Lcy10g008000 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g008000
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPoly(A)-specific ribonuclease
LocationChr10: 33189959 .. 33190825 (-)
RNA-Seq ExpressionLcy10g008000
SyntenyLcy10g008000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTTGGTTCGACAAGTTTGGTCTCACAATCTCCCACAAGAGCTGGCCCTTCTCAACGCCTGTCTTTCACAGTTTCCTGTGGTCGCCTTCGACACCGAGTTCCCCGGGTTTCTCCGATGCACTCCGAGAGGTGCTCCACAGGAACACTTCTATGAGGATCTCAAATTCAATGTCAACCATTTGAAGATTCTCCAACTGGGTCTCACTCTTGCCAACGAGAGTGGCGATGTTGCCATGACATGGGAGTTCAACTTCAGTGATTTCGATGAAACCACAGACCTCCACAGTCCAACATCCATCCAATTCTTGAAAAGTAAAGGCTTCGATTTCAACAAACATAGGAACCAAGGAATCCCATCTCCTCAATTTCGACGCGCATTTCTTCCCATCTGTTGCAGCAATCGCATTGCCAAATGGCTCACCTTCCACGGCTTCTACGACGTCGCTTACCTTCTCAAGCTACTCATGATCAAAACCATGCCGCAATCCCTCGCCGTCTTCGCCGCCAAAGCCCAGCGTCTCCTCGGCACCGTCAGCGACCTCAAGCACATCATTCGCAACTGCGATTACTTACTCAACGGCGAGCTCGGACTTGAAAAACTCGCCAAGTTACTCGATGTCGAGCGGAACGGCACTGCCCACCATGCTGGCTCCGACAGTCTCTTGACCGCTTCTGTCTACGTGAAGATGAAGAAGAGGTTCGAGTTTTCGCCGGAGCATTGCGACGGGTTCTTATACGGTCTGCATTACAGAATCCGACGACGTCCAGTAATTGGGAACGTCGTCATTCCCCCATTTCGTCAACCGCCTGTTAGTGTCGTTAGGCATAGTTTGTTTTCCGGTCATGTTATGGTTTCCGGTTAA

mRNA sequence

ATGACTTTGGTTCGACAAGTTTGGTCTCACAATCTCCCACAAGAGCTGGCCCTTCTCAACGCCTGTCTTTCACAGTTTCCTGTGGTCGCCTTCGACACCGAGTTCCCCGGGTTTCTCCGATGCACTCCGAGAGGTGCTCCACAGGAACACTTCTATGAGGATCTCAAATTCAATGTCAACCATTTGAAGATTCTCCAACTGGGTCTCACTCTTGCCAACGAGAGTGGCGATGTTGCCATGACATGGGAGTTCAACTTCAGTGATTTCGATGAAACCACAGACCTCCACAGTCCAACATCCATCCAATTCTTGAAAAGTAAAGGCTTCGATTTCAACAAACATAGGAACCAAGGAATCCCATCTCCTCAATTTCGACGCGCATTTCTTCCCATCTGTTGCAGCAATCGCATTGCCAAATGGCTCACCTTCCACGGCTTCTACGACGTCGCTTACCTTCTCAAGCTACTCATGATCAAAACCATGCCGCAATCCCTCGCCGTCTTCGCCGCCAAAGCCCAGCGTCTCCTCGGCACCGTCAGCGACCTCAAGCACATCATTCGCAACTGCGATTACTTACTCAACGGCGAGCTCGGACTTGAAAAACTCGCCAAGTTACTCGATGTCGAGCGGAACGGCACTGCCCACCATGCTGGCTCCGACAGTCTCTTGACCGCTTCTGTCTACGTGAAGATGAAGAAGAGGTTCGAGTTTTCGCCGGAGCATTGCGACGGGTTCTTATACGGTCTGCATTACAGAATCCGACGACGTCCAGTAATTGGGAACGTCGTCATTCCCCCATTTCGTCAACCGCCTGTTAGTGTCGTTAGGCATAGTTTGTTTTCCGGTCATGTTATGGTTTCCGGTTAA

Coding sequence (CDS)

ATGACTTTGGTTCGACAAGTTTGGTCTCACAATCTCCCACAAGAGCTGGCCCTTCTCAACGCCTGTCTTTCACAGTTTCCTGTGGTCGCCTTCGACACCGAGTTCCCCGGGTTTCTCCGATGCACTCCGAGAGGTGCTCCACAGGAACACTTCTATGAGGATCTCAAATTCAATGTCAACCATTTGAAGATTCTCCAACTGGGTCTCACTCTTGCCAACGAGAGTGGCGATGTTGCCATGACATGGGAGTTCAACTTCAGTGATTTCGATGAAACCACAGACCTCCACAGTCCAACATCCATCCAATTCTTGAAAAGTAAAGGCTTCGATTTCAACAAACATAGGAACCAAGGAATCCCATCTCCTCAATTTCGACGCGCATTTCTTCCCATCTGTTGCAGCAATCGCATTGCCAAATGGCTCACCTTCCACGGCTTCTACGACGTCGCTTACCTTCTCAAGCTACTCATGATCAAAACCATGCCGCAATCCCTCGCCGTCTTCGCCGCCAAAGCCCAGCGTCTCCTCGGCACCGTCAGCGACCTCAAGCACATCATTCGCAACTGCGATTACTTACTCAACGGCGAGCTCGGACTTGAAAAACTCGCCAAGTTACTCGATGTCGAGCGGAACGGCACTGCCCACCATGCTGGCTCCGACAGTCTCTTGACCGCTTCTGTCTACGTGAAGATGAAGAAGAGGTTCGAGTTTTCGCCGGAGCATTGCGACGGGTTCTTATACGGTCTGCATTACAGAATCCGACGACGTCCAGTAATTGGGAACGTCGTCATTCCCCCATTTCGTCAACCGCCTGTTAGTGTCGTTAGGCATAGTTTGTTTTCCGGTCATGTTATGGTTTCCGGTTAA

Protein sequence

MTLVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVNHLKILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNKHRNQGIPSPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSPEHCDGFLYGLHYRIRRRPVIGNVVIPPFRQPPVSVVRHSLFSGHVMVSG
Homology
BLAST of Lcy10g008000 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 6.9e-47
Identity = 107/259 (41.31%), Postives = 148/259 (57.14%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRG---APQEHFYEDLKFNVN 63
           +R+VW+ NL  E+AL+   +  FP VA DTEFPG + C P G      E+ YE LK NVN
Sbjct: 12  IREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIV-CRPVGTFKTNTEYHYETLKTNVN 71

Query: 64  HLKILQLGLTLANESGDVA--------MTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFN 123
            LK++QLGLT ++E G++           W+FNF +FD  +D+++  SI+ L+  G DF 
Sbjct: 72  ILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIELLRQSGIDFV 131

Query: 124 KHRNQGIPSPQFRRAFLPI-CCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAK 183
           K+   GI S +F    +      N    W+TFH  YD  YLLKLL  + +P++   F   
Sbjct: 132 KNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGFFEM 191

Query: 184 AQRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKM 243
                  V D+KH+++ C+ L     GL KLA+LLDVER G  H AGSDSLLT+  + K+
Sbjct: 192 ISVYFPRVYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTFRKL 251

Query: 244 KKRFEF-SPEHCDGFLYGL 250
           ++ F   S E   G LYGL
Sbjct: 252 QENFFIGSMEKYSGVLYGL 266

BLAST of Lcy10g008000 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.5e-46
Identity = 108/258 (41.86%), Postives = 145/258 (56.20%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRG---APQEHFYEDLKFNVN 63
           +R+VW+ NL +E+ L+   +  FP VA DTEFPG +   P G   +  ++ YE LK NVN
Sbjct: 12  IREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVN 71

Query: 64  HLKILQLGLTLANESG-------DVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNK 123
            LK++QLGLT +NE G       D    W+FNF +FD  +D+ +  SI+ LK  G D  K
Sbjct: 72  ILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIELLKQSGIDLAK 131

Query: 124 HRNQGIPSPQFRRAFLPI-CCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKA 183
           +   GI S +F    +      N    W+TFH  YD  YLLKLL  + +P S   F    
Sbjct: 132 NTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLI 191

Query: 184 QRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMK 243
                TV D+KH+++ C+ L     GL KLA+LL+VER G  H AGSDSLLT+  + K+K
Sbjct: 192 NVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLK 251

Query: 244 KRFEFSPEH-CDGFLYGL 250
           + F   P H   G LYGL
Sbjct: 252 ENFFVGPLHKYSGVLYGL 265

BLAST of Lcy10g008000 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.2e-45
Identity = 97/251 (38.65%), Postives = 147/251 (58.57%), Query Frame = 0

Query: 3   LVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVNHL 62
           L R+VW  N+ +E+A +  CL +FP++AFDTE+PG +  T   +  +  Y  +K NV + 
Sbjct: 8   LARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENT 67

Query: 63  KILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNKHRNQGIPSP 122
           K++Q G TL N  G++   WE NFS+F + +D  +  SI+FL+  G D  K R++G+   
Sbjct: 68  KLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGV--D 127

Query: 123 QFRRAFLP----ICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGT 182
            F   F P    +  S +  +++TF G YD AY L +L    +P++   FA +  ++ G 
Sbjct: 128 MFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGKLPETHGEFATEVVKVFGQ 187

Query: 183 VSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFS 242
           V D K +   C+  L   LGL KLA+LL + R G AHHAGSDSL+TA V++K+K  +E S
Sbjct: 188 VYDTKVMAGFCEG-LGEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYEDS 247

Query: 243 PEHCDGFLYGL 250
                G +YG+
Sbjct: 248 -RFARGLIYGI 254

BLAST of Lcy10g008000 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.1e-44
Identity = 102/264 (38.64%), Postives = 154/264 (58.33%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQ--------EHFYEDL 63
           +R+VW+HNL QE+AL+   +  FP VA DTEFPG +  T    P         E+ Y+ L
Sbjct: 16  IREVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTL 75

Query: 64  KFNVNHLKILQLGLTLANESGDV-------AMTWEFNFSDFDETTDLHSPTSIQFLKSKG 123
           K NVN LK++QLGLTL++E G++          W+FNF +F+  +D+ +  SI+ L+   
Sbjct: 76  KANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIELLRKSA 135

Query: 124 FDFNKHRNQGIPSPQFRRAFL--PICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLA 183
            D  K+   G+ + +F    +   +  +++I  W+TFH  YD  YLLKLL  K +P+ ++
Sbjct: 136 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 195

Query: 184 VFAAKAQRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTAS 243
            F  + ++    V D+K+++  C  L     GLEK+A+LL V+R G +H AGSDSLLT  
Sbjct: 196 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 255

Query: 244 VYVKMKK-RFEFSPEHCDGFLYGL 250
            ++KMK+  F  S     GFL+GL
Sbjct: 256 TFIKMKEFFFTGSLLKYSGFLFGL 275

BLAST of Lcy10g008000 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 9.6e-41
Identity = 96/228 (42.11%), Postives = 131/228 (57.46%), Query Frame = 0

Query: 6   QVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVNHLKIL 65
           +VW  N   E+  +  CL     +A DTEFPG L+ TP  A  E  Y D+KFNV++  ++
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 66  QLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNKHRNQGIPSPQFR 125
           QLGLTL  +   +  TWE N SDF+E+  L +  SI FLK+ G D +K R +GI   +F 
Sbjct: 64  QLGLTLFGKG--ITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 126 RAFLPICCSNR-IAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGT-VSDLK 185
             F  I        +W+TF G YD AYLLK L  K +P++   F    Q+LLG  V D+K
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGLTRKPLPETSKEFDETVQQLLGRFVYDVK 183

Query: 186 HIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKM 232
            +   C   L+   GL+++A +L + R G AHHAGSDS LTA V+ K+
Sbjct: 184 KMAGLCSG-LSSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

BLAST of Lcy10g008000 vs. ExPASy TrEMBL
Match: A0A5A7TN62 (Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002920 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 2.4e-95
Identity = 175/255 (68.63%), Postives = 205/255 (80.39%), Query Frame = 0

Query: 1   MTLVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVN 60
           M+ VRQVW HNL QEL +LN  L +FPV+A DTEFPGFLR T RGAP+EHFY+DLKFN+N
Sbjct: 1   MSFVRQVWYHNLTQELVILNDQLFKFPVIAMDTEFPGFLRSTLRGAPEEHFYQDLKFNLN 60

Query: 61  HLKILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKS-KGFDFNKHRNQGI 120
           HLKILQLGLTL +E+  V ++W F FSDFDE TD  SPTSIQ+LK+ KGF+F K R  GI
Sbjct: 61  HLKILQLGLTLMDENEHVGLSWVFTFSDFDEQTDFSSPTSIQYLKNKKGFEFKKQRKDGI 120

Query: 121 PSPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTV 180
           PS +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL+MI+TMP+S+  FA  AQR LGTV
Sbjct: 121 PSAEFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMMIRTMPESMVEFAIIAQRHLGTV 180

Query: 181 SDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSP 240
           +DLKH+IRNC+YL+NGELGL++LA+LL+V  N T  + GSDSLL A  Y KMKK  +FS 
Sbjct: 181 NDLKHMIRNCEYLMNGELGLKRLAELLNV--NDTIFNGGSDSLLIALAYAKMKKTLKFSS 240

Query: 241 EHCDGFLYGLHYRIR 255
           E  DGFLYG HYRIR
Sbjct: 241 EITDGFLYGFHYRIR 253

BLAST of Lcy10g008000 vs. ExPASy TrEMBL
Match: A0A5D3D333 (Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001870 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 4.1e-95
Identity = 174/255 (68.24%), Postives = 206/255 (80.78%), Query Frame = 0

Query: 1   MTLVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVN 60
           M+ VR+VW HN  QELA+LN  L +FPV+A DTEFPGFLR TPRGAP+EHFY+DLKFN+N
Sbjct: 1   MSFVRKVWYHNFIQELAILNDQLFKFPVIAMDTEFPGFLRSTPRGAPEEHFYQDLKFNLN 60

Query: 61  HLKILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKS-KGFDFNKHRNQGI 120
           HLKILQLGLTL +E+  V ++W F FSDFDE TD  SPTSIQ+LK+ KGF+F K R  GI
Sbjct: 61  HLKILQLGLTLMDENEHVGLSWVFTFSDFDEQTDFSSPTSIQYLKNKKGFEFKKQRKDGI 120

Query: 121 PSPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTV 180
           PS +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL+MI+TMP+S+  FA  AQR LGTV
Sbjct: 121 PSAEFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMMIRTMPESMVEFAIIAQRHLGTV 180

Query: 181 SDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSP 240
           +DLKH+IRNC+YL+NGELGL++LA+LL+V  N T  + GSDSLL A  Y KMKK  +FS 
Sbjct: 181 NDLKHMIRNCEYLMNGELGLKRLAELLNV--NDTIFNGGSDSLLIALAYAKMKKTLKFSS 240

Query: 241 EHCDGFLYGLHYRIR 255
           E  DGFLYG +YRIR
Sbjct: 241 EITDGFLYGFYYRIR 253

BLAST of Lcy10g008000 vs. ExPASy TrEMBL
Match: A0A1S3BY95 (Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494884 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 4.1e-95
Identity = 174/255 (68.24%), Postives = 206/255 (80.78%), Query Frame = 0

Query: 1   MTLVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVN 60
           M+ VR+VW HN  QELA+LN  L +FPV+A DTEFPGFLR TPRGAP+EHFY+DLKFN+N
Sbjct: 1   MSFVRKVWYHNFIQELAILNDQLFKFPVIAMDTEFPGFLRSTPRGAPEEHFYQDLKFNLN 60

Query: 61  HLKILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKS-KGFDFNKHRNQGI 120
           HLKILQLGLTL +E+  V ++W F FSDFDE TD  SPTSIQ+LK+ KGF+F K R  GI
Sbjct: 61  HLKILQLGLTLMDENEHVGLSWVFTFSDFDEQTDFSSPTSIQYLKNKKGFEFKKQRKDGI 120

Query: 121 PSPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTV 180
           PS +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL+MI+TMP+S+  FA  AQR LGTV
Sbjct: 121 PSAEFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMMIRTMPESMVEFAIIAQRHLGTV 180

Query: 181 SDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSP 240
           +DLKH+IRNC+YL+NGELGL++LA+LL+V  N T  + GSDSLL A  Y KMKK  +FS 
Sbjct: 181 NDLKHMIRNCEYLMNGELGLKRLAELLNV--NDTIFNGGSDSLLIALAYAKMKKTLKFSS 240

Query: 241 EHCDGFLYGLHYRIR 255
           E  DGFLYG +YRIR
Sbjct: 241 EITDGFLYGFYYRIR 253

BLAST of Lcy10g008000 vs. ExPASy TrEMBL
Match: A0A0A0KTU4 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G011090 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.5e-89
Identity = 169/252 (67.06%), Postives = 198/252 (78.57%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVNHLK 63
           VRQVW HNL QELA+LN  L +FPV+  DTEFPGFLR TPRGAPQEH Y+DLKFNVNHLK
Sbjct: 5   VRQVWYHNLAQELAILNDHLFKFPVIVMDTEFPGFLRSTPRGAPQEHLYQDLKFNVNHLK 64

Query: 64  ILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLK-SKGFDFNKHRNQGIPSP 123
           ILQLGLTL +E+  V ++W F FSDFDE TDL SPTS+Q+LK +KGF+F K R  GIPS 
Sbjct: 65  ILQLGLTLMDENEHVGLSWVFTFSDFDEQTDLSSPTSMQYLKNNKGFEFKKQRKDGIPSA 124

Query: 124 QFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTVSDL 183
           +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL++IK MP+S+  FA  AQR LGTV+DL
Sbjct: 125 EFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMIIKAMPESMVEFAIIAQRFLGTVNDL 184

Query: 184 KHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSPEHC 243
           K++I +C+ L+ GELGL++LA+LLDV  N T    GSDSLL A  YVKMKK  + S E+ 
Sbjct: 185 KYMIGHCERLMKGELGLKRLAELLDV--NDTVFSGGSDSLLIALAYVKMKK-LKLSSENT 244

Query: 244 DGFLYGLHYRIR 255
            GFLYG  YRIR
Sbjct: 245 GGFLYGFQYRIR 253

BLAST of Lcy10g008000 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 9.6e-84
Identity = 166/297 (55.89%), Postives = 202/297 (68.01%), Query Frame = 0

Query: 5   RQVWSHNLPQELALLNACLSQFPVVAFDTEF----------------------------- 64
           R+VWS+NL +EL +LN  L +FP+VA DTEF                             
Sbjct: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78

Query: 65  PGFLRCTPRGAPQEHFYEDLKFNVNHLKILQLGLTLANESGDVAMTWEFNFSDFDETTDL 124
           PGFLR TP+G  +E FY+ L+FNVN L ILQLGLTLAN++G+VAMTWEFNF DFD  +D+
Sbjct: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDM 138

Query: 125 HSPTSI-QFLKSKGFDFNKHRNQGIPSPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLK 184
              +S+  FLK+KGFDF K +  GI    F   FLPIC S RI +W+TFHGFYD+AYLLK
Sbjct: 139 EGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLK 198

Query: 185 LLMIKTMPQSLAVFAAKAQRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTA 244
           LL IK +P S+A FAA AQ LLGTVSDLKH+ R CD LL+G+LGL+KLAKLLDV+R G A
Sbjct: 199 LLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIA 258

Query: 245 HHAGSDSLLTASVYVKMKKRFEFSPEHCDGFLYGLHYRIRRRPVIGNVVIPPFRQPP 272
           H AGSDSLLTA+VY KMK+ F+   E  +GFLYGL +RI+    I  +V P    PP
Sbjct: 259 HFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQAFQPIVKIVDPSAMTPP 315

BLAST of Lcy10g008000 vs. NCBI nr
Match: XP_038890962.1 (putative CCR4-associated factor 1 homolog 8 [Benincasa hispida])

HSP 1 Score: 375.2 bits (962), Expect = 5.2e-100
Identity = 180/255 (70.59%), Postives = 211/255 (82.75%), Query Frame = 0

Query: 1   MTLVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVN 60
           M LVRQVWSHNL +ELA+ N  LS+FPV+  DTEFPGF+R TPRGAP+EH Y+DLKFN+N
Sbjct: 1   MDLVRQVWSHNLYRELAIFNVYLSRFPVIVIDTEFPGFIRSTPRGAPKEHLYQDLKFNLN 60

Query: 61  HLKILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNKHRNQGIP 120
           HLKILQLGLTL +E+G+V MTWEF FSDFDE TDL +PTSIQFLK+KG DF K R +GI 
Sbjct: 61  HLKILQLGLTLVDENGNVGMTWEFTFSDFDEQTDLSNPTSIQFLKNKGLDFKKQREEGIR 120

Query: 121 SPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTVS 180
           S +F+ AFLPI CSNRI KW+TFHG YDVAYLLKL++IK+MP+S+  FA  A+  LGTVS
Sbjct: 121 SAEFQLAFLPILCSNRIVKWVTFHGIYDVAYLLKLMVIKSMPESVVEFAIIARCFLGTVS 180

Query: 181 DLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHH-AGSDSLLTASVYVKMKKRFEFSP 240
           DLK+IIRNC+YLLNGELGL+KLAKLL++  +   HH AG DSLL AS Y KMKK  EFS 
Sbjct: 181 DLKYIIRNCEYLLNGELGLKKLAKLLNIVNDIPTHHLAGFDSLLIASAYAKMKKMHEFSS 240

Query: 241 EHCDGFLYGLHYRIR 255
           E+ DGFLYGL+YRIR
Sbjct: 241 ENLDGFLYGLYYRIR 255

BLAST of Lcy10g008000 vs. NCBI nr
Match: KAA0044544.1 (putative CCR4-associated factor 1-like protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 358.6 bits (919), Expect = 5.0e-95
Identity = 175/255 (68.63%), Postives = 205/255 (80.39%), Query Frame = 0

Query: 1   MTLVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVN 60
           M+ VRQVW HNL QEL +LN  L +FPV+A DTEFPGFLR T RGAP+EHFY+DLKFN+N
Sbjct: 1   MSFVRQVWYHNLTQELVILNDQLFKFPVIAMDTEFPGFLRSTLRGAPEEHFYQDLKFNLN 60

Query: 61  HLKILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKS-KGFDFNKHRNQGI 120
           HLKILQLGLTL +E+  V ++W F FSDFDE TD  SPTSIQ+LK+ KGF+F K R  GI
Sbjct: 61  HLKILQLGLTLMDENEHVGLSWVFTFSDFDEQTDFSSPTSIQYLKNKKGFEFKKQRKDGI 120

Query: 121 PSPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTV 180
           PS +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL+MI+TMP+S+  FA  AQR LGTV
Sbjct: 121 PSAEFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMMIRTMPESMVEFAIIAQRHLGTV 180

Query: 181 SDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSP 240
           +DLKH+IRNC+YL+NGELGL++LA+LL+V  N T  + GSDSLL A  Y KMKK  +FS 
Sbjct: 181 NDLKHMIRNCEYLMNGELGLKRLAELLNV--NDTIFNGGSDSLLIALAYAKMKKTLKFSS 240

Query: 241 EHCDGFLYGLHYRIR 255
           E  DGFLYG HYRIR
Sbjct: 241 EITDGFLYGFHYRIR 253

BLAST of Lcy10g008000 vs. NCBI nr
Match: XP_008454486.1 (PREDICTED: putative CCR4-associated factor 1 homolog 8 [Cucumis melo] >TYK17039.1 putative CCR4-associated factor 1-like protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 357.8 bits (917), Expect = 8.5e-95
Identity = 174/255 (68.24%), Postives = 206/255 (80.78%), Query Frame = 0

Query: 1   MTLVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVN 60
           M+ VR+VW HN  QELA+LN  L +FPV+A DTEFPGFLR TPRGAP+EHFY+DLKFN+N
Sbjct: 1   MSFVRKVWYHNFIQELAILNDQLFKFPVIAMDTEFPGFLRSTPRGAPEEHFYQDLKFNLN 60

Query: 61  HLKILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKS-KGFDFNKHRNQGI 120
           HLKILQLGLTL +E+  V ++W F FSDFDE TD  SPTSIQ+LK+ KGF+F K R  GI
Sbjct: 61  HLKILQLGLTLMDENEHVGLSWVFTFSDFDEQTDFSSPTSIQYLKNKKGFEFKKQRKDGI 120

Query: 121 PSPQFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTV 180
           PS +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL+MI+TMP+S+  FA  AQR LGTV
Sbjct: 121 PSAEFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMMIRTMPESMVEFAIIAQRHLGTV 180

Query: 181 SDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSP 240
           +DLKH+IRNC+YL+NGELGL++LA+LL+V  N T  + GSDSLL A  Y KMKK  +FS 
Sbjct: 181 NDLKHMIRNCEYLMNGELGLKRLAELLNV--NDTIFNGGSDSLLIALAYAKMKKTLKFSS 240

Query: 241 EHCDGFLYGLHYRIR 255
           E  DGFLYG +YRIR
Sbjct: 241 EITDGFLYGFYYRIR 253

BLAST of Lcy10g008000 vs. NCBI nr
Match: XP_004151393.1 (putative CCR4-associated factor 1 homolog 8 [Cucumis sativus])

HSP 1 Score: 339.3 bits (869), Expect = 3.1e-89
Identity = 169/252 (67.06%), Postives = 198/252 (78.57%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVNHLK 63
           VRQVW HNL QELA+LN  L +FPV+  DTEFPGFLR TPRGAPQEH Y+DLKFNVNHLK
Sbjct: 5   VRQVWYHNLAQELAILNDHLFKFPVIVMDTEFPGFLRSTPRGAPQEHLYQDLKFNVNHLK 64

Query: 64  ILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLK-SKGFDFNKHRNQGIPSP 123
           ILQLGLTL +E+  V ++W F FSDFDE TDL SPTS+Q+LK +KGF+F K R  GIPS 
Sbjct: 65  ILQLGLTLMDENEHVGLSWVFTFSDFDEQTDLSSPTSMQYLKNNKGFEFKKQRKDGIPSA 124

Query: 124 QFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTVSDL 183
           +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL++IK MP+S+  FA  AQR LGTV+DL
Sbjct: 125 EFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMIIKAMPESMVEFAIIAQRFLGTVNDL 184

Query: 184 KHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSPEHC 243
           K++I +C+ L+ GELGL++LA+LLDV  N T    GSDSLL A  YVKMKK  + S E+ 
Sbjct: 185 KYMIGHCERLMKGELGLKRLAELLDV--NDTVFSGGSDSLLIALAYVKMKK-LKLSSENT 244

Query: 244 DGFLYGLHYRIR 255
            GFLYG  YRIR
Sbjct: 245 GGFLYGFQYRIR 253

BLAST of Lcy10g008000 vs. NCBI nr
Match: KAE8649090.1 (hypothetical protein Csa_014788 [Cucumis sativus])

HSP 1 Score: 328.6 bits (841), Expect = 5.5e-86
Identity = 164/245 (66.94%), Postives = 193/245 (78.78%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVNHLK 63
           VRQVW HNL QELA+LN  L +FPV+  DTEFPGFLR TPRGAPQEH Y+DLKFNVNHLK
Sbjct: 5   VRQVWYHNLAQELAILNDHLFKFPVIVMDTEFPGFLRSTPRGAPQEHLYQDLKFNVNHLK 64

Query: 64  ILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLK-SKGFDFNKHRNQGIPSP 123
           ILQLGLTL +E+  V ++W F FSDFDE TDL SPTS+Q+LK +KGF+F K R  GIPS 
Sbjct: 65  ILQLGLTLMDENEHVGLSWVFTFSDFDEQTDLSSPTSMQYLKNNKGFEFKKQRKDGIPSA 124

Query: 124 QFRRAFLPICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGTVSDL 183
           +FRRAFLPI  SNRI KW+TFHG YDVAYLLKL++IK MP+S+  FA  AQR LGTV+DL
Sbjct: 125 EFRRAFLPIFSSNRITKWITFHGIYDVAYLLKLMIIKAMPESMVEFAIIAQRFLGTVNDL 184

Query: 184 KHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFSPEHC 243
           K++I +C+ L+ GELGL++LA+LLDV  N T    GSDSLL A  YVKMKK  + S E+ 
Sbjct: 185 KYMIGHCERLMKGELGLKRLAELLDV--NDTVFSGGSDSLLIALAYVKMKK-LKLSSENT 244

Query: 244 DGFLY 248
            GFLY
Sbjct: 245 GGFLY 246

BLAST of Lcy10g008000 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 4.9e-48
Identity = 107/259 (41.31%), Postives = 148/259 (57.14%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRG---APQEHFYEDLKFNVN 63
           +R+VW+ NL  E+AL+   +  FP VA DTEFPG + C P G      E+ YE LK NVN
Sbjct: 12  IREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIV-CRPVGTFKTNTEYHYETLKTNVN 71

Query: 64  HLKILQLGLTLANESGDVA--------MTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFN 123
            LK++QLGLT ++E G++           W+FNF +FD  +D+++  SI+ L+  G DF 
Sbjct: 72  ILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIELLRQSGIDFV 131

Query: 124 KHRNQGIPSPQFRRAFLPI-CCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAK 183
           K+   GI S +F    +      N    W+TFH  YD  YLLKLL  + +P++   F   
Sbjct: 132 KNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGFFEM 191

Query: 184 AQRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKM 243
                  V D+KH+++ C+ L     GL KLA+LLDVER G  H AGSDSLLT+  + K+
Sbjct: 192 ISVYFPRVYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTFRKL 251

Query: 244 KKRFEF-SPEHCDGFLYGL 250
           ++ F   S E   G LYGL
Sbjct: 252 QENFFIGSMEKYSGVLYGL 266

BLAST of Lcy10g008000 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.2e-47
Identity = 108/258 (41.86%), Postives = 145/258 (56.20%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRG---APQEHFYEDLKFNVN 63
           +R+VW+ NL +E+ L+   +  FP VA DTEFPG +   P G   +  ++ YE LK NVN
Sbjct: 12  IREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVN 71

Query: 64  HLKILQLGLTLANESG-------DVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNK 123
            LK++QLGLT +NE G       D    W+FNF +FD  +D+ +  SI+ LK  G D  K
Sbjct: 72  ILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIELLKQSGIDLAK 131

Query: 124 HRNQGIPSPQFRRAFLPI-CCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKA 183
           +   GI S +F    +      N    W+TFH  YD  YLLKLL  + +P S   F    
Sbjct: 132 NTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLI 191

Query: 184 QRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMK 243
                TV D+KH+++ C+ L     GL KLA+LL+VER G  H AGSDSLLT+  + K+K
Sbjct: 192 NVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLK 251

Query: 244 KRFEFSPEH-CDGFLYGL 250
           + F   P H   G LYGL
Sbjct: 252 ENFFVGPLHKYSGVLYGL 265

BLAST of Lcy10g008000 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.2e-47
Identity = 108/258 (41.86%), Postives = 145/258 (56.20%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRG---APQEHFYEDLKFNVN 63
           +R+VW+ NL +E+ L+   +  FP VA DTEFPG +   P G   +  ++ YE LK NVN
Sbjct: 12  IREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVN 71

Query: 64  HLKILQLGLTLANESG-------DVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNK 123
            LK++QLGLT +NE G       D    W+FNF +FD  +D+ +  SI+ LK  G D  K
Sbjct: 72  ILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIELLKQSGIDLAK 131

Query: 124 HRNQGIPSPQFRRAFLPI-CCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKA 183
           +   GI S +F    +      N    W+TFH  YD  YLLKLL  + +P S   F    
Sbjct: 132 NTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLI 191

Query: 184 QRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMK 243
                TV D+KH+++ C+ L     GL KLA+LL+VER G  H AGSDSLLT+  + K+K
Sbjct: 192 NVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLK 251

Query: 244 KRFEFSPEH-CDGFLYGL 250
           + F   P H   G LYGL
Sbjct: 252 ENFFVGPLHKYSGVLYGL 265

BLAST of Lcy10g008000 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.2e-47
Identity = 108/258 (41.86%), Postives = 145/258 (56.20%), Query Frame = 0

Query: 4   VRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRG---APQEHFYEDLKFNVN 63
           +R+VW+ NL +E+ L+   +  FP VA DTEFPG +   P G   +  ++ YE LK NVN
Sbjct: 12  IREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVN 71

Query: 64  HLKILQLGLTLANESG-------DVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNK 123
            LK++QLGLT +NE G       D    W+FNF +FD  +D+ +  SI+ LK  G D  K
Sbjct: 72  ILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIELLKQSGIDLAK 131

Query: 124 HRNQGIPSPQFRRAFLPI-CCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKA 183
           +   GI S +F    +      N    W+TFH  YD  YLLKLL  + +P S   F    
Sbjct: 132 NTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLI 191

Query: 184 QRLLGTVSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMK 243
                TV D+KH+++ C+ L     GL KLA+LL+VER G  H AGSDSLLT+  + K+K
Sbjct: 192 NVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLK 251

Query: 244 KRFEFSPEH-CDGFLYGL 250
           + F   P H   G LYGL
Sbjct: 252 ENFFVGPLHKYSGVLYGL 265

BLAST of Lcy10g008000 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.6e-46
Identity = 97/251 (38.65%), Postives = 147/251 (58.57%), Query Frame = 0

Query: 3   LVRQVWSHNLPQELALLNACLSQFPVVAFDTEFPGFLRCTPRGAPQEHFYEDLKFNVNHL 62
           L R+VW  N+ +E+A +  CL +FP++AFDTE+PG +  T   +  +  Y  +K NV + 
Sbjct: 8   LARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENT 67

Query: 63  KILQLGLTLANESGDVAMTWEFNFSDFDETTDLHSPTSIQFLKSKGFDFNKHRNQGIPSP 122
           K++Q G TL N  G++   WE NFS+F + +D  +  SI+FL+  G D  K R++G+   
Sbjct: 68  KLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGV--D 127

Query: 123 QFRRAFLP----ICCSNRIAKWLTFHGFYDVAYLLKLLMIKTMPQSLAVFAAKAQRLLGT 182
            F   F P    +  S +  +++TF G YD AY L +L    +P++   FA +  ++ G 
Sbjct: 128 MFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGKLPETHGEFATEVVKVFGQ 187

Query: 183 VSDLKHIIRNCDYLLNGELGLEKLAKLLDVERNGTAHHAGSDSLLTASVYVKMKKRFEFS 242
           V D K +   C+  L   LGL KLA+LL + R G AHHAGSDSL+TA V++K+K  +E S
Sbjct: 188 VYDTKVMAGFCEG-LGEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYEDS 247

Query: 243 PEHCDGFLYGL 250
                G +YG+
Sbjct: 248 -RFARGLIYGI 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKZ26.9e-4741.31Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SAI24.5e-4641.86Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SHJ02.2e-4538.65Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9S9P21.1e-4438.64Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
O647739.6e-4142.11Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A5A7TN622.4e-9568.63Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A5D3D3334.1e-9568.24Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BY954.1e-9568.24Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494884 PE=3 SV=1[more]
A0A0A0KTU41.5e-8967.06Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G011090 PE=3 SV... [more]
A0A6J1DLA59.6e-8455.89Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=... [more]
Match NameE-valueIdentityDescription
XP_038890962.15.2e-10070.59putative CCR4-associated factor 1 homolog 8 [Benincasa hispida][more]
KAA0044544.15.0e-9568.63putative CCR4-associated factor 1-like protein 8 [Cucumis melo var. makuwa][more]
XP_008454486.18.5e-9568.24PREDICTED: putative CCR4-associated factor 1 homolog 8 [Cucumis melo] >TYK17039.... [more]
XP_004151393.13.1e-8967.06putative CCR4-associated factor 1 homolog 8 [Cucumis sativus][more]
KAE8649090.15.5e-8666.94hypothetical protein Csa_014788 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G32070.14.9e-4841.31Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.13.2e-4741.86Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.23.2e-4741.86Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.33.2e-4741.86Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G06450.11.6e-4638.65Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 7..120
e-value: 3.9E-12
score: 46.1
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 139..227
e-value: 1.0E-5
score: 25.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..252
e-value: 2.9E-76
score: 258.1
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 3..256
NoneNo IPR availablePANTHERPTHR10797:SF57CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATEDcoord: 3..256
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 2..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g008000.1Lcy10g008000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043928 exonucleolytic catabolism of deadenylated mRNA
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030015 CCR4-NOT core complex
cellular_component GO:0005634 nucleus
cellular_component GO:0000932 P-body
cellular_component GO:0030014 CCR4-NOT complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity