Lcy10g009840 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g009840
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptiongalactolipase DONGLE, chloroplastic-like
LocationChr10: 37235783 .. 37237487 (-)
RNA-Seq ExpressionLcy10g009840
SyntenyLcy10g009840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGCACAACATTAAAGCTCAATCCAATTCTCAATCATGTCACATTTTCTGAAACAACTCGCCCCATGTCTCATTCTTTCGGACAAGTCTCGCTCCCGAGAAAATCCGATAACGTTTCTTCTTCTTCTTCTTCGTCTTCGTGTTCTTCGTCGTCGTCTCGACGGTTGGTTTCGTCTTCTTCGGTTTTGGTACCGTTGATCGATGCGTTTTCTTCTTGTTCCACCGCGGCTGCCGTGTGCCACAAGGTGGCCGGTTTGGCGCGCTTGTGGCGGCAGATTCATGGCTGCAATGACTGGGAAGATCTCGTCGAGCCGACACTTCACCCGCTCTTGCGACGGGAGGTTATCCGCTACGGCGAGTTCGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCCAAACGGTATTTGACCTGCAAATTTGGGAAGAAGAGCTTGTTGAAGGAAGTGGGATTGGAGAGTTCGGGTTATGAAGTGACCAAATACATCTATGCTACCCCACCGGACATCAACATTCCTACCATCCAAAACTCGCCGCCGTCTTGTGGCCGGTGGATCGGTTACGTCGCTGTCTCCTCCGACGAGACCAGCAAGAGACTTGGGCGACGAGACATCGTCGTCACTTTCCGAGGTACTGTCACCAACTCCGAATGGATCGCCAATCTAATGAGCTCCCTCACGCCTGCCCGCCTCGACCCCCACAATCATCGGCCTGATGTTAAGGTTCGTTAATCTACCACTCAACTCAAAAGCTTAAATTTTGATTTATTTACCTTCTTTAACACAATTTAAAAATCAAACCCAACACCCAACAAGCAACTCTTCACTCTCAATTTCCTTGGAGATGAGTTACAATCAATAATCTTCACTCTCCTCCTATGAACTAGAAAAGAAAAGAAATGAGACCTCTTACTCTGATACCATGTTAAGAATCGAAAGTCTAAAACCTGATATCTGACAATAAATTTTTACTTTTTCCAGGTCGAATCAGGGTTCCTGACGCTCTACACGTGCGAGGAAACGAGCACGAAGTTCGGGCTCGAGAGCTGCCGGGAGCAGCTGCTCTCGGAAGTCTCGAGGCTCTTAAACAAGTACAAAAAGGAGGAGGTGAGCATAACCATGGCAGGGCACAGCATGGGCAGTGCACTAGCCCTTCTTCTAGCATATGACATAGCTGAGCTAGGGCTGAACAGAAGAACAAACAATGAAGTGGTTCCAGTTTCAGTGTTCTCCTTTGGAGGGCCAAGAGTTGGGAACTCAGGCTTCAAACAAAGATGTGAAGAATTGGGAGTCAAAGTTTTGAGAATTGTGAATGTGAATGATCCCATCACAAAGATGCCAGGTGTTTTGTTCAATGAAAATTTTAGGGTTTTTGGACTGCTCTTAAATGGAGGTGGAAACAATAGCTATGAACATGTTGGAGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATGCCCAAAAGAAGAAGCTCAAGATCAAGATGATGATGATGATGATGGGAGAAGAAAAATTATGATTAATGGTGGGGATTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCTCAGAGCTTGAACATGTTTCCATGGAGGAATCCAGTGAATAATTACTTGAGTCAATCACAAAATTAGGTCAAAAGAA

mRNA sequence

ATGGCGGGCACAACATTAAAGCTCAATCCAATTCTCAATCATGTCACATTTTCTGAAACAACTCGCCCCATGTCTCATTCTTTCGGACAAGTCTCGCTCCCGAGAAAATCCGATAACGTTTCTTCTTCTTCTTCTTCGTCTTCGTGTTCTTCGTCGTCGTCTCGACGGTTGGTTTCGTCTTCTTCGGTTTTGGTACCGTTGATCGATGCGTTTTCTTCTTGTTCCACCGCGGCTGCCGTGTGCCACAAGGTGGCCGGTTTGGCGCGCTTGTGGCGGCAGATTCATGGCTGCAATGACTGGGAAGATCTCGTCGAGCCGACACTTCACCCGCTCTTGCGACGGGAGGTTATCCGCTACGGCGAGTTCGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCCAAACGGTATTTGACCTGCAAATTTGGGAAGAAGAGCTTGTTGAAGGAAGTGGGATTGGAGAGTTCGGGTTATGAAGTGACCAAATACATCTATGCTACCCCACCGGACATCAACATTCCTACCATCCAAAACTCGCCGCCGTCTTGTGGCCGGTGGATCGGTTACGTCGCTGTCTCCTCCGACGAGACCAGCAAGAGACTTGGGCGACGAGACATCGTCGTCACTTTCCGAGGTACTGTCACCAACTCCGAATGGATCGCCAATCTAATGAGCTCCCTCACGCCTGCCCGCCTCGACCCCCACAATCATCGGCCTGATGTTAAGGTCGAATCAGGGTTCCTGACGCTCTACACGTGCGAGGAAACGAGCACGAAGTTCGGGCTCGAGAGCTGCCGGGAGCAGCTGCTCTCGGAAGTCTCGAGGCTCTTAAACAAGTACAAAAAGGAGGAGGTGAGCATAACCATGGCAGGGCACAGCATGGGCAGTGCACTAGCCCTTCTTCTAGCATATGACATAGCTGAGCTAGGGCTGAACAGAAGAACAAACAATGAAGTGGTTCCAGTTTCAGTGTTCTCCTTTGGAGGGCCAAGAGTTGGGAACTCAGGCTTCAAACAAAGATGTGAAGAATTGGGAGTCAAAGTTTTGAGAATTGTGAATGTGAATGATCCCATCACAAAGATGCCAGGTGTTTTGTTCAATGAAAATTTTAGGGTTTTTGGACTGCTCTTAAATGGAGGTGGAAACAATAGCTATGAACATGTTGGAGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATGCCCAAAAGAAGAAGCTCAAGATCAAGATGATGATGATGATGATGGGAGAAGAAAAATTATGATTAATGGTGGGGATTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCTCAGAGCTTGAACATGTTTCCATGGAGGAATCCAGTGAATAATTACTTGAGTCAATCACAAAATTAGGTCAAAAGAA

Coding sequence (CDS)

ATGGCGGGCACAACATTAAAGCTCAATCCAATTCTCAATCATGTCACATTTTCTGAAACAACTCGCCCCATGTCTCATTCTTTCGGACAAGTCTCGCTCCCGAGAAAATCCGATAACGTTTCTTCTTCTTCTTCTTCGTCTTCGTGTTCTTCGTCGTCGTCTCGACGGTTGGTTTCGTCTTCTTCGGTTTTGGTACCGTTGATCGATGCGTTTTCTTCTTGTTCCACCGCGGCTGCCGTGTGCCACAAGGTGGCCGGTTTGGCGCGCTTGTGGCGGCAGATTCATGGCTGCAATGACTGGGAAGATCTCGTCGAGCCGACACTTCACCCGCTCTTGCGACGGGAGGTTATCCGCTACGGCGAGTTCGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCCAAACGGTATTTGACCTGCAAATTTGGGAAGAAGAGCTTGTTGAAGGAAGTGGGATTGGAGAGTTCGGGTTATGAAGTGACCAAATACATCTATGCTACCCCACCGGACATCAACATTCCTACCATCCAAAACTCGCCGCCGTCTTGTGGCCGGTGGATCGGTTACGTCGCTGTCTCCTCCGACGAGACCAGCAAGAGACTTGGGCGACGAGACATCGTCGTCACTTTCCGAGGTACTGTCACCAACTCCGAATGGATCGCCAATCTAATGAGCTCCCTCACGCCTGCCCGCCTCGACCCCCACAATCATCGGCCTGATGTTAAGGTCGAATCAGGGTTCCTGACGCTCTACACGTGCGAGGAAACGAGCACGAAGTTCGGGCTCGAGAGCTGCCGGGAGCAGCTGCTCTCGGAAGTCTCGAGGCTCTTAAACAAGTACAAAAAGGAGGAGGTGAGCATAACCATGGCAGGGCACAGCATGGGCAGTGCACTAGCCCTTCTTCTAGCATATGACATAGCTGAGCTAGGGCTGAACAGAAGAACAAACAATGAAGTGGTTCCAGTTTCAGTGTTCTCCTTTGGAGGGCCAAGAGTTGGGAACTCAGGCTTCAAACAAAGATGTGAAGAATTGGGAGTCAAAGTTTTGAGAATTGTGAATGTGAATGATCCCATCACAAAGATGCCAGGTGTTTTGTTCAATGAAAATTTTAGGGTTTTTGGACTGCTCTTAAATGGAGGTGGAAACAATAGCTATGAACATGTTGGAGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATGCCCAAAAGAAGAAGCTCAAGATCAAGATGATGATGATGATGATGGGAGAAGAAAAATTATGATTAATGGTGGGGATTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCTCAGAGCTTGAACATGTTTCCATGGAGGAATCCAGTGAATAATTACTTGAGTCAATCACAAAATTAG

Protein sequence

MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDNVSSSSSSSSCSSSSSRRLVSSSSVLVPLIDAFSSCSTAAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRCPKEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPVNNYLSQSQN
Homology
BLAST of Lcy10g009840 vs. ExPASy Swiss-Prot
Match: Q9SIN9 (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5 PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 5.3e-137
Identity = 273/487 (56.06%), Postives = 333/487 (68.38%), Query Frame = 0

Query: 9   NPILNHVTFSETTRPMSHSFGQVSL--------PRKSDNVSSSSSSSSCSSSSSRRLVSS 68
           NP + H  F     P    F   +L        P++    +SSSSSS  +       V+S
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSSSSSSLLAPVILNSPVAS 65

Query: 69  SSVLVPLIDAFSSCSTAAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREVIRYG 128
           SS           CS+ AA       L+R+WR+I GCN+W+DL+EP L+PLL++E+ RYG
Sbjct: 66  SSPPPIYCAPKFPCSSGAA----TVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYG 125

Query: 129 EFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEVTKYIYATPP-DINIPTI 188
             V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI  I
Sbjct: 126 NLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPI 185

Query: 189 QNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDPHN 248
           QN      RW+GYVA SSD++ KRLGRRDIVVTFRGTVTN EW+AN MSSLTPAR  PHN
Sbjct: 186 QNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHN 245

Query: 249 HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSMGS 308
            R DVKVESGFL+LYT +E+ +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSMGS
Sbjct: 246 PRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGS 305

Query: 309 ALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVND 368
           +LA LLAYDIAELGLNRR     +PV+VFSF GPRVGN  FK+RCEELGVKVLRI NVND
Sbjct: 306 SLAQLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVND 365

Query: 369 PITKMPGVLFNENFRVFGLLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYISLL 428
           P+TK+PGVLFNENFRV G       + S Y HVGVEL LDFF++QN SCVHDL+TYI LL
Sbjct: 366 PVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYIDLL 425

Query: 429 RCPKEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQSL------NMFPWRNPVNN 479
              +  ++  D D+D+          D  N A+EFL +N + +       M  W N V+ 
Sbjct: 426 NQRRTNSRSVDSDEDE----------DSDNVALEFLKTNGEKMMFLKRQRMMYWSNAVDL 477

BLAST of Lcy10g009840 vs. ExPASy Swiss-Prot
Match: Q9MA46 (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.6e-136
Identity = 270/463 (58.32%), Postives = 333/463 (71.92%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDN---VSSSSSSSSCSSSSSRRL 60
           MA      NPI +     + T    H+    S+ + +     VSSS+ S   SSS     
Sbjct: 1   MAAKVFTQNPIYSQSLVRDKTPQQKHNLDHFSISQHTSKRLVVSSSTMSPPISSSPLSLP 60

Query: 61  VSSSSVLVPLIDAFSSCSTAAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREVI 120
            SSSS  +P        S A AV      L+R+WR+I G N+WE+L+EP L P+L++E+ 
Sbjct: 61  SSSSSQAIP-------PSRAPAV---TLPLSRVWREIQGSNNWENLIEP-LSPILQQEIT 120

Query: 121 RYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGL-ESSGYEVTKYIYATPPDINIP 180
           RYG  ++A YK FDL+PNSKRYL+CK+GKK+LLKE G+ +  GY+VTKYIYAT PDIN+ 
Sbjct: 121 RYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYAT-PDINLN 180

Query: 181 TIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDP 240
            I+N  P+  RWIGYVAVSSDE+ KRLGRRDI+VTFRGTVTN EW+ANL SSLTPARLDP
Sbjct: 181 PIKNE-PNRARWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPARLDP 240

Query: 241 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSM 300
           HN RPDVKVESGFL LYT  E+ +KFGLESCREQLLSE+SRL+NK+K EE+SIT+AGHSM
Sbjct: 241 HNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEEISITLAGHSM 300

Query: 301 GSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 360
           GS+LA LLAYDIAELG+N+R + + VPV+VFSF GPRVGN GFK+RCEELGVKVLRI NV
Sbjct: 301 GSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAGPRVGNLGFKKRCEELGVKVLRITNV 360

Query: 361 NDPITKMPGVLFNENFRVFGLLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYIS 420
           NDPITK+PG LFNENFR  G +     + S Y HVGVEL LDFF++QN SCVHDLETYI+
Sbjct: 361 NDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHVGVELTLDFFDVQNISCVHDLETYIT 420

Query: 421 LLRCPKEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQ 459
           L+  P+      ++D+          GG+F+N+  E + S  +
Sbjct: 421 LVNRPRCSKLAVNEDN---------FGGEFLNRTSELMFSKGR 441

BLAST of Lcy10g009840 vs. ExPASy Swiss-Prot
Match: A2WY00 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=EG1 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.2e-115
Identity = 216/367 (58.86%), Postives = 271/367 (73.84%), Query Frame = 0

Query: 56  RLVSSSSVLVPLIDAFSSCSTAAAVCHKVA-GLARLWRQIHGCNDWEDLVEPTLHPLLRR 115
           R V+++   V + DA  +         K A  +A +WR++ GC DWE ++EP  HP+LR 
Sbjct: 24  RAVAAAGGAVAVRDAVVAPVARRGAARKTAETVAGMWREVQGCGDWEGMLEPAPHPVLRG 83

Query: 116 EVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDIN 175
           EV RYGE V ACYKAFDLDP S+RYL CK+G++ +L+EVG+  +GYEVT+YIYA   D++
Sbjct: 84  EVARYGELVGACYKAFDLDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-ADVS 143

Query: 176 IPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARL 235
           +PT++ S    GRWIGYVAVS+DE S+RLGRRD++V+FRGTVT +EW+ANLMSSL  ARL
Sbjct: 144 VPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARL 203

Query: 236 DPHNHRPDVKVESGFLTLYTCEETSTKF-GLESCREQLLSEVSRLLNKYK--KEEVSITM 295
           DP + RPDVKVESGFL+LYT  + + +F G  SCREQLL EVSRL+  Y    E+VS+T+
Sbjct: 204 DPCDPRPDVKVESGFLSLYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSVTL 263

Query: 296 AGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVL 355
           AGHSMGSALALL AYD+AELGLNR       PV+VFSFGGPRVGN+ FK RC+ELGVK L
Sbjct: 264 AGHSMGSALALLSAYDLAELGLNR-----AAPVTVFSFGGPRVGNAAFKARCDELGVKAL 323

Query: 356 RIVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLE 415
           R+ NV+DPITK+PGV  NE       +L    ++ Y HVGVEL LDFF + + + VHDL 
Sbjct: 324 RVTNVHDPITKLPGVFLNE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHDLA 381

Query: 416 TYISLLR 419
           TYISLLR
Sbjct: 384 TYISLLR 381

BLAST of Lcy10g009840 vs. ExPASy Swiss-Prot
Match: Q8S1D9 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=EG1 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.2e-115
Identity = 216/367 (58.86%), Postives = 271/367 (73.84%), Query Frame = 0

Query: 56  RLVSSSSVLVPLIDAFSSCSTAAAVCHKVA-GLARLWRQIHGCNDWEDLVEPTLHPLLRR 115
           R V+++   V + DA  +         K A  +A +WR++ GC DWE ++EP  HP+LR 
Sbjct: 24  RAVAAAGGAVAVRDAVVAPVARRGAARKTAETVAGMWREVQGCGDWEGMLEPAPHPVLRG 83

Query: 116 EVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDIN 175
           EV RYGE V ACYKAFDLDP S+RYL CK+G++ +L+EVG+  +GYEVT+YIYA   D++
Sbjct: 84  EVARYGELVGACYKAFDLDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-ADVS 143

Query: 176 IPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARL 235
           +PT++ S    GRWIGYVAVS+DE S+RLGRRD++V+FRGTVT +EW+ANLMSSL  ARL
Sbjct: 144 VPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARL 203

Query: 236 DPHNHRPDVKVESGFLTLYTCEETSTKF-GLESCREQLLSEVSRLLNKYK--KEEVSITM 295
           DP + RPDVKVESGFL+LYT  + + +F G  SCREQLL EVSRL+  Y    E+VS+T+
Sbjct: 204 DPCDPRPDVKVESGFLSLYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSVTL 263

Query: 296 AGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVL 355
           AGHSMGSALALL AYD+AELGLNR       PV+VFSFGGPRVGN+ FK RC+ELGVK L
Sbjct: 264 AGHSMGSALALLSAYDLAELGLNR-----AAPVTVFSFGGPRVGNAAFKARCDELGVKAL 323

Query: 356 RIVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLE 415
           R+ NV+DPITK+PGV  NE       +L    ++ Y HVGVEL LDFF + + + VHDL 
Sbjct: 324 RVTNVHDPITKLPGVFLNE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHDLA 381

Query: 416 TYISLLR 419
           TYISLLR
Sbjct: 384 TYISLLR 381

BLAST of Lcy10g009840 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 2.3e-79
Identity = 177/399 (44.36%), Postives = 239/399 (59.90%), Query Frame = 0

Query: 91  WRQIHGCNDWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 150
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 151 KEVGLESSGYEVTKYIYATPPDINIPTIQNSP------PSCGRWIGYVAVSSDETSK-RL 210
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS DETS+ RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 211 GRRDIVVTFRGTVTNSEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 270
           GRRDI + +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 271 GLESCREQLLSEVSRLLNKYKKE---EVSITMAGHSMGSALALLLAYDIAELGLNRRTNN 330
              S REQ+L+EV RL+ ++  +   ++SIT+ GHS+G ALA+L AYDIAE+ LNR    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 331 EVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGLLL 390
           +V+PV+V ++GGPRVGN  F++R EELGVKV+R+VNV+D + K PG+  NE+     + +
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 391 NGGGNNSYEHVGVELVLDFFNMQ------NPSCVHDLETYISLLRCPKEEAQDQDDDDDD 450
             G    Y HVG EL LD  N        + S  H+LE  + LL          D     
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL----------DGYHGK 464

Query: 451 GRRKIMINGGD--FINKAMEFLCSNAQSLNMFPWRNPVN 471
           G R ++ +G D   +NKA +FL  + Q +  F WR   N
Sbjct: 465 GERFVLSSGRDHALVNKASDFLKEHLQ-IPPF-WRQDAN 489

BLAST of Lcy10g009840 vs. ExPASy TrEMBL
Match: A0A0A0L8A7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE=4 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.4e-244
Identity = 437/487 (89.73%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSD-NVSSSSSSSSCSSSSSRRLVS 60
           MA TTLKLN I NHVTF ETTRPMSHSFGQVS+PRKSD NVSSSSSSSS SS+S R  VS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLID-----AFSSCST--AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSSVLVPL+D     +FSSCST  AAA+  KVA LARLWRQIHGCNDWEDLVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPA 240
           INIP IQNSPPSCGRWIGYVAVSSDETSKRLGRRDI++TFRGTVTN EWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSEVSRLLNKYK+EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           IVNVNDPITKMPGVLFNENFRVFG LLNGG NNSYEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCP-KEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPVNN 479
           YISLLRCP K+E +D+D+D+D GR KI+IN G+FINKAMEFLCSNAQSLNMFPWRNP+ N
Sbjct: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPM-N 480

BLAST of Lcy10g009840 vs. ExPASy TrEMBL
Match: A0A5A7VKE2 (Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00300 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 435/493 (88.24%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSD-NVSSSSSSSSCSSSSSRRL-V 60
           MA TTLKLN I NHVTF ETTRPMSHSFGQVS+PRKSD NVSSSSSSSS SSS+SRRL V
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLID-------AFSSCST--AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLH 120
           SSSSVLVPL+D       +FSSCST  AAA+  KVA LARLWRQIHGCNDWEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSL 240
           PPDINIP IQNSPPSCGRWIGYVAVSSDETSKRLGRRDI++TFRGTVT+ EWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSEVSR+LNKYK+EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR FG LLNGG NNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRCPK-EEAQDQDDD---DDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPW 479
           LETYISLLRCPK +E + QD+D   D +GRRKI IN G+FINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

BLAST of Lcy10g009840 vs. ExPASy TrEMBL
Match: A0A1S3AXV1 (galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 435/493 (88.24%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSD-NVSSSSSSSSCSSSSSRRL-V 60
           MA TTLKLN I NHVTF ETTRPMSHSFGQVS+PRKSD NVSSSSSSSS SSS+SRRL V
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLID-------AFSSCST--AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLH 120
           SSSSVLVPL+D       +FSSCST  AAA+  KVA LARLWRQIHGCNDWEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSL 240
           PPDINIP IQNSPPSCGRWIGYVAVSSDETSKRLGRRDI++TFRGTVT+ EWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSEVSR+LNKYK+EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR FG LLNGG NNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRCPK-EEAQDQDDD---DDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPW 479
           LETYISLLRCPK +E + QD+D   D +GRRKI IN G+FINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

BLAST of Lcy10g009840 vs. ExPASy TrEMBL
Match: A0A6J1GW19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458003 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.1e-220
Identity = 409/489 (83.64%), Postives = 424/489 (86.71%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDNVSSSSSSSSCSSSSSRRLVSS 60
           MA TTLKLN  +NHVTFSETTRP+SHSFGQVSLPRKSD+VSS+S  S  SS       SS
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSS-------SS 60

Query: 61  SSVLVPLIDAFSSCST--------AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSVLVPLID FSS S+        A A+ HKVAGLARLWRQIHGCNDWE LVEPTLHPLL
Sbjct: 61  SSVLVPLIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPA 240
           INIP IQNSPPSCGRWIGYVAVSSDETS+RLGRRD+V+TFRGTVTNSEWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           +VNVNDPITKMPG        VFG          YEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 VVNVNDPITKMPG--------VFG---------GYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCPKEEAQDQDDDD---DDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPV 479
           YISLLR PKEE Q+ DDDD    +GRRK  INGGDFINKAMEFL     S NMFPWRNP+
Sbjct: 421 YISLLRYPKEEHQEHDDDDHHHHNGRRK-AINGGDFINKAMEFL-----SFNMFPWRNPM 458

BLAST of Lcy10g009840 vs. ExPASy TrEMBL
Match: A0A6J1IP19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479244 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.2e-220
Identity = 404/482 (83.82%), Postives = 420/482 (87.14%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDNVSSSSSSSSCSSSSSRRLVSS 60
           MA TTLKLN  +NHVTFSETTRPMSHSFGQVSLPRKSD+VSS+S  S  SS       SS
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSDHVSSTSRRSVSSS-------SS 60

Query: 61  SSVLVPLIDAFSSCSTAA----AVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREV 120
           SSVLVPLID FSS S++A    A+ HK+AGLARLWRQIHGCNDWE LVEPTLHPLLRREV
Sbjct: 61  SSVLVPLIDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREV 120

Query: 121 IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIP 180
           IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIP
Sbjct: 121 IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIP 180

Query: 181 TIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDP 240
            IQNSPPSCGRWIGYVAVSSDETSKRLGRRD+V+TFRGTVTNSEWIANLMSSLTPARLDP
Sbjct: 181 PIQNSPPSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDP 240

Query: 241 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSM 300
           HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK EEVSITMAGHSM
Sbjct: 241 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSM 300

Query: 301 GSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 360
           GSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNV
Sbjct: 301 GSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNV 360

Query: 361 NDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISL 420
           NDPITK+PG        VFG          YEHVGVELVLDFFNMQNPSCVHDLETYISL
Sbjct: 361 NDPITKIPG--------VFG---------GYEHVGVELVLDFFNMQNPSCVHDLETYISL 420

Query: 421 LRCPKEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPVNNYLSQS 479
           LR PKEE Q  DDD     R+ +INGGDFINKAMEFL     S NM PWRNP+ NYLSQS
Sbjct: 421 LRYPKEEHQKHDDDHHHNGRRKVINGGDFINKAMEFL-----SFNMLPWRNPM-NYLSQS 452

BLAST of Lcy10g009840 vs. NCBI nr
Match: XP_004148168.2 (galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical protein Csa_009777 [Cucumis sativus])

HSP 1 Score: 855.1 bits (2208), Expect = 2.8e-244
Identity = 437/487 (89.73%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSD-NVSSSSSSSSCSSSSSRRLVS 60
           MA TTLKLN I NHVTF ETTRPMSHSFGQVS+PRKSD NVSSSSSSSS SS+S R  VS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLID-----AFSSCST--AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSSVLVPL+D     +FSSCST  AAA+  KVA LARLWRQIHGCNDWEDLVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPA 240
           INIP IQNSPPSCGRWIGYVAVSSDETSKRLGRRDI++TFRGTVTN EWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSEVSRLLNKYK+EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           IVNVNDPITKMPGVLFNENFRVFG LLNGG NNSYEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCP-KEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPVNN 479
           YISLLRCP K+E +D+D+D+D GR KI+IN G+FINKAMEFLCSNAQSLNMFPWRNP+ N
Sbjct: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPM-N 480

BLAST of Lcy10g009840 vs. NCBI nr
Match: XP_008439078.1 (PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo] >KAA0067660.1 galactolipase DONGLE [Cucumis melo var. makuwa] >TYK23664.1 galactolipase DONGLE [Cucumis melo var. makuwa])

HSP 1 Score: 841.3 bits (2172), Expect = 4.2e-240
Identity = 435/493 (88.24%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSD-NVSSSSSSSSCSSSSSRRL-V 60
           MA TTLKLN I NHVTF ETTRPMSHSFGQVS+PRKSD NVSSSSSSSS SSS+SRRL V
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLID-------AFSSCST--AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLH 120
           SSSSVLVPL+D       +FSSCST  AAA+  KVA LARLWRQIHGCNDWEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSL 240
           PPDINIP IQNSPPSCGRWIGYVAVSSDETSKRLGRRDI++TFRGTVT+ EWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSEVSR+LNKYK+EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR FG LLNGG NNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRCPK-EEAQDQDDD---DDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPW 479
           LETYISLLRCPK +E + QD+D   D +GRRKI IN G+FINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

BLAST of Lcy10g009840 vs. NCBI nr
Match: XP_038886126.1 (galactolipase DONGLE, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 825.5 bits (2131), Expect = 2.4e-235
Identity = 426/485 (87.84%), Postives = 441/485 (90.93%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDNVSSSSSSSSCSSSSSRRLVSS 60
           MA TTLKLN I NHVTF ETTRPMSHSFGQV +P K +NV       S SSSS R  VSS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVLIPIKPNNV------LSLSSSSRRLSVSS 60

Query: 61  SSVLVPLIDAF-SSCST--AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREVI 120
           S VLVPL+D F SSCST  AAA+ HKVA LA LWRQIHGCNDWEDL+EPTLHPLLRRE+I
Sbjct: 61  SLVLVPLMDVFSSSCSTAAAAAMVHKVASLAHLWRQIHGCNDWEDLMEPTLHPLLRREII 120

Query: 121 RYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPT 180
           RYGEFVTACYKAFDLDPNSKRYLTCKFGKK+LL+EVGLESSGYEVTKYIYATPPDINI  
Sbjct: 121 RYGEFVTACYKAFDLDPNSKRYLTCKFGKKNLLREVGLESSGYEVTKYIYATPPDINISP 180

Query: 181 IQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDPH 240
           IQNSPPSCGRWIGYVAVSSDETSKRLGRRDIV+TFRGTVTN EWIANLMSSLT ARLDPH
Sbjct: 181 IQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVITFRGTVTNPEWIANLMSSLTLARLDPH 240

Query: 241 NHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSMG 300
           NHRPDVKVESGFLTLYT EETS KFGLESCREQLLSEVSRLLNKYK+EEVSITMAGHSMG
Sbjct: 241 NHRPDVKVESGFLTLYTSEETSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMAGHSMG 300

Query: 301 SALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVN 360
           SALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RCEELGVKVLRIVNVN
Sbjct: 301 SALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVN 360

Query: 361 DPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLL 420
           DPITKMPGVLFNENFRVFG LLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLL
Sbjct: 361 DPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLL 420

Query: 421 RC-PKEEAQDQDDDDDD---GRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPVNNYL 479
           RC PK+E   QD+DDDD   GRRKI+IN G+FINKAMEFLCSNAQ LNMFPWR PV NYL
Sbjct: 421 RCDPKQEEDHQDNDDDDHNNGRRKIIINSGEFINKAMEFLCSNAQILNMFPWRIPV-NYL 478

BLAST of Lcy10g009840 vs. NCBI nr
Match: XP_022956251.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 774.2 bits (1998), Expect = 6.3e-220
Identity = 409/489 (83.64%), Postives = 424/489 (86.71%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDNVSSSSSSSSCSSSSSRRLVSS 60
           MA TTLKLN  +NHVTFSETTRP+SHSFGQVSLPRKSD+VSS+S  S  SS       SS
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSS-------SS 60

Query: 61  SSVLVPLIDAFSSCST--------AAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSVLVPLID FSS S+        A A+ HKVAGLARLWRQIHGCNDWE LVEPTLHPLL
Sbjct: 61  SSVLVPLIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPTIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPA 240
           INIP IQNSPPSCGRWIGYVAVSSDETS+RLGRRD+V+TFRGTVTNSEWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           +VNVNDPITKMPG        VFG          YEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 VVNVNDPITKMPG--------VFG---------GYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCPKEEAQDQDDDD---DDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPV 479
           YISLLR PKEE Q+ DDDD    +GRRK  INGGDFINKAMEFL     S NMFPWRNP+
Sbjct: 421 YISLLRYPKEEHQEHDDDDHHHHNGRRK-AINGGDFINKAMEFL-----SFNMFPWRNPM 458

BLAST of Lcy10g009840 vs. NCBI nr
Match: XP_022979567.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 404/482 (83.82%), Postives = 420/482 (87.14%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDNVSSSSSSSSCSSSSSRRLVSS 60
           MA TTLKLN  +NHVTFSETTRPMSHSFGQVSLPRKSD+VSS+S  S  SS       SS
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSDHVSSTSRRSVSSS-------SS 60

Query: 61  SSVLVPLIDAFSSCSTAA----AVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREV 120
           SSVLVPLID FSS S++A    A+ HK+AGLARLWRQIHGCNDWE LVEPTLHPLLRREV
Sbjct: 61  SSVLVPLIDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREV 120

Query: 121 IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIP 180
           IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIP
Sbjct: 121 IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIP 180

Query: 181 TIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDP 240
            IQNSPPSCGRWIGYVAVSSDETSKRLGRRD+V+TFRGTVTNSEWIANLMSSLTPARLDP
Sbjct: 181 PIQNSPPSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDP 240

Query: 241 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSM 300
           HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK EEVSITMAGHSM
Sbjct: 241 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSM 300

Query: 301 GSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 360
           GSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNV
Sbjct: 301 GSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNV 360

Query: 361 NDPITKMPGVLFNENFRVFGLLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISL 420
           NDPITK+PG        VFG          YEHVGVELVLDFFNMQNPSCVHDLETYISL
Sbjct: 361 NDPITKIPG--------VFG---------GYEHVGVELVLDFFNMQNPSCVHDLETYISL 420

Query: 421 LRCPKEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQSLNMFPWRNPVNNYLSQS 479
           LR PKEE Q  DDD     R+ +INGGDFINKAMEFL     S NM PWRNP+ NYLSQS
Sbjct: 421 LRYPKEEHQKHDDDHHHNGRRKVINGGDFINKAMEFL-----SFNMLPWRNPM-NYLSQS 452

BLAST of Lcy10g009840 vs. TAIR 10
Match: AT2G31690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 489.2 bits (1258), Expect = 3.8e-138
Identity = 273/487 (56.06%), Postives = 333/487 (68.38%), Query Frame = 0

Query: 9   NPILNHVTFSETTRPMSHSFGQVSL--------PRKSDNVSSSSSSSSCSSSSSRRLVSS 68
           NP + H  F     P    F   +L        P++    +SSSSSS  +       V+S
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSSSSSSLLAPVILNSPVAS 65

Query: 69  SSVLVPLIDAFSSCSTAAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREVIRYG 128
           SS           CS+ AA       L+R+WR+I GCN+W+DL+EP L+PLL++E+ RYG
Sbjct: 66  SSPPPIYCAPKFPCSSGAA----TVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYG 125

Query: 129 EFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEVTKYIYATPP-DINIPTI 188
             V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI  I
Sbjct: 126 NLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPI 185

Query: 189 QNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDPHN 248
           QN      RW+GYVA SSD++ KRLGRRDIVVTFRGTVTN EW+AN MSSLTPAR  PHN
Sbjct: 186 QNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHN 245

Query: 249 HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSMGS 308
            R DVKVESGFL+LYT +E+ +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSMGS
Sbjct: 246 PRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGS 305

Query: 309 ALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVND 368
           +LA LLAYDIAELGLNRR     +PV+VFSF GPRVGN  FK+RCEELGVKVLRI NVND
Sbjct: 306 SLAQLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVND 365

Query: 369 PITKMPGVLFNENFRVFGLLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYISLL 428
           P+TK+PGVLFNENFRV G       + S Y HVGVEL LDFF++QN SCVHDL+TYI LL
Sbjct: 366 PVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYIDLL 425

Query: 429 RCPKEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQSL------NMFPWRNPVNN 479
              +  ++  D D+D+          D  N A+EFL +N + +       M  W N V+ 
Sbjct: 426 NQRRTNSRSVDSDEDE----------DSDNVALEFLKTNGEKMMFLKRQRMMYWSNAVDL 477

BLAST of Lcy10g009840 vs. TAIR 10
Match: AT1G05800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 487.6 bits (1254), Expect = 1.1e-137
Identity = 270/463 (58.32%), Postives = 333/463 (71.92%), Query Frame = 0

Query: 1   MAGTTLKLNPILNHVTFSETTRPMSHSFGQVSLPRKSDN---VSSSSSSSSCSSSSSRRL 60
           MA      NPI +     + T    H+    S+ + +     VSSS+ S   SSS     
Sbjct: 1   MAAKVFTQNPIYSQSLVRDKTPQQKHNLDHFSISQHTSKRLVVSSSTMSPPISSSPLSLP 60

Query: 61  VSSSSVLVPLIDAFSSCSTAAAVCHKVAGLARLWRQIHGCNDWEDLVEPTLHPLLRREVI 120
            SSSS  +P        S A AV      L+R+WR+I G N+WE+L+EP L P+L++E+ 
Sbjct: 61  SSSSSQAIP-------PSRAPAV---TLPLSRVWREIQGSNNWENLIEP-LSPILQQEIT 120

Query: 121 RYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGL-ESSGYEVTKYIYATPPDINIP 180
           RYG  ++A YK FDL+PNSKRYL+CK+GKK+LLKE G+ +  GY+VTKYIYAT PDIN+ 
Sbjct: 121 RYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYAT-PDINLN 180

Query: 181 TIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDP 240
            I+N  P+  RWIGYVAVSSDE+ KRLGRRDI+VTFRGTVTN EW+ANL SSLTPARLDP
Sbjct: 181 PIKNE-PNRARWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPARLDP 240

Query: 241 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKKEEVSITMAGHSM 300
           HN RPDVKVESGFL LYT  E+ +KFGLESCREQLLSE+SRL+NK+K EE+SIT+AGHSM
Sbjct: 241 HNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEEISITLAGHSM 300

Query: 301 GSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 360
           GS+LA LLAYDIAELG+N+R + + VPV+VFSF GPRVGN GFK+RCEELGVKVLRI NV
Sbjct: 301 GSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAGPRVGNLGFKKRCEELGVKVLRITNV 360

Query: 361 NDPITKMPGVLFNENFRVFGLLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYIS 420
           NDPITK+PG LFNENFR  G +     + S Y HVGVEL LDFF++QN SCVHDLETYI+
Sbjct: 361 NDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHVGVELTLDFFDVQNISCVHDLETYIT 420

Query: 421 LLRCPKEEAQDQDDDDDDGRRKIMINGGDFINKAMEFLCSNAQ 459
           L+  P+      ++D+          GG+F+N+  E + S  +
Sbjct: 421 LVNRPRCSKLAVNEDN---------FGGEFLNRTSELMFSKGR 441

BLAST of Lcy10g009840 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-80
Identity = 177/399 (44.36%), Postives = 239/399 (59.90%), Query Frame = 0

Query: 91  WRQIHGCNDWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 150
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 151 KEVGLESSGYEVTKYIYATPPDINIPTIQNSP------PSCGRWIGYVAVSSDETSK-RL 210
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS DETS+ RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 211 GRRDIVVTFRGTVTNSEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 270
           GRRDI + +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 271 GLESCREQLLSEVSRLLNKYKKE---EVSITMAGHSMGSALALLLAYDIAELGLNRRTNN 330
              S REQ+L+EV RL+ ++  +   ++SIT+ GHS+G ALA+L AYDIAE+ LNR    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 331 EVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGLLL 390
           +V+PV+V ++GGPRVGN  F++R EELGVKV+R+VNV+D + K PG+  NE+     + +
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 391 NGGGNNSYEHVGVELVLDFFNMQ------NPSCVHDLETYISLLRCPKEEAQDQDDDDDD 450
             G    Y HVG EL LD  N        + S  H+LE  + LL          D     
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL----------DGYHGK 464

Query: 451 GRRKIMINGGD--FINKAMEFLCSNAQSLNMFPWRNPVN 471
           G R ++ +G D   +NKA +FL  + Q +  F WR   N
Sbjct: 465 GERFVLSSGRDHALVNKASDFLKEHLQ-IPPF-WRQDAN 489

BLAST of Lcy10g009840 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 3.6e-80
Identity = 172/385 (44.68%), Postives = 233/385 (60.52%), Query Frame = 0

Query: 91  WRQIHGCNDWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 150
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP S+   +C+F ++ L 
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 151 KEVGLESSGYEVTKYIYATPPDINIPTIQNSP------PSCGRWIGYVAVSSDE--TSKR 210
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D   T  R
Sbjct: 149 DSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 211 LGRRDIVVTFRGTVTNSEWIANLMSSLTPARLDPHN-HRPDVKVESGFLTLYTCEETSTK 270
           LGRRDI + +RGTVT  EWIA+L   L P   +      P VK ESGFL LYT ++TS  
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 271 FGLESCREQLLSEVSRLLNKY---KKEEVSITMAGHSMGSALALLLAYDIAELGLNRRTN 330
           F   S REQ+L+EV RL+ +Y   + EE+SIT+ GHS+G ALA+L AYD+AE+G+NR   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 331 NEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGLL 390
            +V+PV+ F++GGPRVGN  FK+R E+LGVKVLR+VN +D + K PG+  NE      + 
Sbjct: 329 GKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMK 388

Query: 391 LNGGGNNSYEHVGVELVLDFFNMQNP--------SCVHDLETYISLLRCPKEEAQDQDDD 450
           L GG    Y HVG  L LD  + ++P        S  H+LE  + LL          D  
Sbjct: 389 LAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL----------DGY 448

Query: 451 DDDGRRKIMINGGD--FINKAMEFL 454
              G+R ++ +G D   +NKA +FL
Sbjct: 449 HGKGQRFVLSSGRDPALVNKASDFL 459

BLAST of Lcy10g009840 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 5.2e-79
Identity = 162/344 (47.09%), Postives = 217/344 (63.08%), Query Frame = 0

Query: 91  WRQIHGCNDWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 150
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 151 KEVGLESSGYEVTKYIYATPPDINIPTIQNSP------PSCGRWIGYVAVSSDETSK-RL 210
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS DETS+ RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 211 GRRDIVVTFRGTVTNSEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 270
           GRRDI + +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 271 GLESCREQLLSEVSRLLNKYKKE---EVSITMAGHSMGSALALLLAYDIAELGLNRRTNN 330
              S REQ+L+EV RL+ ++  +   ++SIT+ GHS+G ALA+L AYDIAE+ LNR    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 331 EVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGLLL 390
           +V+PV+V ++GGPRVGN  F++R EELGVKV+R+VNV+D + K PG+  NE+     + +
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 391 NGGGNNSYEHVGVELVLDFFNMQ------NPSCVHDLETYISLL 418
             G    Y HVG EL LD  N        + S  H+LE  + LL
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL 446

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIN95.3e-13756.06Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5... [more]
Q9MA461.6e-13658.32Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 ... [more]
A2WY001.2e-11558.86Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica ... [more]
Q8S1D91.2e-11558.86Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonic... [more]
Q3EBR62.3e-7944.36Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3... [more]
Match NameE-valueIdentityDescription
A0A0A0L8A71.4e-24489.73Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE... [more]
A0A5A7VKE22.0e-24088.24Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577... [more]
A0A1S3AXV12.0e-24088.24galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973... [more]
A0A6J1GW193.1e-22083.64phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IP195.2e-22083.82phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_004148168.22.8e-24489.73galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical p... [more]
XP_008439078.14.2e-24088.24PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo] >KAA0067660.1... [more]
XP_038886126.12.4e-23587.84galactolipase DONGLE, chloroplastic-like [Benincasa hispida][more]
XP_022956251.16.3e-22083.64phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata][more]
XP_022979567.11.1e-21983.82phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G31690.13.8e-13856.06alpha/beta-Hydrolases superfamily protein [more]
AT1G05800.11.1e-13758.32alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.21.6e-8044.36alpha/beta-Hydrolases superfamily protein [more]
AT1G06800.13.6e-8044.68alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.15.2e-7947.09alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 268..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..54
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 21..471
NoneNo IPR availablePANTHERPTHR31403:SF4PHOSPHOLIPASE A1-IALPHA2, CHLOROPLASTICcoord: 21..471
NoneNo IPR availableCDDcd00519Lipase_3coord: 153..418
e-value: 1.32031E-50
score: 169.964
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 209..366
e-value: 2.1E-38
score: 131.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 90..475
e-value: 8.6E-100
score: 336.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 169..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g009840.1Lcy10g009840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0008970 phospholipase A1 activity