Lcy10g006230 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g006230
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPolynucleotidyl transferase, ribonuclease H-like superfamily protein
LocationChr10: 26102644 .. 26103759 (-)
RNA-Seq ExpressionLcy10g006230
SyntenyLcy10g006230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAACTCCTCCTTCATGTTCATCAATCCTTTATGTGCAGAAGCTGTTGCTGTTTTGGAGGGGCTTCAATTGGCTCATAGAATGAATATGAGACGAATTAAGATATTATCTGATTCATTTACTCTTGTCTCAATTCTGCATAAGGAGGTGGAATGTGATGCTAACATTGCTCCAGTCATTTGGGATATAGAGCAAACTAGAAATATGTTTGAGAGTGTTAAAATTTTTCATGTGAGTCATCAATATAATGTGATGACTCATAAATTGGCTCGTTCAGGTTTGTTTTTTGTTCCAAATTTGTGGATTACCAACTATCCAGATTGGATTTCTAAGTTGGTTGTTAGTGAGTTAATGAAACTTTTTTCCATTGTCTAAAAAAACCATTTTTTTCATCAAACAAAAGTTGGGTAGCCGTCGGTTTCATTCAACATGTTTTTCTACTGTAGTCCCAAAAAAAAAGAAAAAAAAAACATGTTTTTCTACCGTGTGAAAAATTAAGCAGAGAGAAAAAAAAAAAAGACAAAATGGTAATTTCACCCACTCAAAAGTCGAAAAAGAAAAACCTTTGCACTCAAAAGAAAAAGAAAAAAAAAAAAAAAAAAAAACCTCCTGAATCTCTTTAGCCCTCCTCTTATAGTCCCGTATTTGGCAAATTCCAATGAACATTACTCTCAGTTTTGAAATCAGATGATTTCGCCACCTCCATGAAGAAGAACCAGGTCTGCAACCTGCGAACCATTACTGGACCTTCCGATTCCATCATACTCTAAAACCCATTAGCTTTTCTCTGTTTTCTTCAACAACGGAAAAGAAGGAACAGTGGTAGGTTTCCTTTCAGTTCTGCCCTCACTCCTTCCTCAGCTTTTCTATGCAATTTGGGACAAATCCATTCTCTGGTTTCCACGTCGCCGCTTTATTGGATGGCGATATCTAGTTTGAGCGCGCAAAAATTGGTTTATTGTTATCACATTTTGCTGTGTTTCTATCTTTCGCCGTGCCCTTTTGTCTGTTGCAGAAAATTTTCGTTCTTTAACTTTAGGAGTGCAGGTGGGACTTGGAAGAAGTTCAAATTGGTTGCTCGATTTCGTTCGGGAGTACGGTTCCGGGAGTTGA

mRNA sequence

ATGGTTAACTCCTCCTTCATGTTCATCAATCCTTTATGTGCAGAAGCTGTTGCTGTTTTGGAGGGGCTTCAATTGGCTCATAGAATGAATATGAGACGAATTAAGATATTATCTGATTCATTTACTCTTGTCTCAATTCTGCATAAGGAGGTGGAATGTGATGCTAACATTGCTCCAGTCATTTGGGATATAGAGCAAACTAGAAATATGTTTGAGAGTGTTAAAATTTTTCATGTGAGTCATCAATATAATGTGATGACTCATAAATTGGCTCGTTCAGGAGTGCAGGTGGGACTTGGAAGAAGTTCAAATTGGTTGCTCGATTTCGTTCGGGAGTACGGTTCCGGGAGTTGA

Coding sequence (CDS)

ATGGTTAACTCCTCCTTCATGTTCATCAATCCTTTATGTGCAGAAGCTGTTGCTGTTTTGGAGGGGCTTCAATTGGCTCATAGAATGAATATGAGACGAATTAAGATATTATCTGATTCATTTACTCTTGTCTCAATTCTGCATAAGGAGGTGGAATGTGATGCTAACATTGCTCCAGTCATTTGGGATATAGAGCAAACTAGAAATATGTTTGAGAGTGTTAAAATTTTTCATGTGAGTCATCAATATAATGTGATGACTCATAAATTGGCTCGTTCAGGAGTGCAGGTGGGACTTGGAAGAAGTTCAAATTGGTTGCTCGATTTCGTTCGGGAGTACGGTTCCGGGAGTTGA

Protein sequence

MVNSSFMFINPLCAEAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFESVKIFHVSHQYNVMTHKLARSGVQVGLGRSSNWLLDFVREYGSGS
Homology
BLAST of Lcy10g006230 vs. ExPASy TrEMBL
Match: A0A6P6UBL0 (uncharacterized protein LOC113709083 OS=Coffea arabica OX=13443 GN=LOC113709083 PE=4 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 8.9e-04
Identity = 31/107 (28.97%), Postives = 57/107 (53.27%), Query Frame = 0

Query: 11  PLCAEAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNM 70
           P+  EA+A+   + +A ++   +IKI SDS +++  +H+  E D NIA ++ D++     
Sbjct: 31  PMKEEALAIRNAMLMAKQVGWTKIKIHSDSKSVLDQIHRGNEYDVNIATILEDVKDLTTH 90

Query: 71  FESVKIFHVSHQYNVMTHKLARSGVQV--GLGRSSN---WLLDFVRE 113
           FE      +    N ++H LA+  VQ+   +    +   WL+D VR+
Sbjct: 91  FERCSFVFIPRTENEISHALAKFAVQLVDDIQWEQDFPVWLMDLVRK 137

BLAST of Lcy10g006230 vs. ExPASy TrEMBL
Match: A0A2N9I609 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS47740 PE=4 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 8.9e-04
Identity = 26/82 (31.71%), Postives = 45/82 (54.88%), Query Frame = 0

Query: 14   AEAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFES 73
            AEA+AV E +QLA  + + ++++  DS  +   L ++  C A+   +I D+ Q  +  + 
Sbjct: 1233 AEAIAVHEAVQLARDVGLTKVEVEGDSLVIFQALEQQDVCFASYGDIIQDVHQVASSLDR 1292

Query: 74   VKIFHVSHQYNVMTHKLARSGV 96
            V   HV    N + H LAR+G+
Sbjct: 1293 VGFSHVCRHSNRVAHVLARNGL 1314

BLAST of Lcy10g006230 vs. ExPASy TrEMBL
Match: A0A2N9EI79 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2337 PE=4 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 8.9e-04
Identity = 31/94 (32.98%), Postives = 51/94 (54.26%), Query Frame = 0

Query: 14   AEAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFES 73
            AEA+AV E +QLA  + +  +++  DS  ++  L ++  C A+   +I DI Q     + 
Sbjct: 943  AEAIAVREAVQLARDVGLAEVEVEGDSLVILHALKQQEVCFASYGDIIQDIHQLACSLDR 1002

Query: 74   VKIFHVSHQYNVMTHKLARSGVQVGLGRSSNWLL 108
            V   HV    N +TH LAR+ +    G SS++L+
Sbjct: 1003 VVFSHVRRTENRVTHVLARNAI----GLSSDFLV 1032

BLAST of Lcy10g006230 vs. ExPASy TrEMBL
Match: A0A2N9EM29 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS3764 PE=4 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 8.9e-04
Identity = 31/94 (32.98%), Postives = 51/94 (54.26%), Query Frame = 0

Query: 14  AEAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFES 73
           AEA+AV E +QLA  + +  +++  DS  ++  L ++  C A+   +I DI Q     + 
Sbjct: 546 AEAIAVREAVQLARDVGLAEVEVEGDSLVILHALKQQEVCFASYGDIIQDIHQLACSLDR 605

Query: 74  VKIFHVSHQYNVMTHKLARSGVQVGLGRSSNWLL 108
           V   HV    N +TH LAR+ +    G SS++L+
Sbjct: 606 VVFSHVRRTENRVTHVLARNAI----GLSSDFLV 635

BLAST of Lcy10g006230 vs. NCBI nr
Match: XP_017250619.1 (PREDICTED: uncharacterized protein LOC108221234 [Daucus carota subsp. sativus])

HSP 1 Score: 60.1 bits (144), Expect = 1.5e-05
Identity = 38/104 (36.54%), Postives = 53/104 (50.96%), Query Frame = 0

Query: 9    INPLCAEAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTR 68
            ++ L AEA+A++ GLQ A        K+L+DS +LV  L+ E E    +  +I D  +  
Sbjct: 1459 LSVLHAEALALVVGLQWAQTTGYTLKKVLTDSQSLVQALNSEAEYHNELGLLIADARELM 1518

Query: 69   NMFESVKIFHVSHQYNVMTHKLARSGVQVGLGRSSNWLLDFVRE 113
              F  VK  HVS   NV  H LAR  +Q  L    +WL D + E
Sbjct: 1519 QYFPGVKSSHVSRNLNVQAHNLARQALQ--LDEEVSWLEDGLNE 1560

BLAST of Lcy10g006230 vs. NCBI nr
Match: XP_042974784.1 (uncharacterized protein LOC122306423 [Carya illinoinensis])

HSP 1 Score: 55.8 bits (133), Expect = 2.8e-04
Identity = 32/94 (34.04%), Postives = 50/94 (53.19%), Query Frame = 0

Query: 15   EAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFESV 74
            EAVA+L GLQL  +  + ++ + +D   LV+ L+   EC  + A ++ DI +    F+ V
Sbjct: 1043 EAVALLRGLQLCVQWGIPKLMLETDCLILVNALNGNSECLTDFAFILQDIRRLMAAFQEV 1102

Query: 75   KIFHVSHQYNVMTHKLARSGVQVGLGRSSNWLLD 109
            K+ HV+H  N + H LAR            WL+D
Sbjct: 1103 KVVHVNHLGNQVAHLLARHA----------WLID 1126

BLAST of Lcy10g006230 vs. NCBI nr
Match: XP_021722392.1 (uncharacterized protein LOC110689869 [Chenopodium quinoa])

HSP 1 Score: 54.3 bits (129), Expect = 8.2e-04
Identity = 31/112 (27.68%), Postives = 61/112 (54.46%), Query Frame = 0

Query: 3   NSSFMFINPLCAEAVAVLEGLQLAHRMNMRRIKILSDSFTLVSIL-------HKEVECDA 62
           ++ +  +NPL  E  A+++GL +A  + +++I++ +D+ +L ++L       H E     
Sbjct: 32  SAKYNAVNPLATELYAIIDGLAIAQDLGVKKIEVETDAESLRTMLLTIGGNPHHE----- 91

Query: 63  NIAPVIWDIEQTRNMFESVKIFHVSHQYNVMTHKLARSGVQVGLGRSSNWLL 108
            +A VI D     + FE   I H+  + N+M HKLA  G  + +G  +++L+
Sbjct: 92  -LAAVICDAASLFSDFEVAFINHIPREMNIMVHKLAEYGKDMAVGYKTHYLV 137

BLAST of Lcy10g006230 vs. NCBI nr
Match: XP_042962672.1 (uncharacterized protein LOC122296942 [Carya illinoinensis])

HSP 1 Score: 54.3 bits (129), Expect = 8.2e-04
Identity = 31/94 (32.98%), Postives = 52/94 (55.32%), Query Frame = 0

Query: 15   EAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFESV 74
            EAVA+L GLQL  +  + +I + +D   LV+ L++   C  +IA ++ DI +    F+ V
Sbjct: 1228 EAVALLRGLQLCAQWGVPKIMLETDCLVLVNALNENSVCLTDIAFILQDIRRLMVGFQEV 1287

Query: 75   KIFHVSHQYNVMTHKLARSGVQVGLGRSSNWLLD 109
            ++ HV+   N++ H+LAR            WL+D
Sbjct: 1288 QVVHVNRLGNLVAHRLARHA----------WLID 1311

BLAST of Lcy10g006230 vs. NCBI nr
Match: XP_042969199.1 (uncharacterized protein LOC122301911 [Carya illinoinensis])

HSP 1 Score: 54.3 bits (129), Expect = 8.2e-04
Identity = 27/78 (34.62%), Postives = 47/78 (60.26%), Query Frame = 0

Query: 15  EAVAVLEGLQLAHRMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFESV 74
           EA+A+L GLQL  +  + ++ + +D   LV+ L++  EC  + A ++ DI +    F+ V
Sbjct: 265 EAIALLRGLQLCVQWGVPKLMLKTDCLILVNALNENSECLTDFAFILQDIRRLMAAFQEV 324

Query: 75  KIFHVSHQYNVMTHKLAR 93
           K+ HV+   N++ H LAR
Sbjct: 325 KVVHVNRLGNLVAHLLAR 342

BLAST of Lcy10g006230 vs. TAIR 10
Match: AT5G42965.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 1.8e-04
Identity = 23/70 (32.86%), Postives = 38/70 (54.29%), Query Frame = 0

Query: 28  RMNMRRIKILSDSFTLVSILHKEVECDANIAPVIWDIEQTRNMFESVKIFHVSHQYNVMT 87
           R N +++   SDS  LVS+L  + +   N+AP I DI      FE VK+ H+  + N + 
Sbjct: 32  RFNYKKVIFESDSQHLVSLLGDDTDY-PNLAPRIQDIRHIFQQFEEVKVMHIKREGNSVA 91

Query: 88  HKLARSGVQV 98
            ++AR  + +
Sbjct: 92  DRIARESLSL 100

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6P6UBL08.9e-0428.97uncharacterized protein LOC113709083 OS=Coffea arabica OX=13443 GN=LOC113709083 ... [more]
A0A2N9I6098.9e-0431.71Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS47740 PE=4 SV=1[more]
A0A2N9EI798.9e-0432.98Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2337 PE=4 SV=1[more]
A0A2N9EM298.9e-0432.98Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS3764 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_017250619.11.5e-0536.54PREDICTED: uncharacterized protein LOC108221234 [Daucus carota subsp. sativus][more]
XP_042974784.12.8e-0434.04uncharacterized protein LOC122306423 [Carya illinoinensis][more]
XP_021722392.18.2e-0427.68uncharacterized protein LOC110689869 [Chenopodium quinoa][more]
XP_042962672.18.2e-0432.98uncharacterized protein LOC122296942 [Carya illinoinensis][more]
XP_042969199.18.2e-0434.62uncharacterized protein LOC122301911 [Carya illinoinensis][more]
Match NameE-valueIdentityDescription
AT5G42965.11.8e-0432.86Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 10..93
e-value: 5.2E-14
score: 52.1
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 1..96
score: 9.230108
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 10..98
e-value: 6.4E-13
score: 50.8
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 10..92
e-value: 4.14345E-14
score: 61.5612
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 11..96

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g006230.1Lcy10g006230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity