Lcy10g006540 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy10g006540
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	ATP-dependent DNA helicase
Location	Chr10: 27160251 .. 27161889 (-)
RNA-Seq Expression	Lcy10g006540
Synteny	Lcy10g006540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTGTGCATTTCAAAGACTATTTGCTACTTTGTTGGTATATTATGAACCTACAGAAGTTAGAAAATTATGGAATTGTTTTTTCGAAGCCATTGCAGAAGATTATAAGCGATCAAATGATTTATCCAATGAACAACAAGTTAAATTGGTTTTTAAAAACCTAAATTACTTCCTCCAATCAATGGGAAAAAATATTGCACAATTCGATCTCCCTCCAATTCATACAGACAGCTTCGATGATGACTTCATTTCAATGACAGAGATAAAAGATGAAGAATCCATTACAATAGATGAAGAAGATGTTCTAGCATCAACAAAATTAAATGAGGAACAAAAAGTTGCATATGATATCATCGTGGAAAAAATTGATGGGAATAAAAGTGGACTTTTTTTTTTATCAATGGCCCAGGAGGAACTGGAAAACCCTTTTTGTATCGAACACTTTTAGCAAGAGTTAGATCAAATGGAATGATAGCTTTGGCAACTGCAACATCTGGTGTAGCAGCATCGATATTACCTGGGGGTCGAACAACACATTCTAGATTCAAAATTCCATTACAAGCGACTGATTCGACAATGTGTAATATATCAAAGCAGAGTGTTACAACAGAACTAATTCGAAAAGCAAGCCTAATAATATGGGATGAGGCACCTATGGCAAAAAGATATACGATCGAAACTGTTGATCGATCATTGAGGGATATAATGGATTATACTCAACCATTTGGAGGAAAAGTATTCGTGTTTGGAGGAGATTTTCGTCAAGTACTTCCAGTACTTCCAAAAGGAACAAGACAACAAACTGTTGCAATTAGTTTGAGAAGATCGTATCTTTGGGATAAAATGCATCAACTGCATTTTAAACAGAATATGAGGGTACATAATGACAAAGCATTTGCAGATTTTATCTCAAGAATTGAAAATGTAGAACCTACTTATAATGATGACATTGTTACCTTGTCTCAAGAAATTGCAATACCACATTTTGGAAGTAACATGAAAATGGAATTCATAAAAGAGATATATCCAGATATACAACTTTATGCTACATCAATGGAATACATGACCAATAGAGAAATTTTAGCTACAACAAATGACCATGTTGATTATTTGAATGAACTTTTGATAGATATCTTTCTAGGAGATTATTTGAATGAACTTTTGATAGATATCTTTCTAGGAGAAGTTAAAATCTTTAAAAGCTTCGATGAGGCAATGGATGATACAAATGCCTACTATCAGGAAGAATTTTTAAATTCTTTGCTACCAAATGGAATACCACCACATAAGCTTATATTAAACAAAGCACAAGGACAAACTGTTCCTAATGTTGGAATATACCTTCCTCAACCTGTATTTTCACATGGACAGAACTCTATGTTGCATTATCAAGAGGGGTTTCAATGTCAACAACAAAAGTTCTTATCAAATCACTAAAGGATCCAAATTTGCCTTCCAGACATACAAAAAATGTTGTTTATAAAGAGGTTCTCCTTTAATCAAGGTCCGGTTAATTAAATACTTATTTAATTTTTTTTAAACATCGAGATATAATTCTTATGAAATATAATCTTTTTTTTTTTTTTTTGTCTCCAGAAATCAATCTCTTCCAAGAACCACCAAAGAATTACCCTTGA

mRNA sequence

ATGTCGTGTGCATTTCAAAGACTATTTGCTACTTTGTTGGTATATTATGAACCTACAGAAGTTAGAAAATTATGGAATTGTTTTTTCGAAGCCATTGCAGAAGATTATAAGCGATCAAATGATTTATCCAATGAACAACAAGTTAAATTGGTTTTTAAAAACCTAAATTACTTCCTCCAATCAATGGGAAAAAATATTGCACAATTCGATCTCCCTCCAATTCATACAGACAGCTTCGATGATGACTTCATTTCAATGACAGAGATAAAAGATGAAGAATCCATTACAATAGATGAAGAAGATGTTCTAGCATCAACAAAATTAAATGAGGAACAAAAAGTTGCATATGATATCATCGTGGAAAAAATTGATGGGAATAAAAGAGGAACTGGAAAACCCTTTTTGTATCGAACACTTTTAGCAAGAGTTAGATCAAATGGAATGATAGCTTTGGCAACTGCAACATCTGGTGTAGCAGCATCGATATTACCTGGGGGTCGAACAACACATTCTAGATTCAAAATTCCATTACAAGCGACTGATTCGACAATGTGTAATATATCAAAGCAGAGTGTTACAACAGAACTAATTCGAAAAGCAAGCCTAATAATATGGGATGAGGCACCTATGGCAAAAAGATATACGATCGAAACTGTTGATCGATCATTGAGGGATATAATGGATTATACTCAACCATTTGGAGGAAAAGTATTCGTGTTTGGAGGAGATTTTCGTCAAGTACTTCCAGTACTTCCAAAAGGAACAAGACAACAAACTGTTGCAATTAGTTTGAGAAGATCGTATCTTTGGGATAAAATGCATCAACTGCATTTTAAACAGAATATGAGGGTACATAATGACAAAGCATTTGCAGATTTTATCTCAAGAATTGAAAATGTAGAACCTACTTATAATGATGACATTGTTACCTTGTCTCAAGAAATTGCAATACCACATTTTGGAAAAATCAATCTCTTCCAAGAACCACCAAAGAATTACCCTTGA

Coding sequence (CDS)

ATGTCGTGTGCATTTCAAAGACTATTTGCTACTTTGTTGGTATATTATGAACCTACAGAAGTTAGAAAATTATGGAATTGTTTTTTCGAAGCCATTGCAGAAGATTATAAGCGATCAAATGATTTATCCAATGAACAACAAGTTAAATTGGTTTTTAAAAACCTAAATTACTTCCTCCAATCAATGGGAAAAAATATTGCACAATTCGATCTCCCTCCAATTCATACAGACAGCTTCGATGATGACTTCATTTCAATGACAGAGATAAAAGATGAAGAATCCATTACAATAGATGAAGAAGATGTTCTAGCATCAACAAAATTAAATGAGGAACAAAAAGTTGCATATGATATCATCGTGGAAAAAATTGATGGGAATAAAAGAGGAACTGGAAAACCCTTTTTGTATCGAACACTTTTAGCAAGAGTTAGATCAAATGGAATGATAGCTTTGGCAACTGCAACATCTGGTGTAGCAGCATCGATATTACCTGGGGGTCGAACAACACATTCTAGATTCAAAATTCCATTACAAGCGACTGATTCGACAATGTGTAATATATCAAAGCAGAGTGTTACAACAGAACTAATTCGAAAAGCAAGCCTAATAATATGGGATGAGGCACCTATGGCAAAAAGATATACGATCGAAACTGTTGATCGATCATTGAGGGATATAATGGATTATACTCAACCATTTGGAGGAAAAGTATTCGTGTTTGGAGGAGATTTTCGTCAAGTACTTCCAGTACTTCCAAAAGGAACAAGACAACAAACTGTTGCAATTAGTTTGAGAAGATCGTATCTTTGGGATAAAATGCATCAACTGCATTTTAAACAGAATATGAGGGTACATAATGACAAAGCATTTGCAGATTTTATCTCAAGAATTGAAAATGTAGAACCTACTTATAATGATGACATTGTTACCTTGTCTCAAGAAATTGCAATACCACATTTTGGAAAAATCAATCTCTTCCAAGAACCACCAAAGAATTACCCTTGA

Protein sequence

MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQSMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIVEKIDGNKRGTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHSRFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQPFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFADFISRIENVEPTYNDDIVTLSQEIAIPHFGKINLFQEPPKNYP

Homology

[Close]

BLAST of Lcy10g006540 vs. ExPASy Swiss-Prot
Match: Q6MHJ5 (ATP-dependent DNA helicase pif1 OS=Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100) OX=264462 GN=pif1 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.2e-06
Identity = 50/177 (28.25%), Postives = 74/177 (41.81%), Query Frame = 0


Query: 129 GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHSRFKIPLQ--ATDSTMCN 188
           G+GK FL R  +  +    M  LA   S  AA++L GGRT HS F + +     D+T   
Sbjct: 57  GSGKSFLIRQFMRELDPKEMPILA---STGAAAVLLGGRTFHSFFGLGIMEGGADATYER 116

Query: 189 ISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQPFGGKVFVFGGDFRQ 248
            SK       +RK   +I DE  M     +   +   +   +   P+GG   +  GDF Q
Sbjct: 117 ASKDKRLMSRLRKVEGVIIDEISMIPGQALMIAEALSQRARESKLPWGGMRVIAVGDFAQ 176

Query: 249 VLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFADFISRIENVEPT 304
           + PV   G R       L   +       +    N RV +D  F D +S + + + T
Sbjct: 177 LPPVTHTGQRDWCF---LNGVWEVSGFQTVMLSHNQRV-SDNLFLDVLSDVRHGKVT 226

[Close]

BLAST of Lcy10g006540 vs. ExPASy TrEMBL
Match: A0A1U7XH39 (ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104238658 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 9.7e-88
Identity = 174/331 (52.57%), Postives = 229/331 (69.18%), Query Frame = 0


Query: 1   MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
           M  A + LFAT+LV+  PT++RKLW  ++E +++D+++ +D S   Q++   K++NYFL+
Sbjct: 1   MPSALRNLFATILVHCNPTDIRKLWETYYEDMSKDFRKIHDNSPVAQLQCTLKSINYFLE 60

Query: 61  SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
           SM K I ++DLP +     + +     EI  E SI +   D  A +KLN EQ+ A+  I+
Sbjct: 61  SMDKKIDKYDLPKLDHGRDNGNVSECREIIKERSINVSPADFDAQSKLNPEQEQAFKTIL 120

Query: 121 EKIDGNKR---------GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
           E++D  +          GTGK FLYR LLA +RS GMIALAT TSGVAA+ILPGGRT HS
Sbjct: 121 ERVDSGRAGLFFVDGPGGTGKTFLYRALLANLRSRGMIALATTTSGVAAAILPGGRTAHS 180

Query: 181 RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
           RF IPLQ TD+T+ N+SKQS   +LIRK+ LIIWDEAPMA R TIETVDRS RDIMD  +
Sbjct: 181 RFDIPLQTTDTTITNMSKQSGGAKLIRKSKLIIWDEAPMANRQTIETVDRSFRDIMDINE 240

Query: 241 PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
           PFGGKV VFGGDFRQVLPV+PK TR +T+  SL +SYLW+KM ++   +NMR   D  F+
Sbjct: 241 PFGGKVMVFGGDFRQVLPVVPKSTRVETINASLVKSYLWNKMEKIQLIRNMRARADPTFS 300

Query: 301 DFISRIEN-VEPTYNDDIVTLSQEIAIPHFG 322
           DF+ RI N  EPT  DD+V L +++ + H G
Sbjct: 301 DFLLRIGNGEEPTIRDDMVLLLEKLIVKHDG 331

[Close]

BLAST of Lcy10g006540 vs. ExPASy TrEMBL
Match: A0A1S4CBN9 (ATP-dependent DNA helicase OS=Nicotiana tabacum OX=4097 GN=LOC107817267 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 6.3e-87
Identity = 173/329 (52.58%), Postives = 225/329 (68.39%), Query Frame = 0


Query: 1   MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
           M  A + LFAT+LV+    +VRK+W+ ++E ++ED++R +D S   Q++   K++ YFL+
Sbjct: 1   MPLALRNLFATILVHCNSMDVRKMWDTYYEDMSEDFRRIHDNSPATQLQCTLKSIKYFLE 60

Query: 61  SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
           SM K I  FD+P  + D  ++      EI +E S  I  ED  A +KLN EQ+ A+  I+
Sbjct: 61  SMSKRIQNFDIPAANQDLDNNSLSECREIIEERSTKIPAEDFDAQSKLNAEQEQAFKTIL 120

Query: 121 EKIDG---------NKRGTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
           +++D             GTGK FLYR LL  VRS  MIALAT TSGVA +ILPGGRT HS
Sbjct: 121 QRVDSGIVGLFFVDGPGGTGKTFLYRALLVNVRSRSMIALATTTSGVAVAILPGGRTAHS 180

Query: 181 RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
           +F IPLQ TD+T+ N+SKQS + +LIRKA LIIWDEAPMAKR TIETVDRS RDIMD  +
Sbjct: 181 KFDIPLQTTDTTITNMSKQSGSAKLIRKAKLIIWDEAPMAKRQTIETVDRSFRDIMDNDE 240

Query: 241 PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
           PFGGKV VFGGDFRQVLPV+PK TR +T+  SL +SYLW KM ++   +NMR   D AF+
Sbjct: 241 PFGGKVMVFGGDFRQVLPVVPKSTRAETINASLVKSYLWHKMEKIKLTKNMRARTDPAFS 300

Query: 301 DFISRI-ENVEPTYNDDIVTLSQEIAIPH 320
           DF+ RI +  EPT  D++V L +EI + H
Sbjct: 301 DFLLRIGDGEEPTIRDNLVQLPEEIVVKH 329

[Close]

BLAST of Lcy10g006540 vs. ExPASy TrEMBL
Match: A0A5B7A9C3 (ATP-dependent DNA helicase OS=Davidia involucrata OX=16924 GN=Din_021512 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 1.4e-86
Identity = 170/329 (51.67%), Postives = 231/329 (70.21%), Query Frame = 0


Query: 1    MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
            M  A +RLFAT+LVY EPT+VRKLW+ +FEA++ED+KR++  S E Q+    K+L +FL+
Sbjct: 923  MPHALRRLFATILVYCEPTDVRKLWDTYFEAMSEDFKRASGSSIESQICKTIKSLEFFLE 982

Query: 61   SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
            SMGK+I  +DLP    D  D       EI+DE ++ I  +D  A   LN EQ+ A+ +I+
Sbjct: 983  SMGKSIRNYDLPDTPLDPLDARESYPREIRDEMAVDIPPDDYSAELNLNPEQQNAFSLIL 1042

Query: 121  EKIDGNKR---------GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
            ++++  K          GTGK +LY  LLA +RS  MIALATATSGVAA+I+PGGRT HS
Sbjct: 1043 DRVESEKSGIFFIDGPGGTGKTYLYCALLANLRSRKMIALATATSGVAAAIMPGGRTAHS 1102

Query: 181  RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
            RFKIP+   ++++CNI KQS T EL+RKA LIIWDE PMAKR+ IET+DR+LRDIM+  +
Sbjct: 1103 RFKIPISVNETSICNIPKQSGTAELLRKARLIIWDETPMAKRWAIETLDRTLRDIMNNQE 1162

Query: 241  PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
             FGGKV VFGGDFRQVLPV+P+ TR++TV  SL +S++W KM +L   +NMR   D  F+
Sbjct: 1163 SFGGKVIVFGGDFRQVLPVVPRATREETVNASLVKSHIWSKMEKLTLTRNMRARADPNFS 1222

Query: 301  DFISRIEN-VEPTYNDDIVTLSQEIAIPH 320
            DF+ R+ N  EPT N D++ +  ++ I +
Sbjct: 1223 DFLLRVGNGEEPTNNQDMILIPDQMVIKY 1251

[Close]

BLAST of Lcy10g006540 vs. ExPASy TrEMBL
Match: A0A1U7V7Y5 (ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104213305 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 3.3e-80
Identity = 165/311 (53.05%), Postives = 215/311 (69.13%), Query Frame = 0


Query: 32  IAEDYKRSNDLSNEQQVKLVFKNLNYFLQSMGKNIAQFDLPPIHTDSFDDDFISMTEIKD 91
           ++ED+++  D S   Q++   K++NYFL+SMGK I ++DLP +     + +     EI +
Sbjct: 1   MSEDFRKIRDNSPVAQLQCTLKSINYFLESMGKKIDKYDLPKLDHGRDNGNVSECREIIE 60

Query: 92  EESITIDEEDVLASTKLNEEQKVAYDIIVEKIDGNKR---------GTGKPFLYRTLLAR 151
           E SI +  ED  A +KLN EQ+ A+  I+E++D  +          GTGK FLYR LLA 
Sbjct: 61  ERSINVSPEDFDAQSKLNPEQEQAFKTILERVDSGRAGLFFVDGPGGTGKTFLYRALLAN 120

Query: 152 VRSNGMIALATATSGVAASILPGGRTTHSRFKIPLQATDSTMCNISKQSVTTELIRKASL 211
           +RS GMIALATATSGVAA+ILPGGRTTHSRF IPLQ TD+T+ N+SKQS   +LIRK+ L
Sbjct: 121 LRSRGMIALATATSGVAAAILPGGRTTHSRFDIPLQTTDTTITNMSKQSGGAKLIRKSKL 180

Query: 212 IIWDEAPMAKRYTIETVDRSLRDIMDYTQPFGGKVFVFGGDFRQVLPVLPKGTRQQTVAI 271
           IIWDEAPMA R TIETVDRS RDIMD  +PFGGKV VFGGDFRQVLPV+PK TR +T+  
Sbjct: 181 IIWDEAPMANRQTIETVDRSFRDIMDINEPFGGKVMVFGGDFRQVLPVVPKSTRVETINA 240

Query: 272 SLRRSYLWDKMHQLHFKQNMRVHNDKAFADFISRIEN-VEPTYNDDIVTLSQEIAIPH-- 329
           SL +SYLW+KM ++   +NMR   D  F+DF+ RI N  EPT   D++ L +++ + H  
Sbjct: 241 SLVKSYLWNKMEKIQLIRNMRARADPTFSDFLLRIGNGEEPTIRYDMILLLEKLIVKHDG 300

[Close]

BLAST of Lcy10g006540 vs. ExPASy TrEMBL
Match: A0A1D6NYF5 (ATP-dependent DNA helicase OS=Zea mays OX=4577 GN=103633322 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 6.3e-79
Identity = 172/336 (51.19%), Postives = 221/336 (65.77%), Query Frame = 0


Query: 1    MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYK-RSNDLSNEQQVKLVFKNLNYFL 60
            M  A +RLFAT+LVY EP++V  LW    + ++EDY+ RS   ++ +Q+ L+  ++   L
Sbjct: 1240 MPSALRRLFATILVYCEPSDVAVLWQNHLDVMSEDYQHRSQGKTHVEQMVLI--DIRNML 1299

Query: 61   QSMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDII 120
            QSMGK+I  F LPPI  D++DD   +  E+ +EESI     DV     LNEEQ+VAYD I
Sbjct: 1300 QSMGKDIKTFPLPPI-IDAYDDAIGTAREVYEEESIEPAVGDVALKDSLNEEQRVAYDKI 1359

Query: 121  VEKIDGNK---------RGTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTH 180
            +  +D +K          GTGK +LYR LLA +RS G IA+ATATSGVAASI+PGGRT H
Sbjct: 1360 LSAVDTDKGGLFFVDGPGGTGKTYLYRVLLATLRSQGKIAVATATSGVAASIMPGGRTAH 1419

Query: 181  SRFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYT 240
            SRFKIPL   D  +C+ +KQS T EL+RKASLIIWDEA M KR  +E +D S+RDIM   
Sbjct: 1420 SRFKIPLTIDDGAVCSFTKQSGTAELLRKASLIIWDEASMTKRQAVEALDNSMRDIMGRP 1479

Query: 241  Q-PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKA 300
            + PFGGK  VFGGDFRQVLPV+ KG+R Q VA SLR SYLW+ M  L    NMR  ND  
Sbjct: 1480 ELPFGGKTIVFGGDFRQVLPVVRKGSRAQVVASSLRMSYLWESMSHLKLVSNMRAKNDPW 1539

Query: 301  FADFISRI-ENVEPTYNDDIVTLSQEIAIPHFGKIN 325
            FA+++ R+    E T +D  + L  E+ +P+ G  N
Sbjct: 1540 FAEYLLRVGGGTEDTNSDGDICLPDEVCVPYSGSDN 1572

[Close]

BLAST of Lcy10g006540 vs. NCBI nr
Match: XP_019228080.1 (PREDICTED: ATP-dependent DNA helicase PIF1-like [Nicotiana attenuata])

HSP 1 Score: 349.0 bits (894), Expect = 4.6e-92
Identity = 182/342 (53.22%), Postives = 240/342 (70.18%), Query Frame = 0


Query: 1   MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
           M  A + LFAT+LV+  PT++RKLW  ++E ++ED+++ +D S   Q++   K++NYFL+
Sbjct: 132 MPSALRNLFATILVHCNPTDIRKLWETYYEDMSEDFRKIHDNSPAAQLQCTLKSINYFLE 191

Query: 61  SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
           SMGK I ++DLP +     + +     EI +E SI +  ED  A +KLN EQ+ A+  I+
Sbjct: 192 SMGKKIGKYDLPKLDHGRDNGNVSECREIIEERSINVSPEDFDAQSKLNSEQEQAFKTIL 251

Query: 121 EKIDGNKR---------GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
           E++D  +          GTGK FLYR LLA +RS GMIALATATSGVAA+ILPGGRTTHS
Sbjct: 252 ERVDSGRAGLFFVDGPGGTGKTFLYRALLANLRSRGMIALATATSGVAAAILPGGRTTHS 311

Query: 181 RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
           RF IPLQ TD+T+ N+SKQS   +LIRK+ LIIWDEAPMA R TIETVDRS+RDIMD  +
Sbjct: 312 RFDIPLQTTDTTITNMSKQSGGAKLIRKSKLIIWDEAPMANRQTIETVDRSIRDIMDINE 371

Query: 241 PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
           PFGGKV VFGGDFRQVLPV+PK TR +T+  SL +SYLW+KM ++   +NMR   D  F+
Sbjct: 372 PFGGKVMVFGGDFRQVLPVVPKSTRAETINASLVKSYLWNKMEKIQLTRNMRARADPTFS 431

Query: 301 DFISRIEN-VEPTYNDDIVTLSQEIAIPH----FGKINLFQE 329
           DF+ RI N  EPT  DD+V L +++ + H     G+ NL +E
Sbjct: 432 DFLLRIGNGEEPTIRDDMVLLPEKLIVKHDADSTGEDNLIRE 473

[Close]

BLAST of Lcy10g006540 vs. NCBI nr
Match: XP_019242534.1 (PREDICTED: uncharacterized protein LOC109222664, partial [Nicotiana attenuata])

HSP 1 Score: 345.5 bits (885), Expect = 5.1e-91
Identity = 178/329 (54.10%), Postives = 232/329 (70.52%), Query Frame = 0


Query: 1   MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
           M  A + LFAT+LV+  P +VRKLW+ ++E ++ED++R +D S   Q++   K++ YFL+
Sbjct: 411 MPLALRNLFATILVHCNPMDVRKLWDTYYEDMSEDFRRIHDNSPATQLQCTLKSIKYFLE 470

Query: 61  SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
           SMGK I  +D+P  + D  ++      EI +E SI +  ED  A +KLN EQ+ A+  I+
Sbjct: 471 SMGKRIQNYDIPAANQDLDNNSPSECREIIEERSIKVPAEDFDAQSKLNAEQEQAFKTIL 530

Query: 121 EKIDGNKR---------GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
           +++D  +          GTGK FLYR LLA VRS GM+ALAT TSGVAA+ILPGGRT HS
Sbjct: 531 QRVDSGRAGLFFVDGPGGTGKTFLYRALLANVRSRGMVALATTTSGVAAAILPGGRTAHS 590

Query: 181 RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
           RF IPLQ TD+T+ N+SKQS + +LIRKA LIIWDEAPMAKR TIETVDRS RDIMD  +
Sbjct: 591 RFDIPLQTTDTTITNMSKQSGSAKLIRKAKLIIWDEAPMAKRQTIETVDRSFRDIMDNNE 650

Query: 241 PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
           PFGGKV +FGGDFRQVLPV+PK TR +T+  SL +SYLW KM ++   +NMR   D AF+
Sbjct: 651 PFGGKVMIFGGDFRQVLPVVPKSTRAETINTSLVKSYLWHKMEKIKLTRNMRARTDPAFS 710

Query: 301 DFISRI-ENVEPTYNDDIVTLSQEIAIPH 320
           DF+ RI +  EPT  DD+V L +EI + H
Sbjct: 711 DFLLRIGDGEEPTIRDDLVQLPEEIVVKH 739

[Close]

BLAST of Lcy10g006540 vs. NCBI nr
Match: KAG5531013.1 (hypothetical protein RHGRI_025837 [Rhododendron griersonianum])

HSP 1 Score: 339.3 bits (869), Expect = 3.6e-89
Identity = 173/332 (52.11%), Postives = 236/332 (71.08%), Query Frame = 0


Query: 1    MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
            M  A + LFA +LVY +P++VRKLW  ++E ++ED+ R   ++ E ++    K+++ FL+
Sbjct: 927  MPQALRNLFAVILVYCDPSDVRKLWQDYYEPMSEDFLRLEGVNEELRLLKTLKSIDMFLE 986

Query: 61   SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
            S+GK+I  +DLP I +   +   +   E+ DE ++    ED LA  KLN EQK AY+II+
Sbjct: 987  SIGKSIKDYDLPAIKSQIVEIGIVEKREVLDELAVKNPPEDCLAPAKLNPEQKYAYNIIL 1046

Query: 121  EKIDGNKR---------GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
            + ++ NK          G+GK +LYR LLA +RSNGMIALATATSGVAASILPGGRT HS
Sbjct: 1047 DHVNNNKNGSFFIDGPGGSGKTYLYRALLATIRSNGMIALATATSGVAASILPGGRTAHS 1106

Query: 181  RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
            RFKIP+   DS++CNI KQS T ELIR+A LIIWDEAPMAKR+ IE +DRSL+DIM    
Sbjct: 1107 RFKIPIDGNDSSVCNIPKQSGTAELIRRAKLIIWDEAPMAKRWAIEALDRSLKDIMSNNS 1166

Query: 241  PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
             FGGKV VFGGDFRQVLPV+P+GTR +TV  SL +SYLW +M +L  K NMR  +D AF+
Sbjct: 1167 LFGGKVIVFGGDFRQVLPVVPRGTRAETVHASLAKSYLWTEMQKLILKTNMRAQSDSAFS 1226

Query: 301  DFISRIEN-VEPTYNDDIVTLSQEIAIPHFGK 323
            DF+ R+ N VEPT +++++ + +E+ + + G+
Sbjct: 1227 DFLLRVGNGVEPTVDENMIQIPEEMIVRYEGR 1258

[Close]

BLAST of Lcy10g006540 vs. NCBI nr
Match: KAG5520322.1 (hypothetical protein RHGRI_033033 [Rhododendron griersonianum])

HSP 1 Score: 337.0 bits (863), Expect = 1.8e-88
Identity = 175/325 (53.85%), Postives = 230/325 (70.77%), Query Frame = 0


Query: 1    MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
            M  A ++LFAT+LVY EP++VRKLW+  FEA++ED+ R   +S E  +    ++++YFL 
Sbjct: 901  MPLALRQLFATVLVYCEPSDVRKLWDLLFEAMSEDFTRKEAVSTEVCLLKTLRSVDYFLD 960

Query: 61   SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
             MG++I  +DLP + ++  + +FI   E+ DE  I I  ED+ A T LN EQK AYDII+
Sbjct: 961  GMGRSIKDYDLPSMMSNICETEFIGFREVLDELQIRIPPEDISALTSLNLEQKSAYDIIL 1020

Query: 121  EKIDGNKR---------GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
            +++  N           GTGK  LYR LLA +RS GMIA+ATATSGVAASI+PGGRT+HS
Sbjct: 1021 DQVSSNVNGLFFIDGPGGTGKTHLYRALLATIRSKGMIAIATATSGVAASIMPGGRTSHS 1080

Query: 181  RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
            RFKIP+   +S++CNI KQS T EL+R A LIIWDEAPMAKR+ IE +DRSLRDIM    
Sbjct: 1081 RFKIPIDGNESSVCNIPKQSGTAELLRLAKLIIWDEAPMAKRWAIEALDRSLRDIMSNDV 1140

Query: 241  PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
             FGGKV VFGGDFRQVLPV+P+GTR +TV  SL  SYLW KM +L   +NMR  +D  F+
Sbjct: 1141 LFGGKVIVFGGDFRQVLPVVPRGTRVETVNASLVMSYLWPKMQKLKLSKNMRAQHDSMFS 1200

Query: 301  DFISRIEN-VEPTYNDDIVTLSQEI 316
            DF+ R+ N  EPT N+D++ + +E+
Sbjct: 1201 DFLLRVGNGDEPTINEDLIQIPKEM 1225

[Close]

BLAST of Lcy10g006540 vs. NCBI nr
Match: KAG5520323.1 (hypothetical protein RHGRI_033033 [Rhododendron griersonianum])

HSP 1 Score: 337.0 bits (863), Expect = 1.8e-88
Identity = 175/325 (53.85%), Postives = 230/325 (70.77%), Query Frame = 0


Query: 1    MSCAFQRLFATLLVYYEPTEVRKLWNCFFEAIAEDYKRSNDLSNEQQVKLVFKNLNYFLQ 60
            M  A ++LFAT+LVY EP++VRKLW+  FEA++ED+ R   +S E  +    ++++YFL 
Sbjct: 901  MPLALRQLFATVLVYCEPSDVRKLWDLLFEAMSEDFTRKEAVSTEVCLLKTLRSVDYFLD 960

Query: 61   SMGKNIAQFDLPPIHTDSFDDDFISMTEIKDEESITIDEEDVLASTKLNEEQKVAYDIIV 120
             MG++I  +DLP + ++  + +FI   E+ DE  I I  ED+ A T LN EQK AYDII+
Sbjct: 961  GMGRSIKDYDLPSMMSNICETEFIGFREVLDELQIRIPPEDISALTSLNLEQKSAYDIIL 1020

Query: 121  EKIDGNKR---------GTGKPFLYRTLLARVRSNGMIALATATSGVAASILPGGRTTHS 180
            +++  N           GTGK  LYR LLA +RS GMIA+ATATSGVAASI+PGGRT+HS
Sbjct: 1021 DQVSSNVNGLFFIDGPGGTGKTHLYRALLATIRSKGMIAIATATSGVAASIMPGGRTSHS 1080

Query: 181  RFKIPLQATDSTMCNISKQSVTTELIRKASLIIWDEAPMAKRYTIETVDRSLRDIMDYTQ 240
            RFKIP+   +S++CNI KQS T EL+R A LIIWDEAPMAKR+ IE +DRSLRDIM    
Sbjct: 1081 RFKIPIDGNESSVCNIPKQSGTAELLRLAKLIIWDEAPMAKRWAIEALDRSLRDIMSNDV 1140

Query: 241  PFGGKVFVFGGDFRQVLPVLPKGTRQQTVAISLRRSYLWDKMHQLHFKQNMRVHNDKAFA 300
             FGGKV VFGGDFRQVLPV+P+GTR +TV  SL  SYLW KM +L   +NMR  +D  F+
Sbjct: 1141 LFGGKVIVFGGDFRQVLPVVPRGTRVETVNASLVMSYLWPKMQKLKLSKNMRAQHDSMFS 1200

Query: 301  DFISRIEN-VEPTYNDDIVTLSQEI 316
            DF+ R+ N  EPT N+D++ + +E+
Sbjct: 1201 DFLLRVGNGDEPTINEDLIQIPKEM 1225

The following BLAST results are available for this feature:

BLAST of Lcy10g006540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6MHJ5	5.2e-06	28.25	ATP-dependent DNA helicase pif1 OS=Bdellovibrio bacteriovorus (strain ATCC 15356...	[more]

BLAST of Lcy10g006540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1U7XH39	9.7e-88	52.57	ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104238658 PE=3 ...	[more]
A0A1S4CBN9	6.3e-87	52.58	ATP-dependent DNA helicase OS=Nicotiana tabacum OX=4097 GN=LOC107817267 PE=3 SV=...	[more]
A0A5B7A9C3	1.4e-86	51.67	ATP-dependent DNA helicase OS=Davidia involucrata OX=16924 GN=Din_021512 PE=3 SV...	[more]
A0A1U7V7Y5	3.3e-80	53.05	ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104213305 PE=3 ...	[more]
A0A1D6NYF5	6.3e-79	51.19	ATP-dependent DNA helicase OS=Zea mays OX=4577 GN=103633322 PE=3 SV=1	[more]

BLAST of Lcy10g006540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_019228080.1	4.6e-92	53.22	PREDICTED: ATP-dependent DNA helicase PIF1-like [Nicotiana attenuata]	[more]
XP_019242534.1	5.1e-91	54.10	PREDICTED: uncharacterized protein LOC109222664, partial [Nicotiana attenuata]	[more]
KAG5531013.1	3.6e-89	52.11	hypothetical protein RHGRI_025837 [Rhododendron griersonianum]	[more]
KAG5520322.1	1.8e-88	53.85	hypothetical protein RHGRI_033033 [Rhododendron griersonianum]	[more]
KAG5520323.1	1.8e-88	53.85	hypothetical protein RHGRI_033033 [Rhododendron griersonianum]	[more]

BLAST of Lcy10g006540 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010285	DNA helicase Pif1-like	PFAM	PF05970	PIF1	coord: 107..317 e-value: 5.5E-65 score: 219.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 105..273 e-value: 4.5E-22 score: 80.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 32..290
None	No IPR available	PANTHER	PTHR10492	UNCHARACTERIZED	coord: 1..322
None	No IPR available	PANTHER	PTHR10492:SF76	ATP-DEPENDENT DNA HELICASE	coord: 1..322

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy10g006540.1	Lcy10g006540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0000723	telomere maintenance
molecular_function	GO:0003678	DNA helicase activity