Lcy10g007160 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g007160
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAuxin-responsive GH3 family protein
LocationChr10: 29830835 .. 29833384 (-)
RNA-Seq ExpressionLcy10g007160
SyntenyLcy10g007160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGATACCTGGAAACAGAATCCCTCCACAAAGAAAAAATTGCACAGTCAAATTGTCAGTTTATAGTTATAGTTATGAGCTGATTGAGAAGAAAATGCAAAAGCTAGCTACCTTCTTGTGCTGTTTCATTCATTGCAAATATTTTTGATGCGATGTTATAAAATTAAAACTCCAGTTTCTGTCCAAATCCCAAACTCCTTCTTTCAAACCCTCTTCTTCTTCTCTCGATCATTCCCCTCCTCCTTTAGAGATGTTGCCAAATTTTGATCCAAACGACAACGAAGCTGGGCTGAAGCTACTGGAGGATCTGACACAAAATGCAAGGCAAATACAAGAGCAAGTCATTCACAAGATTCTCACACAAAACGCCAACACAGAATATCTCAAGACCTTCATCAATGGCCACTCTCCCGACGTCCAACTTTTCAAGAACACTGTCCCTGTGGTCAACTACGAAGACATTAAGCCTTATATCGAGAGAATTGCCAATGGAGAGCCTTCCAACATAATCTCAGCCCAACCCATCACTGAGCTACTCACAAGGTACTCTATTTATTCAACACTCTCTCATTTTCTTTCAACCGTGTGTTTAAAGCCCTGATGTTTCAAAACCAAATGCAGCTCTGGAACTTCTGGTGGCCAGCCAAAGATGATGCCTTCTACAGCCGAGGACTTGGACAGAAAGACATTCTTTTACAACCTCCTCGTCCCTGTGATGAACAAGTACTCAAAATTAAAACACAACAAAGCTTTAAGCTTCTCCCTTTACGAATAAAACAGTGCTTAACTTTATTTTTTTGGTGGTCCAATGTTTCAGGCATGTGGATGGTTTGGAGGAAGGAAAAGGGATGTACCTTTTGTTTATAAAACCAGAGATCAGCACTCCATCTGGGTTGATGGCGAGGCCAGTTCTGACGAGCTATTACAAAAGCAAAAACTTCAGGAATAGGCCTTTCAATAGGTACAATGTGTACACAAGTCCCGATGAGACGATTCTGTGCTCCGACAGTAAGCAGAGCATGTACTGCCAATTGCTTTGTGGGTTGGTACAGAGGGACGAGGTTTTGAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGGGCCATCAAATTCTTAGAAGATCACTGGAAGGAGCTAAGCGATAACATAAGAAACGGTCATGTCAGTGACTGGATCACCGACCCCAGTTGCAGAACTGCTGTTTCTTTAATCCTCGCAAAGCCCAACGCCGCTCTGGCCGATTTGATCCACGCCAAGTGCGGCGAGAAATCTTGGGAAGGCATAATCAAGAAGATTTGGCCTAAAACCAAATACATTGAGGTTATTGTAACTGGCTCCATGGCTCAGTATATTCCCACGCTTGAATTCTACAGCGGTGGGCTTCCTTTAGTTTCGACCATGTACGCTTCCTCTGAGTGCTATTTCGGGATCAATTTCGATCCTTTGAGCAAACCGTCTGATGTTTCTTATACCCTTCTTCCAAACATGGCCTTCTTCGAGTTCTTGCCTGTGGAGAAAAACGGTGGAGAGTTGCAACCCCACCAAAGCCACTGCAATGGAGGCTCTGCTCAACCAATTGACGAATTCGAAACGGTGGATCTCGTCGATGTGGAACTCGGTCACTATTACGAATTAGTGGTCACCACTTTCACAGGTGAAAACCCCCTTTCCCTGTTTTTTTTTTTTTTTCTTTTTCCTTTTTGGATCTGACCCACATCATTTGTTAAGTTCATTAGCGGAGAAAAAGACTATTCAACTAAACCCAGGTTCAAATCAGTCTTCGGTACTAATTTTGTTTTATAATTAATCAAAATCATAACGTTGGTTGGTTTATCTTTTCTTGTGGGTGCGTTCAGGGCTATACAGATACAGAGTTGGCGACATTCTAATGGTAACAGGATTCCACAACAAGGCGCCGCAGTTCCGGTTCGTCCATCGACGGAACGTGGTGTTAAGCATAGACACGGACAAGACGAACGAAGACGACCTGTTGAAGGCAATAACAAAGGCGAAACTGCTGCTGGAGCCGCTGGGTTTTCTGTTGACAGAGTACACTAGCTATGCAGACACGGGCTCGATTCCAGGGCATTACGTGCTGTTTTGGGAGGTGAAGAAGAGAGGGGGCAGTGAGAATTTGGCAGAGCTGGAGTTGGATGTGATGGAAGAATGCTGTTCGAGTGTGGAACAATCTCTGGATTCTGTGTACAGAAGGTGCAGAAACAAGGATAAATCGATTGGGCCATTGGAGATTAGGGTTGTGAAACATGGAGCTTTTGATGCACTCATGGATTTTTGTGTGTCACAAGGCTCTTCTGTGAACCAATACAAGACGCCCAGATGCATTAAATCGGAGGAGGCTATTAAAATCCTGGATTCCAGAGTGGTGGGGAGATTTTTCAGCAGGAAAACGCCGTTTTGGGAGCCCTTCAGAGTGGAGTGCTCGCAATGACCACGACTCTAGTAGTGTGTTTATGTGGATTTTTTTTTTTTTTTTCTTTTTAGTCTTTTTTTCTGACTCATTTTTCTGGGAAATTGTGCCAGAGATGGA

mRNA sequence

GCAGATACCTGGAAACAGAATCCCTCCACAAAGAAAAAATTGCACAGTCAAATTGTCAGTTTATAGTTATAGTTATGAGCTGATTGAGAAGAAAATGCAAAAGCTAGCTACCTTCTTGTGCTGTTTCATTCATTGCAAATATTTTTGATGCGATGTTATAAAATTAAAACTCCAGTTTCTGTCCAAATCCCAAACTCCTTCTTTCAAACCCTCTTCTTCTTCTCTCGATCATTCCCCTCCTCCTTTAGAGATGTTGCCAAATTTTGATCCAAACGACAACGAAGCTGGGCTGAAGCTACTGGAGGATCTGACACAAAATGCAAGGCAAATACAAGAGCAAGTCATTCACAAGATTCTCACACAAAACGCCAACACAGAATATCTCAAGACCTTCATCAATGGCCACTCTCCCGACGTCCAACTTTTCAAGAACACTGTCCCTGTGGTCAACTACGAAGACATTAAGCCTTATATCGAGAGAATTGCCAATGGAGAGCCTTCCAACATAATCTCAGCCCAACCCATCACTGAGCTACTCACAAGCTCTGGAACTTCTGGTGGCCAGCCAAAGATGATGCCTTCTACAGCCGAGGACTTGGACAGAAAGACATTCTTTTACAACCTCCTCGTCCCTGTGATGAACAAGCATGTGGATGGTTTGGAGGAAGGAAAAGGGATGTACCTTTTGTTTATAAAACCAGAGATCAGCACTCCATCTGGGTTGATGGCGAGGCCAGTTCTGACGAGCTATTACAAAAGCAAAAACTTCAGGAATAGGCCTTTCAATAGGTACAATGTGTACACAAGTCCCGATGAGACGATTCTGTGCTCCGACAGTAAGCAGAGCATGTACTGCCAATTGCTTTGTGGGTTGGTACAGAGGGACGAGGTTTTGAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGGGCCATCAAATTCTTAGAAGATCACTGGAAGGAGCTAAGCGATAACATAAGAAACGGTCATGTCAGTGACTGGATCACCGACCCCAGTTGCAGAACTGCTGTTTCTTTAATCCTCGCAAAGCCCAACGCCGCTCTGGCCGATTTGATCCACGCCAAGTGCGGCGAGAAATCTTGGGAAGGCATAATCAAGAAGATTTGGCCTAAAACCAAATACATTGAGGTTATTGTAACTGGCTCCATGGCTCAGTATATTCCCACGCTTGAATTCTACAGCGGTGGGCTTCCTTTAGTTTCGACCATGTACGCTTCCTCTGAGTGCTATTTCGGGATCAATTTCGATCCTTTGAGCAAACCGTCTGATGTTTCTTATACCCTTCTTCCAAACATGGCCTTCTTCGAGTTCTTGCCTGTGGAGAAAAACGGTGGAGAGTTGCAACCCCACCAAAGCCACTGCAATGGAGGCTCTGCTCAACCAATTGACGAATTCGAAACGGTGGATCTCGTCGATGTGGAACTCGGTCACTATTACGAATTAGTGGTCACCACTTTCACAGGGCTATACAGATACAGAGTTGGCGACATTCTAATGGTAACAGGATTCCACAACAAGGCGCCGCAGTTCCGGTTCGTCCATCGACGGAACGTGGTGTTAAGCATAGACACGGACAAGACGAACGAAGACGACCTGTTGAAGGCAATAACAAAGGCGAAACTGCTGCTGGAGCCGCTGGGTTTTCTGTTGACAGAGTACACTAGCTATGCAGACACGGGCTCGATTCCAGGGCATTACGTGCTGTTTTGGGAGGTGAAGAAGAGAGGGGGCAGTGAGAATTTGGCAGAGCTGGAGTTGGATGTGATGGAAGAATGCTGTTCGAGTGTGGAACAATCTCTGGATTCTGTGTACAGAAGGTGCAGAAACAAGGATAAATCGATTGGGCCATTGGAGATTAGGGTTGTGAAACATGGAGCTTTTGATGCACTCATGGATTTTTGTGTGTCACAAGGCTCTTCTGTGAACCAATACAAGACGCCCAGATGCATTAAATCGGAGGAGGCTATTAAAATCCTGGATTCCAGAGTGGTGGGGAGATTTTTCAGCAGGAAAACGCCGTTTTGGGAGCCCTTCAGAGTGGAGTGCTCGCAATGACCACGACTCTAGTAGTGTGTTTATGTGGATTTTTTTTTTTTTTTTCTTTTTAGTCTTTTTTTCTGACTCATTTTTCTGGGAAATTGTGCCAGAGATGGA

Coding sequence (CDS)

ATGTTGCCAAATTTTGATCCAAACGACAACGAAGCTGGGCTGAAGCTACTGGAGGATCTGACACAAAATGCAAGGCAAATACAAGAGCAAGTCATTCACAAGATTCTCACACAAAACGCCAACACAGAATATCTCAAGACCTTCATCAATGGCCACTCTCCCGACGTCCAACTTTTCAAGAACACTGTCCCTGTGGTCAACTACGAAGACATTAAGCCTTATATCGAGAGAATTGCCAATGGAGAGCCTTCCAACATAATCTCAGCCCAACCCATCACTGAGCTACTCACAAGCTCTGGAACTTCTGGTGGCCAGCCAAAGATGATGCCTTCTACAGCCGAGGACTTGGACAGAAAGACATTCTTTTACAACCTCCTCGTCCCTGTGATGAACAAGCATGTGGATGGTTTGGAGGAAGGAAAAGGGATGTACCTTTTGTTTATAAAACCAGAGATCAGCACTCCATCTGGGTTGATGGCGAGGCCAGTTCTGACGAGCTATTACAAAAGCAAAAACTTCAGGAATAGGCCTTTCAATAGGTACAATGTGTACACAAGTCCCGATGAGACGATTCTGTGCTCCGACAGTAAGCAGAGCATGTACTGCCAATTGCTTTGTGGGTTGGTACAGAGGGACGAGGTTTTGAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGGGCCATCAAATTCTTAGAAGATCACTGGAAGGAGCTAAGCGATAACATAAGAAACGGTCATGTCAGTGACTGGATCACCGACCCCAGTTGCAGAACTGCTGTTTCTTTAATCCTCGCAAAGCCCAACGCCGCTCTGGCCGATTTGATCCACGCCAAGTGCGGCGAGAAATCTTGGGAAGGCATAATCAAGAAGATTTGGCCTAAAACCAAATACATTGAGGTTATTGTAACTGGCTCCATGGCTCAGTATATTCCCACGCTTGAATTCTACAGCGGTGGGCTTCCTTTAGTTTCGACCATGTACGCTTCCTCTGAGTGCTATTTCGGGATCAATTTCGATCCTTTGAGCAAACCGTCTGATGTTTCTTATACCCTTCTTCCAAACATGGCCTTCTTCGAGTTCTTGCCTGTGGAGAAAAACGGTGGAGAGTTGCAACCCCACCAAAGCCACTGCAATGGAGGCTCTGCTCAACCAATTGACGAATTCGAAACGGTGGATCTCGTCGATGTGGAACTCGGTCACTATTACGAATTAGTGGTCACCACTTTCACAGGGCTATACAGATACAGAGTTGGCGACATTCTAATGGTAACAGGATTCCACAACAAGGCGCCGCAGTTCCGGTTCGTCCATCGACGGAACGTGGTGTTAAGCATAGACACGGACAAGACGAACGAAGACGACCTGTTGAAGGCAATAACAAAGGCGAAACTGCTGCTGGAGCCGCTGGGTTTTCTGTTGACAGAGTACACTAGCTATGCAGACACGGGCTCGATTCCAGGGCATTACGTGCTGTTTTGGGAGGTGAAGAAGAGAGGGGGCAGTGAGAATTTGGCAGAGCTGGAGTTGGATGTGATGGAAGAATGCTGTTCGAGTGTGGAACAATCTCTGGATTCTGTGTACAGAAGGTGCAGAAACAAGGATAAATCGATTGGGCCATTGGAGATTAGGGTTGTGAAACATGGAGCTTTTGATGCACTCATGGATTTTTGTGTGTCACAAGGCTCTTCTGTGAACCAATACAAGACGCCCAGATGCATTAAATCGGAGGAGGCTATTAAAATCCTGGATTCCAGAGTGGTGGGGAGATTTTTCAGCAGGAAAACGCCGTTTTGGGAGCCCTTCAGAGTGGAGTGCTCGCAATGA

Protein sequence

MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHSPDVQLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSYADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWEPFRVECSQ
Homology
BLAST of Lcy10g007160 vs. ExPASy Swiss-Prot
Match: Q9FZ87 (Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=GH3.17 PE=1 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 6.8e-262
Identity = 440/609 (72.25%), Postives = 516/609 (84.73%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHS-PDVQLF 60
           M+P++DPND EAGLKLLEDLT NA  IQ+QV+H+IL+QN+ T+YL+ F++G +  + Q F
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN VPVVNY+D+KP+I+RIA+GE S+I+SAQPITELLTSSGTS G+PK+MPSTAE+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFY++LVP+MNK+VDGL+EGKGMYLLFIKPEI TPSGLMARPVLTSYYKS++FRNRPFN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           +YNVYTSPD+TILC DSKQSMYCQLLCGLVQR  VLRVGAVFASAFLRA+KFLEDH+KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
             +IR G V+ WITD SCR +V  IL  PN  LAD I ++C EKSWEGI+++IWPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN +PL  P+DVSYTLLPNMA+F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 361 EFLPVEKNGGELQPHQSHCN-GGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRV 420
           EFLPV+    E     +H N       + E   V+LV+VE+G YYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKL--LLEPLGFLLTE 480
           GDIL VTGFHNKAPQFRFV RRNVVLSIDTDKT+E+DLL A+T+AKL  L  P   LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 481 YTSYADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDK 540
           YTSYADT SIPGHYVLFWE+K R  S +  +L+   ME+CCS VE  LD VYRRCRN+DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPR-HSNDPPKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKT 600
           SIGPLEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSRV+GRFFS++ 
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

Query: 601 PFWEPFRVE 606
           P WEP  ++
Sbjct: 601 PQWEPLGLD 608

BLAST of Lcy10g007160 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 2.3e-201
Identity = 353/593 (59.53%), Postives = 447/593 (75.38%), Query Frame = 0

Query: 10  NEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTF-INGHSPDVQLFKNTVPVVNY 69
           N+  L+L+E+LT NA Q+Q QV+ +ILT+NA+ EYL+   +NG + D + FKN +PV+ Y
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRT-DRETFKNIMPVITY 80

Query: 70  EDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S I+S++PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           VM++ V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  YTSP+
Sbjct: 141 VMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHV 249
           ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE HW EL  +IR G +
Sbjct: 201 ETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTL 260

Query: 250 SDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITDPS R AV+ IL KP+  LAD +  +C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNG 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KPS+VSYTL+P+MA+FEFLPV +N 
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 380

Query: 370 GELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFH 429
           G      S     +    ++ E VDLVDV+LG  YELVVTT+ GL RYRVGD+L VTGF 
Sbjct: 381 GVT---NSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFK 440

Query: 430 NKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSYADTGSIPG 489
           NKAPQF F+ R+NVVLSID+DKT+E +L  A+  A   L P    L+EYTSYADT SIPG
Sbjct: 441 NKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 500

Query: 490 HYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKH 549
           HYVLFWE+   G +     +   V E+CC +VE+S ++VYR+ R  DKSIGPLEI++V+ 
Sbjct: 501 HYVLFWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEP 560

Query: 550 GAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWEP 602
           G FD LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  +FS K P W P
Sbjct: 561 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of Lcy10g007160 vs. ExPASy Swiss-Prot
Match: P0C0M3 (Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.11 PE=2 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 2.5e-200
Identity = 344/585 (58.80%), Postives = 442/585 (75.56%), Query Frame = 0

Query: 14  LKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHSPDVQLFKNTVPVVNYEDIKP 73
           L+ LE LT NA++ QE ++ KIL +N  TEYL  F+NG S ++  FK  VPVV Y+ + P
Sbjct: 14  LEELEMLTVNAKEAQELILTKILERNQATEYLSKFMNG-STNISAFKRHVPVVTYDKVHP 73

Query: 74  YIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVMNKH 133
           YI RIA GE S+I+  + I ELL SSGTS G+P++MPS  +DLDR+T+ Y+L++P+MNK+
Sbjct: 74  YILRIATGEESSILCGEYILELLRSSGTSRGEPRLMPSILKDLDRRTYLYSLIMPIMNKY 133

Query: 134 VDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILC 193
           + GL EGK MYLLF+K E  T SG+  R VLTSYYKS +F +R  + YN YTSPDE ILC
Sbjct: 134 ISGLGEGKAMYLLFVKAETLTDSGIPVRSVLTSYYKSPHFLHRKHDLYNNYTSPDEVILC 193

Query: 194 SDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHVSDWIT 253
            DS+QSMYCQLLCGLV+R  VLR+GAVFASAFLR+I FLE HW++L ++IR G ++  IT
Sbjct: 194 PDSQQSMYCQLLCGLVERQHVLRIGAVFASAFLRSISFLEQHWRDLVNDIRIGQLNSSIT 253

Query: 254 DPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPT 313
            P+CR A+   LA PN  LAD + A C   SW+GI+ ++WP  KYIE ++TG+MAQYIP 
Sbjct: 254 SPACRLAMLNFLALPNPELADQVEAICSCGSWKGILGRLWPNVKYIEAVLTGTMAQYIPM 313

Query: 314 LEFYSGG-LPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNGGELQ 373
           LEFY GG +P V TMYASSE YFG+N  PL  P+DVSYT+LPNMA+FEF+P+E +G  L 
Sbjct: 314 LEFYGGGAIPFVCTMYASSESYFGVNLSPLCSPADVSYTILPNMAYFEFIPLE-DGLRLT 373

Query: 374 PHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFHNKAP 433
            H+        + I+  + V LVDV++G YYELVVTTF+GLYRYRVGD+L VTGF+N+AP
Sbjct: 374 DHE--------EVIENDKLVSLVDVKVGCYYELVVTTFSGLYRYRVGDVLQVTGFYNRAP 433

Query: 434 QFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSYADTGSIPGHYVL 493
           QF+F+ RRNV+LSID+DKTNE+DL  ++T AK +LE   +LL EYTSY D  ++PGHYVL
Sbjct: 434 QFKFICRRNVILSIDSDKTNEEDLHNSVTTAKKILENQNYLLLEYTSYTDISTVPGHYVL 493

Query: 494 FWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKHGAFD 553
           FWE+K     E  A L+  ++E CC++VE+SLD VYRRCR  D+SIGPLEIR+V+ GAFD
Sbjct: 494 FWEIKST-HDERPAPLDAQLLESCCAAVEESLDYVYRRCRAHDRSIGPLEIRLVEAGAFD 553

Query: 554 ALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTP 598
           ALMD  VS GSS+NQYKTPRCI+S  A+K+L+S+V+  FFS + P
Sbjct: 554 ALMDLLVSHGSSINQYKTPRCIESSLALKLLNSKVIACFFSPQDP 587

BLAST of Lcy10g007160 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.6e-199
Identity = 345/592 (58.28%), Postives = 441/592 (74.49%), Query Frame = 0

Query: 10  NEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHSPDVQLFKNTVPVVNYE 69
           N+  L+ +ED+T NA  +Q +V+ +IL++NA+ EYLK        D + FK+ +PVV YE
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYE 80

Query: 70  DIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPV 129
           DI+P I RIANG+ S ++ + PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+PV
Sbjct: 81  DIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPV 140

Query: 130 MNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDE 189
           M++ V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  YTSP++
Sbjct: 141 MDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQ 200

Query: 190 TILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHVS 249
           TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE HW EL+ +IR G +S
Sbjct: 201 TILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLS 260

Query: 250 DWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMAQ 309
             ITD S R AV  IL KP+  LAD + ++C + SW+GII ++WP TKY++VIVTG+M+Q
Sbjct: 261 SEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQ 320

Query: 310 YIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNGG 369
           YIPTL++YS GLPLV TMYASSECYFG+N  PL KPS+VSYTL+PNMA+FEFLPV +N G
Sbjct: 321 YIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSG 380

Query: 370 ELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFHN 429
                 S     +    ++ E VDLVDV+LG  YELVVTT+ GLYRYRVGD+L V GF N
Sbjct: 381 VT---SSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKN 440

Query: 430 KAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSYADTGSIPGH 489
            APQF F+ R+NVVLSID+DKT+E +L  A+  A   L P    L+EYTSYADT SIPGH
Sbjct: 441 NAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 500

Query: 490 YVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKHG 549
           YVLFWE+   G +     +   V E+CC ++E+SL+SVYR+ R  DKSIGPLEI++V+ G
Sbjct: 501 YVLFWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 560

Query: 550 AFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWEP 602
            FD LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  +FS K P W P
Sbjct: 561 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of Lcy10g007160 vs. ExPASy Swiss-Prot
Match: Q8GZ29 (Indole-3-acetic acid-amido synthetase GH3.15 OS=Arabidopsis thaliana OX=3702 GN=GH3.15 PE=1 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 4.1e-198
Identity = 343/600 (57.17%), Postives = 436/600 (72.67%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHSPDVQLFK 60
           MLP FDP + +A L LLEDLT N +QIQ+ V+  IL++NA TEYL+ F+NG   D Q FK
Sbjct: 1   MLPKFDPTNQKACLSLLEDLTTNVKQIQDSVLEAILSRNAQTEYLRGFLNG-QVDKQNFK 60

Query: 61  NTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
             VPVV YEDI+ YI+RIANGEPS++I  +PI+ LLTSSGTSGG PK++P T EDL+++ 
Sbjct: 61  KNVPVVTYEDIRSYIDRIANGEPSDLICDRPISVLLTSSGTSGGVPKLIPLTTEDLEQRI 120

Query: 121 FFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNR 180
            F +L  P++ KH+DGL EGK +   F+  E  T +GLM R ++TS+ KS    N  F  
Sbjct: 121 SFSSLYAPLLYKHIDGLSEGKSLIFYFVTRESKTANGLMVRTMVTSFLKSIKQTN-SFLW 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELS 240
            ++  SP     C+D+ QSMYCQLLCGL++RD V R+GA FAS+FL+ IKFLEDHW EL 
Sbjct: 181 DSLQVSPHAITTCADTTQSMYCQLLCGLLERDNVARLGAPFASSFLKVIKFLEDHWPELC 240

Query: 241 DNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIE 300
            NIR G +SDWITD +C + +   L  PN  LA LI  +C + SWE I+K++WPK K IE
Sbjct: 241 SNIRTGRLSDWITDATCTSGIGKFLTAPNPELASLIEQECSKTSWEAILKRLWPKAKCIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFE 360
            I+TG+MAQYIP LEFYSGGLPL S+ Y SSEC+ G+NF+PL KPSDVSYT++P M +FE
Sbjct: 301 SIITGTMAQYIPLLEFYSGGLPLTSSFYGSSECFMGVNFNPLCKPSDVSYTIIPCMGYFE 360

Query: 361 FLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGD 420
           FL VEK+      HQ   +  + +P+     VDLVDV++GH YE VVTTF+GLYRYRVGD
Sbjct: 361 FLEVEKD------HQEAGHDPTEKPV----VVDLVDVKIGHDYEPVVTTFSGLYRYRVGD 420

Query: 421 ILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSY 480
           +L  TGF+N AP F FV R+ VVLSID DKT EDDLLKA+T AKLLLEP   +L ++TS 
Sbjct: 421 VLRATGFYNNAPHFCFVGRQKVVLSIDMDKTYEDDLLKAVTNAKLLLEPHDLMLMDFTSR 480

Query: 481 ADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGP 540
            D+ S PGHYV++WE+  +       E   DVMEECC +VE+SLD+VYR+ R  DK+IGP
Sbjct: 481 VDSSSFPGHYVIYWELGSKVKDAKF-EPNRDVMEECCFTVEESLDAVYRKGRKNDKNIGP 540

Query: 541 LEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWE 600
           LEI+VVK GAFD LM+F +S+GSSV+QYKTPR + +EEA+KIL++ V+  F SRK P WE
Sbjct: 541 LEIKVVKPGAFDELMNFFLSRGSSVSQYKTPRSVTNEEALKILEANVISEFLSRKIPSWE 587

BLAST of Lcy10g007160 vs. ExPASy TrEMBL
Match: A0A6J1ISD7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC111480114 PE=3 SV=1)

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 560/609 (91.95%), Postives = 580/609 (95.24%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-HSPDVQLF 60
           ML +FDPNDNE GLKLLEDLTQNARQIQEQVI KILTQNANTEYLK+FING HSPDV LF
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPVMNK+VDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
           SDNIR+G+VSDWITDP+CR +VSL+L KPNAALADLI + CGEK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKPSDVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVG 420
           EFLPVEKN GEL    SHCNGGS  P DEFETVDLVDV+LGHYYELVVTTFTGLYRYRVG
Sbjct: 361 EFLPVEKNNGEL----SHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTS 480
           DILMVTGFHN APQFRFVHRRNVVLSIDTDKTNEDDLL AITKAKLLLEPLG LLTEYTS
Sbjct: 421 DILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIG 540
           YADTGSIPGHYVLFWE+K RGG E+L ELE  +MEECCSSVEQSLDSVYRRCR+KDKSIG
Sbjct: 481 YADTGSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIG 540

Query: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFW 600
           PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR VGRFFSRKTPFW
Sbjct: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRAVGRFFSRKTPFW 600

Query: 601 EPFRVECSQ 609
           EPFR + SQ
Sbjct: 601 EPFRAQYSQ 605

BLAST of Lcy10g007160 vs. ExPASy TrEMBL
Match: A0A6J1GWE7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LOC111457496 PE=3 SV=1)

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 556/609 (91.30%), Postives = 579/609 (95.07%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-HSPDVQLF 60
           MLP+FDPNDNE GLKLLEDLTQNARQIQEQVI KILTQNANTEYLK+FING HSPDV LF
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KNTVPVVNYEDIKPYIERIANGEPSNIISAQPI ELLTSSGTSGG PKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPIIELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPVMNK+VDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGLVQR++VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQREDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
           SDNIR+G+VSDWITDP+CR +VSL+L KPNAALADLI A CGEK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKPSDVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVG 420
           EFLPVEKN GEL    SHCNGGS  P DEFETVDLVDV+LGHYYELVVTTFTGLYRYRVG
Sbjct: 361 EFLPVEKNNGEL----SHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTS 480
           DILMVTGFHN APQFRFVHRRNVVLSIDTDKTNEDDLL AITKAKLLLEPLG LLTEYTS
Sbjct: 421 DILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIG 540
           YADTGSIPGHYVLFWE+K RGG E+L ELE  +MEECCSSVE+SLDSVYRRCR+KDKSIG
Sbjct: 481 YADTGSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEKSLDSVYRRCRSKDKSIG 540

Query: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFW 600
           PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRFFSRKTPFW
Sbjct: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFW 600

Query: 601 EPFRVECSQ 609
           EPFR + SQ
Sbjct: 601 EPFRAQYSQ 605

BLAST of Lcy10g007160 vs. ExPASy TrEMBL
Match: A0A6J1DHN6 (indole-3-acetic acid-amido synthetase GH3.17-like OS=Momordica charantia OX=3673 GN=LOC111020995 PE=3 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 541/621 (87.12%), Postives = 572/621 (92.11%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-----HSPD 60
           MLPNFDPNDNE+GLKLLEDLTQNARQIQEQVI KIL+QNANTEYLK FIN       SPD
Sbjct: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60

Query: 61  VQLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           ++LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNKHV GLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDH 240
           RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240

Query: 241 WKELSDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPK 300
           WKE+S NIR+G VS+WITDPSCR++VSLIL KPN  LADLI A CGEKSWEGIIKKIW K
Sbjct: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360

Query: 361 MAFFEFLPVEKN-GGELQPHQSHCNGGSAQ----PIDEFETVDLVDVELGHYYELVVTTF 420
           MAFFEFLPVE+N GGEL     H NGG+ +     + + ETVDLVDVELG YYELVVTTF
Sbjct: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420

Query: 421 TGLYRYRVGDILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPL 480
           TGLYRYRVGDIL VTGFHN APQFRF+HRRNVVLSIDTDKTNEDDLLKAIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480

Query: 481 GFLLTEYTSYADTGSIPGHYVLFWEVKKR-GGSENLA--ELELDVMEECCSSVEQSLDSV 540
           G LLTEY+SYADT SIPGHYVLFWE+KKR GG EN A   ++L VMEECCSS+E+SLDSV
Sbjct: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540

Query: 541 YRRCRNKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRV 600
           YRRCR+KDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAI+ILDSRV
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600

Query: 601 VGRFFSRKTPFWEPFRVECSQ 609
           VGRFFSRKTPFWEPFRV+CS+
Sbjct: 601 VGRFFSRKTPFWEPFRVDCSK 621

BLAST of Lcy10g007160 vs. ExPASy TrEMBL
Match: A0A5A7TMG2 (Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002300 PE=3 SV=1)

HSP 1 Score: 1074.3 bits (2777), Expect = 2.3e-310
Identity = 529/615 (86.02%), Postives = 571/615 (92.85%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFIN----GHSPDV 60
           MLPNFDPN+NE+GLKLLEDLT NA+QIQEQVI KILTQN+NTEYLK+F++     HS D+
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 61  QLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDL 120
           Q FK++VPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGGQPKMMPSTAEDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 121 DRKTFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNR 180
           DRKTFFYNLLVPV+NK+VDGLEEGKGMYLLFIKPE+STPSGLMARPVLTSYYKSKNFRNR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 181 PFNRYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHW 240
           PFN+YNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLED+W
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 241 KELSDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKT 300
           KELSDNIR G +S WI+DP+CR +VSLIL K N  LADLI   CGEKSWEGIIKK+WPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNM 360
           KYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINF+PLSKPSDVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 361 AFFEFLPVEKNGGELQPHQSHCNGGSAQPI-DEFETVDLVDVELGHYYELVVTTFTGLYR 420
           AFFEFLPVEKN GEL    +HCNG +   + DEF+TVDLVDV+LG YYELVVTTFTGLYR
Sbjct: 361 AFFEFLPVEKNDGEL----THCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYR 420

Query: 421 YRVGDILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLT 480
           YRVGDIL VTGFHNKAPQF+F+HRRNVVLSIDTDKTNEDDLL AITKAKLL+EPLG LLT
Sbjct: 421 YRVGDILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLT 480

Query: 481 EYTSYADTGSIPGHYVLFWEVKKRGGS--ENLAEL-ELDVMEECCSSVEQSLDSVYRRCR 540
           EYTSYADT SIPGHYVLFWE+K+R GS  EN  EL E+D+MEECCSS+E+SLDSVYRRCR
Sbjct: 481 EYTSYADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCR 540

Query: 541 NKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFF 600
           +KDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV R+F
Sbjct: 541 SKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYF 600

Query: 601 SRKTPFWEPFRVECS 608
           S+KTPFWEPFR + S
Sbjct: 601 SKKTPFWEPFRGQYS 611

BLAST of Lcy10g007160 vs. ExPASy TrEMBL
Match: A0A1S3BYQ7 (indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC103494645 PE=3 SV=1)

HSP 1 Score: 1074.3 bits (2777), Expect = 2.3e-310
Identity = 529/615 (86.02%), Postives = 571/615 (92.85%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFIN----GHSPDV 60
           MLPNFDPN+NE+GLKLLEDLT NA+QIQEQVI KILTQN+NTEYLK+F++     HS D+
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 61  QLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDL 120
           Q FK++VPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGGQPKMMPSTAEDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 121 DRKTFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNR 180
           DRKTFFYNLLVPV+NK+VDGLEEGKGMYLLFIKPE+STPSGLMARPVLTSYYKSKNFRNR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 181 PFNRYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHW 240
           PFN+YNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLED+W
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 241 KELSDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKT 300
           KELSDNIR G +S WI+DP+CR +VSLIL K N  LADLI   CGEKSWEGIIKK+WPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNM 360
           KYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINF+PLSKPSDVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 361 AFFEFLPVEKNGGELQPHQSHCNGGSAQPI-DEFETVDLVDVELGHYYELVVTTFTGLYR 420
           AFFEFLPVEKN GEL    +HCNG +   + DEF+TVDLVDV+LG YYELVVTTFTGLYR
Sbjct: 361 AFFEFLPVEKNDGEL----THCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYR 420

Query: 421 YRVGDILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLT 480
           YRVGDIL VTGFHNKAPQF+F+HRRNVVLSIDTDKTNEDDLL AITKAKLL+EPLG LLT
Sbjct: 421 YRVGDILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLT 480

Query: 481 EYTSYADTGSIPGHYVLFWEVKKRGGS--ENLAEL-ELDVMEECCSSVEQSLDSVYRRCR 540
           EYTSYADT SIPGHYVLFWE+K+R GS  EN  EL E+D+MEECCSS+E+SLDSVYRRCR
Sbjct: 481 EYTSYADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCR 540

Query: 541 NKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFF 600
           +KDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV R+F
Sbjct: 541 SKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYF 600

Query: 601 SRKTPFWEPFRVECS 608
           S+KTPFWEPFR + S
Sbjct: 601 SKKTPFWEPFRGQYS 611

BLAST of Lcy10g007160 vs. NCBI nr
Match: XP_022980842.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 560/609 (91.95%), Postives = 580/609 (95.24%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-HSPDVQLF 60
           ML +FDPNDNE GLKLLEDLTQNARQIQEQVI KILTQNANTEYLK+FING HSPDV LF
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPVMNK+VDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
           SDNIR+G+VSDWITDP+CR +VSL+L KPNAALADLI + CGEK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKPSDVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVG 420
           EFLPVEKN GEL    SHCNGGS  P DEFETVDLVDV+LGHYYELVVTTFTGLYRYRVG
Sbjct: 361 EFLPVEKNNGEL----SHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTS 480
           DILMVTGFHN APQFRFVHRRNVVLSIDTDKTNEDDLL AITKAKLLLEPLG LLTEYTS
Sbjct: 421 DILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIG 540
           YADTGSIPGHYVLFWE+K RGG E+L ELE  +MEECCSSVEQSLDSVYRRCR+KDKSIG
Sbjct: 481 YADTGSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIG 540

Query: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFW 600
           PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR VGRFFSRKTPFW
Sbjct: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRAVGRFFSRKTPFW 600

Query: 601 EPFRVECSQ 609
           EPFR + SQ
Sbjct: 601 EPFRAQYSQ 605

BLAST of Lcy10g007160 vs. NCBI nr
Match: KAG6581972.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018391.1 Indole-3-acetic acid-amido synthetase GH3.17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 559/609 (91.79%), Postives = 580/609 (95.24%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-HSPDVQLF 60
           MLP+FDPNDNE GLKLLEDLTQNARQIQEQVI KILTQNANTEYLK+FING HSPDV LF
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGG PKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPVMNK+VDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGLVQRD+VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
           SDNIR+G+VSDWITDP+CR +VSL+L KPNAALADLI A CGEK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKPSDVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVG 420
           EFLPVEKN GEL    SHCNGGS  P DEFETVDLVDV+LGHYYELVVTTFTGLYRYRVG
Sbjct: 361 EFLPVEKNNGEL----SHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTS 480
           DILMVTGFHN APQFRFVHRRNVVLSIDTDKTNEDDLL AITKAKLLLEPLG LLTEYTS
Sbjct: 421 DILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIG 540
           YADTGSIPGHYVLFWE+K RGG E+L ELE  +MEECCSSVEQSLDSVYRRCR+KDKSIG
Sbjct: 481 YADTGSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIG 540

Query: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFW 600
           PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRFFSRKTPFW
Sbjct: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFW 600

Query: 601 EPFRVECSQ 609
           EPFR + SQ
Sbjct: 601 EPFRAQYSQ 605

BLAST of Lcy10g007160 vs. NCBI nr
Match: XP_023528388.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 558/609 (91.63%), Postives = 580/609 (95.24%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-HSPDVQLF 60
           MLP+FDPND+E GLKLLEDLTQNARQIQEQVI KILTQNANTEYLK+FING HSPDV LF
Sbjct: 1   MLPSFDPNDDEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGG PKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPVMNK+VDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGLVQRD+VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
           SDNIR+G+VSDWITDP+CR +VSL+L KPNAALADLI A CGEK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKPSDVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVG 420
           EFLPVEKN GEL    SHCNGGS  P DEFETVDLVDV+LGHYYELVVTTFTGLYRYRVG
Sbjct: 361 EFLPVEKNNGEL----SHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTS 480
           DILMVTGFHN APQFRFVHRRNVVLSIDTDKTNEDDLL AITKAKLLLEPLG LLTEYTS
Sbjct: 421 DILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIG 540
           YADTGSIPGHYVLFWE+K RGG E+L ELE  +MEECCSSVEQSLDSVYRRCR+KDKSIG
Sbjct: 481 YADTGSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIG 540

Query: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFW 600
           PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRFFSRKTPFW
Sbjct: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFW 600

Query: 601 EPFRVECSQ 609
           EPFR + SQ
Sbjct: 601 EPFRAQYSQ 605

BLAST of Lcy10g007160 vs. NCBI nr
Match: XP_022955484.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita moschata])

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 556/609 (91.30%), Postives = 579/609 (95.07%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-HSPDVQLF 60
           MLP+FDPNDNE GLKLLEDLTQNARQIQEQVI KILTQNANTEYLK+FING HSPDV LF
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KNTVPVVNYEDIKPYIERIANGEPSNIISAQPI ELLTSSGTSGG PKMMPSTAEDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPIIELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFYNLLVPVMNK+VDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           RYNVYTSPDETILC+DS+QSMYCQLLCGLVQR++VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQREDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
           SDNIR+G+VSDWITDP+CR +VSL+L KPNAALADLI A CGEK WEGIIKKIWPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKPSDVSYTLLPNMAFF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 361 EFLPVEKNGGELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVG 420
           EFLPVEKN GEL    SHCNGGS  P DEFETVDLVDV+LGHYYELVVTTFTGLYRYRVG
Sbjct: 361 EFLPVEKNNGEL----SHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTS 480
           DILMVTGFHN APQFRFVHRRNVVLSIDTDKTNEDDLL AITKAKLLLEPLG LLTEYTS
Sbjct: 421 DILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIG 540
           YADTGSIPGHYVLFWE+K RGG E+L ELE  +MEECCSSVE+SLDSVYRRCR+KDKSIG
Sbjct: 481 YADTGSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEKSLDSVYRRCRSKDKSIG 540

Query: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFW 600
           PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRFFSRKTPFW
Sbjct: 541 PLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFW 600

Query: 601 EPFRVECSQ 609
           EPFR + SQ
Sbjct: 601 EPFRAQYSQ 605

BLAST of Lcy10g007160 vs. NCBI nr
Match: XP_022153513.1 (indole-3-acetic acid-amido synthetase GH3.17-like [Momordica charantia])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 541/621 (87.12%), Postives = 572/621 (92.11%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFING-----HSPD 60
           MLPNFDPNDNE+GLKLLEDLTQNARQIQEQVI KIL+QNANTEYLK FIN       SPD
Sbjct: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60

Query: 61  VQLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           ++LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNKHV GLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDH 240
           RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240

Query: 241 WKELSDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPK 300
           WKE+S NIR+G VS+WITDPSCR++VSLIL KPN  LADLI A CGEKSWEGIIKKIW K
Sbjct: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360

Query: 361 MAFFEFLPVEKN-GGELQPHQSHCNGGSAQ----PIDEFETVDLVDVELGHYYELVVTTF 420
           MAFFEFLPVE+N GGEL     H NGG+ +     + + ETVDLVDVELG YYELVVTTF
Sbjct: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420

Query: 421 TGLYRYRVGDILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPL 480
           TGLYRYRVGDIL VTGFHN APQFRF+HRRNVVLSIDTDKTNEDDLLKAIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480

Query: 481 GFLLTEYTSYADTGSIPGHYVLFWEVKKR-GGSENLA--ELELDVMEECCSSVEQSLDSV 540
           G LLTEY+SYADT SIPGHYVLFWE+KKR GG EN A   ++L VMEECCSS+E+SLDSV
Sbjct: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540

Query: 541 YRRCRNKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRV 600
           YRRCR+KDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAI+ILDSRV
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600

Query: 601 VGRFFSRKTPFWEPFRVECSQ 609
           VGRFFSRKTPFWEPFRV+CS+
Sbjct: 601 VGRFFSRKTPFWEPFRVDCSK 621

BLAST of Lcy10g007160 vs. TAIR 10
Match: AT1G28130.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 904.4 bits (2336), Expect = 4.8e-263
Identity = 440/609 (72.25%), Postives = 516/609 (84.73%), Query Frame = 0

Query: 1   MLPNFDPNDNEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHS-PDVQLF 60
           M+P++DPND EAGLKLLEDLT NA  IQ+QV+H+IL+QN+ T+YL+ F++G +  + Q F
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN VPVVNY+D+KP+I+RIA+GE S+I+SAQPITELLTSSGTS G+PK+MPSTAE+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 121 TFFYNLLVPVMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180
           TFFY++LVP+MNK+VDGL+EGKGMYLLFIKPEI TPSGLMARPVLTSYYKS++FRNRPFN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEL 240
           +YNVYTSPD+TILC DSKQSMYCQLLCGLVQR  VLRVGAVFASAFLRA+KFLEDH+KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 241 SDNIRNGHVSDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYI 300
             +IR G V+ WITD SCR +V  IL  PN  LAD I ++C EKSWEGI+++IWPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN +PL  P+DVSYTLLPNMA+F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 361 EFLPVEKNGGELQPHQSHCN-GGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRV 420
           EFLPV+    E     +H N       + E   V+LV+VE+G YYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKL--LLEPLGFLLTE 480
           GDIL VTGFHNKAPQFRFV RRNVVLSIDTDKT+E+DLL A+T+AKL  L  P   LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 481 YTSYADTGSIPGHYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDK 540
           YTSYADT SIPGHYVLFWE+K R  S +  +L+   ME+CCS VE  LD VYRRCRN+DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPR-HSNDPPKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKT 600
           SIGPLEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSRV+GRFFS++ 
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

Query: 601 PFWEPFRVE 606
           P WEP  ++
Sbjct: 601 PQWEPLGLD 608

BLAST of Lcy10g007160 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 703.4 bits (1814), Expect = 1.6e-202
Identity = 353/593 (59.53%), Postives = 447/593 (75.38%), Query Frame = 0

Query: 10  NEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTF-INGHSPDVQLFKNTVPVVNY 69
           N+  L+L+E+LT NA Q+Q QV+ +ILT+NA+ EYL+   +NG + D + FKN +PV+ Y
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRT-DRETFKNIMPVITY 80

Query: 70  EDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S I+S++PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VMNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           VM++ V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  YTSP+
Sbjct: 141 VMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHV 249
           ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE HW EL  +IR G +
Sbjct: 201 ETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTL 260

Query: 250 SDWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITDPS R AV+ IL KP+  LAD +  +C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNG 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KPS+VSYTL+P+MA+FEFLPV +N 
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 380

Query: 370 GELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFH 429
           G      S     +    ++ E VDLVDV+LG  YELVVTT+ GL RYRVGD+L VTGF 
Sbjct: 381 GVT---NSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFK 440

Query: 430 NKAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSYADTGSIPG 489
           NKAPQF F+ R+NVVLSID+DKT+E +L  A+  A   L P    L+EYTSYADT SIPG
Sbjct: 441 NKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 500

Query: 490 HYVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKH 549
           HYVLFWE+   G +     +   V E+CC +VE+S ++VYR+ R  DKSIGPLEI++V+ 
Sbjct: 501 HYVLFWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEP 560

Query: 550 GAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWEP 602
           G FD LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  +FS K P W P
Sbjct: 561 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of Lcy10g007160 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 697.2 bits (1798), Expect = 1.2e-200
Identity = 345/592 (58.28%), Postives = 441/592 (74.49%), Query Frame = 0

Query: 10  NEAGLKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHSPDVQLFKNTVPVVNYE 69
           N+  L+ +ED+T NA  +Q +V+ +IL++NA+ EYLK        D + FK+ +PVV YE
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYE 80

Query: 70  DIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPV 129
           DI+P I RIANG+ S ++ + PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+PV
Sbjct: 81  DIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPV 140

Query: 130 MNKHVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDE 189
           M++ V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  YTSP++
Sbjct: 141 MDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQ 200

Query: 190 TILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHVS 249
           TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE HW EL+ +IR G +S
Sbjct: 201 TILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLS 260

Query: 250 DWITDPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMAQ 309
             ITD S R AV  IL KP+  LAD + ++C + SW+GII ++WP TKY++VIVTG+M+Q
Sbjct: 261 SEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQ 320

Query: 310 YIPTLEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNGG 369
           YIPTL++YS GLPLV TMYASSECYFG+N  PL KPS+VSYTL+PNMA+FEFLPV +N G
Sbjct: 321 YIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSG 380

Query: 370 ELQPHQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFHN 429
                 S     +    ++ E VDLVDV+LG  YELVVTT+ GLYRYRVGD+L V GF N
Sbjct: 381 VT---SSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKN 440

Query: 430 KAPQFRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSYADTGSIPGH 489
            APQF F+ R+NVVLSID+DKT+E +L  A+  A   L P    L+EYTSYADT SIPGH
Sbjct: 441 NAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 500

Query: 490 YVLFWEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKHG 549
           YVLFWE+   G +     +   V E+CC ++E+SL+SVYR+ R  DKSIGPLEI++V+ G
Sbjct: 501 YVLFWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 560

Query: 550 AFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWEP 602
            FD LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  +FS K P W P
Sbjct: 561 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of Lcy10g007160 vs. TAIR 10
Match: AT1G28130.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 695.7 bits (1794), Expect = 3.4e-200
Identity = 341/466 (73.18%), Postives = 387/466 (83.05%), Query Frame = 0

Query: 143 MYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILCSDSKQSMYC 202
           MYLLFIKPEI TPSGLMARPVLTSYYKS++FRNRPFN+YNVYTSPD+TILC DSKQSMYC
Sbjct: 1   MYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFNKYNVYTSPDQTILCQDSKQSMYC 60

Query: 203 QLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHVSDWITDPSCRTAVS 262
           QLLCGLVQR  VLRVGAVFASAFLRA+KFLEDH+KEL  +IR G V+ WITD SCR +V 
Sbjct: 61  QLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKELCADIRTGTVTSWITDSSCRDSVL 120

Query: 263 LILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPTLEFYSGGLP 322
            IL  PN  LAD I ++C EKSWEGI+++IWPK KY+EVIVTGSMAQYIPTLEFYSGGLP
Sbjct: 121 SILNGPNQELADEIESECAEKSWEGILRRIWPKAKYVEVIVTGSMAQYIPTLEFYSGGLP 180

Query: 323 LVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNGGELQPHQSHCN-GG 382
           LVSTMYASSECYFGIN +PL  P+DVSYTLLPNMA+FEFLPV+    E     +H N   
Sbjct: 181 LVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYFEFLPVDDKSHEEIHFATHSNTDD 240

Query: 383 SAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFRFVHRRN 442
               + E   V+LV+VE+G YYE+V+TTFTGLYRYRVGDIL VTGFHNKAPQFRFV RRN
Sbjct: 241 DDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRVGDILKVTGFHNKAPQFRFVQRRN 300

Query: 443 VVLSIDTDKTNEDDLLKAITKAKL--LLEPLGFLLTEYTSYADTGSIPGHYVLFWEVKKR 502
           VVLSIDTDKT+E+DLL A+T+AKL  L  P   LLTEYTSYADT SIPGHYVLFWE+K R
Sbjct: 301 VVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTEYTSYADTSSIPGHYVLFWELKPR 360

Query: 503 GGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKHGAFDALMDFCV 562
             S +  +L+   ME+CCS VE  LD VYRRCRN+DKSIGPLEIRVV  G FD+LMDFCV
Sbjct: 361 -HSNDPPKLDDKTMEDCCSEVEDCLDYVYRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCV 420

Query: 563 SQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWEPFRVE 606
           SQGSS+NQYKTPRC+KS  A++ILDSRV+GRFFS++ P WEP  ++
Sbjct: 421 SQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRVPQWEPLGLD 465

BLAST of Lcy10g007160 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.9e-199
Identity = 334/590 (56.61%), Postives = 439/590 (74.41%), Query Frame = 0

Query: 14  LKLLEDLTQNARQIQEQVIHKILTQNANTEYLKTFINGHSPDVQLFKNTVPVVNYEDIKP 73
           LK +E++T+N   +QE+V+ +ILT+N+NTEYLK F      D + FK+ VPVV YED+KP
Sbjct: 24  LKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYEDLKP 83

Query: 74  YIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVMNKH 133
            I+RI+NG+ S I+S+ PITE LTSSGTS G+ K+MP+  EDLDR+   Y+LL+PVMN +
Sbjct: 84  EIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPVMNLY 143

Query: 134 VDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILC 193
           V GL++GKG+Y LF+K E  T  GL ARPVLTSYYKS +F+ RP++ YNVYTSP+E ILC
Sbjct: 144 VPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNEAILC 203

Query: 194 SDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELSDNIRNGHVSDWIT 253
           SDS QSMY Q+LCGL+ R EVLR+GAVFAS  LRAI FL+++WKEL+ +I  G +S  I 
Sbjct: 204 SDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLSSRIF 263

Query: 254 DPSCRTAVSLILAKPNAALADLIHAKCGEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPT 313
           DP+ +  +S IL KP+  LA+ +   C +++WEGII KIWP TKY++VIVTG+MAQYIPT
Sbjct: 264 DPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMAQYIPT 323

Query: 314 LEFYSGGLPLVSTMYASSECYFGINFDPLSKPSDVSYTLLPNMAFFEFLPVEKNGGELQP 373
           LE+YSGGLP+  TMYASSE YFGIN  P+ KPS+VSYT++PNMA+FEFL          P
Sbjct: 324 LEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFL----------P 383

Query: 374 HQSHCNGGSAQPIDEFETVDLVDVELGHYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQ 433
           H    +G +   +DE   V+L +VE+G  YELV+TT+ GLYRYRVGDIL VTGFHN APQ
Sbjct: 384 HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQ 443

Query: 434 FRFVHRRNVVLSIDTDKTNEDDLLKAITKAKLLLEPLGFLLTEYTSYADTGSIPGHYVLF 493
           F+F+ R+NV+LS+++DKT+E +L KA+  A  L    G  + EYTSYA+T +IPGHYV++
Sbjct: 444 FKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIY 503

Query: 494 WEVKKRGGSENLAELELDVMEECCSSVEQSLDSVYRRCRNKDKSIGPLEIRVVKHGAFDA 553
           WE+  R  S  L  +  +VM +CC  +E+SL+SVYR+ R  DKSIGPLEIRVV++G F+ 
Sbjct: 504 WELLGRDQSNAL--MSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEE 563

Query: 554 LMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSRKTPFWEPFR 604
           LMD+ +S+G+S+NQYK PRC+     +++LDSRVV   FS   P W P R
Sbjct: 564 LMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPER 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ876.8e-26272.25Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=... [more]
O818292.3e-20159.53Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
P0C0M32.5e-20058.80Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. jap... [more]
Q9LSQ41.6e-19958.28Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8GZ294.1e-19857.17Indole-3-acetic acid-amido synthetase GH3.15 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1ISD70.0e+0091.95indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1GWE70.0e+0091.30indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DHN60.0e+0087.12indole-3-acetic acid-amido synthetase GH3.17-like OS=Momordica charantia OX=3673... [more]
A0A5A7TMG22.3e-31086.02Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX... [more]
A0A1S3BYQ72.3e-31086.02indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_022980842.10.0e+0091.95indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima][more]
KAG6581972.10.0e+0091.79Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023528388.10.0e+0091.63indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo][more]
XP_022955484.10.0e+0091.30indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita moschata][more]
XP_022153513.10.0e+0087.12indole-3-acetic acid-amido synthetase GH3.17-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G28130.14.8e-26372.25Auxin-responsive GH3 family protein [more]
AT4G27260.11.6e-20259.53Auxin-responsive GH3 family protein [more]
AT5G54510.11.2e-20058.28Auxin-responsive GH3 family protein [more]
AT1G28130.23.4e-20073.18Auxin-responsive GH3 family protein [more]
AT4G37390.12.9e-19956.61Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 10..30
NoneNo IPR availablePANTHERPTHR31901:SF33INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.17coord: 3..599
IPR004993GH3 familyPFAMPF03321GH3coord: 17..577
e-value: 7.9E-206
score: 685.0
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 3..599

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g007160.1Lcy10g007160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity