Lcy10g008940 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g008940
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr10: 35319781 .. 35321043 (-)
RNA-Seq ExpressionLcy10g008940
SyntenyLcy10g008940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCCGAAGACATTTTGGCTATTCCATTGAGTAATAGGAATAACAAGGATGAAATCTATTGAAGCGGAGAGGCAAAAGGGGTGTTTAGTGTTAAAAGTGCATACCACATAGCTACAAACGTTCAGAAAGCTAGCGAGGCCTCTGAATCCGGGATAAATTCTCAAAAGAACAGATGGAGGTCCATTTGGGATCTTAACATCATTCCAAGAGCAAAAATTGGCCTCTGGAAAATTATAAAAAACCTTATCCCCTCTAAACAAAATCTATTACAAAGAGGAATGGACATTAACCAAGTTTGTGACTTGTGCAGATGTGCAAAAGAAAGCCCAGACCACTTATTTTGGAGGTGCAAGAAGGTAAGGAAAATATGGGAAAGGTTTTTCCCCAATCTTACAAAAACTATACTGTCTTGCAGGCCAGGGATGGATATGGGAGATGCGTGGGAGATTATATTCAAGCATCACAGTAAAGAAGAATGGGAGAGAATAGGCATCATCATTTGGACGATTTGGAATTTTAGGAACCAAGTAGCAGTAAACAAGCAAAATCCAGAATAAGAGCAGTTATACTAGTGGATTCAAAAGAATTTGGAAGATCAACGAAAATGTACCTCGCCAAATCTTTCGGAGATTCTTCTGGAGAGCCTTTGGAATCACGATAGGTGGACCCCTCCCCCCCCCCCCCCCCCCCGAAATTTGCTGAAAATAAATTCGGATGCCTCCTAGAATGAAGCCACTGGCATTGGAGGAGTCGGTTGGATCACCCGCGATTCTTCAGGATCTCTGGTTCAAGCGGGATACAAAAGGCTAAATCGAAAATGGAACATTAAACTTCTCGAATTGGCTGTGATTAAGGAAGGTTTGAACTCTTACCTCGCGAGCGAGCACTCTAGAAATTCTGCTCTAATTTTGGAAGCTGATGCCCTAGAAGTTATCAAAGCCCTGACTTGCGAAGGTTACGACATTTCTGAAGCCAAGGCGTTTTTGATTGATATTGAACATCTGGCGGCAAGAGTGGGAGTTGTTTCCTTTGTGAAATGCCCAAGAGCGGGCAATCAAGTGACACATTCCCTTGCGCGTGCAGCGGCGGGCTTCCCTCCGGTGTCTTCTCCGTCAATTGTTGATGCCGATGGAATTTTTGAGCTTTCTTTCCCTTCCATGCTGGAAGAAGCTTTATTTTGTAGGGATGATGATATCCCCGGTTGGTTAACCTCTTTAATTGAAGAGGATATTGTTGCAACTAACTCTTATCAGTTTTAA

mRNA sequence

ATGGACGCCGAAGACATTTTGGCTATTCCATTGAATGTGCAAAAGAAAGCCCAGACCACTTATTTTGGAGGTGCAAGAAGGCCAGGGATGGATATGGGAGATGCGTGGGAGATTATATTCAAGCATCACAGTAAAGAAGAATGGGAGAGAATAGGCATCATCATTTGGACGATTTGGAATTTTAGGAACCAAGTAGCAAATGAAGCCACTGGCATTGGAGGAGTCGGTTGGATCACCCGCGATTCTTCAGGATCTCTGGTTCAAGCGGGATACAAAAGGCTAAATCGAAAATGGAACATTAAACTTCTCGAATTGGCTGTGATTAAGGAAGGTTTGAACTCTTACCTCGCGAGCGAGCACTCTAGAAATTCTGCTCTAATTTTGGAAGCTGATGCCCTAGAAGTTATCAAAGCCCTGACTTGCGAAGGTTACGACATTTCTGAAGCCAAGGCGTTTTTGATTGATATTGAACATCTGGCGGCAAGAGTGGGAGTTGTTTCCTTTGTGAAATGCCCAAGAGCGGGCAATCAAGTGACACATTCCCTTGCGCGTGCAGCGGCGGGCTTCCCTCCGGTGTCTTCTCCGTCAATTGTTGATGCCGATGGAATTTTTGAGCTTTCTTTCCCTTCCATGCTGGAAGAAGCTTTATTTTGTAGGGATGATGATATCCCCGGTTGGTTAACCTCTTTAATTGAAGAGGATATTGTTGCAACTAACTCTTATCAGTTTTAA

Coding sequence (CDS)

ATGGACGCCGAAGACATTTTGGCTATTCCATTGAATGTGCAAAAGAAAGCCCAGACCACTTATTTTGGAGGTGCAAGAAGGCCAGGGATGGATATGGGAGATGCGTGGGAGATTATATTCAAGCATCACAGTAAAGAAGAATGGGAGAGAATAGGCATCATCATTTGGACGATTTGGAATTTTAGGAACCAAGTAGCAAATGAAGCCACTGGCATTGGAGGAGTCGGTTGGATCACCCGCGATTCTTCAGGATCTCTGGTTCAAGCGGGATACAAAAGGCTAAATCGAAAATGGAACATTAAACTTCTCGAATTGGCTGTGATTAAGGAAGGTTTGAACTCTTACCTCGCGAGCGAGCACTCTAGAAATTCTGCTCTAATTTTGGAAGCTGATGCCCTAGAAGTTATCAAAGCCCTGACTTGCGAAGGTTACGACATTTCTGAAGCCAAGGCGTTTTTGATTGATATTGAACATCTGGCGGCAAGAGTGGGAGTTGTTTCCTTTGTGAAATGCCCAAGAGCGGGCAATCAAGTGACACATTCCCTTGCGCGTGCAGCGGCGGGCTTCCCTCCGGTGTCTTCTCCGTCAATTGTTGATGCCGATGGAATTTTTGAGCTTTCTTTCCCTTCCATGCTGGAAGAAGCTTTATTTTGTAGGGATGATGATATCCCCGGTTGGTTAACCTCTTTAATTGAAGAGGATATTGTTGCAACTAACTCTTATCAGTTTTAA

Protein sequence

MDAEDILAIPLNVQKKAQTTYFGGARRPGMDMGDAWEIIFKHHSKEEWERIGIIIWTIWNFRNQVANEATGIGGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLASEHSRNSALILEADALEVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKCPRAGNQVTHSLARAAAGFPPVSSPSIVDADGIFELSFPSMLEEALFCRDDDIPGWLTSLIEEDIVATNSYQF
Homology
BLAST of Lcy10g008940 vs. ExPASy TrEMBL
Match: A0A7C9CLE4 (RNase H domain-containing protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.2e-07
Identity = 41/127 (32.28%), Postives = 63/127 (49.61%), Query Frame = 0

Query: 63  NQVANEATG-IGGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLASEHS 122
           N  A+ A+G   G+G + RD  G LV +   ++   WN ++ E   ++ G+     ++  
Sbjct: 4   NTDASLASGEFRGLGAVLRDHLGGLVASACAKMEGNWNAEISEACAVRFGMK---VAKSL 63

Query: 123 RNSALILEADALEVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKCPRAGNQVTHS 182
             + +ILE DA EV+ AL+C+ Y +  A  F+ D   L      V FV      N+V H 
Sbjct: 64  GFNQVILETDAAEVVHALSCKTYKLDYASTFVKDCHSLIKDFSSVKFVHAKHCCNEVAHE 123

Query: 183 LARAAAG 189
           LAR A G
Sbjct: 124 LARVALG 127

BLAST of Lcy10g008940 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.6e-07
Identity = 45/129 (34.88%), Postives = 65/129 (50.39%), Query Frame = 0

Query: 59  WNFRNQVANEA-TGIGGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLA 118
           W      A  A T  GG+GWI RD  G +++A  + +  + NI  LE+  I EGL + + 
Sbjct: 87  WKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRA-IR 146

Query: 119 SEHSRNSALILEADALEVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKCPRAGNQ 178
            EH R   + LE+D+LE I  L  +  D +E    L +I  +   + +VS     R  N+
Sbjct: 147 QEHCR--PIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANK 206

Query: 179 VTHSLARAA 187
           V H LAR A
Sbjct: 207 VAHGLARRA 212

BLAST of Lcy10g008940 vs. ExPASy TrEMBL
Match: A0A803NZB5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.6e-07
Identity = 49/155 (31.61%), Postives = 74/155 (47.74%), Query Frame = 0

Query: 44  SKEEWERIGIIIWTIWNFRNQV----------ANEATGIGGVGWITRDSSGSLVQAGYKR 103
           SK E E++   +WTIWN RN+V           + + G+ GVG I RDS+G +V A  K+
Sbjct: 150 SKAEMEQLFCTLWTIWNERNRVVHGQKARPASVDSSRGVIGVGAILRDSNGVVVAALSKK 209

Query: 104 LNRKWNIKLLELAVIKEGLNSYLASEHSRNSALILEADALEVIKALTCEGYDISEAKAFL 163
           L   +    +E   +   LN  +  +   +   I+E+DAL V+ AL       S     +
Sbjct: 210 LIGNFRSHEMEATALFHSLNWAIQQQLPLS---IVESDALMVVNALRAPFNSNSSFSDLI 269

Query: 164 IDIEHLAARVGVVSFVKCPRAGNQVTHSLARAAAG 189
           IDI  L + +  V+     R+ N   H LA+ A G
Sbjct: 270 IDISCLLSFLSSVTISHVKRSANIAAHDLAKFALG 301

BLAST of Lcy10g008940 vs. ExPASy TrEMBL
Match: A0A803Q3C1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.1e-07
Identity = 68/261 (26.05%), Postives = 101/261 (38.70%), Query Frame = 0

Query: 40   FKHHSKEEWERIGIIIWTIWNFRNQVANEATGIG-------------------------- 99
            F+   KE+ E +  + W IWN RN     ++G+                           
Sbjct: 994  FQRMEKEDRELLVAVAWAIWNARNDRNAHSSGLELLSAGLNQGDRAEHWVPPIENNVKVN 1053

Query: 100  ------------GVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLASEHS 159
                        GVGW+ RD+ G L+    K  N +   +L E   I+E L S++ S  S
Sbjct: 1054 VDATIFEASREFGVGWVARDTRGLLINGHTKLFNVQATPELAEAVEIREAL-SWIKS--S 1113

Query: 160  RNSALILEADALEVIKALTCE-------GYDISEAKAFLIDIEHLAARVGVVSFVKCPRA 219
                ++LE D L V++AL          G  ++E KA L D+     R   + F++  R+
Sbjct: 1114 GLQQVVLETDCLSVVQALRSSIVMISTFGQVVNECKALLNDL-----RTVFIYFIR--RS 1173

Query: 220  GNQVTHSLARAAAGFP--------------PVSSPSIVDADGIFELSFPSMLEEALFCRD 242
             N V H LARA+  FP              PV    +VDA    E +F           +
Sbjct: 1174 ANTVAHELARASVSFPDCNFDLDSVLTVLLPVLVTDMVDA--FTESAFKGNPAAVCLVDE 1233

BLAST of Lcy10g008940 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.4e-06
Identity = 45/135 (33.33%), Postives = 65/135 (48.15%), Query Frame = 0

Query: 59  WNFRNQVANEA-TGIGGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLA 118
           W      A  A T  GG+GWI RD  G +++A  + +  + NI  LE+  I EGL + + 
Sbjct: 87  WKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRA-IR 146

Query: 119 SEHSRN------SALILEADALEVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKC 178
            EH R         + LE+D+LE I  L  +  D +E    L +I  +   + +VS    
Sbjct: 147 QEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHI 206

Query: 179 PRAGNQVTHSLARAA 187
            R  N+V H LAR A
Sbjct: 207 SREANKVAHDLARRA 220

BLAST of Lcy10g008940 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 66.6 bits (161), Expect = 3.3e-07
Identity = 45/129 (34.88%), Postives = 65/129 (50.39%), Query Frame = 0

Query: 59  WNFRNQVANEA-TGIGGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLA 118
           W      A  A T  GG+GWI RD  G +++A  + +  + NI  LE+  I EGL + + 
Sbjct: 87  WKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRA-IR 146

Query: 119 SEHSRNSALILEADALEVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKCPRAGNQ 178
            EH R   + LE+D+LE I  L  +  D +E    L +I  +   + +VS     R  N+
Sbjct: 147 QEHCR--PIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANK 206

Query: 179 VTHSLARAA 187
           V H LAR A
Sbjct: 207 VAHGLARRA 212

BLAST of Lcy10g008940 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 63.5 bits (153), Expect = 2.8e-06
Identity = 45/135 (33.33%), Postives = 65/135 (48.15%), Query Frame = 0

Query: 59  WNFRNQVANEA-TGIGGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLA 118
           W      A  A T  GG+GWI RD  G +++A  + +  + NI  LE+  I EGL + + 
Sbjct: 87  WKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRA-IR 146

Query: 119 SEHSRN------SALILEADALEVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKC 178
            EH R         + LE+D+LE I  L  +  D +E    L +I  +   + +VS    
Sbjct: 147 QEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHI 206

Query: 179 PRAGNQVTHSLARAA 187
            R  N+V H LAR A
Sbjct: 207 SREANKVAHDLARRA 220

BLAST of Lcy10g008940 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 62.4 bits (150), Expect = 6.3e-06
Identity = 45/145 (31.03%), Postives = 75/145 (51.72%), Query Frame = 0

Query: 43  HSKEEWERIGIIIWTIWNFRNQVANEATGIGGVGWITRDSSGSLVQAGYKRLNRKWNIKL 102
           ++K +WE   + IWT+    +   +++T  GG+GWI R   G +V AG + +    N+KL
Sbjct: 66  NNKLKWEPPPMHIWTL--NADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKL 125

Query: 103 LELAVIKEGLNSYLASEHSRNSALILEADALEVIKALTCEGYDISEAKAFLIDIEHLAAR 162
           LE + I EGL +       R   L +E D+ EV   L  +  D+++    + +I +L   
Sbjct: 126 LEASAILEGLRNLTNLGVLR--PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDS 185

Query: 163 VGVVSFVKCPRAGNQVTHSLARAAA 188
             +++F K  R  N   HSLA+ A+
Sbjct: 186 CEILAFAKVGRETNGCAHSLAQRAS 206

BLAST of Lcy10g008940 vs. NCBI nr
Match: XP_021808158.1 (uncharacterized protein LOC110751913 [Prunus avium])

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-05
Identity = 38/111 (34.23%), Postives = 61/111 (54.95%), Query Frame = 0

Query: 73  GGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLASEHSRNSALILEADA 132
           GGVGW+ RDS G L+ AG +   R  +  L+EL  I+  L+   A  H + + +++E+D 
Sbjct: 99  GGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHALS---ACAHFQLTHVMVESDT 158

Query: 133 LEVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKCPRAGNQVTHSLA 184
              I  L  +    S+ +  + DI+ L A    VSFV  P++ N+V H++A
Sbjct: 159 QRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAPKSCNRVAHTVA 206

BLAST of Lcy10g008940 vs. NCBI nr
Match: XP_030509250.1 (uncharacterized protein LOC115723928 [Cannabis sativa])

HSP 1 Score: 60.1 bits (144), Expect = 3.1e-05
Identity = 48/154 (31.17%), Postives = 75/154 (48.70%), Query Frame = 0

Query: 68  EATGIGGVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLASEHSRNSALI 127
           EA+   GVGW+ RD+ G L+    K  N +   +L E   I+E L S++ S  S    ++
Sbjct: 138 EASREFGVGWVARDTRGLLINGHTKLFNVQATPELAEAVEIREAL-SWIKS--SGLQQVV 197

Query: 128 LEADALEVIKALTCE-------GYDISEAKAFLIDIEHLAARVGVVSFVKCPRAGNQVTH 187
           LE D L V++AL          G  ++E KA L D+     R   + F++  R+ N V H
Sbjct: 198 LETDCLSVVQALRSSIVMISTFGQVVNECKALLNDL-----RTVFIYFIR--RSANTVAH 257

Query: 188 SLARAAAGFPPVSSPSIVDADGIFELSFPSMLEE 215
            LARA+  FP  +     D D +  +  P ++ +
Sbjct: 258 ELARASVSFPDCN----FDLDSVLTVLLPVLVTD 277

BLAST of Lcy10g008940 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 5.1e-06
Identity = 34/116 (29.31%), Postives = 56/116 (48.28%), Query Frame = 0

Query: 74  GVGWITRDSSGSLVQAGYKRLNRKWNIKLLELAVIKEGLNSYLASEHSRNSALILEADAL 133
           G+GWI R+ SG ++  G + L R  N+   EL  ++  + +     + R   +I E+DA 
Sbjct: 161 GIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSRFNYKR---IIFESDAQ 220

Query: 134 EVIKALTCEGYDISEAKAFLIDIEHLAARVGVVSFVKCPRAGNQVTHSLARAAAGF 190
            ++  L  + +  +   A L DI+ L      V F   PR GN+V   +AR +  F
Sbjct: 221 ALVNLLNSDDFWPTLQPA-LEDIQQLLHHFEEVKFEFTPRGGNKVADRIARESISF 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A7C9CLE41.2e-0732.28RNase H domain-containing protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1[more]
A0A6J1CP261.6e-0734.88uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A803NZB51.6e-0731.61Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803Q3C12.1e-0726.05Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DSV11.4e-0633.33uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
Match NameE-valueIdentityDescription
XP_022143535.13.3e-0734.88uncharacterized protein LOC111013412 [Momordica charantia][more]
XP_022156777.12.8e-0633.33uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_022155262.16.3e-0631.03uncharacterized protein LOC111022403 [Momordica charantia][more]
XP_021808158.11.8e-0534.23uncharacterized protein LOC110751913 [Prunus avium][more]
XP_030509250.13.1e-0531.17uncharacterized protein LOC115723928 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
AT2G34320.15.1e-0629.31Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 62..188
e-value: 9.4E-7
score: 30.9
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 67..186
e-value: 4.6E-14
score: 52.3
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 71..188
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 71..188
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 71..184
e-value: 5.78228E-14
score: 64.6428
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 69..192

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g008940.1Lcy10g008940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity