Lcy10g013490 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy10g013490
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Proteinase inhibitor
Location	Chr10: 41668360 .. 41668950 (-)
RNA-Seq Expression	Lcy10g013490
Synteny	Lcy10g013490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCATAATTTTTGCCAACATGTGTGTAGGAAAGAGTTCGTGGCCGGAGTTGGTTGGTAGATCAGGAAATGAGGCAGAGAGGATTATTGAGTATGAAAACCCTTCGGTTGATGCTATAATTGTTGATTATGGGTTAGTAGTGTCTACTGACTTTAGGTGTGACAGGGTTTGGATTTGGGTAAGCAAAACGGGCATTGTCAATAGAACTCCATTTATTGGTTAGTCCTCAAAGAAACAGTCGGAAAGTAGATTAGTGTTTAGTACTGTACAAACGTGTTCATGTTGTTGTTCTTATATCTATGACACATGTGAATTATGAATAAGATGTTTGTATTATTGTCTTTGTTGTAATATAATAAAAATGTTTTTTATAGAACCACATCATTTCATTTCTTAATGATATGTCTATCTCTATGTCTTTCTTTCTTTTTTAATTATCATTTACCCTTAACACAAGGCAAGGAGAAGGCCTTCAACCACTTTCTTCTCCTCAAATTTGGTTACTTTCCTCAATTATCGGTTGAATTTTGCTATCATAAATCAAAGCTTCAAACAAGATGGAAAACTTATGGATATATGTGAAATATAA

mRNA sequence

ATGTTCATAATTTTTGCCAACATGTGTGTAGGAAAGAGTTCGTGGCCGGAGTTGGTTGGTAGATCAGGAAATGAGGCAGAGAGGATTATTGAGTATGAAAACCCTTCGGTTGATGCTATAATTGTTGATTATGGGTTAGTAGTGTCTACTGACTTTAGGTGTGACAGGGTTTGGATTTGGGCAAGGAGAAGGCCTTCAACCACTTTCTTCTCCTCAAATTTGGTTACTTTCCTCAATTATCGGTTGAATTTTGCTATCATAAATCAAAGCTTCAAACAAGATGGAAAACTTATGGATATATGTGAAATATAA

Coding sequence (CDS)

ATGTTCATAATTTTTGCCAACATGTGTGTAGGAAAGAGTTCGTGGCCGGAGTTGGTTGGTAGATCAGGAAATGAGGCAGAGAGGATTATTGAGTATGAAAACCCTTCGGTTGATGCTATAATTGTTGATTATGGGTTAGTAGTGTCTACTGACTTTAGGTGTGACAGGGTTTGGATTTGGGCAAGGAGAAGGCCTTCAACCACTTTCTTCTCCTCAAATTTGGTTACTTTCCTCAATTATCGGTTGAATTTTGCTATCATAAATCAAAGCTTCAAACAAGATGGAAAACTTATGGATATATGTGAAATATAA

Protein sequence

MFIIFANMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIWARRRPSTTFFSSNLVTFLNYRLNFAIINQSFKQDGKLMDICEI

Homology

[Close]

BLAST of Lcy10g013490 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 7.5e-12
Identity = 31/56 (55.36%), Postives = 41/56 (73.21%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIWARRR 65
          C GKSSWP LVG  G+ A+ IIE +NP+V A+I++ G  V+ DFRC+RV IW  +R
Sbjct: 3  CPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKR 58

[Close]

BLAST of Lcy10g013490 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 9.8e-12
Identity = 31/58 (53.45%), Postives = 39/58 (67.24%), Query Frame = 0


Query: 7  NMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIWARRR 65
          + C GK SWP+LVG +G  A+ +IE ENP V A+IV  G  V+ DFRCDRV +W   R
Sbjct: 1  SQCQGKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTER 58

[Close]

BLAST of Lcy10g013490 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.9e-11
Identity = 28/51 (54.90%), Postives = 38/51 (74.51%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWI 60
          C GK++WPELVG+SGN A   +E EN +V AI++  G  ++ DFRCDRVW+
Sbjct: 4  CPGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWV 54

[Close]

BLAST of Lcy10g013490 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 4.5e-09
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 0


Query: 6  ANMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWI 60
          A  C GK  WPELVG  G +A  IIE ENP+V +I+       + DFRCDRVW+
Sbjct: 1  ARECPGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWV 54

[Close]

BLAST of Lcy10g013490 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 2.1e-06
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 0


Query: 6  ANMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          A+ C  K+SWPELVG +G+ A  II+ EN +V AI+V  GL ++ D   +RV ++
Sbjct: 2  ASQCPVKNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVF 56

[Close]

BLAST of Lcy10g013490 vs. ExPASy TrEMBL
Match: A0A0A0L795 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.4e-16
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIWARRRPS 67
          C GKSSWPELVG++G EAE+IIE ENPSVDA+IVD G VV+ DFRCDRVW+W   + S
Sbjct: 5  CKGKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSKTS 62

[Close]

BLAST of Lcy10g013490 vs. ExPASy TrEMBL
Match: A0A5D3CZE2 (Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001160 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.3e-14
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          C GKSSWPELVG++G EAE+ IE ENP VDAIIVD G VV  DFRCDRVW+W
Sbjct: 5  CKGKSSWPELVGKAGKEAEKTIERENPLVDAIIVDEGSVVIQDFRCDRVWVW 56

[Close]

BLAST of Lcy10g013490 vs. ExPASy TrEMBL
Match: A0A5D3D207 (Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001170 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.9e-14
Identity = 39/54 (72.22%), Postives = 44/54 (81.48%), Query Frame = 0


Query: 7  NMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          N C GKSSWPELVG++G EAERIIE ENP V+A IV+ G VV  DFRCDRVW+W
Sbjct: 3  NPCKGKSSWPELVGKAGKEAERIIEKENPWVNADIVEEGSVVGQDFRCDRVWVW 56

[Close]

BLAST of Lcy10g013490 vs. ExPASy TrEMBL
Match: A0A7N2LVT1 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.9e-13
Identity = 39/63 (61.90%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 6  ANMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIWARRRP 65
          A+ C GK+SWPELVG  G  AE  IE ENPSVDAIIV+ G VV  DFRCDRVW+W  +  
Sbjct: 2  ASECEGKNSWPELVGVQGTVAEATIERENPSVDAIIVEEGSVVPQDFRCDRVWVWVDKNG 61

Query: 66 STT 69
            T
Sbjct: 62 IVT 64

[Close]

BLAST of Lcy10g013490 vs. ExPASy TrEMBL
Match: A0A1S4DTF7 (inhibitor of trypsin and hageman factor-like OS=Cucumis melo OX=3656 GN=LOC103483638 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-12
Identity = 37/52 (71.15%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          C GKSSWPELVG SGN AE+IIE EN  V+AIIV+ G  V+ DFRCDRVW+W
Sbjct: 5  CKGKSSWPELVGVSGNIAEKIIEKENHYVNAIIVEQGKFVTQDFRCDRVWVW 56

[Close]

BLAST of Lcy10g013490 vs. NCBI nr
Match: XP_004134090.1 (glu S.griseus protease inhibitor [Cucumis sativus])

HSP 1 Score: 95.5 bits (236), Expect = 2.8e-16
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIWARRRPS 67
          C GKSSWPELVG++G EAE+IIE ENPSVDA+IVD G VV+ DFRCDRVW+W   + S
Sbjct: 5  CKGKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSKTS 62

[Close]

BLAST of Lcy10g013490 vs. NCBI nr
Match: KAE8650342.1 (hypothetical protein Csa_009611 [Cucumis sativus])

HSP 1 Score: 95.5 bits (236), Expect = 2.8e-16
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIWARRRPS 67
          C GKSSWPELVG++G EAE+IIE ENPSVDA+IVD G VV+ DFRCDRVW+W   + S
Sbjct: 5  CKGKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSKTS 62

[Close]

BLAST of Lcy10g013490 vs. NCBI nr
Match: KAG6582359.1 (hypothetical protein SDJN03_22361, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 91.3 bits (225), Expect = 5.3e-15
Identity = 40/52 (76.92%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          C GKSSWPELVG+ G EAERIIE ENP VDAI+VD G VV  DFRCDRVW+W
Sbjct: 5  CKGKSSWPELVGKDGKEAERIIEKENPLVDAIVVDEGSVVIQDFRCDRVWVW 56

[Close]

BLAST of Lcy10g013490 vs. NCBI nr
Match: KAA0049295.1 (inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK17263.1 inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa])

HSP 1 Score: 89.0 bits (219), Expect = 2.6e-14
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          C GKSSWPELVG++G EAE+ IE ENP VDAIIVD G VV  DFRCDRVW+W
Sbjct: 5  CKGKSSWPELVGKAGKEAEKTIERENPLVDAIIVDEGSVVIQDFRCDRVWVW 56

[Close]

BLAST of Lcy10g013490 vs. NCBI nr
Match: KAA0049294.1 (inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK17264.1 inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa])

HSP 1 Score: 87.8 bits (216), Expect = 5.9e-14
Identity = 39/54 (72.22%), Postives = 44/54 (81.48%), Query Frame = 0


Query: 7  NMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          N C GKSSWPELVG++G EAERIIE ENP V+A IV+ G VV  DFRCDRVW+W
Sbjct: 3  NPCKGKSSWPELVGKAGKEAERIIEKENPWVNADIVEEGSVVGQDFRCDRVWVW 56

[Close]

BLAST of Lcy10g013490 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 62.0 bits (149), Expect = 3.2e-10
Identity = 26/50 (52.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 11 GKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          GKSSWPEL+G  G +A+ +IE ENP + A+I+  G VV   F C RV++W
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVW 85

[Close]

BLAST of Lcy10g013490 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 59.3 bits (142), Expect = 2.1e-09
Identity = 26/52 (50.00%), Postives = 37/52 (71.15%), Query Frame = 0


Query: 9  CVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          C  K+SWPEL G +G+ A  +IE ENP+V+A ++  G  V+ DFRCDRV ++
Sbjct: 5  CPRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVF 56

[Close]

BLAST of Lcy10g013490 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.7 bits (130), Expect = 5.2e-08
Identity = 23/56 (41.07%), Postives = 37/56 (66.07%), Query Frame = 0


Query: 5  FANMCVGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          F+   +GK+SWPEL+G +G+ A  +I+ EN S++ ++V  G  V+ D  C RV +W
Sbjct: 19 FSFFDIGKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVW 74

[Close]

BLAST of Lcy10g013490 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.3 bits (129), Expect = 6.7e-08
Identity = 22/51 (43.14%), Postives = 35/51 (68.63%), Query Frame = 0


Query: 10 VGKSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWIW 61
          +GK+SWPEL+G +G+ A  +I+ EN S++ ++V  G  V+ D  C RV +W
Sbjct: 9  IGKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVW 59

[Close]

BLAST of Lcy10g013490 vs. TAIR 10
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.4 bits (124), Expect = 2.6e-07
Identity = 25/48 (52.08%), Postives = 30/48 (62.50%), Query Frame = 0


Query: 12 KSSWPELVGRSGNEAERIIEYENPSVDAIIVDYGLVVSTDFRCDRVWI 60
          KSSWPELVGR G E + II+ EN  V A I+    VV     CDRV++
Sbjct: 14 KSSWPELVGRRGEEVKEIIDRENTKVTAKIISENAVVLAVVICDRVYV 61

The following BLAST results are available for this feature:

BLAST of Lcy10g013490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	7.5e-12	55.36	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P24076	9.8e-12	53.45	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P82381	4.9e-11	54.90	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P80211	4.5e-09	51.85	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
Q6XNP7	2.1e-06	45.45	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]

BLAST of Lcy10g013490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L795	1.4e-16	70.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1	[more]
A0A5D3CZE2	1.3e-14	75.00	Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5D3D207	2.9e-14	72.22	Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A7N2LVT1	1.9e-13	61.90	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]
A0A1S4DTF7	1.6e-12	71.15	inhibitor of trypsin and hageman factor-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]

BLAST of Lcy10g013490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134090.1	2.8e-16	70.69	glu S.griseus protease inhibitor [Cucumis sativus]	[more]
KAE8650342.1	2.8e-16	70.69	hypothetical protein Csa_009611 [Cucumis sativus]	[more]
KAG6582359.1	5.3e-15	76.92	hypothetical protein SDJN03_22361, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAA0049295.1	2.6e-14	75.00	inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK1726...	[more]
KAA0049294.1	5.9e-14	72.22	inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK1726...	[more]

BLAST of Lcy10g013490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	3.2e-10	52.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	2.1e-09	50.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	5.2e-08	41.07	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.1	6.7e-08	43.14	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43570.1	2.6e-07	52.08	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 12..61 e-value: 1.1E-18 score: 67.6
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 8..61
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 15..26
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 6..76 e-value: 1.3E-20 score: 75.4
None	No IPR available	PANTHER	PTHR33091:SF41	SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATED	coord: 8..61
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 8..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy10g013490.1	Lcy10g013490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity