Homology
BLAST of Lcy10g006560 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-152
Identity = 386/1155 (33.42%), Postives = 585/1155 (50.65%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAE+ L L N+ + +G +L L++G E EKL S I+AVL DA+E+Q
Sbjct: 1 MAEAFLQVLLENLTSFIG----DKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
A+E+W+ KL A Y++DD++ E EA+R +Q + F + FR ++
Sbjct: 61 AIENWLQKLNSAAYEVDDILGECKNEAIR----------FEQSRLGFYHPGIINFRHKIG 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKSVR 180
R++K++ E+L AI++++ +F F E++ T A R+ G + E +V GR ++ +
Sbjct: 121 RRMKEIMEKLDAISEERRKFHFLEKI--TERQAAAATRETGFVLTEPKVYGRDKEEDEIV 180
Query: 181 KLLVNS-STKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKIL 240
K+L+N+ + +++ V I+GMGGLGKT L Q ++ND+ +TKHF K+WVCVS++FD K L
Sbjct: 181 KILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRL 240
Query: 241 IEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDG 300
I+ II + P + S Q +L++ ++GK+YLLV+DDVWN++ EKW L+ +L G
Sbjct: 241 IKTIIGNIERSSPH-VEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVG 300
Query: 301 AKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIEI 360
A+G+ IL TTR E+V T Y L NL+ +S LLF M GQ++E N NL+ I
Sbjct: 301 ARGASILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLF--MQRAFGQQKE-ANPNLVAI 360
Query: 361 GKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSYE 420
GKEIV K GVPLA +T+G LL+ K+ + W +D E+ + E + L+LSY
Sbjct: 361 GKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDE--SSILPALRLSYH 420
Query: 421 HLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSKS 480
HL +L+QCF YCA+FPKD ++ K++LI W A GF+ S EL D+G + + EL +S
Sbjct: 421 HLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRS 480
Query: 481 FFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGRENDIDKRTHHFSLSSKSCEDWK 540
FFQE+ E + GN K+HDL+HDLA S+ FS S
Sbjct: 481 FFQEI-EAKSGN-TYFKIHDLIHDLATSL-------------------FSAS-------- 540
Query: 541 KLTRALSKARNLRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQLKWFETQQNAKFLESIIG 600
+SC N +R + +K
Sbjct: 541 --------------------ASCGN------------IREINVK---------------- 600
Query: 601 KFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRHLDLYGN 660
+KH + + + P+ + K +L L L Y
Sbjct: 601 DYKHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSY------------------------- 660
Query: 661 ENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSITQ 720
KL++LP I + +++RYLDLS NN LP+ + +
Sbjct: 661 ------------------------SKLEQLPSSIGDLLHLRYLDLSCNN-FRSLPERLCK 720
Query: 721 LNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFVL 780
L NL+TL + C +L LPK T +L +L+HL + C LT P +G LT L+T+ F++
Sbjct: 721 LQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC-PLTSTPPRIGLLTCLKTLGFFIV 780
Query: 781 GENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNNP-PN 840
G G L EL LN L G +SI +L+ + + + L K+ LQ L ++W+N PN
Sbjct: 781 GSKKGYQLGELKNLN-LCGSISITHLE--RVKNDTDAEANLSAKANLQSLSMSWDNDGPN 840
Query: 841 ELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGCLVNIELIHCKRL 900
+ +VL+ L+PHPNL+ + I + G + +W++ L ++++ + CK
Sbjct: 841 -----RYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNC 900
Query: 901 EHLPRFDQFPHLKYLRFED-LPRIEYIGNDNF---VSSSTFFPSLKKFIIIGMPKLKRWW 960
LP F + P L+ L ++ +EY+ D+ S+ FPSLKK I L
Sbjct: 901 LCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSL---- 960
Query: 961 KGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHIL-------------------ISWHA 1020
KG E E KFP L ++AI CPL + +S +
Sbjct: 961 KGLMKEEGEE--------KFPMLEEMAILYCPLFVFPTLSSVKKLEVHGNTNTRGLSSIS 985
Query: 1021 PLKSLT-IRDSEEVEFGAVPRENF---ENLSFLLLSRLSKLEYLPECLQHVTTLQTLVLS 1080
L +LT +R ++P E F NL FL L+ LP L + L+ L +
Sbjct: 1021 NLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIE 985
Query: 1081 NCENLKSLPE-WMENLTSLTKLEIGYCKKLNSLPEGIRHIHGLQSLRVIDCPILEERCKK 1126
+C++L+S PE +E LTSLT+L + YCK L LPEG++H+ L +L V CP +E+RC K
Sbjct: 1081 SCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEVEKRCDK 985
BLAST of Lcy10g006560 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 542.0 bits (1395), Expect = 1.6e-152
Identity = 387/1152 (33.59%), Postives = 587/1152 (50.95%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAE+ L L N L F + EL L++G EF+KL S I+AVL DA+E+Q
Sbjct: 1 MAEAFLQVLLDN----LTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
A+++W+ KL A Y++DD++D+ EA R KQ + + F ++
Sbjct: 61 AIKNWLQKLNVAAYEVDDILDDCKTEAAR----------FKQAVLGRYHPRTITFCYKVG 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKSVR 180
+++K++ E+L AI +++ F ER+I+ + ARR G + E +V GR ++ +
Sbjct: 121 KRMKEMMEKLDAIAEERRNFHLDERIIERQ----AARRQTGFVLTEPKVYGREKEEDEIV 180
Query: 181 KLLVNS-STKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKIL 240
K+L+N+ S +++ V+ I+GMGGLGKT L Q V+ND+ IT+HF LK+WVCVS++FD K L
Sbjct: 181 KILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRL 240
Query: 241 IEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDG 300
I+ I+ES GK + + LQ +L++ ++GK+Y LV+DDVWNE+ EKW L+ +L G
Sbjct: 241 IKAIVESIEGKSLGD-MDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIG 300
Query: 301 AKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIEI 360
A G+ ILITTR E++ T LY L NL++ + WLLFK+ E + L+EI
Sbjct: 301 ASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTE---TSPKLMEI 360
Query: 361 GKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSYE 420
GKEIV K GVPLA +T+G LL+ K+ + W +D E+ + E + L+LSY
Sbjct: 361 GKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDE--NSVLPALRLSYH 420
Query: 421 HLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSKS 480
HL +L+QCF YCA+FPKD +I+K+ LI W A F+ S EL D+G + + EL +S
Sbjct: 421 HLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLSKGNMELEDVGNEVWNELYLRS 480
Query: 481 FFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGRENDIDKRTHHFSLSSKSCEDWK 540
FFQE+ E + G KMHDL+HDLA S+ FS S+ S
Sbjct: 481 FFQEI-EVKSGK-TYFKMHDLIHDLATSM-------------------FSASASS----- 540
Query: 541 KLTRALSKARNLRTLFMV-NYSSCWNLDYDKFYHDHLRLRTLQLKWFETQQNAKFLESII 600
+ R ++ + +F+V NY ++ + + + + S+
Sbjct: 541 RSIRQINVKDDEDMMFIVTNYKDMMSIGFSEVV-------------------SSYSPSLF 600
Query: 601 GKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRHLDLYG 660
+F LR LN+SN + ++LP+ + L
Sbjct: 601 KRFVSLRVLNLSNSEFEQLPSSVGDL---------------------------------- 660
Query: 661 NENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSIT 720
+++RYLDLS N I LP +
Sbjct: 661 --------------------------------------VHLRYLDLS-GNKICSLPKRLC 720
Query: 721 QLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFV 780
+L NL+TL L C +L LPK T +L +L++L + C LT MP +G LT L+T+ FV
Sbjct: 721 KLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC-PLTSMPPRIGLLTCLKTLGYFV 780
Query: 781 LGENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNNPPN 840
+GE G L EL LN L+G +SI +L+ D++ + L K+ L L ++W+ P
Sbjct: 781 VGERKGYQLGELRNLN-LRGAISITHLERVK-NDMEAKEANLSAKANLHSLSMSWDRP-- 840
Query: 841 ELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGCLVNIELIHCKRL 900
+ +VL+ L+PHPNL+ + I + G L +W++ L +V+I + C+
Sbjct: 841 ----NRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENC 900
Query: 901 EHLPRFDQFPHLKYLRFED-LPRIEYIGNDNFVSSSTFFPSLKKFIIIGMPKLKRWWKGT 960
LP F + P L+ L +D +EY+ + F++ FPSL+K I G L KG
Sbjct: 901 SCLPPFGELPCLESLELQDGSVEVEYVEDSGFLTRRR-FPSLRKLHIGGFCNL----KGL 960
Query: 961 TSTESTEPTEGSFPIKFPCLSDLAIYGCPLHIL----------------------ISWHA 1020
+ E +FP L ++ I CP+ + IS +
Sbjct: 961 QRMKGAE--------QFPVLEEMKISDCPMFVFPTLSSVKKLEIWGEADAGGLSSISNLS 988
Query: 1021 PLKSLTIRDSEEV-EFGAVPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQTLVLSNCE 1080
L SL I + V +N ENL +L +S L L+ LP L + L+ L + C
Sbjct: 1021 TLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCY 988
Query: 1081 NLKSLP-EWMENLTSLTKLEIGYCKKLNSLPEGIRHIHGLQSLRVIDCPILEERCKKDTG 1126
L+SLP E +E L+SLT+L + +C L LPEG++H+ L SL++ CP L +RC+K G
Sbjct: 1081 ALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIG 988
BLAST of Lcy10g006560 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 531.2 bits (1367), Expect = 2.9e-149
Identity = 375/1135 (33.04%), Postives = 576/1135 (50.75%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAE+ + L N L +F + EL LL+G DEF++L S I+AVL DA+E+Q +
Sbjct: 1 MAEAFIQVLLDN----LTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNK 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
+E+W+ KL A Y++DD++DE+ +A R R + K + FR ++
Sbjct: 61 PLENWLQKLNAATYEVDDILDEYKTKATRFSQSEYGRYHPK----------VIPFRHKVG 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKSVR 180
+++ QV ++LKAI +++ F E++++ + RR+ G + E +V GR +K +
Sbjct: 121 KRMDQVMKKLKAIAEERKNFHLHEKIVERQ----AVRRETGSVLTEPQVYGRDKEKDEIV 180
Query: 181 KLLVNS-STKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKIL 240
K+L+N+ S ++V+ I+GMGGLGKT L Q V+ND+ +T+HF K+W+CVSE+FD K L
Sbjct: 181 KILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRL 240
Query: 241 IEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDG 300
I+ I+ES G+ + + LQ +L++ ++GK+YLLV+DDVWNE+ +KW L+ +L G
Sbjct: 241 IKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVG 300
Query: 301 AKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIEI 360
A G+ +L TTR E+V T Y L NL++ + WLLF M G +EE+ N NL+ I
Sbjct: 301 ASGASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLF--MQRAFGHQEEI-NPNLVAI 360
Query: 361 GKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSYE 420
GKEIV K GVPLA +T+G +L K+ +R W +D + + E + L+LSY
Sbjct: 361 GKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDE--SSILPALRLSYH 420
Query: 421 HLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSKS 480
L +LKQCF YCA+FPKD +++K+ LI W A GF+ S EL D+G++ + EL +S
Sbjct: 421 QLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRS 480
Query: 481 FFQEVTEDELGNIKA-CKMHDLMHDLACSITKNECVHGRENDIDKRTHHFSLSSKSCEDW 540
FFQE+ E+ + K KMHDL+HDLA S+ FS ++ S
Sbjct: 481 FFQEI---EVKDGKTYFKMHDLIHDLATSL-------------------FSANTSS---- 540
Query: 541 KKLTRALSKARNLRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQLKWFETQQNAKFLESII 600
N+R + +Y+ ++ + ++ +F T +
Sbjct: 541 ----------SNIREINKHSYTHMMSIGF------------AEVVFFYTLPP-------L 600
Query: 601 GKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRHLDLYG 660
KF LR LN+ + +LP+ I L ++LR+L+LYG
Sbjct: 601 EKFISLRVLNLGDSTFNKLPSSIGDL------------------------VHLRYLNLYG 660
Query: 661 NENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSIT 720
+ + LP +
Sbjct: 661 -------------------------------------------------SGMRSLPKQLC 720
Query: 721 QLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFV 780
+L NL+TL L +C+ L LPK T +L +L++L + +LT MP +G LT L+T+ +FV
Sbjct: 721 KLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFV 780
Query: 781 LGENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNNPPN 840
+G G L EL LN L G + I +L+ D + L K L L ++WNN
Sbjct: 781 VGRKKGYQLGELGNLN-LYGSIKISHLERVK-NDKDAKEANLSAKGNLHSLSMSWNNFGP 840
Query: 841 ELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGCLVNIELIHCKRL 900
+ + +VL+ L+PH NL + I + G+ L W++ L +V+I + + +
Sbjct: 841 HI----YESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNC 900
Query: 901 EHLPRFDQFPHLKYLRFE-DLPRIEYIGNDNFVSSSTF-----FPSLKKFIIIGMPKLKR 960
LP F P L+ L +EY+ + S F FPSL+K I L
Sbjct: 901 SCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSL-- 960
Query: 961 WWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHILISWHAPLKSLTI-RDSEEVEFG 1020
KG E E +FP L ++ I+ CP L S L SL I + F
Sbjct: 961 --KGLLKKEGEE--------QFPVLEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFP 966
Query: 1021 AVPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQTLVLSNCENLKSLP-EWMENLTSLT 1080
+N NL +L +SR + L+ LP L + L++L + C L+SLP E +E L+SLT
Sbjct: 1021 EEMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLT 966
Query: 1081 KLEIGYCKKLNSLPEGIRHIHGLQSLRVIDCPILEERCKKDTGEDWPKIAHIPQV 1126
+L + +C L LPEG++H+ L SL++ CP L +RC+K GEDW KI+HIP V
Sbjct: 1081 ELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIPNV 966
BLAST of Lcy10g006560 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 527.3 bits (1357), Expect = 4.2e-148
Identity = 371/1136 (32.66%), Postives = 579/1136 (50.97%), Query Frame = 0
Query: 13 IVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSHAVEDWVSKLKDA 72
++ L +F + EL LL+G DEF++L S I+AVL DA+E+Q +E+W+ KL A
Sbjct: 9 VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68
Query: 73 LYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMARKIKQVKERLKA 132
Y++DD++DE+ +A R R + K + FR ++ +++ QV ++L A
Sbjct: 69 TYEVDDILDEYKTKATRFLQSEYGRYHPK----------VIPFRHKVGKRMDQVMKKLNA 128
Query: 133 INDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKSVRKLLVN-SSTKDD 192
I +++ +F E++I+ + A R+ G + E +V GR +K + K+L+N +S
Sbjct: 129 IAEERKKFHLQEKIIERQ----AATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQK 188
Query: 193 IAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKILIEKIIESATGKK 252
++V+ I+GMGGLGKT L Q V+ND+ +T+ F K+W+C+S++F+ K LI+ I+ES GK
Sbjct: 189 LSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKS 248
Query: 253 PESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDGAKGSRILITTRS 312
S + + LQ +L++ ++GK+Y LV+DDVWNE+ KW L+ +L GA G+ +L TTR
Sbjct: 249 -LSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTTRL 308
Query: 313 EQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIEIGKEIVAKLKGVP 372
E+V T Y L NL+ + W LF M G +EE+ N NL+ IGKEIV K GVP
Sbjct: 309 EKVGSIMGTLQPYELSNLSPEDCWFLF--MQRAFGHQEEI-NPNLMAIGKEIVKKCGGVP 368
Query: 373 LAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSYEHLQANLKQCFLY 432
LA +T+G +L+ K+ +R W +D + + E + L+LSY HL +L+QCF+Y
Sbjct: 369 LAAKTLGGILRFKREEREWEHVRDSPIWNLPQDE--SSILPALRLSYHHLPLDLRQCFVY 428
Query: 433 CALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSKSFFQEVTEDELGN 492
CA+FPKD ++ K++LI W A GF+ S EL D+G + + EL +SFFQE+ E E G
Sbjct: 429 CAVFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEI-EVESGK 488
Query: 493 IKACKMHDLMHDLACSITKNECVHGRENDIDKRTHHFSLSSKSCEDWKKLTRALSKARNL 552
KMHDL+HDLA S+ FS ++ S N+
Sbjct: 489 -TYFKMHDLIHDLATSL-------------------FSANTSS--------------SNI 548
Query: 553 RTLFMVNYSSCWNLDYDKFYHDHLRLRTLQLKWFETQQNAKFLESIIGKFKHLRYLNISN 612
R + N +YD + + + + E + + S++ KF LR LN+ N
Sbjct: 549 REI---------NANYDGY--------MMSIGFAEVV--SSYSPSLLQKFVSLRVLNLRN 608
Query: 613 LDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRHLDLYGNENLEFLPDSMTE 672
+L +LP+ I L
Sbjct: 609 SNLNQLPSSIGDL----------------------------------------------- 668
Query: 673 LCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSITQLNNLETLLLSWC 732
+++RYLDLS N I LP + +L NL+TL L +C
Sbjct: 669 -------------------------VHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYC 728
Query: 733 SNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFVLGENNGGGLSELN 792
+L LPK T +L +L++L + C +LT P +G LT L++++ FV+G+ G L EL
Sbjct: 729 DSLSCLPKQTSKLGSLRNLLLDGC-SLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELK 788
Query: 793 ELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNNPPNELTIGNLDDVYER 852
LN L G +SI L D + L K+ L L L+W+ +
Sbjct: 789 NLN-LYGSISITKLDRVK-KDTDAKEANLSAKANLHSLCLSWD-------LDGKHRYDSE 848
Query: 853 VLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGCLVNIELIHCKRLEHLPRFDQFPHLK 912
VL+ L+PH NL+ + I+ + G++L +W++ L +V+I + C+ LP F + P L+
Sbjct: 849 VLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLE 908
Query: 913 YLRFE-DLPRIEYIGNDNFVSSSTFFPSLKKFIIIGMPKLKRWWKGTTSTESTEPTEGSF 972
L +EY+ DN FPSL+K +I L KG E +
Sbjct: 909 SLELHTGSADVEYV-EDNVHPGR--FPSLRKLVIWDFSNL----KGLLKMEGEK------ 968
Query: 973 PIKFPCLSDLAIYGCPLHIL------------------ISWHAPLKSLTIRD-SEEVEFG 1032
+FP L ++ Y CP+ ++ + + L++LT D S+ VE
Sbjct: 969 --QFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISDNVEAT 973
Query: 1033 AVPRENFE---NLSFLLLSRLSKLEYLPECLQHVTTLQTLVLSNCENLKSLP-EWMENLT 1092
++P E F+ NL +L +S L+ LP L + L++L C+ L+SLP E ++ LT
Sbjct: 1029 SLPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEGVKGLT 973
Query: 1093 SLTKLEIGYCKKLNSLPEGIRHIHGLQSLRVIDCPILEERCKKDTGEDWPKIAHIP 1124
SLT+L + C L LPEG++H+ L +L + CPI+ +RC++ GEDW KIAHIP
Sbjct: 1089 SLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCPIVFKRCERGIGEDWHKIAHIP 973
BLAST of Lcy10g006560 vs. ExPASy Swiss-Prot
Match:
Q9LRR5 (Putative disease resistance protein At3g14460 OS=Arabidopsis thaliana OX=3702 GN=At3g14460 PE=3 SV=1)
HSP 1 Score: 353.2 bits (905), Expect = 1.1e-95
Identity = 289/1012 (28.56%), Postives = 466/1012 (46.05%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDE--FEKLKDSVSAIKAVLLDAEEQQSV 60
MA S L + A +V ++ T +++ ++L G ++LK ++ VL DA+++
Sbjct: 1 MANSYLSSCANVMVERINT-SQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEH 60
Query: 61 SHAVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQ 120
V+ W++ +KDA + +D++DE EALRR+++ + + N +A R
Sbjct: 61 VREVKHWLTGIKDAFFQAEDILDELQTEALRRRVV------AEAGGLGGLFQNLMAGREA 120
Query: 121 MARKIKQVKERLKAINDDKT---QFSFSERLIDTRHDELR-ARRDIGHYIREEEVIGRSD 180
+ +KI+ E++ + + + + +TR + R A R + + ++GR +
Sbjct: 121 IQKKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASRSRPDDLPQGRLVGRVE 180
Query: 181 DKKSVRKLLVNSSTKDDI-----AVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVC 240
DK ++ LL++ D+I AVIS+VGM G+GKT L + V+ND +T+HFE+K+W+
Sbjct: 181 DKLALVNLLLSD---DEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWIS 240
Query: 241 VSEEFDIKILIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKW 300
F++ + + +++ T + + SLQ +L+K + GK++LLV+DD W+E+D +W
Sbjct: 241 AGINFNVFTVTKAVLQDITSSAVNT-EDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEW 300
Query: 301 VCLKRLLMDGAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMT----GLD 360
+ D +GS+I++TTRSE V+ + +Y ++ + W L + +
Sbjct: 301 ESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVG 360
Query: 361 GQEEELENSNLIEIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQE 420
+ELE IGK I + KG+PLA R I L+ K + +W +
Sbjct: 361 SINQELEG-----IGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNFSSYTNSIL 420
Query: 421 PLEEMQSILKLSYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFI-QSNDKDEL 480
P +LKLSY+ L LK+CF C++FPK + +++L+L W A + Q L
Sbjct: 421 P------VLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRL 480
Query: 481 IDIGEDYFLELLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGREN---DI 540
DIG DY +L+++SFFQ + + + MHDLM+DLA +++ + C ++ +I
Sbjct: 481 EDIGNDYLGDLVAQSFFQRLDI----TMTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEI 540
Query: 541 DKRTHHFSLSSKSCEDWKKLTRALSKARNLRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQ 600
T HFS S C D R++ A LRT+ N + L +LQ
Sbjct: 541 PSTTRHFSFSRSQC-DASVAFRSICGAEFLRTILPFNSPT--------------SLESLQ 600
Query: 601 LKWFETQQNAKFLESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETL 660
L K L ++ LR L++S+
Sbjct: 601 L-------TEKVLNPLLNALSGLRILSLSHY----------------------------- 660
Query: 661 PSDAKNWINLRHLDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRY 720
++ LPK +K +RY
Sbjct: 661 -------------------------------------------QITNLPKSLKGLKLLRY 720
Query: 721 LDLSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHM 780
LDLS + I+ LP+ + L NL+TLLLS C +L LPK+ LINL+ LD+ L M
Sbjct: 721 LDLS-STKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVG-TPLVEM 780
Query: 781 PKGLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQ 840
P G+ +L +LQ ++ FV+G +G GL EL EL+ L+G L I LQ A + D L+
Sbjct: 781 PPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFAS-EAKDAGLK 840
Query: 841 LKSWLQQLWLTWNNPPNELTIGNLDDV---YERVLDCLQPHPNLREISISRYEGVKLCNW 900
K +L L L W + G+ + + + VL L+PHP+L+ I Y+G W
Sbjct: 841 RKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKW 878
Query: 901 LSSDSLGCLVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYIGNDNFV----SSST 960
L S + ++ L C LP Q P LKYL E ++ +G D F S
Sbjct: 901 LGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGV 878
Query: 961 FFPSLKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCP 987
F SL+ GMP+ W E +G FPCL L I CP
Sbjct: 961 PFQSLQILKFYGMPRWDEW-------ICPELEDGI----FPCLQKLIIQRCP 878
BLAST of Lcy10g006560 vs. ExPASy TrEMBL
Match:
A0A6J1DKY9 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111021874 PE=4 SV=1)
HSP 1 Score: 1287.7 bits (3331), Expect = 0.0e+00
Identity = 694/1168 (59.42%), Postives = 851/1168 (72.86%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MA+SILFNLAG ++ KLG + QEL LL GV+ E +KLK +VSAI VLLDAEE+ S SH
Sbjct: 1 MADSILFNLAGTLINKLGXYVHQELGLLLGVNGELDKLKGTVSAIXKVLLDAEERSSKSH 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
A+EDW+SKLK Y++DDLIDEF YEALRRQ+MTRNRR TKQV IFFSKSNQ+AF +M
Sbjct: 61 ALEDWLSKLKGVFYEIDDLIDEFNYEALRRQVMTRNRRKTKQVRIFFSKSNQIAFSHKMG 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRH-DELRARRDIGHYIREEEVIGRSDDKKSV 180
KIK+++E+LK INDDKTQF SE +I+ D LR RD G YI EEEVIGR+ +KK +
Sbjct: 121 GKIKEIREKLKVINDDKTQFKLSECVINKSDVDILRNTRDPGDYIPEEEVIGRNYEKKEI 180
Query: 181 RKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKIL 240
LL+NS+ ++DIAV++IVGMGGLGKT + QSVY+DKM+TKHFEL+LWVC+SEEF++K++
Sbjct: 181 IDLLLNSNAREDIAVVAIVGMGGLGKTTIAQSVYSDKMMTKHFELRLWVCISEEFEVKVI 240
Query: 241 IEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDG 300
I+ IIE AT +K ESF QM LQ ELRK +DGKKYLLVMDDVWNE+ EKW LKRLLM G
Sbjct: 241 IQNIIEYATRRK-ESFPQMALLQTELRKLVDGKKYLLVMDDVWNEDHEKWNTLKRLLMGG 300
Query: 301 AKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMT-GLDGQEEELENSNLIE 360
AKGSRILITTR+ QVA TF++T YSLE LN+ NSWLLF+KM G +G E++ ENS L+E
Sbjct: 301 AKGSRILITTRTHQVANTFDSTSXYSLEELNDKNSWLLFEKMAFGGEGAEKDPENSKLVE 360
Query: 361 IGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSY 420
IGKEIV KLKG+PLAIRT+GRLL KKS W SFKDK+L+QI Q L++M SIL LSY
Sbjct: 361 IGKEIVTKLKGIPLAIRTVGRLLYYKKSTEFWSSFKDKKLYQIIEQGSLKDMLSILMLSY 420
Query: 421 EHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSK 480
HL NLKQCFLYCA+FPKDYEIQK+++I+QW AQ FI NDK + IDIGE+YF+ELLS+
Sbjct: 421 NHLPYNLKQCFLYCAMFPKDYEIQKNEVIMQWIAQSFIHPNDKYKFIDIGEEYFMELLSR 480
Query: 481 SFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHG-RENDIDKRTHHFSLSSKSCED 540
SFFQ+VT+++LG+I CKMHDLMHDLACSI +NECVH + KRTHH S S E+
Sbjct: 481 SFFQDVTKNDLGDIITCKMHDLMHDLACSIVENECVHDVNARTLSKRTHHVSFDLMSWEE 540
Query: 541 WKKLTRALSKARNLRTLFMVNYS-----SCWNLDYDKFYHDHLRLRTLQLKWFE-TQQNA 600
++L LS+A+NLRT F +Y+ + +N ++ +++ LRLRTL L+ +QN
Sbjct: 541 LERLPETLSEAKNLRTCFF-HYNQRRTDNNFNEIFESVFYNLLRLRTLDLQLLSFDKQNV 600
Query: 601 KFLESIIGKFKHLRYLNIS-NLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWIN 660
KFL+ I K KHLRYL I + N IT LYNLE L+ RL+ LP D KNW
Sbjct: 601 KFLK-CISKLKHLRYLEIQMYTKTEFFMNHITGLYNLEVFILR-CTRLKELPRDIKNWNE 660
Query: 661 LRHLDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSI 720
L+H+ LY NE +EFLP+S EL LETL+LH C KLKEL D++NWIN+RYLDLS ++ +
Sbjct: 661 LKHVYLYKNEIIEFLPNSFAELNNLETLVLHQCTKLKELLGDVRNWINLRYLDLS-SSGV 720
Query: 721 EFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTN 780
EFLPDSITQL NLETLLL +CS L+ELP++TK LINLKH+D+ C ALTHMPKGLG+LT+
Sbjct: 721 EFLPDSITQLKNLETLLLRFCSKLQELPRDTKNLINLKHVDLYGCWALTHMPKGLGELTD 780
Query: 781 LQTINEFVL-GENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQL 840
LQ +N FVL +NNGGGLSELNELNKL+G L+I L FC +DL KFL LKS LQ+L
Sbjct: 781 LQILNFFVLEKDNNGGGLSELNELNKLRGSLTINRLNFCTTSDLPTNAKFLLLKSSLQEL 840
Query: 841 WLTW---NNPPNELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGC 900
L W N+ NE TIG+LDD E VL+CL PH NLR + I +Y GVKLCNW+SS+ LGC
Sbjct: 841 VLEWEWDNDSVNEPTIGDLDDDCESVLECLHPHSNLRNLCIDKYRGVKLCNWVSSNFLGC 900
Query: 901 LVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYIGNDNFVSSSTFFPSLKKFIIIG 960
LV I+L CKRL+HLP+FDQFP+LK L +L IEYI ND++ SST FPSL++ I
Sbjct: 901 LVTIKLSGCKRLQHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCSSTLFPSLEELSIFC 960
Query: 961 MPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHILISWHAPLKSLTIRDSE 1020
MP LKRWWK ++++T FPI FPCLS L I+GCPL I+ HAP KSL + D
Sbjct: 961 MPNLKRWWKKGQTSKATSSQNVLFPIIFPCLSKLEIWGCPLLASITCHAPPKSLEMNDPI 1020
Query: 1021 EVEF-----GAVPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQTLVLSNCENLKSLPE 1080
E F V + FE+LS+L L+ + + +L LQHVTTLQ L L C+NL +LPE
Sbjct: 1021 EFHFDPDESDVVSWKCFESLSYLRLTHIDEWGFLSNWLQHVTTLQELTLEYCQNLTNLPE 1080
Query: 1081 WMENLTSLTKLE------------------------IGYCKKLNSLPEGIRHIHGLQSLR 1126
+ N TSL+KLE I +C L SLPEGIRH+H LQSL
Sbjct: 1081 SISNFTSLSKLEILKCTDLMSLPESIGNLTSLTELTIDFCPSLTSLPEGIRHLHCLQSLT 1140
BLAST of Lcy10g006560 vs. ExPASy TrEMBL
Match:
A0A6J1DHD4 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020497 PE=4 SV=1)
HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 675/1261 (53.53%), Postives = 846/1261 (67.09%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAESILF+LAGNI+T L +FARQEL LL GV DE +KL+D +S I+AV+LDAEEQQ H
Sbjct: 1 MAESILFDLAGNIITNLASFARQELALLCGVPDELDKLEDXLSDIQAVILDAEEQQYKRH 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRN--TKQVSIFFSKSNQVAFRLQ 120
A + W+SKLKDALYD+ DL DE +YEALRRQ ++N R QV +FFS+SN +AF L+
Sbjct: 61 AXKHWISKLKDALYDIYDLFDEISYEALRRQATSKNNRGKLCNQVRMFFSESNPIAFALK 120
Query: 121 MARKIKQVKERLKAINDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKS 180
KIK+++E LKAI+ DKTQF+FSER+ +T +D LR R+ I EEEVIGR+DDKK+
Sbjct: 121 TGHKIKEIRENLKAIDTDKTQFNFSERVSNTTNDGLRISREDASLILEEEVIGRNDDKKA 180
Query: 181 VRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 240
+ LL+ +T++DIAVISIVG GGLGKTAL QSVYND M+ KHF+ K WVCVS+EF++K+
Sbjct: 181 IINLLLKPNTEEDIAVISIVGTGGLGKTALAQSVYNDNMMDKHFKKKFWVCVSDEFELKV 240
Query: 241 LIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMD 300
+IEK+IESATGK+P+SFLQMDS Q ELRK IDGKKYLL+MDDVWNE+ EKWV L+RLL+
Sbjct: 241 MIEKMIESATGKRPDSFLQMDSSQSELRKLIDGKKYLLIMDDVWNEDYEKWVTLRRLLIC 300
Query: 301 GAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEE-ELENSNLI 360
GAKGSRILITTRSE+VAKTF+T+ LYSLE L+E +SWLLFKKM L G EE + ENSNL+
Sbjct: 301 GAKGSRILITTRSEKVAKTFDTSSLYSLEKLDEYHSWLLFKKMAFLKGSEEQDTENSNLV 360
Query: 361 EIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLS 420
+IGKEIVAKLKGVPLAIRTIGRLL K W SFKDKEL+QI +E +++M SILKLS
Sbjct: 361 QIGKEIVAKLKGVPLAIRTIGRLLCYNKKSNFWSSFKDKELYQIIEEESVKKMLSILKLS 420
Query: 421 YEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLS 480
Y L +NLKQCFLYCALFPKDY+I+K++++LQW AQGF+QSND + +D GEDYF++LLS
Sbjct: 421 YNSLPSNLKQCFLYCALFPKDYKIRKEEIVLQWIAQGFVQSNDGVKALDAGEDYFMKLLS 480
Query: 481 KSFFQEVTEDELGNIKACKMHDLMHDLACSITKNE--CVHG-RENDIDKRTHHFSLSSKS 540
SFFQ+V ++++G+I CKMHDLMHDLACSI +N VH ++KRTHH S S
Sbjct: 481 MSFFQDVRKNDVGDIITCKMHDLMHDLACSIVENNEIIVHDVNARTLNKRTHHVSFVLLS 540
Query: 541 CEDWKKLTRALSKARNLRTLFMVNYSSCWNLD-----------YDKFYHDHLRLRTLQLK 600
++ +KL + LSKARNLRT F+ Y+ D + +++ LRLRTL L
Sbjct: 541 GKELEKLPKTLSKARNLRTCFL-QYNQVGRRDDMLGKRKINKVFGSVFYNLLRLRTLDLH 600
Query: 601 WFETQQNAKFLESIIGKFKHLRYLNI---------------------------------- 660
N KFL+ I K K+LRYL I
Sbjct: 601 LSIDNHNVKFLK-CISKLKYLRYLKIKMIETTEYFPNSITGLYSLEVFILECKGLKELPR 660
Query: 661 ------------------------------------------------------------ 720
Sbjct: 661 DIKNWNKLKHLYLDGNENVWHRQDTYHYGNENVEFLPSSTVVLNNLETLVLQNFNNLKEF 720
Query: 721 ----------------SNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRH 780
N +++ LPN +++L NL++L L ++ LP D KN I+L+H
Sbjct: 721 PEDIKNLTNLTHLVMRGNKNIEYLPNSMSELNNLKSLILTRFEKVRELPQDIKNLIHLQH 780
Query: 781 LDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFL 840
LDL N N+EFLP+S+TELC+LET+IL GC KLKELP+D+KNWIN+R+LDLS + +EFL
Sbjct: 781 LDLQWNRNVEFLPNSITELCELETIILKGCTKLKELPRDVKNWINLRHLDLS-GSGVEFL 840
Query: 841 PDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQT 900
PDSIT NNLETLLL +C LKELP +TK+LINLKHLD+S C ALTHMPKGLG+LTNLQT
Sbjct: 841 PDSITHSNNLETLLLRYCEKLKELPGDTKKLINLKHLDLSGCRALTHMPKGLGELTNLQT 900
Query: 901 INEFVL--GENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWL 960
+N F+L GENNGGGLSELN LNKL+G L I+ LQFC+ DLQ K LQLKS LQQL L
Sbjct: 901 LNLFILGKGENNGGGLSELNGLNKLRGSLIIKGLQFCSAVDLQTNAKLLQLKSGLQQLML 960
Query: 961 TW---NNPPNELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGC-- 1020
W + NE T+G LD YERVL+CLQPH NL EI I+ Y GVKLCNWLSS S
Sbjct: 961 EWKWDKDSFNEPTVGALDGEYERVLECLQPHSNLPEIHINGYRGVKLCNWLSSSSYSFDG 1020
Query: 1021 LVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYIGNDNFVSSSTFFPSLKKFIIIG 1080
LV + + C+RL+HLPRF+QFP+LK+L DLP + ++ N+++ SS T F S + ++
Sbjct: 1021 LVRMTVSSCERLQHLPRFEQFPNLKHLHLRDLPCVRHMVNNDYPSSPTLFQSPEVLVVSD 1080
Query: 1081 MPKLKRWW-KGTTSTESTEPTEGSFPIKFPCLSDLAIYGCP-LHILISWHAPLKSLTIRD 1126
MP LKR W KGT S ++ FPI FPCLS+L I+ CP L ++ APL L+I +
Sbjct: 1081 MPNLKRGWKKGTKSAQNGV----LFPIIFPCLSELCIWKCPLLASSVTCDAPLDPLSISE 1140
BLAST of Lcy10g006560 vs. ExPASy TrEMBL
Match:
A0A1S4E349 (putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499944 PE=4 SV=1)
HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 657/1147 (57.28%), Postives = 819/1147 (71.40%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAE+IL+N +I+ LG+ A QE+ LW V+ E KLK S+SAI+AVL DAEEQQS ++
Sbjct: 1 MAEAILYNFTADIIFTLGSSALQEIGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
V+DWV KL+D LY++DDLIDE +Y+ LRRQ++ ++RR KQV I FSK ++
Sbjct: 61 QVKDWVLKLQDVLYEIDDLIDESSYQTLRRQVLAKHRRYRKQVRILFSKFKS---NWKIG 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRHDE--LRARRDIGHYIREEEVIGRSDDKKS 180
KIK++++RL+AIN+DK QFSF + +I+ R D+ LR RR+ +I E+EVIGR+DDK++
Sbjct: 121 HKIKEIRQRLQAINEDKNQFSFRKHVIERRDDDEGLRKRRETHSFILEDEVIGRNDDKEA 180
Query: 181 VRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 240
V LL+NS+TK+DIA++SIVGMGGLGKTAL +S+YN K I F LKLWVCVS+ FD++I
Sbjct: 181 VIDLLLNSNTKEDIAIVSIVGMGGLGKTALARSIYNHKRIMTRFSLKLWVCVSDAFDLEI 240
Query: 241 LIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMD 300
+++KIIESATGKK +LQ+DSLQ ELRK IDG KYL VMDDVWNE E+W+ LK LLM
Sbjct: 241 IVQKIIESATGKK-SGYLQIDSLQCELRKHIDGNKYLFVMDDVWNEKKEEWLHLKELLMA 300
Query: 301 GAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLD----GQEEELE-- 360
GAKGSRILITTRSEQVAKTF++TF++ L+ L+ NSWLLF+KMTGL+ QE E++
Sbjct: 301 GAKGSRILITTRSEQVAKTFDSTFVHPLQILDASNSWLLFQKMTGLEELSNNQEAEIDQT 360
Query: 361 NSNLIEIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQI--KGQEPLEEM 420
N NLI+IGKEI+++LKGVPL IRTIG LLK KS+R WLSFKDKEL+Q+ +GQ+ L+EM
Sbjct: 361 NLNLIQIGKEILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVLRQGQDALKEM 420
Query: 421 QSILKLSYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSN-DKD-ELIDIG 480
+ IL+LSY++L ANLKQCFLYCALFPKD+E+ K LIL WR+QG+IQ N +KD L+DIG
Sbjct: 421 KYILELSYKYLPANLKQCFLYCALFPKDFEVPKISLILLWRSQGYIQPNGNKDNNLVDIG 480
Query: 481 EDYFLELLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHG-RENDIDKRTHH 540
+ YF+ELLS+SFFQ+V +D+ G+I ACKMHD+MHDLACS+T NECVHG + N I+KRT H
Sbjct: 481 DGYFMELLSRSFFQDVKKDDSGDIVACKMHDMMHDLACSLTNNECVHGLKGNIINKRTRH 540
Query: 541 FSLSSKSCEDWKKLTRALSKARNLRTLFMVN----YSSCWNLDYDKFYHDHLRLRTLQLK 600
F L K + +L R LSKA +LRTLF S WN +++ + R RT+ L+
Sbjct: 541 FVLEDKDYHE-DQLMRPLSKATHLRTLFYRKEEDVIGSQWN--FEETFRSIFRFRTVHLE 600
Query: 601 WFETQQNAKFLESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPS 660
++ AK LE IGKFKHLR+L+I+ ++ LP+ ITKLY LE Q LE LP+
Sbjct: 601 GYDQNPKAKALE-FIGKFKHLRFLSITYSNIPYLPDSITKLYYLETFFFQCSHNLENLPN 660
Query: 661 DAKNWINLRHLDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLD 720
D N INL+HLDL N LEFLPDS+TELCKLE LIL GC KLK LPK+IKN N+R LD
Sbjct: 661 DVGNLINLKHLDLSHNSTLEFLPDSITELCKLEALILKGCEKLKRLPKNIKNCNNLRQLD 720
Query: 721 LSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPK 780
LS N IEFLPDSITQL NLETL+LSWC NLKELP++TKRLINLK L + C ALTHMPK
Sbjct: 721 LSHNERIEFLPDSITQLINLETLILSWCINLKELPEDTKRLINLKQLYLEICTALTHMPK 780
Query: 781 GLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFC-NIADLQMMDKFLQL 840
GLG+LTNLQT+N FVLG N GG L EL+ L KL+G+LSI +L+FC NI D +M KFLQL
Sbjct: 781 GLGELTNLQTLNTFVLGNNKGGELKELDGLTKLRGKLSIEHLEFCTNIVDHKMKGKFLQL 840
Query: 841 KSWLQQLWLTWNNPPNELTIGNLDDV-YERVLDCLQPHPNLREISISRYEGVKLCNWLSS 900
KS LQ+L L W + TIG L+DV YE VLDCLQPH NL++I I Y GV LCNW+ S
Sbjct: 841 KSGLQELKLHW----EKHTIGELEDVIYESVLDCLQPHSNLQKIYIDGYGGVNLCNWVVS 900
Query: 901 DS-LGCLVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYI--GNDNFVSSSTFFPS 960
LGCLV + L +C+RL HLP+FDQFP+LK+L DLP IEYI ND+ VSSST FPS
Sbjct: 901 PKFLGCLVTMRLCNCERLRHLPKFDQFPNLKHLYLIDLPNIEYIIANNDDSVSSSTNFPS 960
Query: 961 LKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHILISWHAPLK 1020
L+ II+ MPKL W KGTTST+S+ ++ +I P H +IS
Sbjct: 961 LENLIILNMPKLVSWCKGTTSTKSS-------------ITKSSIIIFPYHYMIS------ 1020
Query: 1021 SLTIRDSEEVEFGAVPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQTLVLSNCENLKS 1080
LQ+L+L NC+NLKS
Sbjct: 1021 ----------------------------------------------LQSLLLKNCQNLKS 1070
Query: 1081 LPEWMENLTSLTKLEIGYCKKLNSLPEGIRHIHGLQSLRVIDCPILEERCKKDTGEDWPK 1126
LPEW+ NLTSLT L I +CKKL LPEGIRH H L+ L + DCP+L ERCKK TGEDWPK
Sbjct: 1081 LPEWIGNLTSLTSLYISFCKKLTMLPEGIRHTHNLKYLAIYDCPMLRERCKKHTGEDWPK 1070
BLAST of Lcy10g006560 vs. ExPASy TrEMBL
Match:
A0A6J1DPI9 (LOW QUALITY PROTEIN: disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111021898 PE=4 SV=1)
HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 654/1238 (52.83%), Postives = 814/1238 (65.75%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MA+SILFNLAG ++TKLG++A QEL LL GV+DE EKLKD+VSAI+AVLLDAEEQ S SH
Sbjct: 1 MADSILFNLAGTLITKLGSYALQELGLLLGVNDELEKLKDTVSAIEAVLLDAEEQPSKSH 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
A++DW+SKLKD Y++DDLIDEF YEALRRQ+MTRNR TKQV IFFSKSNQ+AF +M
Sbjct: 61 ALKDWLSKLKDVFYEIDDLIDEFEYEALRRQVMTRNRTKTKQVRIFFSKSNQIAFSHKMV 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLID--TRHDELRARRDIGHYIREEEVIGRSDDKKS 180
KIK+++ERLK INDD+TQF SER+I+ T + LR RD G YI EEEVIGR+DDKK
Sbjct: 121 GKIKEIRERLKVINDDRTQFHLSERVINNPTVGNALRNIRDPGDYILEEEVIGRNDDKKV 180
Query: 181 VRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 240
+ L ++S+T+++IAV++ VGMGGLGKT L QSVYNDKM+ KHFELKLWVC+SEEF++K+
Sbjct: 181 IIDLQLSSNTRENIAVVATVGMGGLGKTTLAQSVYNDKMMAKHFELKLWVCISEEFEVKL 240
Query: 241 LIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMD 300
+I+ IIE ATG+KPESFLQM LQ +LRK +DGK+YLLVMDDVWNE+ EKW LKR L+
Sbjct: 241 IIQNIIEYATGEKPESFLQMALLQTKLRKLVDGKRYLLVMDDVWNEDREKWDTLKRFLIG 300
Query: 301 GAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIE 360
G KGS+ILITTRS QVA TF+ T L+ LE LN NSWLL
Sbjct: 301 GTKGSKILITTRSHQVANTFDPTSLHPLEELNNNNSWLL--------------------- 360
Query: 361 IGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSY 420
F+D+EL+QI Q L+ M SILKLS
Sbjct: 361 ----------------------------------FQDRELYQIMEQGGLKTMLSILKLSC 420
Query: 421 EHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSK 480
HL NLKQCF YCALFPKD+EI+KD++I+Q AQGFIQ NDKD+L+DIGEDYF+ELLSK
Sbjct: 421 NHLPPNLKQCFQYCALFPKDFEIRKDEVIMQGMAQGFIQPNDKDKLVDIGEDYFMELLSK 480
Query: 481 SFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGREND-----IDKRTHHFSLSSK 540
SFFQ+V +D LG+I CKMHDLMHD+ACS END ++KRT H SL
Sbjct: 481 SFFQDVKKDALGDIITCKMHDLMHDIACS----------ENDMNAPTLNKRTRHVSLDLT 540
Query: 541 SCEDWKKLTRALSKARNLRTLFM-------VNYSSCWNLDYDKFYHDHLRLRTLQLKWFE 600
S E+ +KL + L + RNLRT F+ + + +N + +++ LRLRTL L+
Sbjct: 541 SGEELEKLPKKLYETRNLRTCFLQYDLYNSIGSDNNFNKIFSSVFYNLLRLRTLGLQLII 600
Query: 601 TQQNAKFLESIIGKFKHLRYL--------------------------------------- 660
Q+ K L+ I KHLRYL
Sbjct: 601 DNQSVKLLK-CINNLKHLRYLKIEIITYTKFFLNSISGLYNLEVLIVRCTGLKEFPRDIK 660
Query: 661 NISNL---------DLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRHLDLYG 720
N++NL +++ PN I +L NLE L L+ +++ LP D +N I+L+ LDL
Sbjct: 661 NLTNLKHLELIGNPNIEFFPNSIIELNNLERLFLRGYNKVKELPQDIQNLIHLQRLDLGR 720
Query: 721 NENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSIT 780
N N+EFLPDS+TEL +L+TLIL C KLKELP+D++N IN+R+LDLS N +IEFLP SIT
Sbjct: 721 NSNIEFLPDSITELYELKTLILEECTKLKELPRDVQNLINLRHLDLS-NTAIEFLPSSIT 780
Query: 781 QLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFV 840
QL NLETLLL C LKELP++T+ LINL+HL + C ALTHMPKGLG+LT+LQ ++ F+
Sbjct: 781 QLKNLETLLLYLCDKLKELPEDTRNLINLRHLSICGCKALTHMPKGLGELTHLQRLDVFM 840
Query: 841 LG-ENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNNPP 900
LG +NNGGGL EL+ LNKL G L I LQFC+ DLQ KFLQ KS LQ L L WN+
Sbjct: 841 LGKDNNGGGLRELDGLNKLSGLLKIEGLQFCSTVDLQTNAKFLQRKSGLQYLQLEWNDDS 900
Query: 901 -NELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSD--SLGCLVNIELIH 960
NE TIG D YERVL+CL PH NL++I I Y+GVKLCNWLSS S G ++++E
Sbjct: 901 FNEPTIGASDYDYERVLECLPPHSNLQQICIRGYKGVKLCNWLSSTSYSFGGMIHMEFSS 960
Query: 961 CKRLEHLPRFDQFPHLKYLRFEDLPRIEYI-GNDNFVSSSTFFPSLKKFIIIGMPKLKRW 1020
C+RL HLP+FD FP+LK L DLP IEYI GN N+ SSTFFPSL+ FII MP LKRW
Sbjct: 961 CERLRHLPQFDLFPNLKVLCLHDLPSIEYIVGNHNYPCSSTFFPSLETFIISDMPNLKRW 1020
Query: 1021 W-KGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHILISWHAPLKSLTIRDSEEVEFGA 1080
W KG TST+S + FP+ FPCLS+L I+ CPL ++ AP+KSL I D EF
Sbjct: 1021 WRKGQTSTKSAQ-NRVLFPVVFPCLSELIIWKCPLLASVTCDAPMKSLEIND----EFDV 1080
Query: 1081 VPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQ-------------------------- 1123
V ++F++LS LL+S +S+ E+L CLQHVTTLQ
Sbjct: 1081 VSWKSFQSLSILLISSVSQWEFLSNCLQHVTTLQQLDTKDCPNLTSLPESIGNLTSLSKL 1140
BLAST of Lcy10g006560 vs. ExPASy TrEMBL
Match:
A0A6J1FNX2 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111445964 PE=4 SV=1)
HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 611/1251 (48.84%), Postives = 798/1251 (63.79%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAESILFN+A ++VTKLG+ A E QL W HD+ +KLK ++ A++AVL DAE+QQS SH
Sbjct: 54 MAESILFNVAASLVTKLGSPAITEFQLFWSFHDDLDKLKQTILAMEAVLHDAEKQQSNSH 113
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
AV++W+S++K+A YD+DDLIDE+ YE LR Q+M +R TK+V FS Q+ FR QM+
Sbjct: 114 AVKNWISRVKEAFYDVDDLIDEYTYETLRLQVMADGKRWTKEVRKVFSLPRQIEFRFQMS 173
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKSVR 180
KIK ++E+LKAI+DDK QF SE +I+TR DELR RR+ +I +++V+GR DKK++
Sbjct: 174 HKIKDIREKLKAIDDDKNQFHLSECMINTRDDELRKRRETSSFICDDDVVGRDGDKKAII 233
Query: 181 KLLVNS--STKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 240
L+N+ + K+++AVI+IVGMGGLGKTAL QSVYN++ I KHFEL LWVC+S++FD+K+
Sbjct: 234 DYLLNTYPNVKENVAVIAIVGMGGLGKTALAQSVYNNEGIRKHFELTLWVCISDDFDVKV 293
Query: 241 LIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMD 300
++EKI+ESAT K E LQMD LQ ELRK IDGKKYLL+MDDVWNE+ EKWV LKRLLM
Sbjct: 294 IVEKILESATKHKSE-ILQMDLLQSELRKNIDGKKYLLIMDDVWNEDYEKWVTLKRLLMG 353
Query: 301 GAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIE 360
GA GSRILITTR +VA+TF+TT Y+L L++ N+WLLFKKM G+E + E L++
Sbjct: 354 GASGSRILITTRHRRVAETFDTTSYYALGELDDKNAWLLFKKM-AFGGEEPKKE---LVD 413
Query: 361 IGKEIVAKLKGVPLAIRTIGRLLKGKKSKRN-WLSFKDKELHQIKGQ-EPLEEMQSILKL 420
IGKEIV KLKG+PLAIRTIGRLL KK + + W +FK+ EL ++ Q E + M SIL+L
Sbjct: 414 IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWSTFKENELSRVLEQREETQFMLSILEL 473
Query: 421 SYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELL 480
SY HL +NLKQCFLYCALFPKDY+IQKD++I QW A GF+QS + DIGEDYF+ELL
Sbjct: 474 SYNHLPSNLKQCFLYCALFPKDYDIQKDEVIRQWMAHGFVQSTGTIKPNDIGEDYFMELL 533
Query: 481 SKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGREND--IDKRTHHFSLSSKS 540
S+SFFQ+VT +E+G I CKMHDL+HDLACSI ++E V D + +RT H + +
Sbjct: 534 SRSFFQDVTRNEMGIIIYCKMHDLIHDLACSIVEDEYVSMDVKDKLVTERTRHILILKEF 593
Query: 541 CEDWKKLTRALSKARNLRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQLKWFETQQNAKFL 600
+ L + L KA+NL+TL + + + L LRTL++++ T +K L
Sbjct: 594 GIKLESL-KLLFKAKNLKTLIIDAVNEDCQKVVNLISTQFLCLRTLKVEFCYT-NTSKVL 653
Query: 601 ESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQY-------------------- 660
S IGK KHLRYL I L+ LPN ITKLYNLE L L +
Sbjct: 654 PSSIGKLKHLRYLRICQRKLEFLPNSITKLYNLEILILDWCECLQELPRDAKNWMKLRYL 713
Query: 661 -----------------LRRLET-----------LPSDAKNWINLRHLDLYGNENLEFLP 720
L LET LP DAKNW LR+L L GN+N++FLP
Sbjct: 714 SLDRNVNIKFLPDSIAALHNLETLILDGCQCLQELPRDAKNWKKLRYLSLVGNKNIKFLP 773
Query: 721 DSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSITQLNNLETL 780
DS+ L LETLIL GC L+ELP++ KNW+ +RYL L N +I+FLPD I L+NLETL
Sbjct: 774 DSIGALHNLETLILDGCQCLQELPREAKNWMKLRYLSLVRNENIKFLPDFIAALHNLETL 833
Query: 781 LLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFVLGENNGGG 840
+L CS L+ELPK+ K+ +NLKHL++S C LTHMPKGLG+LT+LQT+N FVL ++ G
Sbjct: 834 ILQDCSELRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIGCD 893
Query: 841 LSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNNPPNELTIGNLD 900
LSELN L+KL+G L+I L+ C +++K L LK +Q+L L WN ++ LD
Sbjct: 894 LSELNRLDKLEGSLTIEGLEVCT---FDVLEKSLPLKLGVQELELVWNYSEDQDMNIVLD 953
Query: 901 DVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGCLVNIELIHCKRLEHLPRFDQ 960
+ + LQPH NLR I I Y V+LC+WLSS++ LV+I L +C +L+ LP+FDQ
Sbjct: 954 NECKGGFKWLQPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFDQ 1013
Query: 961 FPHLKYLRFEDLPRIEYIGNDNFVSSSTFFPSLKKFIIIGMPKLKRWWKGTTSTESTEPT 1020
FP LKYL + LP IEYI N+ + SSS FFPSL++ I+ M LK WWKG TS ES+ P
Sbjct: 1014 FPFLKYLTLQSLPNIEYIDNNEYPSSSMFFPSLEELSIVEMLNLKGWWKGETSLESS-PN 1073
Query: 1021 EGSFPIKFPCLSDLAIYGCPLHILISWHAPLKSLT------------------------- 1080
SFPI L L I CP WHA LKSL+
Sbjct: 1074 NASFPIAMSRLCKLEIDACPHLASFPWHALLKSLSLHAISSQLLNVVFERTANHCVEECF 1133
Query: 1081 ------------------------------IRDSEEVEFGA-----------------VP 1126
I+D + ++ + V
Sbjct: 1134 STFVSFDKIDDLEFLPEGFFYHFTNLEDLEIKDCKNLQMSSALVQHPINSLVQHPINDVL 1193
BLAST of Lcy10g006560 vs. NCBI nr
Match:
XP_022154678.1 (putative disease resistance protein RGA4 [Momordica charantia])
HSP 1 Score: 1287.7 bits (3331), Expect = 0.0e+00
Identity = 694/1168 (59.42%), Postives = 851/1168 (72.86%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MA+SILFNLAG ++ KLG + QEL LL GV+ E +KLK +VSAI VLLDAEE+ S SH
Sbjct: 1 MADSILFNLAGTLINKLGXYVHQELGLLLGVNGELDKLKGTVSAIXKVLLDAEERSSKSH 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
A+EDW+SKLK Y++DDLIDEF YEALRRQ+MTRNRR TKQV IFFSKSNQ+AF +M
Sbjct: 61 ALEDWLSKLKGVFYEIDDLIDEFNYEALRRQVMTRNRRKTKQVRIFFSKSNQIAFSHKMG 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRH-DELRARRDIGHYIREEEVIGRSDDKKSV 180
KIK+++E+LK INDDKTQF SE +I+ D LR RD G YI EEEVIGR+ +KK +
Sbjct: 121 GKIKEIREKLKVINDDKTQFKLSECVINKSDVDILRNTRDPGDYIPEEEVIGRNYEKKEI 180
Query: 181 RKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKIL 240
LL+NS+ ++DIAV++IVGMGGLGKT + QSVY+DKM+TKHFEL+LWVC+SEEF++K++
Sbjct: 181 IDLLLNSNAREDIAVVAIVGMGGLGKTTIAQSVYSDKMMTKHFELRLWVCISEEFEVKVI 240
Query: 241 IEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDG 300
I+ IIE AT +K ESF QM LQ ELRK +DGKKYLLVMDDVWNE+ EKW LKRLLM G
Sbjct: 241 IQNIIEYATRRK-ESFPQMALLQTELRKLVDGKKYLLVMDDVWNEDHEKWNTLKRLLMGG 300
Query: 301 AKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMT-GLDGQEEELENSNLIE 360
AKGSRILITTR+ QVA TF++T YSLE LN+ NSWLLF+KM G +G E++ ENS L+E
Sbjct: 301 AKGSRILITTRTHQVANTFDSTSXYSLEELNDKNSWLLFEKMAFGGEGAEKDPENSKLVE 360
Query: 361 IGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSY 420
IGKEIV KLKG+PLAIRT+GRLL KKS W SFKDK+L+QI Q L++M SIL LSY
Sbjct: 361 IGKEIVTKLKGIPLAIRTVGRLLYYKKSTEFWSSFKDKKLYQIIEQGSLKDMLSILMLSY 420
Query: 421 EHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSK 480
HL NLKQCFLYCA+FPKDYEIQK+++I+QW AQ FI NDK + IDIGE+YF+ELLS+
Sbjct: 421 NHLPYNLKQCFLYCAMFPKDYEIQKNEVIMQWIAQSFIHPNDKYKFIDIGEEYFMELLSR 480
Query: 481 SFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHG-RENDIDKRTHHFSLSSKSCED 540
SFFQ+VT+++LG+I CKMHDLMHDLACSI +NECVH + KRTHH S S E+
Sbjct: 481 SFFQDVTKNDLGDIITCKMHDLMHDLACSIVENECVHDVNARTLSKRTHHVSFDLMSWEE 540
Query: 541 WKKLTRALSKARNLRTLFMVNYS-----SCWNLDYDKFYHDHLRLRTLQLKWFE-TQQNA 600
++L LS+A+NLRT F +Y+ + +N ++ +++ LRLRTL L+ +QN
Sbjct: 541 LERLPETLSEAKNLRTCFF-HYNQRRTDNNFNEIFESVFYNLLRLRTLDLQLLSFDKQNV 600
Query: 601 KFLESIIGKFKHLRYLNIS-NLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWIN 660
KFL+ I K KHLRYL I + N IT LYNLE L+ RL+ LP D KNW
Sbjct: 601 KFLK-CISKLKHLRYLEIQMYTKTEFFMNHITGLYNLEVFILR-CTRLKELPRDIKNWNE 660
Query: 661 LRHLDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSI 720
L+H+ LY NE +EFLP+S EL LETL+LH C KLKEL D++NWIN+RYLDLS ++ +
Sbjct: 661 LKHVYLYKNEIIEFLPNSFAELNNLETLVLHQCTKLKELLGDVRNWINLRYLDLS-SSGV 720
Query: 721 EFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTN 780
EFLPDSITQL NLETLLL +CS L+ELP++TK LINLKH+D+ C ALTHMPKGLG+LT+
Sbjct: 721 EFLPDSITQLKNLETLLLRFCSKLQELPRDTKNLINLKHVDLYGCWALTHMPKGLGELTD 780
Query: 781 LQTINEFVL-GENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQL 840
LQ +N FVL +NNGGGLSELNELNKL+G L+I L FC +DL KFL LKS LQ+L
Sbjct: 781 LQILNFFVLEKDNNGGGLSELNELNKLRGSLTINRLNFCTTSDLPTNAKFLLLKSSLQEL 840
Query: 841 WLTW---NNPPNELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGC 900
L W N+ NE TIG+LDD E VL+CL PH NLR + I +Y GVKLCNW+SS+ LGC
Sbjct: 841 VLEWEWDNDSVNEPTIGDLDDDCESVLECLHPHSNLRNLCIDKYRGVKLCNWVSSNFLGC 900
Query: 901 LVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYIGNDNFVSSSTFFPSLKKFIIIG 960
LV I+L CKRL+HLP+FDQFP+LK L +L IEYI ND++ SST FPSL++ I
Sbjct: 901 LVTIKLSGCKRLQHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCSSTLFPSLEELSIFC 960
Query: 961 MPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHILISWHAPLKSLTIRDSE 1020
MP LKRWWK ++++T FPI FPCLS L I+GCPL I+ HAP KSL + D
Sbjct: 961 MPNLKRWWKKGQTSKATSSQNVLFPIIFPCLSKLEIWGCPLLASITCHAPPKSLEMNDPI 1020
Query: 1021 EVEF-----GAVPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQTLVLSNCENLKSLPE 1080
E F V + FE+LS+L L+ + + +L LQHVTTLQ L L C+NL +LPE
Sbjct: 1021 EFHFDPDESDVVSWKCFESLSYLRLTHIDEWGFLSNWLQHVTTLQELTLEYCQNLTNLPE 1080
Query: 1081 WMENLTSLTKLE------------------------IGYCKKLNSLPEGIRHIHGLQSLR 1126
+ N TSL+KLE I +C L SLPEGIRH+H LQSL
Sbjct: 1081 SISNFTSLSKLEILKCTDLMSLPESIGNLTSLTELTIDFCPSLTSLPEGIRHLHCLQSLT 1140
BLAST of Lcy10g006560 vs. NCBI nr
Match:
XP_022152874.1 (disease resistance protein RGA2-like [Momordica charantia])
HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 675/1261 (53.53%), Postives = 846/1261 (67.09%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAESILF+LAGNI+T L +FARQEL LL GV DE +KL+D +S I+AV+LDAEEQQ H
Sbjct: 1 MAESILFDLAGNIITNLASFARQELALLCGVPDELDKLEDXLSDIQAVILDAEEQQYKRH 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRN--TKQVSIFFSKSNQVAFRLQ 120
A + W+SKLKDALYD+ DL DE +YEALRRQ ++N R QV +FFS+SN +AF L+
Sbjct: 61 AXKHWISKLKDALYDIYDLFDEISYEALRRQATSKNNRGKLCNQVRMFFSESNPIAFALK 120
Query: 121 MARKIKQVKERLKAINDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKS 180
KIK+++E LKAI+ DKTQF+FSER+ +T +D LR R+ I EEEVIGR+DDKK+
Sbjct: 121 TGHKIKEIRENLKAIDTDKTQFNFSERVSNTTNDGLRISREDASLILEEEVIGRNDDKKA 180
Query: 181 VRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 240
+ LL+ +T++DIAVISIVG GGLGKTAL QSVYND M+ KHF+ K WVCVS+EF++K+
Sbjct: 181 IINLLLKPNTEEDIAVISIVGTGGLGKTALAQSVYNDNMMDKHFKKKFWVCVSDEFELKV 240
Query: 241 LIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMD 300
+IEK+IESATGK+P+SFLQMDS Q ELRK IDGKKYLL+MDDVWNE+ EKWV L+RLL+
Sbjct: 241 MIEKMIESATGKRPDSFLQMDSSQSELRKLIDGKKYLLIMDDVWNEDYEKWVTLRRLLIC 300
Query: 301 GAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEE-ELENSNLI 360
GAKGSRILITTRSE+VAKTF+T+ LYSLE L+E +SWLLFKKM L G EE + ENSNL+
Sbjct: 301 GAKGSRILITTRSEKVAKTFDTSSLYSLEKLDEYHSWLLFKKMAFLKGSEEQDTENSNLV 360
Query: 361 EIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLS 420
+IGKEIVAKLKGVPLAIRTIGRLL K W SFKDKEL+QI +E +++M SILKLS
Sbjct: 361 QIGKEIVAKLKGVPLAIRTIGRLLCYNKKSNFWSSFKDKELYQIIEEESVKKMLSILKLS 420
Query: 421 YEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLS 480
Y L +NLKQCFLYCALFPKDY+I+K++++LQW AQGF+QSND + +D GEDYF++LLS
Sbjct: 421 YNSLPSNLKQCFLYCALFPKDYKIRKEEIVLQWIAQGFVQSNDGVKALDAGEDYFMKLLS 480
Query: 481 KSFFQEVTEDELGNIKACKMHDLMHDLACSITKNE--CVHG-RENDIDKRTHHFSLSSKS 540
SFFQ+V ++++G+I CKMHDLMHDLACSI +N VH ++KRTHH S S
Sbjct: 481 MSFFQDVRKNDVGDIITCKMHDLMHDLACSIVENNEIIVHDVNARTLNKRTHHVSFVLLS 540
Query: 541 CEDWKKLTRALSKARNLRTLFMVNYSSCWNLD-----------YDKFYHDHLRLRTLQLK 600
++ +KL + LSKARNLRT F+ Y+ D + +++ LRLRTL L
Sbjct: 541 GKELEKLPKTLSKARNLRTCFL-QYNQVGRRDDMLGKRKINKVFGSVFYNLLRLRTLDLH 600
Query: 601 WFETQQNAKFLESIIGKFKHLRYLNI---------------------------------- 660
N KFL+ I K K+LRYL I
Sbjct: 601 LSIDNHNVKFLK-CISKLKYLRYLKIKMIETTEYFPNSITGLYSLEVFILECKGLKELPR 660
Query: 661 ------------------------------------------------------------ 720
Sbjct: 661 DIKNWNKLKHLYLDGNENVWHRQDTYHYGNENVEFLPSSTVVLNNLETLVLQNFNNLKEF 720
Query: 721 ----------------SNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRH 780
N +++ LPN +++L NL++L L ++ LP D KN I+L+H
Sbjct: 721 PEDIKNLTNLTHLVMRGNKNIEYLPNSMSELNNLKSLILTRFEKVRELPQDIKNLIHLQH 780
Query: 781 LDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFL 840
LDL N N+EFLP+S+TELC+LET+IL GC KLKELP+D+KNWIN+R+LDLS + +EFL
Sbjct: 781 LDLQWNRNVEFLPNSITELCELETIILKGCTKLKELPRDVKNWINLRHLDLS-GSGVEFL 840
Query: 841 PDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQT 900
PDSIT NNLETLLL +C LKELP +TK+LINLKHLD+S C ALTHMPKGLG+LTNLQT
Sbjct: 841 PDSITHSNNLETLLLRYCEKLKELPGDTKKLINLKHLDLSGCRALTHMPKGLGELTNLQT 900
Query: 901 INEFVL--GENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWL 960
+N F+L GENNGGGLSELN LNKL+G L I+ LQFC+ DLQ K LQLKS LQQL L
Sbjct: 901 LNLFILGKGENNGGGLSELNGLNKLRGSLIIKGLQFCSAVDLQTNAKLLQLKSGLQQLML 960
Query: 961 TW---NNPPNELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGC-- 1020
W + NE T+G LD YERVL+CLQPH NL EI I+ Y GVKLCNWLSS S
Sbjct: 961 EWKWDKDSFNEPTVGALDGEYERVLECLQPHSNLPEIHINGYRGVKLCNWLSSSSYSFDG 1020
Query: 1021 LVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYIGNDNFVSSSTFFPSLKKFIIIG 1080
LV + + C+RL+HLPRF+QFP+LK+L DLP + ++ N+++ SS T F S + ++
Sbjct: 1021 LVRMTVSSCERLQHLPRFEQFPNLKHLHLRDLPCVRHMVNNDYPSSPTLFQSPEVLVVSD 1080
Query: 1081 MPKLKRWW-KGTTSTESTEPTEGSFPIKFPCLSDLAIYGCP-LHILISWHAPLKSLTIRD 1126
MP LKR W KGT S ++ FPI FPCLS+L I+ CP L ++ APL L+I +
Sbjct: 1081 MPNLKRGWKKGTKSAQNGV----LFPIIFPCLSELCIWKCPLLASSVTCDAPLDPLSISE 1140
BLAST of Lcy10g006560 vs. NCBI nr
Match:
XP_016902648.1 (PREDICTED: putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo])
HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 657/1147 (57.28%), Postives = 819/1147 (71.40%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MAE+IL+N +I+ LG+ A QE+ LW V+ E KLK S+SAI+AVL DAEEQQS ++
Sbjct: 1 MAEAILYNFTADIIFTLGSSALQEIGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
V+DWV KL+D LY++DDLIDE +Y+ LRRQ++ ++RR KQV I FSK ++
Sbjct: 61 QVKDWVLKLQDVLYEIDDLIDESSYQTLRRQVLAKHRRYRKQVRILFSKFKS---NWKIG 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLIDTRHDE--LRARRDIGHYIREEEVIGRSDDKKS 180
KIK++++RL+AIN+DK QFSF + +I+ R D+ LR RR+ +I E+EVIGR+DDK++
Sbjct: 121 HKIKEIRQRLQAINEDKNQFSFRKHVIERRDDDEGLRKRRETHSFILEDEVIGRNDDKEA 180
Query: 181 VRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 240
V LL+NS+TK+DIA++SIVGMGGLGKTAL +S+YN K I F LKLWVCVS+ FD++I
Sbjct: 181 VIDLLLNSNTKEDIAIVSIVGMGGLGKTALARSIYNHKRIMTRFSLKLWVCVSDAFDLEI 240
Query: 241 LIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMD 300
+++KIIESATGKK +LQ+DSLQ ELRK IDG KYL VMDDVWNE E+W+ LK LLM
Sbjct: 241 IVQKIIESATGKK-SGYLQIDSLQCELRKHIDGNKYLFVMDDVWNEKKEEWLHLKELLMA 300
Query: 301 GAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLD----GQEEELE-- 360
GAKGSRILITTRSEQVAKTF++TF++ L+ L+ NSWLLF+KMTGL+ QE E++
Sbjct: 301 GAKGSRILITTRSEQVAKTFDSTFVHPLQILDASNSWLLFQKMTGLEELSNNQEAEIDQT 360
Query: 361 NSNLIEIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQI--KGQEPLEEM 420
N NLI+IGKEI+++LKGVPL IRTIG LLK KS+R WLSFKDKEL+Q+ +GQ+ L+EM
Sbjct: 361 NLNLIQIGKEILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVLRQGQDALKEM 420
Query: 421 QSILKLSYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSN-DKD-ELIDIG 480
+ IL+LSY++L ANLKQCFLYCALFPKD+E+ K LIL WR+QG+IQ N +KD L+DIG
Sbjct: 421 KYILELSYKYLPANLKQCFLYCALFPKDFEVPKISLILLWRSQGYIQPNGNKDNNLVDIG 480
Query: 481 EDYFLELLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHG-RENDIDKRTHH 540
+ YF+ELLS+SFFQ+V +D+ G+I ACKMHD+MHDLACS+T NECVHG + N I+KRT H
Sbjct: 481 DGYFMELLSRSFFQDVKKDDSGDIVACKMHDMMHDLACSLTNNECVHGLKGNIINKRTRH 540
Query: 541 FSLSSKSCEDWKKLTRALSKARNLRTLFMVN----YSSCWNLDYDKFYHDHLRLRTLQLK 600
F L K + +L R LSKA +LRTLF S WN +++ + R RT+ L+
Sbjct: 541 FVLEDKDYHE-DQLMRPLSKATHLRTLFYRKEEDVIGSQWN--FEETFRSIFRFRTVHLE 600
Query: 601 WFETQQNAKFLESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPS 660
++ AK LE IGKFKHLR+L+I+ ++ LP+ ITKLY LE Q LE LP+
Sbjct: 601 GYDQNPKAKALE-FIGKFKHLRFLSITYSNIPYLPDSITKLYYLETFFFQCSHNLENLPN 660
Query: 661 DAKNWINLRHLDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLD 720
D N INL+HLDL N LEFLPDS+TELCKLE LIL GC KLK LPK+IKN N+R LD
Sbjct: 661 DVGNLINLKHLDLSHNSTLEFLPDSITELCKLEALILKGCEKLKRLPKNIKNCNNLRQLD 720
Query: 721 LSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPK 780
LS N IEFLPDSITQL NLETL+LSWC NLKELP++TKRLINLK L + C ALTHMPK
Sbjct: 721 LSHNERIEFLPDSITQLINLETLILSWCINLKELPEDTKRLINLKQLYLEICTALTHMPK 780
Query: 781 GLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFC-NIADLQMMDKFLQL 840
GLG+LTNLQT+N FVLG N GG L EL+ L KL+G+LSI +L+FC NI D +M KFLQL
Sbjct: 781 GLGELTNLQTLNTFVLGNNKGGELKELDGLTKLRGKLSIEHLEFCTNIVDHKMKGKFLQL 840
Query: 841 KSWLQQLWLTWNNPPNELTIGNLDDV-YERVLDCLQPHPNLREISISRYEGVKLCNWLSS 900
KS LQ+L L W + TIG L+DV YE VLDCLQPH NL++I I Y GV LCNW+ S
Sbjct: 841 KSGLQELKLHW----EKHTIGELEDVIYESVLDCLQPHSNLQKIYIDGYGGVNLCNWVVS 900
Query: 901 DS-LGCLVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYI--GNDNFVSSSTFFPS 960
LGCLV + L +C+RL HLP+FDQFP+LK+L DLP IEYI ND+ VSSST FPS
Sbjct: 901 PKFLGCLVTMRLCNCERLRHLPKFDQFPNLKHLYLIDLPNIEYIIANNDDSVSSSTNFPS 960
Query: 961 LKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHILISWHAPLK 1020
L+ II+ MPKL W KGTTST+S+ ++ +I P H +IS
Sbjct: 961 LENLIILNMPKLVSWCKGTTSTKSS-------------ITKSSIIIFPYHYMIS------ 1020
Query: 1021 SLTIRDSEEVEFGAVPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQTLVLSNCENLKS 1080
LQ+L+L NC+NLKS
Sbjct: 1021 ----------------------------------------------LQSLLLKNCQNLKS 1070
Query: 1081 LPEWMENLTSLTKLEIGYCKKLNSLPEGIRHIHGLQSLRVIDCPILEERCKKDTGEDWPK 1126
LPEW+ NLTSLT L I +CKKL LPEGIRH H L+ L + DCP+L ERCKK TGEDWPK
Sbjct: 1081 LPEWIGNLTSLTSLYISFCKKLTMLPEGIRHTHNLKYLAIYDCPMLRERCKKHTGEDWPK 1070
BLAST of Lcy10g006560 vs. NCBI nr
Match:
XP_022154711.1 (LOW QUALITY PROTEIN: disease resistance protein RGA2-like [Momordica charantia])
HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 654/1238 (52.83%), Postives = 814/1238 (65.75%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSH 60
MA+SILFNLAG ++TKLG++A QEL LL GV+DE EKLKD+VSAI+AVLLDAEEQ S SH
Sbjct: 1 MADSILFNLAGTLITKLGSYALQELGLLLGVNDELEKLKDTVSAIEAVLLDAEEQPSKSH 60
Query: 61 AVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMA 120
A++DW+SKLKD Y++DDLIDEF YEALRRQ+MTRNR TKQV IFFSKSNQ+AF +M
Sbjct: 61 ALKDWLSKLKDVFYEIDDLIDEFEYEALRRQVMTRNRTKTKQVRIFFSKSNQIAFSHKMV 120
Query: 121 RKIKQVKERLKAINDDKTQFSFSERLID--TRHDELRARRDIGHYIREEEVIGRSDDKKS 180
KIK+++ERLK INDD+TQF SER+I+ T + LR RD G YI EEEVIGR+DDKK
Sbjct: 121 GKIKEIRERLKVINDDRTQFHLSERVINNPTVGNALRNIRDPGDYILEEEVIGRNDDKKV 180
Query: 181 VRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 240
+ L ++S+T+++IAV++ VGMGGLGKT L QSVYNDKM+ KHFELKLWVC+SEEF++K+
Sbjct: 181 IIDLQLSSNTRENIAVVATVGMGGLGKTTLAQSVYNDKMMAKHFELKLWVCISEEFEVKL 240
Query: 241 LIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMD 300
+I+ IIE ATG+KPESFLQM LQ +LRK +DGK+YLLVMDDVWNE+ EKW LKR L+
Sbjct: 241 IIQNIIEYATGEKPESFLQMALLQTKLRKLVDGKRYLLVMDDVWNEDREKWDTLKRFLIG 300
Query: 301 GAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIE 360
G KGS+ILITTRS QVA TF+ T L+ LE LN NSWLL
Sbjct: 301 GTKGSKILITTRSHQVANTFDPTSLHPLEELNNNNSWLL--------------------- 360
Query: 361 IGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSY 420
F+D+EL+QI Q L+ M SILKLS
Sbjct: 361 ----------------------------------FQDRELYQIMEQGGLKTMLSILKLSC 420
Query: 421 EHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKDELIDIGEDYFLELLSK 480
HL NLKQCF YCALFPKD+EI+KD++I+Q AQGFIQ NDKD+L+DIGEDYF+ELLSK
Sbjct: 421 NHLPPNLKQCFQYCALFPKDFEIRKDEVIMQGMAQGFIQPNDKDKLVDIGEDYFMELLSK 480
Query: 481 SFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGREND-----IDKRTHHFSLSSK 540
SFFQ+V +D LG+I CKMHDLMHD+ACS END ++KRT H SL
Sbjct: 481 SFFQDVKKDALGDIITCKMHDLMHDIACS----------ENDMNAPTLNKRTRHVSLDLT 540
Query: 541 SCEDWKKLTRALSKARNLRTLFM-------VNYSSCWNLDYDKFYHDHLRLRTLQLKWFE 600
S E+ +KL + L + RNLRT F+ + + +N + +++ LRLRTL L+
Sbjct: 541 SGEELEKLPKKLYETRNLRTCFLQYDLYNSIGSDNNFNKIFSSVFYNLLRLRTLGLQLII 600
Query: 601 TQQNAKFLESIIGKFKHLRYL--------------------------------------- 660
Q+ K L+ I KHLRYL
Sbjct: 601 DNQSVKLLK-CINNLKHLRYLKIEIITYTKFFLNSISGLYNLEVLIVRCTGLKEFPRDIK 660
Query: 661 NISNL---------DLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRHLDLYG 720
N++NL +++ PN I +L NLE L L+ +++ LP D +N I+L+ LDL
Sbjct: 661 NLTNLKHLELIGNPNIEFFPNSIIELNNLERLFLRGYNKVKELPQDIQNLIHLQRLDLGR 720
Query: 721 NENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSIT 780
N N+EFLPDS+TEL +L+TLIL C KLKELP+D++N IN+R+LDLS N +IEFLP SIT
Sbjct: 721 NSNIEFLPDSITELYELKTLILEECTKLKELPRDVQNLINLRHLDLS-NTAIEFLPSSIT 780
Query: 781 QLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFV 840
QL NLETLLL C LKELP++T+ LINL+HL + C ALTHMPKGLG+LT+LQ ++ F+
Sbjct: 781 QLKNLETLLLYLCDKLKELPEDTRNLINLRHLSICGCKALTHMPKGLGELTHLQRLDVFM 840
Query: 841 LG-ENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNNPP 900
LG +NNGGGL EL+ LNKL G L I LQFC+ DLQ KFLQ KS LQ L L WN+
Sbjct: 841 LGKDNNGGGLRELDGLNKLSGLLKIEGLQFCSTVDLQTNAKFLQRKSGLQYLQLEWNDDS 900
Query: 901 -NELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSD--SLGCLVNIELIH 960
NE TIG D YERVL+CL PH NL++I I Y+GVKLCNWLSS S G ++++E
Sbjct: 901 FNEPTIGASDYDYERVLECLPPHSNLQQICIRGYKGVKLCNWLSSTSYSFGGMIHMEFSS 960
Query: 961 CKRLEHLPRFDQFPHLKYLRFEDLPRIEYI-GNDNFVSSSTFFPSLKKFIIIGMPKLKRW 1020
C+RL HLP+FD FP+LK L DLP IEYI GN N+ SSTFFPSL+ FII MP LKRW
Sbjct: 961 CERLRHLPQFDLFPNLKVLCLHDLPSIEYIVGNHNYPCSSTFFPSLETFIISDMPNLKRW 1020
Query: 1021 W-KGTTSTESTEPTEGSFPIKFPCLSDLAIYGCPLHILISWHAPLKSLTIRDSEEVEFGA 1080
W KG TST+S + FP+ FPCLS+L I+ CPL ++ AP+KSL I D EF
Sbjct: 1021 WRKGQTSTKSAQ-NRVLFPVVFPCLSELIIWKCPLLASVTCDAPMKSLEIND----EFDV 1080
Query: 1081 VPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQ-------------------------- 1123
V ++F++LS LL+S +S+ E+L CLQHVTTLQ
Sbjct: 1081 VSWKSFQSLSILLISSVSQWEFLSNCLQHVTTLQQLDTKDCPNLTSLPESIGNLTSLSKL 1140
BLAST of Lcy10g006560 vs. NCBI nr
Match:
XP_031736581.1 (putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothetical protein Csa_006537 [Cucumis sativus])
HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 662/1172 (56.48%), Postives = 810/1172 (69.11%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFA-RQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVS 60
MAE+ILFNL +I+ KLG+ A RQ L GV D+F+KL S+SAI+AVL DAEE+Q
Sbjct: 1 MAEAILFNLTADIIFKLGSSALRQFGSLRGGVKDDFDKLWHSLSAIQAVLHDAEEKQFKD 60
Query: 61 HAVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQM 120
HAVE WVS+LKD LY++DDLIDEF+Y+ LRRQ++ NR KQV FSK ++
Sbjct: 61 HAVEVWVSRLKDVLYEIDDLIDEFSYQILRRQVLQSNR---KQVRTLFSK---FITNWKI 120
Query: 121 ARKIKQVKERLKAINDDKTQFSFSERLIDTRHDE---LRARRDIGHYIREEEVIGRSDDK 180
KIK++ +RL+ IN+DK QFSF + +I+ R D+ LR RR+ +I E+EVIGR+DDK
Sbjct: 121 GHKIKEISQRLQNINEDKIQFSFCKHVIERRDDDDEGLRKRRETHSFILEDEVIGRNDDK 180
Query: 181 KSVRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDI 240
++V LL+NS+TK+DIA++SIVGM G GKTAL Q +YN K I F+LK+WVCVS+EFD+
Sbjct: 181 EAVIDLLLNSNTKEDIAIVSIVGMPGFGKTALAQFIYNHKRIMTQFQLKIWVCVSDEFDL 240
Query: 241 KILIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLL 300
KI I+KIIESATGKKP+S LQMD LQ ELRKQIDGKKYL+VMDDVWNE EKW+ LKRLL
Sbjct: 241 KITIQKIIESATGKKPKSLLQMDPLQCELRKQIDGKKYLIVMDDVWNEKKEKWLHLKRLL 300
Query: 301 MDGAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLD----GQEEELE 360
M GAKGSRILITTRSEQVAKTF++TF++ L+ L+ NSWLLF+KM GL+ QE EL+
Sbjct: 301 MGGAKGSRILITTRSEQVAKTFDSTFVHLLQILDASNSWLLFQKMIGLEEHSNNQEIELD 360
Query: 361 --NSNLIEIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQI--KGQEPLE 420
NSNLI+IG EIV+ L+GVPL IRTIG LLK KS+R WLSFK+KEL+Q+ +GQ+ L+
Sbjct: 361 QKNSNLIQIGMEIVSTLRGVPLLIRTIGGLLKDNKSERFWLSFKNKELYQVLGRGQDALK 420
Query: 421 EMQSILKLSYEHL-QANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSN----DKDE 480
E+Q L+LSY++L +NLKQCFLYCALFPKDY I+KD+LIL WRAQGFIQ N D
Sbjct: 421 EIQLFLELSYKYLPSSNLKQCFLYCALFPKDYRIKKDELILLWRAQGFIQQNGNNDDNSS 480
Query: 481 LIDIGEDYFLELLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHG-RENDID 540
L+DIGEDYF+ELLS+SFFQEV +++ G+I CKMHDLMHDLACSIT NECV G + N ID
Sbjct: 481 LVDIGEDYFMELLSRSFFQEVEKNDFGDIITCKMHDLMHDLACSITNNECVRGLKGNVID 540
Query: 541 KRTHHFSLSSKSCEDWKKLTRALSKARNLRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQL 600
KRTHH S S ED +L +LSKA +LRTLF+ + S NL ++ +H+ +LRTL L
Sbjct: 541 KRTHHLSFEKVSHED--QLMGSLSKATHLRTLFIQDVCSRCNL--EETFHNIFQLRTLHL 600
Query: 601 KWFETQQNAKFLESIIGKFKHLRYLNISN-LDLKRLPNCITKLYNLEALTLQYLRRLETL 660
+ + AK + I K KHLRYL++ N + LP+ I +LYNLE Q L+ L
Sbjct: 601 NLYSPTKFAKTWK-FISKLKHLRYLHLKNSFCVTYLPDSILELYNLETFIFQ-SSLLKKL 660
Query: 661 PSDAKNWINLRHLDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRY 720
PS+ N INL+HLDL + NLEFLPDS+T+L KLE LILHG
Sbjct: 661 PSNVGNLINLKHLDLSSHLNLEFLPDSITKLYKLEALILHG------------------- 720
Query: 721 LDLSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHM 780
CSNLKELPK TKRLINLK L + C+ALTHM
Sbjct: 721 -----------------------------CSNLKELPKYTKRLINLKSLVLYGCSALTHM 780
Query: 781 PKGLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFC-NIADLQMMDKFL 840
PKGL ++TNLQT+ FVLG+N GG L EL L KL+G LSI++L+ C +I D QM K L
Sbjct: 781 PKGLSEMTNLQTLTTFVLGKNIGGELKELEGLTKLRGGLSIKHLESCTSIVDQQMKSKLL 840
Query: 841 QLKSWLQQLWLTWNNPPNELTIGNLDDV-YERVLDCLQPHPNLREISISRYEGVKLCNWL 900
QLKS LQ+L L W P ++ L+DV YE VLDCLQPH NL+EI I Y GV LCNW+
Sbjct: 841 QLKSGLQKLELQWKKP--KIGDDQLEDVMYESVLDCLQPHSNLKEIRIDGYGGVNLCNWV 900
Query: 901 SSD-SLGCLVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEY--IGNDNFVSSSTFF 960
SS+ SLGCLV I L CKRL HL R DQFP+LKYL ++LP IEY + ND+ VSSST F
Sbjct: 901 SSNKSLGCLVTIYLYRCKRLRHLFRLDQFPNLKYLTLQNLPNIEYMIVDNDDSVSSSTIF 960
Query: 961 PSLKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYG-CPLHILISWHA 1020
P LKKF I MPKL W K +TST+S PT + FP LS L I G C LH+L WHA
Sbjct: 961 PCLKKFTISKMPKLVSWCKDSTSTKS--PT-----VIFPHLSSLMIRGPCRLHMLKYWHA 1020
Query: 1021 P-LKSLTIRDSEEVEFGAVPRENFENLSFLLLSRLSKLEYLPECLQH-VTTLQTLVLSNC 1080
P LK L I DSE+ E VP + +ENL+ L L LS++EYLPEC QH +T+LQ L LS C
Sbjct: 1021 PKLKLLQISDSED-ELNVVPLKIYENLTSLFLHNLSRVEYLPECWQHYMTSLQLLYLSKC 1080
Query: 1081 ENLKSLPEW------------------------MENLTSLTKLEIGYCKKLNSLPEGIRH 1122
ENLKSLP W ++NLTSLT L+I YCK L LPEGI+H
Sbjct: 1081 ENLKSLPGWIGNLTSLTGLKISTCDKLTMLPEEIDNLTSLTNLDISYCKNLAFLPEGIQH 1102
BLAST of Lcy10g006560 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 353.2 bits (905), Expect = 7.7e-97
Identity = 289/1012 (28.56%), Postives = 466/1012 (46.05%), Query Frame = 0
Query: 1 MAESILFNLAGNIVTKLGTFARQELQLLWGVHDE--FEKLKDSVSAIKAVLLDAEEQQSV 60
MA S L + A +V ++ T +++ ++L G ++LK ++ VL DA+++
Sbjct: 1 MANSYLSSCANVMVERINT-SQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEH 60
Query: 61 SHAVEDWVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQ 120
V+ W++ +KDA + +D++DE EALRR+++ + + N +A R
Sbjct: 61 VREVKHWLTGIKDAFFQAEDILDELQTEALRRRVV------AEAGGLGGLFQNLMAGREA 120
Query: 121 MARKIKQVKERLKAINDDKT---QFSFSERLIDTRHDELR-ARRDIGHYIREEEVIGRSD 180
+ +KI+ E++ + + + + +TR + R A R + + ++GR +
Sbjct: 121 IQKKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASRSRPDDLPQGRLVGRVE 180
Query: 181 DKKSVRKLLVNSSTKDDI-----AVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVC 240
DK ++ LL++ D+I AVIS+VGM G+GKT L + V+ND +T+HFE+K+W+
Sbjct: 181 DKLALVNLLLSD---DEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWIS 240
Query: 241 VSEEFDIKILIEKIIESATGKKPESFLQMDSLQDELRKQIDGKKYLLVMDDVWNENDEKW 300
F++ + + +++ T + + SLQ +L+K + GK++LLV+DD W+E+D +W
Sbjct: 241 AGINFNVFTVTKAVLQDITSSAVNT-EDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEW 300
Query: 301 VCLKRLLMDGAKGSRILITTRSEQVAKTFETTFLYSLENLNECNSWLLFKKMT----GLD 360
+ D +GS+I++TTRSE V+ + +Y ++ + W L + +
Sbjct: 301 ESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVG 360
Query: 361 GQEEELENSNLIEIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQE 420
+ELE IGK I + KG+PLA R I L+ K + +W +
Sbjct: 361 SINQELEG-----IGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNFSSYTNSIL 420
Query: 421 PLEEMQSILKLSYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFI-QSNDKDEL 480
P +LKLSY+ L LK+CF C++FPK + +++L+L W A + Q L
Sbjct: 421 P------VLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRL 480
Query: 481 IDIGEDYFLELLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGREN---DI 540
DIG DY +L+++SFFQ + + + MHDLM+DLA +++ + C ++ +I
Sbjct: 481 EDIGNDYLGDLVAQSFFQRLDI----TMTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEI 540
Query: 541 DKRTHHFSLSSKSCEDWKKLTRALSKARNLRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQ 600
T HFS S C D R++ A LRT+ N + L +LQ
Sbjct: 541 PSTTRHFSFSRSQC-DASVAFRSICGAEFLRTILPFNSPT--------------SLESLQ 600
Query: 601 LKWFETQQNAKFLESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETL 660
L K L ++ LR L++S+
Sbjct: 601 L-------TEKVLNPLLNALSGLRILSLSHY----------------------------- 660
Query: 661 PSDAKNWINLRHLDLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRY 720
++ LPK +K +RY
Sbjct: 661 -------------------------------------------QITNLPKSLKGLKLLRY 720
Query: 721 LDLSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHM 780
LDLS + I+ LP+ + L NL+TLLLS C +L LPK+ LINL+ LD+ L M
Sbjct: 721 LDLS-STKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVG-TPLVEM 780
Query: 781 PKGLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFCNIADLQMMDKFLQ 840
P G+ +L +LQ ++ FV+G +G GL EL EL+ L+G L I LQ A + D L+
Sbjct: 781 PPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFAS-EAKDAGLK 840
Query: 841 LKSWLQQLWLTWNNPPNELTIGNLDDV---YERVLDCLQPHPNLREISISRYEGVKLCNW 900
K +L L L W + G+ + + + VL L+PHP+L+ I Y+G W
Sbjct: 841 RKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKW 878
Query: 901 LSSDSLGCLVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYIGNDNFV----SSST 960
L S + ++ L C LP Q P LKYL E ++ +G D F S
Sbjct: 901 LGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGV 878
Query: 961 FFPSLKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCP 987
F SL+ GMP+ W E +G FPCL L I CP
Sbjct: 961 PFQSLQILKFYGMPRWDEW-------ICPELEDGI----FPCLQKLIIQRCP 878
HSP 2 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 48/165 (29.09%), Postives = 74/165 (44.85%), Query Frame = 0
Query: 974 FPCLSDLAIYGCPLHILISW---HAP--LKSLTIRDSEEVEF--GAVPRENFENLSFLL- 1033
+P L +L I C H L S+ H P LK+L IRD +++ F P ++ L +L
Sbjct: 1115 YPNLHELLIIAC--HSLESFPGSHPPTTLKTLYIRDCKKLNFTESLQPTRSYSQLEYLFI 1174
Query: 1034 -----------LSRLSKLEYLP--ECLQHVT------------TLQTLVLSNCENLKSLP 1093
LS KL L +C T L++L + +C NL++ P
Sbjct: 1175 GSSCSNLVNFPLSLFPKLRSLSIRDCESFKTFSIHAGLGDDRIALESLEIRDCPNLETFP 1234
Query: 1094 EWMENLTSLTKLEIGYCKKLNSLPEGIRHIHGLQSLRVIDCPILE 1106
+ L+ + + CKKL +LPE + + L SL +I CP +E
Sbjct: 1235 QGGLPTPKLSSMLLSNCKKLQALPEKLFGLTSLLSLFIIKCPEIE 1277
BLAST of Lcy10g006560 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 334.0 bits (855), Expect = 4.8e-91
Identity = 310/1105 (28.05%), Postives = 513/1105 (46.43%), Query Frame = 0
Query: 20 FARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSHAVEDWVSKLKDALYDLDDL 79
F R+EL + E+L ++ I AVL+DAEE+Q + VE WV++L+D +Y +D
Sbjct: 29 FKRRELN-----ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDA 88
Query: 80 IDEFAYEALRRQI--------MTRNRRNTKQVSIFFSKSNQVAFRLQMARKIKQVKERLK 139
+D+ A EALR I R R + F +++ + ++++V RL+
Sbjct: 89 LDDIATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNSE-----HLETRLEKVTIRLE 148
Query: 140 AINDDKTQFSFSERLIDTRHDELRARRDIGHYIREEEVIGRSDDKKSVRKLLVNSSTKDD 199
+ + E + R + E EV GR DDK + + L+ + KD+
Sbjct: 149 RLASQRNILGLKELTAMIP----KQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDN 208
Query: 200 -IAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKILIEKIIESATGK 259
I V++IVG+GG+GKT L Q +YND+ + +F K+W VSEEFD+ + +K+ ES T
Sbjct: 209 GITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVT-S 268
Query: 260 KPESFLQMDSLQDELRKQIDGK--KYLLVMDDVWNENDEKWVCLKRLLMDGAKGSRILIT 319
+P F +D LQ +L++++ G +LLV+DD+WNEN W L++ + A+GS+IL+T
Sbjct: 269 RPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVT 328
Query: 320 TRSEQVAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELENSNLIEIGKEIVAKLK 379
TRS++VA +++L+ L++ + W LF K + G +E N + ++ + IV K +
Sbjct: 329 TRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKT--VFGNQEPCLNREIGDLAERIVHKCR 388
Query: 380 GVPLAIRTIGRLLKGKKSKRNWLSFKDKELHQIKGQEPLEEMQSILKLSYEHLQANLKQC 439
G+PLA++T+G +L+ + W + + + + +L++SY +L A+LK+C
Sbjct: 389 GLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADK--SNLLPVLRVSYYYLPAHLKRC 448
Query: 440 FLYCALFPKDYEIQKDDLILQWRAQGFI-QSNDKDELIDIGEDYFLELLSKSFFQEVTED 499
F YC++FPK + +KD ++L W A+GF+ Q+ L ++G +YF EL S+S Q+
Sbjct: 449 FAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTR 508
Query: 500 ELGNIKACKMHDLMHDLACSIT---KNECVHGRENDIDKRTHHFSLSSKSCEDWKKLTRA 559
+ MHD +++LA + ++ G + + +RT + S + + + A
Sbjct: 509 YI-------MHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEF-EA 568
Query: 560 LSKARNLRT---LFMVNYSSCWNLDY---DKFYHDHLRLRTLQLKWFETQQNAKFLESII 619
L + + LRT L + N S LD +K RLR L L ++ A+
Sbjct: 569 LREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKI---ARLPPDFF 628
Query: 620 GKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWINLRHLDLYG 679
H R+L++S +L++LP + +YNL+ L L Y L+ LP+D N INLR+LDL G
Sbjct: 629 KNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG 688
Query: 680 NENLEFLPDSMTELCKLETLILHGCGKLKELPKDIKNWINIRYLDLSWNNSIEFLPDSIT 739
+ L +P L L+TL + D S + + L D
Sbjct: 689 TK-LRQMPRRFGRLKSLQTL----------------TTFFVSASDGSRISELGGLHDLHG 748
Query: 740 QLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNALTHMPKGLGQLTNLQTINEFV 799
+L +E + ++ E N+K+ +L+ +D W +
Sbjct: 749 KLKIVELQRVVDVADAAEANLNSKK--HLREIDFVWRTGSSS------------------ 808
Query: 800 LGENNGGGLSELNE---LNKLKGRLSIRNLQFCNIADLQMMDKFLQLKSWLQQLWLTWNN 859
ENN NE KL+ I L + D WL+ +
Sbjct: 809 -SENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPD------------WLSDPS 868
Query: 860 PPNELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKLCNWLSSDSLGCLVNIELIHC 919
+ I + Y L L P L+E+ IS G++ S+G
Sbjct: 869 FSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQ--------SIGRKFYFSDQQL 928
Query: 920 KRLEHLPRFDQFPHLKYLRFEDLPR-IEYIGNDNFVSSSTFFPSLKKFIIIGMPKLKRWW 979
+ + P F L+ LRF++LP E++ D V+ FPSLKK I+ P+L
Sbjct: 929 RDQDQQP----FRSLETLRFDNLPDWQEWL--DVRVTRGDLFPSLKKLFILRCPEL---- 988
Query: 980 KGTTSTESTEPTEGSFPIKFPCLSDLAIYGC------PLHILISWHAPLKSLTIRDSEE- 1039
G+ P P L L IY C P H S+ L++L+I+ S +
Sbjct: 989 ------------TGTLPTFLPSLISLHIYKCGLLDFQPDHHEYSYR-NLQTLSIKSSCDT 1001
Query: 1040 -VEFGAVPRENFENLSFLLLSR---LSKLEYLPECLQHVTTLQTLVLSNCENLKSLPEWM 1089
V+F P +F NL L + + L LE E L+ L+ L +++C+NL+ LP
Sbjct: 1049 LVKF---PLNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLP--- 1001
BLAST of Lcy10g006560 vs. TAIR 10
Match:
AT1G58602.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 275.0 bits (702), Expect = 2.7e-73
Identity = 317/1220 (25.98%), Postives = 535/1220 (43.85%), Query Frame = 0
Query: 14 VTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSHAVEDWVSKLKDAL 73
V KL E L GV D+ +LK ++ +K+ L DA+ ++ S V V ++KD +
Sbjct: 10 VNKLWDLLSHEYTLFQGVEDQVAELKSDLNLLKSFLKDADAKKHTSALVRYCVEEIKDIV 69
Query: 74 YDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMARKIKQVKERLKAI 133
YD +D+++ F + + + R++ K+++ V R ++A I V +R+ +
Sbjct: 70 YDAEDVLETFVQKE-KLGTTSGIRKHIKRLTCI------VPDRREIALYIGHVSKRITRV 129
Query: 134 NDDKTQFSFSERLIDTRHDELRAR-RDIGHYIREEEVIGRSDDKKSVRKLLVNSSTKDDI 193
D F + ++D LR R R+I ++ G +++V+KL+ +D+
Sbjct: 130 IRDMQSFGVQQMIVDDYMHPLRNREREIRRTFPKDNESGFVALEENVKKLVGYFVEEDNY 189
Query: 194 AVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKILIEKII------ES 253
V+SI GMGGLGKT L + V+N M+TK F+ WV VS++F +K + + I+ E
Sbjct: 190 QVVSITGMGGLGKTTLARQVFNHDMVTKKFDKLAWVSVSQDFTLKNVWQNILGDLKPKEE 249
Query: 254 ATGKKPESFLQMD--SLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDGAKGSR 313
T ++ + L+M +LQ EL + ++ K L+V+DD+W + D W +K + KG +
Sbjct: 250 ETKEEEKKILEMTEYTLQRELYQLLEMSKSLIVLDDIWKKED--WEVIKP-IFPPTKGWK 309
Query: 314 ILITTRSEQ-VAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELE-NSNLIEIGKE 373
+L+T+R+E VA T F + E L +SW LF+++ E E + + ++G++
Sbjct: 310 LLLTSRNESIVAPTNTKYFNFKPECLKTDDSWKLFQRIAFPINDASEFEIDEEMEKLGEK 369
Query: 374 IVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDK-ELHQIKGQEPLEEMQS-----ILKL 433
++ G+PLAI+ +G +L K + +W + H + G+ + + +L L
Sbjct: 370 MIEHCGGLPLAIKVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNSCNYVLSL 429
Query: 434 SYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKD-ELI-DIGEDYFLE 493
S+E L + LK CFLY A FP+DYEI+ ++L W A+ Q D E+I D+G+ Y E
Sbjct: 430 SFEELPSYLKHCFLYLAHFPEDYEIKVENLSYYWAAEEIFQPRHYDGEIIRDVGDVYIEE 489
Query: 494 LLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNE-CVHGRENDIDKRTHHFSLSSK 553
L+ ++ + + + C +HD+M ++ K E + N +++S+
Sbjct: 490 LVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSNPPSTANFQSTVTSR 549
Query: 554 SCEDWKKLTRALSKARN---LRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQLKWFETQQN 613
T + K N LR+L +V S WN+ F L LR L L + +
Sbjct: 550 RLVYQYPTTLHVEKDINNPKLRSLVVVTLGS-WNMAGSSFTRLEL-LRVLDL--VQAKLK 609
Query: 614 AKFLESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWI- 673
L S IGK HLRYL++ ++ +P Y+L L L L S N++
Sbjct: 610 GGKLASCIGKLIHLRYLSLEYAEVTHIP------YSLGNLKLLIYLNLHISLSSRSNFVP 669
Query: 674 NLRHLDLYGNENLEF--LPD--------SMTELCKLETLILHGCGKLKELPKDIKNWINI 733
N+ L G + L + LP ++ L KLET L +D++ + +
Sbjct: 670 NV----LMGMQELRYLALPSLIERKTKLELSNLVKLET--LENFSTKNSSLEDLRGMVRL 729
Query: 734 RYL--DLSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNA 793
R L +L S+E L SI L LE L + + K K + + HL
Sbjct: 730 RTLTIELIEETSLETLAASIGGLKYLEKLEIDDLGS-KMRTKEAGIVFDFVHLKR--LRL 789
Query: 794 LTHMPKGLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFCNIAD--LQM 853
+MP+ L++ L+ LQ C + + + +
Sbjct: 790 ELYMPR--------------------------LSKEQHFPSHLTTLYLQHCRLEEDPMPI 849
Query: 854 MDKFLQLKSWLQQLWLTWNNPPNELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKL 913
++K LQLK EL +G+ ++++ P L+++SIS G+K
Sbjct: 850 LEKLLQLK---------------ELELGHKSFSGKKMVCSSCGFPQLQKLSIS---GLKE 909
Query: 914 C-NW-LSSDSLGCLVNIELIHCKRL-----EHLPRFDQFPHLKYLRFED-LPRIEYIGND 973
+W + S+ L+ + + C++L EHLP LK ED +P +E + +
Sbjct: 910 WEDWKVEESSMPLLLTLNIFDCRKLKQLPDEHLPSHLTAISLKKCGLEDPIPTLERLVHL 969
Query: 974 NFVSSSTF-----------FPSLKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPC 1033
+S S FP L K + + L+ W +GS P
Sbjct: 970 KELSLSELCGRIMVCTGGGFPQLHKLDLSELDGLEEW----------IVEDGS----MPR 1029
Query: 1034 LSDLAIYGC-PLHILISWHAPLKSLTIRDSEEVEFGAVPRE-NFENLSFLLLSRLSKLEY 1093
L L I C L L + L++L + + EE E G + ++ + L L + KL
Sbjct: 1030 LHTLEIRRCLKLKKLPNGFPQLQNLHLTEVEEWEEGMIVKQGSMPLLHTLYIWHCPKLPG 1089
Query: 1094 LPECLQHVTTLQTL----------VLSNCENLKSLP------------------------ 1128
H+TT+ L +L +LK++
Sbjct: 1090 EQHFPSHLTTVFLLGMYVEEDPMRILEKLLHLKNVSLFQSFSGKRMVCSGGGFPQLQKLS 1136
BLAST of Lcy10g006560 vs. TAIR 10
Match:
AT1G58602.2 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 275.0 bits (702), Expect = 2.7e-73
Identity = 317/1220 (25.98%), Postives = 535/1220 (43.85%), Query Frame = 0
Query: 14 VTKLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSHAVEDWVSKLKDAL 73
V KL E L GV D+ +LK ++ +K+ L DA+ ++ S V V ++KD +
Sbjct: 10 VNKLWDLLSHEYTLFQGVEDQVAELKSDLNLLKSFLKDADAKKHTSALVRYCVEEIKDIV 69
Query: 74 YDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSKSNQVAFRLQMARKIKQVKERLKAI 133
YD +D+++ F + + + R++ K+++ V R ++A I V +R+ +
Sbjct: 70 YDAEDVLETFVQKE-KLGTTSGIRKHIKRLTCI------VPDRREIALYIGHVSKRITRV 129
Query: 134 NDDKTQFSFSERLIDTRHDELRAR-RDIGHYIREEEVIGRSDDKKSVRKLLVNSSTKDDI 193
D F + ++D LR R R+I ++ G +++V+KL+ +D+
Sbjct: 130 IRDMQSFGVQQMIVDDYMHPLRNREREIRRTFPKDNESGFVALEENVKKLVGYFVEEDNY 189
Query: 194 AVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKILIEKII------ES 253
V+SI GMGGLGKT L + V+N M+TK F+ WV VS++F +K + + I+ E
Sbjct: 190 QVVSITGMGGLGKTTLARQVFNHDMVTKKFDKLAWVSVSQDFTLKNVWQNILGDLKPKEE 249
Query: 254 ATGKKPESFLQMD--SLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRLLMDGAKGSR 313
T ++ + L+M +LQ EL + ++ K L+V+DD+W + D W +K + KG +
Sbjct: 250 ETKEEEKKILEMTEYTLQRELYQLLEMSKSLIVLDDIWKKED--WEVIKP-IFPPTKGWK 309
Query: 314 ILITTRSEQ-VAKTFETTFLYSLENLNECNSWLLFKKMTGLDGQEEELE-NSNLIEIGKE 373
+L+T+R+E VA T F + E L +SW LF+++ E E + + ++G++
Sbjct: 310 LLLTSRNESIVAPTNTKYFNFKPECLKTDDSWKLFQRIAFPINDASEFEIDEEMEKLGEK 369
Query: 374 IVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDK-ELHQIKGQEPLEEMQS-----ILKL 433
++ G+PLAI+ +G +L K + +W + H + G+ + + +L L
Sbjct: 370 MIEHCGGLPLAIKVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNSCNYVLSL 429
Query: 434 SYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKD-ELI-DIGEDYFLE 493
S+E L + LK CFLY A FP+DYEI+ ++L W A+ Q D E+I D+G+ Y E
Sbjct: 430 SFEELPSYLKHCFLYLAHFPEDYEIKVENLSYYWAAEEIFQPRHYDGEIIRDVGDVYIEE 489
Query: 494 LLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNE-CVHGRENDIDKRTHHFSLSSK 553
L+ ++ + + + C +HD+M ++ K E + N +++S+
Sbjct: 490 LVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSNPPSTANFQSTVTSR 549
Query: 554 SCEDWKKLTRALSKARN---LRTLFMVNYSSCWNLDYDKFYHDHLRLRTLQLKWFETQQN 613
T + K N LR+L +V S WN+ F L LR L L + +
Sbjct: 550 RLVYQYPTTLHVEKDINNPKLRSLVVVTLGS-WNMAGSSFTRLEL-LRVLDL--VQAKLK 609
Query: 614 AKFLESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRRLETLPSDAKNWI- 673
L S IGK HLRYL++ ++ +P Y+L L L L S N++
Sbjct: 610 GGKLASCIGKLIHLRYLSLEYAEVTHIP------YSLGNLKLLIYLNLHISLSSRSNFVP 669
Query: 674 NLRHLDLYGNENLEF--LPD--------SMTELCKLETLILHGCGKLKELPKDIKNWINI 733
N+ L G + L + LP ++ L KLET L +D++ + +
Sbjct: 670 NV----LMGMQELRYLALPSLIERKTKLELSNLVKLET--LENFSTKNSSLEDLRGMVRL 729
Query: 734 RYL--DLSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHLDMSWCNA 793
R L +L S+E L SI L LE L + + K K + + HL
Sbjct: 730 RTLTIELIEETSLETLAASIGGLKYLEKLEIDDLGS-KMRTKEAGIVFDFVHLKR--LRL 789
Query: 794 LTHMPKGLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFCNIAD--LQM 853
+MP+ L++ L+ LQ C + + + +
Sbjct: 790 ELYMPR--------------------------LSKEQHFPSHLTTLYLQHCRLEEDPMPI 849
Query: 854 MDKFLQLKSWLQQLWLTWNNPPNELTIGNLDDVYERVLDCLQPHPNLREISISRYEGVKL 913
++K LQLK EL +G+ ++++ P L+++SIS G+K
Sbjct: 850 LEKLLQLK---------------ELELGHKSFSGKKMVCSSCGFPQLQKLSIS---GLKE 909
Query: 914 C-NW-LSSDSLGCLVNIELIHCKRL-----EHLPRFDQFPHLKYLRFED-LPRIEYIGND 973
+W + S+ L+ + + C++L EHLP LK ED +P +E + +
Sbjct: 910 WEDWKVEESSMPLLLTLNIFDCRKLKQLPDEHLPSHLTAISLKKCGLEDPIPTLERLVHL 969
Query: 974 NFVSSSTF-----------FPSLKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPC 1033
+S S FP L K + + L+ W +GS P
Sbjct: 970 KELSLSELCGRIMVCTGGGFPQLHKLDLSELDGLEEW----------IVEDGS----MPR 1029
Query: 1034 LSDLAIYGC-PLHILISWHAPLKSLTIRDSEEVEFGAVPRE-NFENLSFLLLSRLSKLEY 1093
L L I C L L + L++L + + EE E G + ++ + L L + KL
Sbjct: 1030 LHTLEIRRCLKLKKLPNGFPQLQNLHLTEVEEWEEGMIVKQGSMPLLHTLYIWHCPKLPG 1089
Query: 1094 LPECLQHVTTLQTL----------VLSNCENLKSLP------------------------ 1128
H+TT+ L +L +LK++
Sbjct: 1090 EQHFPSHLTTVFLLGMYVEEDPMRILEKLLHLKNVSLFQSFSGKRMVCSGGGFPQLQKLS 1136
BLAST of Lcy10g006560 vs. TAIR 10
Match:
AT1G58848.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 248.8 bits (634), Expect = 2.0e-65
Identity = 296/1167 (25.36%), Postives = 508/1167 (43.53%), Query Frame = 0
Query: 9 LAGNIVT----KLGTFARQELQLLWGVHDEFEKLKDSVSAIKAVLLDAEEQQSVSHAVED 68
+AG +++ L QE +L GV D+ +LK ++ + + L DA+ ++ S V++
Sbjct: 1 MAGELISFGIQNLWNLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKN 60
Query: 69 WVSKLKDALYDLDDLIDEFAYEALRRQIMTRNRRNTKQVSIFFSK-SNQVAFRLQMARKI 128
V ++K+ +YD +D I+ F ++ +N T + + + + R + A I
Sbjct: 61 CVEEIKEIIYDGEDTIETF--------VLEQNLGKTSGIKKSIRRLACIIPDRRRYALGI 120
Query: 129 KQVKERLKAINDDKTQFSFSERLID-----TRHDELRARRDIGHYIREEEVIGRSDDKKS 188
+ R+ + D F + ++D + D+ R R + + +G + K
Sbjct: 121 GGLSNRISKVIRDMQSFGVQQAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKK 180
Query: 189 VRKLLVNSSTKDDIAVISIVGMGGLGKTALVQSVYNDKMITKHFELKLWVCVSEEFDIKI 248
+ LV+ + ++ V+SI GMGGLGKT L + V+N + + F+ WVCVS++F
Sbjct: 181 LVGYLVDEA---NVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMN 240
Query: 249 LIEKIIESATGKKPESFLQ---MDSLQDELRKQIDGKKYLLVMDDVWNENDEKWVCLKRL 308
+ +KI+ K+ E + D+LQ EL + ++ K L+V+DD+W + D W +K
Sbjct: 241 VWQKILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIWEKED--WELIKP- 300
Query: 309 LMDGAKGSRILITTRSEQVAKTFETTFL-YSLENLNECNSWLLFKK--MTGLDGQEEELE 368
+ KG ++L+T+R+E VA T+++ + E L +SW LF++ + D E +++
Sbjct: 301 IFPPTKGWKVLLTSRNESVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKID 360
Query: 369 NSNLIEIGKEIVAKLKGVPLAIRTIGRLLKGKKSKRNWLSFKDK-ELHQIKGQEPLEEMQ 428
E+GK ++ G+PLAIR +G +L K + +W + H + G+ +
Sbjct: 361 EEK-EELGKLMIKHCGGLPLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDN 420
Query: 429 S-----ILKLSYEHLQANLKQCFLYCALFPKDYEIQKDDLILQWRAQGFIQSNDKD-ELI 488
+ +L LS+E L + LK CFLY A FP DYEI +L W A+G Q D E+I
Sbjct: 421 NNTCNYVLSLSFEELPSYLKHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEII 480
Query: 489 -DIGEDYFLELLSKSFFQEVTEDELGNIKACKMHDLMHDLACSITKNECVHGRENDIDKR 548
D+G+ Y EL+ ++ + + + C +HD+M ++ K E +
Sbjct: 481 RDVGDVYIEELVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRTST 540
Query: 549 THHFSL-SSKSCEDWKKLTRALSKARN---LRTLFMV--NYSSCWNLDYDKFYHDHLRLR 608
+ S+ +S+ +T + K N LR+L +V Y + +RL
Sbjct: 541 GNSLSIVTSRRLVYQYPITLDVEKDINDPKLRSLVVVANTYMFWGGWSWMLLGSSFIRLE 600
Query: 609 TLQ-LKWFETQQNAKFLESIIGKFKHLRYLNISNLDLKRLPNCITKLYNLEALTLQYLRR 668
L+ L + L S IG+ HLRYLN+ + ++ +P + L L L L L
Sbjct: 601 LLRVLDIHRAKLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVS 660
Query: 669 LETL-PSDAKNWINLRHL----DLYGNENLEFLPDSMTELCKLETLILHGCGKLKELPKD 728
TL P+ K LR+L D+ LE ++ L KLETL +D
Sbjct: 661 GSTLVPNVLKEMQQLRYLALPKDMGRKTKLE-----LSNLVKLETLKNFSTKNCS--LED 720
Query: 729 IKNWINIRYL--DLSWNNSIEFLPDSITQLNNLETLLLSWCSNLKELPKNTKRLINLKHL 788
++ + +R L +L S+E L SI L LE+L + ++L + + I +
Sbjct: 721 LRGMVRLRTLTIELRKETSLETLAASIGGLKYLESLTI---TDLGSEMRTKEAGIVFDFV 780
Query: 789 DMSWCNALTHMPKGLGQLTNLQTINEFVLGENNGGGLSELNELNKLKGRLSIRNLQFCNI 848
+ +MP+ L++ L+ LQ C +
Sbjct: 781 YLKTLTLKLYMPR--------------------------LSKEQHFPSHLTTLYLQHCRL 840
Query: 849 AD--LQMMDKFLQLKSWLQQLWLTWNNPPNELTIGNLDDVYERVLDCLQPHPNLREISIS 908
+ + +++K QLK EL + + ++ P L+++SI
Sbjct: 841 EEDPMPILEKLHQLK---------------ELELRRKSFSGKEMVCSSGGFPQLQKLSIK 900
Query: 909 RYEGVKLCNW-LSSDSLGCLVNIELIHCKRLEHLPRFDQFPHLKYLRFEDLPRIEYIGND 968
E + +W + S+ L +++ C++L+ LP E LP
Sbjct: 901 GLEEWE--DWKVEESSMPVLHTLDIRDCRKLKQLPD------------EHLP-------S 960
Query: 969 NFVSSSTFFPSLKKFIIIGMPKLKRWWKGTTSTESTEPTEGSFPIKFPCLSDLAIYGCP- 1028
+ S S FF L++ MP L+R + + SF + + A G P
Sbjct: 961 HLTSISLFFCCLEE---DPMPTLER----LVHLKELQLLFRSFSGR---IMVCAGSGFPQ 1020
Query: 1029 LHILISWHAPLKSLTIRDSEEVEFGAVPRENFENLSFLLLSRLSKLEYLPECLQHVTTLQ 1088
LH L L L + VE G++P+ L L + R KL+ LP + L+
Sbjct: 1021 LHKL-----KLSELDGLEEWIVEDGSMPQ-----LHTLEIRRCPKLKKLPNGFPQLQNLE 1048
Query: 1089 TLVLSNCENLKSLPEWME------NLTSLTKLEIGYCKKLNSLPEGIRHIHGLQSLRVID 1128
L L EW E ++ L L I C KL LP+G+R I+ L++L V
Sbjct: 1081 ---------LNELEEWEEWIVEDGSMPLLHTLRIWNCPKLKQLPDGLRFIYSLKNLTV-- 1048
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q7XA39 | 1.3e-152 | 33.42 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XA40 | 1.6e-152 | 33.59 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XBQ9 | 2.9e-149 | 33.04 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
| Q7XA42 | 4.2e-148 | 32.66 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q9LRR5 | 1.1e-95 | 28.56 | Putative disease resistance protein At3g14460 OS=Arabidopsis thaliana OX=3702 GN... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DKY9 | 0.0e+00 | 59.42 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
| A0A6J1DHD4 | 0.0e+00 | 53.53 | disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020... | [more] |
| A0A1S4E349 | 0.0e+00 | 57.28 | putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo OX=3... | [more] |
| A0A6J1DPI9 | 0.0e+00 | 52.83 | LOW QUALITY PROTEIN: disease resistance protein RGA2-like OS=Momordica charantia... | [more] |
| A0A6J1FNX2 | 0.0e+00 | 48.84 | putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022154678.1 | 0.0e+00 | 59.42 | putative disease resistance protein RGA4 [Momordica charantia] | [more] |
| XP_022152874.1 | 0.0e+00 | 53.53 | disease resistance protein RGA2-like [Momordica charantia] | [more] |
| XP_016902648.1 | 0.0e+00 | 57.28 | PREDICTED: putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis ... | [more] |
| XP_022154711.1 | 0.0e+00 | 52.83 | LOW QUALITY PROTEIN: disease resistance protein RGA2-like [Momordica charantia] | [more] |
| XP_031736581.1 | 0.0e+00 | 56.48 | putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothe... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G14460.1 | 7.7e-97 | 28.56 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
| AT3G14470.1 | 4.8e-91 | 28.05 | NB-ARC domain-containing disease resistance protein | [more] |
| AT1G58602.1 | 2.7e-73 | 25.98 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
| AT1G58602.2 | 2.7e-73 | 25.98 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
| AT1G58848.1 | 2.0e-65 | 25.36 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
