Lcy10g007310 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy10g007310
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	sterol 14-demethylase-like
Location	Chr10: 30511826 .. 30513711 (-)
RNA-Seq Expression	Lcy10g007310
Synteny	Lcy10g007310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCCGGAGAACAAGTTATTCAATGTGGGTCTCCTCGTAGTGGCGACTTTAGTCCTCGCCAAGGTCATTTCTGCTGTTCTGAATTCCAGATCTAAGAAACGGGCTCCTCCCACTGTCAAGGGGCTGCCCCTTGTTGGGGGGTTGCTCCGCTTTCTTAAGGGTCCGATCGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACTTATTGCACAAGAAGATTACCTTCTTGATTGGGCCTGAGGTTTCTGCGCATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGGCCTGGCGTTGTGTTTGATGTGGATTACTCCGTCCGCCAAGAGCAGTTCAGGTTCTTCACTGAAGCTCTCAGAGTCACGAAATTGAAAGGCTATGTGGATCAGATGGTTAAAGAGGCAGAGGTCAGTATCAACCCCCTTGAAATATCTAAATGTATAAGATATCATTAAATGCAGCCTCAGATAAAGAATGTGGATATTAGGATTCTGTGAGAACTTGTACTCTGCTTGTTAACTTTTAAGTTTTACTAAATTTTTGGAAAAGTTTCTCCCTCTCTCTAGCTGTAATGATTGATGCGATGTTGATGAAAAATGAATTATAAAGAAGTAAAATGCAGCCCAGCATCCTTGCTAATGGGAATGCATGTTAAATGTTAACAACAGTCCTTAGAGATTTGTTTTCTTAATGTATAGTCTGAACTTCCATGTATCTCTTAAAACGTCTAGCTCCTGATGCTTGTGTTCAAATCTTTGAGTCACATTCTCTTCGTTTTACAGTATTCTATAAATCTGCTTAACCTTTGGGATGTGATGGGGTATTTGAGCAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTCCTTGGTAGAGAGGTTCGTGATAAGCTTTTTGCTGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCGGCTCATCGTCGTCGCGATCAAGCCCGCAGCAAGCTTGCAGATATCTTTGCGAAAATCATAGCCTCCAGAAAATGCAATGGAAGTTCAGAAAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACAACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCATATCTCCTACGCCACAAAGAGTACATGTCTGCTGTTGTAGAGGAGCAGCAAAAACTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGACAACCTCCATAGATGCATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTCAGTGTGACAACTAGAGAGGGGAAAGAATTTGTTATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTTGCTAATCGACTTCCTCATGTCTACAACGATCCAGACAGGTATGATCCAGACAGGTTTGCCCCTGGAAGAGATGAAGACAAGGCAGCTGGGCAATTCTCCTACATCTCATTCGGAGGCGGCAGGCACGGGTGCCTTGGCGAGCCCTTTGCTTACTTACAGATAAAAGCAATATGGAGTCAGTTACTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTAGGTGTTAAAGGGAGAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGTTAA

mRNA sequence

ATGGAGCCGGAGAACAAGTTATTCAATGTGGGTCTCCTCGTAGTGGCGACTTTAGTCCTCGCCAAGGTCATTTCTGCTGTTCTGAATTCCAGATCTAAGAAACGGGCTCCTCCCACTGTCAAGGGGCTGCCCCTTGTTGGGGGGTTGCTCCGCTTTCTTAAGGGTCCGATCGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACTTATTGCACAAGAAGATTACCTTCTTGATTGGGCCTGAGGTTTCTGCGCATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGGCCTGGCGTTGTGTTTGATGTGGATTACTCCGTCCGCCAAGAGCAGTTCAGGTTCTTCACTGAAGCTCTCAGAGTCACGAAATTGAAAGGCTATGTGGATCAGATGGTTAAAGAGGCAGAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTCCTTGGTAGAGAGGTTCGTGATAAGCTTTTTGCTGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCGGCTCATCGTCGTCGCGATCAAGCCCGCAGCAAGCTTGCAGATATCTTTGCGAAAATCATAGCCTCCAGAAAATGCAATGGAAGTTCAGAAAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACAACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCATATCTCCTACGCCACAAAGAGTACATGTCTGCTGTTGTAGAGGAGCAGCAAAAACTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGACAACCTCCATAGATGCATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTCAGTGTGACAACTAGAGAGGGGAAAGAATTTGTTATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTTGCTAATCGACTTCCTCATGTCTACAACGATCCAGACAGGTATGATCCAGACAGGTTTGCCCCTGGAAGAGATGAAGACAAGGCAGCTGGGCAATTCTCCTACATCTCATTCGGAGGCGGCAGGCACGGGTGCCTTGGCGAGCCCTTTGCTTACTTACAGATAAAAGCAATATGGAGTCAGTTACTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTAGGTGTTAAAGGGAGAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGTTAA

Coding sequence (CDS)

ATGGAGCCGGAGAACAAGTTATTCAATGTGGGTCTCCTCGTAGTGGCGACTTTAGTCCTCGCCAAGGTCATTTCTGCTGTTCTGAATTCCAGATCTAAGAAACGGGCTCCTCCCACTGTCAAGGGGCTGCCCCTTGTTGGGGGGTTGCTCCGCTTTCTTAAGGGTCCGATCGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACTTATTGCACAAGAAGATTACCTTCTTGATTGGGCCTGAGGTTTCTGCGCATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGGCCTGGCGTTGTGTTTGATGTGGATTACTCCGTCCGCCAAGAGCAGTTCAGGTTCTTCACTGAAGCTCTCAGAGTCACGAAATTGAAAGGCTATGTGGATCAGATGGTTAAAGAGGCAGAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTCCTTGGTAGAGAGGTTCGTGATAAGCTTTTTGCTGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCGGCTCATCGTCGTCGCGATCAAGCCCGCAGCAAGCTTGCAGATATCTTTGCGAAAATCATAGCCTCCAGAAAATGCAATGGAAGTTCAGAAAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACAACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCATATCTCCTACGCCACAAAGAGTACATGTCTGCTGTTGTAGAGGAGCAGCAAAAACTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGACAACCTCCATAGATGCATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTCAGTGTGACAACTAGAGAGGGGAAAGAATTTGTTATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTTGCTAATCGACTTCCTCATGTCTACAACGATCCAGACAGGTATGATCCAGACAGGTTTGCCCCTGGAAGAGATGAAGACAAGGCAGCTGGGCAATTCTCCTACATCTCATTCGGAGGCGGCAGGCACGGGTGCCTTGGCGAGCCCTTTGCTTACTTACAGATAAAAGCAATATGGAGTCAGTTACTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTAGGTGTTAAAGGGAGAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGTTAA

Protein sequence

MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKRRKLSVS

Homology

[Close]

BLAST of Lcy10g007310 vs. ExPASy Swiss-Prot
Match: Q9SAA9 (Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 2.5e-238
Identity = 402/486 (82.72%), Postives = 447/486 (91.98%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRS-KKRAPPTVKGL-PLVGGLLRFLKGPIV 60
           ++ ENKL   GL++VATLV+AK+I +   S S KKR PPT+K   PLVG L++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 61  MLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 120
           MLREEYPKLGSVFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 121 FDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIIL 180
           FDVDYSVRQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 181 TASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFA 240
           TASRCLLGREVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 241 KIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGA 300
           KII SRK +G +ENDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 301 YLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSH 360
           YL+R+KEY SA ++EQ+ L+ KHGDKIDHDILSEMD L+RCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 361 TDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFS 420
           +DFSVT R+GK + IPKGHI+ATSPAFANRLPH++ DPD YDP+RF+PGR+EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 421 YISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRY 480
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 481 KRRKLS 485
           KRR+LS
Sbjct: 483 KRRQLS 488

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BLAST of Lcy10g007310 vs. ExPASy Swiss-Prot
Match: P93846 (Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 1.4e-222
Identity = 367/484 (75.83%), Postives = 429/484 (88.64%), Query Frame = 0


Query: 3   PENKLFNVGL-LVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVMLR 62
           P+ +    GL LVVAT++  K++ +  +   KKR PPT+ G P+VGGL++F++GPI M+R
Sbjct: 7   PQQQRLMAGLALVVATVIFLKLLLSFRSGGGKKRLPPTIPGAPVVGGLVKFMRGPIPMIR 66

Query: 63  EEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDV 122
           E+Y  LGSVFT+ ++ ++ITFLIGPEVSAHFFK +E+++SQQEVY+FNVPTFGPGVVFDV
Sbjct: 67  EQYAALGSVFTVPIITRRITFLIGPEVSAHFFKGNEAEMSQQEVYRFNVPTFGPGVVFDV 126

Query: 123 DYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTAS 182
           DYSVRQEQFRFFTEALR  KL+ YVDQMV EAE++FSKWG+SG VDLKYELEHLIILTAS
Sbjct: 127 DYSVRQEQFRFFTEALRANKLRSYVDQMVAEAEEYFSKWGESGTVDLKYELEHLIILTAS 186

Query: 183 RCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKII 242
           RCLLGREVR+KLF DVSALFHDLDNG+ PISV+FPYLPIPAH+RRD+AR++LA+IFA II
Sbjct: 187 RCLLGREVREKLFDDVSALFHDLDNGIQPISVLFPYLPIPAHKRRDKARARLAEIFATII 246

Query: 243 ASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLL 302
            SRK +G SE DMLQCFIDSKYK+GRPTT+ EVTGLLIAALFAGQHTSSITSTWTGAY+L
Sbjct: 247 KSRKASGQSEEDMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYML 306

Query: 303 RHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDF 362
           R K+Y +  VEEQ+ +M +HGDKIDHDIL+EMD L+RCIKEALRLHPPLIML+R SH+DF
Sbjct: 307 RFKQYFAEAVEEQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDF 366

Query: 363 SVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYIS 422
           +VTT+EGKE+ IPKGHI+ATSP+FANRLPH+Y +PD YDPDRF PGR+EDKAAG FSYIS
Sbjct: 367 TVTTKEGKEYDIPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYIS 426

Query: 423 FGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKRR 482
           FGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL+SPFPE DWNAMVVG+KG VMV YKRR
Sbjct: 427 FGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRR 486

Query: 483 KLSV 486
           KL V
Sbjct: 487 KLVV 490

[Close]

BLAST of Lcy10g007310 vs. ExPASy Swiss-Prot
Match: P93596 (Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP51 PE=2 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 1.1e-219
Identity = 363/451 (80.49%), Postives = 410/451 (90.91%), Query Frame = 0


Query: 35  RAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFK 94
           R PPT+ G P+VGGLLRFL+GPI ++R EY +LG VFT+ +L ++ITFLIGP+VSAHFFK
Sbjct: 1   RPPPTIPGAPVVGGLLRFLRGPIPLIRAEYARLGPVFTVPILTRRITFLIGPDVSAHFFK 60

Query: 95  ASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAE 154
           ++ESD+SQQEVY+FNVPTFGPGVVFDVDY VRQEQFRFFTEALR  KL+ YVDQMV EAE
Sbjct: 61  SNESDMSQQEVYRFNVPTFGPGVVFDVDYQVRQEQFRFFTEALRANKLRSYVDQMVAEAE 120

Query: 155 DFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVM 214
           ++FSKWG+SG VDLKYELEHLIILTASRCLLGREVR+KLF DVSALFHDLDNGMLPISV+
Sbjct: 121 EYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREKLFDDVSALFHDLDNGMLPISVI 180

Query: 215 FPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEV 274
           FPYLPIPAHRRRDQAR++LA+IFA II SRK +G SE DMLQCFIDSKYK+GR TT+SEV
Sbjct: 181 FPYLPIPAHRRRDQARTRLAEIFATIIKSRKASGQSEEDMLQCFIDSKYKNGRQTTESEV 240

Query: 275 TGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMD 334
           TGLLIAALFAGQHTSSITSTWTGAYLL+ ++Y +  VEEQ+++M +HGDKIDHDIL+EMD
Sbjct: 241 TGLLIAALFAGQHTSSITSTWTGAYLLKFQQYFAEAVEEQKEVMKRHGDKIDHDILAEMD 300

Query: 335 NLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYN 394
            L+RCIKEALRLHPPLIML+R SH+DFSVTTREGKEF IPKGHI+ATSPAFANRLPH++ 
Sbjct: 301 VLYRCIKEALRLHPPLIMLLRQSHSDFSVTTREGKEFDIPKGHIVATSPAFANRLPHIFK 360

Query: 395 DPDRYDPDRFAPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELEL 454
           +PD YDPDRFA GR+EDK AG FSYISFGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL
Sbjct: 361 NPDSYDPDRFAAGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFEL 420

Query: 455 ISPFPEIDWNAMVVGVKGRVMVRYKRRKLSV 486
           +SPFPE DWNAMVVG+KG VMV YKRRKL V
Sbjct: 421 VSPFPENDWNAMVVGIKGEVMVNYKRRKLIV 451

[Close]

BLAST of Lcy10g007310 vs. ExPASy Swiss-Prot
Match: Q1ZXH9 (Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN=cyp51 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.8e-103
Identity = 205/478 (42.89%), Postives = 293/478 (61.30%), Query Frame = 0


Query: 10  VGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGS 69
           + +LV+A LV+          +S    PP V+ +P +G   +F K P+ ++R  Y +LG 
Sbjct: 5   IAVLVIAILVIFAF------KKSPSNIPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGE 64

Query: 70  VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQ 129
           +FTL+L+  K+TF++GPE  A FF+ ++ +LS +E Y+F  P FG GVV+D +  +  EQ
Sbjct: 65  IFTLHLMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQ 124

Query: 130 FRFFTEALRVTKLKGYVDQMVKEAEDFF-SKWGDSGEVDLKYELEHLIILTASRCLLGRE 189
            RF    L +++LK  V  + +E E +F +KWGDSGE+DL YE+  L ILTASRCL+G+ 
Sbjct: 125 LRFVKNGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKS 184

Query: 190 VRDKL--FADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIAS-RK 249
           +   L     ++ L+H+L+ G+ PIS  FP LP+P+ ++RD AR+K+A IF  II   R+
Sbjct: 185 INKSLGQSGQLADLYHELEEGLNPISFFFPNLPLPSFKKRDAARAKVAAIFHSIIQERRR 244

Query: 250 CNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKE 309
               S +D+L   ++SKYKDG    D ++ GL+I  LFAGQHTSSIT T+T  YLL + E
Sbjct: 245 STDDSVDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLE 304

Query: 310 YMSAVVEEQQKLMGKHGD-KIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVT 369
           Y     ++   ++ K    +I+ D L  M+ L   I+E LRLHPPLI LMR       +T
Sbjct: 305 YFDETQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRK-----VMT 364

Query: 370 TREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYISFGG 429
             E K   IP GHI+A SP    RLP VY +PD ++P RF     EDK    FS+I+FGG
Sbjct: 365 PMEYKGKTIPAGHILAVSPQVGMRLPTVYKNPDSFEPKRFDV---EDKT--PFSFIAFGG 424

Query: 430 GRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKRRK 483
           G+HGC GE F  LQIK IW+ L   + LE + P P  D+ ++V G KG  MV+Y +++
Sbjct: 425 GKHGCPGENFGILQIKTIWTVLSTKYNLE-VGPVPPTDFTSLVAGPKGPCMVKYSKKQ 465

[Close]

BLAST of Lcy10g007310 vs. ExPASy Swiss-Prot
Match: Q4PJW3 (Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 5.9e-91
Identity = 179/451 (39.69%), Postives = 264/451 (58.54%), Query Frame = 0


Query: 34  KRAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFF 93
           K  P  V  +P +G  + F K PI  L + Y K G VF+  ++ K  T+L+G E +A  F
Sbjct: 58  KSPPYIVSPIPFLGHAIAFGKSPIEFLEDAYEKYGPVFSFTMVGKTFTYLLGSEAAALLF 117

Query: 94  KASESDLSQQEVY-QFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKE 153
            +   DL+ +EVY +   P FG GV +DV  +V  EQ +     L +   + +V  + KE
Sbjct: 118 NSKNEDLNAEEVYSRLTTPVFGKGVAYDVPNTVFLEQKKMLKSGLNIAHFRQHVSIIEKE 177

Query: 154 AEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPIS 213
            +++F  WG+SGE +L   L  LIILTAS CL G+E+R +L   V+ L+ DLD G    +
Sbjct: 178 TKEYFKSWGESGEKNLFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAA 237

Query: 214 VMFP-YLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPTTD 273
            + P +LP+P+ RRRD+A  ++ +IF K I  R+ +G   +D+LQ  ++S YKDGRP TD
Sbjct: 238 WLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRRESGEKIDDILQTLLESTYKDGRPLTD 297

Query: 274 SEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILS 333
            EV G+LI  L AGQHTSS TS W G +L R K        EQ+ + G++   + +D L 
Sbjct: 298 DEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCFLEQKTVCGENLPPLTYDQLK 357

Query: 334 EMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPH 393
           +++ L RCIKE LRL PP++ +MR + T  +V       + IP GH +  SP    RL  
Sbjct: 358 DLNLLDRCIKETLRLRPPIMTMMRLAKTPLTVA-----GYTIPPGHQVCVSPTVNQRLKD 417

Query: 394 VYNDPDRYDPDRFAPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFE 453
            + +   ++PDR+    D   +  +F+Y+ FG GRH C+GE FAY+QIK IWS +LR +E
Sbjct: 418 SWVERLDFNPDRYL--EDSPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYE 477

Query: 454 LELISP-FPEIDWNAMVVGVKGRVMVRYKRR 482
            +LI   FP +++  M +    + ++RYKRR
Sbjct: 478 FDLIDGYFPTVNYTTM-IHTPEKPIIRYKRR 500

[Close]

BLAST of Lcy10g007310 vs. ExPASy TrEMBL
Match: A0A6J1F2S4 (sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111439144 PE=3 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 1.6e-269
Identity = 460/485 (94.85%), Postives = 476/485 (98.14%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG++RFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQKLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF+P RDEDKAAG FSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSV 486
           RKLSV
Sbjct: 481 RKLSV 485

[Close]

BLAST of Lcy10g007310 vs. ExPASy TrEMBL
Match: A0A6J1J0F8 (sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 3.6e-269
Identity = 462/486 (95.06%), Postives = 476/486 (97.94%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK  NVGLLVVATL+LAKVISAVLNSRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK +GSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRFAPGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVS 487
           RKLSV+
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Lcy10g007310 vs. ExPASy TrEMBL
Match: A0A6J1I3Z5 (sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111470769 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.0e-268
Identity = 458/485 (94.43%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG+LRFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMLRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVF LNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFILNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQ+KLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHT+
Sbjct: 301 LRHKEYMSAVIEEQKKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTN 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSV T+EGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRF+P RDEDKAAG FSYI
Sbjct: 361 FSVITKEGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSV 486
           RKLSV
Sbjct: 481 RKLSV 485

[Close]

BLAST of Lcy10g007310 vs. ExPASy TrEMBL
Match: A0A6J1GUN6 (sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.3e-268
Identity = 460/486 (94.65%), Postives = 475/486 (97.74%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK  NVGLLVVATL+LAKVISAVLNSRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           R+EYP+LG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK +GSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRFAPGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVS 487
           RKLSV+
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Lcy10g007310 vs. ExPASy TrEMBL
Match: A0A0A0KYP3 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.5e-267
Identity = 459/487 (94.25%), Postives = 476/487 (97.74%), Query Frame = 0


Query: 1   MEPEN-KLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVM 60
           MEP+N K FNVGLLV+ATL+LAK+ISA+LNSRS KR PPTVKG+P +GGLLRFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LR+EYPKLGSVFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYS+RQEQFRFFTEALRVTKLKGYVDQMV+EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHT 360
           LL HKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSY 420
           DFSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRFAPGRDEDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGAFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG+VMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 487
           RRKLSVS
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of Lcy10g007310 vs. NCBI nr
Match: XP_022932660.1 (sterol 14-demethylase-like [Cucurbita moschata] >XP_022932661.1 sterol 14-demethylase-like [Cucurbita moschata] >XP_022932662.1 sterol 14-demethylase-like [Cucurbita moschata] >KAG7029139.1 Sterol 14-demethylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 937.9 bits (2423), Expect = 3.4e-269
Identity = 460/485 (94.85%), Postives = 476/485 (98.14%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG++RFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQKLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF+P RDEDKAAG FSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSV 486
           RKLSV
Sbjct: 481 RKLSV 485

[Close]

BLAST of Lcy10g007310 vs. NCBI nr
Match: KAG6597695.1 (Sterol 14-demethylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 937.9 bits (2423), Expect = 3.4e-269
Identity = 460/485 (94.85%), Postives = 476/485 (98.14%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG++RFLKGPIVML
Sbjct: 83  MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 142

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 143 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 202

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 203 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 262

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 263 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 322

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 323 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 382

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQKLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 383 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 442

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF+P RDEDKAAG FSYI
Sbjct: 443 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 502

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 503 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 562

Query: 481 RKLSV 486
           RKLSV
Sbjct: 563 RKLSV 567

[Close]

BLAST of Lcy10g007310 vs. NCBI nr
Match: XP_022980879.1 (sterol 14-demethylase-like [Cucurbita maxima])

HSP 1 Score: 936.8 bits (2420), Expect = 7.5e-269
Identity = 462/486 (95.06%), Postives = 476/486 (97.94%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK  NVGLLVVATL+LAKVISAVLNSRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK +GSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRFAPGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVS 487
           RKLSV+
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Lcy10g007310 vs. NCBI nr
Match: XP_023527569.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.4 bits (2419), Expect = 9.8e-269
Identity = 462/486 (95.06%), Postives = 476/486 (97.94%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK  NVGLLVVATL+LAKVISAVLNSRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK +GSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRFAPGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVS 487
           RKLSV+
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Lcy10g007310 vs. NCBI nr
Match: XP_023539138.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.4 bits (2419), Expect = 9.8e-269
Identity = 460/485 (94.85%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 60
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRS KRAPPTV+GLPLVGG++RFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSNKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQKLM KHGDK DHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYI 420
           FSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF+P RDEDKAAG FSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKG+VMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSV 486
           RKLSV
Sbjct: 481 RKLSV 485

[Close]

BLAST of Lcy10g007310 vs. TAIR 10
Match: AT1G11680.1 (CYTOCHROME P450 51G1 )

HSP 1 Score: 825.9 bits (2132), Expect = 1.7e-239
Identity = 402/486 (82.72%), Postives = 447/486 (91.98%), Query Frame = 0


Query: 1   MEPENKLFNVGLLVVATLVLAKVISAVLNSRS-KKRAPPTVKGL-PLVGGLLRFLKGPIV 60
           ++ ENKL   GL++VATLV+AK+I +   S S KKR PPT+K   PLVG L++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 61  MLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 120
           MLREEYPKLGSVFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 121 FDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIIL 180
           FDVDYSVRQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 181 TASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFA 240
           TASRCLLGREVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 241 KIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGA 300
           KII SRK +G +ENDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 301 YLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSH 360
           YL+R+KEY SA ++EQ+ L+ KHGDKIDHDILSEMD L+RCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 361 TDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFAPGRDEDKAAGQFS 420
           +DFSVT R+GK + IPKGHI+ATSPAFANRLPH++ DPD YDP+RF+PGR+EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 421 YISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGRVMVRY 480
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 481 KRRKLS 485
           KRR+LS
Sbjct: 483 KRRQLS 488

[Close]

BLAST of Lcy10g007310 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 123.6 bits (309), Expect = 4.3e-28
Identity = 114/465 (24.52%), Postives = 199/465 (42.80%), Query Frame = 0


Query: 18  LVLAKVISAVLNSRSKK-RAPPTVKGLPLVGGLLR-FLKGPIVMLREEYPKLGSVFTLNL 77
           L L + I+ V  S S K   PP   G P VG   + + + P V    +  + GSVF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 78  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTE 137
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 138 ALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF- 197
           A     ++  V  +   A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 198 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSE 257
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ N SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 258 NDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVV 317
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 318 EEQQKLM--GKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGK 377
           EEQ  +    K G+ +  +   +M   +R I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 378 EFVIPKGHIIATSPAFAN--RLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYISFGGGRH 437
            ++IPKG  +   P F N      +++DP ++DP RF      + A    +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 436

Query: 438 GCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG 473
            C G   A L+I  +   L   +   ++ P   I +    +   G
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNG 454

[Close]

BLAST of Lcy10g007310 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 119.4 bits (298), Expect = 8.0e-27
Identity = 112/445 (25.17%), Postives = 193/445 (43.37%), Query Frame = 0


Query: 18  LVLAKVISAVLNSRSKK-RAPPTVKGLPLVGGLLR-FLKGPIVMLREEYPKLGSVFTLNL 77
           L L + I+ V  S S K   PP   G P VG   + + + P V    +  + GSVF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 78  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTE 137
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 138 ALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF- 197
           A     ++  V  +   A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 198 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSE 257
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ N SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 258 NDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVV 317
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 318 EEQQKLM--GKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGK 377
           EEQ  +    K G+ +  +   +M   +R I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 378 EFVIPKGHIIATSPAFAN--RLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYISFGGGRH 437
            ++IPKG  +   P F N      +++DP ++DP RF      + A    +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 434

Query: 438 GCLGEPFAYLQIKAIWSQLLRNFEL 453
            C G   A L+I  +   L   + L
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRL 434

[Close]

BLAST of Lcy10g007310 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 118.2 bits (295), Expect = 1.8e-26
Identity = 105/460 (22.83%), Postives = 195/460 (42.39%), Query Frame = 0


Query: 32  SKKRAPPTVKGLPLVGGLLR-FLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSA 91
           SK   PP   G P VG   + + + P V  + +  + GSVF  ++L      +  PE +A
Sbjct: 32  SKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCVMISSPE-AA 91

Query: 92  HFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMV 151
            F   ++S L +           G   +F        +  +    A     ++  V  + 
Sbjct: 92  KFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIE 151

Query: 152 KEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF-ADVSALFHDLDNGML 211
             A+D    W +   ++   E++      A   + G++  + L+  D+   ++ L+ G  
Sbjct: 152 SIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFGKD--EVLYREDLKRCYYILEKGYN 211

Query: 212 PISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPT 271
            + V    LP     +  +AR +L+ I A+I++ R+ NGSS ND+L  F+  K       
Sbjct: 212 SMPV---NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMGDK----EEL 271

Query: 272 TDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLM--GKHGDKIDH 331
           TD ++   +I  +FA + T++   +W   YL  +   + AV EEQ  +    + G+ +  
Sbjct: 272 TDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTW 331

Query: 332 DILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFAN 391
               +M    R I+E LR+   L    R +  D      E + ++IPKG  +   P F N
Sbjct: 332 GDTKKMPLTSRVIQETLRVASILSFTFREAVEDV-----EYEGYLIPKGWKVL--PLFRN 391

Query: 392 --RLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQ 451
                 ++++P ++DP RF      + A    +++ FG G H C G   A L++  +   
Sbjct: 392 IHHSADIFSNPGKFDPSRF------EVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHH 451

Query: 452 LLRNFELELISPFPEIDWNAMVVGVKGRVMVRYKRRKLSV 486
           L   +   ++     I +    +   G  +V  ++ ++ V
Sbjct: 452 LTTKYSWSIVGASDGIQYGPFALPQNGLPIVLARKPEIEV 467

[Close]

BLAST of Lcy10g007310 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 113.6 bits (283), Expect = 4.4e-25
Identity = 101/425 (23.76%), Postives = 182/425 (42.82%), Query Frame = 0


Query: 32  SKKRAPPTVKGLPLVGGLLR-FLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSA 91
           SK   PP   G P VG   + + + P V  + +  + GSVF  ++L      +  PE +A
Sbjct: 32  SKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCVMISSPE-AA 91

Query: 92  HFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMV 151
            F   ++S L +           G   +F        +  +    A     ++  V  + 
Sbjct: 92  KFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIE 151

Query: 152 KEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF-ADVSALFHDLDNGML 211
             A+D    W +   ++   E++      A   + G++  + L+  D+   ++ L+ G  
Sbjct: 152 SIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFGKD--EVLYREDLKRCYYILEKGYN 211

Query: 212 PISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPT 271
            + V    LP     +  +AR +L+ I A+I++ R+ NGSS ND+L  F+  K       
Sbjct: 212 SMPV---NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMGDK----EEL 271

Query: 272 TDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLM--GKHGDKIDH 331
           TD ++   +I  +FA + T++   +W   YL  +   + AV EEQ  +    + G+ +  
Sbjct: 272 TDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTW 331

Query: 332 DILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFAN 391
               +M    R I+E LR+   L    R +  D      E + ++IPKG  +   P F N
Sbjct: 332 GDTKKMPLTSRVIQETLRVASILSFTFREAVEDV-----EYEGYLIPKGWKVL--PLFRN 391

Query: 392 --RLPHVYNDPDRYDPDRFAPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQ 451
                 ++++P ++DP RF      + A    +++ FG G H C G   A L++  +   
Sbjct: 392 IHHSADIFSNPGKFDPSRF------EVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHH 432

The following BLAST results are available for this feature:

BLAST of Lcy10g007310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAA9	2.5e-238	82.72	Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1	[more]
P93846	1.4e-222	75.83	Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1	[more]
P93596	1.1e-219	80.49	Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP...	[more]
Q1ZXH9	1.8e-103	42.89	Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN...	[more]
Q4PJW3	5.9e-91	39.69	Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1	[more]

BLAST of Lcy10g007310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2S4	1.6e-269	94.85	sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111439144 PE=3 SV...	[more]
A0A6J1J0F8	3.6e-269	95.06	sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1	[more]
A0A6J1I3Z5	4.0e-268	94.43	sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111470769 PE=3 SV=1	[more]
A0A6J1GUN6	5.3e-268	94.65	sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV...	[more]
A0A0A0KYP3	1.5e-267	94.25	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1	[more]

BLAST of Lcy10g007310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932660.1	3.4e-269	94.85	sterol 14-demethylase-like [Cucurbita moschata] >XP_022932661.1 sterol 14-demeth...	[more]
KAG6597695.1	3.4e-269	94.85	Sterol 14-demethylase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022980879.1	7.5e-269	95.06	sterol 14-demethylase-like [Cucurbita maxima]	[more]
XP_023527569.1	9.8e-269	95.06	sterol 14-demethylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023539138.1	9.8e-269	94.85	sterol 14-demethylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy10g007310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11680.1	1.7e-239	82.72	CYTOCHROME P450 51G1	[more]
AT5G45340.1	4.3e-28	24.52	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT5G45340.2	8.0e-27	25.17	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	1.8e-26	22.83	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT4G19230.2	4.4e-25	23.76	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 333..349 score: 46.61 coord: 58..81 score: 35.1 coord: 275..301 score: 37.32 coord: 36..53 score: 24.57 coord: 371..385 score: 35.9 coord: 429..447 score: 43.52 coord: 387..405 score: 27.94 coord: 413..429 score: 34.39
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 284..301 score: 32.57 coord: 420..429 score: 40.95 coord: 338..349 score: 40.69 coord: 429..440 score: 38.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 37..456 e-value: 2.3E-66 score: 224.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 27..485 e-value: 2.9E-122 score: 411.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..481
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 10..481 e-value: 7.6E-35 score: 118.6
None	No IPR available	PANTHER	PTHR24286:SF251	STEROL 14-DEMETHYLASE	coord: 8..479
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 8..479
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 422..431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy10g007310.1	Lcy10g007310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0016126	sterol biosynthetic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen