Lcy10g010340 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy10g010340
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	diacylglycerol O-acyltransferase 3, cytosolic-like
Location	Chr10: 38137486 .. 38139351 (+)
RNA-Seq Expression	Lcy10g010340
Synteny	Lcy10g010340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGACTTCAAATCTTCAATCAATCAATTCTTTCGAATCAATTTTCCCCAAATCCATGGAGGTCTCCGGCGCCGTATCCCGGCGGCTCCCCGGCCTCTTCCGCCGTGAGATTGCTCAGTCGTCTATGGAAGTCTCCGGCGCCGATGCTCGGCGGGCCCCCTGTAGCCTGTCCGTTTCCGGTGGCTCTGTTGGTATTGGAACTCGTGATCTTGGCCTCGTCGGTCGTCGGAATGATGATTCTTCTAGGTTTTGCGATGACGGTCATTTAAGGTATTATGTTGGACCGAGATGCCATGGGGGAAGATTGAAGAAGGAGAAGGAAGCGGTGAAGAAGAAATTGAAGTTGTTGAAAGGATTGTCCGGTGGTTTATCTGCGGCTTATGAATCGGGCTTCGTGTTTCGTTTTGATGATGGCTCGGTCGGTGAATTTCAAGGGGATCGCTTCTCTGTAAGTTGAACGATTCTGGAATCTATCTTTATTTCTCTCTATCGTTGTTAAAATCTATTGATTCGAATCCAACTTGCTCGAGCCACTTGATCTGTAAATACTGAATGATTATGATGTTTTCCCCTTGAATTTATCTGAATTAAACTATATTAATCATATCATGTTGAATTGGAGTTGATTTAAGATTTGTTACATTTATTTGCCATCGAATTCTTCTGTTTTTGCTTTGATTGTCGAGAGAGCCATTGAATGATTTCTTGGTGTGTTTGTTTTGGCTGCATGGATTGGTTGATAACATCCATTGATCGGATTGTTGTTCCCTTGATTGCAGATCGAGGAAACAAGAGAGGCATTGGGAAGACAACTCCAACAGCTAAAAGCAGAGGAGAAAGAACAAAAAAGAAAGAGGAAGCTAGAGAAGGCCAAGCTGAAAGCAGCTCGAGCACAAACCATTCACGAGTCATCATCTTCATCCTCCGAATCCAGTGACAATGAAAGTAATGCAATCAACACAAGCTACCGAATAAGGAAGGCGCTTTCACAGCCATTCCCAGATCAATGGCAGACAAAAGCCATTCAAGGATCAGCACTGCCTCCTCCTTTGCAAACCCTGCAGCTTGGTTCAAACACCGAGAACACCGGTGTCGCCCGGCCGGCATCTGTAGGGAGGATTGAAGTGTGCATGGGGAACAAGTGCAAGAAGGCAGGGGCAGCAGCATTGATGGAGGAATTTGAGAGGGTGGTGGGTGCTCATGGAGTTGTTTGTGGGTGTAAATGCATGGGAAAATGCAGAGATGGACCAAATGTGAGAGTTTCTGGTTCTTTGGACAAGAAGCTCGCTCTGTAAATCCTCTGTGCATTGGAGTTGGAGTGGAGGATGTTGGTAGAATTGTAGCTGATTATCTAGGACAAGAAGGGCAGAAACAATCTAGGTTTGCACCTGCAATTTGATGCTTCATTCATTAGACGAGTTAACTTGTGCAGTTTGATTGAGTTTGTAAAAAGTTTAGGATAAGTTACAAATTTGATTGGAGAAAAGTTAGAATTAATTCTAACTATGAAAATCATATGAACTAAATTCTAACTTTCTCCAAACTATTAGGGATCAAATTTGTAATTTAACCAAAAGTTTTTCATCTTTCCTTTTGGTCACACTGAGTTTAAAAGGTTGGATTATACTGATTTGATAGTCGCAAGGAAATTTTTGGACCCAAACCAACTATTAGGATTGTAAATTTGTCGATTTCAATTGTAGGTAGGTGTTGAATCCAAAGTCAATTCAGGTTATTTCTTCATTTGTTCATTCTTGTTCAAAAAATAAAGAGGGTGTGAGTCTTTTGAATATTTATTTAGTTAATGTATAAATATCCCATTAAGATCTTGATGGTAATTTTTATACTATGGATCGAGTTTAAATCT

mRNA sequence

TTGACTTCAAATCTTCAATCAATCAATTCTTTCGAATCAATTTTCCCCAAATCCATGGAGGTCTCCGGCGCCGTATCCCGGCGGCTCCCCGGCCTCTTCCGCCGTGAGATTGCTCAGTCGTCTATGGAAGTCTCCGGCGCCGATGCTCGGCGGGCCCCCTGTAGCCTGTCCGTTTCCGGTGGCTCTGTTGGTATTGGAACTCGTGATCTTGGCCTCGTCGGTCGTCGGAATGATGATTCTTCTAGGTTTTGCGATGACGGTCATTTAAGGTATTATGTTGGACCGAGATGCCATGGGGGAAGATTGAAGAAGGAGAAGGAAGCGGTGAAGAAGAAATTGAAGTTGTTGAAAGGATTGTCCGGTGGTTTATCTGCGGCTTATGAATCGGGCTTCGTGTTTCGTTTTGATGATGGCTCGGTCGGTGAATTTCAAGGGGATCGCTTCTCTATCGAGGAAACAAGAGAGGCATTGGGAAGACAACTCCAACAGCTAAAAGCAGAGGAGAAAGAACAAAAAAGAAAGAGGAAGCTAGAGAAGGCCAAGCTGAAAGCAGCTCGAGCACAAACCATTCACGAGTCATCATCTTCATCCTCCGAATCCAGTGACAATGAAAGTAATGCAATCAACACAAGCTACCGAATAAGGAAGGCGCTTTCACAGCCATTCCCAGATCAATGGCAGACAAAAGCCATTCAAGGATCAGCACTGCCTCCTCCTTTGCAAACCCTGCAGCTTGGTTCAAACACCGAGAACACCGGTGTCGCCCGGCCGGCATCTGTAGGGAGGATTGAAGTGTGCATGGGGAACAAGTGCAAGAAGGCAGGGGCAGCAGCATTGATGGAGGAATTTGAGAGGGTGGTGGGTGCTCATGGAGTTGTTTGTGGGTGTAAATGCATGGGAAAATGCAGAGATGGACCAAATGTGAGAGTTTCTGGTTCTTTGGACAAGAAGCTCGCTCTGTAAATCCTCTGTGCATTGGAGTTGGAGTGGAGGATGTTGGTAGAATTGTAGCTGATTATCTAGGACAAGAAGGGCAGAAACAATCTAGGTTTGCACCTGCAATTTGATGCTTCATTCATTAGACGAGTTAACTTGTGCAGTTTGATTGAGTTTGTAAAAAGTTTAGGATAAGTTACAAATTTGATTGGAGAAAAGTTAGAATTAATTCTAACTATGAAAATCATATGAACTAAATTCTAACTTTCTCCAAACTATTAGGGATCAAATTTGTAATTTAACCAAAAGTTTTTCATCTTTCCTTTTGGTCACACTGAGTTTAAAAGGTTGGATTATACTGATTTGATAGTCGCAAGGAAATTTTTGGACCCAAACCAACTATTAGGATTGTAAATTTGTCGATTTCAATTGTAGGTAGGTGTTGAATCCAAAGTCAATTCAGGTTATTTCTTCATTTGTTCATTCTTGTTCAAAAAATAAAGAGGGTGTGAGTCTTTTGAATATTTATTTAGTTAATGTATAAATATCCCATTAAGATCTTGATGGTAATTTTTATACTATGGATCGAGTTTAAATCT

Coding sequence (CDS)

ATGGAGGTCTCCGGCGCCGTATCCCGGCGGCTCCCCGGCCTCTTCCGCCGTGAGATTGCTCAGTCGTCTATGGAAGTCTCCGGCGCCGATGCTCGGCGGGCCCCCTGTAGCCTGTCCGTTTCCGGTGGCTCTGTTGGTATTGGAACTCGTGATCTTGGCCTCGTCGGTCGTCGGAATGATGATTCTTCTAGGTTTTGCGATGACGGTCATTTAAGGTATTATGTTGGACCGAGATGCCATGGGGGAAGATTGAAGAAGGAGAAGGAAGCGGTGAAGAAGAAATTGAAGTTGTTGAAAGGATTGTCCGGTGGTTTATCTGCGGCTTATGAATCGGGCTTCGTGTTTCGTTTTGATGATGGCTCGGTCGGTGAATTTCAAGGGGATCGCTTCTCTATCGAGGAAACAAGAGAGGCATTGGGAAGACAACTCCAACAGCTAAAAGCAGAGGAGAAAGAACAAAAAAGAAAGAGGAAGCTAGAGAAGGCCAAGCTGAAAGCAGCTCGAGCACAAACCATTCACGAGTCATCATCTTCATCCTCCGAATCCAGTGACAATGAAAGTAATGCAATCAACACAAGCTACCGAATAAGGAAGGCGCTTTCACAGCCATTCCCAGATCAATGGCAGACAAAAGCCATTCAAGGATCAGCACTGCCTCCTCCTTTGCAAACCCTGCAGCTTGGTTCAAACACCGAGAACACCGGTGTCGCCCGGCCGGCATCTGTAGGGAGGATTGAAGTGTGCATGGGGAACAAGTGCAAGAAGGCAGGGGCAGCAGCATTGATGGAGGAATTTGAGAGGGTGGTGGGTGCTCATGGAGTTGTTTGTGGGTGTAAATGCATGGGAAAATGCAGAGATGGACCAAATGTGAGAGTTTCTGGTTCTTTGGACAAGAAGCTCGCTCTGTAA

Protein sequence

MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRNDDSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDGSVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSSESSDNESNAINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSLDKKLAL

Homology

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BLAST of Lcy10g010340 vs. ExPASy Swiss-Prot
Match: Q9C5W0 (Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.3e-32
Identity = 130/322 (40.37%), Postives = 168/322 (52.17%), Query Frame = 0


Query: 24  MEVSGADARRAPCSLSVSGGSV-GI--------GTRDLGLVGRRNDD---SSRFCDDGHL 83
           MEVSG   R+ PC   VS GSV G+         TR +G   RR      ++ F D GH+
Sbjct: 1   MEVSGVVLRQIPC---VSSGSVAGLRLVSEFSGNTRTVGFRTRRFRGIVCNNEFADKGHV 60

Query: 84  RYYVGP-RCHGGR-----LKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDGSVGEF 143
            YY+ P RC   +     ++KEK+A+KKK K+LK LS  L      GF    + G VGE 
Sbjct: 61  NYYIEPTRCGEEKEKVKVMEKEKKALKKKAKVLKSLSKNLDMFSSIGFGLDPEAGLVGEI 120

Query: 144 QGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHES-SSSSSESSD 203
           Q    +I E  E L +QL+QLKAEEK  K++RK EKAK KA +  T  +S SSSSSESSD
Sbjct: 121 QTK--TISEATEILVKQLEQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSD 180

Query: 204 NE---SNAINTSYRIRKALSQPFPDQWQTKAI----------------QGSALPPPLQTL 263
           ++      ++ S    KA  +P  +  Q +A                    AL   LQT 
Sbjct: 181 SDCDKGKVVDMSSLRNKA--KPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTS 240

Query: 264 QLGSNTENTG-----VARPASVG----RIEVCMGNKCKKAGAAALMEEFER-VVGAHGVV 298
            +  +  N G     V   + VG    R+EVCMG KCK++G A L++EF+R + G  G  
Sbjct: 241 TIFPSMANPGQTLKTVEAVSVVGLPLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSA 300

[Close]

BLAST of Lcy10g010340 vs. ExPASy TrEMBL
Match: A0A6J1GBG4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111452646 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 9.0e-125
Identity = 249/301 (82.72%), Postives = 258/301 (85.71%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGGSV   TRD GL  RRN 
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNAINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NTSYR RKA    LSQPF DQWQT AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 298
           AR +SVGR+EVCMGNKCKKAGAAALMEEFERV+GA  VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

[Close]

BLAST of Lcy10g010340 vs. ExPASy TrEMBL
Match: A0A6J1I582 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111470783 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 8.7e-120
Identity = 243/301 (80.73%), Postives = 252/301 (83.72%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAVSRRLPGL RREIAQSS+EVSG DARRAPCSLS+SGGSVG  TRD GL  RRN 
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSVEVSGHDARRAPCSLSISGGSVGTETRDFGLHVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGL G  SAA   GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLPGDSSAALAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR  T ++SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLPTAYDSSSSSS 180

Query: 181 ESSDNESNAINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    LSQ F DQWQ  AIQGSAL PPLQT QL SN  N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKASSPTLSQLFSDQWQANAIQGSALAPPLQTHQLNSNIGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 298
           AR ASVGR+EVCMGNKCKKAGAAALMEEF RV+GA  VVCGCKCMG+CRDGPNVRVSGSL
Sbjct: 241 ARSASVGRVEVCMGNKCKKAGAAALMEEFGRVMGADSVVCGCKCMGRCRDGPNVRVSGSL 300

[Close]

BLAST of Lcy10g010340 vs. ExPASy TrEMBL
Match: A0A6J1GV38 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111457454 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 1.2e-116
Identity = 232/297 (78.11%), Postives = 255/297 (85.86%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAV+RRL  LFR EIAQ S++V+ ADARRAPCSLSVSGGSV  GT  +GLVGRRN 
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQ-SVDVASADARRAPCSLSVSGGSVSTGTHVIGLVGRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRF D+GHLRYYVGP C G RLKKEKEAVKKKLKLLKGLSG  S  +ESG VFRFDD 
Sbjct: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDC 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHE-SSSSS 180
            +G+FQ DRFSIEETREAL +QLQQLK+EEK+QKR+RKLEKAKLKA R +TIHE SSSSS
Sbjct: 121 LIGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRVKTIHESSSSSS 180

Query: 181 SESSDNESNAINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARP 240
           SESSDNE    NTSY+IRKALSQPFPDQW+T  I+ S LPPPLQ+  L S+T+N GVAR 
Sbjct: 181 SESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARS 240

Query: 241 ASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 297
            SVGR+EVCMGNKCKKAGAAALMEEFERV+GA G VCGCKCMGKCRDGPNVRVSG+L
Sbjct: 241 VSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGAL 296

[Close]

BLAST of Lcy10g010340 vs. ExPASy TrEMBL
Match: A0A6J1IRZ4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111479426 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.2e-115
Identity = 231/300 (77.00%), Postives = 255/300 (85.00%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAV+RRL  LFR EI Q S++V+ ADARRAPCSLSVSGGSV  GTR +GLVGRRN 
Sbjct: 1   MEVSGAVTRRLSSLFRCEIVQ-SVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNV 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRF D+GHLRYYVGP C G RLKKEKEAVKKK KLLKGLSG  S  +ESG VFR DD 
Sbjct: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKFKLLKGLSGYSSTDHESGLVFRLDDC 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHE-SSSSS 180
            +G+ Q DRFSIEETREAL RQLQQLK+EEK+QKRKRKLEKAKLKA RA+TIH+ SSSSS
Sbjct: 121 LIGDSQADRFSIEETREALIRQLQQLKSEEKDQKRKRKLEKAKLKATRAKTIHKSSSSSS 180

Query: 181 SESSDNESNAINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARP 240
           SESSDNE   INT YRIRKALSQPFPDQW+T  I+ S LPPPLQ+  L S+T+N GVA+ 
Sbjct: 181 SESSDNEGYMINTRYRIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVAQS 240

Query: 241 ASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSLDKK 300
            SVGR+EVCMGNKCKKAGAAALMEEFERV+GA G VCGCKCMGKCRDGPNVRVSG+L ++
Sbjct: 241 VSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALARQ 299

[Close]

BLAST of Lcy10g010340 vs. ExPASy TrEMBL
Match: E5GC43 (Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 3.7e-110
Identity = 222/297 (74.75%), Postives = 244/297 (82.15%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEV GAVSR+LPGLFRR+ +Q S+EV  AD RR PCSLS+SG SVG  TR LGLV RR  
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQ-SVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRM 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRFCDDG+LRYYVGP C GG LKKEKEAVKKKLKLLKGLS   +  YES  +FRFD G
Sbjct: 61  DSSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLS---ATDYESSLLFRFDHG 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           S+ EFQ DRFSIE+TREAL RQLQQLK EEKEQKRK+KLEKAKLKAAR +TIH+SSSSSS
Sbjct: 121 SIDEFQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHDSSSSSS 180

Query: 181 ESSDNESNAINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARPA 240
           ESSDNE +    SY+++KALSQPFPDQ++T AI  S LP PLQT  L S TEN GV R  
Sbjct: 181 ESSDNEGHMNTRSYQLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRST 240

Query: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSLD 298
           SVGRIEVCMGNKCKKAGAAALMEEFERV+G    VCGCKCMGKCRDGPNVRV GS++
Sbjct: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSME 293

[Close]

BLAST of Lcy10g010340 vs. NCBI nr
Match: XP_022949236.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita moschata] >KAG7029039.1 Diacylglycerol O-acyltransferase 3, cytosolic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 456.4 bits (1173), Expect = 1.9e-124
Identity = 249/301 (82.72%), Postives = 258/301 (85.71%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGGSV   TRD GL  RRN 
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNAINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NTSYR RKA    LSQPF DQWQT AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 298
           AR +SVGR+EVCMGNKCKKAGAAALMEEFERV+GA  VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

[Close]

BLAST of Lcy10g010340 vs. NCBI nr
Match: XP_023539958.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-123
Identity = 248/301 (82.39%), Postives = 256/301 (85.05%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGGSV   TRD GL  RRN 
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNAINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    LSQPF DQWQ  AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKANSPSLSQPFSDQWQANAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 298
           AR ASVGR+EVCMGNKCKKAGAAALMEEFERV+GA  VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSASVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

[Close]

BLAST of Lcy10g010340 vs. NCBI nr
Match: KAG6597595.1 (Diacylglycerol O-acyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-123
Identity = 247/301 (82.06%), Postives = 257/301 (85.38%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGGSV   TRD GL  RRN 
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNAINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    +SQPF DQWQT AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKANSPSISQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 298
           AR +SVGR+EVCMGNKCKKAGAAALMEEFERV+GA  VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

[Close]

BLAST of Lcy10g010340 vs. NCBI nr
Match: XP_022972166.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita maxima])

HSP 1 Score: 439.9 bits (1130), Expect = 1.8e-119
Identity = 243/301 (80.73%), Postives = 252/301 (83.72%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAVSRRLPGL RREIAQSS+EVSG DARRAPCSLS+SGGSVG  TRD GL  RRN 
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSVEVSGHDARRAPCSLSISGGSVGTETRDFGLHVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGL G  SAA   GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLPGDSSAALAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR  T ++SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLPTAYDSSSSSS 180

Query: 181 ESSDNESNAINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    LSQ F DQWQ  AIQGSAL PPLQT QL SN  N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKASSPTLSQLFSDQWQANAIQGSALAPPLQTHQLNSNIGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 298
           AR ASVGR+EVCMGNKCKKAGAAALMEEF RV+GA  VVCGCKCMG+CRDGPNVRVSGSL
Sbjct: 241 ARSASVGRVEVCMGNKCKKAGAAALMEEFGRVMGADSVVCGCKCMGRCRDGPNVRVSGSL 300

[Close]

BLAST of Lcy10g010340 vs. NCBI nr
Match: KAG6582142.1 (Diacylglycerol O-acyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018541.1 Diacylglycerol O-acyltransferase 3, cytosolic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 433.0 bits (1112), Expect = 2.2e-117
Identity = 234/297 (78.79%), Postives = 257/297 (86.53%), Query Frame = 0


Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGSVGIGTRDLGLVGRRND 60
           MEVSGAV+RRL  LFR EIAQ S++V+ ADARRAPCSLSVSGGSV  GTR +GLVGRRN 
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQ-SVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRF D+GHLRYYVGP C G RLKKEKEAVKKKLKLLKGLSG  S  +ESG VFRFDD 
Sbjct: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDC 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHE-SSSSS 180
            +G+FQ DRFSIEETREAL +QLQQLK+EEK+QKR+RKLEKAKLKA RA+TIHE SSSSS
Sbjct: 121 LIGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSS 180

Query: 181 SESSDNESNAINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARP 240
           SESSDNE    NTSY+IRKALSQPFPDQW+T  I+ S LPPPLQ+  L S+T+N GVAR 
Sbjct: 181 SESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARS 240

Query: 241 ASVGRIEVCMGNKCKKAGAAALMEEFERVVGAHGVVCGCKCMGKCRDGPNVRVSGSL 297
            SVGR+EVCMGNKCKKAGAAALMEEFERV+GA G VCGCKCMGKCRDGPNVRVSG+L
Sbjct: 241 VSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGAL 296

[Close]

BLAST of Lcy10g010340 vs. TAIR 10
Match: AT1G48300.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 96 Blast hits to 87 proteins in 37 species: Archae - 0; Bacteria - 2; Metazoa - 12; Fungi - 2; Plants - 65; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 119.4 bits (298), Expect = 5.0e-27
Identity = 102/244 (41.80%), Postives = 132/244 (54.10%), Query Frame = 0


Query: 84  LKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDGSVGEFQGDRFSIEETREALGRQL 143
           ++KEK+A+KKK K+LK LS  L      GF    + G VGE Q    +I E  E L +QL
Sbjct: 1   MEKEKKALKKKAKVLKSLSKNLDMFSSIGFGLDPEAGLVGEIQTK--TISEATEILVKQL 60

Query: 144 QQLKAEEKEQKRKRKLEKAKLKAARAQTIHES-SSSSSESSDNE---SNAINTSYRIRKA 203
           +QLKAEEK  K++RK EKAK KA +  T  +S SSSSSESSD++      ++ S    KA
Sbjct: 61  EQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSDSDCDKGKVVDMSSLRNKA 120

Query: 204 LSQPFPDQWQTKAI----------------QGSALPPPLQTLQLGSNTENTG-----VAR 263
             +P  +  Q +A                    AL   LQT  +  +  N G     V  
Sbjct: 121 --KPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTSTIFPSMANPGQTLKTVEA 180

Query: 264 PASVG----RIEVCMGNKCKKAGAAALMEEFER-VVGAHGVVCGCKCMGKCRDGPNVRVS 298
            + VG    R+EVCMG KCK++G A L++EF+R + G  G    CKCMGKCRDGPNVRV 
Sbjct: 181 VSVVGLPLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSAVACKCMGKCRDGPNVRVV 240

The following BLAST results are available for this feature:

BLAST of Lcy10g010340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C5W0	2.3e-32	40.37	Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1...	[more]

BLAST of Lcy10g010340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBG4	9.0e-125	82.72	diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662...	[more]
A0A6J1I582	8.7e-120	80.73	diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1GV38	1.2e-116	78.11	diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662...	[more]
A0A6J1IRZ4	2.2e-115	77.00	diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 G...	[more]
E5GC43	3.7e-110	74.75	Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of Lcy10g010340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949236.1	1.9e-124	82.72	diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita moschata] >KAG7029...	[more]
XP_023539958.1	1.2e-123	82.39	diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita pepo subsp. pepo]	[more]
KAG6597595.1	1.2e-123	82.06	Diacylglycerol O-acyltransferase 3, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022972166.1	1.8e-119	80.73	diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita maxima]	[more]
KAG6582142.1	2.2e-117	78.79	Diacylglycerol O-acyltransferase 3, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Lcy10g010340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48300.1	5.0e-27	41.80	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 129..170
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 238..301 e-value: 1.5E-5 score: 27.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 170..188
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 148..188
None	No IPR available	CDD	cd02980	TRX_Fd_family	coord: 244..292 e-value: 1.45187E-9 score: 51.8592
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 243..295

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy10g010340.1	Lcy10g010340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019432	triglyceride biosynthetic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0004144	diacylglycerol O-acyltransferase activity