Lcy10g009660 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g009660
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr10: 36696966 .. 36697573 (+)
RNA-Seq ExpressionLcy10g009660
SyntenyLcy10g009660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACAACGCTTCATGCCTTATGGACCTGTAAGAAGGTAAAAAAGCACTGGGCCTGTTGGAGCCCAGTGCAACCTCTCCTTAGTACACATTACGCTGATGCCCTTGATCTGTTTTGTAGGTGTCAGAAGGTTCTTTCTACTGATGATTTGGCAACCTTCATGGCAGTCCTTTGGTCTATGTGGAACAGTAGAAATAAAGATGTTTTTCAGGACAACATGAACTATGTCCAACAGGAATACGACCCTATAGAATGGGCATCTGCTTACATATCAGACTACAAGGAGGCCCAAGCTAAGCATCATGGAGTCAATCGAGATCAAGAGCGGTTGAGAGATGCTGATGATTCATGTCTTACTGCGTGGATTCCGCCGCTCCAGAACCATTTCAAGTTGAATGTGGATGCAACTTTAGCTAATGGAGATATGGGATCAGGCATGATCATCAGAAATTCGAATGGAGAACCAATGGCAACCGCAGAGTATTTCAGGCGAACGAGCTGTTCAGTAGAGTGGGCAGAAGCAAAGGCTCTTGTAGACGACATTCAGTTAGCTGTTGATTCAGGATTATCACCAGTCTGGGCAGAGACGGATTCCAAAATTGTATAG

mRNA sequence

ATGAAACAACGCTTCATGCCTTATGGACCTGTAAGAAGGTGTCAGAAGGTTCTTTCTACTGATGATTTGGCAACCTTCATGGCAGTCCTTTGGTCTATGTGGAACAGTAGAAATAAAGATGTTTTTCAGGACAACATGAACTATGTCCAACAGGAATACGACCCTATAGAATGGGCATCTGCTTACATATCAGACTACAAGGAGGCCCAAGCTAAGCATCATGGAGTCAATCGAGATCAAGAGCGGTTGAGAGATGCTGATGATTCATGTCTTACTGCGTGGATTCCGCCGCTCCAGAACCATTTCAAGTTGAATGTGGATGCAACTTTAGCTAATGGAGATATGGGATCAGGCATGATCATCAGAAATTCGAATGGAGAACCAATGGCAACCGCAGAGTATTTCAGGCGAACGAGCTGTTCAGTAGAGTGGGCAGAAGCAAAGGCTCTTGTAGACGACATTCAGTTAGCTGTTGATTCAGGATTATCACCAGTCTGGGCAGAGACGGATTCCAAAATTGTATAG

Coding sequence (CDS)

ATGAAACAACGCTTCATGCCTTATGGACCTGTAAGAAGGTGTCAGAAGGTTCTTTCTACTGATGATTTGGCAACCTTCATGGCAGTCCTTTGGTCTATGTGGAACAGTAGAAATAAAGATGTTTTTCAGGACAACATGAACTATGTCCAACAGGAATACGACCCTATAGAATGGGCATCTGCTTACATATCAGACTACAAGGAGGCCCAAGCTAAGCATCATGGAGTCAATCGAGATCAAGAGCGGTTGAGAGATGCTGATGATTCATGTCTTACTGCGTGGATTCCGCCGCTCCAGAACCATTTCAAGTTGAATGTGGATGCAACTTTAGCTAATGGAGATATGGGATCAGGCATGATCATCAGAAATTCGAATGGAGAACCAATGGCAACCGCAGAGTATTTCAGGCGAACGAGCTGTTCAGTAGAGTGGGCAGAAGCAAAGGCTCTTGTAGACGACATTCAGTTAGCTGTTGATTCAGGATTATCACCAGTCTGGGCAGAGACGGATTCCAAAATTGTATAG

Protein sequence

MKQRFMPYGPVRRCQKVLSTDDLATFMAVLWSMWNSRNKDVFQDNMNYVQQEYDPIEWASAYISDYKEAQAKHHGVNRDQERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDMGSGMIIRNSNGEPMATAEYFRRTSCSVEWAEAKALVDDIQLAVDSGLSPVWAETDSKIV
Homology
BLAST of Lcy10g009660 vs. ExPASy TrEMBL
Match: A0A6J1CDQ4 (uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010533 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.2e-14
Identity = 55/169 (32.54%), Postives = 89/169 (52.66%), Query Frame = 0

Query: 11  VRRCQKVLSTDDLATFMAVLWSMWNSRNKDVFQDNMNYVQQEYDPIEWASAYISDYKEAQ 70
           +R  + +L+  D    +  LWS+WN RN  VF  N   V+ + D   W S YI+ +K   
Sbjct: 5   LRDWRDMLNWKDFEELVVFLWSLWNRRNAFVF--NKRRVEDD-DLAGWVSTYIATFKATN 64

Query: 71  AKHHGVNRD-----QERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDMGSGM---IIR 130
             H   N+D     Q+  +       T W P  +  FKL  DA+ ++ D  +G+   IIR
Sbjct: 65  TNHATANQDVSQSFQQSSQIHQAQNHTIWCPAEEGVFKLKTDASFSSIDFNAGLGVIIIR 124

Query: 131 NSNGEPMATAEYFRRTSCSVEWAEAKALVDDIQLAVDSGLSPVWAETDS 172
           +  G+ +A+A  +     SV+ AEA A V+ +++A+++G+SP+  ETDS
Sbjct: 125 DHRGQVLASATKYLEHVASVDDAEALAAVEGLRVAMETGISPILLETDS 170

BLAST of Lcy10g009660 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.0e-12
Identity = 51/164 (31.10%), Postives = 82/164 (50.00%), Query Frame = 0

Query: 4    RFMPYGPVRRCQKVLSTDDLATFMAVLWSMWNSRNKDVFQDNMNYV-QQEYDPIEWASAY 63
            +  P+  +R   + LS  D      V+W +WN RN   F D+   V +   + +EWA+ Y
Sbjct: 908  KLSPFLILRESHESLSKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKY 967

Query: 64   ISDYKEAQAKHHGVNRDQERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDM--GSGMI 123
              +++EA++     N    R+ +  +     W PP +  +K+N DA+    D   G G+I
Sbjct: 968  AMEFREAKS-----NPITGRVTNTAE---ILWQPPDEGIYKINTDASFLASDQHAGLGII 1027

Query: 124  IRNSNGEPMATAEYFRRTSCSVEWAEAKALVDDIQLAVDSGLSP 165
            I N  G+ MA A  +     SV+ AEA A V+ +QLA + G+ P
Sbjct: 1028 IHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHP 1063

BLAST of Lcy10g009660 vs. ExPASy TrEMBL
Match: A0A2N9H1N4 (RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS33433 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 7.7e-12
Identity = 53/166 (31.93%), Postives = 80/166 (48.19%), Query Frame = 0

Query: 11   VRRCQKVLSTDDLATFMAVLWSMWNSRNKDVFQDNMNYVQQEYDPIEWASAYISDYKEAQ 70
            + +C  +LST +L  F  + WS+W  RN+   Q +   V  +   ++ A   +S+++EAQ
Sbjct: 1338 LHKCFSLLSTSELQLFSMICWSIWYRRNRLRLQQS---VDNDLQLVQRARDSMSEFQEAQ 1397

Query: 71   AKHHGVNRDQERLRDADDSCLTAWIPPLQNHFKLNVDATLANGD--MGSGMIIRNSNGEP 130
                  +R+Q+  +    S    W PP Q  +K+N D  + +     G G+IIRN  GE 
Sbjct: 1398 ------DREQQLPQQPSSSATVKWNPPAQGRYKVNYDGAIFSDSNAAGIGVIIRNHQGEV 1457

Query: 131  MATAEYFRRTSCSVEWAEAKALVDDIQLAVDSGLSPVWAETDSKIV 175
            M +         SVE  EA A    IQ A D G + +  E DSK V
Sbjct: 1458 MGSLSQRFPFPHSVEAVEASAARSAIQFARDLGFTKIDLEGDSKTV 1494

BLAST of Lcy10g009660 vs. ExPASy TrEMBL
Match: A0A6J5XPA9 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS35751 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 8.5e-11
Identity = 53/149 (35.57%), Postives = 80/149 (53.69%), Query Frame = 0

Query: 29  VLWSMWNSRNKDVFQDNMNYVQQEYDP---IEWASAYISDYKEAQAKHHGVNRDQERLRD 88
           ++WS+W +R K +F+      QQ  +P   I+ A    +D+  +      VNRD ++  +
Sbjct: 94  LMWSIWKARYKAIFE------QQLPNPGLVIQGAKESWTDFLNSAPTLGAVNRDVDKESE 153

Query: 89  ADDSCLTAWIPPLQNHFKLNVDAT--LANGDMGSGMIIRNSNGEPMATAEYFRRTSCSVE 148
              S  T W  PL  + KLNVDA     +G  G G+IIRN +GE   + +    TS SV 
Sbjct: 154 VVRS--TLWQAPLTPYLKLNVDAAWFSNSGHTGIGIIIRNHHGE-FKSGKVCWTTSMSVV 213

Query: 149 WAEAKALVDDIQLAVDSGLSPVWAETDSK 173
            AEA+A+++ +  A D G S +W E+DSK
Sbjct: 214 DAEARAVIEGLSFAADQGYSHIWVESDSK 233

BLAST of Lcy10g009660 vs. ExPASy TrEMBL
Match: A0A6J1C467 (uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007775 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 8.5e-11
Identity = 48/156 (30.77%), Postives = 80/156 (51.28%), Query Frame = 0

Query: 21  DDLATFMAVLWSMWNSRNKDVFQDNMNYVQQEYDPIEWASAYISDYKEAQAKHHGVNRDQ 80
           ++L+ F   LW++WN+RN+ +F +   ++    + ++W + Y+  Y+ AQ          
Sbjct: 10  EELSVF---LWAIWNARNQSMFTNGHGFLL--VNMVDWVADYLQVYQSAQE-----GPSL 69

Query: 81  ERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDMGSGM--IIRNSNGEPMATAEYFRRT 140
             L D     ++AW PPL   FK+NVDA    G+  +G+  +IR+S    + +A  F   
Sbjct: 70  PLLLDGRGGRVSAWCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITH 129

Query: 141 SCSVEWAEAKALVDDIQLAVDSGLSPVWAETDSKIV 175
              V  AE  A  + + LA+++GL P   ETDS  V
Sbjct: 130 VSDVTLAECLAAQEGVCLAIEAGLIPFQIETDSSQV 155

BLAST of Lcy10g009660 vs. NCBI nr
Match: XP_022139684.1 (uncharacterized protein LOC111010533 [Momordica charantia])

HSP 1 Score: 89.0 bits (219), Expect = 4.5e-14
Identity = 55/169 (32.54%), Postives = 89/169 (52.66%), Query Frame = 0

Query: 11  VRRCQKVLSTDDLATFMAVLWSMWNSRNKDVFQDNMNYVQQEYDPIEWASAYISDYKEAQ 70
           +R  + +L+  D    +  LWS+WN RN  VF  N   V+ + D   W S YI+ +K   
Sbjct: 5   LRDWRDMLNWKDFEELVVFLWSLWNRRNAFVF--NKRRVEDD-DLAGWVSTYIATFKATN 64

Query: 71  AKHHGVNRD-----QERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDMGSGM---IIR 130
             H   N+D     Q+  +       T W P  +  FKL  DA+ ++ D  +G+   IIR
Sbjct: 65  TNHATANQDVSQSFQQSSQIHQAQNHTIWCPAEEGVFKLKTDASFSSIDFNAGLGVIIIR 124

Query: 131 NSNGEPMATAEYFRRTSCSVEWAEAKALVDDIQLAVDSGLSPVWAETDS 172
           +  G+ +A+A  +     SV+ AEA A V+ +++A+++G+SP+  ETDS
Sbjct: 125 DHRGQVLASATKYLEHVASVDDAEALAAVEGLRVAMETGISPILLETDS 170

BLAST of Lcy10g009660 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 82.4 bits (202), Expect = 4.2e-12
Identity = 51/164 (31.10%), Postives = 82/164 (50.00%), Query Frame = 0

Query: 4    RFMPYGPVRRCQKVLSTDDLATFMAVLWSMWNSRNKDVFQDNMNYV-QQEYDPIEWASAY 63
            +  P+  +R   + LS  D      V+W +WN RN   F D+   V +   + +EWA+ Y
Sbjct: 908  KLSPFLILRESHESLSKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKY 967

Query: 64   ISDYKEAQAKHHGVNRDQERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDM--GSGMI 123
              +++EA++     N    R+ +  +     W PP +  +K+N DA+    D   G G+I
Sbjct: 968  AMEFREAKS-----NPITGRVTNTAE---ILWQPPDEGIYKINTDASFLASDQHAGLGII 1027

Query: 124  IRNSNGEPMATAEYFRRTSCSVEWAEAKALVDDIQLAVDSGLSP 165
            I N  G+ MA A  +     SV+ AEA A V+ +QLA + G+ P
Sbjct: 1028 IHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHP 1063

BLAST of Lcy10g009660 vs. NCBI nr
Match: XP_021724891.1 (uncharacterized protein LOC110692199 [Chenopodium quinoa])

HSP 1 Score: 77.8 bits (190), Expect = 1.0e-10
Identity = 52/163 (31.90%), Postives = 82/163 (50.31%), Query Frame = 0

Query: 12  RRCQKVLSTDDLATFMAVLWSMWNSRNKDVFQDNMNYVQQEYDPIEWA---SAYISDYKE 71
           +R  +  S  D+ TF A+ W+MW SRNK +F+++       +DPI  A   S Y+SD++ 
Sbjct: 63  KRVTEHYSHHDVYTFSALAWAMWTSRNKALFENS------PHDPILLATGFSKYVSDFQV 122

Query: 72  AQAKHHGVNRDQERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDMGSGMIIRNSNGEP 131
             AK  G  R    + +      + W  P     K+NVDA   +  +G G++ RNS+G+ 
Sbjct: 123 YSAKVFGGRRGNGGISN------STWKKPPAGTLKINVDAATFDDAVGLGVVARNSHGDI 182

Query: 132 MATAEYFRRTSCSVEWAEAKALVDDIQLAVDSGLSPVWAETDS 172
           + T     R       AEA A+   ++LA+  G   VW E+D+
Sbjct: 183 VFTGARRWRGRWDSCIAEAAAIHFGVELAIRFGYRDVWCESDA 213

BLAST of Lcy10g009660 vs. NCBI nr
Match: XP_022135942.1 (uncharacterized protein LOC111007775 [Momordica charantia])

HSP 1 Score: 77.0 bits (188), Expect = 1.8e-10
Identity = 48/156 (30.77%), Postives = 80/156 (51.28%), Query Frame = 0

Query: 21  DDLATFMAVLWSMWNSRNKDVFQDNMNYVQQEYDPIEWASAYISDYKEAQAKHHGVNRDQ 80
           ++L+ F   LW++WN+RN+ +F +   ++    + ++W + Y+  Y+ AQ          
Sbjct: 10  EELSVF---LWAIWNARNQSMFTNGHGFLL--VNMVDWVADYLQVYQSAQE-----GPSL 69

Query: 81  ERLRDADDSCLTAWIPPLQNHFKLNVDATLANGDMGSGM--IIRNSNGEPMATAEYFRRT 140
             L D     ++AW PPL   FK+NVDA    G+  +G+  +IR+S    + +A  F   
Sbjct: 70  PLLLDGRGGRVSAWCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITH 129

Query: 141 SCSVEWAEAKALVDDIQLAVDSGLSPVWAETDSKIV 175
              V  AE  A  + + LA+++GL P   ETDS  V
Sbjct: 130 VSDVTLAECLAAQEGVCLAIEAGLIPFQIETDSSQV 155

BLAST of Lcy10g009660 vs. NCBI nr
Match: CAB4312998.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 77.0 bits (188), Expect = 1.8e-10
Identity = 53/149 (35.57%), Postives = 80/149 (53.69%), Query Frame = 0

Query: 29  VLWSMWNSRNKDVFQDNMNYVQQEYDP---IEWASAYISDYKEAQAKHHGVNRDQERLRD 88
           ++WS+W +R K +F+      QQ  +P   I+ A    +D+  +      VNRD ++  +
Sbjct: 94  LMWSIWKARYKAIFE------QQLPNPGLVIQGAKESWTDFLNSAPTLGAVNRDVDKESE 153

Query: 89  ADDSCLTAWIPPLQNHFKLNVDAT--LANGDMGSGMIIRNSNGEPMATAEYFRRTSCSVE 148
              S  T W  PL  + KLNVDA     +G  G G+IIRN +GE   + +    TS SV 
Sbjct: 154 VVRS--TLWQAPLTPYLKLNVDAAWFSNSGHTGIGIIIRNHHGE-FKSGKVCWTTSMSVV 213

Query: 149 WAEAKALVDDIQLAVDSGLSPVWAETDSK 173
            AEA+A+++ +  A D G S +W E+DSK
Sbjct: 214 DAEARAVIEGLSFAADQGYSHIWVESDSK 233

BLAST of Lcy10g009660 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.7e-06
Identity = 47/158 (29.75%), Postives = 68/158 (43.04%), Query Frame = 0

Query: 21  DDLATFMA--VLWSMWNSRNKDVFQDNMNYVQQEYDPIEWASAYISDYKEAQAKHHGVNR 80
           D +  FM   ++W +W S N  VF       Q     +E A   ++D KE    +   N 
Sbjct: 37  DRVTRFMPFWLMWRIWKSGNDLVFNHTRTKFQ---TTVEMA---LNDTKE-WLDNTMTNE 96

Query: 81  DQERLRDADDSCLTAWIPPLQNHFKLNVDAT--LANGDMGSGMIIRNSNGEPMATAEYFR 140
            Q   R+AD S  T W PP ++  K N DA+    N   G G I+RNS G  +       
Sbjct: 97  QQNGNRNADPSRNTKWSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKF 156

Query: 141 RTSCSVEWAEAKALVDDIQLAVDSGLSPVWAETDSKIV 175
           +   + E AE   L+  IQ +   G   V  E D++ +
Sbjct: 157 QGRMTTEEAECSTLIWAIQASYGFGHKKVIFEGDNQTI 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CDQ42.2e-1432.54uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1DAR42.0e-1231.10uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A2N9H1N47.7e-1231.93RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS33433 ... [more]
A0A6J5XPA98.5e-1135.57Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS35751 PE=... [more]
A0A6J1C4678.5e-1130.77uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
Match NameE-valueIdentityDescription
XP_022139684.14.5e-1432.54uncharacterized protein LOC111010533 [Momordica charantia][more]
XP_022150918.14.2e-1231.10uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_021724891.11.0e-1031.90uncharacterized protein LOC110692199 [Chenopodium quinoa][more]
XP_022135942.11.8e-1030.77uncharacterized protein LOC111007775 [Momordica charantia][more]
CAB4312998.11.8e-1035.57unnamed protein product [Prunus armeniaca][more]
Match NameE-valueIdentityDescription
AT5G65005.13.7e-0629.75Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 106..174
e-value: 8.1E-10
score: 38.6
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 100..174
e-value: 4.2E-5
score: 25.6
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 90..174
NoneNo IPR availablePANTHERPTHR47074:SF26SUBFAMILY NOT NAMEDcoord: 90..174
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 104..174
e-value: 9.08991E-10
score: 51.9312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g009660.1Lcy10g009660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity