Lcy10g012340 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g012340
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionbasic 7S globulin-like
LocationChr10: 40639800 .. 40642395 (+)
RNA-Seq ExpressionLcy10g012340
SyntenyLcy10g012340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCAAAAATTCTCAAGTCCCCTTCCAATGGCGACCTCCACTTCCCTCTGCTTGTTCTCCTCCATTCTCTTCCTCGTCTTCTCCACCTCCATTGCCGCAACCTCCTTCCGCCCCAAAGCCCTCGTCCTCCCCGTCACAAAGGACCCATCTCGGCAGTACATCACCCAGATTCACCAGCGGACTCCTCTGGTTCCGGTCAAGCTCACCGTCGACCTGGGCGGCCAGTTCATGTGGGTCGACTGTGACCGCGGCTACGTCTCTTCCACCTACAAGCCTGCCCGCTGCCGCTCGGCTCAGTGCTCTCTGGCTTCCAAGTCCAGCTCCTGCGGCCAGTGCTTTTCGCCGCCCAGGCCCGGCTGCAACAACAACACCTGCGGCCTCTTTCCGGGCAATACTATTATTCATCTCTCCACTAGCGGTGAAGTCGCCTCCGACGTCGTTTCTCTTTCCTCCACTGATGGCTTCAATCCGACCAGGTAAATTTTTATCTCTCCCTTTTCTTTTTGTTAATTTCAACTTTTTTTTTAAGCTTGGTTATGGTAATAATAATAATAATTATTATTATTTAATTTATGCTGGTTAATACAAAGCTCCTGTTGTTACTACAATTAAATTTTTTATGGAGTAGGGAATTTTAAAAAAAAAAAAATAAATTGAATGGTTAACTTCTTTTTTTAATGGGTGTGCGTGTTTGGAGGAAAATTCCTTCTACAATTTATGAACAATATTTGGCTGTATATAGGAAGTACACAGGTGTCTTTCTACATTTTCCATTAAAATTTTGACAAACAAGAAAGAGAGAAGTAGAAAATGAAAAATTTTCTTATCCTTCTTACTTGTCAACATTTTAAATTTTAATAGTGGTATATATCGGTATGCAACCAAGTTTTTTCATAATCAATTAGCAGGGGAATTTTGTTTCTTTAAAAAGTTATTATTCTAACTCCGAACCTACTGATCACTGTCGTTCTCAAAATAATAATAATAATAAATAAATAAATAAATAAATCACGTGTAAAAAATTTAAATTTTGAAATATTTATTTTTTTTAGTAAAATAATATATCTCTTTCTTTTCTTATTTATTTATTGAGGATATAATAATGGTAAGTAAATCTTTAATCTTTATAGTTAATCTAGCTTTAACAAATGAATTAAACACCAAGATGGGATTAGAATCTAATTTAGTAAAGAAGGAGAAAGAAAAGGAGTTCTGATTGACAATTTAAGAACCTGATTGGTTATAACATTATTTATTTATTTATTTATAATTTAATATCATCCGAAGGTGGGAAAATTCAAAATTCGAGAATATATATCTTAATCAATTAAATTAGATTCATTGATGGAAGGGTTTGAATCCCATTTTTTCTTTAAAGCTATTTGTTCAAACTAACATTAAAAATGAAAACTATTTTTTTTTTTTAAAATTCTAGGCTAACTTTGATATTTTAAACTTACATTAAAAAAAAAAAAAAAAAAACTATAGTTTAATACCACTTCAAATTTACACCCAATCTAAAATCTTAAAATTACAATAAGTTTTAATCTTTTATTCATGTTCTTTAACCCACCGCAGGGCCGTGTCGGTGCCGAACTTCCTCTTCGTCTGCGGCTCCACGTTTCTCCTCGAGGGTCTCGCCGGCGGCGTAAGCGGCATGGCCGGATTCGGAAGGACCGGAATCTCTCTGCCGTCCCAATTTGCAGCAGCCTTCAGCTTCAACAGAAAATTCGCCATTTGCCTGAGCGGCTCCACCAGATTCCCCGGCGTCATCTTCTCCGGCAACGGCCCATACCATTTCCTACCCAACATCGACCTCACCAAATCCCTCACTTACACCCCACTCTTCATCAACCCCGTCAGCACCGCCGGCGTCTTCTCCGCCGGCGAGAAATCCGCCGAATATTTCATCGGCGTCAAATCCATCGTCATCAACTCCAAAACCGTCCCCCTCAACACCACCCTCCTCAAAATCGACAGCAACGGCGTCGGCGGCACCAAAATCAGCACCGTCGACCCCTACACCGTTCTAGAATCGTCGATCTACAACGCCGTGTTGAAAACCTTCACGACGGAGCTCCGGCAGGTTCCGAGAGTGGCGGCGGTGGCGCCGTTTGGGGCTTGTTTCAAGGCGAAGAGCCTGTCGAGTACCCGGCTCGGGCCCGGCGTTCCGTCGATCGATTTGATTCTGCAGAACAAAAATGTGATTTGGAGAATCTTTGGTGCGAACTCGATGGTGCAGGTGAACGACGACGTTTTGTGCTTGGGATTCGTGGACGGTGGCGTTAACCCGCGAACGTCGATTGTTATTGGCGCCCACCAGATTGAAGATAATTTGGTCGAATTTGATTTGGCCACTTCCAGACTTGGATTTAGTTCCACTCTTTTGGGTCGGATGACTACTTGTGCTAATTTCAACTTTACTTCTAAAGCTTGATTAATTAATTTGAATTGAGTGATTAAAGTACCCATCAATAAATGTTGCTTCTTTTGAGGTTTGTATTGTATTTGTATTTGTATTGAATAATAATTTATGGATATGAAGCCATCGAAGCTTTATTATTTTAGGCATGTCGTGGCATCATGCCGATGCTTT

mRNA sequence

CACCAAAAATTCTCAAGTCCCCTTCCAATGGCGACCTCCACTTCCCTCTGCTTGTTCTCCTCCATTCTCTTCCTCGTCTTCTCCACCTCCATTGCCGCAACCTCCTTCCGCCCCAAAGCCCTCGTCCTCCCCGTCACAAAGGACCCATCTCGGCAGTACATCACCCAGATTCACCAGCGGACTCCTCTGGTTCCGGTCAAGCTCACCGTCGACCTGGGCGGCCAGTTCATGTGGGTCGACTGTGACCGCGGCTACGTCTCTTCCACCTACAAGCCTGCCCGCTGCCGCTCGGCTCAGTGCTCTCTGGCTTCCAAGTCCAGCTCCTGCGGCCAGTGCTTTTCGCCGCCCAGGCCCGGCTGCAACAACAACACCTGCGGCCTCTTTCCGGGCAATACTATTATTCATCTCTCCACTAGCGGTGAAGTCGCCTCCGACGTCGTTTCTCTTTCCTCCACTGATGGCTTCAATCCGACCAGGGCCGTGTCGGTGCCGAACTTCCTCTTCGTCTGCGGCTCCACGTTTCTCCTCGAGGGTCTCGCCGGCGGCGTAAGCGGCATGGCCGGATTCGGAAGGACCGGAATCTCTCTGCCGTCCCAATTTGCAGCAGCCTTCAGCTTCAACAGAAAATTCGCCATTTGCCTGAGCGGCTCCACCAGATTCCCCGGCGTCATCTTCTCCGGCAACGGCCCATACCATTTCCTACCCAACATCGACCTCACCAAATCCCTCACTTACACCCCACTCTTCATCAACCCCGTCAGCACCGCCGGCGTCTTCTCCGCCGGCGAGAAATCCGCCGAATATTTCATCGGCGTCAAATCCATCGTCATCAACTCCAAAACCGTCCCCCTCAACACCACCCTCCTCAAAATCGACAGCAACGGCGTCGGCGGCACCAAAATCAGCACCGTCGACCCCTACACCGTTCTAGAATCGTCGATCTACAACGCCGTGTTGAAAACCTTCACGACGGAGCTCCGGCAGGTTCCGAGAGTGGCGGCGGTGGCGCCGTTTGGGGCTTGTTTCAAGGCGAAGAGCCTGTCGAGTACCCGGCTCGGGCCCGGCGTTCCGTCGATCGATTTGATTCTGCAGAACAAAAATGTGATTTGGAGAATCTTTGGTGCGAACTCGATGGTGCAGGTGAACGACGACGTTTTGTGCTTGGGATTCGTGGACGGTGGCGTTAACCCGCGAACGTCGATTGTTATTGGCGCCCACCAGATTGAAGATAATTTGGTCGAATTTGATTTGGCCACTTCCAGACTTGGATTTAGTTCCACTCTTTTGGGTCGGATGACTACTTGTGCTAATTTCAACTTTACTTCTAAAGCTTGATTAATTAATTTGAATTGAGTGATTAAAGTACCCATCAATAAATGTTGCTTCTTTTGAGGTTTGTATTGTATTTGTATTTGTATTGAATAATAATTTATGGATATGAAGCCATCGAAGCTTTATTATTTTAGGCATGTCGTGGCATCATGCCGATGCTTT

Coding sequence (CDS)

ATGGCGACCTCCACTTCCCTCTGCTTGTTCTCCTCCATTCTCTTCCTCGTCTTCTCCACCTCCATTGCCGCAACCTCCTTCCGCCCCAAAGCCCTCGTCCTCCCCGTCACAAAGGACCCATCTCGGCAGTACATCACCCAGATTCACCAGCGGACTCCTCTGGTTCCGGTCAAGCTCACCGTCGACCTGGGCGGCCAGTTCATGTGGGTCGACTGTGACCGCGGCTACGTCTCTTCCACCTACAAGCCTGCCCGCTGCCGCTCGGCTCAGTGCTCTCTGGCTTCCAAGTCCAGCTCCTGCGGCCAGTGCTTTTCGCCGCCCAGGCCCGGCTGCAACAACAACACCTGCGGCCTCTTTCCGGGCAATACTATTATTCATCTCTCCACTAGCGGTGAAGTCGCCTCCGACGTCGTTTCTCTTTCCTCCACTGATGGCTTCAATCCGACCAGGGCCGTGTCGGTGCCGAACTTCCTCTTCGTCTGCGGCTCCACGTTTCTCCTCGAGGGTCTCGCCGGCGGCGTAAGCGGCATGGCCGGATTCGGAAGGACCGGAATCTCTCTGCCGTCCCAATTTGCAGCAGCCTTCAGCTTCAACAGAAAATTCGCCATTTGCCTGAGCGGCTCCACCAGATTCCCCGGCGTCATCTTCTCCGGCAACGGCCCATACCATTTCCTACCCAACATCGACCTCACCAAATCCCTCACTTACACCCCACTCTTCATCAACCCCGTCAGCACCGCCGGCGTCTTCTCCGCCGGCGAGAAATCCGCCGAATATTTCATCGGCGTCAAATCCATCGTCATCAACTCCAAAACCGTCCCCCTCAACACCACCCTCCTCAAAATCGACAGCAACGGCGTCGGCGGCACCAAAATCAGCACCGTCGACCCCTACACCGTTCTAGAATCGTCGATCTACAACGCCGTGTTGAAAACCTTCACGACGGAGCTCCGGCAGGTTCCGAGAGTGGCGGCGGTGGCGCCGTTTGGGGCTTGTTTCAAGGCGAAGAGCCTGTCGAGTACCCGGCTCGGGCCCGGCGTTCCGTCGATCGATTTGATTCTGCAGAACAAAAATGTGATTTGGAGAATCTTTGGTGCGAACTCGATGGTGCAGGTGAACGACGACGTTTTGTGCTTGGGATTCGTGGACGGTGGCGTTAACCCGCGAACGTCGATTGTTATTGGCGCCCACCAGATTGAAGATAATTTGGTCGAATTTGATTTGGCCACTTCCAGACTTGGATTTAGTTCCACTCTTTTGGGTCGGATGACTACTTGTGCTAATTTCAACTTTACTTCTAAAGCTTGA

Protein sequence

MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLTVDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFPGNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGFGRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLFINPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTVLESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVIWRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLLGRMTTCANFNFTSKA
Homology
BLAST of Lcy10g012340 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 5.7e-170
Identity = 303/439 (69.02%), Postives = 359/439 (81.78%), Query Frame = 0

Query: 1   MATSTSL--CLFSSILFLVFSTSIAATSFRPKALVLPVTKDP-SRQYITQIHQRTPLVPV 60
           MA+S  L   L  S+LF+  +T+ + TSFRPK L+LP+TKD  + QY+TQI QRTPLVPV
Sbjct: 1   MASSCCLHAILLCSLLFITSTTAQSETSFRPKGLILPITKDALTLQYLTQIQQRTPLVPV 60

Query: 61  KLTVDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCG 120
            LT+DLGGQF+WVDCD+GYVSSTY+PARCRSAQCSLA   S CGQCFSPP+PGCNNNTCG
Sbjct: 61  SLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQCSLAGAGSGCGQCFSPPKPGCNNNTCG 120

Query: 121 LFPGNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGM 180
           L P NTI   +TSGE+ASD V + S++G NP R VS  +FLFVCGSTFLLEGLA GV GM
Sbjct: 121 LLPDNTITRTATSGELASDTVQVQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGM 180

Query: 181 AGFGRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKS-LTY 240
           AG GRT ISLPSQF+A FSF RKFA+CLS ST   GV+  G+GPY FLPN +   +  +Y
Sbjct: 181 AGLGRTRISLPSQFSAEFSFPRKFAVCLSSSTNSKGVVLFGDGPYTFLPNREFANNDFSY 240

Query: 241 TPLFINPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVD 300
           TPLFINPVSTA  FS+ E S+EYFIGVKSI IN K VP+NTTLL ID+ GVGGTKISTV+
Sbjct: 241 TPLFINPVSTASAFSSREPSSEYFIGVKSIKINEKVVPINTTLLSIDNQGVGGTKISTVN 300

Query: 301 PYTVLESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQN 360
           PYT+LE+SIYNAV   F  EL  + RVA+VAPF ACF +++++STR+GP VPSIDL+LQN
Sbjct: 301 PYTILETSIYNAVTNFFVKELVNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQN 360

Query: 361 KNVIWRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFS 420
           +NV WRIFGANSMVQV+++VLCLGFVDGGV+PRTSIV+G + IEDNL++FDLA SRLGF+
Sbjct: 361 ENVFWRIFGANSMVQVSENVLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFT 420

Query: 421 STLLGRMTTCANFNFTSKA 436
           S++L R TTCANFNFTS A
Sbjct: 421 SSILFRQTTCANFNFTSIA 439

BLAST of Lcy10g012340 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 358.6 bits (919), Expect = 9.9e-98
Identity = 203/432 (46.99%), Postives = 265/432 (61.34%), Query Frame = 0

Query: 9   LFSSILFLVFSTSIAATS-----FRPKALVLPVTKD-PSRQYITQIHQRTPLVPVKLTVD 68
           LF   L L+  TS A  S      RP  LVL V KD  +  ++ QIH+RTPLV     +D
Sbjct: 10  LFFCSLSLIILTSSATKSQTHVPIRPNKLVLKVQKDRATNLHVVQIHKRTPLVQFPFVID 69

Query: 69  LGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQC-FSPPRPGCNNNTCGLFPG 128
           L G+F+ V+C+  Y SSTYK   C S+QC+ A+ S +C  C  S  RPGC+ N CGL   
Sbjct: 70  LTGRFLSVNCENQYTSSTYKAPVCHSSQCARAN-SHTCRTCSSSKTRPGCHTNACGLLTT 129

Query: 129 NTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCG-STFLLEGLAGGVSGMAGF 188
           N +   S  GE+A DV+ + ST G +P   V+ P+FLF C  S  L +GL   V G+AG 
Sbjct: 130 NPVTQQSAQGELAEDVLKIPSTQGSSPGPMVTYPHFLFACAPSNILQKGLPKNVQGVAGL 189

Query: 189 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 248
           G + ISLP Q A+ F F  KFA+CL+ S    G +F G GPY   P ID+++ LTY P  
Sbjct: 190 GHSPISLPYQLASHFGFPPKFAVCLTSSPGKNGAVFFGEGPYFMKPGIDVSRQLTYAP-- 249

Query: 249 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 308
                    F+ G++  EY+I V+S  IN+  +P       I   G GG  IST  PYT 
Sbjct: 250 ---------FTIGQQ-GEYYINVQSFKINNAMLP------SIPKGGFGGAMISTTTPYTT 309

Query: 309 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQN-KNV 368
           L++ I+ A+ + F  +LR VP V  VAPFGACF A  + ++++GP VPSIDL+L N KN+
Sbjct: 310 LQTPIFRALNQLFMNQLRGVPHVKPVAPFGACFDANRIPTSKMGPTVPSIDLVLDNKKNI 369

Query: 369 IWRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTL 428
           +WRIFGAN+M+Q    V+CL FVDGG+ P+  IVIG  Q+EDNL++FDL  SRLGFSS+L
Sbjct: 370 MWRIFGANAMIQPRPGVMCLAFVDGGMRPKAPIVIGTQQLEDNLLQFDLMNSRLGFSSSL 422

Query: 429 LGRMTTCANFNF 432
           L R T CANFNF
Sbjct: 430 LFRRTNCANFNF 422

BLAST of Lcy10g012340 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 282.7 bits (722), Expect = 6.9e-75
Identity = 172/439 (39.18%), Postives = 245/439 (55.81%), Query Frame = 0

Query: 6   SLCLFS-SILFLVFSTSIAATSFRPKALVLPVTKDPSRQ-YITQIHQRTPLVPVKLTVDL 65
           SLC F+ +ILFL  + +          L+ P++KD + Q Y   +  +TPL P KL + L
Sbjct: 7   SLCNFNLAILFLFLTPTFQI------PLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHL 66

Query: 66  GGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNT--CGLFPG 125
           G    WV CD  Y SS+     C +  C+            S P   C+NN+  C LFP 
Sbjct: 67  GSSLSWVLCDSTYTSSSSHHIPCNTPLCN------------SFPSNACSNNSSLCALFPE 126

Query: 126 NTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGFG 185
           N +   +       D ++L + D    +  V + +F+F C +  LL+GLA    G+A  G
Sbjct: 127 NPVTRNTLLDTALIDSLALPTYDA--SSSLVLISDFIFSCATAHLLQGLAANALGLASLG 186

Query: 186 RTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLFI 245
           R+  SLP+Q + + +  R F +CL  S+   G     +    FL +  +   LTYT L +
Sbjct: 187 RSNYSLPAQISTSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKI--DLTYTQLIV 246

Query: 246 NPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTVL 305
           NPV+   V    + S EYFI + SI IN K + +N+++L +D  G GGTKIST +PYTVL
Sbjct: 247 NPVADTVVTDNPQPSDEYFINLTSIKINGKPLYINSSILTVDQTGFGGTKISTAEPYTVL 306

Query: 306 ESSIYNAVLKTFTTELR--QVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNV 365
           E+SIY   ++ F  E     +    AV PFG C+ A  L+ TR+GP VP++DL++ +++V
Sbjct: 307 ETSIYRLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDV 366

Query: 366 IWRIFGANSMVQV---NDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFS 425
            WRIFG NSMV+V     DV CLGFVDGG   RT IVIG HQ+EDNL++FDL ++R GF+
Sbjct: 367 FWRIFGGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFT 423

Query: 426 STLLGRMTTCANFNFTSKA 436
           STLL +   C+N    + A
Sbjct: 427 STLLLQDAKCSNLKVNNFA 423

BLAST of Lcy10g012340 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.5e-74
Identity = 170/431 (39.44%), Postives = 237/431 (54.99%), Query Frame = 0

Query: 17  VFSTSIAATSFRPKALVLPVTKDPS-RQYITQIHQRTPLVPVKLTVDLGGQFMWVDCDRG 76
           ++  S   +S +P  LVLP+ +D S + +   I +RTPL+ V + +DL G+ +WV C + 
Sbjct: 33  LYHNSQPTSSSKPNLLVLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQH 92

Query: 77  YVSSTYKPARCRSAQCSLASKSSSCGQC--FSPPRPGCNNNTCGLFPGNTIIHLSTSGEV 136
           Y SSTY+   C S QCS A+ +  C  C   +  RPGC+NNTCGL   N +   S  GE+
Sbjct: 93  YSSSTYQAPFCHSTQCSRAN-THQCFTCTDSTTSRPGCHNNTCGLISSNPVTQESGLGEL 152

Query: 137 ASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLE-GLAGGVSGMAGFGRTGISLPSQFA 196
           A DV++L ST G      V +P FLF C  TFL + GL   V G  G G   ISLP+Q  
Sbjct: 153 AQDVLALHSTHGSKLGSLVKIPQFLFSCAPTFLTQKGLPNNVQGALGLGHAPISLPNQLF 212

Query: 197 AAFSFNRKFAICLSGSTRFPGVIFSG-----NGPYHFLPNIDLTKSLTYTPLFINPVSTA 256
           + F   R+F +CLS      G I  G     N   +   ++D+   + YTPL I+     
Sbjct: 213 SHFGLKRQFTMCLSSYPTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTPLTIS----- 272

Query: 257 GVFSAGEKSAEYFIGVKSIVINSKTV---------PLNTTLLKIDSNGVGGTKISTVDPY 316
                  K  EYFI V +I +N   V         P +++    +S+ +GG  I+T +PY
Sbjct: 273 -------KQGEYFIQVSAIRVNKHMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPY 332

Query: 317 TVLESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKN 376
           TVL  SI+    + F   + +  +V AV PFG C+  K +S      GVPS+DLI+   +
Sbjct: 333 TVLRHSIFEVFTQVFANNVPKQAQVKAVGPFGLCYDTKKISG-----GVPSVDLIMDKSD 392

Query: 377 VIWRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGF-SS 429
           V+WRI G N MVQ  D V CLGFVDGGV+ R  I +G HQ+E+NLV FDLA SR+GF ++
Sbjct: 393 VVWRISGENLMVQAQDGVSCLGFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTN 445

BLAST of Lcy10g012340 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.8e-73
Identity = 169/421 (40.14%), Postives = 228/421 (54.16%), Query Frame = 0

Query: 25  TSFRPKALVLPVTKDPSR-QYITQIHQRTPLVPVKLTVDLGGQFMWVDCDRGYVSSTYKP 84
           TS +P  LVLPV +D S   +   IH+RTPL+ V L +DL G+ +WV C + Y SSTY+ 
Sbjct: 40  TSSKPNLLVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSSTYQA 99

Query: 85  ARCRSAQCSLASKSSSCGQC--FSPPRPGCNNNTCGLFPGNTIIHLSTSGEVASDVVSLS 144
             C S QCS A+ +  C  C   +  RPGC+NNTCGL   N +   S  GE+A DV+++ 
Sbjct: 100 PFCHSTQCSRAN-THQCFTCTDSTTTRPGCHNNTCGLLSSNPVTQESGLGELAQDVLAIH 159

Query: 145 STDGFNPTRAVSVPNFLFVCGSTFLLE-GLAGGVSGMAGFGRTGISLPSQFAAAFSFNRK 204
           ST G      V VP FLF C  +FL + GL   V G  G G+  ISL +Q  + F   R+
Sbjct: 160 STHGSKLGPMVKVPQFLFSCAPSFLAQKGLPNNVQGALGLGQAPISLQNQLFSHFGLKRQ 219

Query: 205 FAICLSGSTRFPGVIFSG-----NGPYHFLPNIDLTKSLTYTPLFINPVSTAGVFSAGEK 264
           F++CLS  +   G I  G     N   +   ++D+   L YTPL I+            K
Sbjct: 220 FSVCLSRYSTSNGAILFGDINDPNNNNYIHNSLDVLHDLVYTPLTIS------------K 279

Query: 265 SAEYFIGVKSIVINSK-TVPLNTTLLKIDSNG------VGGTKISTVDPYTVLESSIYNA 324
             EYFI V +I +N    +P     +   S        +GG  I+T  PYTVL  SI+  
Sbjct: 280 QGEYFIQVNAIRVNKHLVIPTKNPFISPSSTSYHGSGEIGGALITTTHPYTVLSHSIFEV 339

Query: 325 VLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVIWRIFGANS 384
             + F   + +  +V AV PFG C+ ++ +S      G PS+DLIL   + +WRI   N 
Sbjct: 340 FTQVFANNMPKQAQVKAVGPFGLCYDSRKISG-----GAPSVDLILDKNDAVWRISSENF 399

Query: 385 MVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGF-SSTLLGRMTTCA 429
           MVQ  D V CLGFVDGGV+ R  I +GAH +E+NLV FDL  SR+GF S++L     TC+
Sbjct: 400 MVQAQDGVSCLGFVDGGVHARAGIALGAHHLEENLVVFDLERSRVGFNSNSLKSYGKTCS 442

BLAST of Lcy10g012340 vs. ExPASy TrEMBL
Match: A0A6J1F9F8 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111443306 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.8e-225
Identity = 394/434 (90.78%), Postives = 416/434 (95.85%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MATS SL  FSS+LFL+ S++IAATSFRPKALVLPVTK PS QYITQI QRTPLVPVKLT
Sbjct: 1   MATSISLSFFSSVLFLLLSSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLG QFMWVDCDRGY+SSTYKPARCRSAQC+LASKSS CGQCFSPPRPGCNNNTC LFP
Sbjct: 61  VDLGSQFMWVDCDRGYISSTYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGE+ASDVVS+SSTDGFNPT+ V+VPNFLFVCGSTFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGELASDVVSVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GR GISLPSQF+AAFSFNRKFA+CLSGSTRFPGVIFSGNGPYHFLPNIDLT SLTYTPLF
Sbjct: 181 GRNGISLPSQFSAAFSFNRKFAVCLSGSTRFPGVIFSGNGPYHFLPNIDLTDSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVF+AGEKS EYFIGVKSIVINSKTVPLNTTLLKIDSNG+GGTKISTVDPYTV
Sbjct: 241 INPVSTAGVFTAGEKSTEYFIGVKSIVINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAVLKTFTTEL+ VPRVAAVAPFGACF AKS+SSTRLGPGVPSI+LILQNK VI
Sbjct: 301 LESSIYNAVLKTFTTELKNVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMVQV DDVLCLGFVDGGVNPRTSIVIGAHQIEDNL+EFD+ATSRLGFS+TLL
Sbjct: 361 WRIFGANSMVQVKDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTCANFNFTSK
Sbjct: 421 GRMTTCANFNFTSK 434

BLAST of Lcy10g012340 vs. ExPASy TrEMBL
Match: A0A6J1IFL5 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111472330 PE=4 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 3.9e-222
Identity = 390/434 (89.86%), Postives = 412/434 (94.93%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MATSTSL  F S+LFL+ S++IAATSFRPKALVLPVTK PS QYITQI QRTPLVPVKLT
Sbjct: 1   MATSTSLSFFYSVLFLLLSSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLG QFMWVDCDRGY+SSTYKPARCRSAQC+LASKSS CGQCFSPPRPGCNNNTC LFP
Sbjct: 61  VDLGSQFMWVDCDRGYISSTYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGE+ASDVVS+SSTDGFNPT+ V+VPNFLFVCG+TFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGELASDVVSVSSTDGFNPTKPVTVPNFLFVCGTTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GR GISLPSQF+AAFSFNRKFAICLSGST FPGVIFSGNGPYHFLPNIDLT SLTYTPLF
Sbjct: 181 GRNGISLPSQFSAAFSFNRKFAICLSGSTSFPGVIFSGNGPYHFLPNIDLTDSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVF+ GEKS EYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV
Sbjct: 241 INPVSTAGVFTTGEKSTEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAVLKTFTT L+ +PRVAAVAPFGACF AKS+SSTRLGPGVPSI+LILQNK VI
Sbjct: 301 LESSIYNAVLKTFTTALKNIPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMV V DDVLCLGFVDGGVNPRTSIVIGAHQIE+NL+EFDLATSRLGFS+TLL
Sbjct: 361 WRIFGANSMVHVKDDVLCLGFVDGGVNPRTSIVIGAHQIENNLLEFDLATSRLGFSATLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTCANFNFTSK
Sbjct: 421 GRMTTCANFNFTSK 434

BLAST of Lcy10g012340 vs. ExPASy TrEMBL
Match: A0A6J1IGY1 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111475005 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 8.7e-222
Identity = 389/434 (89.63%), Postives = 412/434 (94.93%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MATSTSL  F S+LFL+ S++IAATSFRPKALVLPVTK PS QYITQI QRTPLVP+KLT
Sbjct: 1   MATSTSLSFFYSVLFLLLSSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPLKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLG QFMWVDCDRGY+SSTYKPARCRSAQC+LASKSS CGQCFSPPRPGCNNNTC LFP
Sbjct: 61  VDLGSQFMWVDCDRGYISSTYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGE+ASDVVS+SSTDGFNPT+ V+VPNFLFVCG+TFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGELASDVVSVSSTDGFNPTKPVTVPNFLFVCGTTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GR GISLPSQF+AAFSFNRKFAICLSGST FPGVIFSGNGPYHFLPNIDLT SLTYTPLF
Sbjct: 181 GRNGISLPSQFSAAFSFNRKFAICLSGSTSFPGVIFSGNGPYHFLPNIDLTDSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVF+ GEKS EYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV
Sbjct: 241 INPVSTAGVFTTGEKSTEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAVLKTFTT L+ +PRVAAVAPFGACF AKS+SSTRLGPGVPSI+LILQNK VI
Sbjct: 301 LESSIYNAVLKTFTTALKNIPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMV V DDVLCLGFVDGGVNPRTSIVIGAHQIE+NL+EFDLATSRLGFS+TLL
Sbjct: 361 WRIFGANSMVHVKDDVLCLGFVDGGVNPRTSIVIGAHQIENNLLEFDLATSRLGFSATLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTCANFNFTSK
Sbjct: 421 GRMTTCANFNFTSK 434

BLAST of Lcy10g012340 vs. ExPASy TrEMBL
Match: A0A6J1IWG3 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111479193 PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.3e-214
Identity = 379/434 (87.33%), Postives = 406/434 (93.55%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           M  STSL  FS ILFL+ ST+IAATSFRPKAL+LPVTKDPS Q+I Q+ QRTPLVPVKLT
Sbjct: 1   MGASTSLSFFSFILFLLLSTAIAATSFRPKALLLPVTKDPSLQHIIQLRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLGGQFMWVDC+R Y SSTYKPARCRS+QCSLASKS+SCGQCFSPPRPGCNNNTCGLFP
Sbjct: 61  VDLGGQFMWVDCNRAYFSSTYKPARCRSSQCSLASKSTSCGQCFSPPRPGCNNNTCGLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGEVASDVVS+SST+GFNPT  VSVPNFLFVCG+TFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGEVASDVVSVSSTNGFNPTSPVSVPNFLFVCGTTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GRTGISLPSQFAAAFSFNRKFAICLS ST+FPGVIFSGNGPY+FLPNIDLTKSLTYTPLF
Sbjct: 181 GRTGISLPSQFAAAFSFNRKFAICLSSSTKFPGVIFSGNGPYNFLPNIDLTKSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKI+S G+GGTKISTV+PYTV
Sbjct: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKINSKGIGGTKISTVNPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIY AVLKT TTEL ++PRV AVAPF ACF A +  STRLGPG+PSIDLILQNKNVI
Sbjct: 301 LESSIYKAVLKTLTTELGKIPRVPAVAPFEACFNANNFGSTRLGPGMPSIDLILQNKNVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMVQ+N+ VLCLGFVDGGV PRTSIVIGAHQIED L+EFDLATSRLGF STLL
Sbjct: 361 WRIFGANSMVQMNEKVLCLGFVDGGVEPRTSIVIGAHQIEDTLLEFDLATSRLGFISTLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTC+NFNFT+K
Sbjct: 421 GRMTTCSNFNFTAK 434

BLAST of Lcy10g012340 vs. ExPASy TrEMBL
Match: A0A6J1GV86 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111457731 PE=4 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 5.6e-213
Identity = 377/434 (86.87%), Postives = 404/434 (93.09%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MA STSL  FSSILFL+ S++IAATSFRPKAL+LPVTKDPS Q+I Q+ QRTPLVPVKLT
Sbjct: 1   MAASTSLSFFSSILFLLLSSAIAATSFRPKALLLPVTKDPSLQHIIQLRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLGGQFMWVDC+R Y+SSTYKPARCRSAQCSLASKS+SCGQCFSPPRPGCNNNTCGLFP
Sbjct: 61  VDLGGQFMWVDCNRAYISSTYKPARCRSAQCSLASKSTSCGQCFSPPRPGCNNNTCGLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GN IIHLSTSGEVASDVVS+SST+GFNPT  VSVP FLFVCG+TFLL+GLAGGV+GMAGF
Sbjct: 121 GNNIIHLSTSGEVASDVVSVSSTNGFNPTTPVSVPKFLFVCGTTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GRTGISLPSQFAAAFS NRKFAICLS STR PGVIFSGNGPY+FLPNIDLTKS TYTPLF
Sbjct: 181 GRTGISLPSQFAAAFSLNRKFAICLSSSTRLPGVIFSGNGPYNFLPNIDLTKSFTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGV SAGEKSAEYFIGVKSIVINSKTVPLNTTLLKI+S G+GGTKISTV+PYTV
Sbjct: 241 INPVSTAGVLSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKINSKGIGGTKISTVNPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIY AVLKTFTTELR++PRV AVAPF  CF A S  STRLGPG+PSIDLILQNKNVI
Sbjct: 301 LESSIYKAVLKTFTTELRKIPRVPAVAPFEVCFNANSFGSTRLGPGMPSIDLILQNKNVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMVQVN+ VLCLGFV+GGV PRTSIVIGAHQI+D L+EFDLATSRLGF STLL
Sbjct: 361 WRIFGANSMVQVNEKVLCLGFVNGGVEPRTSIVIGAHQIQDALLEFDLATSRLGFISTLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTC+NFNFT+K
Sbjct: 421 GRMTTCSNFNFTAK 434

BLAST of Lcy10g012340 vs. NCBI nr
Match: XP_038896289.1 (probable aspartic proteinase GIP2 [Benincasa hispida])

HSP 1 Score: 796.6 bits (2056), Expect = 1.1e-226
Identity = 403/435 (92.64%), Postives = 417/435 (95.86%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MA STSL  FSSILFL+FS SIA TSFRPK+LVLPVTK PS QYITQIHQRTPLVPVKLT
Sbjct: 1   MAASTSLAFFSSILFLLFSISIAKTSFRPKSLVLPVTKHPSLQYITQIHQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP
Sbjct: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTII LSTSGEVASDVVS+SSTDGFNPTRAVS+PNFLFVCGSTFLLEGLAGGV+GMAGF
Sbjct: 121 GNTIIRLSTSGEVASDVVSVSSTDGFNPTRAVSLPNFLFVCGSTFLLEGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GRTGISLPSQFAAAFSFNRKFA+CLSGSTRFPGVIFSGNGPYHFLPN DLTKSLTYTPLF
Sbjct: 181 GRTGISLPSQFAAAFSFNRKFAVCLSGSTRFPGVIFSGNGPYHFLPNTDLTKSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNG GGTKISTV+PYTV
Sbjct: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGNGGTKISTVNPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAV+KTFTTEL +VPRVAAVAPFG C+ +KS SSTRLGPGVPSIDLILQNK VI
Sbjct: 301 LESSIYNAVVKTFTTELSKVPRVAAVAPFGVCYNSKSFSSTRLGPGVPSIDLILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMV VND+VLCLGFVDGGV PRT+IVIGAHQIEDNL+EFDLATSRLGFSSTLL
Sbjct: 361 WRIFGANSMVFVNDNVLCLGFVDGGVEPRTAIVIGAHQIEDNLLEFDLATSRLGFSSTLL 420

Query: 421 GRMTTCANFNFTSKA 436
           GRMTTCANFNFTS A
Sbjct: 421 GRMTTCANFNFTSNA 435

BLAST of Lcy10g012340 vs. NCBI nr
Match: KAG7028922.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 793.5 bits (2048), Expect = 9.2e-226
Identity = 396/434 (91.24%), Postives = 417/434 (96.08%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MATSTSL  FSS+LFL+ S++IAATSFRPKALVLPVTK PS QYITQI QRTPLVPVKLT
Sbjct: 1   MATSTSLSFFSSVLFLLLSSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLG QFMWVDCDRGY+SSTYKPARCRSAQC+LASKSS CGQCFSPPRPGCNNNTC LFP
Sbjct: 61  VDLGSQFMWVDCDRGYISSTYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGE+ASDVVS+SSTDGFNPT+ V+VPNFLFVCGSTFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGELASDVVSVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GR GISLPSQF+AAFSFNRKFA+CLSGSTRFPGVIF+GNGPYHFLPNIDLT SLTYTPLF
Sbjct: 181 GRNGISLPSQFSAAFSFNRKFAVCLSGSTRFPGVIFAGNGPYHFLPNIDLTDSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVFSAGEKS EYFIGVKSIVINSKTVPLNTTLLKIDSNG+GGTKISTVDPYTV
Sbjct: 241 INPVSTAGVFSAGEKSTEYFIGVKSIVINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAVLKTFTTEL+ VPRVAAVAPFGACF AKS+SSTRLGPGVPSI+LILQNK VI
Sbjct: 301 LESSIYNAVLKTFTTELKNVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMVQV DDVLCLGFVDGGVNPRTSIVIGAHQIEDNL+EFDLATSRLGFS+TLL
Sbjct: 361 WRIFGANSMVQVKDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDLATSRLGFSATLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTCANFNFTSK
Sbjct: 421 GRMTTCANFNFTSK 434

BLAST of Lcy10g012340 vs. NCBI nr
Match: KAG6597463.1 (putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.6 bits (2043), Expect = 3.5e-225
Identity = 394/434 (90.78%), Postives = 417/434 (96.08%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MATSTSL  FSS+LFL+ S++IAATSFRPKALVLPVTK PS QYITQI QRTPLVPVKLT
Sbjct: 1   MATSTSLSFFSSVLFLLLSSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLG QFMWVDCDRGY+SSTYKPARCRSAQC+LASKSS CGQCFSPPRPGCNNNTC LFP
Sbjct: 61  VDLGSQFMWVDCDRGYISSTYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGE+ASDVVS+SSTDGFNPT+ V+VPNFLFVCGSTFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGELASDVVSVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GR GISLPSQF+AAFSFNRKFA+CLSGSTRFPGVIF+GNGPYHFLPNIDLT SLTYTPLF
Sbjct: 181 GRNGISLPSQFSAAFSFNRKFAVCLSGSTRFPGVIFAGNGPYHFLPNIDLTDSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVFSAGEKS EYFIGVKSI+INSKTVPLNTTLLKIDSNG+GGTKISTVDPYTV
Sbjct: 241 INPVSTAGVFSAGEKSTEYFIGVKSILINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAVLKTFTTEL+ VPRVAAVAPFGACF AKS+SSTRLGPGVPSI+LILQNK VI
Sbjct: 301 LESSIYNAVLKTFTTELKNVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMVQV DDVLCLGFVDGGVNPRTSIVIGAHQIEDNL+EFD+ATSRLGFS+TLL
Sbjct: 361 WRIFGANSMVQVKDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTCANFNFTSK
Sbjct: 421 GRMTTCANFNFTSK 434

BLAST of Lcy10g012340 vs. NCBI nr
Match: XP_022936844.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 790.4 bits (2040), Expect = 7.8e-225
Identity = 394/434 (90.78%), Postives = 416/434 (95.85%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MATS SL  FSS+LFL+ S++IAATSFRPKALVLPVTK PS QYITQI QRTPLVPVKLT
Sbjct: 1   MATSISLSFFSSVLFLLLSSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLG QFMWVDCDRGY+SSTYKPARCRSAQC+LASKSS CGQCFSPPRPGCNNNTC LFP
Sbjct: 61  VDLGSQFMWVDCDRGYISSTYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGE+ASDVVS+SSTDGFNPT+ V+VPNFLFVCGSTFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGELASDVVSVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GR GISLPSQF+AAFSFNRKFA+CLSGSTRFPGVIFSGNGPYHFLPNIDLT SLTYTPLF
Sbjct: 181 GRNGISLPSQFSAAFSFNRKFAVCLSGSTRFPGVIFSGNGPYHFLPNIDLTDSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVF+AGEKS EYFIGVKSIVINSKTVPLNTTLLKIDSNG+GGTKISTVDPYTV
Sbjct: 241 INPVSTAGVFTAGEKSTEYFIGVKSIVINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAVLKTFTTEL+ VPRVAAVAPFGACF AKS+SSTRLGPGVPSI+LILQNK VI
Sbjct: 301 LESSIYNAVLKTFTTELKNVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMVQV DDVLCLGFVDGGVNPRTSIVIGAHQIEDNL+EFD+ATSRLGFS+TLL
Sbjct: 361 WRIFGANSMVQVKDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTCANFNFTSK
Sbjct: 421 GRMTTCANFNFTSK 434

BLAST of Lcy10g012340 vs. NCBI nr
Match: XP_023538677.1 (basic 7S globulin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.9 bits (2036), Expect = 2.3e-224
Identity = 395/434 (91.01%), Postives = 416/434 (95.85%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MATSTSL  FSS+LFL+FS ++A TSFRPKALVLPVTK PS QYITQI QRTPLVPVKLT
Sbjct: 1   MATSTSLSFFSSVLFLLFS-AVATTSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLT 60

Query: 61  VDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFP 120
           VDLG QFMWVDCDRGY+SSTYKPARCRSAQC+LASKSS CGQCFSPPRPGCNNNTC LFP
Sbjct: 61  VDLGSQFMWVDCDRGYISSTYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFP 120

Query: 121 GNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGF 180
           GNTIIHLSTSGE+ASDVVS+SSTDGFNPT+ V+VPNFLFVCGSTFLL+GLAGGV+GMAGF
Sbjct: 121 GNTIIHLSTSGELASDVVSVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGF 180

Query: 181 GRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLF 240
           GR GISLPSQF+AAFSFNRKFA+CLSGSTRFPGVIFSGNGPYHFLPNIDLT SLTYTPLF
Sbjct: 181 GRNGISLPSQFSAAFSFNRKFAVCLSGSTRFPGVIFSGNGPYHFLPNIDLTDSLTYTPLF 240

Query: 241 INPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300
           INPVSTAGVFSAGEKS EYFIGVKSI+INSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV
Sbjct: 241 INPVSTAGVFSAGEKSTEYFIGVKSILINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTV 300

Query: 301 LESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVI 360
           LESSIYNAVLKTFTTEL+ VPRVAAVAPFGACF AKS+SSTRLGPGVPSI+LILQNK VI
Sbjct: 301 LESSIYNAVLKTFTTELKNVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVI 360

Query: 361 WRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLL 420
           WRIFGANSMVQV DDVLCLGFVDGGVNPRTSIVIGAHQIEDNL+EFD+ATSRLGFS+TLL
Sbjct: 361 WRIFGANSMVQVKDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLL 420

Query: 421 GRMTTCANFNFTSK 435
           GRMTTCANFNFTSK
Sbjct: 421 GRMTTCANFNFTSK 433

BLAST of Lcy10g012340 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 535.0 bits (1377), Expect = 5.5e-152
Identity = 282/432 (65.28%), Postives = 332/432 (76.85%), Query Frame = 0

Query: 9   LFSSILFLVFS-TSIAATSFRPKALVLPVTKDPSR-QYITQIHQRTPLVPVKLTVDLGGQ 68
           +FS +L  +FS +S A T FRPKAL+LPVTKD S  QY T I+QRTPLVP  +  DLGG+
Sbjct: 7   IFSVLLLFIFSLSSSAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGR 66

Query: 69  FMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFPGNTIIH 128
            +WVDCD+GYVSSTY+  RC SA CS A  S+SCG CFSPPRPGC+NNTCG  P NT+  
Sbjct: 67  ELWVDCDKGYVSSTYQSPRCNSAVCSRAG-STSCGTCFSPPRPGCSNNTCGGIPDNTVTG 126

Query: 129 LSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGFGRTGIS 188
            +TSGE A DVVS+ ST+G NP R V +PN +F CG+TFLL+GLA G  GMAG GR  I 
Sbjct: 127 TATSGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTVGMAGMGRHNIG 186

Query: 189 LPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLFINPVST 248
           LPSQFAAAFSF+RKFA+CL   T   GV F GNGPY FLP I ++ SL  TPL INPVST
Sbjct: 187 LPSQFAAAFSFHRKFAVCL---TSGKGVAFFGNGPYVFLPGIQIS-SLQTTPLLINPVST 246

Query: 249 AGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKID-SNGVGGTKISTVDPYTVLESSI 308
           A  FS GEKS+EYFIGV +I I  KTVP+N TLLKI+ S G+GGTKIS+V+PYTVLESSI
Sbjct: 247 ASAFSQGEKSSEYFIGVTAIQIVEKTVPINPTLLKINASTGIGGTKISSVNPYTVLESSI 306

Query: 309 YNAVLKTFTTE--LRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNVIWRI 368
           YNA    F  +   R + RVA+V PFGACF  K++  TRLG  VP I+L+L +K+V+WRI
Sbjct: 307 YNAFTSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIELVLHSKDVVWRI 366

Query: 369 FGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLLGRM 428
           FGANSMV V+DDV+CLGFVDGGVN RTS+VIG  Q+EDNL+EFDLA+++ GFSSTLLGR 
Sbjct: 367 FGANSMVSVSDDVICLGFVDGGVNARTSVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQ 426

Query: 429 TTCANFNFTSKA 436
           T CANFNFTS A
Sbjct: 427 TNCANFNFTSTA 433

BLAST of Lcy10g012340 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 515.4 bits (1326), Expect = 4.5e-146
Identity = 274/437 (62.70%), Postives = 327/437 (74.83%), Query Frame = 0

Query: 4   STSLCLFSSILFLVFS-TSIAATSFRPKALVLPVTKDPSR-QYITQIHQRTPLVPVKLTV 63
           S+ + +FS +L  +FS +S A  SFRPKAL+LPVTKDPS  QY T I+QRTPLVP  +  
Sbjct: 3   SSRIIIFSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVF 62

Query: 64  DLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFPG 123
           DLGG+  WVDCD+GYVS+TY+  RC SA CS A  S +CG CFSPPRPGC+NNTCG FP 
Sbjct: 63  DLGGREFWVDCDQGYVSTTYRSPRCNSAVCSRAG-SIACGTCFSPPRPGCSNNTCGAFPD 122

Query: 124 NTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGFG 183
           N+I   +TSGE A DVVS+ ST+G NP R V +PN +F CGST LL+GLA G  GMAG G
Sbjct: 123 NSITGWATSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKGAVGMAGMG 182

Query: 184 RTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLFI 243
           R  I LP QFAAAFSFNRKFA+CL+      GV F GNGPY FLP I +++ L  TPL I
Sbjct: 183 RHNIGLPLQFAAAFSFNRKFAVCLTSGR---GVAFFGNGPYVFLPGIQISR-LQKTPLLI 242

Query: 244 NPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKID-SNGVGGTKISTVDPYTV 303
           NP +T   FS GEKS EYFIGV +I I  KT+P++ TLLKI+ S G+GGTKIS+V+PYTV
Sbjct: 243 NPGTTVFEFSKGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTGIGGTKISSVNPYTV 302

Query: 304 LESSIYNAVLKTFTTE--LRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKN 363
           LESSIY A    F  +   R + RVA+V PFGACF  K++  TRLG  VP I L+L +K+
Sbjct: 303 LESSIYKAFTSEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIQLVLHSKD 362

Query: 364 VIWRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSST 423
           V+WRIFGANSMV V+DDV+CLGFVDGGVNP  S+VIG  Q+EDNL+EFDLA+++ GFSST
Sbjct: 363 VVWRIFGANSMVSVSDDVICLGFVDGGVNPGASVVIGGFQLEDNLIEFDLASNKFGFSST 422

Query: 424 LLGRMTTCANFNFTSKA 436
           LLGR T CANFNFTS A
Sbjct: 423 LLGRQTNCANFNFTSTA 434

BLAST of Lcy10g012340 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 195.7 bits (496), Expect = 7.9e-50
Identity = 150/432 (34.72%), Postives = 212/432 (49.07%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKDPSRQYITQIHQRTPLVPVKLT 60
           MA      L S +   + +TS +   F  ++ + P+ KD ++   T           K  
Sbjct: 1   MAPRVIFLLLSLVFLYLANTSHSLRKF--QSFLHPIYKDTAKNIYTIPLSIGSTSSEKFV 60

Query: 61  VDLGGQF-MWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLF 120
           +DL G   +  +C     S+TY P RC S +C  A+           P   C NN   + 
Sbjct: 61  LDLNGAAPLLQNCPTAAKSTTYHPIRCGSTRCKYAN-----------PNFPCPNNV--IA 120

Query: 121 PGNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAG 180
              T+   S +  +  D V L  T     TR   + + L    +    +G         G
Sbjct: 121 KKRTVCLSSDNSRLFRDTVPLLYTFNGVYTRDSEMSSSL----TLTCTDGAPALKQRTIG 180

Query: 181 FGRTGISLPSQFAAAFSFNRKFAICLSGSTR---FPGVIFSGNGPYHFLP-NIDLTKSLT 240
              T +S+PSQ  + +    K A+CL  + R     G ++ G G Y++LP + D++K   
Sbjct: 181 LANTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIGKGEYYYLPYDKDVSKIFA 240

Query: 241 YTPLFINPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTV 300
            TPL  N            KS EY I VKSI I +KTVP+            G TKIST+
Sbjct: 241 STPLIGN-----------GKSGEYLIDVKSIQIGAKTVPI----------PYGATKISTL 300

Query: 301 DPYTVLESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQ 360
            PYTV ++S+Y A+L  FT  ++ + +  AV PFGACF +        G GVP IDL+L 
Sbjct: 301 APYTVFQTSLYKALLTAFTENIK-IAKAPAVKPFGACFYSNG------GRGVPVIDLVLS 360

Query: 361 NKNVIWRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGF 420
                WRI+G+NS+V+VN +V+CLGFVDGGV P+  IVIG  Q+EDNLVEFDL  S+  F
Sbjct: 361 G-GAKWRIYGSNSLVKVNKNVVCLGFVDGGVKPKYPIVIGGFQMEDNLVEFDLEASKFSF 384

Query: 421 SSTLLGRMTTCA 428
           SS+LL   T+C+
Sbjct: 421 SSSLLLHNTSCS 384

BLAST of Lcy10g012340 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 186.8 bits (473), Expect = 3.7e-47
Identity = 136/423 (32.15%), Postives = 213/423 (50.35%), Query Frame = 0

Query: 16  LVFSTSIAATSFRPKA-LVLPVTK-DPSRQYITQIHQRTPL-VPVKLTVDLGGQFMWVDC 75
           LVF +  AA + +  +  +LP+TK +P+  + T  +  +    PV L +DLG    W+DC
Sbjct: 9   LVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNLTWLDC 68

Query: 76  DRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFPGNTI-IHLSTSG 135
            +    S+ +   C+S+ C             S P  GC   +C     N +  +   +G
Sbjct: 69  RKLKSLSSLRLVTCQSSTCK------------SIPGNGCAGKSCLYKQPNPLGQNPVVTG 128

Query: 136 EVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAGFGRTGISLPSQF 195
            V  D  SL +TDG      VSV +F F C     L+GL   V G+        S   Q 
Sbjct: 129 RVVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLALSPGSSSFTKQV 188

Query: 196 AAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPLFINPVSTAGVFS 255
            +AF+   KF++CL  S       F   G ++F+P  + +          NP+       
Sbjct: 189 TSAFNVIPKFSLCLPSSGTGH---FYIAGIHYFIPPFNSSD---------NPIPRTLTPI 248

Query: 256 AGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTVLESSIYNAVLK 315
            G  S +Y I VKSI +    + LN  LL       GG K+STV  YTVL++ IYNA+ +
Sbjct: 249 KGTDSGDYLITVKSIYVGGTALKLNPDLL------TGGAKLSTVVHYTVLQTDIYNALAQ 308

Query: 316 TFTTELRQ--VPRVAAVAPFGACFKAKSL-SSTRLGPGVPSIDLILQNK--NVIWRIFGA 375
           +FT + +   + +V +VAPF  CF +++   +   GP VP I++ L  +   V W  +GA
Sbjct: 309 SFTLKAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPNVPVIEIGLPGRIGEVKWGFYGA 368

Query: 376 NSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGFSSTLLGRMTTC 430
           N++V+V + V+CL F+DGG  P+  +VIG HQ++D+++EFD + + L FS +LL   T+C
Sbjct: 369 NTVVKVKETVMCLAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGTVLAFSESLLLHNTSC 401

BLAST of Lcy10g012340 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-46
Identity = 143/416 (34.38%), Postives = 193/416 (46.39%), Query Frame = 0

Query: 1   MATSTSLCLFSSILFLVFSTSIAATSFRPKALVLPVTKD-PSRQYITQIHQRTPLVPVKL 60
           MA+S+ L LF          S +  S     +V PV KD P+ QY+ QI       PVKL
Sbjct: 1   MASSSCLNLFFFSFLSALIISKSQISDSVNGVVFPVVKDLPTGQYLAQIRLGDSPDPVKL 60

Query: 61  TVDLGGQFMWVDCDRGYVSSTYKPARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLF 120
            VDL G  +W DC   +VSS+       S+ C  A   +      S  R    N  C L 
Sbjct: 61  VVDLAGSILWFDCSSRHVSSSRNLISGSSSGCLKAKVGNERVSSSSSSRKD-QNADCELL 120

Query: 121 PGNTIIHLSTSGEVASDVVSLSSTDGFNPTRAVSVPNFLFVCGSTFLLEGLAGGVSGMAG 180
             N    ++  GE+ SDV+S+ S        +    + LF C   +LL GLA G  G+ G
Sbjct: 121 VKNDAFGITARGELFSDVMSVGS------VTSPGTVDLLFACTPPWLLRGLASGAQGVMG 180

Query: 181 FGRTGISLPSQFAAAFSFNRKFAICLSGSTRFPGVIFSGNGPYHFLPNIDLTKSLTYTPL 240
            GR  ISLPSQ AA  +  R+  + LS      GV+ + +    F   +  ++SL YTPL
Sbjct: 181 LGRAQISLPSQLAAETNERRRLTVYLS---PLNGVVSTSSVEEVF--GVAASRSLVYTPL 240

Query: 241 FINPVSTAGVFSAGEKSAEYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYT 300
                           S  Y I VKSI +N +         K+   G    ++STV PYT
Sbjct: 241 LTG------------SSGNYVINVKSIRVNGE---------KLSVEGPLAVELSTVVPYT 300

Query: 301 VLESSIYNAVLKTFTTELRQVPRVAAVAPFGACFKAKSLSSTRLGPGVPSIDLILQNKNV 360
           +LESSIY    + +     +   V  VAPFG CF +            P++DL LQ++ V
Sbjct: 301 ILESSIYKVFAEAYAKAAGEATSVPPVAPFGLCFTS--------DVDFPAVDLALQSEMV 360

Query: 361 IWRIFGANSMVQVNDDVLCLGFVDGGVNPRTSIVIGAHQIEDNLVEFDLATSRLGF 416
            WRI G N MV V   V C G VDGG +    IV+G  Q+E  +++FDL  S +GF
Sbjct: 361 RWRIHGKNLMVDVGGGVRCSGIVDGGSSRVNPIVMGGLQLEGFILDFDLGNSMMGF 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0DO215.7e-17069.02Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
P829529.9e-9846.99Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
I1JNS66.9e-7539.18Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
Q9FSH91.5e-7439.44Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1[more]
Q423693.8e-7340.14Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F9F83.8e-22590.78basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111443306 PE=4 SV=1[more]
A0A6J1IFL53.9e-22289.86basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111472330 PE=4 SV=1[more]
A0A6J1IGY18.7e-22289.63basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111475005 PE=4 SV=1[more]
A0A6J1IWG32.3e-21487.33basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111479193 PE=4 SV=1[more]
A0A6J1GV865.6e-21386.87basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111457731 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038896289.11.1e-22692.64probable aspartic proteinase GIP2 [Benincasa hispida][more]
KAG7028922.19.2e-22691.24Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6597463.13.5e-22590.78putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022936844.17.8e-22590.78basic 7S globulin-like [Cucurbita moschata][more]
XP_023538677.12.3e-22491.01basic 7S globulin-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G03220.15.5e-15265.28Eukaryotic aspartyl protease family protein [more]
AT1G03230.14.5e-14662.70Eukaryotic aspartyl protease family protein [more]
AT5G19100.17.9e-5034.72Eukaryotic aspartyl protease family protein [more]
AT5G19110.13.7e-4732.15Eukaryotic aspartyl protease family protein [more]
AT5G19120.11.4e-4634.38Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 24..219
e-value: 8.5E-54
score: 184.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 220..433
e-value: 1.3E-71
score: 242.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 39..426
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 258..416
e-value: 1.1E-57
score: 194.3
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 44..219
e-value: 2.1E-42
score: 145.3
NoneNo IPR availablePANTHERPTHR47965:SF22EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 10..435
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 10..435
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 44..416
score: 18.820734
IPR033868Xylanase inhibitor I-likeCDDcd05489xylanase_inhibitor_I_likecoord: 49..419
e-value: 2.99442E-151
score: 431.775

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g012340.1Lcy10g012340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity