Lcy10g009260 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g009260
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptiondisease resistance protein RGA2-like
LocationChr10: 35739487 .. 35740966 (-)
RNA-Seq ExpressionLcy10g009260
SyntenyLcy10g009260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTCTTTCCCAACTCCTGCAAATTTGCAGAGGCTCACGAAACAATAATGGCGGAATTCTTGTGGACTTTTGCTGTGCAAGAAGTGTTGAAGAAGACAGTGAAACTTGCAGCAGAGCAGATTGGTCTGGCGTGGGGTTTCAACGAAGAGCTGATAAAGCTGAGGGACTCTTTACAATCTGTGGAAGCCATCTTATGCGATAATGACAGAATAAAGTCACAGTATCCTTCCATGAAGCCATGGGTGGAGAAGCTTCAAGATATTGTTTTCGAAGCCGATGATCTACTGGATGAACTCGCTTATGAAGATCTCCGACTCAAGGTGGAAACTGGAAAAAATGTGATGGTACGTAATTTCGTTTCATACTCCAAAAACCCTCTTGTTTTTCGTCTCAAAATGGCCAATAAAATTATGGTCGTCGCTAAAATGTTACATGAGCATTATTGTGCCACGAGTGCTATGGGACTTGTTGCTGTTACAGCCAGAGAATTTGAACCTGATTTTTGCCAGATTCAAGAAACAGACTCTTTTCTAGAAGAGACTGGAGTTATCGGAAGAGAAATTGAAATATTGGAAATAGTCGACAAGTTGATTGACCTTAGCAATGAAGAAGCACTCTCTGTTCTTTCCATTGTTGGTATGGGTGGATTAGGGAAAACAGCTTTGGCTAAGGTAATCTTCAATCATGAAGTGGTAAGGAAATTTTTTGATAAAACTATATGGGTTTGTGTGTCTGAACCCTTTGTTATAAAAAAGATTCTAAAAGCAATTTTGGAAACCCTTAATGCTAATTTAAGTGGCCTAGATAGCAAGGAAGCCTTACTTCAAGAGCTCCAAAAATTGATGAATGACAAAAAGTACTTTTTAGTGTTGGATGATGTTTGGACTGAAAACTCTAACTTATGGAATGAGTTCAAGACTTGTTTGCTAAAGATTACTAAAAAATCTGGGAGTGTTATCCTTGTGACTACAAGGAGTGATGGAGTTGCAGAAACAATGGAAACACATCCCAGATATCATTTGAGAAATGTGCATTTGCAAATGAATTGCTAATAGTTACAAAGTTGGATGTAGTTCTTCAGGATGAGCTTGTTAAAAAATTTGGTGGCATACCATTGGTTGTAAAAGTGTTAGGAGGAATGGTGAAATTAGAAAAGAATCAAGAGAGATTGCAATCAATCTTGGAAGGTTTAATTACAATTCCATTGCAGGATGAAAGTTTTGTTTTATCCACAATAAAATTAAGTGTGGATCGTCTACCATCATCTTCATTAAAACAGTGTTTTGCCTATTGTTCGAATTATTCCAAAGACTTTGTGTTCCCGAAAGAAGAACTTATTCAAATTTGGATGGCAAAAGGGTTTATTCAAACACCCAAGGAAAGCAATGTAACGATGGAAGATATTGGAGACAAGTACTTCAACTTTTTGTTGTCACGTTCCTTGTTTGATGTTGTCAAGGATGATAGAGGGAGAATTATA

mRNA sequence

TTCTCTTTCCCAACTCCTGCAAATTTGCAGAGGCTCACGAAACAATAATGGCGGAATTCTTGTGGACTTTTGCTGTGCAAGAAGTGTTGAAGAAGACAGTGAAACTTGCAGCAGAGCAGATTGGTCTGGCGTGGGGTTTCAACGAAGAGCTGATAAAGCTGAGGGACTCTTTACAATCTGTGGAAGCCATCTTATGCGATAATGACAGAATAAAGTCACAGTATCCTTCCATGAAGCCATGGGTGGAGAAGCTTCAAGATATTGTTTTCGAAGCCGATGATCTACTGGATGAACTCGCTTATGAAGATCTCCGACTCAAGGTGGAAACTGGAAAAAATGTGATGGTACGTAATTTCGTTTCATACTCCAAAAACCCTCTTGTTTTTCGTCTCAAAATGGCCAATAAAATTATGGTCGTCGCTAAAATGTTACATGAGCATTATTGTGCCACGAGTGCTATGGGACTTGTTGCTGTTACAGCCAGAGAATTTGAACCTGATTTTTGCCAGATTCAAGAAACAGACTCTTTTCTAGAAGAGACTGGAGTTATCGGAAGAGAAATTGAAATATTGGAAATAGTCGACAAGTTGATTGACCTTAGCAATGAAGAAGCACTCTCTGTTCTTTCCATTGTTGGTATGGGTGGATTAGGGAAAACAGCTTTGGCTAAGGTAATCTTCAATCATGAAGTGGTAAGGAAATTTTTTGATAAAACTATATGGGTTTGTGTGTCTGAACCCTTTGTTATAAAAAAGATTCTAAAAGCAATTTTGGAAACCCTTAATGCTAATTTAAGTGGCCTAGATAGCAAGGAAGCCTTACTTCAAGAGCTCCAAAAATTGATGAATGACAAAAAGTACTTTTTAGTGTTGGATGATGTTTGGACTGAAAACTCTAACTTATGGAATGAGTTCAAGACTTGTTTGCTAAAGATTACTAAAAAATCTGGGAGTGTTATCCTTGTGACTACAAGGAGTGATGGAGTTGCAGAAACAATGGAAACACATCCCAGATATCATTTGAGAAATGTGCATTTGCAAATGAATTGCTAATAGTTACAAAGTTGGATGTAGTTCTTCAGGATGAGCTTGTTAAAAAATTTGGTGGCATACCATTGGTTGTAAAAGTGTTAGGAGGAATGGTGAAATTAGAAAAGAATCAAGAGAGATTGCAATCAATCTTGGAAGGTTTAATTACAATTCCATTGCAGGATGAAAGTTTTGTTTTATCCACAATAAAATTAAGTGTGGATCGTCTACCATCATCTTCATTAAAACAGTGTTTTGCCTATTGTTCGAATTATTCCAAAGACTTTGTGTTCCCGAAAGAAGAACTTATTCAAATTTGGATGGCAAAAGGGTTTATTCAAACACCCAAGGAAAGCAATGTAACGATGGAAGATATTGGAGACAAGTACTTCAACTTTTTGTTGTCACGTTCCTTGTTTGATGTTGTCAAGGATGATAGAGGGAGAATTATA

Coding sequence (CDS)

ATGGCGGAATTCTTGTGGACTTTTGCTGTGCAAGAAGTGTTGAAGAAGACAGTGAAACTTGCAGCAGAGCAGATTGGTCTGGCGTGGGGTTTCAACGAAGAGCTGATAAAGCTGAGGGACTCTTTACAATCTGTGGAAGCCATCTTATGCGATAATGACAGAATAAAGTCACAGTATCCTTCCATGAAGCCATGGGTGGAGAAGCTTCAAGATATTGTTTTCGAAGCCGATGATCTACTGGATGAACTCGCTTATGAAGATCTCCGACTCAAGGTGGAAACTGGAAAAAATGTGATGGTACGTAATTTCGTTTCATACTCCAAAAACCCTCTTGTTTTTCGTCTCAAAATGGCCAATAAAATTATGGTCGTCGCTAAAATGTTACATGAGCATTATTGTGCCACGAGTGCTATGGGACTTGTTGCTGTTACAGCCAGAGAATTTGAACCTGATTTTTGCCAGATTCAAGAAACAGACTCTTTTCTAGAAGAGACTGGAGTTATCGGAAGAGAAATTGAAATATTGGAAATAGTCGACAAGTTGATTGACCTTAGCAATGAAGAAGCACTCTCTGTTCTTTCCATTGTTGGTATGGGTGGATTAGGGAAAACAGCTTTGGCTAAGGTAATCTTCAATCATGAAGTGGTAAGGAAATTTTTTGATAAAACTATATGGGTTTGTGTGTCTGAACCCTTTGTTATAAAAAAGATTCTAAAAGCAATTTTGGAAACCCTTAATGCTAATTTAAGTGGCCTAGATAGCAAGGAAGCCTTACTTCAAGAGCTCCAAAAATTGATGAATGACAAAAAGTACTTTTTAGTGTTGGATGATGTTTGGACTGAAAACTCTAACTTATGGAATGAGTTCAAGACTTGTTTGCTAAAGATTACTAAAAAATCTGGGAGTGTTATCCTTGTGACTACAAGGAGTGATGGAGTTGCAGAAACAATGGAAACACATCCCAGATATCATTTGAGAAATGTGCATTTGCAAATGAATTGCTAA

Protein sequence

MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANKIMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDKLIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKAILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKSGSVILVTTRSDGVAETMETHPRYHLRNVHLQMNC
Homology
BLAST of Lcy10g009260 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 1.5e-37
Identity = 110/312 (35.26%), Postives = 169/312 (54.17%), Query Frame = 0

Query: 24  QIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKLQDIVFEADDLLDEL 83
           ++GL +GF +E  KL      ++A+L D    + +Y ++K W++KL    +E DD+LD+ 
Sbjct: 20  ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79

Query: 84  AYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANKIMVVAKMLHEHYCATSAMGLVAV 143
             E  R K    + V+ R    Y    + F  K+  ++  + + L            +A 
Sbjct: 80  KTEAARFK----QAVLGR----YHPRTITFCYKVGKRMKEMMEKL----------DAIAE 139

Query: 144 TAREFEPDFCQIQ------ETDSFLEETGVIGREIEILEIVDKLI-DLSNEEALSVLSIV 203
             R F  D   I+      +T   L E  V GRE E  EIV  LI ++S  E + VL I+
Sbjct: 140 ERRNFHLDERIIERQAARRQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPIL 199

Query: 204 GMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKAILETLNANLSGLDSKE 263
           GMGGLGKT LA+++FN + + + F+  IWVCVS+ F  K+++KAI+E++     G     
Sbjct: 200 GMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLA 259

Query: 264 ALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKSGSVILVTTRSDGVAET 323
            L ++LQ+L+N K+YFLVLDDVW E+   W+  +  +LKI   SG+ IL+TTR + +   
Sbjct: 260 PLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLR-AVLKI-GASGASILITTRLEKIGSI 311

Query: 324 METHPRYHLRNV 329
           M T   Y L N+
Sbjct: 320 MGTLQLYQLSNL 311

BLAST of Lcy10g009260 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 5.4e-35
Identity = 111/328 (33.84%), Postives = 176/328 (53.66%), Query Frame = 0

Query: 10  VQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKL 69
           +Q VL         ++ L +GF +E  +L     +++A+L D    +     ++ W++KL
Sbjct: 6   IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65

Query: 70  QDIVFEADDLLDELAYEDLR-LKVETGKNVMVRNFVSYSKNPLVFRLKMANKIMVVAKML 129
               +E DD+LDE   +  R L+ E G+         Y    + FR K+  ++  V K L
Sbjct: 66  NAATYEVDDILDEYKTKATRFLQSEYGR---------YHPKVIPFRHKVGKRMDQVMKKL 125

Query: 130 HEHYCATSAMGLVAVTAREFEPDFCQIQ------ETDSFLEETGVIGREIEILEIVDKLI 189
           +           +A   ++F      I+      ET S L E  V GR+ E  EIV  LI
Sbjct: 126 N----------AIAEERKKFHLQEKIIERQAATRETGSVLTEPQVYGRDKEKDEIVKILI 185

Query: 190 D-LSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKAI 249
           +  S+ + LSVL I+GMGGLGKT L++++FN + V + F   IW+C+S+ F  K+++KAI
Sbjct: 186 NTASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAI 245

Query: 250 LETLNA-NLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 309
           +E++   +LS +D    L ++LQ+L+N K+YFLVLDDVW E+ + W   +  +LK+   S
Sbjct: 246 VESIEGKSLSDMD-LAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLR-AVLKV-GAS 305

Query: 310 GSVILVTTRSDGVAETMETHPRYHLRNV 329
           G+ +L TTR + V   M T   Y L N+
Sbjct: 306 GAFVLTTTRLEKVGSIMGTLQPYELSNL 311

BLAST of Lcy10g009260 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.9e-34
Identity = 107/327 (32.72%), Postives = 166/327 (50.76%), Query Frame = 0

Query: 10  VQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKL 69
           +Q +L         ++ L +GF +E  +L     +++A+L D    +     ++ W++KL
Sbjct: 6   IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65

Query: 70  QDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANKIMVVAKMLH 129
               +E DD+LDE   +  R             +  Y    + FR K+  ++  V K L 
Sbjct: 66  NAATYEVDDILDEYKTKATRFSQS--------EYGRYHPKVIPFRHKVGKRMDQVMKKLK 125

Query: 130 EHYCATSAMGLVAVTAREFEPDFCQIQ------ETDSFLEETGVIGREIEILEIVDKLI- 189
                      +A   + F      ++      ET S L E  V GR+ E  EIV  LI 
Sbjct: 126 ----------AIAEERKNFHLHEKIVERQAVRRETGSVLTEPQVYGRDKEKDEIVKILIN 185

Query: 190 DLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKAIL 249
           ++S+ + LSVL I+GMGGLGKT LA+++FN + V + F   IW+CVSE F  K+++KAI+
Sbjct: 186 NVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIV 245

Query: 250 ETLNAN-LSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKSG 309
           E++    L G      L ++LQ+L+N K+Y LVLDDVW E+   W   +  +LK+   SG
Sbjct: 246 ESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLR-AVLKV-GASG 305

Query: 310 SVILVTTRSDGVAETMETHPRYHLRNV 329
           + +L TTR + V   M T   Y L N+
Sbjct: 306 ASVLTTTRLEKVGSIMGTLQPYELSNL 312

BLAST of Lcy10g009260 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.9e-33
Identity = 103/328 (31.40%), Postives = 164/328 (50.00%), Query Frame = 0

Query: 10  VQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKL 69
           +Q +L+       +++ L +GF +E  KL     +++A+L D    + +  +++ W++KL
Sbjct: 6   LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65

Query: 70  QDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANKIMVVAKMLH 129
               +E DD+L E   E +R +              Y    + FR K+  ++  + + L 
Sbjct: 66  NSAAYEVDDILGECKNEAIRFEQS--------RLGFYHPGIINFRHKIGRRMKEIMEKLD 125

Query: 130 E--------HYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDKL 189
                    H+       L  +T R+        +ET   L E  V GR+ E  EIV  L
Sbjct: 126 AISEERRKFHF-------LEKITERQAA---AATRETGFVLTEPKVYGRDKEEDEIVKIL 185

Query: 190 IDLSN-EEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 249
           I+  N  E L V  I+GMGGLGKT LA++IFN E V K F+  IWVCVS+ F  K+++K 
Sbjct: 186 INNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKT 245

Query: 250 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 309
           I+  +  +   ++   +  ++LQ+L+N K+Y LVLDDVW ++   W + +  L       
Sbjct: 246 IIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGAR 305

Query: 310 GSVILVTTRSDGVAETMETHPRYHLRNV 329
           G+ IL TTR + V   M T   YHL N+
Sbjct: 306 GASILATTRLEKVGSIMGTLQPYHLSNL 313

BLAST of Lcy10g009260 vs. ExPASy Swiss-Prot
Match: P0C8S1 (Probable disease resistance RPP8-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RPP8L2 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 96/320 (30.00%), Postives = 173/320 (54.06%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAE + +F V+    K  +L + +     G +E++  L+  L  ++++L D D  K++  
Sbjct: 1   MAEAVVSFGVE----KLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
            ++ ++E ++DIV++ADD+++     +LR K E G    VR    +    LV R K A+ 
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELRGK-EKGIKKQVRTLACF----LVDRRKFASD 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVT-----AREFEPDFCQIQETDSFLEETGVIGREIEIL 180
           I  + K + E      ++G+  +      +   +    +I++T S   E+ ++G +  + 
Sbjct: 121 IEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSVE 180

Query: 181 EIVDKLIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIK 240
           E+VD L++    +++ V+S+ GMGG+GKT LA+ +F+H++VR+ FD   WVCVS+ F  K
Sbjct: 181 ELVDHLVE---NDSVQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTRK 240

Query: 241 KILKAILETLNANLSGLDSKE--ALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCL 300
            + + IL+ L     G+   +   L  EL +L+   +Y LVLDDVW E    W+  K   
Sbjct: 241 DVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAV- 300

Query: 301 LKITKKSGSVILVTTRSDGV 314
                K G  +L+T+R++G+
Sbjct: 301 --FPHKRGWKMLLTSRNEGL 303

BLAST of Lcy10g009260 vs. ExPASy TrEMBL
Match: A0A6J1CUL2 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014727 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.9e-129
Identity = 238/328 (72.56%), Postives = 278/328 (84.76%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LK+ VKLAAEQ  LAWGFN+EL+KLRDSL  VEAIL D DRIK ++ 
Sbjct: 1   MAEFLWTFAAQELLKQAVKLAAEQTALAWGFNKELLKLRDSLLMVEAILRDVDRIKGEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKLQ+IVFEAD LLD+LAYE LR KVE G   MV NFVS SKNPL FRLKMANK
Sbjct: 61  AVKLWVEKLQEIVFEADVLLDDLAYEHLRRKVEAGNEAMVSNFVSISKNPLAFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +A+ML +HY + +A+GLVAVT +E EPDF QIQETDSFL+E GVIGREIE+ EIV++
Sbjct: 121 IRAIARMLDKHYSSAAAVGLVAVTPKEIEPDFSQIQETDSFLDEFGVIGREIEVSEIVNR 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
           LID S+ +ALSVL IVGMGGLGKTAL KV+FNHE ++  FD+TIWVCVSEPFVIKKIL+A
Sbjct: 181 LIDFSHRDALSVLPIVGMGGLGKTALMKVVFNHEFIKGNFDRTIWVCVSEPFVIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN N  GLDSKEALLQELQKL+NDK+ FLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNPNFGGLDSKEALLQELQKLLNDKRCFLVLDDVWNENLTLWNELKACLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHLRNV 329
           G+ ++VTTRSD VAE METHPR+HL+++
Sbjct: 301 GNTVVVTTRSDKVAEVMETHPRHHLKSL 328

BLAST of Lcy10g009260 vs. ExPASy TrEMBL
Match: A0A5D3CZ17 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001180 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 6.6e-129
Identity = 240/325 (73.85%), Postives = 277/325 (85.23%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LKKTVKLAAEQIGLAWGFN EL  LRDSL  VEAIL D DRIK+++ 
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKL+ IVFE D LLDELAYEDLR KVE+ K  +V NF+S+SK PLVFRLKMANK
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +AKML +HY A S +GLVA+ +++ EPDF QIQETDSFL+E GVIGRE E+LEIV+ 
Sbjct: 121 IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
            +DLS  E LSVL IVGMGGLGKTALAKVIFNHE+++  FD+T+WVCVSEPF+IKKIL+A
Sbjct: 181 SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN+N  GLDSKEALLQELQKL+NDKKYFLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHL 326
           G+V++VTTRSD VAE METH RYHL
Sbjct: 301 GNVVVVTTRSDRVAEIMETHSRYHL 325

BLAST of Lcy10g009260 vs. ExPASy TrEMBL
Match: A0A5A7TSS6 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003620 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 6.6e-129
Identity = 240/325 (73.85%), Postives = 277/325 (85.23%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LKKTVKLAAEQIGLAWGFN EL  LRDSL  VEAIL D DRIK+++ 
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKL+ IVFE D LLDELAYEDLR KVE+ K  +V NF+S+SK PLVFRLKMANK
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +AKML +HY A S +GLVA+ +++ EPDF QIQETDSFL+E GVIGRE E+LEIV+ 
Sbjct: 121 IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
            +DLS  E LSVL IVGMGGLGKTALAKVIFNHE+++  FD+T+WVCVSEPF+IKKIL+A
Sbjct: 181 SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN+N  GLDSKEALLQELQKL+NDKKYFLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHL 326
           G+V++VTTRSD VAE METH RYHL
Sbjct: 301 GNVVVVTTRSDRVAEIMETHSRYHL 325

BLAST of Lcy10g009260 vs. ExPASy TrEMBL
Match: A0A1S3BXI2 (putative disease resistance protein RGA3 OS=Cucumis melo OX=3656 GN=LOC103494528 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 6.6e-129
Identity = 240/325 (73.85%), Postives = 277/325 (85.23%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LKKTVKLAAEQIGLAWGFN EL  LRDSL  VEAIL D DRIK+++ 
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKL+ IVFE D LLDELAYEDLR KVE+ K  +V NF+S+SK PLVFRLKMANK
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +AKML +HY A S +GLVA+ +++ EPDF QIQETDSFL+E GVIGRE E+LEIV+ 
Sbjct: 121 IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
            +DLS  E LSVL IVGMGGLGKTALAKVIFNHE+++  FD+T+WVCVSEPF+IKKIL+A
Sbjct: 181 SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN+N  GLDSKEALLQELQKL+NDKKYFLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHL 326
           G+V++VTTRSD VAE METH RYHL
Sbjct: 301 GNVVVVTTRSDRVAEIMETHSRYHL 325

BLAST of Lcy10g009260 vs. ExPASy TrEMBL
Match: A0A0A0KW56 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015840 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.6e-127
Identity = 238/325 (73.23%), Postives = 275/325 (84.62%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LKKTVKLAAEQIGLAWGFN EL  LRDSL  VEAIL D DRIK+++ 
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKL+ I+FE D LLDELAYEDLR KVE  K +MV NF+S+SK PLVFRLKMANK
Sbjct: 61  AVKLWVEKLEAIIFEVDVLLDELAYEDLRRKVEPQKEMMVSNFISFSKTPLVFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +AKML  HY A S +GLVA+ +++ EPDF QIQETDSFL+E GVIGRE E+LEIV+ 
Sbjct: 121 IKNIAKMLERHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEYGVIGRESEVLEIVNV 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
            +DLS  E LSVL IVGMGGLGKTALAKVIFNHE+++  FD+ +WVCVSEPF+IKKIL+A
Sbjct: 181 SVDLSYRENLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRAVWVCVSEPFLIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN++  GLDSKEALLQELQKL+NDKKYFLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNSHFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHL 326
           G+V++VTTRSD VAE METH RYHL
Sbjct: 301 GNVVVVTTRSDRVAEIMETHSRYHL 325

BLAST of Lcy10g009260 vs. NCBI nr
Match: XP_022145224.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 471.1 bits (1211), Expect = 8.0e-129
Identity = 238/328 (72.56%), Postives = 278/328 (84.76%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LK+ VKLAAEQ  LAWGFN+EL+KLRDSL  VEAIL D DRIK ++ 
Sbjct: 1   MAEFLWTFAAQELLKQAVKLAAEQTALAWGFNKELLKLRDSLLMVEAILRDVDRIKGEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKLQ+IVFEAD LLD+LAYE LR KVE G   MV NFVS SKNPL FRLKMANK
Sbjct: 61  AVKLWVEKLQEIVFEADVLLDDLAYEHLRRKVEAGNEAMVSNFVSISKNPLAFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +A+ML +HY + +A+GLVAVT +E EPDF QIQETDSFL+E GVIGREIE+ EIV++
Sbjct: 121 IRAIARMLDKHYSSAAAVGLVAVTPKEIEPDFSQIQETDSFLDEFGVIGREIEVSEIVNR 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
           LID S+ +ALSVL IVGMGGLGKTAL KV+FNHE ++  FD+TIWVCVSEPFVIKKIL+A
Sbjct: 181 LIDFSHRDALSVLPIVGMGGLGKTALMKVVFNHEFIKGNFDRTIWVCVSEPFVIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN N  GLDSKEALLQELQKL+NDK+ FLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNPNFGGLDSKEALLQELQKLLNDKRCFLVLDDVWNENLTLWNELKACLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHLRNV 329
           G+ ++VTTRSD VAE METHPR+HL+++
Sbjct: 301 GNTVVVTTRSDKVAEVMETHPRHHLKSL 328

BLAST of Lcy10g009260 vs. NCBI nr
Match: XP_008453966.1 (PREDICTED: putative disease resistance protein RGA3 [Cucumis melo] >TYK16977.1 putative disease resistance protein RGA3 [Cucumis melo var. makuwa])

HSP 1 Score: 470.3 bits (1209), Expect = 1.4e-128
Identity = 240/325 (73.85%), Postives = 277/325 (85.23%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LKKTVKLAAEQIGLAWGFN EL  LRDSL  VEAIL D DRIK+++ 
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKL+ IVFE D LLDELAYEDLR KVE+ K  +V NF+S+SK PLVFRLKMANK
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +AKML +HY A S +GLVA+ +++ EPDF QIQETDSFL+E GVIGRE E+LEIV+ 
Sbjct: 121 IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
            +DLS  E LSVL IVGMGGLGKTALAKVIFNHE+++  FD+T+WVCVSEPF+IKKIL+A
Sbjct: 181 SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN+N  GLDSKEALLQELQKL+NDKKYFLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHL 326
           G+V++VTTRSD VAE METH RYHL
Sbjct: 301 GNVVVVTTRSDRVAEIMETHSRYHL 325

BLAST of Lcy10g009260 vs. NCBI nr
Match: KAA0044611.1 (putative disease resistance protein RGA3 [Cucumis melo var. makuwa])

HSP 1 Score: 470.3 bits (1209), Expect = 1.4e-128
Identity = 240/325 (73.85%), Postives = 277/325 (85.23%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LKKTVKLAAEQIGLAWGFN EL  LRDSL  VEAIL D DRIK+++ 
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKL+ IVFE D LLDELAYEDLR KVE+ K  +V NF+S+SK PLVFRLKMANK
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +AKML +HY A S +GLVA+ +++ EPDF QIQETDSFL+E GVIGRE E+LEIV+ 
Sbjct: 121 IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
            +DLS  E LSVL IVGMGGLGKTALAKVIFNHE+++  FD+T+WVCVSEPF+IKKIL+A
Sbjct: 181 SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN+N  GLDSKEALLQELQKL+NDKKYFLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHL 326
           G+V++VTTRSD VAE METH RYHL
Sbjct: 301 GNVVVVTTRSDRVAEIMETHSRYHL 325

BLAST of Lcy10g009260 vs. NCBI nr
Match: XP_038874765.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 466.1 bits (1198), Expect = 2.6e-127
Identity = 235/331 (71.00%), Postives = 283/331 (85.50%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           M EFLWTFAVQE+LKKTVKLAAEQIGL WGF ++L K+RDSL  V+AIL D DRIKS+YP
Sbjct: 1   MVEFLWTFAVQEILKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSEYP 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNV--MVRNFVSYSKNPLVFRLKMA 120
            +KPWVEKL+DIVFEAD LLDELAYEDLRLKVE GK++   V NF+S S+NPL FRLKMA
Sbjct: 61  DVKPWVEKLEDIVFEADVLLDELAYEDLRLKVEIGKDLGKKVHNFISASENPLFFRLKMA 120

Query: 121 NKIMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIV 180
           N+I  +++ML EHY A S +GLVA+T++E EPD  Q+ ETDSFL+E GVIGR+ E+LEIV
Sbjct: 121 NRIKAISRMLQEHYAAASVVGLVAITSKEIEPDLSQVHETDSFLDEFGVIGRDEEVLEIV 180

Query: 181 DKLIDLSN-EEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKI 240
           +KLI+LSN E  LSVL IVGMGGLGKTALAKVIFNHEV+++ FD+ IWVCVS+PFVI KI
Sbjct: 181 NKLIELSNGEMPLSVLPIVGMGGLGKTALAKVIFNHEVIKENFDRFIWVCVSDPFVINKI 240

Query: 241 LKAILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKIT 300
           L+AILETLN N  GL+SKEALLQE+QKL++DK YFLVLDDVW EN +LWNE K CLLK+T
Sbjct: 241 LRAILETLNPNSGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKVT 300

Query: 301 KKSGSVILVTTRSDGVAETMETHPRYHLRNV 329
           +K G+V++VT+RSD VA+ METHPR++L+ +
Sbjct: 301 RKFGNVVIVTSRSDEVAKIMETHPRHYLKKL 331

BLAST of Lcy10g009260 vs. NCBI nr
Match: XP_004152292.1 (putative disease resistance protein RGA3 [Cucumis sativus] >KGN53094.1 hypothetical protein Csa_014593 [Cucumis sativus])

HSP 1 Score: 465.7 bits (1197), Expect = 3.4e-127
Identity = 238/325 (73.23%), Postives = 275/325 (84.62%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAEFLWTFA QE+LKKTVKLAAEQIGLAWGFN EL  LRDSL  VEAIL D DRIK+++ 
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
           ++K WVEKL+ I+FE D LLDELAYEDLR KVE  K +MV NF+S+SK PLVFRLKMANK
Sbjct: 61  AVKLWVEKLEAIIFEVDVLLDELAYEDLRRKVEPQKEMMVSNFISFSKTPLVFRLKMANK 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDK 180
           I  +AKML  HY A S +GLVA+ +++ EPDF QIQETDSFL+E GVIGRE E+LEIV+ 
Sbjct: 121 IKNIAKMLERHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEYGVIGRESEVLEIVNV 180

Query: 181 LIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKA 240
            +DLS  E LSVL IVGMGGLGKTALAKVIFNHE+++  FD+ +WVCVSEPF+IKKIL+A
Sbjct: 181 SVDLSYRENLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRAVWVCVSEPFLIKKILRA 240

Query: 241 ILETLNANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKS 300
           ILETLN++  GLDSKEALLQELQKL+NDKKYFLVLDDVW EN  LWNE K CLLKI+++S
Sbjct: 241 ILETLNSHFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 301 GSVILVTTRSDGVAETMETHPRYHL 326
           G+V++VTTRSD VAE METH RYHL
Sbjct: 301 GNVVVVTTRSDRVAEIMETHSRYHL 325

BLAST of Lcy10g009260 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 134.4 bits (337), Expect = 1.7e-31
Identity = 96/320 (30.00%), Postives = 173/320 (54.06%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAE + +F V+    K  +L + +     G +E++  L+  L  ++++L D D  K++  
Sbjct: 1   MAEAVVSFGVE----KLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
            ++ ++E ++DIV++ADD+++     +LR K E G    VR    +    LV R K A+ 
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELRGK-EKGIKKQVRTLACF----LVDRRKFASD 120

Query: 121 IMVVAKMLHEHYCATSAMGLVAVT-----AREFEPDFCQIQETDSFLEETGVIGREIEIL 180
           I  + K + E      ++G+  +      +   +    +I++T S   E+ ++G +  + 
Sbjct: 121 IEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSVE 180

Query: 181 EIVDKLIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIK 240
           E+VD L++    +++ V+S+ GMGG+GKT LA+ +F+H++VR+ FD   WVCVS+ F  K
Sbjct: 181 ELVDHLVE---NDSVQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTRK 240

Query: 241 KILKAILETLNANLSGLDSKE--ALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCL 300
            + + IL+ L     G+   +   L  EL +L+   +Y LVLDDVW E    W+  K   
Sbjct: 241 DVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAV- 300

Query: 301 LKITKKSGSVILVTTRSDGV 314
                K G  +L+T+R++G+
Sbjct: 301 --FPHKRGWKMLLTSRNEGL 303

BLAST of Lcy10g009260 vs. TAIR 10
Match: AT5G48620.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 125.9 bits (315), Expect = 5.9e-29
Identity = 91/310 (29.35%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 14  LKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKLQDIV 73
           L+K   L + +     G +E+L  L+  L+S++++L D D  K     ++ ++E ++D+V
Sbjct: 10  LEKLWDLLSRESERLQGIDEQLDGLKRQLRSLQSLLKDADAKKHGSDRVRNFLEDVKDLV 69

Query: 74  FEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANKIMVVAKMLHEHYC 133
           F+A+D+++      LR     G+   V+  V      L  R K+A+ I  + K + +   
Sbjct: 70  FDAEDIIESYVLNKLR-----GEGKGVKKHVRRLARFLTDRHKVASDIEGITKRISDVIG 129

Query: 134 ATSAMGLV-------AVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDKLIDLSN 193
              + G+        +++ +E +    +I++T     E+ ++G E  + E+V  L++   
Sbjct: 130 EMQSFGIQQIIDGVRSLSLQERQRVQREIRQTYPDSSESDLVGVEQSVEELVGHLVE--- 189

Query: 194 EEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKAILETL- 253
            +   V+SI GMGG+GKT LA+ +F+H++VR+ FD   WVCVS+ F +K + + IL+ L 
Sbjct: 190 NDIYQVVSIAGMGGIGKTTLARQVFHHDLVRRHFDGFAWVCVSQQFTLKHVWQRILQELQ 249

Query: 254 --NANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKSGSV 313
             + N+  +D + AL  +L +L+   +Y LVLDDVW +    W+  K       +K G  
Sbjct: 250 PHDGNILQMD-ESALQPKLFQLLETGRYLLVLDDVWKKED--WDRIKAV---FPRKRGWK 305

BLAST of Lcy10g009260 vs. TAIR 10
Match: AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 120.2 bits (300), Expect = 3.2e-27
Identity = 96/320 (30.00%), Postives = 172/320 (53.75%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKTVKLAAEQIGLAWGFNEELIKLRDSLQSVEAILCDNDRIKSQYP 60
           MAE + +F VQ+ L   +   +E++    G +E++  L+  L+ ++++L D D  K    
Sbjct: 1   MAEGVVSFGVQK-LWALLNRESERLN---GIDEQVDGLKRQLRGLQSLLKDADAKKHGSD 60

Query: 61  SMKPWVEKLQDIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANK 120
            ++ ++E ++D+VF+A+D+++      LR     G+   V+N V      L  R K+A+ 
Sbjct: 61  RVRNFLEDVKDLVFDAEDIIESYVLNKLR-----GEGKGVKNHVRRLACFLTDRHKVASD 120

Query: 121 IMVVAKMLHEHYCATSAMGL---VAVTAREFEPDFCQ--IQETDSFLEETGVIGREIEIL 180
           I  + K + +      ++G+   +    R       Q  I++T     E+ ++G E  + 
Sbjct: 121 IEGITKRISKVIGEMQSLGIQQQIIDGGRSLSLQDIQREIRQTFPNSSESDLVGVEQSVE 180

Query: 181 EIVDKLIDLSNEEALSVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIK 240
           E+V  ++++ N   + V+SI GMGG+GKT LA+ IF+H++VR+ FD   WVCVS+ F  K
Sbjct: 181 ELVGPMVEIDN---IQVVSISGMGGIGKTTLARQIFHHDLVRRHFDGFAWVCVSQQFTQK 240

Query: 241 KILKAILETLNANLSG-LDSKEALLQ-ELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCL 300
            + + IL+ L  +    L   E  +Q +L +L+   +Y +VLDDVW E    W+  K   
Sbjct: 241 HVWQRILQELRPHDGEILQMDEYTIQGKLFQLLETGRYLVVLDDVWKEED--WDRIKEV- 300

Query: 301 LKITKKSGSVILVTTRSDGV 314
               +K G  +L+T+R++GV
Sbjct: 301 --FPRKRGWKMLLTSRNEGV 303

BLAST of Lcy10g009260 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.6e-26
Identity = 90/301 (29.90%), Postives = 159/301 (52.82%), Query Frame = 0

Query: 32  NEELI-KLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKLQDIVFEADDLLDELAYEDLRL 91
           NE L+ +L  +L ++ A+L D +  +   P ++ WV +L+D+V+ A+D LD++A E LRL
Sbjct: 35  NENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRL 94

Query: 92  KV--ETGKNVMVR---------NFVSYSKNPLVFRLKMANKIMVVAKMLHEHYCATSAMG 151
            +  E+  +  +R         +F+  +   L  RL+   K+ +  + L           
Sbjct: 95  NIGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLE---KVTIRLERLASQRNILGLKE 154

Query: 152 LVAVTAREFEPDFCQIQETDSFLEETGVIGREIEILEIVDKLI-DLSNEEALSVLSIVGM 211
           L A+  ++  P       T S ++E+ V GR+ +  EI+  LI +   +  ++V++IVG+
Sbjct: 155 LTAMIPKQRLP-------TTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGI 214

Query: 212 GGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKAILETLNANLSGLDSKEAL 271
           GG+GKT L+++++N + VR +F   +W  VSE F + KI K + E++ +        + L
Sbjct: 215 GGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVL 274

Query: 272 LQELQKLMNDK--KYFLVLDDVWTENSNLWNEFKTCLLKITKKSGSVILVTTRSDGVAET 318
             +L++ +      + LVLDD+W EN   W+  +   +   +  GS ILVTTRS  VA  
Sbjct: 275 QVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQ--GSQILVTTRSQRVASI 323

BLAST of Lcy10g009260 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 117.5 bits (293), Expect = 2.1e-26
Identity = 85/321 (26.48%), Postives = 162/321 (50.47%), Query Frame = 0

Query: 13  VLKKTVKLAAEQIGLAWGFNEE--LIKLRDSLQSVEAILCDNDRIKSQYPSMKPWVEKLQ 72
           V+ + +  + E + L  G +    L +L+ +L +   +L D D+       +K W+  ++
Sbjct: 12  VMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHVREVKHWLTGIK 71

Query: 73  DIVFEADDLLDELAYEDLRLKVETGKNVMVRNFVSYSKNPLVFRLKMANKIMVVAKMLHE 132
           D  F+A+D+LDEL  E LR +V      +   F +        + K+  K+  V ++L  
Sbjct: 72  DAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEH 131

Query: 133 HYCATSAMGLVAVTAREFEPDFCQIQET-DSFLEETGVIGREIEILEIVDKLIDLSNEEA 192
           H      +GL   +    EP + Q   +    L +  ++GR  + L +V+ L  LS++E 
Sbjct: 132 HVKHIEVIGLKEYSETR-EPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLL--LSDDEI 191

Query: 193 L----SVLSIVGMGGLGKTALAKVIFNHEVVRKFFDKTIWVCVSEPFVIKKILKAILETL 252
                +V+S+VGM G+GKT L +++FN   V + F+  +W+     F +  + KA+L+ +
Sbjct: 192 SIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDI 251

Query: 253 NANLSGLDSKEALLQELQKLMNDKKYFLVLDDVWTENSNLWNEFKTCLLKITKKSGSVIL 312
            ++    +   +L  +L+K ++ K++ LVLDD W+E+ + W  F+        + GS I+
Sbjct: 252 TSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWESFQVAF--TDAEEGSKIV 311

Query: 313 VTTRSDGVAETMETHPRYHLR 327
           +TTRS+ V+   +    Y ++
Sbjct: 312 LTTRSEIVSTVAKAEKIYQMK 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XA401.5e-3735.26Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA425.4e-3533.84Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ95.9e-3432.72Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA392.9e-3331.40Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
P0C8S12.3e-3030.00Probable disease resistance RPP8-like protein 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CUL23.9e-12972.56putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5D3CZ176.6e-12973.85Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7TSS66.6e-12973.85Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3BXI26.6e-12973.85putative disease resistance protein RGA3 OS=Cucumis melo OX=3656 GN=LOC103494528... [more]
A0A0A0KW561.6e-12773.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015840 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022145224.18.0e-12972.56putative disease resistance protein RGA3 [Momordica charantia][more]
XP_008453966.11.4e-12873.85PREDICTED: putative disease resistance protein RGA3 [Cucumis melo] >TYK16977.1 p... [more]
KAA0044611.11.4e-12873.85putative disease resistance protein RGA3 [Cucumis melo var. makuwa][more]
XP_038874765.12.6e-12771.00putative disease resistance protein RGA3 [Benincasa hispida][more]
XP_004152292.13.4e-12773.23putative disease resistance protein RGA3 [Cucumis sativus] >KGN53094.1 hypotheti... [more]
Match NameE-valueIdentityDescription
AT1G53350.11.7e-3130.00Disease resistance protein (CC-NBS-LRR class) family [more]
AT5G48620.15.9e-2929.35Disease resistance protein (CC-NBS-LRR class) family [more]
AT5G35450.13.2e-2730.00Disease resistance protein (CC-NBS-LRR class) family [more]
AT3G14470.11.6e-2629.90NB-ARC domain-containing disease resistance protein [more]
AT3G14460.12.1e-2626.48LRR and NB-ARC domains-containing disease resistance protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 265..279
score: 43.67
coord: 192..207
score: 49.53
NoneNo IPR availableGENE3D1.20.5.4130coord: 8..122
e-value: 5.4E-15
score: 57.4
NoneNo IPR availablePANTHERPTHR23155:SF860OS10G0130600 PROTEINcoord: 37..328
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 9..95
e-value: 1.3E-17
score: 63.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 154..322
e-value: 7.5E-39
score: 135.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 158..326
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 176..322
e-value: 3.3E-34
score: 118.2
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 37..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g009260.1Lcy10g009260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding