAATTGCAAAGCTGAGAGATTCATATTGGAAAAGAACAGAACTGGAAGTGTGAGGAAGAGCAAGTGCTTGGGAGTCATTGCAAATGTTTGGAGTGATAACATTACCAAGAAACTGCAAATCAAGGCCAAAGCTACTGTCTCAGCTTGTGGGGCTCTTTTGACACCACCATTGATGATATCAAGTGGGCTGAGGAACAAACACATTGGAAGAAATCTTCATCTCCACCCTGTTTTGATGACTTGGGGATACTTTCCAGAGTCAAATTCAGAGTTCAAAGGGAAATCCTATGAAGGTGGAATAATCACATCACTCCACAAGGTTGTCTCTGAAGAATCCAAATCAAATCCCAAAACCATCATAGAGACGCCTTTATTAGGACCAGGATCATTTGCTGTTTTGTTTCCTTGGATATCTGGACTTGACACTAGGAAAAGAATGCTCCAGTATTCAAGAATTGCCCATTTCATAACGATAGTCCGAGATTCGGGATCCGGGGTAGTGAGGTCAGAGGGAAGGGTGAGCTATAATTTGAGTGAAGAAGACAGGGAAAACCTCAAGAGGGGGCTGAGGCAGGCATTGAGGATCTTGGTAGCTGCAGGAGCTACTGAAGTTGGCACACATAGAAGTGATGGACAGAGACTTGAATGTAAAGGGATTGGGAAGGAGGAATTGGAAGAGTTTTTGGATACGGTATGTGCAGAGAGAGGGCAACTATCAATGACAGAAAACTGGAATATATATACCTCAGCTCATCAGATGGGGAGCTGCAAAATGGGAGTTTCTGAAAAAGAAGGCGCTGTCGATGAAAACGGAGAGAGCTGGGAAGCTGAAGGCCTCTTTGTTTGTGATGCTAGTGTTCTTCCAACTGCTGTTGGTGTTAATCCCATGATTACCATTCAATCCACTGCTTACTGTATTTCAAACAGATTAGCAGCATTGTTAAGAAAGTAG

Coding sequence (CDS)

ATGCGGAGATCGGAGTGTCATCCTCTGTTGAGAGGAACAAGGAGCGATAAGTTCAGCCATGGCTTCTCTGCGGCGGAGATGGACTCATTGGCCTCCATCTGCGACGCCGTTCTTCCTCCTCTGCCGTTCTATTCACCAAACAACGGCCGCGATGAAATCGATGCTTCCGAGGCCGCCGCCGTGAAGGCGTTTTACGCGGCGTCCGGTTCGCAATCTCCGATTCCAGACGAGGTTGCGGAGACGGTGACGAAGAGAGGATTGATTGAAATTGTGATAGTTATAAGGGCGGTGCTGTGGATTCTGGCTACGAGGTTAGGGACGTTGTTGCTCTGTGGATCGCTTTGTTTCTGCGAGAAATGGCCTTTCGTCTGCAATTTCTCGGCTATGCCATTGGAGAAGAGAGAGGAAGCTTTGAGGAGATGGTTTAAGAATAAGGTTTTCACGCCCATTAGAACTGCTCTTGTTGGCGTCAGAGTTCTTTCCCTCTATATCTTCTTTTCTCGGGTTGATGTAAATGGCGATAACTCAAGTTGGGAAGCCATAAAATACCGGCCGGCCATTGATGAAAATCCGTCCAAATCCTCCGACGAGAGGCCTCTCCAGAAGGGAATCATAGAACCCAACAACGTAACCGAATCATCCTTTCGAAAATCCCTCTTACAGAAAGGTCTCAAAGTCACCGGAGACGACGGCCACACCCTTTCCATCGAATGCGATGTGGTGGTTGTTGGGTCTGGTTGCGGCGGCGGCGTTGCCGCGGCGACTCTCGCAAGTTCTGGCCAGAAAGTGGTGGTTCTCGAGAAAGGGAACTATTTCACTGCTTCTGATTATTCATCTTTTGAAGGCCCTTCCCTCGATCAACTGTACGAAGCTGGTGGGGTTTTCACTAGTGATGATGGGAAAATGTTGATTCTGGCTGGTTCGACCGTCGGCGGCGGCTCCGCCGTTAATTGGTCGGCGAGCATTAGAACCCCAAATGCTGTTTTGCAGGAATGGGCTGAGAGTCACAAGATTCCGTTCTTTGGGAGCTCTGAATATCAGGTCGCCATGGATGCTGTGTGCAAGAGGATTGGGGTTTCAGAGGATTGTGAAGAAGAGGGGTTTCAGAATCAAGTTCTTCGAAAAGGGTGTGAAAATTTGGGACTTAAAGTTGAAAAAGTACCAAGAAATTCCCCAAATGGTCATTATTGTGGATCATGTGGTTATGGTTGTAGAAGAGGGGAAAAACGAGGGACTGATTCTACTTGGCTTGTTGATGCTGTGAATCATGGGGCAGTAATCATAACAAATTGCAAAGCTGAGAGATTCATATTGGAAAAGAACAGAACTGGAAGTGTGAGGAAGAGCAAGTGCTTGGGAGTCATTGCAAATGTTTGGAGTGATAACATTACCAAGAAACTGCAAATCAAGGCCAAAGCTACTGTCTCAGCTTGTGGGGCTCTTTTGACACCACCATTGATGATATCAAGTGGGCTGAGGAACAAACACATTGGAAGAAATCTTCATCTCCACCCTGTTTTGATGACTTGGGGATACTTTCCAGAGTCAAATTCAGAGTTCAAAGGGAAATCCTATGAAGGTGGAATAATCACATCACTCCACAAGGTTGTCTCTGAAGAATCCAAATCAAATCCCAAAACCATCATAGAGACGCCTTTATTAGGACCAGGATCATTTGCTGTTTTGTTTCCTTGGATATCTGGACTTGACACTAGGAAAAGAATGCTCCAGTATTCAAGAATTGCCCATTTCATAACGATAGTCCGAGATTCGGGATCCGGGGTAGTGAGGTCAGAGGGAAGGGTGAGCTATAATTTGAGTGAAGAAGACAGGGAAAACCTCAAGAGGGGGCTGAGGCAGGCATTGAGGATCTTGGTAGCTGCAGGAGCTACTGAAGTTGGCACACATAGAAGTGATGGACAGAGACTTGAATGTAAAGGGATTGGGAAGGAGGAATTGGAAGAGTTTTTGGATACGGTATGTGCAGAGAGAGGGCAACTATCAATGACAGAAAACTGGAATATATATACCTCAGCTCATCAGATGGGGAGCTGCAAAATGGGAGTTTCTGAAAAAGAAGGCGCTGTCGATGAAAACGGAGAGAGCTGGGAAGCTGAAGGCCTCTTTGTTTGTGATGCTAGTGTTCTTCCAACTGCTGTTGGTGTTAATCCCATGATTACCATTCAATCCACTGCTTACTGTATTTCAAACAGATTAGCAGCATTGTTAAGAAAGTAG

Protein sequence

MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAAVKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKWPFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEAIKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECDVVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVSEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDAVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACGALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSEESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEGRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTVCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAVGVNPMITIQSTAYCISNRLAALLRK
Homology
BLAST of Lcy10g014150 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 2.5e-282
Identity = 483/748 (64.57%), Postives = 583/748 (77.94%), Query Frame = 0

Query: 3   RSECHPLLRGTRSD-KFSHGFSAAEMDSLASICDAVLPPLPFYSPN-NGRDEIDASEAAA 62
           R ECHPLLRG R D KF  GFS++EM+SLASIC+ +LPPLP  +      D++   +   
Sbjct: 2   RKECHPLLRGGRKDNKFRQGFSSSEMESLASICEVILPPLPMDALKIKKHDDVSIED--- 61

Query: 63  VKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 122
           V+ F+  S S  PIP EVAE ++KR LIE VI+IR VLW+LATRLGT LLCG LCF EKW
Sbjct: 62  VEFFWNTSASHYPIPHEVAEILSKRSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKW 121

Query: 123 PFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEA 182
           PF+ NFS+M L+KRE  ++ W KN+  TPIR A   ++VL L+ +F+ VD NGDN +W+A
Sbjct: 122 PFINNFSSMSLKKRERVMQWWLKNRFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKA 181

Query: 183 IKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDD-GHTLSIECD 242
           I Y  + DE    SS+ R L+KGI+E  + T S+ ++SL +KGL VT D   + L ++CD
Sbjct: 182 IGYEVSADEKLPNSSNGRLLEKGIVETMHETNSTLQQSLTEKGLNVTLDSRNNILKVKCD 241

Query: 243 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 302
            +VVGSGCGGGVAA+ L+ +G KVVVLEKGNYF   DYSS EGPS+DQLYE GG+  S D
Sbjct: 242 AIVVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLYETGGILASVD 301

Query: 303 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 362
            ++L+LAGSTVGGGSAVNWSA I+TP+ VL+EW+E+H +  F +SEY  AM+ VC+RIGV
Sbjct: 302 SRILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSAMETVCERIGV 361

Query: 363 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 422
           +E C  EGFQNQVLRKGC+NLGLKV+ VPRNS   H+CGSCGYGC +GEK+GT  TWLVD
Sbjct: 362 TESCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEKQGTQDTWLVD 421

Query: 423 AVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACG 482
           AV +GAVIIT CKAERF+LE NR GS RK KCLGV+A   S  +T KLQI+AK T+SA G
Sbjct: 422 AVENGAVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQIEAKVTISAGG 481

Query: 483 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSE 542
           ALLTPPLMISSGL+NK+IG+NLHLHPVLMTWGYFPES SE KGK +EGGIITS+HKV S 
Sbjct: 482 ALLTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTSELKGKIFEGGIITSVHKVPSR 541

Query: 543 ESK--SNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVR 602
           +S   S+ + IIETP LGPGSFA L PW SGLD ++RML Y R AH ITI+RD   G V 
Sbjct: 542 DSNSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTAHLITIIRDKACGQVT 601

Query: 603 SEGRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFL 662
           +EGRVSY LS  D+EN+K GL+QALRIL AAGA EVGTHRSDGQR++C  + + E+ EF+
Sbjct: 602 TEGRVSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQRVKCGEVSENEMAEFI 661

Query: 663 DTVCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLP 722
           D+VC   G LS  ENWNIYTSAHQMGSC+MG++EKEGAVDENGESWEA+GLFVCDAS+LP
Sbjct: 662 DSVCPMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENGESWEAQGLFVCDASLLP 721

Query: 723 TAVGVNPMITIQSTAYCISNRLAALLRK 746
           TAVGVNPMITIQSTAYCISNR+   L++
Sbjct: 722 TAVGVNPMITIQSTAYCISNRVVDYLKR 746

BLAST of Lcy10g014150 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 6.6e-259
Identity = 450/724 (62.15%), Postives = 553/724 (76.38%), Query Frame = 0

Query: 23  SAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAAVKAFYAASGSQSPIPDEVAETV 82
           + AEM+SLAS C+AVLP +         +  +     A+++FY+ SGS++P+  +  E V
Sbjct: 16  TVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIELV 75

Query: 83  TKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKWPFVCNFSAMPLEKREEALRRWF 142
           TKRG IE  I  R +L++LATRLGTLL+CG+ C   +WPFV  FS + LEKRE  L++ F
Sbjct: 76  TKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQF 135

Query: 143 KNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEAIKYRPAIDEN-PSKSSDERPLQ 202
           KN + TPIR A V ++V  L+ FFSRV+ NG+N +WEAI YR   DEN PS++ +ERPL+
Sbjct: 136 KNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSETHNERPLE 195

Query: 203 KGIIEPNNVTESSFRKSLLQKGLKVTGDDGH-TLSIECDVVVVGSGCGGGVAAATLASSG 262
           KGI+E    TE +  +SL  KGL+   D  H  + I+CDVVVVGSG GGGVAA+ LA SG
Sbjct: 196 KGIVETMEETEQTLLESLAHKGLEAVLDTEHDAIRIKCDVVVVGSGSGGGVAASVLAKSG 255

Query: 263 QKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDGKMLILAGSTVGGGSAVNWSA 322
            KVVVLEKG+YFT S++  FEGP LD+LYE GG+  S DG  ++LAG+TVGGGSAVNWSA
Sbjct: 256 LKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATVGGGSAVNWSA 315

Query: 323 SIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVSEDCEEEGFQNQVLRKGCENL 382
            I+TP +VLQEW+E   IP FG+ EY  AM+ V KR+GV+E CE E FQNQ+LRKGCENL
Sbjct: 316 CIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQNQILRKGCENL 375

Query: 383 GLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDAVNHGAVIITNCKAERFILEK 442
           G  VE VPRNS   HYCGSCGYGCR+G+K+G+D TWLVDAV HGAVI+T CKAERFILEK
Sbjct: 376 GFNVENVPRNSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTGCKAERFILEK 435

Query: 443 NRTGSVRKS-KCLGVIANVWSDNITKKLQIKAKATVSACGALLTPPLMISSGLRNKHIGR 502
           N +    K  KCLGV+A   + NI K L+I+AK TVSA GALLTPPLMISSGLRN++IG+
Sbjct: 436 NGSNKGGKQMKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMISSGLRNRNIGK 495

Query: 503 NLHLHPVLMTWGYFPE---SNSEFKGKSYEGGIITSLHKVVSEESKSNPKTIIETPLLGP 562
           NLHLHPVLM WGYFP+   SN  FKG SYEGGIITS+ KV+SE+S+   + IIETP LGP
Sbjct: 496 NLHLHPVLMAWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSE--VRAIIETPQLGP 555

Query: 563 GSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEGRVSYNLSEEDRENLKR 622
           GSF+VL PW SGLD +KRM +YSR A  ITIVRD GSG V++EGR++Y + + DR+NLK 
Sbjct: 556 GSFSVLTPWTSGLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKA 615

Query: 623 GLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTVCAERGQLSMTENWNIY 682
           GLR++LRIL+AAGA EVGTHRSDGQRL CKG+ +  ++EFLD+V  E G   MTE WN+Y
Sbjct: 616 GLRESLRILIAAGAEEVGTHRSDGQRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVY 675

Query: 683 TSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAVGVNPMITIQSTAYCIS 741
           +SAHQMGSC++G +EKEGA+D NGESWEAE LFVCDAS LP+AVGVNPMIT+ STAYCIS
Sbjct: 676 SSAHQMGSCRIGENEKEGAIDLNGESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCIS 735

BLAST of Lcy10g014150 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 2.7e-252
Identity = 434/749 (57.94%), Postives = 562/749 (75.03%), Query Frame = 0

Query: 5   ECHPLLRGTRSDK--FSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAAVK 64
           E HPLL+G R  +  +SHG S+++M  +A+IC+A+ P  P  S NN      +S   A+ 
Sbjct: 8   ETHPLLKGGRRKEKGYSHGLSSSQMHVIAAICEALFPSQPLDSQNN-----QSSVDKALS 67

Query: 65  AFYAASGSQSPIPDEVAETVTK--RGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 124
           AFY ASGSQ+P+PDE AE + K  R   E + ++  VL IL+ RLGTLLLCG+LC   +W
Sbjct: 68  AFYTASGSQAPLPDEAAELLFKFNRSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWRW 127

Query: 125 PFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEA 184
           PF+  FS +PLEKREE L+RW + K + P+R   V  +++  Y  FSR DVNG N  W+A
Sbjct: 128 PFIHKFSEIPLEKREEILKRWSREKCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKA 187

Query: 185 IKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGD-DGHTLSIECD 244
           I Y+  +D     +  +RPLQ+G+IE    T+S+  +SL +KGL+VT D + +   I+CD
Sbjct: 188 IGYQ--VDTREKLTQKKRPLQEGLIETMYETDSTLIQSLTEKGLEVTEDLEQNMYKIKCD 247

Query: 245 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 304
            V+VGSGCGGGVAAA LA+SG KV++LEKG YF + DYSS EGPS+++LYE+GG+  S D
Sbjct: 248 AVIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESGGILPSLD 307

Query: 305 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 364
           GKM+ILAGST+GG SA+NWSA IRTP++VL+EW+E HKIP F S +YQ AMD VC+RIGV
Sbjct: 308 GKMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDTVCRRIGV 367

Query: 365 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 424
           +E+C +E FQNQ+LR+GC  +G KVE V  NS   HYCGSC YGCR G+K+GT+STWLVD
Sbjct: 368 TENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGTESTWLVD 427

Query: 425 AVNHGAVIITNCKAER--FILEKNRTGSVRKSKCLGVIAN-VWSDNITKKLQIKAKATVS 484
           AV +GAVI+T CKAE+  F L+    G+ RK+ C GVIA+  W   +TKKLQI++K T+S
Sbjct: 428 AVGNGAVILTGCKAEKLNFTLKDGDNGTKRKT-CSGVIASATWRSKVTKKLQIESKVTIS 487

Query: 485 ACGALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKV 544
           ACG+L TPPLMISSGL+N +IG+NLHLHP    WGYFPE  + F G +YEGGIITS+HKV
Sbjct: 488 ACGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNFSGNNYEGGIITSIHKV 547

Query: 545 VSEESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVV 604
             E+S S P+ IIE P LGPGSF+ L PW+SGLD ++RM++Y+R A+   +VRD GSG V
Sbjct: 548 FEEDSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYARTANLFALVRDHGSGEV 607

Query: 605 RSEGRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEF 664
           ++EGR+SY L + DRE+L+ GLR+ALRILVAAGA EVGT+RSDGQR++C+GI + +LEEF
Sbjct: 608 KAEGRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRSDGQRIKCRGIKESDLEEF 667

Query: 665 LDTVCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVL 724
           LD+V    G  S  E W ++TSAHQM SC+M  +E+EGAVDENGESWEA+GL+VCD SVL
Sbjct: 668 LDSVRVVGGPSSRNEVWTVFTSAHQMTSCRMSATEEEGAVDENGESWEAKGLYVCDGSVL 727

Query: 725 PTAVGVNPMITIQSTAYCISNRLAALLRK 746
           P+AVGVNPMITIQSTAYCI++ +A  L+K
Sbjct: 728 PSAVGVNPMITIQSTAYCIASNIAESLKK 748

BLAST of Lcy10g014150 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 826.6 bits (2134), Expect = 2.2e-238
Identity = 435/754 (57.69%), Postives = 534/754 (70.82%), Query Frame = 0

Query: 2   RRSECHPLLRG-TRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAA 61
           RR+  HPLLR   R + ++HGFS +++ SLA IC   LPP               SE  A
Sbjct: 6   RRNRGHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPP------------ETTSEQQA 65

Query: 62  VKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 121
           V +F+ AS +Q P  DEVAE + K G  E V V+R +L IL+ R GTLLLCGSLC  + W
Sbjct: 66  VNSFHVASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSW 125

Query: 122 PFVCNFSAMPLEKREEALRRWFKNKVF-TPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 181
           PFV  FS +PL+KRE  LR W +   F  P R      +  +L+ FFS+ D N  N + E
Sbjct: 126 PFVLKFSQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALE 185

Query: 182 AIKYRPAID-----ENPSKSSDE--RPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDG- 241
           AI Y   ID      N    +DE  RPL+KGIIE  + ++ +  +SL +KG+ V  DDG 
Sbjct: 186 AIGY--CIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVARDDGD 245

Query: 242 HTLSIECDVVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEA 301
           +   I CD VVVGSG GGGVAAA LA +G KV+VLEKGNYFTA DYS  E PS+ +LYE 
Sbjct: 246 NVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEK 305

Query: 302 GGVFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMD 361
           GG+ T+ DGK ++LAGS VGGG+AVNWSASIRTP+ VLQEW+E  KI FFGS EYQ AMD
Sbjct: 306 GGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMD 365

Query: 362 AVCKRIGVSEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRG 421
            V  RIGV+E C + GFQNQVLRKGCE LGL+VE VPRNSP  HYCG CGYGCR G K G
Sbjct: 366 EVTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNG 425

Query: 422 TDSTWLVDAVNHGAVIITNCKAERFILEKNRTGS-VRKSKCLGVIANVWSDNITKKLQIK 481
           TD TWLVDAV +GAVI+T  KAERF+L  N + S  RK +C+GV A+     I KK  I+
Sbjct: 426 TDQTWLVDAVENGAVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIE 485

Query: 482 AKATVSACGALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGII 541
           A+ TVS+ G+LLTPPLM+SSGL+N +IGRNL LHPVLMTWGYFPE +SEF GK YEGGII
Sbjct: 486 ARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFPEKDSEFSGKMYEGGII 545

Query: 542 TSLHKVVSEESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRD 601
           TS+H +   +++S  K I+E PL+GP S+A L PW+SG D ++RM++Y R AH   +VRD
Sbjct: 546 TSVHHM--NDTESGCKAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRD 605

Query: 602 SGSGVVRSEGRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGK 661
            GSG V  E  V+Y  +++DRENL+ GLRQALR+ VAAGA EVGT+RSDGQ+++C+ I K
Sbjct: 606 LGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITK 665

Query: 662 EELEEFLDTVCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFV 721
           E +EEFLD V A  G  +  E W  Y SAHQMGSC+MGV+ +EGA+DENGESWEAEGLFV
Sbjct: 666 EAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFV 725

Query: 722 CDASVLPTAVGVNPMITIQSTAYCISNRLAALLR 745
           CD S+LP+AVGVNPMITIQSTAYCIS+++   L+
Sbjct: 726 CDGSILPSAVGVNPMITIQSTAYCISSKIVDSLQ 743

BLAST of Lcy10g014150 vs. ExPASy Swiss-Prot
Match: Q9ZWB9 (Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=2)

HSP 1 Score: 785.4 bits (2027), Expect = 5.6e-226
Identity = 402/747 (53.82%), Postives = 531/747 (71.08%), Query Frame = 0

Query: 8   PLLR-GTRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAAVKAFYA 67
           PLLR   + + FSHGFS +++ +L+SICDA++PP+P  S N    ++      A+ +F+ 
Sbjct: 13  PLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNL-EMKLKVLRNDALLSFFK 72

Query: 68  ASGSQSPI-PDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK-WPFVC 127
           +S S+S + PDEVAE +  + +   V+V+R VL IL  RLGTLLLCG +C  +K WPF+ 
Sbjct: 73  SSSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFLL 132

Query: 128 NFSAMPLEKREEALRRWFKNKVFTPI-RTALVGVRVLSLYIFFSRVDVNGDNSSWEAIKY 187
            FS M LEKRE+ L+RW   + + P+ R   + ++ + L+ +F+  + N +N  W+AI Y
Sbjct: 133 KFSEMSLEKREKVLQRW-NTQWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAINY 192

Query: 188 RPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGD-DGHTLSIECDVVV 247
              I EN      ERPL +GIIE     E + ++ ++ KGLKVT D +  T  IECD VV
Sbjct: 193 SVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRERDTYKIECDAVV 252

Query: 248 VGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDGKM 307
           VGSGCGGGVAAA LA SG +VVV+EKGNYF   DYS+ EGPS+ +L+E+  +  + DG+ 
Sbjct: 253 VGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFELFESNSLMMTHDGRF 312

Query: 308 LILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVSED 367
             +AGSTVGGGS VNW+AS++TP+A+++EW+    I  + S +Y+ AM  VCKR+GV+E 
Sbjct: 313 RFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYKAAMGIVCKRLGVTEK 372

Query: 368 CEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDAVN 427
              EGFQNQ+LRKGCE LGL V  VPRNS   HYCGSC YGC  GEKRGTDSTWLVDAVN
Sbjct: 373 IIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAVN 432

Query: 428 HGAVIITNCKAERFILEKN----RTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSAC 487
           + AVI+T CKAE+ IL  N    R  S R+ +CLGV A++ S    KKLQI AK T+ AC
Sbjct: 433 NNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAASL-SHQTRKKLQINAKVTIVAC 492

Query: 488 GALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVS 547
           G+L TP L+ SSGL+N +I R LH+HP++M WGYFPE NSE +G ++EG I+TSLH V  
Sbjct: 493 GSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNSELEGAAHEGEIVTSLHYVHP 552

Query: 548 EESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRS 607
            +S + P   +ETP +GPG+FA L PW+SG D ++RM +Y+R AH   +VRD G G V+ 
Sbjct: 553 MDS-TTPNITLETPAIGPGTFAALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVKG 612

Query: 608 EGRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLD 667
           +  V Y L++ D ENL  GL+QALRILVAAGA EVGT+RSDGQR++C GI +++LE FLD
Sbjct: 613 D-IVKYRLTKADEENLTIGLKQALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLD 672

Query: 668 TVCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPT 727
           TV A  G +SM+++W    +AHQ+G C+MG +EKEGA+D  GESWEAE L+VCDASVLPT
Sbjct: 673 TVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVLPT 732

Query: 728 AVGVNPMITIQSTAYCISNRLAALLRK 746
           A+GVNPMIT+QSTAYCISNR+A L++K
Sbjct: 733 ALGVNPMITVQSTAYCISNRIAELMKK 754

BLAST of Lcy10g014150 vs. ExPASy TrEMBL
Match: A0A6J1EG06 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111432173 PE=3 SV=1)

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 653/746 (87.53%), Postives = 703/746 (94.24%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPF-YSPNNGRDEIDASEAA 60
           MRR ECHPLLRGTRS KF+HGFS AEM+SLA+ICD +LPPLP   S NNG DEID ++AA
Sbjct: 1   MRRLECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 60

Query: 61  AVKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK 120
           A++AFYAASGS+SPIPDEVAETV KRGL E+VI++R VLW+L TRLGTLLLCGSLCFC K
Sbjct: 61  AIEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 120

Query: 121 WPFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 180
           WP++CNFS+MPLE REEALRRW+KNKVFTPIR AL  +RVL LY FFSRVDVNG   +WE
Sbjct: 121 WPYICNFSSMPLENREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 181 AIKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECD 240
           AIKYRP I+EN SKSSDERPLQKGIIEPNN TESSF+KSLLQKGL+VTGDDG TLSI+CD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGDDGDTLSIQCD 240

Query: 241 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 300
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSS EGPSL+QLYEAGG+FTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 301 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 360
           GKMLILAGSTVGGGSAVNWSASIRTPN VLQEWAESHKIPFFG+SEYQ+AMDAVCKR+GV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 420
           SEDC+EEGFQNQVLRKGCENLGLKVEKVPRNSP+GHYCGSCGYGCRRGEK+GTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 421 AVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACG 480
           AVNHGAVIITNCKAERFILE+N+TGSVRKSKCLGV+ANVWSDN TKKLQIKAKAT+SACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 480

Query: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSE 540
           ALLTPP+MISSGLRNKHIGRNLHLHPVLM WGYFP+SNSEFKGKSYEGGIITSLHKV SE
Sbjct: 481 ALLTPPVMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVTSE 540

Query: 541 ESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSE 600
            S+SN KTIIETPLLGPGSF+VLFPWISGLDT++RML+YSRI+H ITIVRDSGSG VRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 600

Query: 601 GRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDT 660
           GRVS+NLSEEDRENLKRGLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEEL+EFLDT
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELDEFLDT 660

Query: 661 VCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTA 720
           +  E G LS+TENWNIYTSAHQMGSCKMGVSEK+GAV+ENGESWEAEGLFVCDASVLPTA
Sbjct: 661 ISVEGGPLSLTENWNIYTSAHQMGSCKMGVSEKDGAVNENGESWEAEGLFVCDASVLPTA 720

Query: 721 VGVNPMITIQSTAYCISNRLAALLRK 746
           VGVNPMITIQSTAYCISNRLAALL K
Sbjct: 721 VGVNPMITIQSTAYCISNRLAALLLK 746

BLAST of Lcy10g014150 vs. ExPASy TrEMBL
Match: A0A6J1IVY9 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479108 PE=3 SV=1)

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 651/746 (87.27%), Postives = 702/746 (94.10%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPF-YSPNNGRDEIDASEAA 60
           MRRSECHPLLRGTRS KF+HGFS AEM+SLA+ICD + PPLPF  S NNG DEID ++AA
Sbjct: 2   MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTIFPPLPFGSSNNNGSDEIDTADAA 61

Query: 61  AVKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK 120
           AV+AFYAASGS+SPIPDEVAETV KRGL E+VI++R VLW+L TRLGTLLLCGSLCFC K
Sbjct: 62  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 121

Query: 121 WPFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 180
           WP++CNFS+MPLE REEALRRWFKNKVFTPIR AL  +RVL LY FFSRVDVNG   +WE
Sbjct: 122 WPYICNFSSMPLENREEALRRWFKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 181

Query: 181 AIKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECD 240
           A+KYRP I+ENPSKSSDERPLQKGIIEPN  TESSF+KS LQKGL+VTGDDG+TLSI+CD
Sbjct: 182 AVKYRPGIEENPSKSSDERPLQKGIIEPNKETESSFKKSFLQKGLRVTGDDGYTLSIQCD 241

Query: 241 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 300
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSS EGPSL+QLYEAGG+FTS+D
Sbjct: 242 VVVVGSGCGGGVAAAFLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSND 301

Query: 301 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 360
           GKMLILAGSTVGGGSAVNWSASIRTPN VLQEWAESHKIPFFG+SEYQ AMDAVCKR+GV
Sbjct: 302 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQSAMDAVCKRLGV 361

Query: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 420
           SEDC+EEGFQNQVLRKGCENLGLKVEKVPRNSP+GHYCGSCGYGCRRGEK+GTDSTWLVD
Sbjct: 362 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 421

Query: 421 AVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACG 480
           AVNHGAVIITNCKAERFILE+N+TGSVRKSKCLGV+ANVWSDN TKKLQIKAKAT+ ACG
Sbjct: 422 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATILACG 481

Query: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSE 540
           ALLTPPLMISSGLRNKHIGRNLHLHPVLM WGYFP+SNSEFKGKSYEGGIITSLHKV+SE
Sbjct: 482 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVISE 541

Query: 541 ESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSE 600
            S+SN KTIIETP LGPGSF+VLFPWISGLDT++RML+YSRI+H ITIVRDSGSG VRSE
Sbjct: 542 GSESNSKTIIETPSLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 601

Query: 601 GRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDT 660
           GRVS+NLSEEDRENLKRGLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEEL+EFLD 
Sbjct: 602 GRVSFNLSEEDRENLKRGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELDEFLDM 661

Query: 661 VCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTA 720
           + AE G LS+TENWNIYTSAHQMGSCKMGVSEK+GAV+ENGESW+AEGLFVCDASVLPTA
Sbjct: 662 ISAEGGPLSLTENWNIYTSAHQMGSCKMGVSEKDGAVNENGESWDAEGLFVCDASVLPTA 721

Query: 721 VGVNPMITIQSTAYCISNRLAALLRK 746
           VGVNPMITIQSTAYCISNRLAALL K
Sbjct: 722 VGVNPMITIQSTAYCISNRLAALLLK 747

BLAST of Lcy10g014150 vs. ExPASy TrEMBL
Match: A0A5A7U0C1 (Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001810 PE=3 SV=1)

HSP 1 Score: 1311.6 bits (3393), Expect = 0.0e+00
Identity = 644/745 (86.44%), Postives = 691/745 (92.75%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAA 60
           MRRSECHPLLRGTR+ KF+HGFS AEM+SL SICD VLPPLPF SP         +  AA
Sbjct: 1   MRRSECHPLLRGTRTGKFTHGFSTAEMESLTSICDTVLPPLPFNSP--------TTSTAA 60

Query: 61  VKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 120
            +AFYA SG+QSPIPDEVAE   KRGLIEIV+++R VLWILATRLGTLLLCGSLCFCEKW
Sbjct: 61  ERAFYAVSGAQSPIPDEVAEMARKRGLIEIVLLVRVVLWILATRLGTLLLCGSLCFCEKW 120

Query: 121 PFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEA 180
           PFV  FS MPL+ REEALRRWFKNKVFTPIR AL  ++++SLYIFFSRVDVNGDN SWEA
Sbjct: 121 PFVWKFSEMPLKNREEALRRWFKNKVFTPIRAALALIKLVSLYIFFSRVDVNGDNPSWEA 180

Query: 181 IKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECDV 240
           IKY P IDE+PSKSSD RPLQKGIIEPNN TESSF+KSLLQKG+++ GDDGHTLS+ECDV
Sbjct: 181 IKYVPEIDEHPSKSSDVRPLQKGIIEPNNETESSFKKSLLQKGIRIAGDDGHTLSVECDV 240

Query: 241 VVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDG 300
           VVVGSGCGGGVAAA LASSGQKV+VLEKGNYFTASDYSS EGPSL+QLYEAGG+F +DDG
Sbjct: 241 VVVGSGCGGGVAAAILASSGQKVMVLEKGNYFTASDYSSLEGPSLEQLYEAGGIFATDDG 300

Query: 301 KMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVS 360
           KMLILAGSTVGGGSAVNWSASIRTP+ VLQEWAESHKIPFFG+SEYQVAMDAVCKRIGVS
Sbjct: 301 KMLILAGSTVGGGSAVNWSASIRTPDHVLQEWAESHKIPFFGTSEYQVAMDAVCKRIGVS 360

Query: 361 EDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDA 420
           EDCEEEGFQNQVLRKGCENLGLKVEKV RNSP GHYCGSCGYGCR+GEK+GTDSTWLVD+
Sbjct: 361 EDCEEEGFQNQVLRKGCENLGLKVEKVARNSPTGHYCGSCGYGCRKGEKQGTDSTWLVDS 420

Query: 421 VNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACGA 480
           VNHGAVIITNCKAERFIL +N+ GS+RKSKCLGVIANVWSDNITKKL IKAKAT+SACGA
Sbjct: 421 VNHGAVIITNCKAERFILARNKNGSMRKSKCLGVIANVWSDNITKKLHIKAKATISACGA 480

Query: 481 LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSEE 540
           LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPES+S FKGKSYEGGIITS+HKVVSEE
Sbjct: 481 LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESDSGFKGKSYEGGIITSVHKVVSEE 540

Query: 541 SKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEG 600
           SKSNPKTIIETPLLGPGS AVL PW+SGLDT++RML+YSR AHFITI+RDSGSG VRSEG
Sbjct: 541 SKSNPKTIIETPLLGPGSCAVLVPWVSGLDTKQRMLKYSRTAHFITILRDSGSGAVRSEG 600

Query: 601 RVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTV 660
           RVSYNLSEEDRENLK+GLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLD V
Sbjct: 601 RVSYNLSEEDRENLKKGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDNV 660

Query: 661 CAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 720
            AE G LS++ NWN+YTSAHQMGSCK+GVSEKEGAVDENGESWEAEGLFVCDASVLPTAV
Sbjct: 661 SAEGGLLSLSRNWNVYTSAHQMGSCKIGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 720

Query: 721 GVNPMITIQSTAYCISNRLAALLRK 746
           GVNPMITIQSTAYCISNR+AALLRK
Sbjct: 721 GVNPMITIQSTAYCISNRIAALLRK 737

BLAST of Lcy10g014150 vs. ExPASy TrEMBL
Match: A0A1S3AW60 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103483573 PE=3 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 643/745 (86.31%), Postives = 690/745 (92.62%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAA 60
           MRRSECHPLLRGTR+ KF+H FS AEM+SL SICD VLPPLPF SP         +  AA
Sbjct: 1   MRRSECHPLLRGTRTGKFTHEFSTAEMESLTSICDTVLPPLPFNSP--------TTSTAA 60

Query: 61  VKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 120
            +AFYA SG+QSPIPDEVAE   KRGL+EIV+++R VLWILATRLGTLLLCGSLCFCEKW
Sbjct: 61  ERAFYAVSGAQSPIPDEVAEMARKRGLVEIVLLVRVVLWILATRLGTLLLCGSLCFCEKW 120

Query: 121 PFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEA 180
           PFV  FS MPL+ REEALRRWFKNKVFTPIR AL  ++++SLYIFFSRVDVNGDN SWEA
Sbjct: 121 PFVWKFSEMPLKNREEALRRWFKNKVFTPIRAALALIKLVSLYIFFSRVDVNGDNPSWEA 180

Query: 181 IKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECDV 240
           IKY P IDE+PSKSSD RPLQKGIIEPNN TESSF+KSLLQKG+++ GDDGHTLS+ECDV
Sbjct: 181 IKYVPEIDEHPSKSSDVRPLQKGIIEPNNETESSFKKSLLQKGIRIAGDDGHTLSVECDV 240

Query: 241 VVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDG 300
           VVVGSGCGGGVAAA LASSGQKV+VLEKGNYFTASDYSS EGPSL+QLYEAGG+F +DDG
Sbjct: 241 VVVGSGCGGGVAAAILASSGQKVMVLEKGNYFTASDYSSLEGPSLEQLYEAGGIFATDDG 300

Query: 301 KMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVS 360
           KMLILAGSTVGGGSAVNWSASIRTP+ VLQEWAESHKIPFFG+SEYQVAMDAVCKRIGVS
Sbjct: 301 KMLILAGSTVGGGSAVNWSASIRTPDHVLQEWAESHKIPFFGTSEYQVAMDAVCKRIGVS 360

Query: 361 EDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDA 420
           EDCEEEGFQNQVLRKGCENLGLKVEKV RNSP GHYCGSCGYGCR+GEK+GTDSTWLVD+
Sbjct: 361 EDCEEEGFQNQVLRKGCENLGLKVEKVARNSPTGHYCGSCGYGCRKGEKQGTDSTWLVDS 420

Query: 421 VNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACGA 480
           VNHGAVIITNCKAERFIL +N+ GS+RKSKCLGVIANVWSDNITKKL IKAKAT+SACGA
Sbjct: 421 VNHGAVIITNCKAERFILARNKNGSMRKSKCLGVIANVWSDNITKKLHIKAKATISACGA 480

Query: 481 LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSEE 540
           LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPES+S FKGKSYEGGIITS+HKVVSEE
Sbjct: 481 LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESDSGFKGKSYEGGIITSVHKVVSEE 540

Query: 541 SKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEG 600
           SKSNPKTIIETPLLGPGS AVL PW+SGLDT++RML+YSR AHFITIVRDSGSG VRSEG
Sbjct: 541 SKSNPKTIIETPLLGPGSCAVLVPWVSGLDTKQRMLKYSRTAHFITIVRDSGSGAVRSEG 600

Query: 601 RVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTV 660
           RVSYNLSEEDRENLK+GLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLD V
Sbjct: 601 RVSYNLSEEDRENLKKGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDNV 660

Query: 661 CAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 720
            AE G LS++ NWN+YTSAHQMGSCK+GVSEKEGAVDENGESWEAEGLFVCDASVLPTAV
Sbjct: 661 SAEGGLLSLSRNWNVYTSAHQMGSCKIGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 720

Query: 721 GVNPMITIQSTAYCISNRLAALLRK 746
           GVNPMITIQSTAYCISNR+AALLRK
Sbjct: 721 GVNPMITIQSTAYCISNRIAALLRK 737

BLAST of Lcy10g014150 vs. ExPASy TrEMBL
Match: A0A6J1IBI0 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111473170 PE=3 SV=1)

HSP 1 Score: 1302.7 bits (3370), Expect = 0.0e+00
Identity = 637/745 (85.50%), Postives = 695/745 (93.29%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAA 60
           MRRSECHPLLRGTRS+KF+HGFSAAEM+S+ +ICD VLP LPF    + +DE +AS+ A+
Sbjct: 1   MRRSECHPLLRGTRSNKFTHGFSAAEMESITAICDTVLPSLPF----DSQDETNASDIAS 60

Query: 61  VKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 120
           V+AFY+ASGS+SPIPDEVAE +TK GLIEIVI+IR V+W+LATRLGTLLLCGSLCF EKW
Sbjct: 61  VEAFYSASGSRSPIPDEVAEMITKIGLIEIVILIRIVMWLLATRLGTLLLCGSLCFREKW 120

Query: 121 PFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEA 180
           PFVCNFSAM L+ REEAL+RW KNK+FT IR AL  +R+LSLYIFFSRVDVNGDN +WEA
Sbjct: 121 PFVCNFSAMALKNREEALKRWSKNKIFTFIRVALASIRILSLYIFFSRVDVNGDNPAWEA 180

Query: 181 IKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECDV 240
           IKYRPAIDENPSKSSDERPLQKGIIEPNN TESSF+ SLL+KGL V G++ HTLSIECDV
Sbjct: 181 IKYRPAIDENPSKSSDERPLQKGIIEPNNKTESSFQNSLLEKGLTVIGEENHTLSIECDV 240

Query: 241 VVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDG 300
           VV+GSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYS+FEGPSLDQLYEAGGV TS+DG
Sbjct: 241 VVIGSGCGGGVAAAILASSGQKVVVLEKGNYFTASDYSTFEGPSLDQLYEAGGVLTSNDG 300

Query: 301 KMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVS 360
           KM +LAGSTVGGGSAVNWSASIRTPNAVLQEW ES KIPFFGSSEYQ AMDAVCKRIGVS
Sbjct: 301 KMFVLAGSTVGGGSAVNWSASIRTPNAVLQEWVESDKIPFFGSSEYQNAMDAVCKRIGVS 360

Query: 361 EDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDA 420
           EDCE+EGFQNQVLRKGCENLG ++EKVPRNSPN HYCGSCGYGCR+GEK+GTD+TWLVDA
Sbjct: 361 EDCEQEGFQNQVLRKGCENLGFQIEKVPRNSPNTHYCGSCGYGCRKGEKQGTDTTWLVDA 420

Query: 421 VNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACGA 480
           VNHGAV+ITNCKAER ILE+NR GS+RK+KCLGVIA VWSDNITKKL+I+AKAT+SACGA
Sbjct: 421 VNHGAVVITNCKAERLILERNRAGSMRKNKCLGVIAKVWSDNITKKLKIRAKATISACGA 480

Query: 481 LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSEE 540
           LLTPPL+ISSGL+NKHIGRNLHLHPVLMTWGYFP+SNSEFKGKSYEGGIITS+HKVVSE 
Sbjct: 481 LLTPPLLISSGLKNKHIGRNLHLHPVLMTWGYFPDSNSEFKGKSYEGGIITSVHKVVSEG 540

Query: 541 SKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEG 600
           + SNPKTIIETPLLGPGSFA+LFPWISGLD++KRML+YSR AHFITIVRDSGSGVVRSEG
Sbjct: 541 TNSNPKTIIETPLLGPGSFAILFPWISGLDSKKRMLKYSRTAHFITIVRDSGSGVVRSEG 600

Query: 601 RVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTV 660
           RV YNLSEED+ENLK GLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEE+EEFLD V
Sbjct: 601 RVRYNLSEEDKENLKIGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEEVEEFLDMV 660

Query: 661 CAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 720
             E G L+MT NWNIYTSAHQMGSCKMGVSE +GAVDENGESWEAEGLFVCDASVLPTAV
Sbjct: 661 SVEGGPLTMTPNWNIYTSAHQMGSCKMGVSENDGAVDENGESWEAEGLFVCDASVLPTAV 720

Query: 721 GVNPMITIQSTAYCISNRLAALLRK 746
           GVNPMITIQSTAYCISNRLAALLRK
Sbjct: 721 GVNPMITIQSTAYCISNRLAALLRK 741

BLAST of Lcy10g014150 vs. NCBI nr
Match: XP_022924775.1 (long-chain-alcohol oxidase FAO1-like [Cucurbita moschata])

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 653/746 (87.53%), Postives = 703/746 (94.24%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPF-YSPNNGRDEIDASEAA 60
           MRR ECHPLLRGTRS KF+HGFS AEM+SLA+ICD +LPPLP   S NNG DEID ++AA
Sbjct: 1   MRRLECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 60

Query: 61  AVKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK 120
           A++AFYAASGS+SPIPDEVAETV KRGL E+VI++R VLW+L TRLGTLLLCGSLCFC K
Sbjct: 61  AIEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 120

Query: 121 WPFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 180
           WP++CNFS+MPLE REEALRRW+KNKVFTPIR AL  +RVL LY FFSRVDVNG   +WE
Sbjct: 121 WPYICNFSSMPLENREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 181 AIKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECD 240
           AIKYRP I+EN SKSSDERPLQKGIIEPNN TESSF+KSLLQKGL+VTGDDG TLSI+CD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGDDGDTLSIQCD 240

Query: 241 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 300
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSS EGPSL+QLYEAGG+FTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 301 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 360
           GKMLILAGSTVGGGSAVNWSASIRTPN VLQEWAESHKIPFFG+SEYQ+AMDAVCKR+GV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 420
           SEDC+EEGFQNQVLRKGCENLGLKVEKVPRNSP+GHYCGSCGYGCRRGEK+GTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 421 AVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACG 480
           AVNHGAVIITNCKAERFILE+N+TGSVRKSKCLGV+ANVWSDN TKKLQIKAKAT+SACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 480

Query: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSE 540
           ALLTPP+MISSGLRNKHIGRNLHLHPVLM WGYFP+SNSEFKGKSYEGGIITSLHKV SE
Sbjct: 481 ALLTPPVMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVTSE 540

Query: 541 ESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSE 600
            S+SN KTIIETPLLGPGSF+VLFPWISGLDT++RML+YSRI+H ITIVRDSGSG VRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 600

Query: 601 GRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDT 660
           GRVS+NLSEEDRENLKRGLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEEL+EFLDT
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELDEFLDT 660

Query: 661 VCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTA 720
           +  E G LS+TENWNIYTSAHQMGSCKMGVSEK+GAV+ENGESWEAEGLFVCDASVLPTA
Sbjct: 661 ISVEGGPLSLTENWNIYTSAHQMGSCKMGVSEKDGAVNENGESWEAEGLFVCDASVLPTA 720

Query: 721 VGVNPMITIQSTAYCISNRLAALLRK 746
           VGVNPMITIQSTAYCISNRLAALL K
Sbjct: 721 VGVNPMITIQSTAYCISNRLAALLLK 746

BLAST of Lcy10g014150 vs. NCBI nr
Match: XP_022979358.1 (long-chain-alcohol oxidase FAO1-like [Cucurbita maxima])

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 651/746 (87.27%), Postives = 702/746 (94.10%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPF-YSPNNGRDEIDASEAA 60
           MRRSECHPLLRGTRS KF+HGFS AEM+SLA+ICD + PPLPF  S NNG DEID ++AA
Sbjct: 2   MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTIFPPLPFGSSNNNGSDEIDTADAA 61

Query: 61  AVKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK 120
           AV+AFYAASGS+SPIPDEVAETV KRGL E+VI++R VLW+L TRLGTLLLCGSLCFC K
Sbjct: 62  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 121

Query: 121 WPFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 180
           WP++CNFS+MPLE REEALRRWFKNKVFTPIR AL  +RVL LY FFSRVDVNG   +WE
Sbjct: 122 WPYICNFSSMPLENREEALRRWFKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 181

Query: 181 AIKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECD 240
           A+KYRP I+ENPSKSSDERPLQKGIIEPN  TESSF+KS LQKGL+VTGDDG+TLSI+CD
Sbjct: 182 AVKYRPGIEENPSKSSDERPLQKGIIEPNKETESSFKKSFLQKGLRVTGDDGYTLSIQCD 241

Query: 241 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 300
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSS EGPSL+QLYEAGG+FTS+D
Sbjct: 242 VVVVGSGCGGGVAAAFLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSND 301

Query: 301 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 360
           GKMLILAGSTVGGGSAVNWSASIRTPN VLQEWAESHKIPFFG+SEYQ AMDAVCKR+GV
Sbjct: 302 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQSAMDAVCKRLGV 361

Query: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 420
           SEDC+EEGFQNQVLRKGCENLGLKVEKVPRNSP+GHYCGSCGYGCRRGEK+GTDSTWLVD
Sbjct: 362 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 421

Query: 421 AVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACG 480
           AVNHGAVIITNCKAERFILE+N+TGSVRKSKCLGV+ANVWSDN TKKLQIKAKAT+ ACG
Sbjct: 422 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATILACG 481

Query: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSE 540
           ALLTPPLMISSGLRNKHIGRNLHLHPVLM WGYFP+SNSEFKGKSYEGGIITSLHKV+SE
Sbjct: 482 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVISE 541

Query: 541 ESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSE 600
            S+SN KTIIETP LGPGSF+VLFPWISGLDT++RML+YSRI+H ITIVRDSGSG VRSE
Sbjct: 542 GSESNSKTIIETPSLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 601

Query: 601 GRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDT 660
           GRVS+NLSEEDRENLKRGLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEEL+EFLD 
Sbjct: 602 GRVSFNLSEEDRENLKRGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELDEFLDM 661

Query: 661 VCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTA 720
           + AE G LS+TENWNIYTSAHQMGSCKMGVSEK+GAV+ENGESW+AEGLFVCDASVLPTA
Sbjct: 662 ISAEGGPLSLTENWNIYTSAHQMGSCKMGVSEKDGAVNENGESWDAEGLFVCDASVLPTA 721

Query: 721 VGVNPMITIQSTAYCISNRLAALLRK 746
           VGVNPMITIQSTAYCISNRLAALL K
Sbjct: 722 VGVNPMITIQSTAYCISNRLAALLLK 747

BLAST of Lcy10g014150 vs. NCBI nr
Match: XP_023527426.1 (long-chain-alcohol oxidase FAO1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 654/746 (87.67%), Postives = 701/746 (93.97%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPF-YSPNNGRDEIDASEAA 60
           MRRSECHPLLRGTRS KF+HGFS AEM+SLA+ICD +LPPLP   S NNG DEID ++AA
Sbjct: 1   MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGNDEIDTTDAA 60

Query: 61  AVKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK 120
           A++AFYA SGS+SPIPDEVAETV KRGL E+VI++R VLW+LATRLGTLLLCGSLCFC K
Sbjct: 61  AIEAFYAESGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLATRLGTLLLCGSLCFCRK 120

Query: 121 WPFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 180
           WP++CNFS+MPLE REEALRRW KNKVFTPIR AL  +RVL LY FFSRVDVNG   +WE
Sbjct: 121 WPYICNFSSMPLENREEALRRWCKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 181 AIKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECD 240
           AIKYRP I+EN SKSSDERPLQKGIIEPNN TESSF+KSLLQKGL+VTG DG TLSI+CD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGHDGDTLSIQCD 240

Query: 241 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 300
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSS EGPSL+QLYEAGG+FTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 301 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 360
           GKMLILAGSTVGGGSAVNWSASIRTPN VLQEWAESHKIPFFG+SEYQ+AMDAVCKR+GV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 420
           SEDC+EEGFQNQVLRKGCENLGLKVEKVPRNSP+GHYCGSCGYGCRRGEK+GTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 421 AVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACG 480
           AVNHGAVIITNCKAERFILE+N+TGSVRKSKCLGV ANVWSDN TKKLQIKAKAT+SACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVRANVWSDNFTKKLQIKAKATISACG 480

Query: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSE 540
           ALLTPPLMISSGLRNKHIGRNLHLHPVLM WGYFP+SNSEFKGKSYEGGIITSLHKV+SE
Sbjct: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVISE 540

Query: 541 ESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSE 600
            S+SN KTIIETPLLGPGSF+VLFPWISGLDT++RML+YSRI+H ITIVRD GSG VRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDLGSGAVRSE 600

Query: 601 GRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDT 660
           GRVS+NLSEEDRENLKRGLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEEL+EFLD 
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELDEFLDM 660

Query: 661 VCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTA 720
           V AE G LS+TENWNIYTSAHQMGSCKMGVSEK+GAV+ENGESWEAEGLFVCDASVLPTA
Sbjct: 661 VSAEGGPLSLTENWNIYTSAHQMGSCKMGVSEKDGAVNENGESWEAEGLFVCDASVLPTA 720

Query: 721 VGVNPMITIQSTAYCISNRLAALLRK 746
           VGVNPMITIQSTAYCISNRLAALL K
Sbjct: 721 VGVNPMITIQSTAYCISNRLAALLLK 746

BLAST of Lcy10g014150 vs. NCBI nr
Match: KAG6582399.1 (Long-chain-alcohol oxidase FAO1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 649/746 (87.00%), Postives = 701/746 (93.97%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPF-YSPNNGRDEIDASEAA 60
           M+RSECHPLLRGTRS KF+HGFS AEM+SLA+ICD +LPPLP   S NNG DEID ++AA
Sbjct: 1   MQRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 60

Query: 61  AVKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK 120
           AV+AFYAASGS+SPIPDEVAETV KRGL E+VI++R VLW+L TRLGTLLLCGSLCFC K
Sbjct: 61  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 120

Query: 121 WPFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 180
           WP++CNFS+MPL+ REEALRRW+KNKVFTPIR AL  +RVL LY FFSRVDVNG   +WE
Sbjct: 121 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 181 AIKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECD 240
           AIKYRP I+EN SKSSDERPLQKGIIEPNN TESSF+KSLLQKGL+VTGDD  TLSI+CD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 240

Query: 241 VVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDD 300
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSS EGPSL+QLYEAGG+FTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 301 GKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGV 360
           GKMLILAGSTVGGGSAVNWSASIRTPN VLQEWAESHKIPFFG+SEYQ+AMDAVCKR+GV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVD 420
           SEDC+EEGFQNQVLRKGCENLGL VEKVPRNSP+GHYCGSCGYGCRRGEK+GTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 421 AVNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACG 480
           AVNHGAVIITNCKAERFILE+N+TG VRKSKCLGV+ANVWSDN TKKLQIKAKAT+SACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 480

Query: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSE 540
           ALLTPPLMISSGLRNKHIGRNLHLHPVLM WGYFP+S SEFKGKSYEGGIITSLHKV+SE
Sbjct: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 540

Query: 541 ESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSE 600
            S+SN KTIIETPLLGPGSF+VLFPWISGLDT++RML+YSRI+H ITIVRDSGSG VRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 600

Query: 601 GRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDT 660
           GRVS+NLSEEDRENLKRGLRQ+LRILVAAGATEVGTHRSDGQRLECKGIGKEEL+EFLDT
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLRILVAAGATEVGTHRSDGQRLECKGIGKEELDEFLDT 660

Query: 661 VCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTA 720
           +  E G LS+TENWNIYTSAHQMGSCKMGVSEK+GAV+ENGESW+AEGLFVCDASVLPTA
Sbjct: 661 ISVEGGPLSLTENWNIYTSAHQMGSCKMGVSEKDGAVNENGESWDAEGLFVCDASVLPTA 720

Query: 721 VGVNPMITIQSTAYCISNRLAALLRK 746
           VGVNPMITIQSTAYCISNRLAALL K
Sbjct: 721 VGVNPMITIQSTAYCISNRLAALLLK 746

BLAST of Lcy10g014150 vs. NCBI nr
Match: XP_038895948.1 (long-chain-alcohol oxidase FAO1 [Benincasa hispida])

HSP 1 Score: 1324.3 bits (3426), Expect = 0.0e+00
Identity = 659/745 (88.46%), Postives = 690/745 (92.62%), Query Frame = 0

Query: 1   MRRSECHPLLRGTRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAA 60
           M+  ECHPLLRGTRS KFSHGFSAAE+++LA+ICD +LPPLPF S NN      + +AAA
Sbjct: 1   MKTMECHPLLRGTRSGKFSHGFSAAEIETLATICDTILPPLPFDSSNNN----GSVDAAA 60

Query: 61  VKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 120
           VKAFYAASGSQS IPDEVAETV KRGLIE VI++R VLWILATRLGTLLLCGSLC CEKW
Sbjct: 61  VKAFYAASGSQSTIPDEVAETVRKRGLIETVIIVRLVLWILATRLGTLLLCGSLCLCEKW 120

Query: 121 PFVCNFSAMPLEKREEALRRWFKNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEA 180
           PFV  FS MPLE REEALRRWFKNKVFTPIR AL  +RVLSLYIFFSRVDVNGDN SWEA
Sbjct: 121 PFVRKFSEMPLENREEALRRWFKNKVFTPIRAALASLRVLSLYIFFSRVDVNGDNPSWEA 180

Query: 181 IKYRPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHTLSIECDV 240
           I YRP IDENPSKSSDERPLQ GII+PNN TESSF+K LLQKG+KVTGDDGHTL IECDV
Sbjct: 181 INYRPDIDENPSKSSDERPLQTGIIQPNNETESSFQKFLLQKGIKVTGDDGHTLYIECDV 240

Query: 241 VVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDG 300
           VVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSS EGPSLDQLYEAGGVF S+DG
Sbjct: 241 VVVGSGCGGGVAAAILASSGQKVVVLEKGNYFTASDYSSLEGPSLDQLYEAGGVFISNDG 300

Query: 301 KMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVS 360
           KMLILAGSTVGGGSAVNWSASIRTPN V+QEWAE+HKIPFFG+SEYQVAMD VCKRIGVS
Sbjct: 301 KMLILAGSTVGGGSAVNWSASIRTPNHVVQEWAENHKIPFFGTSEYQVAMDVVCKRIGVS 360

Query: 361 EDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDA 420
           EDCEEEGFQNQVLRKGCENLGLKVEKVPRNSP GHYCGSCGYGCRRGEK+GTDSTWLVDA
Sbjct: 361 EDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPTGHYCGSCGYGCRRGEKQGTDSTWLVDA 420

Query: 421 VNHGAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACGA 480
           VNHGAVIITNCKAERFILE+NR GSV+KSKCLGVI NVWSDN TKKLQIKAKAT+SACGA
Sbjct: 421 VNHGAVIITNCKAERFILERNRNGSVKKSKCLGVITNVWSDNFTKKLQIKAKATISACGA 480

Query: 481 LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVSEE 540
           LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITS+HKVVSEE
Sbjct: 481 LLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSVHKVVSEE 540

Query: 541 SKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEG 600
           SKSNPKTIIETPLLGPGS AVL PWISGLD ++RML+YSR AH ITIVRDSG G VRSEG
Sbjct: 541 SKSNPKTIIETPLLGPGSCAVLTPWISGLDAKQRMLKYSRTAHLITIVRDSGLGAVRSEG 600

Query: 601 RVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTV 660
           RVSYNLS+EDRENLK+GLRQ+LRILVAAGATEVGTHRSDGQ+LECKGIGKEELEEFL TV
Sbjct: 601 RVSYNLSKEDRENLKKGLRQSLRILVAAGATEVGTHRSDGQKLECKGIGKEELEEFLATV 660

Query: 661 CAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 720
            AE   LSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV
Sbjct: 661 SAEGDLLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 720

Query: 721 GVNPMITIQSTAYCISNRLAALLRK 746
           GVNPMITIQSTAYCISNR+A LL K
Sbjct: 721 GVNPMITIQSTAYCISNRIATLLMK 741

BLAST of Lcy10g014150 vs. TAIR 10
Match: AT3G23410.1 (fatty alcohol oxidase 3 )

HSP 1 Score: 894.8 bits (2311), Expect = 4.7e-260
Identity = 450/724 (62.15%), Postives = 553/724 (76.38%), Query Frame = 0

Query: 23  SAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAAVKAFYAASGSQSPIPDEVAETV 82
           + AEM+SLAS C+AVLP +         +  +     A+++FY+ SGS++P+  +  E V
Sbjct: 16  TVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIELV 75

Query: 83  TKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKWPFVCNFSAMPLEKREEALRRWF 142
           TKRG IE  I  R +L++LATRLGTLL+CG+ C   +WPFV  FS + LEKRE  L++ F
Sbjct: 76  TKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQF 135

Query: 143 KNKVFTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEAIKYRPAIDEN-PSKSSDERPLQ 202
           KN + TPIR A V ++V  L+ FFSRV+ NG+N +WEAI YR   DEN PS++ +ERPL+
Sbjct: 136 KNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSETHNERPLE 195

Query: 203 KGIIEPNNVTESSFRKSLLQKGLKVTGDDGH-TLSIECDVVVVGSGCGGGVAAATLASSG 262
           KGI+E    TE +  +SL  KGL+   D  H  + I+CDVVVVGSG GGGVAA+ LA SG
Sbjct: 196 KGIVETMEETEQTLLESLAHKGLEAVLDTEHDAIRIKCDVVVVGSGSGGGVAASVLAKSG 255

Query: 263 QKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDGKMLILAGSTVGGGSAVNWSA 322
            KVVVLEKG+YFT S++  FEGP LD+LYE GG+  S DG  ++LAG+TVGGGSAVNWSA
Sbjct: 256 LKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATVGGGSAVNWSA 315

Query: 323 SIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVSEDCEEEGFQNQVLRKGCENL 382
            I+TP +VLQEW+E   IP FG+ EY  AM+ V KR+GV+E CE E FQNQ+LRKGCENL
Sbjct: 316 CIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQNQILRKGCENL 375

Query: 383 GLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDAVNHGAVIITNCKAERFILEK 442
           G  VE VPRNS   HYCGSCGYGCR+G+K+G+D TWLVDAV HGAVI+T CKAERFILEK
Sbjct: 376 GFNVENVPRNSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTGCKAERFILEK 435

Query: 443 NRTGSVRKS-KCLGVIANVWSDNITKKLQIKAKATVSACGALLTPPLMISSGLRNKHIGR 502
           N +    K  KCLGV+A   + NI K L+I+AK TVSA GALLTPPLMISSGLRN++IG+
Sbjct: 436 NGSNKGGKQMKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMISSGLRNRNIGK 495

Query: 503 NLHLHPVLMTWGYFPE---SNSEFKGKSYEGGIITSLHKVVSEESKSNPKTIIETPLLGP 562
           NLHLHPVLM WGYFP+   SN  FKG SYEGGIITS+ KV+SE+S+   + IIETP LGP
Sbjct: 496 NLHLHPVLMAWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSE--VRAIIETPQLGP 555

Query: 563 GSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEGRVSYNLSEEDRENLKR 622
           GSF+VL PW SGLD +KRM +YSR A  ITIVRD GSG V++EGR++Y + + DR+NLK 
Sbjct: 556 GSFSVLTPWTSGLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKA 615

Query: 623 GLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTVCAERGQLSMTENWNIY 682
           GLR++LRIL+AAGA EVGTHRSDGQRL CKG+ +  ++EFLD+V  E G   MTE WN+Y
Sbjct: 616 GLRESLRILIAAGAEEVGTHRSDGQRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVY 675

Query: 683 TSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAVGVNPMITIQSTAYCIS 741
           +SAHQMGSC++G +EKEGA+D NGESWEAE LFVCDAS LP+AVGVNPMIT+ STAYCIS
Sbjct: 676 SSAHQMGSCRIGENEKEGAIDLNGESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCIS 735

BLAST of Lcy10g014150 vs. TAIR 10
Match: AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 826.6 bits (2134), Expect = 1.6e-239
Identity = 435/754 (57.69%), Postives = 534/754 (70.82%), Query Frame = 0

Query: 2   RRSECHPLLRG-TRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAA 61
           RR+  HPLLR   R + ++HGFS +++ SLA IC   LPP               SE  A
Sbjct: 6   RRNRGHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPP------------ETTSEQQA 65

Query: 62  VKAFYAASGSQSPIPDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKW 121
           V +F+ AS +Q P  DEVAE + K G  E V V+R +L IL+ R GTLLLCGSLC  + W
Sbjct: 66  VNSFHVASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSW 125

Query: 122 PFVCNFSAMPLEKREEALRRWFKNKVF-TPIRTALVGVRVLSLYIFFSRVDVNGDNSSWE 181
           PFV  FS +PL+KRE  LR W +   F  P R      +  +L+ FFS+ D N  N + E
Sbjct: 126 PFVLKFSQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALE 185

Query: 182 AIKYRPAID-----ENPSKSSDE--RPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDG- 241
           AI Y   ID      N    +DE  RPL+KGIIE  + ++ +  +SL +KG+ V  DDG 
Sbjct: 186 AIGY--CIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVARDDGD 245

Query: 242 HTLSIECDVVVVGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEA 301
           +   I CD VVVGSG GGGVAAA LA +G KV+VLEKGNYFTA DYS  E PS+ +LYE 
Sbjct: 246 NVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEK 305

Query: 302 GGVFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMD 361
           GG+ T+ DGK ++LAGS VGGG+AVNWSASIRTP+ VLQEW+E  KI FFGS EYQ AMD
Sbjct: 306 GGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMD 365

Query: 362 AVCKRIGVSEDCEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRG 421
            V  RIGV+E C + GFQNQVLRKGCE LGL+VE VPRNSP  HYCG CGYGCR G K G
Sbjct: 366 EVTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNG 425

Query: 422 TDSTWLVDAVNHGAVIITNCKAERFILEKNRTGS-VRKSKCLGVIANVWSDNITKKLQIK 481
           TD TWLVDAV +GAVI+T  KAERF+L  N + S  RK +C+GV A+     I KK  I+
Sbjct: 426 TDQTWLVDAVENGAVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIE 485

Query: 482 AKATVSACGALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGII 541
           A+ TVS+ G+LLTPPLM+SSGL+N +IGRNL LHPVLMTWGYFPE +SEF GK YEGGII
Sbjct: 486 ARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFPEKDSEFSGKMYEGGII 545

Query: 542 TSLHKVVSEESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRD 601
           TS+H +   +++S  K I+E PL+GP S+A L PW+SG D ++RM++Y R AH   +VRD
Sbjct: 546 TSVHHM--NDTESGCKAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRD 605

Query: 602 SGSGVVRSEGRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGK 661
            GSG V  E  V+Y  +++DRENL+ GLRQALR+ VAAGA EVGT+RSDGQ+++C+ I K
Sbjct: 606 LGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITK 665

Query: 662 EELEEFLDTVCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFV 721
           E +EEFLD V A  G  +  E W  Y SAHQMGSC+MGV+ +EGA+DENGESWEAEGLFV
Sbjct: 666 EAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFV 725

Query: 722 CDASVLPTAVGVNPMITIQSTAYCISNRLAALLR 745
           CD S+LP+AVGVNPMITIQSTAYCIS+++   L+
Sbjct: 726 CDGSILPSAVGVNPMITIQSTAYCISSKIVDSLQ 743

BLAST of Lcy10g014150 vs. TAIR 10
Match: AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 785.4 bits (2027), Expect = 4.0e-227
Identity = 402/747 (53.82%), Postives = 531/747 (71.08%), Query Frame = 0

Query: 8   PLLR-GTRSDKFSHGFSAAEMDSLASICDAVLPPLPFYSPNNGRDEIDASEAAAVKAFYA 67
           PLLR   + + FSHGFS +++ +L+SICDA++PP+P  S N    ++      A+ +F+ 
Sbjct: 13  PLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNL-EMKLKVLRNDALLSFFK 72

Query: 68  ASGSQSPI-PDEVAETVTKRGLIEIVIVIRAVLWILATRLGTLLLCGSLCFCEK-WPFVC 127
           +S S+S + PDEVAE +  + +   V+V+R VL IL  RLGTLLLCG +C  +K WPF+ 
Sbjct: 73  SSSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFLL 132

Query: 128 NFSAMPLEKREEALRRWFKNKVFTPI-RTALVGVRVLSLYIFFSRVDVNGDNSSWEAIKY 187
            FS M LEKRE+ L+RW   + + P+ R   + ++ + L+ +F+  + N +N  W+AI Y
Sbjct: 133 KFSEMSLEKREKVLQRW-NTQWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAINY 192

Query: 188 RPAIDENPSKSSDERPLQKGIIEPNNVTESSFRKSLLQKGLKVTGD-DGHTLSIECDVVV 247
              I EN      ERPL +GIIE     E + ++ ++ KGLKVT D +  T  IECD VV
Sbjct: 193 SVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRERDTYKIECDAVV 252

Query: 248 VGSGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDGKM 307
           VGSGCGGGVAAA LA SG +VVV+EKGNYF   DYS+ EGPS+ +L+E+  +  + DG+ 
Sbjct: 253 VGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFELFESNSLMMTHDGRF 312

Query: 308 LILAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVSED 367
             +AGSTVGGGS VNW+AS++TP+A+++EW+    I  + S +Y+ AM  VCKR+GV+E 
Sbjct: 313 RFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYKAAMGIVCKRLGVTEK 372

Query: 368 CEEEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDAVN 427
              EGFQNQ+LRKGCE LGL V  VPRNS   HYCGSC YGC  GEKRGTDSTWLVDAVN
Sbjct: 373 IIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAVN 432

Query: 428 HGAVIITNCKAERFILEKN----RTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSAC 487
           + AVI+T CKAE+ IL  N    R  S R+ +CLGV A++ S    KKLQI AK T+ AC
Sbjct: 433 NNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAASL-SHQTRKKLQINAKVTIVAC 492

Query: 488 GALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNSEFKGKSYEGGIITSLHKVVS 547
           G+L TP L+ SSGL+N +I R LH+HP++M WGYFPE NSE +G ++EG I+TSLH V  
Sbjct: 493 GSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNSELEGAAHEGEIVTSLHYVHP 552

Query: 548 EESKSNPKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRS 607
            +S + P   +ETP +GPG+FA L PW+SG D ++RM +Y+R AH   +VRD G G V+ 
Sbjct: 553 MDS-TTPNITLETPAIGPGTFAALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVKG 612

Query: 608 EGRVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLD 667
           +  V Y L++ D ENL  GL+QALRILVAAGA EVGT+RSDGQR++C GI +++LE FLD
Sbjct: 613 D-IVKYRLTKADEENLTIGLKQALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLD 672

Query: 668 TVCAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPT 727
           TV A  G +SM+++W    +AHQ+G C+MG +EKEGA+D  GESWEAE L+VCDASVLPT
Sbjct: 673 TVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVLPT 732

Query: 728 AVGVNPMITIQSTAYCISNRLAALLRK 746
           A+GVNPMIT+QSTAYCISNR+A L++K
Sbjct: 733 ALGVNPMITVQSTAYCISNRIAELMKK 754

BLAST of Lcy10g014150 vs. TAIR 10
Match: AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 577.8 bits (1488), Expect = 1.3e-164
Identity = 319/745 (42.82%), Postives = 460/745 (61.74%), Query Frame = 0

Query: 27  MDSLASICDAVLPPLPFYSPNNGRDEIDASEAAAVKAFYAASGSQSPIPDEVAETVTKRG 86
           M+SL +ICD  +  +     ++G   +D      V  +++AS SQ+  PD VA  +++R 
Sbjct: 1   MESLVAICDTFISSI----DDSGVGHVD----DCVAGYFSASASQTGTPDRVARLMSERL 60

Query: 87  LIEIVIVIRAVLWILATRLGTLLLCGSLCFCEKWPFVCNFSAMPLEKREEALRRWFKNKV 146
                 ++RA LW+L+T +G+L+LCG   F  ++P+   F  +P ++REE L  W  +  
Sbjct: 61  HHPKKWILRAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNW-SSSY 120

Query: 147 FTPIRTALVGVRVLSLYIFFSRVDVNGDNSSWEAIKYR-PAID----------ENPSKSS 206
           F+ +R     ++++S  +FF++VD  G N +W+AI Y  P+ D          E   K  
Sbjct: 121 FSLLRMLFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPDHSDHEVELNEEKKKKKP 180

Query: 207 DE--RPLQKGIIEPNNVTESSFRKSLLQKGLKVTGDDGHT---------LSIECDVVVVG 266
           +E   PL  GI++  +  E +  K L  +G  V+    +T         + I+CD VVVG
Sbjct: 181 EEIFGPLYNGIVDLKSPRE-AVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVG 240

Query: 267 SGCGGGVAAATLASSGQKVVVLEKGNYFTASDYSSFEGPSLDQLYEAGGVFTSDDGKMLI 326
           SG GGGVAA  LA +G KV+V+E GNY+  S  S  EG ++D +Y +GG+  + D  ++I
Sbjct: 241 SGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVI 300

Query: 327 LAGSTVGGGSAVNWSASIRTPNAVLQEWAESHKIPFFGSSEYQVAMDAVCKRIGVSEDCE 386
           LAGSTVGGGS +NWSASI+TP  V++EWAE  K+  FGS  Y+ AMD VCKR+GV     
Sbjct: 301 LAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFV 360

Query: 387 EEGFQNQVLRKGCENLGLKVEKVPRNSPNGHYCGSCGYGCRRGEKRGTDSTWLVDAV-NH 446
           EEGF N+VLRKGCE LGL V+ +PRN+P+ HYCG C  GC++G+K+GT  TWLVD V + 
Sbjct: 361 EEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESD 420

Query: 447 GAVIITNCKAERFILEKNRTGSVRKSKCLGVIANVWSDNITKKLQIKAKATVSACGALLT 506
             +I+  C+A   + +  +    +K K  GV A  + + I     ++++ T+ ACGAL T
Sbjct: 421 NGLILPGCQATEVMYDCEQG---KKKKATGV-AFAFGEEI---YVVESRVTIVACGALRT 480

Query: 507 PPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESNS--EFKGKSYEGGIITSLHKVVSEES 566
           P L+  SGL+N +IGRNL LHPV+M WG+FPE +   E K KSYEGGI+T++  VV EE+
Sbjct: 481 PHLLKRSGLKNSNIGRNLCLHPVVMAWGWFPEEDKWPEKKKKSYEGGIMTAMSSVVIEET 540

Query: 567 KSN-PKTIIETPLLGPGSFAVLFPWISGLDTRKRMLQYSRIAHFITIVRDSGSGVVRSEG 626
            S+  + +I+TP L PG F+ + PW S  D + RML++SR AH   ++RD G+G + S+ 
Sbjct: 541 HSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSKT 600

Query: 627 RVSYNLSEEDRENLKRGLRQALRILVAAGATEVGTHRSDGQRLECKGIGKEELEEFLDTV 686
            + YNL++ED E+LK GL + L+IL AAGA E+GTH S+G+ L  +     E+E F+   
Sbjct: 601 YIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERFV--- 660

Query: 687 CAERGQLSMTENWNIYTSAHQMGSCKMGVSEKEGAVDENGESWEAEGLFVCDASVLPTAV 746
             E     + +      SAHQMGSC+MG+  +E AV   GE+WE E LFV D SV PTA+
Sbjct: 661 -REESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTAL 720

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B5WWZ82.5e-28264.57Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1[more]
Q9LW566.6e-25962.15Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=... [more]
B5WWZ92.7e-25257.94Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1[more]
Q94BP32.2e-23857.69Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 S... [more]
Q9ZWB95.6e-22653.82Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1EG060.0e+0087.53Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111432173 PE=3 SV... [more]
A0A6J1IVY90.0e+0087.27Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479108 PE=3 SV=1[more]
A0A5A7U0C10.0e+0086.44Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3AW600.0e+0086.31Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103483573 PE=3 SV=1[more]
A0A6J1IBI00.0e+0085.50Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111473170 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022924775.10.0e+0087.53long-chain-alcohol oxidase FAO1-like [Cucurbita moschata][more]
XP_022979358.10.0e+0087.27long-chain-alcohol oxidase FAO1-like [Cucurbita maxima][more]
XP_023527426.10.0e+0087.67long-chain-alcohol oxidase FAO1-like [Cucurbita pepo subsp. pepo][more]
KAG6582399.10.0e+0087.00Long-chain-alcohol oxidase FAO1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038895948.10.0e+0088.46long-chain-alcohol oxidase FAO1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G23410.14.7e-26062.15fatty alcohol oxidase 3 [more]
AT4G28570.11.6e-23957.69Long-chain fatty alcohol dehydrogenase family protein [more]
AT1G03990.14.0e-22753.82Long-chain fatty alcohol dehydrogenase family protein [more]
AT4G19380.11.3e-16442.82Long-chain fatty alcohol dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 601..734
e-value: 1.1E-24
score: 87.5
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 239..506
e-value: 5.3E-68
score: 229.6
IPR012400Long-chain-alcohol oxidasePIRSFPIRSF028937Lg_Ch_AOcoord: 9..745
e-value: 0.0
score: 1037.6
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 226..539
e-value: 4.6E-27
score: 96.9
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 573..745
e-value: 1.6E-15
score: 59.2
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 236..744
NoneNo IPR availablePIRSRPIRSR000137-2PIRSR000137-2coord: 237..492
e-value: 1.1E-9
score: 35.0
NoneNo IPR availablePIRSRPIRSR028937-1PIRSR028937-1coord: 4..745
e-value: 0.0
score: 1205.2
NoneNo IPR availablePIRSRPIRSR028937-2PIRSR028937-2coord: 4..745
e-value: 0.0
score: 1205.2
NoneNo IPR availablePANTHERPTHR46056:SF10LONG-CHAIN-ALCOHOL OXIDASE FAO3coord: 3..744
NoneNo IPR availablePANTHERPTHR46056LONG-CHAIN-ALCOHOL OXIDASEcoord: 3..744

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g014150.1Lcy10g014150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046577 long-chain-alcohol oxidase activity
molecular_function GO:0046593 mandelonitrile lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors